EP3205403A1 - Vorrichtung und verfahren zum dynamischen prüfen von blutinteraktionseigenschaften von flachmaterialien - Google Patents

Vorrichtung und verfahren zum dynamischen prüfen von blutinteraktionseigenschaften von flachmaterialien Download PDF

Info

Publication number
EP3205403A1
EP3205403A1 EP16154803.7A EP16154803A EP3205403A1 EP 3205403 A1 EP3205403 A1 EP 3205403A1 EP 16154803 A EP16154803 A EP 16154803A EP 3205403 A1 EP3205403 A1 EP 3205403A1
Authority
EP
European Patent Office
Prior art keywords
blood
sample
fluid channel
material sample
test chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16154803.7A
Other languages
English (en)
French (fr)
Inventor
Cecile Boudot
Markus Eblenkamp
Erich Wintermantel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technische Universitaet Muenchen
Original Assignee
Technische Universitaet Muenchen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technische Universitaet Muenchen filed Critical Technische Universitaet Muenchen
Priority to EP16154803.7A priority Critical patent/EP3205403A1/de
Publication of EP3205403A1 publication Critical patent/EP3205403A1/de
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/23—Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation

Definitions

  • the present invention is in the field of biomedical technology.
  • the present invention relates to the dynamic evaluation of the hemocompatibility of materials.
  • the conditions under which such blood interaction properties tests must be carried out are regulated by the DIN EN ISO 10993-4 norm, which recommends the use of dynamic evaluations, which reproduce better the real physiological flow conditions inside the body.
  • the norm does not describe a particular test bench with which the tests should be carried out.
  • Several kinds of testing systems are available. Most of the currently available systems for dynamic evaluation of hemocompatibility have serious limitations, especially regarding restricted ability to change the tested materials and poor access to the test surface of the same. Further problems include the unwanted influencing of the results of the experiment due to damage of blood cells during the test.
  • testing system overcoming the deficiencies of most current systems could furthermore pave the way to standardised testing, like for example for dynamic hemocompatibility testing in the DIN norm.
  • US 2011/0045993 discloses a microfluidic device for testing the interaction between a fluid sample and a test material comprising a sample inlet, a sample outlet and a flow cavity extending between the sample inlet and the sample outlet for flow of a fluid sample through the flow cavity.
  • the flow cavity comprises a test area to which at least one test material can be attached.
  • US 2014/0287451 describes a flow chamber having an inlet port, an exit port and a flow channel extending between the inlet port and the exit port.
  • the fluid channel is configured to hold a sample so that a fluid flowing from the inlet port to the exit port through the flow chamber contacts a side of the sample.
  • the test chamber further has an optically transparent viewing window adjacent to the flow channel so that the sample can be observed in real-time while it is exposed to the fluid.
  • the problem underlying the invention is to provide means and a method for dynamically testing blood interaction properties of a sample material with reduced damage of blood cells during the test and the possibility to easily change the sample material. This problem is solved by an apparatus according to claim 1 and a method according to claim 10.
  • the apparatus of the invention comprises at least one test chamber for receiving a blood sample.
  • blood sample is understood to have a broad meaning and covers any liquid substance comprising at least parts of human or animal blood.
  • the test chamber comprises at least two reservoirs, a fluid channel extending between the at least two reservoirs and a receptacle for receiving the material sample.
  • the receptacle might for example be any geometric configuration allowing for the arrangement of the material sample in the test chamber, like but not restricted to, an indent or a depression.
  • the receptacle is arranged with respect to the fluid channel such that when the blood sample is received in the test chamber and the material sample is received in the receptacle, the blood sample flowing in the fluid channel may flow in contact with the material sample.
  • test chamber has been chosen to indicate that the reservoirs and the fluid channel form a hollow structure for accommodating the blood sample, but shall not imply any further structural limitations. In particular, it does not imply that the reservoirs and the fluid channel are necessarily rigidly connected. Instead, the reservoirs and the fluid channel could for example be connected by flexible tubes or the like.
  • the apparatus further comprises a driving arrangement configured for providing a reciprocating movement of at least a part of the at least one test chamber such as to alternatingly raise one of the reservoirs with respect to the other, thereby causing the blood sample in the fluid channel to flow back and forth from one reservoir to the other.
  • the driving arrangement can be any type of mechanical, magnetic, electric or electronic system or any combination thereof suitable for providing the reciprocating movement of the test chambers or parts thereof.
  • the reciprocating movement of the test chamber provided by the driving arrangement alternatingly raises one of the reservoirs with respect to the other such that gravity causes the blood sample in the fluid channel to flow in contact with the material sample alternatingly from the momentarily higher reservoir to the momentarily lower reservoir.
  • the blood sample interacts dynamically with the material sample, thereby simulating the physiological conditions inside true blood vessels.
  • Both reservoirs typically have the same form and volume.
  • the reservoirs might have a cylindrical form, although other geometries are possible.
  • the reservoirs may be connected to the fluid channel through at least one opening.
  • the reservoirs may be open at their upper end so as to allow the fluid to flow in the fluid channel freely under the influence of gravity without any restriction due to a pressure gradient in the test chamber.
  • the amount of the blood sample in the test chamber is chosen such that the volume of the blood sample is bigger than the added volume of the fluid channel and of one of the reservoirs and smaller than the added volume of the fluid channel and of both reservoirs.
  • the fluid channel is completely filled with the blood sample, while the excess volume of the blood sample not fitting in the fluid channel partially fills the reservoirs.
  • both reservoirs have the same volume and one of the reservoirs is raised with respect to the other, a larger amount of the blood sample accumulates in the reservoir momentarily having the lower position.
  • both reservoirs are arranged equally high, both reservoirs are partially filled with the same amount of the blood sample.
  • the volume of the reservoirs is preferably big enough so as to guarantee that no blood sample flows out of the test chamber during the operation of the apparatus. Other arrangements regarding the volume of the blood sample, the fluid channel and the reservoirs are possible, so as to enable different flowing configurations.
  • the driving arrangement comprises a rocking platform or a rocker that provides a reciprocating tilting movement of the at least one test chamber.
  • the at least one test chamber is then placed or arranged on the platform of the rocking platform.
  • the adjustment of the frequency and maximum angle of the reciprocating tilting movement of the test chamber driven by the rocking platform allows for an easy and direct control of the parameters determining the flow conditions of the blood sample in the test chamber, such as the flow rate or the wall shear rate.
  • the reciprocating tilting movement of the test chamber driven by the driving arrangement is typically a tilting of the test chamber with respect to a horizontal rest position, wherein the tilting movement takes place in a vertical plane.
  • This vertical plane preferably contains the direction corresponding to the longitudinal direction of the test chamber.
  • the longitudinal direction of the test chamber and the longitudinal direction of the fluid channel coincide, such that the instant axis of rotation of the tilting movement of the test chamber is perpendicular to the longitudinal direction of the fluid channel.
  • a fluid flow inside the fluid channel only occurs along the longitudinal direction of the fluid channel.
  • other configurations might be used.
  • the at least one test chamber comprises a first part and a second part, which are releasably secured to one another, for example by means of screws or other means for pressing the parts together.
  • means may be provided for fixing the at least one test chamber to the driving arrangement.
  • the receptacle is configured for receiving a planar material sample.
  • the planar geometry of the receptacle allows for an easy operation of the apparatus when it comes to placing, exchanging or removing the material sample and reduces the time needed for preparation or manipulation of the apparatus. It further provides easy and direct access to the test surface of the material sample, both before and after it has interacted with the blood sample in the apparatus and hence facilitates the investigation of said test surface.
  • test surface refers to the surface of the material sample facing the inside of the fluid channel. This enables for example the detailed characterization of the chemical composition, the structure, the coating or the roughness of the test surface of the material sample before its contact with the blood sample. It further allows investigation of the blood components adhered to the test surface after the material sample and the blood sample have interacted in the apparatus.
  • the planar material sample to be received in the receptacle has the form of a stripe.
  • a stripe is understood as having a generally rectangular shape with one of its dimensions significantly longer than the other one, for example at least three times longer. This allows for the use of planar material samples with standardized geometry, dimensions and structure, thereby offering a better control over the universe of possible test objects for analysis and comparison.
  • such a stripe-like shape contributes to the reduction of the influence of the apparatus upon the results of testing by allowing for a very reproducible washing procedure of the material samples.
  • Washing steps are commonly employed in the course of testing and in previously known testing systems they are typically performed by pipetting a wash solution on the sample, which may lead to uncontrolled detachment of adhered cells from the surface of the material sample due to strong shear stress and hence to uncontrolled changes in the cell concentrations on the surface of the material sample.
  • the at least one test chamber comprises a top confining part and a bottom confining part, wherein the top confining part and the bottom confining part can be tightly and releasably secured to one another and the receptacle is arranged within the bottom confining part, such that when the material sample is received in the receptacle and the top confining part and the bottom confining part are tightly secured to one another, a cavity between the top confining part and the material sample is formed that acts as the fluid channel.
  • the top confining part of the test chamber is preferably made of silicone rubber, although other material compositions are possible.
  • the bottom confining part may be formed by the second part of the at least one test chamber.
  • the blood sample when the blood sample is introduced into the test chamber and made to flow in the fluid channel, the blood sample flows between top confining part of the test chamber and the material sample.
  • This configuration reduces the contact of the blood sample with surfaces not to be tested, which for example suppresses unwanted blood activation and uncontrolled interactions.
  • This preferred embodiment further allows for configurations in which, provided a suitable amount of the blood sample is filled into the test chamber, the material sample that constitutes the bottom part of the fluid channel is entirely covered with the blood sample and hence is not in contact with air during the blood interaction properties test, thereby eliminating possible uncontrolled effects that could be caused by the interaction with air.
  • the apparatus further comprises a transition region between the fluid channel and each of the reservoirs, said transition region comprising rounded edges to prevent or at least reduce the formation of eddies in the blood sample flow in said transition region.
  • the prevention or reduction of the formation of eddies in the blood sample contributes to avoid unwanted blood activation like clotting, a better control of the testing conditions and the flow parameters and allows for testing the material sample under conditions that better reproduce physiological flow conditions.
  • the driving arrangement is adapted for simultaneously providing the reciprocating tilting movement of at least two, preferably of at least four and most preferably of at least six test chambers. This makes it possible to test several material samples simultaneously thereby reducing the time required for testing different material samples.
  • the driving arrangement is a rocking platform
  • at least some of the test chambers might be stapled on top of each other on the platform of the rocking platform and optionally secured thereto.
  • the fluid channel has a volume between 2.5 cm 3 and 20 cm 3 , preferably between 3.0 cm 3 and 12 cm 3 , and most preferably between 4.5 cm 3 and 8 cm 3 .
  • the fluid channel has a rectangular cross-section. This geometry of the fluid channel makes it possible to approximately resemble physiological flow conditions inside blood vessels while preserving the ability to easily change the material sample and the direct access to the receptacle, the material sample and the test surface of the latter.
  • the cross-section of the fluid channel has a width to height ratio between 1 and 40, preferably between 10 and 30 and most preferably between 15 and 25.
  • the fluid channel has a cross-section between 10 mm 2 and 30 mm 2 . This has the advantage of maximising the contact surface of the material sample with respect to a given cross-section of the fluid flow.
  • a further aspect of the invention relates to a method for evaluating blood interaction properties of a material sample comprising the steps of:
  • the step of providing a material sample comprises preheating the material sample to a temperature between 35°C and 42°C, preferably between 36°C and 38°C, so as to reproduce physiological conditions.
  • the method may further comprise, prior to step b), a step for preparing the blood sample.
  • a step for preparing the blood sample This may for example comprise mixing blood with an anticoagulant or preparing a blood sample with a predefined cell concentration so as to minimise the donor-dependent factor influencing the result of the evaluation.
  • This temperature is preferably maintained during the blood interaction properties evaluation procedure.
  • the method may further comprise, prior to step b), a step of measuring of properties of the blood sample before interaction with the material sample in the apparatus.
  • this may comprise measuring a cell concentration of the blood sample.
  • Step c) of operating the driving arrangement may comprise configuring it to provide the reciprocating movement of the at least one test chamber according to predefined parameters, like for example reciprocating frequency, maximum height of the reservoirs or operation time. Said parameters might be constant or variable in time.
  • the reciprocating frequency refers to the rate at which each of the reservoirs alternatingly occupies the highest attainable position with respect to a horizontal rest position and may be measured in revolutions per minute (rpm) according to the number of times one of the reservoirs successively occupies the highest position in a period of time of one minute.
  • the maximum height refers to the maximum height with respect to a horizontal rest position.
  • the parameters determining the flow conditions of the blood sample in the fluid channel might be the reciprocating frequency and the maximum tilt angle.
  • the maximum tilt angle refers to the maximum tilt angle of the at least one test chamber with respect to the horizontal rest position, that is the angle between the longitudinal axis of the test chamber and the horizontal rest position when one of the reservoirs has achieved the highest attainable position.
  • step c) of operating the driving arrangement may comprise configuring said driving arrangement to provide the reciprocating tilting movement of the at least one test chamber with a reciprocating frequency between 2 and 50 rpm and a maximum tilt angle between 1 and 20 degrees or a maximum height of the reservoirs that corresponds to such maximum tilt angle.
  • step c) of operating the driving arrangement may comprise configuring said driving arrangement to operate for a period of time between 1 and 240 minutes, preferably between 60 and 90 minutes. This has been experimentally determined to be long enough for the interaction of the blood sample with the material sample to have measureable consequences and hence to ensure good simulation of the in-vivo application of the material sample.
  • step d) of performing an analysis of one or both of the material sample and the blood sample after the material sample and the blood sample have interacted in the apparatus may comprise any kind of analysis aiming at characterizing the blood interaction properties of the tested material.
  • said step may comprise one or more of
  • FIG. 1 shows a schematic representation of an apparatus 10 for testing blood interaction properties of a material sample according to a preferred embodiment of the invention.
  • the apparatus 10 comprises a test chamber 20 for receiving a blood sample and a driving arrangement 30.
  • the test chamber 20 comprises two reservoirs 21, a fluid channel 22 extending between the two reservoirs 21, and a receptacle 23 (see Fig. 2 and 3 ) for receiving a material sample, not shown in Fig. 1 .
  • the receptacle 23 is arranged with respect to the fluid channel 22 in such a way that when the blood sample is received in the test chamber 20 and the receptacle 23 receives the material sample, the blood sample flowing in the fluid channel 22 may flow in contact with the material sample.
  • the driving arrangement 30 is configured for providing a reciprocating movement of the test chamber 20 such as to alternatingly raise one of the reservoirs 21 with respect to the other, so that gravity causes the blood sample in the fluid channel 22 to flow in contact with the material sample in the receptacle 23 alternatingly from the momentarily higher reservoir to the momentarily lower reservoir.
  • the driving arrangement 30 can generally be any type of mechanical, magnetic, electric or electronic system or any combination thereof suitable for providing the reciprocating movement of the test chamber 20.
  • the driving arrangement 30 is symbolically represented by a triangle, which stands for any suitable arrangement, which in this particular case, comprises a platform 31 on which the test chamber 20 is placed and provides a reciprocating tilting movement of the test chamber 20.
  • the driving arrangement 30 might be a rocking platform or rocker comprising a platform on which several test chambers can be placed for being simultaneously driven.
  • Both reservoirs 21 have the same cylindrical shape and identical volume.
  • the reservoirs 21 are connected to the fluid channel 22 through at least one opening 210.
  • the transition region between the fluid channel 22 and each of the reservoirs 21 around the opening 210 comprises rounded edges, not shown in Fig. 1 (see Fig. 4 ), to prevent or at least to reduce the formation of eddies in a blood sample flowing in said transition region.
  • the reservoirs 21 have a second opening 212 to establish atmospheric pressure in the reservoirs 21, thereby allowing the fluid in the fluid channel 22 to flow freely under the influence of gravity.
  • the amount of the blood sample in the test chamber 20 is chosen such that the volume of the blood sample is bigger than the added volume of the fluid channel 22 and of one of the reservoirs 21 and smaller than the added volume of the fluid channel 22 and of both reservoirs 21.
  • the fluid channel 22 is completely filled with the blood sample, while the excess volume of the blood sample not fitting in the fluid channel partially fills the reservoirs 21.
  • the tilt angle of the test chamber 20 is different from 0, like in the top and the bottom situation displayed in Fig. 1 , a bigger amount of the blood sample accumulates in the reservoir momentarily having the lower position.
  • the tilt angle of the test chamber 20 is zero, as is the case in the middle situation displayed in Fig.
  • the test chamber 20 momentarily is arranged horizontally and both reservoirs 21 are partially filled with the same amount of the blood sample.
  • the fluid channel 22 has a rectangular cross-section with a width to height ratio of 14 and a volume of 4,5 cm 3 .
  • FIG. 2 shows an exploded view of the components of the test chamber 20 of Fig. 1 .
  • the test chamber 20 comprises a first part 201, a top confining part 220 of the fluid channel 22 and a second part 202 confining the fluid channel 22.
  • the second part 202 comprises the receptacle 23 for receiving a material sample 40.
  • the top confining part 220 of the fluid channel 22 is arranged, such that when the first part 201 and the second part 202 are secured to one another by means of screws 203, the top confining part 220 of the fluid channel 22 is clamped into the receptacle 23.
  • the base of the receptacle 23 in the second part 202 then constitutes a bottom confining part of the fluid channel 22, and a rectangular cavity 221, not seen in the exploded view of Fig. 2 is formed between the top confining part 220 of the fluid channel 22 and the second part 202, which constitutes the fluid channel 22.
  • a fluid flowing in the rectangular cavity 221 flows in contact with said material sample 40.
  • the material sample 40 received in the receptacle 23 has the form of a stripe.
  • the material sample 40 can be tightly secured to the top confining part 220 of the fluid channel 22 by securing together the first part 201 and the second part 202 of the test chamber 20, within which the top confining part 220 of the fluid channel 22 is arranged. The material sample 40 then entirely covers the receptacle 23, so that it forms the bottom of the fluid channel 22.
  • the top confining part 220 of the fluid channel 22 is made of silicone rubber.
  • Figure 3 shows a cross-sectioned and a perspective view of the test chamber 20 of Fig. 1 and 2 .
  • the screws 203 are holding together the first part 201 and the second part 202 of the test chamber 20.
  • the top confining part 220 is arranged within the first part 201 and the second part 202.
  • the material sample 40 is received in the receptacle 23 arranged within the second part 202.
  • Fig. 3 depicts a rectangular cavity 221, which is formed between the top confining part 220 and the material sample 40 through which the blood sample may flow, and which constitutes the fluid channel 22.
  • FIG. 4 displays in detail the top confining part 220 of the test chamber 20 of Fig. 2 and 3 .
  • the top confining part 220 comprises the reservoirs 21 and an elongated recess 222 along the longitudinal direction of the top confining part 220 extending between the reservoirs 21.
  • the second part 202 acts as a bottom confining part and a rectangular cavity 221 is formed between the material sample 40 and the top confining part 220, which forms the fluid channel 22.
  • Each of the reservoirs 21 is connected to the recess 222 through at least one opening 210.
  • the transition region between the recess 222 and each of the reservoirs 21 around the opening 210 comprises rounded edges, which help preventing the formation of eddies in the blood sample when the rectangular cavity 221 acts as the fluid channel 22.
  • the driving arrangement 30 was a rocking platform (Duomax 1030, Heidolph).
  • the fluid channel 22 of the test chamber 20 had a rectangular cross-section and a top confining part 220 of silicone rubber.
  • the receptacle 23 of the apparatus had a rectangular stripe-shape and the reservoirs 21 had rounded edges at the passage from the fluid channel 22 to the reservoirs 21 avoiding the formation of eddies in the blood sample.
  • the longitudinal axis of the test chamber 20 was aligned with the rocking platform such that the tilting movement be in the direction of said longitudinal axis. Up to 8 test chambers 20 were simultaneously loaded onto the rocking platform.
  • Substitute liquids were used in the preliminary assessment of the flowing conditions so as not to waste real blood for this purpose.
  • a mixture of 35% glycerine (glycerol anhydrous, Applichem GmbH, Germany) and 65% saline solution was used to simulate whole blood, and distilled water was used to simulate platelet suspension.
  • These fluids were chosen because the viscosity and density are similar to those of the corresponding true biological fluids.
  • Their kinematic viscosity was measured with a capillary viscometer (Ubbelohde AVS 310, Schott-Gerate GmbH, Germany) and the density was measured with a density meter (DensityMeter DMA 4100M, Anton Paar GmbH, Austria).
  • the glycerine solution and the distilled water were respectively pipetted into a 2-mm-high and a 1-mm-high fluid channel. Two different kinds of fluid channel were used due to the different viscosities of whole blood and the platelet suspension.
  • the test chambers were placed on the rocking platform and this was configured to operate with a maximum tilt angle of 5°. By adding coloured fluid in one of the reservoirs, it was possible to visualise the velocity profile of the fluids for tilting frequencies varying between 2 and 50 rpm. The velocity of the fluid in the centre-line of the fluid channel ( v 0 ) was recorded over time.
  • K 2 is another correction factor that adapts the amplitude of the periodic flow depending on the reciprocating frequency and is a function of the Womersley number.
  • K 2 was calculated using the formula of Loudon and Tordesillas shown in The use of the dimensionless Womersley number to characterise the unsteady nature of internal flow. J. theor. Biol. 1998;191:63-78 . Assuming the ideal case of a Poiseuille flow between two plates, the constants K 1 and K 2 were taken to be 1. This assumption was found to be justified for tilting frequencies over 7.5 rpm.
  • the main flow velocity of the test fluids over a tilting period corresponded to a sinus curve and the maximum amplitude v max obtained during a tilt movement was found to depend on the reciprocating frequency.
  • the highest values of v max were 11.3 cm/s at 30 rpm for the glycerine solution in the 2-mm-high fluid channel and 6.3 cm/s at 10 rpm for distilled water in the 1-mm-high fluid channel.
  • the inertia of the fluid limited a higher flow velocity.
  • the measured density and viscosity of the test fluids were comparable to those of whole blood and platelet suspension so that the flow characteristics in the fluid channel should be approximately equivalent.
  • the maximum Reynolds number was 120, which guarantees laminar flow conditions for all configurations and tilting velocities.
  • the Wormersley number ⁇ was smaller than 1 for all configurations at 30 rpm, which guaranteed quasi-steady flow conditions for tilting frequencies up to 30 rpm.
  • the obtained wall shear rate ⁇ for whole blood and the platelet suspension depending on the reciprocating frequency is represented in figure 5 . In both cases the shear rate increased with the reciprocating frequency up to a maximum value of 339 s -1 for whole blood and 380 s -1 for the platelet suspension.
  • the flow conditions in the fluid channel corresponded to those characteristic of veins and arteries.
  • the resulting overlying supernatant and buffy coat concentrating most of the white blood cells and platelets were separated and underwent a second centrifugation at 2250 g for 9 minutes. Then the supernatant was removed and the resulting concentrated platelet suspension was adjusted to a concentration of 600 ⁇ 10 3 platelets/ ⁇ l (PLT/ ⁇ l) using phosphate buffered saline.
  • Planar material samples having the form of a stripe of low-density polyethylene (PE, type DOWLEXTM 2107GC, Dow Chemical Company, USA), polypropylene (PP, type PCGH10, Sabic, Saudi Arabia), polyoxymethylene (POM, type HOSTAFORM® MT24U01, Celanese Corporation, USA) and silicone (type SILPURAN® UR 9030/60, Wacker Chemie AG, Germany) were used.
  • the material samples of those four polymers were cleaned with 70%-2-propanol and vacuum dried for a minimum of 12 hours.
  • the surface of the material samples was characterised before conducting the hemocompatibility tests in order to compare the results with values from the literature.
  • the contact angle with water was measured to examine the wetting properties of the material samples with respect to aqueous liquids (Device OCA 20, DataPhysiy Instruments GmbH, Germany). For each material, ten measurements were conducted immediately after wetting with 2 ⁇ l drops. Topology and roughness of the surfaces of the material samples were assessed with a confocal microscope ( ⁇ Surf, NanoFocus Ag, Germany).
  • the material samples were introduced into the receptacle of the test chambers with 2-mm-high fluid channels, which were then preheated to 37°C.
  • the test chambers were filled with 8 ml of whole blood and then placed on the rocking platform for 60 minutes to operate according to the flow parameters indicated above at a constant temperature of 37°C. After that, the incubated blood samples were removed from the test chambers and the cell concentration was immediately measured. Three measurements were made for each material sample.
  • a reduction of the number of platelets in the blood after incubation is linked to thrombogenic events such as the adhesion of platelets on the surface of the material samples and the formation of platelet aggregates.
  • thrombogenic events such as the adhesion of platelets on the surface of the material samples and the formation of platelet aggregates.
  • each value was normalised with the mean value of the platelet count for all materials after testing.
  • test chambers having 1-mm-high fluid channels filled with 4.5 ml of platelet suspension.
  • blood platelets could adhere to the surface of the material samples.
  • the platelet suspensions were removed from the test chambers and 5 ml of phosphate buffered saline heated to 37°C was pipetted into one reservoir, made to flow through the fluid channel and removed from the other reservoir. This guaranteed that all samples underwent exactly the same wash procedure and only the adhered platelets remained on the surface of the material samples.
  • lysis buffer solution Sodium Citrate Tribasic Dihydrate 0.1 M, p-Nitrophenyl-Phosphat 5 mM, Triton X-100 1%, pH 5.4
  • the chemicals for the lysis buffer were all purchased from Sigma-Aldrich Co. LLC, USA.
  • the purpose of the lysis buffer is that of dissolving the adhered platelets and releasing the enzyme ACP (Acid Phosphatase) which reacts with p-Nitrophenyl-Phosphat resulting in a colour change.
  • the colour change is proportional to the number of thrombocytes dissolved in the lysis buffer and may be measured by means of UV spectroscopy so as to quantify the amount of platelets adhered to the surfaces of the material samples.
  • the reaction was stopped with 210 ⁇ l sodium hydroxide (NaOH 2 mol/L, Merck KGaA, Germany), and 170 ⁇ l of the solution was pipetted in a 96-wells microtiter plate.
  • the photometric absorbance of 170 ⁇ l of the solution was measured at 405 nm with an ELISA reader (Multiscan FC, Type 357, Thermo Fisher Scientific AG, USA).
  • ELISA reader Multiscan FC, Type 357, Thermo Fisher Scientific AG, USA.
  • a standard curve was applied which was generated using a dilution series of platelet suspensions.
  • test chambers were placed on a separate rocking platform configured to operate with a maximum tilt angle of 5° and a reciprocating frequency of 30 rpm.
  • One test chamber was left under static conditions. The temperature was kept at 37°C and after 30 minutes, 60 minutes and 90 minutes, blood was collected from one of the dynamically incubated chambers. Blood from the statically incubated chamber was collected after 90 minutes.
  • the amount of free haemoglobin in the obtained plasma of the blood samples was measured. For this purpose, the collected blood samples were centrifuged at 1000 g for 10 minutes. Plasma was drawn off and recentrifuged at 1000 g for another 10 minutes.
  • Hemoglobin has three absorbance peaks at 451 nm, 541 mm and 577 nm. Because of the small amounts of hemoglobin in plasma, the quantification method described by Harboe in "A method for determination of hemoglobin in plasma by near-ultraviolet spectrophotometry. Scandinav. J. Clin. & Lab. Investigation 1959;11:66-70 " based on the analysis of the highest peak at 450 nm was chosen.
  • the analysis of hematocrit measurements indicated a reduction of the number of platelets in the blood samples during incubation in the apparatus according to an embodiment of the invention. This can be due to platelet adhesion on the surface of the material sample and to activation or aggregation of platelets in the flowing blood samples.
  • Figure 6 shows the reduction in the number of platelets after contact with the four different kinds of material sample. The only significant difference in the platelet deprivation was found between PP and silicone, were silicone was the material with the least depraved platelets and PP the one which caused the highest platelet deprivation, 37% more platelet reduction than on silicone. The results showed significant differences for most of the test materials.
  • the amount of detected platelets per mm 2 surface area (PLT/ mm 2 ) of material sample varied between 17,000 PLT/ mm 2 for silicone and 4000 PLT/ mm 2 for POM.
  • PP and PE have intermediate amounts of adhered platelets on their surface with respectively 6000 PLT/ mm 2 and 12000 PLT/ mm 2 .
  • the most platelets were found on the silicone material sample and they exhibited an advanced stage of adhesion with spreading of pseudopodia and formation of platelet aggregates. No aggregates were found on the material samples of PE, PP and POM, and the cells were observed to be isolated and dispersed on the surface. More platelets were found on PE than on PP and in both cases they were strongly activated and spread numerous pseudopodia. A smaller amount of platelets was found on POM and they were less activated than on other materials.
  • Figure 8 shows the difference in the platelet adhesion when the same cell suspension is simultaneously incubated under static (0 rpm) or dynamic (15 rpm) flow conditions. Very few platelets were detected after incubation under static flow conditions and variations between the different test materials were minimal, from 1660 PLT/ mm 2 for silicone to 2020 PLT/ mm 2 for PP. However, after incubation the amount of adhered platelets increased greatly and pronounced differences between the different test materials could be measured, from 2800 PLT/ mm 2 for POM to 21,700 PLT/ mm 2 for silicone. This confirms that the detection of platelets was absolutely caused by the flow-induced adhesion of the platelets on the surface of the material samples and excludes the possibility that the detected platelets came from residual blood which may not have been removed by the washing procedure.
  • the levels of plasma hemoglobin detected after incubation of the blood samples in the test chambers and the corresponding index of hemolysis are shown in Fig. 9 .
  • the amount of free hemoglobin in plasma was 0.2 mg/dl and after 90 minutes static incubation in the apparatus, the amount of plasma hemoglobin was 0.6 mg/dl. The difference between the two values was presumably due to the blood damage by pipetting.
  • the amount of plasma hemoglobin for the dynamically incubated blood samples ranged between 0.5 and 1.5 mg/dl, which is still in the lower range of free hemoglobin in blood plasma for healthy subjects (0 mg/dl to 10 mg/dl).
  • Hemoglobin release in plasma was slightly elevated by high shear rates (340 s -1 at 30 rpm, arterial flow conditions) with respect to low shear rates (135 s -1 at 10 rpm, venous flow conditions). A time dependence of the hemoglobin release could also be observed and the maximum plasma hemoglobin (1.42 mg/dl) was reached after 90 minutes at 30 rpm.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP16154803.7A 2016-02-09 2016-02-09 Vorrichtung und verfahren zum dynamischen prüfen von blutinteraktionseigenschaften von flachmaterialien Withdrawn EP3205403A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP16154803.7A EP3205403A1 (de) 2016-02-09 2016-02-09 Vorrichtung und verfahren zum dynamischen prüfen von blutinteraktionseigenschaften von flachmaterialien

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP16154803.7A EP3205403A1 (de) 2016-02-09 2016-02-09 Vorrichtung und verfahren zum dynamischen prüfen von blutinteraktionseigenschaften von flachmaterialien

Publications (1)

Publication Number Publication Date
EP3205403A1 true EP3205403A1 (de) 2017-08-16

Family

ID=55527725

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16154803.7A Withdrawn EP3205403A1 (de) 2016-02-09 2016-02-09 Vorrichtung und verfahren zum dynamischen prüfen von blutinteraktionseigenschaften von flachmaterialien

Country Status (1)

Country Link
EP (1) EP3205403A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113049508A (zh) * 2021-03-11 2021-06-29 中国食品药品检定研究院 一种透析器中空纤维的溶血试验方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110045993A1 (en) 2008-02-05 2011-02-24 Nigel Kent Microfluidic device for assessing object/test material interactions
US20130004964A1 (en) * 2009-09-21 2013-01-03 Roche Diagnostics Operations,Inc. Method for carrying out reactions in an analytical device
US20140287451A1 (en) 2011-12-09 2014-09-25 University Of Florida Research Foundation, Incorporated Flow chambers, methods of using the flow chamber, and methods of making the flow chamber
JP2015203610A (ja) * 2014-04-14 2015-11-16 株式会社アペレ 血液凝固時間測定装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110045993A1 (en) 2008-02-05 2011-02-24 Nigel Kent Microfluidic device for assessing object/test material interactions
US20130004964A1 (en) * 2009-09-21 2013-01-03 Roche Diagnostics Operations,Inc. Method for carrying out reactions in an analytical device
US20140287451A1 (en) 2011-12-09 2014-09-25 University Of Florida Research Foundation, Incorporated Flow chambers, methods of using the flow chamber, and methods of making the flow chamber
JP2015203610A (ja) * 2014-04-14 2015-11-16 株式会社アペレ 血液凝固時間測定装置

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"A method for determination of hemoglobin in plasma by near-ultraviolet spectrophotometry", SCANDINAV. J CLIN. & LAB. INVESTIGATION, 1959, pages 66 - 70
"The use of the dimensionless Womersley number to characterise the unsteady nature of internal flow", J. THEOR. BIOL., vol. 191, 1998, pages 63 - 78
BRUSS: "Theoretical Microfluidics", 2009, OXFORD UNIVERSITY PRESS, pages: 37 - 70
M. SANAK ET AL: "Assessment of hemocompatibility of materials with arterial blood flow by platelet functional tests", BULLETIN OF THE POLISH ACADEMY OF SCIENCES. TECHNICAL SCIENCES, vol. 58, no. 2, 1 January 2010 (2010-01-01), PL, XP055297290, ISSN: 0239-7528, DOI: 10.2478/v10175-010-0029-z *
VAN OEVREN W ET AL.: "Comparison of Modified Chandler, Roller Pump and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogeneicity Testing of Different Materials for Vascular Applications", INTERNATIONAL JOURNAL OF BIOMATERIALS, 2012
WIM VAN OEVEREN ET AL: "Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications", INTERNATIONAL JOURNAL OF BIOMATERIALS, vol. 20, no. 12, 1 January 2012 (2012-01-01), pages II38 - 7, XP055297208, ISSN: 1687-8787, DOI: 10.1016/S0735-1097(98)00348-9 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113049508A (zh) * 2021-03-11 2021-06-29 中国食品药品检定研究院 一种透析器中空纤维的溶血试验方法
CN113049508B (zh) * 2021-03-11 2023-03-07 中国食品药品检定研究院 一种透析器中空纤维的溶血试验方法

Similar Documents

Publication Publication Date Title
Li et al. Blood coagulation screening using a paper-based microfluidic lateral flow device
US7262059B2 (en) Systems and methods for measuring fluid properties
CA2660381C (en) Systems and methods for measuring fluid properties
US8256308B2 (en) Method and device for providing blood constituents
JPH03505702A (ja) 多分析物試験ビヒクル
KR20110056497A (ko) 혈소판 검사 방법 및 혈소판 검사 장치
EP3094252B1 (de) Blutprobenverwaltung mit offenzelligem schaummaterial
Song et al. Migration distance-based platelet function analysis in a microfluidic system
US20220187292A1 (en) Method and device for measuring the fibrinogen concentration in blood samples
EP3205403A1 (de) Vorrichtung und verfahren zum dynamischen prüfen von blutinteraktionseigenschaften von flachmaterialien
JP2019506592A (ja) 流体収集装置及びそれに関する方法
KR101749796B1 (ko) 혈소판 검사용 칩
WO2013045695A2 (en) Method for delivering a sample of body fluid to an analysing system, a syringe designed for use therein and a kit comprising such a syringe
US8559007B2 (en) Systems for measuring properties of a physiological fluid suspension
CN112924431B (zh) 一种微流体芯片及血小板功能检测装置
Nezafati Analyzing biomaterial surfaces and blood-surface interactions
EP0111551B1 (de) Verfahren und vorrichtung zum direkten und sofortigen messen der blutviskosität
Sarıyer et al. Time-and distance-resolved robotic imaging of fluid flow in vertical microfluidic strips: a new technique for quantitative, multiparameter measurement of global haemostasis
CN218350105U (zh) 一种血小板功能检测装置
NL2009403C2 (en) Method for determination of blood group and system for the same.
Zeng et al. Study of whole blood viscosity using a microfluidic device
CN115902268A (zh) 用于血小板检测的微芯片及使用该微芯片的血小板检测装置
Zhu et al. Blood Cell Separation Issues in Miniature Blood Diagnostic Kits
Einav Human Erythrocyte Filterability at Low Driving Pressure
JPH08292190A (ja) 血液検査容器

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20180217