EP2818245B1 - Seringue, famille de seringues et dispositif de dosage - Google Patents

Seringue, famille de seringues et dispositif de dosage Download PDF

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Publication number
EP2818245B1
EP2818245B1 EP14174635.4A EP14174635A EP2818245B1 EP 2818245 B1 EP2818245 B1 EP 2818245B1 EP 14174635 A EP14174635 A EP 14174635A EP 2818245 B1 EP2818245 B1 EP 2818245B1
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EP
European Patent Office
Prior art keywords
syringe
centering
metering device
region
plunger
Prior art date
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Application number
EP14174635.4A
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German (de)
English (en)
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EP2818245A1 (fr
Inventor
Michael Blumentritt
Jürgen Löhn
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Eppendorf SE
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Eppendorf SE
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41629839&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2818245(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP14174635.4A priority Critical patent/EP2818245B1/fr
Priority to EP19154709.0A priority patent/EP3498376B1/fr
Priority to DK19154709.0T priority patent/DK3498376T3/da
Priority to DK14174635.4T priority patent/DK2818245T3/da
Publication of EP2818245A1 publication Critical patent/EP2818245A1/fr
Application granted granted Critical
Publication of EP2818245B1 publication Critical patent/EP2818245B1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means

Definitions

  • the invention relates to a metering device with a syringe or with a syringe family.
  • Pipettes are dosing devices for measuring and transferring liquids. They are often designed as repeating or multipette, which are used together with a syringe to receive liquid into the syringe and gradually release from it.
  • a repeating pipette is from the DE 29 26 691 C2 and the US 4,406,170 known, which in particular describes the repeating mechanism of Repetierpipette. It also describes the fixation of the syringe on the repeating pipette.
  • the syringe has a syringe flange, which can be inserted from the side into a laterally open, substantially U-shaped groove of the pipette.
  • An axial pressure spring fixes the syringe flange used in the groove.
  • an insert element is provided, which receives an end portion of the syringe plunger between two jaws.
  • the jaws can be pressed against the syringe plunger by means of a flap-shaped clamping member whose actuating lever projects out of the housing through an opening.
  • the Ep 1 524 035 A1 describes another pipette in which the syringe is inserted from the side into a receptacle.
  • the DE 43 41 229 C2 and the US 5,620,660 propose a more suitable for the manual operation pipette system with a simple axially inserted into the pipette or removable from this syringe.
  • This pipette system has a syringe having a mounting portion and a syringe plunger, and a syringe Pipette having a receptacle for the attachment portion and a receiving body with a piston receptacle for the syringe piston or a piston rod connected thereto in a pipette housing.
  • fastening devices for the reversible or releasable fixing of the fastening section and syringe piston in the receptacles and piston adjusting devices for displacing the receiving body in the pipette housing are provided.
  • the attachment portion and the syringe plunger are axially slidable into their attachment positions by axial openings of their receptacles.
  • the fastening devices have radially deliverable gripping devices for fixing the fastening section and the syringe piston in the fastening positions.
  • the gripping means have pivotally mounted in the pipette syringe gripping lever and pivotally mounted in the receiving body piston gripping lever.
  • the syringe gripping levers and the plunger gripping levers are designed as two-armed with a gripping arm and an actuating arm, the syringe gripping levers having contact points on the insides of their actuating arms which, by actuating their actuating arms, are pivotable outwardly against the actuating arms of the plunger gripping levers and actuate the plunger gripping levers.
  • the attachment portion is a syringe flange and according to another embodiment, the syringe piston has a piston collar for engaging behind by the gripping devices.
  • EP 1 724 020 A1 and the US 2006263261 A1 is an embodiment of the pipette according to DE 43 41 229 C2 in which the syringe can be detached from the pipette by actuating only a single trigger.
  • the EP 2 033 712 A1 describes a further development of this pipette, in which the Syringe discharge by means of an actuator of the piston adjusting devices takes place so that no separate trigger for the syringe discharge is required.
  • the commercial versions Multipette® (plus / stream / Xstream) of this dosing system from Eppendorf AG include Combitips® (plus) syringes, which have a cylinder with a piston running area on the inside and a syringe flange on the top of the cylinder.
  • the syringes are offered with different filling volumes (0.1 ml, 0.2 ml, 0.5 ml, 1 ml, 2.5 ml, 5 ml and 10 ml).
  • the internal diameter of the piston running area is dimensioned so that the filling volume is taken into the syringe at a maximum piston stroke of the Multipette® of Eppendorf AG of 50 mm plus an overstroke of a few millimeters and / or released from it. Accordingly, for the syringe having a nominal volume of 10 ml, the inner diameter of the piston barrel is 15.96 mm. In the smaller syringes, the inner diameter of the piston running range is correspondingly smaller.
  • the cylinders of the smaller syringes have a cylindrical extension at the upper end of the piston running range, the inner diameter of which is in each case significantly smaller than 15.96 mm and which is the smaller, the smaller the filling volume of the syringe.
  • cylindrical extension allows a depth stop of the piston in the cylinder can be realized, thereby preventing that the piston sealing lip is damaged by placing the sealing lip on the lower end face of the cylinder.
  • the piston area is made thicker below the inserted into the dosing coupling piece, in particular designed as a wing piece, thereby achieving an increase in the buckling stiffness of the piston rod in storage condition.
  • the cylinders starting from the flange on the circumference, have four outwardly projecting, axially extending ribs distributed evenly around the circumference.
  • the ribs each have a heel below for support at the edge of holes a tray or a box.
  • the heels of syringes of different fill volumes In order for syringes of different fill volumes to be inserted into unitary holes of a carrier or box, the heels of syringes of different fill volumes have the same distances from the axis of the cylinder.
  • the known dosing system allows accurate dosages with low errors.
  • the object of the invention is to provide a metering device with a syringe or with a syringe family, which enables even more accurate dosages.
  • a metering device with a syringe or with a syringe family with the features of claim 1.
  • top and bottom refer to the preferred orientation of the syringe during dosing, in which the syringe is held vertically with the outlet at the bottom and the centering collar at the top.
  • the metering device can be a manually and / or motor-driven, manageable and / or stationary metering device.
  • it can be a pipette, repeating pipette, dispenser, dosing station or automatic dosing machine.
  • it is a dispenser.
  • the syringe according to the invention can be reversibly fixed in a reversible manner to a metering device according to the invention.
  • the syringe is inserted into the metering device in the axial direction, the centering collar being gripped by means of radially deliverable gripping means for gripping the centering collar in the receptacle of the metering device and the syringe plunger being gripped on its coupling piece in the piston receptacle by radially deliverable gripping devices for gripping the piston rod.
  • the gripping of the centering collar and the coupling piece by the gripping means can be controlled by the axial insertion of the syringe into the receptacle of the metering device.
  • the metering device can be configured as in the DE 43 41 229 C2 and the US 5,620,660 described.
  • the centering element penetrates axially into the centering area and engages radially against the inside of the centering area.
  • the dimensions and geometries of the centering and the centering are coordinated so that the syringe barrel is centered by the inserted centering.
  • the syringe barrel is guided with the centering exactly in the recording, so that it can be easily and safely gripped by the gripping devices.
  • the centering element can be a non-circular shape of the syringe barrel correct an ideal circular shape. Since the centering area is either in the immediate vicinity of the piston running range, which is crucial for the exact dosage, or in the immediate vicinity of the transition region, which counteracts damage to the sealing lip of the piston, the correction in the centering area has a positive effect on the dosing accuracy.
  • a non-circular cylinder has a cross-sectional area changed to the circular cylinder, which leads to metering errors.
  • the guidance of the centering on the centering prevents misalignment of the syringe to the metering device and tilting of the syringe with respect to the metering device under load.
  • Misalignment can occur with a conventional metering system when the syringe is gripped in tilted orientation by the gripping means of the metering system.
  • a tipping under load can occur in a conventional dosing system, when dosing the syringe is placed in the vicinity of its exit with a pressing force on a wall. This delivery form is called wall delivery. In particular, it is used at small delivery volumes to avoid errors due to uneven drops from the syringe.
  • the centering element rests at a distance from the upper end of the syringe barrel at the centering. Only when the centering engages correspondingly deep in the syringe plunger, it can invest all around to the centering and correct a non-circular shape of the syringe barrel, align it and support and prevent tilting.
  • the stiffening of the syringe barrel by the centering collar at the upper end of the syringe is opposed to the correction of a non-circular cross section in the piston running area.
  • the second inner diameter of at least 16.2 mm of the centering area of the syringe according to the invention or the centering area of the syringes of the syringe family according to the invention is at least at a distance of at least 3 mm from the upper end of the syringe barrel, i. in close proximity to the piston running area or to the transition area.
  • the centering area is therefore also characterized by its immediate proximity to the piston running area.
  • the syringe plunger can be inserted with its coupling piece through the axial passage of the centering element, so that the syringe plunger can be pulled out of the syringe barrel.
  • the piston receptacle engages for coupling the coupling piece in the axial passage of the centering element.
  • the inner diameter in the centering area of at least 16.2 mm ensures that syringes with a filling volume of up to 10 ml with an inner diameter of the piston running range of up to 15.96 mm in a metering device with a maximum piston travel of 50 mm plus an overstroke of a few millimeters can be used.
  • syringes covered all common filling volumes. Larger filling volumes (eg 25-50 ml) are possible if an adapter is used which reduces the larger diameter of the syringe barrel to the smaller diameter of the dosing device holder.
  • An adapter is also useful for small-volume syringes, as it facilitates the grip and handling of small syringes.
  • the adapter has the centering area.
  • the adapter is also centered in the metering device, thus directing the syringe, supporting it and preventing the syringe from tilting, especially in the case of wall dispensing.
  • the invention relates to syringes in which the syringe barrel is in one piece. Furthermore, it includes syringes in which the syringe barrel is in several parts. In particular, it involves syringes in which the syringe barrel has the piston barrel portion and the exit at a lower portion and the centering collar and centering portion at an upper portion connectable to the lower portion, e.g. by bayonet, screw or snap connection.
  • the upper part may in particular be an adapter.
  • the inner diameter of the centering of the syringe according to the invention exceeds the inner diameter of the piston running range, so that the syringe barrel during injection molding filled more evenly with plastic and thus can be made dimensionally stable.
  • the jump in the inner diameter between piston running area and centering area causes namely a flow brake, the below the centering injected hot plastic mass forces, preferably first fill the lower part of the syringe barrel and thus the piston barrel area uniformly and finally the centering and the centering collar.
  • the dimensional accuracy of the syringe barrel is improved straight in the piston barrel area and thus the precision of the dosage.
  • a jump in the inner diameter between the piston running area and centering area allows the syringe plunger, which is completely inserted into the syringe barrel, to be guided at the top in the piston running area if the syringe is not connected to the metering device.
  • the outer diameter of a guide portion of the syringe plunger arranged below the coupling piece can almost correspond to the inner diameter of the piston barrel portion.
  • the outside diameter of the guide area should be less than the inside diameter of the piston running area by at least the sum of the tolerances of syringe plungers (eg 0.1 mm) and syringe barrels (eg 0.1 mm).
  • the centering of the syringe plunger through the guide area in the separate syringe from the syringe avoids tilting of the syringe plunger in the syringe barrel and thus deformations in the sealing region of the syringe plunger and / or the syringe barrel by partial irreversible expansion, thereby dosing errors are avoided.
  • the leadership of the syringe plunger at the top of the piston running range can by outwardly projecting axially extending wings of the syringe plunger and / or by a Disc can be effected below the coupling piece of the syringe plunger.
  • the disc can also cause a coverage of the piston running area, which prevents ingress of contaminants in the syringe barrel, which affect the sealing of the piston and / or can contaminate the liquid to be dispensed.
  • the jump in the inner diameter between the centering area and the piston running area allows a simple tolerance check by means of force measurement during the joining of syringe plungers and syringe barrels during production. If the diameter of the syringe plunger is too large, two force increases are measured during joining, namely when the syringe plunger with its sealing area penetrates into the centering area and then when the sealing area penetrates into the piston barrel area. If the diameter of the piston does not exceed the tolerance upper limit, an increase in force is only measured when the sealing area penetrates the piston running area. By simple and unambiguous measurement can thus be determined whether the diameter of the syringe plunger exceeds the tolerance upper limit and the syringe plunger must be discarded.
  • Checking compliance with the upper tolerance limit is particularly important because roundness of the syringe plunger can be determined as an increase in the diameter.
  • the use of syringe plungers, which cause dosing errors due to a non-circular sealing area, can thus be avoided. Since the tolerances of the syringe plunger and the syringe barrel are included in the test, when the tolerance upper limit is exceeded, preferably the entire syringe is discarded.
  • the present invention thus also includes a method for tolerance testing.
  • the first When inserting a syringe plunger into the syringe barrel, the force for inserting the syringe plunger into the syringe barrel is measured and, in the event that the force for inserting the syringe plunger into the centering area exceeds a tolerance upper limit, the syringe plunger and / or the syringe barrel are discarded ,
  • the syringe can be easily released from the dosing system after use by operating the gripping means.
  • the separation can also take place in an axial movement. By centering a tilting of the syringe barrel is avoided in the recording, so that the separation can be made easier.
  • the syringe can fall out of the dosing system after releasing the gripping devices due to their own weight. This can optionally be supported by a sprung stop on which the centering collar of the syringe barrel can be fixed.
  • the inner diameter in the centering region of the syringe according to the invention is at most 17.7 mm.
  • the limitation of the inner diameter in the centering area to a maximum value of 17.7 mm ensures that the syringes can be gripped by means of a conventional Eppendorf AG Multipette® dispenser if they are held in a carrier.
  • the gripping devices for releasably fixing the syringe flange have hook-shaped gripping ends, the inner edges of which, when pivoted together, have a distance of 20.8 mm from each other.
  • the syringes may be provided in a carrier having support sleeves protruding from a plate.
  • the syringes can be inserted into holes of the carrier sleeves, wherein the syringe cylinder is guided on the outer circumference of the centering in the carrier sleeves. So that the syringe gripper levers do not clamp on the carrier sleeves, the outer diameter of the carrier sleeves can be selected so that it is the distance between the swiveled Gripper ends fall below. Taking into account a minimum wall thickness of the carrier sleeves, a clearance for inserting the syringe barrels into the carrier sleeves and a minimum wall thickness for the syringe barrel in the centering area, the inner diameter in the centering area is at most 17.7 mm.
  • the syringe is preferably made of plastic, in particular a thermoplastic.
  • the thermoplastic is selected from the group of polyolefins.
  • Preferred polyolefins are polyethylene (PE), polypropylene (PP) and cyclic olefin (co) polymers, COC for short or COP.
  • Syringe and piston can be made of the same plastic or different. In particular, due to the better friction pairing, the piston is made of PE and the cylinder is made of PP or vice versa.
  • the syringe according to the invention can be designed so that it can also be used in conventional dispensers of the Multipette® type from Eppendorf AG, which do not have a centering element.
  • the advantageous effects of the syringes according to the invention are basically also given in the syringes of the syringe family according to the invention.
  • the centering regions have contours which are matched to the same predetermined centering contour, so that they can be used with the same metering device whose centering element has the centering contour.
  • the user can use a syringe with a suitable filling volume in each application and always the dosing accuracy is improved by the centering of the respective syringe in the metering device.
  • the syringes with different filling volumes of the syringe family preferably have the same contours in the centering regions. You can, too at least partially have different contours, provided that they are tuned to one and the same Zentrierkontur.
  • cylindrical and polygonal contours can be matched to the same cylindrical centering, so that the provided with these different contours centering areas are centered by the same centering.
  • the centering regions of the syringes with different filling volumes of a syringe family thus have a functionally matching contour, which is matched to the same predetermined centering contour.
  • a matching contour in the centering area thus means the same and / or different contours or geometries, which interact with the same centering element of a metering device, which has the predetermined centering contour, ie are complementary thereto.
  • the matching contour or geometry in the centering selected from the group of conical hollow body, the convex hollow body, the pyramidal hollow body, the columnar hollow body with ellipsoidal outer ground or cross-sectional area, the polygonal columnar, in particular5.3teckklalenförmigen hollow body, the hollow body with circumferential or partially encircling ribs or webs, the hollow body with at least two flattened inner sides, or the hollow body, which are cut by at least two secants, the hollow body with projections, warts, webs and the elliptical hollow body, and a combination thereof.
  • Preferred combinations are conical with circumferential bead, eg as torus, a combination of conical and cylindrical and conical with bars or round elevations.
  • the predetermined centering contour of the centering element is also selected from the aforementioned groups.
  • the syringes of the syringe family in the centering region have an inner diameter of at least 16.2 mm and at most 17.7 mm.
  • the syringe family can have a syringe with a filling volume of 10 ml, which has the same inner diameter of 15.96 mm in the piston running region and in a centering region.
  • a second inner diameter of at least 16.2 mm is an advantageous embodiment of the syringes of the syringe family according to the invention, which ensures that even a syringe with a filling volume of 10 ml has the advantages of a centering region with a larger inner diameter than in the piston barrel region.
  • the syringe barrel has a greater wall thickness in the cylindrical piston running area than in the centering area.
  • the syringe barrel has a conical insertion area at the upper end of the centering area and / or a conical area between the centering area and the cylindrical piston running area Transition area.
  • the conical lead-in area and / or the conical transition area guide the syringe plunger during insertion into the piston barrel area, so that the sealing area of the plunger is correctly inserted and not damaged.
  • the insertion region and / or the transition region have an angle of 15 to 30 ° to the axis of the syringe barrel. Particularly preferred is an angle of the insertion of about 15 ° and an angle of the transition region of about 25 °.
  • the centering area can be designed differently. It can be designed inside as a freeform surface or a ruled surface.
  • a free-form surface is understood to mean a surface that can not be written exactly in mathematical terms, whereas a ruled surface can be described exactly mathematically.
  • it has inside the contour or geometry of a surface of revolution (eg cylinder, cone, hyperbola, parabolic surface).
  • the centering is slightly conical with a cone angle of 0.5 to 5 °, in particular about 2 ° to the longitudinal axis of the syringe.
  • the contour or geometry of the centering can be smooth inside.
  • the imaginary largest inscribed circle corresponds to the outer diameter of the centering element.
  • the contour or geometry of the centering leads, in cooperation with a complementary centering contour or geometry of the centering element to a correction of an optionally non-circular shape of the syringe barrel, to align and support the syringe plunger and thus prevents tilting of the piston in the Syringe barrel.
  • the interaction of the contour or geometry of the centering area with the complementary centering contour or geometry of the centering element enables the correct alignment of a syringe coding on a sensor for scanning the syringe coding and / or an anti-twist device.
  • the anti-rotation device prevents twisting of the syringe in the dosing device, which can damage a sensor for sensing syringe coding.
  • the contour may also serve to reduce friction between syringe barrel and centering member to facilitate insertion of a smooth centering member into the centering area.
  • the contour or geometry can affect the imaginary / projected largest inscribed circle only at two diametrically opposite points, whereby already a centering is achieved. Preferably, it affects the circle at more than two places. Preferably, the spots are evenly distributed over the circumference of the circle.
  • the centering element of a metering system which cooperates with the syringes of the syringe family, has an outer diameter of 16.2 to 17.7 mm and / or a wall thickness of 0.4 to 2.5 mm and / or stands with respect to a stop for the top of Centering collar 2.2 to 6 mm in front.
  • the centering collar has an outer diameter of 21 to 24.2 mm and / or a height of 3.2 to 5.4 mm and / or the centering area is at least at a distance of a minimum of 3 mm and a maximum of 6 mm from the upper end the syringe barrel. This allows insertion of the syringe into the commercial dispensers of the Multipette® type from Eppendorf AG.
  • the syringe has a filling volume selected from the volumes 10 ml, 5 ml, 2.5 ml, 1 ml, 0.5 ml, 0.2 ml, 0.1 ml. Always ensures the Centering an exact centering on a centering of a dosing.
  • the syringe piston below the coupling piece has a disc and / or axially extending wings and is present between the disc and / or the wings and the centering an annular gap with a gap width of 0.5 to 2.5 mm.
  • the disc prevents contaminants from entering the syringe barrel and the disc and / or wings guide the syringe barrel at the top of the barrel.
  • Through the annular gap with a gap width of 0.5 to 2.5 mm ensures that the centering between disc and / or wing and syringe barrel fits and the disc and / or wings are inserted into the centering.
  • the centering element may in particular be a torus or hollow cylinder of low height. Further, it may be formed as a hollow cylinder greater height, hollow cone or as another continuous rotating body, so that a line contact or surface contact with the centering is possible. Even a multi-point contact can be useful.
  • the centering element may be pyramidal or polygonal, with the pyramid or polygonal column having any number of corners (e.g., 8).
  • the centering element is cylindrical, in particular circular cylindrical, or slightly conical, in particular with a cone angle of 0.1 to 2.5 °, in particular about 1 °.
  • the centering element preferably has a conical insertion region at the lower end.
  • the centering element on the outside of a contour with projections and / or depressions, wherein the smallest circle circumscribing the contour has the second inner diameter.
  • This contour can mesh with a complementary contour of the syringe.
  • This can be a Syringe coding can be aligned directly on a sensor of the metering device. Furthermore, this can be effected by a rotation, which prevents the syringe from twisting in the coding device. Furthermore, the contour can reduce the friction when inserting the centering element in the syringe by interacting with a smooth centering.
  • the centering element is arranged rigidly with respect to the receptacle or is sprung with respect to the receptacle in the axial direction.
  • the rigid arrangement causes a stiffer guidance of the syringe with respect to the metering device compared to the sprung arrangement.
  • the rigid arrangement has the advantage that a sprung stop on which the centering collar rests with its upper side, supports the release of the syringe from the metering device.
  • the advantage of the rigid arrangement is that it can prevent tilting when the syringe is applied with a certain force to a substrate, e.g. a vessel edge, is placed, for example, as usual in the already discussed pipetting technique of wall delivery.
  • a spring-loaded centering element can compensate for alignment errors when inserting the centering tube into the receptacle.
  • the centering element is spring-loaded with respect to the receptacle by being fixed to a spring-loaded stop for the centering collar.
  • the centering element in the contact region with the centering region has an outer diameter of 16.2 to 17.7 mm and / or a wall thickness of 0.4 to 2.5 mm and / or stands with respect to a stop for the upper side of the centering collar by 2 , 2 to 6 mm in front.
  • This information relates to the state of the metering device in which the syringe is not used, and include both relatively spring-loaded and relative to each other rigid arrangements of centering and stop. If the stopper is designed as a sensor plate, they relate to the base of the sensor plate from which possibly contact elements protrude.
  • Fig. 1 has a 10 ml syringe 1.1 a syringe barrel 2.1, in which a syringe plunger 3.1 is arranged.
  • the syringe barrel 2.1 is not strictly cylindrical everywhere in geometric terms.
  • the syringe barrel 2.1 has an externally cylindrical cylinder section 4.1, which has a cylindrical piston running area 5.1 inside.
  • the cylinder section 4.1 has a bottom bottom 6.1 with a central hole 7.1.
  • the edge of the hole 7.1 is connected to an inside and outside conical tip section 8.1 with a low cone angle, which has an inside and outside more conical conical section 9.1 below.
  • the syringe section 8.1 and the cone section 9.1 taper downwards.
  • the cylinder section 4.1 is connected at the top via a short inside and outside conical transition region 10.1 with an inside and outside cylindrical or slightly conical centering 11.1.
  • the centering region 11.1 is connected at the top to a centering collar 12.1, which has a substantially circular disk-shaped contact section 13.1 and a substantially cylindrical jacket section 14.1.
  • the contact section 13.1 has a contact surface 15.1.
  • This may have codings in the form of axially directed sensing surfaces arranged at one or more defined positions. The codings may be formed as in the EP 0 657 216 B1 and the US 5,620,661 described.
  • bottom 6.1, tip section 8.1 and transition region of the syringe barrel 2.1 has an approximately constant wall thickness.
  • the syringe barrel 2.1 is made in one piece from plastic. It is preferably produced by injection molding of a thermoplastic.
  • the thermoplastic is, for example, polypropylene; Polyethylene or a cyclic olefinic (CO) polymer, polypropylene being preferred for its stability properties.
  • the syringe piston 3.1 has a flat cylindrical or circular piston-shaped piston portion 17.1, which has a circumferential sealing lip 18.1 on the circumference. Below, the piston portion 17.1 is connected to a piston tip portion 19.1, which is conically formed with the same or a similar small cone angle as the tip section 8.1.
  • the syringe piston 3.1 is provided at the top with a piston rod 20.1.
  • the piston rod 20.1 has a coupling piece 21.1 on top.
  • the coupling piece 21.1 has a plurality of radially projecting, circumferential piston collars 22.1, between which circumferential grooves 23.1 are present.
  • a disk 24.1 is arranged on the piston rod 20.1, which is formed circular disk-shaped.
  • the wings 25.1 are connected to the disc 24.1 and at the bottom to the piston portion 17.1.
  • the wings 25.1 have adjacent to the disc 24.1 outer edges 26.1 which are parallel to the piston rod 20.1.
  • the wings 25.1 have another bevel 29.1, within which the width of the wings 25.1 continues to decrease.
  • the syringe piston 3.1 is preferably made in one piece from plastic. Further preferably, it is injection molded from a thermoplastic. For example, it is made of polyethylene or polypropylene, with polyethylene being preferred, because the material pairing of polyethylene in the piston and polypropylene in the cylinder has a low frictional force. Polypropylene is preferred for the cylinder because of its high strength.
  • the cylindrical sections and areas of the syringe barrel 2.1 and the syringe plunger 3.1 are circular cylindrical.
  • the outer edges 26.1 extend from the upper end of the piston running area 5.1 via the transition area 10.1 into the centering area 11.1.
  • the disk 24.1 engages below in the centering 11.1.
  • Between the outer edges 26.1 and the piston running area 5.1 remains a small annular gap, so that the outer edges 26.1 prevent the syringe plunger 3.1 from tilting in the syringe barrel.
  • Between the disc 24.1 and the outer edges 26.1 and the centering 11.1 a slightly larger annular gap 290.1 is present.
  • the coupling piece 21.1 is partially arranged in the centering area 11.1 and projects beyond the centering collar 12.1 upwards.
  • a 0.1 ml syringe 1.2 has a syringe barrel 2.2 and a syringe plunger 3.2.
  • the syringe barrel 2.2 is not strictly cylindrical everywhere in geometric terms. It has at the bottom an inside and outside cylindrical cylinder section 4.2, which has a cylindrical piston running region 5.2 inside.
  • the lower end of the cylinder section 4.2 is connected to an inside and outside conical tip section 8.2, which is formed as a more conical cone section 9.2.
  • the upper end of the cylinder section 4.2 is connected to an inner and outer conical transition region 10.2.
  • the upper end of the transition region 10.2 is connected to an inner and outer cylindrical centering 11.2.
  • the centering area 11.2 is in turn connected at the upper end with a centering collar 12.2.
  • the centering collar 12.2 has a substantially circular disk-shaped contact section 13.2, which is connected at the inner edge with the centering 11.2. Further, the centering collar 12.2 has a substantially cylindrical shell portion 14.2, which is connected at the upper end to the outer edge of the abutment portion 13.2.
  • the contact section 13.2 has a contact surface 15.2 on top, which may have 15.1 codes according to the contact surface.
  • the syringe piston 3.2 has a long cylindrical piston portion 17.2, the bottom of the circumference has a circumferential sealing lip 18.2.
  • the piston portion 17.2 is connected at the bottom with a piston tip portion 19.2 having a cylindrical portion at the top and a conical portion at the bottom.
  • the piston portion 17.2 is provided at the top with a piston rod 20.2, which has a coupling piece 21.2 above, which is formed according to the coupling piece 21.1.
  • a disk 24.2 is arranged on the piston rod 20.2. Below the disc 24.2 project from the piston rod 20.2 radially four wings 25.2, which are distributed uniformly over the circumference of the piston rod 20.2.
  • the wings 22.1 are connected at the top to the underside of the disk 24.2 and end at the bottom just above the piston section 17.2. They point above each to the piston rod portion 20.2 parallel outer edges 26.2 and below each have a chamfer 27.2.
  • the chamfers 27.2 sit inside on the transitional area 10.2. Between the piston tip section 19.2 and the cone section 9.2 of the syringe barrel 2.2 remains a small annular gap.
  • the disk 24.2 is arranged below in the centering 11.2. Between centering 11.2 and disc 24.2 a small annular gap 290.2 is present.
  • the coupling piece 21.2 is partially arranged in the centering area 11.2 and projects above the centering collar 12.2.
  • Fig. 1a and 2a have the syringes 1.1, 1.2 on the circumference of their Zentrierbunde 12.1, 12.2 radially outwardly projecting Ausrichtnasen 30.1, 30.2, which taper upwards.
  • the alignment noses 30.1 and 30.2 have a parabolic contour at the top. In the example, in each case seven alignment noses 30.1, 30.2 are distributed uniformly over the circumference of the centering collars 12.1, 12.2.
  • Fig. 1e and 2e has the centering 11.1, 11.2 above an inner diameter of 16.2 to 17.7 mm and extends to a depth d of 6mm. Furthermore, the piston running range 5.1, 5.2 has an inner diameter b of 15.96 or 1.60 mm.
  • the centering collar 12.1, 12.2 has a height e of 3.2 to 5.4 mm and an outer diameter b of 21 to 24.2 mm ..
  • the coupling piece 22.1, 22.2 is opposite the Zentrierbund 12.1, 12.2 about 13.5mm before.
  • the coupling piece 21.1, 21.2 has a diameter g of 7 to 7.2 mm and in the region of the grooves 23.1, 23.2 a diameter h of preferably less than 5 mm.
  • This area is preferably designed as a crossed web construction for mass reduction.
  • Fig. 1g are concrete dimensions of the centering 11.1 of the 10 ml syringe 1.1 indicated. Accordingly, the centering 11.1 has a minimum diameter of 16.2 mm and a maximum diameter of 17.7 mm.
  • the centering region 11.1 begins at a distance of 3 mm from the top of the centering collar 12.1 and extends over an axial length of 3 mm.
  • Fig. 1g is the centering area 11.1 shown free of hatching, although the syringe barrel 2.1 is cut vertically in the centering 11.1.
  • Fig. 1h also shows the syringe barrel 2.1 in a vertical section, in which the centering area 11.1 is shown free of hatching, as this can basically assume different geometries.
  • Preferred embodiments of this freeform surface or regular shaping surface are shown in partial sections to the right of the vertical section through the entire syringe barrel 2.1. In total, six geometries are indicated, of which three are shown in a vertical section and another three in a half section through the centering 11.1.
  • the uppermost vertical partial section shows a conically configured centering 11.11.
  • the second vertical partial section shows an indicated convex centering area 11.12.
  • the third vertical partial section shows a circumferential or partially encircling centering area 11.13 provided with (optionally rounded) ribs or webs. This last type of embodiment can also serve as a demolding aid.
  • the lowermost, vertical partial vertical section serves to illustrate the viewing direction from below through the syringe barrel 2.1 onto the centering region 11.1, which can be configured with different geometries. Different configurations are then shown on the far right in the half cross sections. It are half cross sections shown, since it is about cross sections through the vertical section shown by the syringe cylinder 2.1 shown to the left.
  • the uppermost half cross-section represents a flattened centering area 11.14 cut by a plurality of secants.
  • the central half cross section shows a centering area 11.15 provided with projections or warts or webs.
  • the lowest half cross-section shows an elliptical cut centering 11.16.
  • the smallest diameter which is at a distance of at least 3 and preferably not more than 6 mm below the top of the centering collar 12.1, is a maximum of 16.2 to 17.7 mm.
  • the outer diameter of a - explained below - centering ring for centering the centering must also have these dimensions, otherwise the centering does not fit into the centering and can not fulfill its function. This is indicated on the centering area 11.14 by a radius is drawn there.
  • Fig. 3 are the other syringes 1.3 to 1.7 of the syringe family with the filling volumes 5 ml, 2.5 ml, 1 ml, 0.5 ml and 0.2 ml constructed accordingly.
  • the parts of the other syringes 1.3 to 1.7, which correspond to those of the syringes 1.1, 1.2 described above, are provided with corresponding reference numerals, wherein the number mentioned before the point coincides and the number mentioned after the point denotes the respective syringe.
  • Fig. 3b are the flow brakes below the centering 11.1, 11.2, 11.3 illustrated. Accordingly, between the centering regions 11.1, 11.2, 11.3 and the transitional regions 10.1, 10.2, 10.3, there are corners in which the plastic compound is deflected during injection molding, so that they form a flow brake. In addition, the centering regions 11.1, 11.2, 11.3 have a smaller wall thickness than the adjacent transitional regions 10.1, 10.2, 10.3. In the case of the 10 ml syringe, the centering area 11.1 has a larger inner diameter than the piston running area 5.1. As a result, pressure losses are caused during injection molding, which have a uniform filling of the cylinder sections 4.1, 4.2, 4.3 with hot plastic mass result.
  • an injection molding tool 31 includes a tool plate 32 having a hot runner nozzle 33, a tool cover plate 34, and a stripper plate 35. Further, it has a tool core 36. Between the aforementioned components, a cavity 37 is formed, which images the contour of a 0.1 ml syringe barrel 2.2.
  • the hot plastic mass is injected through the hot runner nozzle 33 into the cavity 37. Due to the flow brake between the transition region 10.2 and the centering 11.2 of the transition region 10.2 and the cylinder section 4.2 of the syringe barrel 2.2 are evenly filled, so that the syringe barrel 2.2 there has a very good dimensional stability. This is also contributed to that is injected into the transition region 10.2, in which the tool core 36 has a relatively large diameter, so that no adverse lateral deflection of the tool core 36 occurs, which leads to uneven wall thicknesses.
  • the injection-molded syringe barrel 2.2 is ejected from the injection mold 31 by the stripper plate 35 of the tool plate 32nd subtracted and the tool core 36 is pulled out of the stripper plate 35. The injection molding 2.2 then falls out of the tool 31.
  • FIGS. 5 and 6 show how the jump of the inner diameter between the centering 11.1 and piston 5.1 range of the 10 ml syringe 1.1 can be used for the determination of not dimensionally accurate syringe plunger 3.1, in which the outer diameter of the piston portion 17.1 in the region of the sealing lip 18.1 exceeds the tolerance upper limit.
  • the inner diameter of the centering region 11.1 is designed so that it corresponds to the tolerance upper limit of the outer diameter of the sealing lip 18.1.
  • the inner diameter of the piston running range 5.1 is 15.96 mm.
  • corresponding parts of various syringe plungers 3.1 whose dimensions differ due to manufacturing tolerances are distinguished by reference numerals having a different numeral in the second position behind the dot.
  • the diameter of the in Fig. 5 in the left position shown sealing lip 18.11 is too large. According to Fig. 6 are determined when inserting this piston portion 17.11 of a built-in insertion mechanism force gauge (load cell) two KraftanTDstressn. This clearly indicates that a too large syringe plunger 3.1 is present.
  • the lack of dimensional stability can be based in particular on an ovality of the piston portion 17.11, which has an inflated outer diameter of the sealing lip 18.11 result, which can be determined by the test method described above.
  • the in Fig. 5 shown in the right position piston portion 17.12 is with the sealing lip 18.12 within the tolerance or too small. According to Fig. 6 In this syringe plunger 3.1, only a single force increase stage is measured.
  • FIGS. 7 and 8 show a further embodiment with which it is also easy to determine whether the piston portion 17.1 falls below the tolerance lower limit.
  • This embodiment has a centering region consisting of three centering 11.11, 11.12, 11.13, wherein the inner diameter of the centering 11.12 smaller than the inner diameter of the centering 11.11 and the inner diameter of the centering 11.13 smaller than the inner diameter of the centering 11.12.
  • the centering section 11.13 is adjoined by a piston running region 5.1, the inner diameter of the piston running region 5.1 in turn being smaller than the inner diameter of the centering section 11.13.
  • the inner diameters are designed so that the inner diameter of the centering 11.11 corresponds to the tolerance upper limit of the outer diameter of the sealing lip 18.1 of the piston portion 17.1.
  • the inner diameter of the centering 11.12 corresponds to the lower engagement limit of the outer diameter of the sealing lip 18.1 and the inner diameter of the Zentrierbabiteses 11.13 of the lower tolerance limit for the sealing lip 18.1.
  • Fig. 7 second piston portion 17.12 shown from the left has a sealing lip 18.12 with an outer diameter at the upper tolerance limit.
  • this piston section 17.12 When inserting this piston section 17.12 are according to Fig. 8 three power increase levels measurable. This syringe piston 3.1 is within the tolerance range.
  • Fig. 7 in the third position shown from the left piston section 17.13 has at the sealing lip 18.13 an outer diameter at the lower tolerance limit.
  • Fig. 8 are two force increase stages visible when inserting this piston section 17.13. This syringe piston 3.1 is still in the tolerance range.
  • the centering sections 11.11 to 11.13 can also be referred to as test sections. This is the case in particular if only a part of the centering sections 11.11 to 11.13 is used for centering. In principle, however, all centering 11.11 to 11.13 can be used for centering. The centering will be explained below.
  • Fig. 9 has a metering device according to the invention 370 designed as an elongate handle housing 38.
  • a substantially circular cylindrical receptacle 39 for the centering collar 12 of the syringe barrel 2 is present.
  • the receptacle 39 is accessible through a circular opening 40 at its lower end from the outside.
  • a plurality of axially directed guide tabs 41 are radially inwardly a plurality of axially directed guide tabs 41 before.
  • a receiving body 42 with a substantially cylindrical piston receptacle 43 for a coupling piece 21 of a syringe plunger 3 is present in the housing 38.
  • the piston receptacle 43 is accessible from the outside by a further circular opening 44 at its lower end.
  • the receiving body 42 is axially displaceable in the housing 38 by means of a repeating mechanism, not shown.
  • the repeating mechanism may in particular be designed as in the DE 29 26 691 C2 and the US 4,406,170 or the DE 43 41 229 C2 and the US 5,620,660 described.
  • the repeating mechanism comprises an elevator lever 440, which is axially displaceable on the outside of the housing 38, wherein in Fig. 9 the elevator lever 440 is shown in the lowermost position. Accordingly, the receiving body 42 is in the lowermost position, in which its lower end is disposed approximately at the level of the bottom of the receptacle 39.
  • the repeating mechanism comprises a metering lever 45, which protrudes from the top of the housing 38 and is pivotally mounted in the housing 38.
  • the metering lever 45 is loaded by a spring device so that it is pressed into its uppermost position.
  • By pivoting the metering lever 45 down the receiving body 42 by a metering step is displaced downwards.
  • the step size of the metering step is adjustable by means of a Einstellrädchens 46, which is located at the upper end of the housing 38.
  • two diametrically opposed syringe gripping levers 47 are arranged below in the housing 38.
  • the syringe gripping levers 47 are pivotally mounted in the housing 38.
  • the syringe gripping levers 47 have outwardly projecting actuating keys 48 from the housing 38. They pass through apertures in the housing 38 and have hook-like cylinder gripping ends 49 protruding into the receptacle 39.
  • two plunger gripping levers 50 are mounted diametrically opposite one another in the receptacle body 42. The piston gripping levers 50 penetrate through openings in the receiving body 42 so that they engage with hook-like piston gripping ends 51 in the piston receptacle 43.
  • the syringe gripping levers 47 and piston gripping levers 50 are each designed with two arms with a gripping arm and an actuating arm and are pivotably mounted in the connecting region of the gripping arm and the actuating arm. Furthermore, the syringe gripping levers 47 on the inner sides of their actuating arms unlock cam and the piston gripping lever 50 on the actuating arms cam-like projections. Not shown spring elements load the syringe gripping lever 47 and the piston gripping lever 50 so that they are each pivoted toward each other with their Zylinderieriden 49 and piston gripping ends 51.
  • the construction corresponds to the embodiment according to DE 43 41 229 C2 and US 5,620,660 ,
  • a scanning device 52.1 for encoding on a centering collar 12 of a syringe 1 is present, which in Fig. 10 and 11 is shown.
  • the scanning device 52.1 has an annular disk-shaped sensor plate 53 with a central hole 54 and seven axially projecting nubs 55, which are distributed uniformly over the underside of the sensor plate 53.
  • Each nub 55 is operatively connected to a microswitch disposed in the sensor plate 53.
  • the microswitch operatively connected thereto is actuated.
  • the scanning device 52.1 comprises a carrier plate 56 for the sensor plate 53.
  • the sensor plate 53 can be fixed to the carrier plate 56 by means of bolts 57, which pass through the holes 58 of the sensor plate 53.
  • the support plate 56 has a central hole 59 whose diameter corresponds to the diameter of the hole 54 of the sensor plate 53.
  • a nikhohlzylindharis centering element 60.1 (also called “centering tube”) fixed with axial passage, the outside at the bottom has a chamfer 61.
  • the centering element 60.1 engages through the hole 54 of the sensor plate 53 when the sensor plate 53 is fixed to the carrier plate 56 by means of the bolts 57.
  • pins 62 From the top of the support plate 56 are protruding pins 62, wherein in each case a pin 62 is arranged at one corner.
  • the pins 62 are fixedly connected to the carrier plate 56.
  • a holding plate 64 is present, which also has a central hole 65 whose diameter corresponds approximately to the diameter of the holes 54, 59.
  • the holding plate 64 has at the corners in each case a bore 66, in each of which a pin 62 of the support plate 56 can be inserted.
  • support plate 64 axially directed spring hooks 67 on each opposite sides, each with a hook end 68 which slide on assembly of support plate 56 and retaining plate 64 by lateral grooves 69 of the support plate 56 and finally with the hook ends 68 engage over the underside of the support plate 56.
  • the spring hooks 67 hold the assembly of support plate 56 and retaining plate 64 together.
  • a sensor plate 53 Details of a sensor plate 53 are in the embodiment of EP 0 657 216 B1 and the US 5,620,661 described.
  • a syringe 1 is attached to the scanner 52.1.
  • the syringe 1 rests against the sensor plate 53 with the upper side of the centering collar 12.
  • arranged on the contact surface coding can be scanned by means of the nubs 55.
  • centering element 60.1 with axial passage engages in the centering collar 12. In this case, centering element 60.1 rests with an axial passage on the cylindrical centering region 11.
  • sensor plate 53 The above-described arrangement of sensor plate 53, support plate 56, centering element 60.1 with axial passage and retaining plate 64 is in the housing 38 of the metering device 370 of Fig. 9 built-in.
  • the holding plate 64 is fixed in the housing 38.
  • the sensor plate 53 forms the bottom of the receptacle 39. From this floor, the centering element 60.1 is in the receptacle 39 into it.
  • the receiving body 42 is accessible through the opening 40 of the receptacle and the holes 59 and 65 of the scanner 52.1 from the outside.
  • Fig. 12 shows a metering device 370 with an inserted syringe 1.
  • the syringe 1 is inserted with the centering collar 12 through the opening 40 in the receptacle 39.
  • the syringe 1 is guided by the alignment lugs 30 on the guide lugs 41, so that the codings on the contact surface 15 are assigned to the knobs 55 exactly.
  • the syringe gripping levers 47 slide due to the spring effect with their Zylindersammlungden 49 over the edge of the centering collar 12 until they snap under its underside. If the contact surface 15 at the Sensor plate 53 is applied, the centering collar 12 is engaged behind at the bottom of the Zylindersammlungden 49 of the syringe gripping lever 47.
  • the receiving body 42 When inserting the syringe 1, the receiving body 42 is arranged in its lowest position. In this position, the coupling piece 21 engages in the piston receptacle 43. The piston gripping levers 50 slide with their gripping ends 51 over the coupling piece 21 until they snap behind a piston collar 22 into a groove 23.
  • the centering element with axial passage 60.1 penetrates into the centering area 11, so that the syringe 1 is fixed in the metering device 370.
  • the syringe 1 is accurately positioned in its seat in the receptacle 39 and the piston receptacle 43, so that it is exactly gripped by the gripping means 47, 50. Furthermore, the centering element with axial passage 60.1 corrects any irregularities of the syringe barrel 2. Furthermore, the centering element 60.1 keeps the syringe 1 in the correct orientation, even if the spigot 8 of the syringe 1 is placed and a transverse force is introduced into the syringe 1.
  • Fig. 13 shows an alternative scanning device 52.2, in which the centering element 60.2 is fixed to a support plate 64.
  • the scanning device 52.2 can be mounted in the metering device 370 instead of the scanning device 52.1.
  • the holding plate 64 is fixed in the housing 38.
  • the syringe 1 is subject to an even lower deflection under load by a transverse force.
  • Fig. 14 has a syringe 1 in the centering 11 axially extending grooves 70. Furthermore, the centering element with axial passage 60.3 has circumferentially axially extending ribs 71 which fit into the grooves 70. When the syringe 1 is pushed onto the centering element 60.3, the intermeshing of grooves 70 and ribs 71 prevents the syringe 1 from rotating relative to the receptacle 39. This prevents damage to the sensor plate 53.
  • a lid 72 of a box, not shown, or a tray has a plate 73 which has a plurality of upwardly projecting carrier sleeves 74.
  • Syringes 1.1, 1.2, 1.4 of different sizes are placed with their centering collar 12.1, 12.2, 12.4 on top of the edge of the carrier sleeves 74. They are guided on the outer circumference of the centering region 11.1, 11.2, 11.4 in the carrier sleeves 74.
  • the outer conical transition regions 10.1, 10.2, 10.4 ensure that the syringes 1.1, 1.2, 1.4 are guided exactly in the correct holding position.
  • Fig. 16 has a syringe 1.8 a syringe barrel 2.8 with a cylinder section 4.8, which has a cylindrical piston barrel 5.8 inside.
  • the cylinder section 4.8 has a bottom bottom 6.8 with a central hole 7.8, the edge of which has a conical tip section 8.8 of a more conical cone section 9.8 at the lower end.
  • the cylinder section 4.8 has at the upper edge two diametrically opposed, radially outwardly projecting projections 75 of a bayonet connection.
  • the syringe cylinder 2.8 comprises an adapter 76, which is formed substantially sleeve-shaped.
  • the adapter 76 has on the inside of the lower edge outgoing axial grooves 77 of the bayonet joint, which at an axial Distance from the bottom ends.
  • the axial grooves 77 are arranged on two diametrically opposite inner sides of the adapter 76. Top open the axial grooves 77 in circumferential grooves 78 of the bayonet connection.
  • the syringe barrel 2.8 is in Fig. 16 with the projections 75 inserted through the axial grooves 77 and screwed into the circumferential grooves 78, to the end of the respective circumferential groove 78.
  • This bayonet connection 75, 77, 78 of the syringe barrel 2.8 is connected to the adapter 76.
  • the adapter 76 has a centering area 11.8 above an intermediate floor 79, which delimits the circumferential grooves 78 above.
  • the adapter 76 has at the top of a radially outwardly projecting centering collar 12.8, the top has a contact surface 15.8 with codings.
  • the intermediate bottom 79 has a large passage opening 80 through which a syringe piston 3.8 is inserted into the syringe barrel 2.8.
  • the syringe piston 3.8 has a piston section 17.8 with a circumferential sealing lip 18.8 on the circumference.
  • the piston rod 20.8 has a coupling piece 21.8 on top. Below the coupling piece 21.8, the piston rod 20.8 is provided with a disc 24.8 and sit underneath radially projecting wings 25.8 on the piston rod 3.8.
  • This syringe 1.8 can be used with the adapter 76 in the receptacle 39 and the coupling piece 21.8 in the receiving body 42 of a metering device 370, as for the syringe 1 based on Fig. 12 is explained above.
  • the adapter is guided by the centering element 60.1 in the centering area, so that the syringe 1.8 is centered.
  • the examined syringes are also referred to as “Combitips®”, the dosing devices examined also as “Multipette®” and the centering element as “centering tube” or “centering sleeve” or “centering ring”.
  • the prototypes were compared with a standard Multipette® X-Stream. For the experiments two Multipettes® were rebuilt. The safety mats with the mounting plates were changed as follows.
  • Fig. 17a the centering sleeve is shown not spring-mounted on the support plate.
  • the arrangement of Fig. 17a corresponds to the in Fig. 13 shown arrangement.
  • Fig. 17b the centering sleeve is attached to the bottom, so that the centering sleeve is mitfedert.
  • the arrangement of Fig. 17b corresponds to the in the Figures 10 and 11 shown arrangement.
  • the used serial components of a Multipette® Dispenser have not been changed.
  • the measurements were carried out on a train / printing machine (Z005 type) from Zwick Roell.
  • test speed was 50mm / min.
  • the measurements were started after 0.1N pre-power.
  • Fig. 18 shows the comparison of a conventional dispenser of the type Mulitipette® with a metering device according to the invention with sprung centering sleeve and with a metering device according to the invention with non-spring-loaded centering.
  • a series Multipette® was used.
  • Measurements 5 and 6 were made with a prototype Mulipette® with a spring-loaded centering sleeve - as in Fig. 17b shown.
  • a graphical representation of the results is in Fig. 18b a shows the results with the conventional dispenser, b the results with the dispenser with the spring-loaded centering sleeve and c the results with the dispenser with the non-springed centering sleeve.
  • the examined syringes are also referred to as “Combitips®”, the dosing devices examined also as “Multipette®” and the centering element as “centering tube” or “centering sleeve” or “centering ring”.
  • the examination was performed with a standard Multipette® X-stream and a 50ml Combitip® plus. For metrological reasons, the largest Combitip® was selected for this test.
  • test speed was 50mm / min.
  • the measurements were started after 0.1N pre-power.

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Claims (16)

  1. Dispositif de dosage avec une seringue (1), la seringue (1) présentant les caractéristiques suivantes :
    un cylindre de seringue (2) et
    un piston de seringue (3), le cylindre de seringue (2) présentant une sortie sur le dessous, un collier de centrage sur le dessus sur sa périphérie externe, une zone de course de piston cylindrique (5) reliée à la sortie, dans laquelle le piston de seringue (3) est engagé de façon étanche, ledit piston de seringue (3) présentant une pièce de couplage (21) à son extrémité supérieure,
    ou avec une famille de seringues comptant plusieurs de ces seringues, et le dispositif de dosage présentant les caractéristiques suivantes :
    - un boîtier (38) comportant
    - un logement (39) pour le collier de centrage (12) du cylindre de seringue (2) avec une ouverture axiale (40) dans laquelle est inséré axialement le collier de centrage (12) dans une position de fixation,
    - un corps de logement (42) avec un logement de piston (43), dans lequel est insérée une pièce de couplage (21) dans une position de fixation,
    - des dispositifs de fixation (47, 50) destinés à maintenir de manière amovible le collier de centrage (12) et la pièce de couplage (21) dans leurs positions de fixation dans le logement (39) et dans le logement de piston (43),
    - les dispositifs de fixation (47, 50) présentant des dispositifs de préhension aptes à être approchés radialement pour saisir le collier de centrage (12) et la pièce de couplage (21) dans les positions de fixation, et
    - des dispositifs de réglage de piston pour le déplacement du corps de logement (42) dans le boîtier (38),
    caractérisé en ce que
    - le cylindre de seringue (2) dispose d'une zone de centrage (11) cylindrique du cylindre de seringue (2) plus élevée que la zone de course du piston (5) et
    - en ce que le dispositif de dosage dispose d'un élément de centrage (60) avec passage axial, lequel est disposé dans le logement (39) et dirigé vers l'ouverture axiale (40) et lequel pénètre axialement dans la zone de centrage (11) cylindrique du cylindre de seringue (2) lors de l'insertion de la seringue dans le dispositif de dosage, tout en s'appliquant radialement sur le côté intérieur de la zone de centrage (11).
  2. Dispositif de dosage avec seringue ou famille de seringues selon la revendication 1, le cylindre de seringue (2) et le piston de seringue (3) étant fabriqués à partir d'un seul et même plastique ou de plastiques différents.
  3. Dispositif de dosage avec seringue ou famille de seringue selon la revendication 1 ou 2, la seringue présentant une zone de course cylindrique du piston (5) avec un premier diamètre intérieur et le cylindre de seringue (3) présentant, à une distance minimale de 3 mm de son extrémité supérieure, la zone de centrage (11) disposant d'un deuxième diamètre intérieur supérieur au premier diamètre intérieur et mesurant au moins 16,2 mm et tout au plus 17,7 mm.
  4. Dispositif de dosage avec seringue ou famille de seringues selon les revendications 1 à 3, la pièce de couplage (21) pouvant être introduite à travers le passage axial de l'élément de centrage (60) de sorte que le piston de seringue (3) peut être extrait du cylindre de seringue (3).
  5. Dispositif de dosage avec seringue ou famille de seringues selon les revendications 1 à 4, l'épaisseur de paroi du cylindre de seringue (2) étant plus grosse dans la zone de course cylindrique du piston (5) que dans la zone de centrage (11).
  6. Dispositif de dosage avec seringue ou famille de seringue selon les revendications 1 à 5, la seringue présentant une zone d'introduction (16) à l'extrémité supérieure de la zone de centrage (11) et/ou, entre la zone de centrage (11) et la zone de course cylindrique du piston (5) du cylindre de seringue (2), une zone de transition conique (10) servant à guider le piston de seringue (3) lorsque celui-ci est introduit dans la zone de course du piston (5).
  7. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 6, l'intérieur de la zone de centrage (11) présentant un contour avec des saillies ou des cavités (69), le plus gros cercle imaginaire inscrit dans le contour présentant le deuxième diamètre intérieur.
  8. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 7, dans lequel le collier de centrage (12) présente un diamètre extérieur d'environ 21 à 24 mm et/ou une hauteur d'environ 3,2 à 4,5 mm, et/ou dans lequel la zone de centrage présente un écart d'au moins 3 à 6 mm par rapport à l'extrémité du cylindre de seringue.
  9. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 8, dans lequel le piston de seringue (3) présente, dans sa portion supérieure, en dessous d'une pièce de couplage (21), un disque (24) et/ou des ailes (25) s'étendant axialement, une fente annulaire (290) d'une largeur de 0,5 à 2,5 mm entre le disque (24) et/ou les ailes (25) et la zone de centrage cylindrique.
  10. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 9, la seringue présentant une capacité de remplissage sélectionnée parmi les volumes 10 ml ; 5 ml ; 2,5 ml ; 1 ml ; 0,5 ml ; 0,2 ml ; 0,1 ml.
  11. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 10, l'élément de centrage (60) du dispositif de dosage présentant, dans la zone de contact avec une zone de centrage, un diamètre externe de 16,2 à 17,7 mm et/ou une épaisseur de paroi de 0,4 à 2,5 mm, et/ou faisant saillie de 2,2 à 6 mm par rapport à une butée pour la face supérieure du collier de centrage (12).
  12. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 11, l'extérieur de l'élément de centrage (60) du dispositif de dosage présentant un contour avec des saillies (70) et/ou des cavités, le plus petit cercle décrivant le contour présentant un deuxième diamètre interne.
  13. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 12, l'élément de centrage (60) étant disposé de façon rigide par rapport au logement (39) ou chargé sur ressort dans la direction axiale par rapport au logement (39).
  14. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 13, le diamètre intérieur de la zone de centrage (11) de la seringue (1) dépassant le diamètre intérieur de la zone de course du piston (5).
  15. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 14, les zones de centrage (11) des seringues (1) avec différents volumes de remplissage de la famille de seringues présentant des contours adaptés au même contour de centrage prédéfini de l'élément de centrage (60), de sorte qu'ils peuvent être utilisés avec le même dispositif de dosage dont l'élément de centrage présente le contour de centrage.
  16. Dispositif de dosage avec seringue ou famille de seringues selon l'une des revendications 1 à 15, dans lequel les seringues (1) avec différents volumes de remplissage de la famille de seringues présentent des contours identiques dans la zone de centrage (11).
EP14174635.4A 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage Active EP2818245B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP14174635.4A EP2818245B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
EP19154709.0A EP3498376B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
DK19154709.0T DK3498376T3 (da) 2009-07-27 2009-07-27 Sprøjte, sprøjtefamilie og doseringsindretning
DK14174635.4T DK2818245T3 (da) 2009-07-27 2009-07-27 Sprøjte, sprøjtefamilie og doseringsindretning

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14174635.4A EP2818245B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
EP09009693.4A EP2279791B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP09009693.4A Division-Into EP2279791B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
EP09009693.4A Division EP2279791B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP19154709.0A Division-Into EP3498376B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
EP19154709.0A Division EP3498376B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage

Publications (2)

Publication Number Publication Date
EP2818245A1 EP2818245A1 (fr) 2014-12-31
EP2818245B1 true EP2818245B1 (fr) 2019-07-17

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EP14174635.4A Active EP2818245B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
EP19154709.0A Active EP3498376B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
EP09009693.4A Active EP2279791B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage

Family Applications After (2)

Application Number Title Priority Date Filing Date
EP19154709.0A Active EP3498376B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage
EP09009693.4A Active EP2279791B1 (fr) 2009-07-27 2009-07-27 Seringue, famille de seringues et dispositif de dosage

Country Status (3)

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EP (3) EP2818245B1 (fr)
DK (2) DK3498376T3 (fr)
PL (1) PL2279791T3 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011114591B3 (de) 2011-09-30 2012-12-27 Eppendorf Ag Spritze für den Gebrauch mit einer Dosiervorrichtung, Spritzensystem umfassend mehrere Spritzen für den Gebrauch mit einer Dosiervorrichtung, Dosiervorrichtung für den Gebrauch mit einer Spritze, Dosiersystem umfassend eine Spritze und eine Dosiervorrichtung und Verfahren zum Betreiben eines Dosiersystems
EP3260822B1 (fr) 2016-06-21 2020-07-15 Eppendorf Ag Seringue multivoie pour l'utilisation a l'aide d'un dispositif de dosage
EP3831486A1 (fr) * 2019-12-06 2021-06-09 Eppendorf AG Famille de pointes de pipette comprenant les pointes de pipette destinées à l'utilisation avec des pipettes d'une famille de pipettes et famille de pipettes comprenant les pipettes destinées à l'utilisation avec des pointes de pipette d'une famille de pointes de pipette

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2926691C2 (de) 1979-07-02 1983-05-26 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Repetierpipette
US4099548A (en) 1976-08-25 1978-07-11 Oxford Laboratories Inc. Hand-held pipette for repetitively dispensing precise volumes of liquid
FR2515339A1 (fr) 1981-10-28 1983-04-29 Marteau D Autry Eric Pipette de precision a deplacement positif
DE4341229C2 (de) 1993-12-03 1995-09-07 Eppendorf Geraetebau Netheler Pipettensystem
DE4342178C2 (de) * 1993-12-10 1995-09-28 Eppendorf Geraetebau Netheler Pipettensystem
FI116613B (fi) * 2003-10-17 2006-01-13 Biohit Oyj Pipetin kärjen kiinnitysmenetelmä ja laite menetelmän toteuttamiseksi
DE102004063652B4 (de) * 2004-12-31 2007-02-08 Tecpharma Licensing Ag Vorrichtung zur dosierten Verabreichung eines fluiden Produkts mit koaxialer Dosisanzeige
DE102005023203B4 (de) 2005-05-20 2009-06-04 Eppendorf Ag Pipette
DE102007042115B4 (de) * 2007-09-05 2010-12-02 Eppendorf Ag Pipette
WO2009046482A1 (fr) * 2007-10-10 2009-04-16 Sge Analytical Science Pty Ltd Système pour seringue

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Also Published As

Publication number Publication date
EP2279791B1 (fr) 2014-11-12
DK3498376T3 (da) 2021-12-20
DK2818245T3 (da) 2019-10-21
PL2279791T3 (pl) 2015-04-30
EP2818245A1 (fr) 2014-12-31
EP2279791A1 (fr) 2011-02-02
EP3498376A1 (fr) 2019-06-19
EP3498376B1 (fr) 2021-09-15

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