EP3060683A4 - Verfahren und vorrichtung für nukleinsäureamplifikation und -nachweis am versorgungsort - Google Patents
Verfahren und vorrichtung für nukleinsäureamplifikation und -nachweis am versorgungsort Download PDFInfo
- Publication number
- EP3060683A4 EP3060683A4 EP14856081.6A EP14856081A EP3060683A4 EP 3060683 A4 EP3060683 A4 EP 3060683A4 EP 14856081 A EP14856081 A EP 14856081A EP 3060683 A4 EP3060683 A4 EP 3060683A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- detection
- methods
- point
- nucleic acid
- acid amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0874—Three dimensional network
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0605—Valves, specific forms thereof check valves
- B01L2400/0616—Ball valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/065—Valves, specific forms thereof with moving parts sliding valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361894392P | 2013-10-22 | 2013-10-22 | |
US14/262,683 US9469871B2 (en) | 2011-04-14 | 2014-04-25 | Methods and apparatus for point-of-care nucleic acid amplification and detection |
PCT/US2014/061808 WO2015061480A1 (en) | 2013-10-22 | 2014-10-22 | Methods and apparatus for point-of-care nucleic acid amplification and detection |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3060683A1 EP3060683A1 (de) | 2016-08-31 |
EP3060683A4 true EP3060683A4 (de) | 2017-08-09 |
Family
ID=52993515
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP14856081.6A Withdrawn EP3060683A4 (de) | 2013-10-22 | 2014-10-22 | Verfahren und vorrichtung für nukleinsäureamplifikation und -nachweis am versorgungsort |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP3060683A4 (de) |
JP (1) | JP2016539633A (de) |
CN (1) | CN105874084A (de) |
CA (1) | CA2928406A1 (de) |
WO (1) | WO2015061480A1 (de) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106010961B (zh) * | 2016-08-03 | 2019-11-22 | 珠海百瑞生物科技有限公司 | 高通量环介导等温扩增鉴定食源性病原体装置及检测方法 |
EP3342485B1 (de) * | 2017-01-02 | 2020-07-08 | Thinxxs Microtechnology Ag | Halterung für reagenzträgerelemente |
CA3057326A1 (en) | 2017-03-22 | 2018-09-27 | The Board Of Trustees Of The University Of Illinois | System for rapid, portable, and multiplexed detection and identification of pathogen specific nucleic acid sequences |
WO2018185143A1 (en) * | 2017-04-04 | 2018-10-11 | Selfdiagnostics Deutschland Gmbh | Lamp component distribution in a microfluid cell |
WO2018187687A1 (en) * | 2017-04-07 | 2018-10-11 | Arizona Board Regents On Behalf Of Arizona State University | Integrated diagnostic devices having embedded biomolecular computing systems and uses thereof |
CN107129930B (zh) * | 2017-06-09 | 2019-11-26 | 北京百康芯生物科技有限公司 | 一种全集成核酸检测微流控芯片及其使用方法 |
DE102017125881B4 (de) | 2017-11-06 | 2019-06-19 | Sartorius Stedim Biotech Gmbh | Filtermodul und Verfahren zum Nachweis von Mirkoorganismen |
CN108642148B (zh) * | 2018-07-09 | 2024-01-30 | 南京岚煜生物科技有限公司 | 一种核酸扩增检测微流控芯片及其检测方法 |
CN112852928A (zh) * | 2021-01-14 | 2021-05-28 | 广东东阳光药业有限公司 | 一种多重核酸扩增产物检测方法及试剂盒 |
US20220235394A1 (en) * | 2021-01-25 | 2022-07-28 | Sandra Janeth Perdomo Lara | Reverse transcription polymerase chain reaction diagnostic testing device |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006018044A1 (en) * | 2004-08-18 | 2006-02-23 | Agilent Technologies, Inc. | Microfluidic assembly with coupled microfluidic devices |
WO2007011867A2 (en) * | 2005-07-15 | 2007-01-25 | Applera Corporation | Fluid processing device and method |
WO2011097412A1 (en) * | 2010-02-03 | 2011-08-11 | President And Fellows Of Harvard College | Devices and methods for multiplexed assays |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006122312A2 (en) * | 2005-05-11 | 2006-11-16 | The Trustees Of The University Of Pennsylvania | Methods of testing using a microfluidic cassette |
AU2007225038B2 (en) * | 2006-03-15 | 2013-08-29 | Perkinelmer Health Sciences, Inc. | Integrated nucleic acid assays |
WO2008147382A1 (en) * | 2006-09-27 | 2008-12-04 | Micronics, Inc. | Integrated microfluidic assay devices and methods |
CN102939543B (zh) * | 2010-02-12 | 2015-06-24 | 西北大学 | 用于样本采集、处理和反应的测定卡 |
US8911941B2 (en) * | 2011-04-14 | 2014-12-16 | Kenneth J. Michlitsch | Methods and apparatus for point-of-care nucleic acid amplification and detection |
-
2014
- 2014-10-22 EP EP14856081.6A patent/EP3060683A4/de not_active Withdrawn
- 2014-10-22 CA CA2928406A patent/CA2928406A1/en not_active Abandoned
- 2014-10-22 CN CN201480070428.1A patent/CN105874084A/zh active Pending
- 2014-10-22 WO PCT/US2014/061808 patent/WO2015061480A1/en active Application Filing
- 2014-10-22 JP JP2016526159A patent/JP2016539633A/ja active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006018044A1 (en) * | 2004-08-18 | 2006-02-23 | Agilent Technologies, Inc. | Microfluidic assembly with coupled microfluidic devices |
WO2007011867A2 (en) * | 2005-07-15 | 2007-01-25 | Applera Corporation | Fluid processing device and method |
WO2011097412A1 (en) * | 2010-02-03 | 2011-08-11 | President And Fellows Of Harvard College | Devices and methods for multiplexed assays |
Non-Patent Citations (1)
Title |
---|
See also references of WO2015061480A1 * |
Also Published As
Publication number | Publication date |
---|---|
CA2928406A1 (en) | 2015-04-30 |
CN105874084A (zh) | 2016-08-17 |
JP2016539633A (ja) | 2016-12-22 |
EP3060683A1 (de) | 2016-08-31 |
WO2015061480A1 (en) | 2015-04-30 |
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Legal Events
Date | Code | Title | Description |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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17P | Request for examination filed |
Effective date: 20160517 |
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AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
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AX | Request for extension of the european patent |
Extension state: BA ME |
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DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20170710 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12M 1/36 20060101ALI20170704BHEP Ipc: C12Q 1/68 20060101AFI20170704BHEP |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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18D | Application deemed to be withdrawn |
Effective date: 20180207 |