EP3200611A1 - Procédé de fabrication d'une biomasse granulée contenant une matière valorisable sensible à l'oxydation - Google Patents

Procédé de fabrication d'une biomasse granulée contenant une matière valorisable sensible à l'oxydation

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Publication number
EP3200611A1
EP3200611A1 EP15766527.4A EP15766527A EP3200611A1 EP 3200611 A1 EP3200611 A1 EP 3200611A1 EP 15766527 A EP15766527 A EP 15766527A EP 3200611 A1 EP3200611 A1 EP 3200611A1
Authority
EP
European Patent Office
Prior art keywords
biomass
particulate
weight
aurantiochytrium
agglomeration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP15766527.4A
Other languages
German (de)
English (en)
Inventor
Christian Rabe
Mathias DERNEDDE
Alexander SCHRICKEL
Horst Priefert
Stefan Eils
Michael Diehl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
Original Assignee
Evonik Degussa GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa GmbH filed Critical Evonik Degussa GmbH
Publication of EP3200611A1 publication Critical patent/EP3200611A1/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/20—Agglomerating; Granulating; Tabletting
    • A23P10/22—Agglomeration or granulation with pulverisation of solid particles, e.g. in a free-falling curtain
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/37—Esters of carboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Definitions

  • the present invention relates to a process for the production of a granulated biomass containing an oxidation-sensitive valuable substance, as well as the granulated biomass obtainable by this process.
  • microbial cells for the production of valuable substances.
  • An example of such recyclables are food components, especially lipids such as polyunsaturated fatty acids.
  • lipids such as polyunsaturated fatty acids.
  • other fungi and algae play a special role in the production of such valuable substances.
  • PUFAs polyunsaturated fatty acids
  • the source of PUFAs was first and foremost fish. Furthermore, it is known that certain microbes produce heterotrophic PUFAs in large quantities, whereby the fatty acid production can be favorably influenced by the choice of specific reaction parameters.
  • the PUFAs can then be obtained from the cells or the cells can be used in the form of biomass directly in feed or food.
  • Spray drying of the biomass often produces a dusty and also poorly flowable product.
  • the contained valuable material should remain largely intact.
  • the workup should in particular also ensure that the cell membranes of the cells contained in the biomass are largely retained intact in order to prevent the oxidative degradation of the contained valuable material.
  • it should preferably also be prevented as far as possible that possibly freed valuable material, for example by oxidative degradation, is damaged.
  • the object according to the invention can be achieved by using a particulate starting biomass using a
  • Granulation agent is granulated. This procedure leads to a clearly defined, very easy to handle, flowable, dust-free and non-hygroscopic product, which is very well suited for incorporation in feed or foodstuffs.
  • a first subject of the present invention is therefore a process for producing a particulate biomass containing an oxidation-sensitive valuable material, characterized in that a particulate starting biomass using a
  • Granulation or granulation is subjected to granulation.
  • granulation means the transfer of a finely divided particulate powder into a coarser particulate powder, the coarse-grained powder obtainable preferably having a particle size of 0.1 to 2.0 mm (d50) and preferably a good one Has flowability.
  • the present invention thus also relates to a particulate biomass obtainable by a process according to the invention.
  • Another object of the present invention is therefore also a particulate biomass containing an oxidation-sensitive valuable material, characterized in that the particulate biomass contains an agglomeration aid.
  • the agglomeration aid to be used according to the invention is preferably selected from optionally modified carbohydrates, proteins, further organic
  • the optionally modified carbohydrate is an optionally modified polysaccharide, wherein as agglomeration aids preferably guar gum, gum arabic, guar gum, locust bean gum, xanthan gum, agar, carrageenan, starch, in particular corn starch, tapioca starch or potato starch, cellulose or their Derivatives, hemicellulose or their derivatives, alginic acid or maltodextrin, and mixtures thereof.
  • agglomeration aids preferably guar gum, gum arabic, guar gum, locust bean gum, xanthan gum, agar, carrageenan, starch, in particular corn starch, tapioca starch or potato starch, cellulose or their Derivatives, hemicellulose or their derivatives, alginic acid or maltodextrin, and mixtures thereof.
  • alkyl especially Ci-6-alkyl, especially Ci-4-alkyl
  • carboxyalkyl especially carboxy-Ci-6-alkyl, especially carboxy-Ci-4-alkyl-
  • Hydroxyalkyl especially hydroxy-Ci-6-alkyl, especially hydroxy-Ci-4-alkyl groups modified carbohydrates to understand.
  • cellulose modified as an agglomeration aid in particular by carboxy-modified cellulose, in particular carboxymethyl cellulose and / or hydroxypropylmethyl cellulose, very particularly preferably
  • Carboxymethyl cellulose The preferably used sodium salt of carboxymethyl cellulose is obtainable, for example, by reaction of sodium chloroacetate with
  • Alkali cellulose and commercially available under the trade name Blanose (Ashland, USA).
  • Modified celluloses which can be used according to the invention, in particular carboxymethyl celluloses, preferably have an average molecular weight of 80,000 to 800,000 g / mol, in particular 90,000 to 700,000 g / mol, particularly preferably 100,000 to 600,000 g / mol, in particular 150,000 to 400,000 g / mol, especially 200,000 to 300,000 g / mol.
  • maltodextrins are water-soluble carbohydrate mixtures obtainable by hydrolysis of starch. They are a mixture of monomers, dimers, oligomers and polymers of glucose. Depending on the degree of hydrolysis, the percentage composition differs. The degree of hydrolysis is given in dextrose equivalents (DE). Starch has a DE value of 1, glucose has a DE value of 100.
  • DE dextrose equivalents
  • Maltodextrins have a DE value of 3 to 20. According to the invention, maltodextrins be used with any DE values, but preferably a maltodextrin is used with a DE value of 3 to 10.
  • proteins can be used as agglomeration aids, in particular casein, gelatin, collagen, wheat gluten and mixtures thereof.
  • organic polymers can be used as agglomeration aids, in particular lignosulfonates, polymethylolcarbamides, polyacrylic acids or
  • inorganic substances can be used as agglomeration aids, in particular silicates, preferably bentonites, hexametaphosphate and
  • the silicate is preferably a neutral silicate.
  • the granulation is carried out according to the invention preferably at a product temperature of 40-60 ° C.
  • the granulation can be carried out using commercial granulators, for example using fluidized bed granulators, in particular using spouted bed, Siebêt- or
  • Granulators which can be used according to the invention
  • Glatt sold by the company Glatt (Binzen, Germany).
  • the granulator ProCell-LabSystem (Glatt, Germany) may be mentioned.
  • the agglomeration of the spray-dried particles is preferably carried out in one
  • the fine, preferably spray-dried, powder is passed into the fluidized bed and there the agglomeration aid - preferably in dissolved form, for example as an aqueous solution - sprayed and thus finely distributed in the fluidized bed.
  • the solvent is preferably evaporated by heating the incoming air.
  • the supply air velocity is in this case preferably adjusted so that a turbulence of the agglomerated particles is possible. This is achieved by regulating the supply air volume. If the agglomerates reach the desired particle size, they are no longer fluidized due to their weight and can be taken out of the bottom of the fluidized bed or at the lateral outlet of the continuous fluidized bed.
  • Fluidized bed thus adjusts itself to the rate of particle growth, since the particles grow from the fine, spray-dried particle to the larger agglomerate need to leave the fluidized bed.
  • the conditions to be used correspond to the standard conditions of thermal granulation.
  • the agglomeration aid is preferably present in an amount of from 0.05 to 6% by weight, in particular from 0.1 to 5.0% by weight, preferably from 0.2 to 4.5% by weight, in particular from 0.3 to 4.0% by weight, particularly preferably from 0.4 to 3.5% by weight, in particular from 0.5 to 3.2% by weight, in particular from 0.8 to 3.0% by weight .-%, in particular 1, 0 to 2.5 wt .-%, contained.
  • a particularly preferred biomass granules according to the invention contains carboxymethyl cellulose in an amount of 0.05 to 6 wt .-%, in particular 0.1 to 5 wt .-%, preferably 0.2 to 4.5 wt .-%, in particular 0.3 to 4.0 wt .-%, particularly preferably 0.4 to 3.5 wt .-%, in particular 0.5 to 3.2 wt .-%, especially 0.8 to 3.0 wt. -%, In particular 1, 0 to 2.5 wt .-%.
  • the agglomeration aid is metered into the process according to the invention in each case in an amount such that the stated wt .-% - proportion of
  • Agglomeration aids in the granules formed adjusts.
  • the biomass granules obtained when carrying out the granulation is preferably characterized in that the mean particle diameter (d50) is from 150 to 1000 ⁇ m.
  • the biomass granules obtained when carrying out the granulation are furthermore preferably characterized in that at least 80% by weight, in particular at least 90% by weight, particularly preferably at least 95% by weight, especially at least 98% by weight, the particles have a particle diameter (particle size) of 100 to 2000 ⁇ , preferably 100 to 1500 ⁇ , particularly preferably 100 to 1000 ⁇ have.
  • the particulate biomass used in the granulation process according to the invention can be obtained, for example, starting from biomass-containing fermentation broth by freeze drying or drum drying. However, it has turned out to be particularly advantageous according to the invention if particulate biomass obtained by spray-drying is used in the granulation process according to the invention. In spray drying, this is preferably
  • the spray drying can be carried out in a manner known to those skilled in the art, for example using a spray tower.
  • a nozzle spray drying in particular using a single-component nozzle or two-component nozzle, or drying using a rotating disk can be carried out.
  • An example is the spray dryer Production Minor Spray Dryer (GEA Niro, Müllheim, Germany).
  • a hydrophilic or hydrophobic silicate is used in the spray drying of the biomass to obtain the particulate starting biomass.
  • the hydrophilic or hydrophobic silicate can also be mixed in after the spray drying or else after the agglomeration.
  • the silicate is preferably used in an amount such that in the final granulation product a concentration of 0.05 to 5 wt.%, In particular 0.1 to 4 wt.%, Preferably 0.15 to 3.5 wt .-%, in particular 0.2 to 3.0 wt .-%, particularly preferably 0.25 to 2.5 wt .-%, in particular 0.3 to 2.0 wt .-%, especially 0.35 to 1, 8 wt .-%, in particular 0.4 to 1, 5 wt .-% adjusts.
  • a biomass granules according to the invention is therefore distinguished, in another preferred embodiment according to the invention, by the fact that, besides the
  • Agglomeration aids further silicate, in particular hydrophobic and / or hydrophilic silicate, preferably in the amount indicated above, contains.
  • the preparation of the particulate starting biomass according to the invention is preferably carried out starting from a fermentation broth containing the biomass.
  • the silicate is preferably only during the drying step or in the course of Drying method mixed with the fermentation broth or the still moist biomass. In the latter case, the silicate is preferably metered into the drying zone using a nozzle, preferably a two-substance nozzle.
  • the drying zone is the zone below the injection nozzle, through which the fermentation broth is metered.
  • the silicate can in this case be metered in suspended form, but is preferably metered in dry, in particular pulverulent, form.
  • Hydrophilic silicas are under the CAS no. 1 12926-00-8 registered and commercially available, for example, under the trade name Sipernat® (Evonik Industries, Germany).
  • Hydrophilic silicas preferably used according to the invention have a specific surface area (ISO 9277) of from 130 to 600 m 2 / g, preferably from 160 to 550 m 2 / g, and preferably a dioctyl adipate absorption value of from 1.5 to 4.0 ml / g, preferably 2.0-3.2 ml / g. They also preferably have a tamped density (not sieved, based on ISO 787-1 1) of 80 to 300 g / l, preferably 100 to 270 g / l.
  • the particle size of the hydrophilic silica (d50, laser diffraction, based on ISO 13320-1) is preferably 10 to 150 ⁇ m, in particular 15 to 130 ⁇ m.
  • the drying loss of the hydrophilic silica (2 hours at 105 ° C., based on ISO 787-2) is preferably not more than 10%, particularly preferably not more than 7%.
  • the loss on ignition of the hydrophobic silica (2 hours at 1000 ° C., based on ISO 3262-1) is preferably at most 10%, particularly preferably at most 6%.
  • the silicon dioxide content of the hydrophilic silica is preferably at least 95% by weight, more preferably at least 97% by weight (based on ISO 3262-19).
  • the pH of the hydrophilic silica (5% in water, based on ISO 787-9) is preferably from 5.0 to 7.0, more preferably from 6.0 to 6.5.
  • the hydrophilic silica is a product having a specific surface area of from 160 to 220 m 2 / g, a dioctyl adipate absorption value of from 2.0 to 2.8 ml / g, a tapped density of from 200 to 300 g / l and a particle size of 100 to 150 ⁇ .
  • a product is commercially available under the name Sipernat® 22 S (Evonik Industries, Germany).
  • the hydrophilic silica is a product having a specific surface area of from 450 to 550 m 2 / g, a dioctyl adipate absorption value of from 2.5 to 3.5 ml / g, a tapped density from 80 to 130 g / l and a particle size of 10 to 40 ⁇ .
  • a product is commercially available under the name Sipernat® 50 S (Evonik Industries, Germany).
  • Hydrophobic silicas are under the CAS no. 6861 1 -44-9 registered and commercially, for example, also under the trade name Sipernat® (Evonik Industries,
  • Hydrophobic silicas preferably used according to the invention have a methanol wettability of at least 40%, preferably at least 45%, particularly preferably at least 50%, in particular 40 to 65%, especially 50 to 60%.
  • They also preferably have a tamped density (not sieved, based on ISO 787-1 1) of 100 to 200 g / l, preferably 125 to 175 g / l.
  • a tamped density (not sieved, based on ISO 787-1 1) of 100 to 200 g / l, preferably 125 to 175 g / l.
  • hydrophobic silica (d50; laser diffraction, based on ISO 13320-1) is preferably 5 to 15 ⁇ m, in particular 8 to 12 ⁇ m.
  • hydrophobic silica (2 hours at 105 ° C., based on ISO 787-2) is preferably not more than 10%, more preferably not more than 6%.
  • the silicon dioxide content of the hydrophobic silica is preferably at least 95% by weight, more preferably at least 97% by weight (based on ISO 3262-19).
  • the carbon content of the hydrophobic silica is preferably at most 3.5 wt .-%, in particular at most 2 wt .-% (based on ISO 3262-19).
  • the pH of the hydrophobic silica (5% in a 1: 1 mixture of water and methanol, based on ISO 787-9) is preferably from 7 to 10.5, more preferably from 7.5 to 9.
  • the methanol wettability is a measure of the hydrophobicity of the silica powder. To determine this value, a certain amount of silica powder is weighed into water. The silica powder remains on the surface. The amount of methanol necessary for wetting the powder is then determined. Under
  • Methanol wettability is understood to mean the methanol content of a methanol-water mixture in% by volume, in which 50% of the hydrophobic silica sediment.
  • Hydrophobic silicas which can be used according to the invention are obtainable, for example, under the trade names Sipernat® D 10, Sipernat® D 15 and Sipernat® D 17 (Evonik Industries, Germany).
  • the fermentation broth used in the spray drying has before the start of
  • Spray drying preferably has a solids content of 10 to 50 wt .-% and according to a water content of 50 to 90 wt .-%,. If necessary, the fermentation broth is adjusted to this water content before the actual drying. This can be done in particular by centrifugation, flotation, filtration, in particular ultrafiltration or microfiltration, decantation and / or solvent evaporation.
  • Solvent evaporation is preferably carried out using a
  • Rotary evaporator a thin film evaporator or a falling film evaporator in a one-stage or multi-stage process.
  • the reverse osmosis for the purpose of narrowing the fermentation broth into consideration.
  • the fermentation broth is preferably concentrated to a solids content of at least 10 or 15% by weight, preferably of at least 20 or 25% by weight, in particular 10 to 50 or 15 to 45% by weight. -%, particularly preferably 15 to 40 wt .-% or 20 to 40 wt .-%.
  • biomass to be dried to the particulate starting biomass is preferably present before the spray drying in the form of a suspension with the previously stated solids content, wherein the suspension is preferably fermentation broth or concentrated fermentation broth.
  • the drying of the biomass is now preferably carried out by spray drying, in particular by spray drying or spray drying using a rotating disk.
  • the biomass may also be subjected to the drying step immediately after harvesting without prior concentration, in particular if the obtained
  • Fermentation broth already has a high solids content, preferably as stated above.
  • the primary drying, preferably spray drying, of the biomass is preferably to a residual moisture content of at most 10 wt .-%, in particular 0 to 10 wt .-%, more preferably at most 8 wt .-%, in particular 0.5 to 8 wt. -%, especially at most 6 or 5 wt .-%, in particular 0.5 to 6 or 0.5 to 5 wt .-%, dried.
  • the fermentation broth introduced is atomized into a defined drop size and dried with the introduced drying air in cocurrent. Since the individual droplets are separated from each other in this process, there is good heat and mass transport and thus efficient drying.
  • the particle size of the dried final product can be set defined.
  • hydrophilic or hydrophobic silicate is used in the spray-drying, it is preferably atomized in the drying zone in order to avoid adhering the drying particles to each other.
  • the silicate thus acts as a so-called anti-caking agent, whereby the setting of a defined and controllable particle size is facilitated.
  • drying gas can be passed over the biomass during spray-drying if desired in a cycle gas mode.
  • "Circular gas operation” means that the gas used for drying is circulated over the biomass in this case preferably has a temperature above the
  • the gas used is preferably air, more preferably air having a reduced oxygen content.
  • the gas conducted in the cycle gas mode preferably has an oxygen content of less than 20% by weight, preferably less than 15% by weight, in particular from 5 to 13% by weight.
  • the gas is preferably generated by passing the air over a burner and heating it in this way. At the same time, this reduces the oxygen content of the air to less than 20% by weight, preferably less than 15% by weight, in particular from 5 to 13% by weight.
  • the gas is always readjusted in the same way to a constant
  • the drying temperature in the nozzle spray tower can be adjusted to 95 ° C. because of the short residence times.
  • Solids content is understood to mean the mass remaining after complete removal of the water , which only crystallize out or precipitate on drying, the solids content being so far complementary to the water or moisture content.
  • the biomass-containing composition used in the drying is preferably the product of a fermentative cultivation process and is
  • to be used fermentation broth comprises in addition to the biomass to be dried
  • ingredients may be, in particular, salts, antifoams and unreacted
  • the silica and optionally the aforementioned further constituents of the fermentation medium and optionally partially released from the cells components are contained.
  • the further constituents of the fermentation broth may be partially separated before the biomass is dried, for example by solid-liquid separation, so that a product is produced during the drying which contains these further components of the fermentation broth, in particular salts, preferably in a quantity of not more than 20 Wt .-%, in particular not more than 15, 10 or 5 wt .-%, contains.
  • the cells contained in the biomass are preferably cells which contain a valuable substance, preferably an oxidation-sensitive valuable substance.
  • a valuable substance preferably an oxidation-sensitive valuable substance.
  • These may in particular be cells which naturally produce valuable substances, preferably lipids, in particular PUFAs (polyunsaturated fatty acids), but they may also be cells which are produced by appropriate genetic engineering
  • the production can be autotrophic, mixotrophic or heterotrophic.
  • the biomass preferably comprises cells which produce lipids, in particular PUFAs, heterotrophically.
  • the cells are preferably algae, fungi, in particular yeasts, or protists. Particularly preferred are cells of microbial algae or fungi.
  • Yeasts of Yarrowia, Candida, Rhodotorula, Rhodosporidium, Cryptococcus, Trichosporon and Lipomyces are particularly suitable as cells of oil-producing yeasts.
  • the biomass preferably comprises cells of the taxon
  • Labyrinthulomycetes (labyrinthulea, slime fungus, mucus), in particular those the family of Thraustochytriaceae.
  • the family of Thraustochytriaceae includes the genera Althomia, Aplanochytrium, Elnia, Japonochytrium, Schizochytrium,
  • the biomass particularly preferably comprises cells of the genera Thraustochytrium, Schizochytrium,
  • Aurantiochytrium or oblongichytrium especially those of the genus Aurantiochytrium.
  • the species Aurantiochytrium limacinum (formerly also called Schizochytrium limacinum) is preferred according to the invention.
  • Very particular preference is given to using the strain Aurantiochytrium limacinum SR21 ((IFO 32693) according to the invention.
  • the oxidation-sensitive valuable substance is preferably a
  • oxidation-sensitive lipid in particular an unsaturated fatty acid, more preferably a polyunsaturated fatty acid (PUFA) or highly unsaturated fatty acid (HUFA).
  • unsaturated fatty acid more preferably a polyunsaturated fatty acid (PUFA) or highly unsaturated fatty acid (HUFA).
  • PUFA polyunsaturated fatty acid
  • HUFA highly unsaturated fatty acid
  • the cells contained in the biomass are preferably characterized by having a valuable substance content, preferably lipid content, particularly preferably PUFA content, of at least 20% by weight, preferably at least 30% by weight, in particular at least 40% by weight .-%, in each case based on the cell dry matter, have.
  • a valuable substance content preferably lipid content, particularly preferably PUFA content
  • lipids are present in the form of triglycerides, preferably at least 50% by weight, in particular at least 75% by weight and in a particularly preferred embodiment at least 90% by weight of the lipids contained in the cell in the form of triglycerides.
  • the lipids contained in the cell preferably comprise polyunsaturated fatty acids (PUFAs), with preferably at least 10% by weight, in particular at least 20% by weight, particularly preferably from 20 to 60% by weight, in particular from 20 to 40% by weight.
  • PUFAs polyunsaturated fatty acids
  • polyunsaturated fatty acids are fatty acids which have at least two, in particular at least three, CC double bonds.
  • PUFAs polyunsaturated fatty acids
  • highly unsaturated fatty acids are preferred.
  • HUFAs are fatty acids which have at least four CC double bonds.
  • the PUFAs can be present in the cell in free form or in bound form. Examples of the presence in bound form are phospholipids and esters of PUFAs,
  • a majority of PUFAs is in the form of triglycerides, preferably at least 50 wt .-%, in particular at least 75 wt .-% and in a particularly preferred embodiment, at least 90 wt .-% of the PUFAs contained in the cell in the form of triglycerides available.
  • Preferred PUFAs are omega-3 fatty acids and omega-6 fatty acids, omega-3 fatty acids being particularly preferred.
  • Preferred omega-3 fatty acids are eicosapentaenoic acid (EPA, 20: 5 ⁇ -3), in particular the (5Z, 8Z, 1 1 Z, 14Z, 17Z) eicosaccharide.
  • Docosahexaenoic acid is particularly preferred.
  • a method for producing the biomass, in particular such biomass, which comprises lipids, in particular PUFAs, containing cells, in particular the order Thraustochytriales, are described in detail in the prior art (see, for example, WO91 / 07498, WO94 / 08467, WO97 / 37032, W097 / 36996, WO01 / 54510).
  • the preparation is usually carried out by culturing cells in the presence of a carbon source and a nitrogen source in a fermenter. It can biomass densities of more than 100 grams per liter and production rates of more than 0.5 grams of lipid per liter per hour can be achieved.
  • the process is preferably carried out as a so-called fed-batch process, i. that the carbon and nitrogen sources are supplied incrementally during the fermentation.
  • the lipid production can be induced after reaching the desired biomass by different means, for example by limiting the nitrogen source, the carbon source or the oxygen content or combinations thereof.
  • the fermentation of the cells is preferably carried out in a medium with low salinity, in particular to prevent corrosion.
  • This can be achieved in that, instead of sodium chloride, chlorine-free sodium salts, such as, for example, sodium sulfate,
  • Sodium carbonate, sodium bicarbonate or soda ash can be used as sodium source.
  • Chloride is preferably used in amounts of less than 3 g / l, in particular less than 500 mg / l, particularly preferably less than 100 mg / l, in the fermentation.
  • Carbon sources are both alcoholic and non-alcoholic carbon sources. Examples of alcoholic carbon sources are methanol, ethanol and isopropanol. Examples of non-alcoholic carbon sources are fructose, glucose, sucrose, molasses, starch and corn syrup.
  • Suitable sources of nitrogen include both inorganic and organic nitrogen sources. Examples of inorganic nitrogen sources are nitrates and ammonium salts, especially ammonium sulfate and ammonium hydroxide. Examples of organic
  • Nitrogen sources are amino acids, especially glutamate, and urea.
  • inorganic or organic phosphorus compounds and / or known growth stimulants such as yeast extract or corn steep liquor, may also be added to positively affect the fermentation.
  • the fermentation of the cells is preferably carried out at a pH of 3 to 1 1, in particular 4 to 10, and preferably at a temperature of at least 20 ° C, in particular 20 to 40 ° C, particularly preferably at least 30 ° C.
  • a pH of 3 to 1 1, in particular 4 to 10 and preferably at a temperature of at least 20 ° C, in particular 20 to 40 ° C, particularly preferably at least 30 ° C.
  • the biomass After completion of the fermentation, the biomass is harvested. Preferably, after harvesting the biomass, or possibly even shortly before harvesting the biomass, pasteurization of the cells occurs to kill the cells and to inactivate enzymes that might promote the breakdown of the lipids. The pasteurization takes place
  • the biomass preferably by heating the biomass to a temperature of 50 to 121 ° C for a period of 5 to 60 minutes.
  • antioxidants are BHT, BHA, TBHA, ethoxyquin, beta-carotene, vitamin E and vitamin C.
  • the antioxidant if used, is preferably added in an amount of from 0.01 to 2% by weight.
  • Particularly preferred according to the invention is a process for producing a particulate biomass, characterized in that a particulate starting biomass in
  • Labyrinthulomycetes in particular those of Family of Thraustochytriaceae, especially those of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium
  • Granulation agent is granulated so that the optionally modified
  • Polysaccharide in the final product in a concentration of 0.1 to 6 wt .-%, preferably 0.2 to 5 wt .-%, particularly preferably 0.5 to 4 wt .-%, especially 0.8 to 3 wt. -%, and wherein the biomass cells of the taxon Labyrinthulomycetes,
  • those of the family of Thraustochytriaceae especially those of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium contains.
  • a process for producing a particulate biomass characterized in that a particulate starting biomass is granulated in the presence of carboxymethyl cellulose as an agglomeration aid, so that the carboxymethyl cellulose in the final product in a concentration of 0, 1 to 6 wt .-%, preferably 0.2 to 5 wt .-%, particularly preferably 0.5 to 4 wt .-%, especially 0.8 to 3 wt .-%, is contained, and wherein the biomass Cells of the taxon
  • Labyrinthulomycetes especially those of the family Thraustochytriaceae, especially those of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or
  • Oblongichytrium in particular of the species Aurantiochytrium limacinum, especially of the strain Aurantiochytrium limacinum SR21.
  • a method for producing a particulate biomass characterized in that a particulate starting biomass is granulated in the presence of a maltodextrin, in particular a maltodextrin having a DE value of 3 to 10, as an agglomeration aid, so that the Maltodextrin in the final product in a concentration of 0.1 to 6 wt .-%, preferably 0.2 to 5
  • Wt .-% particularly preferably 0.5 to 4 wt .-%, especially 0.8 to 3 wt .-%, is contained, and wherein the biomass cells of the taxon Labyrinthulomycetes, especially those of the family of Thraustochytriaceae, especially those of genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium, in particular arose Aurantiochytrium limacinum, especially of the strain Aurantiochytrium limacinum SR21.
  • Labyrinthulomycetes especially those of the family of Thraustochytriaceae, especially those of genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium, in particular arose Aurantiochytrium limacinum, especially of the strain Aurantiochytrium limacinum SR21.
  • the particles produced by granulation according to the invention have excellent strength and have due to their low tendency to caking very good bulk and flow properties.
  • the particles also have a low
  • Granulation thus results in a free-flowing particulate product.
  • a product having the desired grain size can be obtained from the granules obtained by sieving or dust separation.
  • Free-flowing according to the invention is to be understood as meaning a powder which can flow out of a series of glass outlet vessels with differently sized outlet openings at least from the vessel with the opening 5 mm (Klein: Soaps, Oils, Fats, Wachse 94, 12 (1968)) ,
  • finely divided is meant according to the invention a powder with a predominant proportion (> 50%) of a particle size of 20 to 100 micrometers in diameter.
  • Dust-free according to the invention is to be understood as meaning a powder which only has small proportions ( ⁇ 10% by weight, preferably ⁇ 5% by weight, in particular ⁇ 3% by weight, especially ⁇ 1% by weight) of particle sizes below 100 microns.
  • the determination of the grain or particle sizes is carried out according to the invention preferably by methods of laser diffraction spectrometry.
  • the methods to be used are described in the textbook “Particle Size Measurement in Laboratory Practice” by R. H. Müller and R. Schuhmann, Academicliche Verlagsgesellschaft Stuttgart (1996) and in the textbook “Introduction to Particle Technology” by M. Rhodes, Verlag Wiley & Sons (1998). If different methods are applicable, preference is given to the first applicable methodology from the textbook of R.H. Müller and R. Schuhmann zur
  • the products obtained by granulation processes according to the invention preferably have a proportion of at least 80 wt .-%, in particular at least 90 Wt .-%, particularly preferably at least 95 wt .-%, especially at least 98 wt .-%, of particles having a particle size of 100 to 2000 micrometers, preferably 100 to 1500 micrometers, in particular 100 to 1000 micrometers.
  • the particulate starting biomass which is preferably obtainable by spray-drying processes preferably has a fraction of at least 80% by weight, in particular at least 90% by weight, particularly preferably at least 95% by weight, of particles having a particle size of from 100 to 500 micrometers. preferably 100 to 400 micrometers, especially 100 to 300 micrometers.
  • a structure is formed, which is recognizable as an agglomerate of smaller particles.
  • the particles according to the invention have a very low tendency to cake. This is presumably due to the agglomeration-related reduction in the surface-to-volume ratio of the particles.
  • the explicit addition of anti-caking agents - such as the aforementioned silicate - for the purpose of obtaining a free-flowing product can be completely dispensed with.
  • the proportion of dust i. Particles having a particle size of less than 100 micrometers, is preferably at most 10 wt .-%, in particular at most 8 or 6 wt .-% particularly preferably at most 4 wt .-%, in particular at most 2 wt .-%.
  • the bulk density of the products according to the invention is preferably from 350 to 550 kg / m 3 , particularly preferably from 350 to 500 kg / m 3 , in particular from 350 to 450, kg / m 3 .
  • the agglomerate-structured particles preferably have a nonspherical geometry.
  • at least 50 or 60% by weight, in particular at least 70 or 80% by weight, particularly preferably at least 90 or 95% by weight, in particular essentially all particles, are in non-spherical form.
  • non-spherical is to be understood as meaning that the diameter of a particle, starting from the center of mass of the particle, is not the same in all spatial directions, the deviation of the diameter of a particle starting from the center of mass of the particle being at least in relation to two spatial directions at least 20%, preferably at least 25%, more preferably at least 30%.
  • a particularly preferred object of the present invention is a particulate
  • Microns especially 100 to 1000 microns have.
  • a particulate biomass which contains an optionally modified polysaccharide in an amount of 0.05 to 6% by weight, in particular 0.1 to 5% by weight, preferably 0.2 to 4.5% by weight.
  • 0.4 to 4.0 wt .-% particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt .-%, especially 1, 0 to 3.0 Wt .-%, in particular 1, 5 to 2.5 wt .-% and cells of the taxon
  • Labynnthulomycetes especially those of the family Thraustochytnaceae, especially those of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or
  • Oblongichytrium in particular of the species Aurantiochytrium limacinum, especially of the strain Aurantiochytrium limacinum SR21, contains, wherein at least 80 wt .-%, in particular at least 90 wt .-%, preferably at least 95 wt .-%, of the particles contained a particle size of 100 to 2000 microns, preferably 100 to 1500
  • Microns especially 100 to 1000 microns have.
  • a particulate biomass which comprises a modified cellulose, in particular carboxymethyl cellulose, in an amount of from 0.05 to 6% by weight, in particular from 0.1 to 5% by weight, preferably from 0.2 to 4 , 5 wt .-%, in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt .-%, especially 1 , 0 to 3.0 wt .-%, in particular 1, 5 to 2.5 wt .-% and cells of the taxon Labynnthulomycetes, especially those of the family of Thraustochytnaceae, especially those of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium, in particular of the species Aurantiochytrium limacinum, especially of the strain Aurantiochytrium limacinum SR21,
  • Microns especially 100 to 1000 microns have.
  • a particulate biomass comprising a maltodextrin, in particular a maltodextrin having a DE value of from 3 to 10, in an amount of from 0.05 to 6% by weight, in particular from 0.1 to 5% by weight, preferably 0.2 to 4.5 wt .-%, in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt. %, especially 1, 0 to 3.0 wt .-%, in particular 1, 5 to 2.5 wt .-% and cells of the taxon Labyrinthulomycetes, in particular those of the family of
  • Thraustochytriaceae especially those of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium, in particular of the species Aurantiochytrium limacinum, in particular the strain Aurantiochytrium limacinum SR21, containing at least 80 wt .-%, in particular at least 90 wt .-%, preferably at least 95 Wt .-%, of the particles contained a particle size of 100 to 2000 micrometers, preferably 100 to 1500 micrometers, especially 100 to 1000 micrometers.
  • a particular subject of the present invention is a particulate biomass containing an optionally modified polysaccharide in an amount of 0.05 to 6 wt .-%, in particular 0.1 to 5 wt .-%, preferably 0.2 to 4.5 wt .-%, in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt .-%, especially 1, 0 bis 3.0 wt .-%, in particular 1, 5 to 2.5 wt .-% and cells of the taxon Labyrinthulomycetes, especially those of the family of Thraustochytriaceae, especially those of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium, especially the species Aurantiochytrium limacinum, especially of the strain Aurantiochytrium limacinum SR21, wherein at least 80 wt
  • Microns especially 100 to 1000 microns, wherein the biomass further comprises a hydrophilic or hydrophobic silicate in an amount of 0.05 to 6 wt .-%,
  • Cellulose in particular carboxymethyl cellulose, in an amount of 0.05 to 6 wt .-%, in particular 0.1 to 5 wt .-%, preferably 0.2 to 4.5 wt .-%, in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt .-%, especially 1, 0 to 3.0 wt .-%, in particular 1, 5 to 2.5 wt .-%, and includes cells of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or Oblongichytrium, in particular the species Aurantiochytrium limacinum, especially the strain
  • Aurantiochytrium limacinum SR21 wherein at least 80 wt .-%, in particular at least 90 wt .-%, preferably at least 95 wt .-%, of the particles contained a particle size of 100 to 2000 microns, preferably 100 to 1500 microns, especially 100 to 1000 micrometers, and further comprising a hydrophilic or hydrophobic silicate in an amount of 0.05 to 6 wt .-%, in particular 0.1 to 5 wt .-%, preferably 0.2 to 4.5 wt.
  • % in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt .-%, especially 1, 0 to 3, 0 wt .-%, in particular 1, 5 to 2.5 wt .-%, contains.
  • a particulate biomass comprising a maltodextrin, in particular a maltodextrin having a DE of from 3 to 10, in an amount of from 0.05 to 6% by weight, in particular from 0.1 to 5% by weight, preferably 0.2 to 4.5 wt .-%, in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt. -%, especially 1, 0 to 3.0 wt .-%, in particular 1, 5 to 2.5 wt .-%, and comprises cells of the genera Thraustochytrium, Schizochytrium, Aurantiochytrium or
  • Oblongichytrium in particular of the species Aurantiochytrium limacinum, especially of the strain Aurantiochytrium limacinum SR21, contains, wherein at least 80 wt .-%, in particular at least 90 wt .-%, preferably at least 95 wt .-%, of the particles contained a particle size of 100 to 2000 microns, preferably 100 to 1500
  • Microns especially 100 to 1000 microns, and further comprising a hydrophilic or hydrophobic silicate in an amount of 0.05 to 6 wt .-%, in particular 0.1 to 5 wt .-%, preferably 0.2 to 4 , 5 wt .-%, in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt .-%, especially 1 , 0 to 3.0 wt .-%, in particular 1, 5 to 2.5 wt .-%, contains.
  • a hydrophilic or hydrophobic silicate in an amount of 0.05 to 6 wt .-%, in particular 0.1 to 5 wt .-%, preferably 0.2 to 4 , 5 wt .-%, in particular 0.4 to 4.0 wt .-%, particularly preferably 0.6 to 3.5 wt .-%, in particular 0.8 to 3.2 wt
  • the particulate biomass according to the invention can be used in different ways. After drying the biomass according to the invention, storage or packaging of the dried biomass is preferably carried out. Subsequently, the biomass can be used, for example, on site to produce a feed or food. A feed or food containing a particulate biomass according to the invention is therefore a further subject of the present invention.
  • Another object of the present invention is therefore also the use of a particulate biomass according to the invention for the production of a food or
  • Another object of the present invention is therefore also a process for the preparation of a feed or food, in which an inventive particulate biomass is used and is preferably mixed with other food or feed ingredients.
  • the particulate biomass may, if appropriate, be subjected to a cell disruption method immediately before the production of the feed or food, as described in the applications WO2014 / 122087 or WO2014 / 122092.
  • biomass can also be processed directly into a feed or foodstuff together with other feed or food components without prior cell disruption.
  • a particulate biomass according to the invention is preferably present in an amount of from 1 to 20% by weight, in particular from 3 to 15% by weight, in a feed or feed according to the invention.
  • the particulate biomass according to the invention is used in a preferred embodiment according to the invention for the production of a food or feed in which the biomass is preferably mixed with other food or feed ingredients and then processed into the food or feed.
  • Extrusion process to obtain ready-to-eat portions of the food or feed.
  • a pelleting process can also be used.
  • a screw or twin-screw extruder is preferably used.
  • the extrusion process is preferably carried out at a temperature of 80 - 220 ° C, especially 100 - 190 ° C, a pressure of 10 - 40 bar, and a Wave circulation speed of 100 - 1000 rpm, in particular 300 - 700 rpm performed.
  • the residence time of the introduced mixture is preferably 5 to 30 seconds, in particular 10 to 20 seconds.
  • this comprises
  • the components are preferably intimately mixed. This is preferably done in a drum equipped with blades.
  • a water vapor injection in particular to effect the swelling of the starch preferably contained.
  • the other food or feed ingredients are preferably comminuted before mixing with the digested cells, if necessary
  • the comminution of the other food or feed ingredients can be done, for example, using a hammer mill.
  • a preferred method for producing a food or feed according to the invention therefore comprises the following steps: a) provision of a particulate biomass which contains a valuable substance, preferably a lipid, more preferably omega-3 fatty acids, preferably by spray granulation of a fermentation broth; b) Granulation of the particulate biomass thus obtained with the addition of a valuable substance, preferably a lipid, more preferably omega-3 fatty acids, preferably by spray granulation of a fermentation broth; b) Granulation of the particulate biomass thus obtained with the addition of a
  • Agglomerationstosmittels wherein as granulation preferably modified cellulose, in particular carboxymethylcellulose, or a maltodextrin, in particular a maltodextrin having a DE value of 3 to 10, is used, so that the
  • Food or feed comprises the following steps: a) provision of a particulate biomass, the slime fungi, in particular those of the family Thraustochytriaceae, especially those of the genera Thraustochytrium,
  • Schizochytrium, Aurantiochytrium or Oblongichytrium contains; b) Granulation of the particulate biomass thus obtained with the addition of a
  • Agglomerationstosmittels wherein as granulation preferably modified cellulose, in particular carboxymethylcellulose, or a maltodextrin, in particular a maltodextrin having a DE value of 3 to 10, is used, so that the
  • Agglomeration aids in the granulation product in an amount of 0.1 to 6 wt .-%, preferably 0.2 to 5 wt .-%, is included; c) mixing of the particulate biomass from (b), optionally after previous
  • Feed ingredients d) Preparation of the final product by a compaction or extrusion process.
  • Preferred methods according to the invention for the production of a foodstuff or feedstuff are preferably characterized in that no higher energy input to the biomass takes place in any process step than 50 kWh per tonne of suspension.
  • the energy input to the biomass is preferably not more than 40 or 35 kWh, in particular not more than 30 or 25 kWh, particularly preferably 20 or 15 kWh, in each case per ton
  • the digested cells preferably make up 0.5-20% by weight, in particular 1-10% by weight, preferably 2-8% by weight, of the foodstuff or feed
  • the food or feed is preferably an aquaculture agent or food or feed for use in poultry, pig breeding or cattle breeding.
  • the feed may also be a feed used to breed micro-organisms that can be used as feed in aquaculture.
  • the microorganisms may be, for example, nematodes, crustaceans or rotifers.
  • the feed is preferably flaked, spherical or tablet-shaped. An available by extrusion
  • Feed preferably has a moisture content of less than 5% by weight, more preferably from 0.2 to 4% by weight.
  • the other food or feed ingredients are preferably selected from proteinaceous, carbohydrate-containing, nucleic acid-containing and lipid-soluble components and optionally further fatty-containing components and furthermore from others
  • Additives such as minerals, vitamins, pigments and amino acids.
  • structuring substances may also be present in order to improve the texture or the appearance of the feedstuff.
  • binders can be used to influence the consistency of the feed.
  • a preferred component that is both a nutrient and a structurant is starch.
  • a protein-containing component which additionally contains fats
  • can be used for example, fish meal, krill flour, shellfish, squid or Schrimpsschalen.
  • fish oil can be used as a fat-containing component.
  • a fat-containing component can also be used a vegetable oil, in particular oil from soybeans, rapeseeds, sunflower seeds and flaxseed.
  • carbohydrate-containing component for example, wheat flour, sunflower flour, soybean meal or
  • Grain gluten can be used.
  • the total content of oil in the feed - including the oil from the oil-containing cells - is preferably 15-50 wt .-%.
  • the feed for use in aquaculture is preferably used to
  • fin fish and crustaceans which are preferably used to nourish humans. These include in particular carp, tilapia, catfish, tuna, salmon, trout, baramundi, bream, perch, cod, shrimp, lobster, crab, shrimp and crayfish. Particularly preferred is a feed for salmon farming.
  • the preferred salmon species are Atlantic salmon, red salmon, masu salmon, king salmon, keta salmon, silver salmon, Danube salmon, pacific salmon and humpback salmon.
  • it may be a feed intended for the cultivation of Fish, which are then processed into fishmeal or fish oil.
  • the fish are preferably herring, pollack, menhaden, anchovies, capelin or cod.
  • the fish meal or fish oil thus obtained can in turn be used in aquaculture for the breeding of food fish or crustaceans.
  • the aquaculture can take place in ponds, tanks, basins or even in delimited areas in the sea or in lakes, in particular in cages or net pens. Aquaculture can be used to grow the ready-made food fish, but can also be used to raise juvenile fish, which are then released to feed the fish
  • a further subject of the present invention is accordingly also a method for breeding animals, in particular fin fish or crustaceans, preferably salmon, in which a feed according to the invention is used.
  • a further subject of the present invention is furthermore an animal, in particular a finfish or shellfish, obtainable by such a method according to the invention.
  • the strain Aurantiochytrium limacinum SR21 was used. This is deposited with NIBH under FERM BP-5034 and IFO under IFO 32693.
  • Strain A. limacinum SR21 was originally isolated from seawater and named Schizochytrium limacinum SR21 (Nakahara et al., 1996, JAOCS, 73 (10); Hyundai Mycol. Res. 1998). Due to reclassification, he was assigned to the new genus Aurantiochytrium and renamed accordingly.
  • the fermentation of the strain was carried out in a medium containing 50% artificial
  • Example 2 Granulation of spray-dried biomass using
  • the granulator ProCell-LabSystem (Glatt, Germany) was used to carry out a fluidized-bed granulation.
  • the GF3 insert was used here.
  • the fine, spray-dried biomass powder was passed into the fluidized bed and sprayed there a carboxymethylcellulose solution (4 wt .-% Blanose, dissolved in water) and thus fine in the
  • the specified conditions corresponded to the standard conditions of a thermal granulation.
  • the product thus obtained showed in comparison to the starting biomass significantly improved product properties, in particular a significantly improved flowability.
  • Example 3 Granulation of spray-dried biomass using maltodextrin DE 3.5 as agglomeration aid
  • the granulator ProCell-LabSystem (Glatt, Germany) was used to carry out a fluidized-bed granulation.
  • the GF3 insert was used here.
  • the fine, spray-dried biomass powder was passed into the fluidized bed where a maltodextrin DE 3.5 solution (25% by weight of maltodextrin DE 3.5, dissolved in water) was sprayed and thus finely distributed in the fluidized bed, so that a final concentration of maltodextrin DE 3.5 of 3% by weight.
  • the solvent was evaporated by heating the supply air to 60 ° C. The supply air velocity was adjusted so that a turbulence of the agglomerated particles is possible.
  • the specified conditions corresponded to the standard conditions of a thermal granulation.
  • the product thus obtained showed in comparison to the starting biomass significantly improved product properties, in particular a significantly improved flowability.
  • Example 4 Granulation of spray-dried biomass using maltodextrin 18.9 as agglomeration aid
  • the granulator ProCell-LabSystem (Glatt, Germany) was used to carry out a fluidized-bed granulation.
  • the GF3 insert was used here.
  • the fine, spray-dried Biomass powder was passed into the fluidized bed and there sprayed a maltodextrin 18.9 solution (40 wt .-% maltodextrin 18.9, dissolved in water) and thus fine in the
  • Fluidized bed distributed so that a final concentration of maltodextrin 18.9 of 3 wt .-% adjusted.
  • the solvent was evaporated by heating the supply air to 60 ° C.
  • the supply air velocity was adjusted so that a turbulence of the agglomerated particles is possible. This was achieved by regulating the supply air to about 80 m 3 / h.
  • the residence time of the particles in the fluidized bed was thus adjusted by the rate of particle growth, since the particles had to grow from the fine, spray-dried particle to the larger agglomerate in order to increase the particle size
  • the specified conditions corresponded to the standard conditions of a thermal granulation.
  • the product thus obtained showed in comparison to the starting biomass significantly improved product properties, in particular a significantly improved flowability.

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Abstract

Selon l'invention, une biomasse particulaire contenant une matière valorisable sensible à l'oxydation peut être transformée avec ménagement en un produit particulièrement facile à manipuler, lorsqu'elle est soumise à une granulation avec adjonction d'un agent auxiliaire d'agglomération.
EP15766527.4A 2014-10-02 2015-09-22 Procédé de fabrication d'une biomasse granulée contenant une matière valorisable sensible à l'oxydation Ceased EP3200611A1 (fr)

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CN106793803B (zh) 2014-10-02 2021-03-09 赢创运营有限公司 通过挤压含pufa的生物质来制备含pufa的饲料的方法
CA2958457C (fr) 2014-10-02 2022-10-25 Evonik Industries Ag Procede de production d'une biomasse contenant des agpi qui presente une haute stabilite cellulaire
US11464244B2 (en) 2014-10-02 2022-10-11 Evonik Operations Gmbh Feedstuff of high abrasion resistance and good stability in water, containing PUFAs
EP3200604B1 (fr) 2014-10-02 2021-11-03 Evonik Operations GmbH Procédé pour la préparation d'un aliment pour animaux
WO2018011275A1 (fr) 2016-07-13 2018-01-18 Evonik Degussa Gmbh Procédé d'isolement de lipides à partir de cellules contenant des lipides
US11946017B2 (en) 2016-07-13 2024-04-02 Evonik Operations Gmbh Method of separating lipids from a lysed lipids containing biomass
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US11352651B2 (en) 2016-12-27 2022-06-07 Evonik Operations Gmbh Method of isolating lipids from a lipids containing biomass
WO2018122057A1 (fr) 2016-12-27 2018-07-05 Evonik Degussa Gmbh Procédé d'isolement de lipides à partir d'une biomasse contenant des lipides
CN111356767A (zh) 2017-08-17 2020-06-30 赢创运营有限公司 通过限制至少两种限制性营养源增强脂质的产生
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BR112017006835A2 (pt) 2018-06-19
CL2017000753A1 (es) 2017-12-15
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CN106793802A (zh) 2017-05-31
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