EP3063572A1 - Guide de lumière creux pourvu d'orifices, en particulier pour alimenter un photobioréacteur en lumière et en substances nutritives - Google Patents

Guide de lumière creux pourvu d'orifices, en particulier pour alimenter un photobioréacteur en lumière et en substances nutritives

Info

Publication number
EP3063572A1
EP3063572A1 EP14793005.1A EP14793005A EP3063572A1 EP 3063572 A1 EP3063572 A1 EP 3063572A1 EP 14793005 A EP14793005 A EP 14793005A EP 3063572 A1 EP3063572 A1 EP 3063572A1
Authority
EP
European Patent Office
Prior art keywords
light
light guide
photobioreactor
hollow core
nutrients
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14793005.1A
Other languages
German (de)
English (en)
Inventor
Robert Schreiber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Airbus Defence and Space GmbH
Original Assignee
Airbus Defence and Space GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Airbus Defence and Space GmbH filed Critical Airbus Defence and Space GmbH
Publication of EP3063572A1 publication Critical patent/EP3063572A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus
    • C12M43/08—Bioreactors or fermenters combined with devices or plants for production of electricity
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/0001—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems
    • G02B6/0005—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems the light guides being of the fibre type
    • G02B6/001—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems the light guides being of the fibre type the light being emitted along at least a portion of the lateral surface of the fibre
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/0001—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems
    • G02B6/0096—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems the lights guides being of the hollow type

Definitions

  • Hollow light guide with openings in particular for supplying a photobioreactor with light and nutrients
  • the present invention relates to a specially designed hollow light guide and a light guide assembly. Furthermore, the invention relates to a photobioreactor, in which such a light guide or such a light guide arrangement is used. Moreover, the invention relates to a method for operating a photobioreactor and to a method for manufacturing a light conductor that can be used advantageously in a photobioreactor.
  • Phototrophic organisms are microorganisms, e.g. in the form of microorganisms that can directly use light as an energy source for their metabolism. Phototrophic organisms include, for example, certain plants, mosses, microalgae, macroalgae, cyanobacteria and purple bacteria.
  • biomass for example in the form of algae in large quantities and at low cost.
  • biomass may be used to produce alternative biofuels, e.g. used for the transport sector.
  • a bioreactor In order to produce biomass on an industrial scale, so-called bioreactors are used.
  • a bioreactor is a plant for the production of organisms outside their natural and within an artificial technical environment.
  • So-called photobioreactors are used to phototrophic To cultivate organisms.
  • a photobioreactor provides the phototrophic organisms with both light and nutrients, for example CO 2, as well as a suitable nutrient solution, so that they can build up biomass accordingly.
  • open photobioreactor systems sometimes referred to as open ponds
  • phototrophic organisms are grown in open tanks or ponds in a controlled manner.
  • a nutrient solution or culture suspension containing all the nutrients and CO 2 necessary for the particular organism is usually conveyed in a cycle and, from the open surface, mostly illuminated directly by the sun.
  • closed photobioreactor systems have been developed.
  • a nutrient solution is conducted together with the organisms through a closed circuit and is usually illuminated from the outside.
  • a tube photobioreactor glass or plastic tubes are assembled into a closed circuit and the organisms enclosed therein are supplied with nutrients and CO 2 by means of a central unit, which may for example contain suitable pumps and sensors.
  • Closed photobioreactors usually allow a high process control, as the organisms and the surrounding nutrient solution in the closed System can be well heated or cooled, monitors a pH and can be adjusted if necessary and additional light can be provided.
  • the closed systems allow for low space requirement high productivity, since for example several closed systems can be arranged one above the other or tubes of a system can run in the vertical direction and can be illuminated from all sides. However, shadowing effects are always to be expected.
  • a high product purity with low contamination, low evaporation and low electromagnetic interference (EMC) are possible.
  • a specially designed elongate hollow light guide is proposed.
  • the light guide has a sheath of a transparent material surrounding a hollow core.
  • the hollow core in this case has a diameter of at least 1 mm, preferably at least 3 mm or at least 1 cm.
  • an optical fiber assembly comprising a plurality of optical fibers according to the above first aspect.
  • a photobioreactor comprising a container for containing organisms in a solution and a light guide or optical fiber assembly according to the above first or second aspect for supplying the organisms in the container with light and nutrients.
  • a method for operating such a photobioreactor comprises introducing light via the light guide into the solubilized solution as well as an introduction of nutrients through the hollow core of the light guide into the solubilized solution.
  • a method for manufacturing a light guide according to the above first aspect is proposed.
  • Phototrophic organisms should be well supplied with light as well as nutrients during their rearing.
  • light for example by means of optical fibers
  • the phototrophic organisms are contained in a nutrient solution.
  • these nutrients are conventionally introduced via various tributaries in the nutrient solution inside the container.
  • gases such as CO 2
  • water and trace elements are each introduced via separate tributaries. This generally requires a complex construction of the photobioreactor as well as the various nutrient sources coupled to it.
  • optical fibers have been used primarily to guide light from one location to another as lossless as possible.
  • the light guides are used for example in the form of glass fibers mostly for data transmission.
  • As a material for such optical fiber usually highly transparent glass is used.
  • the light guide is generally made as a thin fiber with an outer diameter of usually well below 1 mm.
  • hollow fibers known, as used for example in the textile industry as insulating material or absorbent filling material or for the construction of filter modules.
  • the sheath of such hollow fibers need not be made of a transparent material.
  • a light guide as a kind of hollow fiber, in which on the one hand the sheath consists of a transparent material and on the other hand, the surrounded by the sheath hollow core sufficient
  • the core should have a diameter of at least 1 mm, preferably at least 3 mm or better at least 1 cm, so that flowable nutrients can be transported through this hollow core without excessive friction losses.
  • a plurality of openings are provided in the mantle of the hollow light guide. About these openings is the hollow core of the light guide in contact with the environment. A diameter of these openings should be at least 0.5 mm, preferably at least 1 mm, so that nutrients delivered by the hollow core can flow easily and with as little friction as possible through the openings to the outside into the container.
  • the proposed hollow light guide can thus serve advantageously to conduct both light and nutrients from one place to another or promote.
  • the transparent jacket of the light guide mainly serves to guide the light. It also encloses the hollow core, through which the nutrients can be promoted. By virtue of the openings formed in the shell at desired locations, the substances conveyed in the hollow core can then escape to the outside.
  • such hollow optical fibers can therefore be advantageously used to introduce from outside both light and nutrients into the interior of a container of the photobioreactor.
  • the jacket of the light guide is made of plastic.
  • plastic for the jacket can also ensure the required large diameters of the proposed light guide for sufficient mechanical strength and / or sufficient mechanical elasticity.
  • any plastic or any plastic mixture can be used, which or has a sufficient for the intended use optical transparency with suitable mechanical properties.
  • PMMA polymethyl methacrylate
  • POF light guide in English: "polymer optical fiber”
  • the sheath may consist of a material which can be heated without damage to at least 60 ° C. This can be achieved that the optical fiber can be thermally sterilized. This may be particularly important for use in photobioreactors.
  • the jacket is closed on at least one end face.
  • This advantage can also be achieved if the light guide in the region of the end face is solid, that is not hollow.
  • the massively formed area extends from the end face to 1 mm, 1 cm, 20 cm or up to 50 cm in length of the light guide.
  • Another advantage of the closed end face is that the escape of the gases and / or nutrients to be introduced can take place from the openings provided, for example. The introduction of the gas and / or the nutrients can be controlled, for example by the number of openings per length of the light guide.
  • the optical waveguides have different outer diameters.
  • the different hollow optical fibers have different inner diameters, that is, hollow cores of different diameters.
  • the different light guides can be adapted to different uses. For example, an optical fiber with a smaller outer diameter and thus a smaller hollow core can be well used to promote gaseous nutrients such as CO2, since friction losses generally play a minor role here.
  • the optical fibers can be combined into a bundle.
  • Several light guides can be mechanically connected to each other, for example by means of an adhesive or connecting fibers, and thus, for example, be handled better.
  • the light guides may be woven into a mat.
  • the optical fibers can be mechanically sufficiently firmly connected to each other without further aids such as adhesives or connecting fibers.
  • the proposed hollow light guide may have further advantageous features.
  • the jacket may be provided with an additional layer on its inner surface facing the hollow core.
  • this additional layer may provide chemical resistance to hollow core passed media.
  • the additional layer may also serve as a reflective layer to prevent light conducted in the cladding leaving the cladding sideways toward the hollow core and then being absorbed in the medium conveyed in the hollow core, for example, and thus lost for light supply of the phototrophic organisms goes.
  • the additional layer may be metallic, for example.
  • the jacket of the hollow optical waveguide can also be selectively designed such that light conducted in the cladding does not exit exclusively at the end faces of the optical waveguide, but a significant portion of this light is already coupled out laterally transversely to the waveguide to the outside.
  • light is coupled not only locally and thus with high intensity at the end of the light guide into the solution receiving the organisms, but over a larger area along, for example, the entire mantle surface of the light guide the solution is irradiated with light.
  • the outer surface of the shell can be selectively roughened to provoke a lateral decoupling of light from the light guide.
  • the hollow light guide or the light guide arrangement, as described above, can be advantageously used, in particular for a photobioreactor, to supply light and nutrients in this organism.
  • the photobioreactor may have a light source which is connected to at least one of the light guides so that light from the light source can be coupled into the light guide and transferred via the light guide into the solution containing organisms.
  • the photobioreactor may further comprise a nutrient source connected to at least one of the light guides such that nutrients may be introduced from the nutrient source into the hollow core of the light pipe and transferred thereinto into the organism-added solution.
  • the photobioreactor may comprise a plurality of different nutrient sources and a plurality of different light guides, each of the nutrient sources configured to provide a liquid or gaseous nutrient and connected to at least one of the light guides to deliver nutrients from the nutrient source to the hollow Inserted core of the light guide and can be transferred via this in the organisms mixed with solution.
  • each of the nutrient sources configured to provide a liquid or gaseous nutrient and connected to at least one of the light guides to deliver nutrients from the nutrient source to the hollow Inserted core of the light guide and can be transferred via this in the organisms mixed with solution.
  • another light guide with different properties can be used.
  • a hollow optical fiber having a thinner core diameter may be used than for transferring nutrients received in a liquid solution.
  • the photobioreactor may further comprise a suction device, which is designed and connected to at least one of the light guides so that it can be sucked with the aid of organisms displaced solution from the container through the hollow core of the light guide therethrough and then for a Analysis of the solubilized solution can be provided.
  • the hollow light guide can not only be provided to introduce light and nutrients into the container of the photobioreactor, but also in the opposite direction can be removed from the container via the hollow light guide, for example, to analyze them with organisms displaced nutrient solution.
  • Fig. 1 shows a cross-sectional view of a hollow optical fiber according to an embodiment of the present invention.
  • Fig. 2 shows a perspective view of a hollow light guide according to an embodiment of the present invention.
  • FIG. 3 is a perspective view of an optical fiber assembly according to an embodiment of the present invention.
  • FIG. 4 shows a perspective view of a woven light guide assembly according to another embodiment of the present invention.
  • Fig. 5 shows a photobioreactor according to an embodiment of the present invention.
  • the light guide 1 has a jacket 3 made of transparent plastic, which encloses a hollow core 5.
  • the jacket 3 is cylindrical and has an outside diameter D a of, for example, 1 cm.
  • An inner diameter D, of the shell 3 corresponds to the diameter of the hollow core 5 and is for example 0.5 cm.
  • a plurality of through openings 7 are formed at least in one subregion, via which the hollow core 5 is connected to the circumference. tion and in particular can exchange fluids.
  • the openings 7 have, for example, a diameter d of at least 0.5 mm.
  • the openings 7 are arranged spaced apart from each other in the shell, wherein a distance between adjacent openings 7 is preferably significantly greater than the diameter d of the openings 7 in order not to unduly weaken the stability of the light guide 1.
  • the jacket 3 of the optical waveguide 1 is closed on an end face (in FIG. 1 on the right-hand side).
  • the light guide 1 can be used, for example, in a photobioreactor in order to be able to introduce both light 13 and nutrients 15 from the outside into a container in the interior of the photobioreactor.
  • the light 13 is thereby coupled into the light guide 1, in particular in its jacket 3, coming from a light source and can then propagate along the light guide 1, similar to conventional light guides.
  • the optical waveguide 1 is preferably adapted specifically for the fact that the light 13 is not completely propagated through the optical waveguide 1 from one end to the other end, but that it is successively coupled out of the cladding 3 along the optical waveguide 1, preferably continuously outwards indicated by the arrows 19 in Fig. 1.
  • the light 13 thus need not be coupled out locally at the end of the light guide 1, but can be coupled along the entire outer surface of the jacket 3 and thus illuminate the solution contained in the container of the photobioreactor containing organisms solution over a large area.
  • the openings 7 are also distributed evenly over the jacket 3 of the light guide 1, so that the nutrients 15 transferred in the hollow core 5 are preferably uniform along the length of the light guide 1 through the plurality of openings 7 can escape to the outside and the organisms contained in the surrounding solution can be supplied.
  • the light guide assembly 21 has a bundle 9 of a plurality of optical fibers 1.
  • the light guides 1 have different outer diameters.
  • gases such as, for example, CO.sub.2 can be conducted through cores 5 of diameter-smaller light guides 1, whereas liquids can also be conducted through the hollow core 5 of a light conductor 1 with a larger diameter. Both the gases and the liquids can escape through the openings 7 to the outside into a nutrient solution present, for example, in the photobioreactor.
  • Fig. 4 shows an alternative embodiment of an optical fiber assembly 21, in which a plurality of optical fibers 1 are woven into a mat 1 1.
  • the same or different types of optical fibers 1 can be used here, wherein the optical fibers 1 can differ in particular with respect to their diameter.
  • the optical fibers 1 in areas where the optical fibers 1 within the fabric 1 1 are most curved, it may come to the local coupling of guided in the optical fibers 1 light, as indicated in turn by the arrows 19, so that light preferably over the entire mat. 1 1 back and forth to the surface can be coupled out.
  • a nutrient solution in a photobioreactor can be illuminated over a large area.
  • FIG. 5 schematically illustrates a photobioreactor 100 according to an embodiment of the present invention.
  • the photobioreactor 100 has a container 23 in which phototrophic organisms can be taken up in a solution 25.
  • a plurality of optical fibers 1 are included in order to provide the phototrophic organisms with both light and nutrients.
  • the photobioreactor 1 further has at least one light source 27 and at least one nutrient source 45, 47.
  • the light source 27 may include one or more components for artificially generating light or collecting naturally generated light, and then coupling that light into a common light guide 1 to power the bioreactor 100.
  • the light source 27 can be configured as a light source 29 for collecting and coupling sunlight into the light guide.
  • the light source 29 may be configured, for example, as a solar collector 30 with a concave mirror, which focuses sunlight on a receiver.
  • the light source 27 may be formed as an artificial light source 31 in which, for example, by means of an LED 32 or a laser 33 light is generated, which is then irradiated to an array 35 of a polarizer and a shading, which in turn with the light guide.
  • the artificial light source 31 is connected to the container 23 of the photobioreactor 1.
  • the artificial light source 31 can be supplied, for example, with electric current from alternative sources such as wind power 39 or solar cells 41 or alternatively by conventional current 43.
  • the electric current can be temporarily stored, for example, via a buffer battery 37, so that the artificial light source 31 can expose the photobioreactor 100 even in the absence of sunshine.
  • the optical fibers 1 are not only used to transfer light received by the light source 27 into the interior of the container 23 and the organism-containing solution 25 contained therein.
  • nutrient sources 45, 47 are connected to the light guides 1 and in particular to their hollow core 5.
  • nutrient sources 45, 47 for example, CO2 and another nutrient-containing nutrient solution are fed into the interior of the hollow optical fibers 1 and then transferred via the hollow cores 5 into the container 23. There, the nutrients via the openings 7 escape from the hollow light guides 1.
  • the hollow optical fibers 1 can also be used to suck solution 25 containing organisms via a suction device 49 out of the container 23 so that it can be analyzed, for example.
  • the solution 25 is sucked through the openings 7 in the optical fibers 1 in the core 5 and then pumped out of the interior of the container 23 out, for example, to an analysis device.
  • an agitator 51 is also provided, by means of which the solution 25 can be permanently circulated.
  • an optical waveguide in which a cladding 3 of transparent material surrounds a hollow core 5 can be provided.
  • the hollow core 5 should preferably have a diameter of at least 1 mm, more preferably at least 3 mm or 1 cm.
  • a plurality of openings 7 can be generated in the jacket 3 of the light guide 1.
  • the jacket can be locally irradiated with a laser of sufficient power density, so that material of the jacket 3 is locally removed and the openings 7 are formed.
  • a diameter and a power of the laser used for this purpose can be suitably chosen such that openings form with a diameter of at least 0.5 mm. If the optical waveguide 1 is already hollow in this processing stage, then two openings 7 can be produced simultaneously in opposite regions of the cladding 3 with a single laser irradiation.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un guide de lumière (1) creux allongé, utilisable en particulier dans un photobioréacteur pour fournir de la lumière ainsi que des substances nutritives à des organismes phototrophes. Le guide de lumière (1) comporte une enveloppe (3) en matériau transparent qui entoure une âme creuse (5). L'âme creuse possède un diamètre au moins égal à 1 mm, de préférence au moins égal à 3 mm ou 1 cm. L'enveloppe (3) est percée d'une pluralité d'orifices (7) de diamètre au moins égal à 0,5 mm, de préférence au moins égal à 1 mm. La lumière peut se propager à travers l'enveloppe transparente et sortir de préférence latéralement (19) sur toute la longueur du guide de lumière (1). Des substances nutritives (15) peuvent être introduites à l'intérieur du photobioréacteur par le biais de l'âme creuse (5). Inversement, des fractions de la solution mélangée à des organismes peuvent aussi être aspirées à travers l'âme creuse (5), par exemple en vue de les analyser.
EP14793005.1A 2013-10-28 2014-10-14 Guide de lumière creux pourvu d'orifices, en particulier pour alimenter un photobioréacteur en lumière et en substances nutritives Withdrawn EP3063572A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE201310017742 DE102013017742A1 (de) 2013-10-28 2013-10-28 Hohler Lichtleiter mit Öffnungen, insbesondere zur Versorgung eines Photobioreaktors mit Licht und Nährstoffen
PCT/DE2014/000506 WO2015062563A1 (fr) 2013-10-28 2014-10-14 Guide de lumière creux pourvu d'orifices, en particulier pour alimenter un photobioréacteur en lumière et en substances nutritives

Publications (1)

Publication Number Publication Date
EP3063572A1 true EP3063572A1 (fr) 2016-09-07

Family

ID=51846430

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14793005.1A Withdrawn EP3063572A1 (fr) 2013-10-28 2014-10-14 Guide de lumière creux pourvu d'orifices, en particulier pour alimenter un photobioréacteur en lumière et en substances nutritives

Country Status (6)

Country Link
US (1) US9851485B2 (fr)
EP (1) EP3063572A1 (fr)
CN (1) CN105899652B (fr)
AU (1) AU2014344323B2 (fr)
DE (1) DE102013017742A1 (fr)
WO (1) WO2015062563A1 (fr)

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WO2021129923A1 (fr) * 2019-12-23 2021-07-01 Marigan Ag Dispositif pour la culture de microorganismes photosynthétiques

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Also Published As

Publication number Publication date
WO2015062563A1 (fr) 2015-05-07
DE102013017742A1 (de) 2015-04-30
CN105899652B (zh) 2018-03-13
US9851485B2 (en) 2017-12-26
US20160349426A1 (en) 2016-12-01
CN105899652A (zh) 2016-08-24
AU2014344323B2 (en) 2017-12-07
AU2014344323A1 (en) 2016-06-16

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