WO2021129923A1 - Dispositif pour la culture de microorganismes photosynthétiques - Google Patents

Dispositif pour la culture de microorganismes photosynthétiques Download PDF

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Publication number
WO2021129923A1
WO2021129923A1 PCT/EP2019/086932 EP2019086932W WO2021129923A1 WO 2021129923 A1 WO2021129923 A1 WO 2021129923A1 EP 2019086932 W EP2019086932 W EP 2019086932W WO 2021129923 A1 WO2021129923 A1 WO 2021129923A1
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WO
WIPO (PCT)
Prior art keywords
diaphragm
areas
suspension
light
receiving cavity
Prior art date
Application number
PCT/EP2019/086932
Other languages
German (de)
English (en)
Inventor
Markus Amann
Original Assignee
Marigan Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Marigan Ag filed Critical Marigan Ag
Priority to PCT/EP2019/086932 priority Critical patent/WO2021129923A1/fr
Publication of WO2021129923A1 publication Critical patent/WO2021129923A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure

Definitions

  • the present invention relates to a device for cultivating photosynthesis operating microorganisms, the device having at least one reactor vessel and the reactor vessel having at least one light-permeable wall and a receiving cavity for receiving a suspension with the microorganisms to be cultivated, the translucent wall delimiting the receiving cavity at least in some areas .
  • the invention also relates to a method for cultivating photosynthesis operating microorganisms with such a device.
  • Photosynthesizing microorganisms such as microalgae, diatoms, cyanobacteria or archaea use sunlight and carbon dioxide to generate biochemical energy in the form of chlorophyll, lipids, proteins and other substances, which they store within their cell structure.
  • Numerous species of these microscopic microorganisms live in fresh and salt water and, under stressful conditions, generate high-quality and unique substances that enable them to survive under the most difficult environmental conditions.
  • Dr. Rosa Maria Sastre carried out a series of tests with flashlight in the laboratory in order to provide high-frequency light-dark cycles for the cultivation of microorganisms.
  • the object of the invention is therefore to provide devices of the type mentioned in the introduction with which an increased yield can be ensured in the cultivation of photosynthesizing microorganisms.
  • the invention provides for this in a device of initially mentioned that the device has at least one screen with a sequence of light-permeable screen areas and opaque screen areas arranged adjacent thereto and the screen is arranged on the light-permeable wall, and that the device has at least one drive for generating a relative movement between the screen and the suspension received in the receiving cavity.
  • the drive is suitable for to move the diaphragm and the suspension relative to one another in such a way that a large number of partial volumes of the suspension in the receiving cavity are exposed to light alternately in an illumination period through one of the translucent diaphragm areas and shaded in an immediately following shading period by means of one of the opaque diaphragm areas in each case become.
  • An optimum for the cultivation of photosynthetic microorganisms is achieved when the duration of the lighting periods and / or the shading periods is in a value range from 4 milliseconds to 20 milliseconds, preferably from 4 milliseconds to 10 milliseconds.
  • cultivation of photosynthetic microorganisms describes both the growth of the microorganisms and their reproduction, in particular through cell division.
  • devices according to the invention and also the method according to the invention described below it is possible to cultivate primarily photochromic microalgae, i.e. microalgae operating photosynthesis, but also other photochromic microorganisms such as the diatoms, cyanobacteria and / or archaea already mentioned above.
  • the receiving cavity of the reactor vessel in which the suspension is located could also be referred to as the receiving space.
  • the reactor vessels of the device according to the invention could also be referred to as photobioreactors.
  • the suspension with the microorganisms to be cultivated has not only the microorganisms and a liquid, in particular in the form of water, but also that required for photosynthesis Carbon dioxide and optionally other components promoting the cultivation of the microorganisms.
  • These can be nutrients, trace elements and / or vitamins, for example.
  • the composition and concentration of these substances can be tailored to the specific needs of the respective species.
  • the light penetrating through the diaphragm into the receiving cavity of the reactor vessel can be both sunlight and artificial light. It can be directly irradiated light but also scattered light.
  • the temperature in the suspension can optionally be adjusted to the optimum ranges for the cultivation of the respective microorganisms by means of appropriate heating and / or cooling means.
  • the screen can also consist of thermally insulating material in order to help ensure the optimum temperature in the suspension.
  • the drive can be designed very differently. It can be provided, for example, that the drive is a device for generating a flow movement of the suspension through the receiving cavity, preferably a pump. In this case, the diaphragm can be fixedly attached to the reactor vessel. The relative movement between the suspension and the diaphragm is achieved by the flow movement of the suspension generated by the drive. As an alternative to this, however, it can also be provided that the drive is a diaphragm drive for moving the diaphragm relative to the translucent wall of the reactor vessel. In this case, the suspension can largely rest in the receiving cavity of the reactor vessel. In this case, the relative movement is generated by the movement of the diaphragm. Of course are too Mixed forms are conceivable in which both the suspension and the diaphragm are moved relative to the reactor vessel.
  • the transparent aperture areas can be designed, for example, as holes in the aperture or the like. Particularly preferred variants, however, provide that the translucent screen areas are designed as slots in the screen and the opaque screen areas arranged adjacent thereto are designed as webs of the screen.
  • the diaphragm In order to ensure as clear a demarcation as possible between the shaded areas produced by means of the diaphragm in the suspension or in the receiving cavity and the illuminated areas, it is advantageous to design the diaphragm as light-focussing as possible.
  • a first measure for this can be that the light-permeable aperture areas of the aperture are each designed as a light-focussing, preferably depth-extending, light guide channel in the aperture. The depth of the light guide channel reduces the light scattering effects on the light exit opening of the light guide channel facing the receiving cavity, so that a clear delimitation of the shaded areas from the illuminated areas is achieved.
  • the or a depth extension of the respective light-focusing light guide channel measured from a diaphragm surface of the diaphragm facing away from the receiving cavity to a diaphragm surface of the diaphragm facing the receiving cavity, in a value range from 6mm (millimeters) to 15mm, preferably from 9mm to 11mm, lies.
  • the respective light-focusing light guide channel apart from one light inlet opening of the light-focusing light guide channel and one
  • Light exit opening of the light-focusing light guide channel is delimited by light-absorbing channel walls of the diaphragm. Due to the light-absorbing design of the channel walls delimiting the respective light guide channel of the diaphragm, light reflections on the channel walls are avoided, which additionally improves the focusing of the light emerging from the light exit opening of the light guide channel.
  • the channel walls can be made matt black, for example.
  • all other walls of the reactor vessel that delimit the receiving cavity are designed to be light-impermeable and / or light-absorbing.
  • the photosynthesis operating microorganisms generate under stress conditions high-quality and unique substances that are particularly valuable for the manufacture of pharmaceutical products, cosmetics and / or food supplements. These stress conditions are created, among other things, by high light input into the suspension.
  • preferred variants of the invention provide that the diaphragm (s) is or can be releasably fastened or fastened to the reactor vessel by means of a releasable fastening device from the translucent wall and can be removed manually and / or mechanically from the reactor vessel and on it is or are re-attachable.
  • a screen surface of the screen facing away from the receiving cavity is made reflective.
  • the light which does not penetrate through the transparent aperture areas into the receiving cavity and thus into the suspension is reflected or mirrored, so that it is available for illuminating adjacent devices according to the invention.
  • a high light density can be generated between the devices arranged adjacent to one another and the incident light can thus be optimally used.
  • the reactor vessel is designed as a flat-bed reactor vessel in which two opposing, flat and parallel walls and preferably opaque end walls arranged between them delimit the receiving cavity.
  • Such flat-bed reactor vessels could also be referred to as flat-plate reactor vessels.
  • Various embodiments according to the invention can also be implemented with these flat-bed reactor vessels.
  • both of the opposing walls, which are flat and parallel to one another are each designed as a translucent wall and a panel with a sequence of translucent panel areas and opaque panel areas adjacent to them is arranged on each of these walls.
  • the opposing, flat and parallel arranged walls is designed as a light-permeable wall and a screen with a sequence of light-permeable screen areas and opaque screen areas arranged adjacent to them is arranged on this wall and the other is the opposing, planar and parallel to each other arranged walls is opaque and / or light-absorbing.
  • Preferred variants of devices according to the invention with reactor vessels in the form of flat-bed reactor vessels provide that the receiving cavity is subdivided into meandering flow channels for the suspension by means of preferably opaque and / or light-absorbing partitions.
  • the light-permeable screen areas which are designed in particular in the form of slots favorably extend over at least 50%, preferably over at least 90% of the width of the respective flow channel measured orthogonally to the flow direction in the flow channel. In this way it can be achieved that correspondingly shaded areas and illuminated areas are formed in the receiving cavity and thus in the suspension over the entire width of the respective flow channel.
  • the suspension should flow through the flow channels as free as possible of negative shear forces.
  • preferred variants of the invention provide that the meandering flow channels have the same flow cross section everywhere.
  • the receiving cavity is designed as an inherently ring-shaped, in particular circular, closed volume, which is surrounded by an inherently ring-shaped, in particular circular, outer wall of the reactor vessel and by an in annular, in particular circular, inner wall of the reactor vessel and by a bottom wall of the reactor vessel and a top wall of the reactor vessel is limited, at least one of the walls selected from the group consisting of the outer wall, the inner wall, the bottom wall and the top wall, as the translucent wall is formed and the screen is arranged on this translucent wall.
  • the suspension in this ring-shaped receiving cavity in a Flow movement is offset in order to bring about the relative movement between the suspension and the diaphragm.
  • the drive is a diaphragm drive for rotating the diaphragm relative to the translucent wall, preferably around the translucent wall, of the reactor vessel.
  • both the suspension in the annular receiving cavity and the diaphragm are set in motion.
  • the invention also relates to a method for cultivating photosynthetic microorganisms with a device according to the invention.
  • a suspension with the microorganisms to be cultivated in the receiving cavity is exposed to light in some areas through the translucent diaphragm areas and shaded against incidence of light in some areas by the opaque diaphragm areas and the diaphragm and the suspension in the receiving cavity are moved relative to one another by means of the drive .
  • This method is preferably designed in such a way that the diaphragm and the suspension are moved relative to one another by means of the drive in such a way that a large number of partial volumes of the suspension in the receiving cavity are exposed to light alternately in a lighting period through one of the translucent diaphragm areas and in one immediately following shading period can be shaded by means of one of the opaque aperture areas.
  • the duration of the lighting periods and / or the In this case, shading periods are advantageously in a value range from 4 milliseconds to 20 milliseconds, preferably from 4 milliseconds to 10 milliseconds.
  • FIG. 1 to 5 are schematic representations relating to a first exemplary embodiment of a device according to the invention.
  • FIG. 6 shows an alternative embodiment to this in an enlarged area shown analogously to FIG. 5;
  • FIG. 7 shows a schematic illustration to explain the light reflection between a plurality of devices arranged next to one another;
  • FIGS. 8 and 9 are schematic representations of a third exemplary embodiment of the invention
  • FIGS. 10 and 11 are schematic representations of a fourth exemplary embodiment of the invention.
  • the reactor vessel 2 is designed as a flat-bed reactor vessel in which two opposing, flat and parallel walls 3 and 4 and end walls 41 arranged between them form the receiving cavity 5 of the Limit reactor vessel 2.
  • Both of the mutually opposite, flat and parallel walls 3 and 4 of the reactor vessel 2 are each designed to be translucent.
  • On both of these walls 3 and 4 is a diaphragm 7 with a sequence of transparent diaphragm areas 8 and opaque diaphragm areas 9 arranged adjacent thereto.
  • all of the other walls 28 of the reactor vessel 2 which delimit the receiving cavity 5 are opaque and light-absorbing.
  • these other walls 28 delimiting the receiving cavity 5 are the end walls 41 and the intermediate walls 32.
  • the receiving cavity 5 of the reactor vessel 2 is filled with the suspension 6. In the suspension 6 are to be cultivated,
  • the suspension naturally also comprises a liquid, preferably water, and a proportion of carbon dioxide.
  • the suspension can also contain the cultivation, that is to say also the growth and cell division promoting, additional substances, as they have already been mentioned above.
  • FIG. 1 shows a front view of the device 1 and in particular of the reactor vessel 2, on the transparent walls 3 and 4 of which a diaphragm 7 is arranged in each case.
  • 2 shows the section along the section line AA from FIG. 1 through the reactor vessel 2 with the diaphragms 7 arranged on the transparent walls 3 and 4. In FIG. 3, the diaphragms 7 are removed so that the reactor vessel 2 can be seen directly.
  • the diaphragms 7 are fixedly attached to the reactor vessel 2.
  • the relative movement between the suspension 6 and the diaphragms 7 is implemented in this exemplary embodiment by a drive 10 for generating a flow movement of the suspension through the receiving cavity 5.
  • this drive 10 is here as a pump 12 educated.
  • the pump 12 conveys the suspension through the ring line 40, which is only shown schematically here, and the reactor vessel 2 in a circuit that is closed during the cultivation.
  • the suspension with an initial amount of photosynthesis operating microorganisms can be fed into the system via the feed 38 at the beginning of the process.
  • the reactor vessel 2 and the ring line 40 are preferably completely filled with the suspension.
  • the suspension which is then appropriately enriched with microorganisms, can be removed from the system via the withdrawal 39 so that new suspension can then be filled in again via the feed 38.
  • the diaphragms 7 have a sequence of translucent diaphragm areas 8 and opaque diaphragm areas 9.
  • the translucent aperture areas 8 are designed as slits in the aperture 7 and the opaque aperture areas 9 arranged adjacent to each other are designed as webs of the aperture 7.
  • the slots in the diaphragms 7 and / or the webs 7 are preferably designed to be elongated and / or parallel to one another. This can be implemented in this way, but also in other exemplary embodiments of the invention.
  • the drive 10 is a drive for generating a relative movement between the diaphragm 7 and the suspension 6 received in the receiving cavity 5 in a direction 17 transverse, preferably orthogonal, to the longitudinal extension 18 of the slots in the diaphragm 7.
  • This also applies in general, that is to say in this, but also in other preferred embodiments of the invention. Specifically, this is implemented here in such a way that the suspension 6 in the reactor vessel 2 is transported by the pump 12 in a direction 17 which is orthogonal to the The longitudinal extension of the translucent diaphragm areas 8 embodied as slots runs.
  • the diaphragm 7 is arranged on both sides of the reactor vessel 2 in front of the respective light-permeable wall 3 or 4.
  • the diaphragms 7 are fastened to the reactor vessel 2 in a detachable or removable manner from the translucent walls 3 and 4 by means of a releasable fastening device 15. They can be removed manually and / or by machine from the reactor vessel 2 and can be reattached to it.
  • the detachable fastening devices 15 are only roughly indicated in FIG. 2. It can be, for example, clamping mechanisms, clips, screw connections and the like that are known per se.
  • Corresponding fastening devices 15 which are used to fasten and detach two bodies from one another several times, are sufficiently well known in the prior art.
  • the reactor vessel 2 for example, can be cleaned.
  • the diaphragms 7 can be removed in order to expose the microorganisms present in the suspension arranged in the receiving cavity 5 with light over their entire surface in order to bring about a stress situation for the microorganisms. As a result, they can be stimulated to produce certain high-quality substances, which can then ultimately be extracted from the microorganisms and used in pharmaceutical products and / or cosmetics and / or food supplements.
  • the receiving cavity 5 by means of the opaque and / or light-absorbing partition walls 32 are subdivided into meandering flow channels 31 for the suspension 6.
  • the meandering flow channels 31 have the same flow cross section everywhere. In this way, shear forces which interfere with the cultivation of the microorganisms can be avoided.
  • the end walls 41 are also formed correspondingly rounded in this exemplary embodiment.
  • the reactor vessel 2 has at least one inlet 29 for adding suspension 6 to the receiving cavity 5 and at least one outlet 30 for removing suspension from the receiving cavity 5 having.
  • the inlet 29 and the outlet 30 are connected to the ring line 40.
  • the receiving cavity 5, possibly apart from the outlet 30 and the inlet 29, is a self-contained inner volume of the reactor vessel 2. This is advantageous for avoiding unwanted contamination of the suspension during the cultivation of the microorganisms. Even if this is not shown here in the schematic representations according to FIGS.
  • the ring lines 40 as well as the inlet 29 and the outlet 30 advantageously have the same line cross section as the flow channels 31 in the reactor vessel 2. This also helps to avoid unwanted shear forces in the suspension 6 when it flows through the reactor vessel 2 and the ring line 40.
  • FIG. 4 shows a small section of the diaphragm 7 in a view as in FIG. 1, but enlarged.
  • the translucent diaphragm areas 8 embodied as slits and those as webs formed opaque diaphragm areas 9 are preferably each linearly elongated and formed parallel to one another.
  • the longitudinal extension 18 of the translucent diaphragm areas 8 is at least 50% of the width of the respective flow channel 31 of the reactor vessel 2.
  • the longitudinal extension 18 corresponds to at least 90%, preferably 100% of the mentioned width of the flow channel 31, the width of the flow channel 31 in the direction orthogonal to the direction 17 in which the suspension flows through the flow channel 31 is measured.
  • the width 23 of the opaque diaphragm areas 9 is therefore advantageously chosen to be greater than the width 19 of the light-permeable diaphragm areas 8. In this way, certain light scattering effects within the receiving cavity 5 and thus within the suspension 6 can be compensated. this will explained below with reference to FIG. 5.
  • FIG. 5 now shows enlarged a section 7 along the section line BB from FIG. 4 through the reactor vessel 2 with its receiving cavity 5 and the opposed, transparent walls 3 and 4 and the diaphragms 7 attached to them.
  • the respective screen 7 advantageously rests directly on the respective light-permeable wall 3 or 4 of the reactor vessel 2. In this way, light scattering effects between the diaphragm 7 and the translucent wall 3 or 4 are avoided.
  • the light striking the diaphragms 7 from the outside can be both artificial and natural light. Direct irradiation but also scattered light are possible.
  • the light enters through the transparent aperture areas 8 into the receiving cavity 5 and thus into the suspension 6 and thus supplies the microorganisms present in the suspension 6 in the illuminated areas 44 with light or photons.
  • the illuminated areas 44 arranged between the shaded areas 43 within the receiving cavity 5 and the suspension 6 are not hatched.
  • each of the partial volumes 11 of the suspension 6 in the receiving cavity 5 symbolically shown in FIG. 5 is cyclically alternating in an illumination period through one of the translucent aperture areas 8 exposed to light.
  • the microorganisms to be cultivated in the suspension 6 are forcibly exposed to a recurring Hel1 dark cycle, so that the lighting and shading or dark phases required by the Calvin cycle are alternately implemented, so that optimal conditions for carrying out photosynthesis can be set .
  • the flow speed of the suspension in the receiving cavity 5 or in its flow channels 31 is favorably set so that the duration of the lighting periods and / or the shading periods is in a value range from 4 milliseconds to 20 milliseconds, preferably from 4 milliseconds to 10 milliseconds.
  • the suspension 6 is for this purpose by means of the drive 10, here the pump 12 with a Speed in a range of values from 0.25 m / sec (meters per second) to 0.6 m / sec, preferably from 0.3 m / sec to 0.5 m / sec, moved relative to one another.
  • the relative movement between the diaphragm 7 and suspension 6 takes place exclusively through the flow movement of the suspension 6 in the direction 17.
  • both the diaphragm 7 and the suspension 6 and also only the diaphragm 7 can be moved in order to to ensure this relative movement.
  • the transparent diaphragm areas 8 of the diaphragm 7 are each designed as a light-focussing light guide channel 14 having a depth extension 13 in the diaphragm 7.
  • the depth 13 of the respective light-focussing light guide channel 14 measured from a diaphragm surface 16 of the diaphragm 7 facing away from the receiving cavity 5 to a diaphragm surface 24 of the diaphragm facing the receiving cavity 5 is advantageously in a range of values from 6mm to 15mm, preferably from 9mm to 11mm.
  • the light guide channel 14 To prevent light scattering by reflecting light on the, the light guide channel 14, respectively
  • These channel walls 27 can be made of matt black, for example.
  • the panel surface 16 of the panel 7 facing away from the receiving cavity 5 is embodied in a reflective manner.
  • the portion of the light incident on the respective diaphragm 7 which does not penetrate through the transparent diaphragm areas 8 into the interior of the receiving cavity 5 is reflected and is thus available for adjacent devices 1 according to the invention and in particular reactor vessels 2.
  • an orthogonal to the light-permeable wall 3 or 4 measured thickness of the receiving cavity 5 per translucent wall 3 or 4 is in a range of values from 22mm to 26mm, preferably from 23mm to 25mm.
  • the receiving cavity 5 is delimited on both opposite sides by transparent walls 3 and 4.
  • the distance 42 between the two opposed, flat and parallel translucent walls 3 and 4 is in a range of values from 44mm to 52mm, preferably from 46mm to 50mm. This ensures that sufficient photons are available for photosynthesis of the microorganisms for the entire suspension 6 flowing through the receiving cavity 5 or the flow channels 31.
  • FIG. 6 shows in a representation analogous to FIG first embodiment modified embodiment of the invention. It is provided here that only one of the opposing walls 3, which are flat and parallel to one another, is designed as a translucent wall 3 and a screen 7 with a sequence of translucent screen areas 8 and opaque screen areas 9 arranged adjacent to them is arranged on this wall 3 and the other of the opposing walls 33, which are arranged flat and parallel to one another, is impermeable to light. In order to avoid scattering effects, this is opaque trained wall 33 advantageously also light-absorbing, so for example made of matt black.
  • this second exemplary embodiment of the invention illustrated with reference to FIG. 6 has essentially the same features as the first exemplary embodiment, so that reference is made to the description thereof in order to avoid repetition.
  • the sequence according to the invention of translucent aperture areas 8 and opaque aperture areas 9 with a corresponding incidence of light through the translucent aperture areas 8 in the receiving cavity 5 or flow channel 31 generates a sequence of illuminated areas 44 and shaded areas 43, so that the areas flowing in the direction 17 Suspension microorganisms present in each case cyclically alternating in a lighting period through one of the translucent diaphragm areas 8 and shaded in an immediately following shading period by means of one of the opaque diaphragm areas 9.
  • FIG. 7 shows, by way of example and schematically, an arrangement of a plurality of reactor vessels 2 of devices 1 according to the invention of the first exemplary embodiment set up next to one another.
  • the incident light 45 and the light 46 reflected on the mirrored diaphragm surfaces 16 are shown symbolically in the form of arrows.
  • Fig. 7 thus illustrates the effect of the mirrored formations of the diaphragm surfaces 16 of the respective diaphragm 7 facing away from the receiving cavity 5.
  • These mirrored diaphragm surfaces reflect the portion of the light which hits the respective diaphragm 7 but does not penetrate through the transparent diaphragm areas 8 into the receiving cavity 5. This reflected light component is then available for illuminating the adjacent reactor vessels 2. This makes it possible to set up a large number of reactor vessels 2 next to one another in the manner shown schematically in FIG. 7, for example. The mirror surfaces 16 then help to ensure that enough light is available everywhere.
  • FIGS. 8 to 11 now show two examples
  • the receiving cavity 5 is designed as an inherently ring-shaped, in particular circular ring-shaped, closed volume, which is surrounded by an inherently ring-shaped, in particular circular ring-shaped, outer wall 34 of the reactor vessel 2 and by an inherently ring-shaped, in particular circular ring-shaped, Inner wall 35 of the reactor vessel 2 as well as from a bottom wall 36 of the reactor vessel 2 and a top wall 37 of the reactor vessel 2 is limited, at least one of the walls selected from the group consisting of the outer wall 34, the inner wall 35, the bottom wall 36 and the top wall 37, is formed as the translucent wall 3, 4 and the screen 7 on this translucent wall 3, 4 is arranged.
  • the drive 10 is a diaphragm drive for rotating the diaphragm 7 relative to the translucent wall 3, 4, preferably around the translucent wall 3, 4 of the reactor vessel 2.
  • the suspension with the microorganisms can thus largely rest in the receiving cavity 5, while the respective existing diaphragm 7 is moved relative thereto by the respective drive 10, here rotated about a central longitudinal axis 48 in these exemplary embodiments.
  • the drives 10 are only shown very schematically. Correspondingly suitable rotary drives known per se in the prior art can be used for moving the respective diaphragm 7.
  • the diaphragms 7 according to the invention each have a sequence of translucent diaphragm areas 8 and, in addition, in each case adjacently arranged opaque diaphragm areas 9.
  • the transparent diaphragm areas 8 are advantageously designed as elongated, preferably linear, slots and the opaque diaphragm areas 9, preferably as elongated, preferably linear, webs arranged in between.
  • the transparent diaphragm areas 8 are each aligned with their longitudinal extension parallel to the respective central longitudinal axis 48. They expediently extend over at least 50% of the height, preferably over the entire height, of the receiving cavity 5.
  • the diaphragms 7 of the exemplary embodiments according to FIGS. 8 to 11 can, if applicable, analogously to the diaphragms 7 of the first
  • Embodiments be formed.
  • the widths 19 of the translucent diaphragm areas 8, the widths 23 of the opaque diaphragm areas 9, the design of the translucent diaphragm areas 8 as light-focusing light guide channels 14 having a depth extension 13, the depth extension 13 of these light guide channels 14 and the light-absorbing design of the channel walls 27 of these light guide channels 14 Reference can be made to what has been said above about the first exemplary embodiments.
  • the respective diaphragm 7 rests as directly as possible on the translucent wall 3 or 4 of the respective reactor vessel 2.
  • the screen 7 is detachably attached or attachable to the reactor vessel 2 by means of a detachable attachment device 15 from the respective translucent wall 3 or 4 and can be manually and / or mechanically removed from the reactor vessel and attached again.
  • the respective sequence of translucent diaphragm areas 8 and adjacent opaque diaphragm areas 9 ensure the formation of a corresponding sequence of illuminated areas 44 and shaded areas 43 within the respective receiving cavity 5 and thus within the respective suspension 6.
  • the partial volumes 11 of the suspension 6 in the receiving cavity 5 each cycle alternately in an illumination period light is applied to one of the transparent diaphragm areas 8 and is shaded in an immediately following shading period by means of one of the opaque diaphragm areas 9 in each case.
  • the duration of the lighting periods and / or the shading periods are achieved in the preferred value ranges already mentioned at the beginning.
  • the reactor vessel 2 together with the diaphragm 7 is arranged in a self-contained housing 47.
  • the illumination is implemented by a multiplicity of light sources 50 arranged outside the diaphragm 7. These are distributed as evenly as possible around the diaphragm 7 in the sense of an optimal incidence of light through the translucent diaphragm areas 8.
  • the Inner surfaces 49 of the housing 47 are designed to be reflective, so that the light is incident on the diaphragm 7 not only within the light cones 51 of the light source 50, but also as light reflected on the inner surfaces 49 of the housing 47.
  • the reactor vessel 2 is surrounded by the diaphragm 7.
  • the outer wall 34 of the reactor vessel 2 is designed as a translucent wall 3 so that light can penetrate through the translucent aperture areas 8 of the aperture 7 and the translucent wall 3 into the suspension 6 in the receiving cavity 5.
  • the inner wall 35, bottom wall 36 and top wall 37 otherwise delimiting the receiving cavity 5 are formed in this exemplary embodiment as opaque and light-absorbing other walls 28, so that the shaded areas 43 and illuminated areas 44 generated by means of the diaphragm 7 do not reach or through reflections or light input these walls are disturbed.
  • the diaphragm 7, the outer wall 34 and the inner wall 35 are each designed as a circular cylinder. But this does not have to be the case.
  • the receiving cavity 5 is filled with suspension via the feed 38. After the microorganisms have been appropriately cultivated, the suspension correspondingly enriched with microorganisms can then be removed from the receiving cavity 5 via the removal 39.
  • the distance 42 between the light-permeable outer wall 34 and the light-impermeable and light-absorbing inner wall 35 is again advantageously in a range from 22 mm to 26 mm, preferably from 23 mm to

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Abstract

L'invention concerne un dispositif (1) pour la culture de microorganismes photosynthétiques. Le dispositif comprend au moins une cuve de réacteur (2), et la cuve de réacteur (2) comporte au moins une paroi translucide (3, 4) et une cavité de réception (5) pour la réception d'une suspension (6) contenant les microorganismes à cultiver, la paroi translucide (3, 4) délimitant au moins certaines zones de la cavité de réception (5), et le dispositif (1) comportant au moins un écran (7) doté d'une succession de zones d'écran translucides (8) et de zones d'écran (9) non translucides disposées de manière adjacente. L'écran (7) est disposé sur la paroi translucide (3, 4), et le dispositif (1) comporte au moins un dispositif d'entraînement (10) pour générer un mouvement relatif entre l'écran (7) et la suspension (6) reçue dans la cavité de réception (5).
PCT/EP2019/086932 2019-12-23 2019-12-23 Dispositif pour la culture de microorganismes photosynthétiques WO2021129923A1 (fr)

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PCT/EP2019/086932 WO2021129923A1 (fr) 2019-12-23 2019-12-23 Dispositif pour la culture de microorganismes photosynthétiques

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JP2000228973A (ja) * 1999-02-10 2000-08-22 Research Institute Of Innovative Technology For The Earth 藻類培養装置
US20120288921A1 (en) * 2009-12-10 2012-11-15 Guangzhou Institute Of Energy Conversion, Chinese Academy Of Sciences Solar powered spectral photosynthetic bioreactor system for culturing microalgae at high density
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