EP3060683A4 - Procédés et appareil permettant l'amplification et la détection d'acides nucléiques au point d'intervention - Google Patents

Procédés et appareil permettant l'amplification et la détection d'acides nucléiques au point d'intervention Download PDF

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Publication number
EP3060683A4
EP3060683A4 EP14856081.6A EP14856081A EP3060683A4 EP 3060683 A4 EP3060683 A4 EP 3060683A4 EP 14856081 A EP14856081 A EP 14856081A EP 3060683 A4 EP3060683 A4 EP 3060683A4
Authority
EP
European Patent Office
Prior art keywords
detection
methods
point
nucleic acid
acid amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14856081.6A
Other languages
German (de)
English (en)
Other versions
EP3060683A1 (fr
Inventor
Jane P. Bearinger
Scott Castanon
Kenneth J. Michlitsch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Corporos Inc
Original Assignee
Corporos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/262,683 external-priority patent/US9469871B2/en
Application filed by Corporos Inc filed Critical Corporos Inc
Publication of EP3060683A1 publication Critical patent/EP3060683A1/fr
Publication of EP3060683A4 publication Critical patent/EP3060683A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0605—Valves, specific forms thereof check valves
    • B01L2400/0616—Ball valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/065—Valves, specific forms thereof with moving parts sliding valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
EP14856081.6A 2013-10-22 2014-10-22 Procédés et appareil permettant l'amplification et la détection d'acides nucléiques au point d'intervention Withdrawn EP3060683A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361894392P 2013-10-22 2013-10-22
US14/262,683 US9469871B2 (en) 2011-04-14 2014-04-25 Methods and apparatus for point-of-care nucleic acid amplification and detection
PCT/US2014/061808 WO2015061480A1 (fr) 2013-10-22 2014-10-22 Procédés et appareil permettant l'amplification et la détection d'acides nucléiques au point d'intervention

Publications (2)

Publication Number Publication Date
EP3060683A1 EP3060683A1 (fr) 2016-08-31
EP3060683A4 true EP3060683A4 (fr) 2017-08-09

Family

ID=52993515

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14856081.6A Withdrawn EP3060683A4 (fr) 2013-10-22 2014-10-22 Procédés et appareil permettant l'amplification et la détection d'acides nucléiques au point d'intervention

Country Status (5)

Country Link
EP (1) EP3060683A4 (fr)
JP (1) JP2016539633A (fr)
CN (1) CN105874084A (fr)
CA (1) CA2928406A1 (fr)
WO (1) WO2015061480A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106010961B (zh) * 2016-08-03 2019-11-22 珠海百瑞生物科技有限公司 高通量环介导等温扩增鉴定食源性病原体装置及检测方法
EP3342485B1 (fr) * 2017-01-02 2020-07-08 Thinxxs Microtechnology Ag Supports d'éléments de réactifs
US11654429B2 (en) 2017-03-22 2023-05-23 The Board Of Trustees Of The University Of Illinois System for rapid, portable, and multiplexed detection and identification of pathogen specific nucleic acid sequences
EP3606668A1 (fr) * 2017-04-04 2020-02-12 Selfdiagnostics Deutschland GmbH Distribution de composants de lampe dans une cellule à microfluide
US11547997B2 (en) 2017-04-07 2023-01-10 Arizona Board Of Regents On Behalf Of Arizona State University Integrated diagnostic devices having embedded biomolecular computing systems and uses thereof
CN107129930B (zh) * 2017-06-09 2019-11-26 北京百康芯生物科技有限公司 一种全集成核酸检测微流控芯片及其使用方法
DE102017125881B4 (de) 2017-11-06 2019-06-19 Sartorius Stedim Biotech Gmbh Filtermodul und Verfahren zum Nachweis von Mirkoorganismen
CN108642148B (zh) * 2018-07-09 2024-01-30 南京岚煜生物科技有限公司 一种核酸扩增检测微流控芯片及其检测方法
CN112852928A (zh) * 2021-01-14 2021-05-28 广东东阳光药业有限公司 一种多重核酸扩增产物检测方法及试剂盒
US20220235394A1 (en) * 2021-01-25 2022-07-28 Sandra Janeth Perdomo Lara Reverse transcription polymerase chain reaction diagnostic testing device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006018044A1 (fr) * 2004-08-18 2006-02-23 Agilent Technologies, Inc. Ensemble micro-fluidique comprenant des dispositifs micro-fluidiques couples
WO2007011867A2 (fr) * 2005-07-15 2007-01-25 Applera Corporation Dispositif et procede de traitement de fluide
WO2011097412A1 (fr) * 2010-02-03 2011-08-11 President And Fellows Of Harvard College Dispositifs et procédés pour analyses multiplexées

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006122310A2 (fr) * 2005-05-11 2006-11-16 The Trustess Of The University Of Pennsylvania Systeme d'essai
WO2007106579A2 (fr) * 2006-03-15 2007-09-20 Micronics, Inc. Dosages d'acides nucléiques intégrés
WO2008147382A1 (fr) * 2006-09-27 2008-12-04 Micronics, Inc. Dispositifs d'analyse microfluidique intégrés et procédés
AU2011216253B2 (en) * 2010-02-12 2015-07-02 Northwestern University Assay card for sample acquisition, treatment and reaction
US8911941B2 (en) * 2011-04-14 2014-12-16 Kenneth J. Michlitsch Methods and apparatus for point-of-care nucleic acid amplification and detection

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006018044A1 (fr) * 2004-08-18 2006-02-23 Agilent Technologies, Inc. Ensemble micro-fluidique comprenant des dispositifs micro-fluidiques couples
WO2007011867A2 (fr) * 2005-07-15 2007-01-25 Applera Corporation Dispositif et procede de traitement de fluide
WO2011097412A1 (fr) * 2010-02-03 2011-08-11 President And Fellows Of Harvard College Dispositifs et procédés pour analyses multiplexées

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2015061480A1 *

Also Published As

Publication number Publication date
CN105874084A (zh) 2016-08-17
WO2015061480A1 (fr) 2015-04-30
CA2928406A1 (fr) 2015-04-30
EP3060683A1 (fr) 2016-08-31
JP2016539633A (ja) 2016-12-22

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