EP3144315A4 - Composé fluorescent spécifique à une enzyme, pouvant être retenu dans des cellules - Google Patents

Composé fluorescent spécifique à une enzyme, pouvant être retenu dans des cellules Download PDF

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Publication number
EP3144315A4
EP3144315A4 EP15793236.9A EP15793236A EP3144315A4 EP 3144315 A4 EP3144315 A4 EP 3144315A4 EP 15793236 A EP15793236 A EP 15793236A EP 3144315 A4 EP3144315 A4 EP 3144315A4
Authority
EP
European Patent Office
Prior art keywords
enzyme
retained
cells
compound capable
fluorescent compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP15793236.9A
Other languages
German (de)
English (en)
Other versions
EP3144315B1 (fr
EP3144315A1 (fr
Inventor
Yasuteru Urano
Mako Kamiya
Tomohiro Doura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Tokyo NUC
Original Assignee
University of Tokyo NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by University of Tokyo NUC filed Critical University of Tokyo NUC
Publication of EP3144315A1 publication Critical patent/EP3144315A1/fr
Publication of EP3144315A4 publication Critical patent/EP3144315A4/fr
Application granted granted Critical
Publication of EP3144315B1 publication Critical patent/EP3144315B1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
  • Pyrane Compounds (AREA)
EP15793236.9A 2014-05-14 2015-05-13 Composé fluorescent spécifique à une enzyme, pouvant être retenu dans des cellules Active EP3144315B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2014100771 2014-05-14
PCT/JP2015/063789 WO2015174460A1 (fr) 2014-05-14 2015-05-13 Composé fluorescent spécifique à une enzyme, pouvant être retenu dans des cellules

Publications (3)

Publication Number Publication Date
EP3144315A1 EP3144315A1 (fr) 2017-03-22
EP3144315A4 true EP3144315A4 (fr) 2017-12-13
EP3144315B1 EP3144315B1 (fr) 2022-07-06

Family

ID=54479997

Family Applications (1)

Application Number Title Priority Date Filing Date
EP15793236.9A Active EP3144315B1 (fr) 2014-05-14 2015-05-13 Composé fluorescent spécifique à une enzyme, pouvant être retenu dans des cellules

Country Status (6)

Country Link
US (1) US9981934B2 (fr)
EP (1) EP3144315B1 (fr)
JP (1) JP6635555B2 (fr)
CN (1) CN106459125B (fr)
CA (1) CA2948306A1 (fr)
WO (1) WO2015174460A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3275889A4 (fr) * 2015-02-27 2018-11-21 The University of Tokyo Sonde fluorescente pour détecter l'activité de la calpaïne
JP6849983B2 (ja) * 2016-06-30 2021-03-31 国立大学法人 東京大学 酵素特異的な細胞内滞留性赤色蛍光プローブ。
CN106632215B (zh) * 2016-11-17 2019-04-12 陕西师范大学 一种荧光蛋白质染色剂及其制备方法和应用
CN109694396A (zh) * 2018-12-26 2019-04-30 济南大学 一种双光子比率荧光探针在检测β-半乳糖苷酶中的应用
CN109651326B (zh) * 2019-01-08 2021-06-01 厦门大学 一类共价键连接标记细胞的荧光探针和跟踪标记细胞的方法
CN113474342A (zh) * 2019-02-28 2021-10-01 国立大学法人东京大学 检测癌症的荧光探针
CN111773394A (zh) * 2019-04-04 2020-10-16 复旦大学 一种β-半乳糖苷酶荧光探针纳米微球及其制备方法和用途
JP2023012556A (ja) * 2019-06-14 2023-01-26 株式会社同仁化学研究所 細胞滞留性蛍光化合物並びにそれを用いた細胞の染色方法及び高感度検出方法
WO2022173576A1 (fr) * 2021-02-10 2022-08-18 The Regents Of The University Of California Stratégie tandem de détection et de marquage basée sur l'activité pour l'imagerie d'espèces réactives de l'oxygène

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014065673A (ja) * 2012-09-25 2014-04-17 Univ Of Tokyo 標的細胞特異的な光増感用化合物。

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO884565L (no) * 1987-10-15 1989-04-17 Univ Moskovsk Preparat for kontrastdannelse ved diagnose av ondartet neoplasma.
US7868147B2 (en) 2003-09-05 2011-01-11 Tetsuo Nagano Fluorescent probe
US20080014147A1 (en) * 2006-02-28 2008-01-17 The University Of Tokyo Tumor specific fluorescent dye
US8143069B2 (en) 2006-03-03 2012-03-27 The University Of Tokyo Fluorescent probe and method of measuring hypochlorite ion
US8461358B2 (en) 2009-02-20 2013-06-11 The University Of Tokyo Fluorescent probe for measuring protease
WO2011087000A1 (fr) 2010-01-13 2011-07-21 国立大学法人 東京大学 Diagnostic pour le cancer
WO2011149109A1 (fr) * 2010-05-25 2011-12-01 三菱レイヨン株式会社 Substrat fluorescent pour la détection de l'activité enzymatique d'une enzyme agissant sur les nitriles
EP2857830B1 (fr) * 2012-05-30 2021-08-25 The University of Tokyo Sonde fluorescente pour la détection haute sensibilité de liquide pancréatique et procédé de détection de liquide pancréatique

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014065673A (ja) * 2012-09-25 2014-04-17 Univ Of Tokyo 標的細胞特異的な光増感用化合物。

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MAKO KAMIYA ET AL: "[beta]-Galactosidase Fluorescence Probe with Improved Cellular Accumulation Based on a Spirocyclized Rhodol Scaffold", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 133, no. 33, 24 August 2011 (2011-08-24), US, pages 12960 - 12963, XP055236721, ISSN: 0002-7863, DOI: 10.1021/ja204781t *
See also references of WO2015174460A1 *

Also Published As

Publication number Publication date
US20170073321A1 (en) 2017-03-16
EP3144315B1 (fr) 2022-07-06
WO2015174460A1 (fr) 2015-11-19
JP6635555B2 (ja) 2020-01-29
CA2948306A1 (fr) 2015-11-19
CN106459125A (zh) 2017-02-22
JPWO2015174460A1 (ja) 2017-04-20
CN106459125B (zh) 2019-05-07
EP3144315A1 (fr) 2017-03-22
US9981934B2 (en) 2018-05-29

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