EP2674438A3 - Overexpressierendes Baculovirentransactivatorensystem zur Produktion von rekombinanten Proteinen - Google Patents

Overexpressierendes Baculovirentransactivatorensystem zur Produktion von rekombinanten Proteinen Download PDF

Info

Publication number
EP2674438A3
EP2674438A3 EP12196120.5A EP12196120A EP2674438A3 EP 2674438 A3 EP2674438 A3 EP 2674438A3 EP 12196120 A EP12196120 A EP 12196120A EP 2674438 A3 EP2674438 A3 EP 2674438A3
Authority
EP
European Patent Office
Prior art keywords
recombinant proteins
baculovirus
sequences
vectors
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12196120.5A
Other languages
English (en)
French (fr)
Other versions
EP2674438A2 (de
Inventor
Silvia Gomez Sebastian
Javier LÓPEZ VIDAL
José Angel MARTINEZ ESCRIBANO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alternative Gene Expression SL ALGENEX
Original Assignee
Alternative Gene Expression SL ALGENEX
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES12750333.2T priority Critical patent/ES2612854T3/es
Priority to ES12753914.6T priority patent/ES2611302T3/es
Priority to JP2015516476A priority patent/JP6143853B2/ja
Priority claimed from PCT/EP2012/061088 external-priority patent/WO2012168493A2/en
Application filed by Alternative Gene Expression SL ALGENEX filed Critical Alternative Gene Expression SL ALGENEX
Priority to EP12196120.5A priority patent/EP2674438A3/de
Priority to CA2893578A priority patent/CA2893578A1/en
Priority to PCT/EP2013/075812 priority patent/WO2014086981A1/en
Priority to US14/649,500 priority patent/US9879280B2/en
Priority to EP13811407.9A priority patent/EP2928290B1/de
Priority to ES13811407.9T priority patent/ES2554752T3/es
Publication of EP2674438A2 publication Critical patent/EP2674438A2/de
Publication of EP2674438A3 publication Critical patent/EP2674438A3/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14131—Uses of virus other than therapeutic or vaccine, e.g. disinfectant
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16051—Methods of production or purification of viral material
EP12196120.5A 2012-06-12 2012-12-07 Overexpressierendes Baculovirentransactivatorensystem zur Produktion von rekombinanten Proteinen Withdrawn EP2674438A3 (de)

Priority Applications (9)

Application Number Priority Date Filing Date Title
ES12750333.2T ES2612854T3 (es) 2012-06-12 2012-06-12 Elementos de ADN recombinante para la expresión de proteínas recombinantes en una célula huésped
ES12753914.6T ES2611302T3 (es) 2012-06-12 2012-06-12 Elementos de ADN recombinante para la expresión de proteínas recombinantes en insectos
JP2015516476A JP6143853B2 (ja) 2012-06-12 2012-06-12 宿主細胞における組換えタンパク質の発現のためのバキュロウイルスｄｎａエレメント
EP12196120.5A EP2674438A3 (de) 2012-06-12 2012-12-07 Overexpressierendes Baculovirentransactivatorensystem zur Produktion von rekombinanten Proteinen
ES13811407.9T ES2554752T3 (es) 2012-12-07 2013-12-06 Sistema de baculovirus para expresar proteínas que forman partículas de tipo virus
CA2893578A CA2893578A1 (en) 2012-12-07 2013-12-06 Baculovirus system for expressing proteins forming virus-like particles
PCT/EP2013/075812 WO2014086981A1 (en) 2012-12-07 2013-12-06 Baculovirus system for expressing proteins forming virus-like particles
US14/649,500 US9879280B2 (en) 2012-12-07 2013-12-06 Baculovirus system for expressing proteins forming virus-like particles
EP13811407.9A EP2928290B1 (de) 2012-12-07 2013-12-06 Baculovirussystem zur expression von proteinen zur bildung virusähnlicher partikel

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
PCT/EP2012/061088 WO2012168493A2 (en) 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in insects
PCT/EP2012/061081 WO2012168492A2 (en) 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in a host cell
PCT/RU2012/000456 WO2012169940A2 (en) 2011-06-10 2012-06-13 Recombinant dna elements for the expression of recombinant proteins in insects
PCT/RU2012/000455 WO2012169939A2 (en) 2011-06-10 2012-06-13 Recombinant dna elements for the expression of recombinant proteins in a host cell
EP12196120.5A EP2674438A3 (de) 2012-06-12 2012-12-07 Overexpressierendes Baculovirentransactivatorensystem zur Produktion von rekombinanten Proteinen

Publications (2)

Publication Number Publication Date
EP2674438A2 EP2674438A2 (de) 2013-12-18
EP2674438A3 true EP2674438A3 (de) 2014-02-19

Family

ID=47290832

Family Applications (2)

Application Number Title Priority Date Filing Date
EP12196120.5A Withdrawn EP2674438A3 (de) 2012-06-12 2012-12-07 Overexpressierendes Baculovirentransactivatorensystem zur Produktion von rekombinanten Proteinen
EP13811407.9A Active EP2928290B1 (de) 2012-12-07 2013-12-06 Baculovirussystem zur expression von proteinen zur bildung virusähnlicher partikel

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP13811407.9A Active EP2928290B1 (de) 2012-12-07 2013-12-06 Baculovirussystem zur expression von proteinen zur bildung virusähnlicher partikel

Country Status (6)

Country Link
US (1) US9879280B2 (de)
EP (2) EP2674438A3 (de)
JP (1) JP6143853B2 (de)
CA (1) CA2893578A1 (de)
ES (3) ES2612854T3 (de)
WO (1) WO2014086981A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104735977B (zh) 2012-06-12 2016-09-21 替代基因表达公司 用于在宿主细胞中表达重组蛋白的杆状病毒dna元件
WO2014086973A1 (en) * 2012-12-07 2014-06-12 Alternative Gene Expression S.L. Enhanced production of the porcine circovirus capsid protein by a baculovirus vector expression system
ES2554561B1 (es) * 2014-06-16 2016-09-30 Universitat De València Nuevos promotores derivados de baculovirus con elevada actividad en sistemas de expresión basados en baculovirus
CN104480143B (zh) * 2014-12-04 2017-07-04 广东华南联合疫苗开发院有限公司 一种表达脊髓灰质炎病毒样颗粒蛋白的载体及脊髓灰质炎病毒样颗粒的制备方法
RU2603056C2 (ru) * 2015-01-26 2016-11-20 Государственное научное учреждение Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Российской академии сельскохозяйственных наук, RU Экспрессирующий плазмидный вектор pet32b(+)asfv/p30e2 для синтеза рекомбинантного белка, состоящего из фрагмента p30 вируса африканской чумы свиней, тиоредоксина и полигистидиновых участков
EP3128006A1 (de) * 2015-08-06 2017-02-08 Consejo Superior de Investigaciones Cientificas (CSIC) Werkzeuge zur komplexen multiproteinexpression in pichia pastoris
AU2016321914B2 (en) * 2015-09-17 2020-09-17 Alternative Gene Expression,S.L. Expression of recombinant proteins in trichoplusia ni pupae
CN106987603B (zh) * 2016-01-20 2018-09-07 中国科学院武汉物理与数学研究所 一种重组腺相关病毒的制备方法
KR101773832B1 (ko) * 2017-06-08 2017-09-01 전정배 명나방류 알, 이를 생산하는 방법, 및 명나방류 알을 이용하여 재조합단백질을 생산하는 방법
CN108776225A (zh) * 2018-05-23 2018-11-09 中国农业科学院兰州兽医研究所 猪细小病毒VLPs抗体检测试剂盒及其制备方法、应用
CN110157687A (zh) * 2019-05-25 2019-08-23 青岛易邦生物工程有限公司 一种犬细小病毒三价亚单位疫苗
EP4031172A4 (de) * 2019-09-16 2024-02-21 Dalu Chen Verfahren zur blockierung einer asv-infektion durch unterbrechung von zellrezeptoren

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010074351A (ko) * 2000-01-25 2001-08-04 김강권 곤충 세포주 형질전환용 발현벡터 및 이를 함유한 항세균단백질 생산용 형질전환체
EP1811027A1 (de) * 2004-10-15 2007-07-25 Hiroshima Industrial Promotion Organization Polynukleotid zur herstellung von rekombinantem protein durch seidenraupen
US20090068703A1 (en) * 2007-09-06 2009-03-12 Yu-Chan Chao Protein expression systems
WO2010025764A1 (en) * 2008-09-02 2010-03-11 Alternative Gene Expression S.L. (Algenex) Insect-derived promoters for foreign proteins expression in insect cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965393A (en) 1997-07-01 1999-10-12 National Institute Of Immunology Method for enhancing foreign gene expression in baculovirus expression vector system
ES2232308B1 (es) 2003-11-12 2006-10-16 Laboratorios Calier, S.A. Dispositivo para la cria de larvas.
JP4597188B2 (ja) 2004-03-09 2010-12-15 アイトゲネシシェ・テヒニーシェ・ホッホシューレ・チューリッヒ 多タンパク質適用のための新規発現ツール
WO2011069562A1 (en) 2009-12-11 2011-06-16 Alternative Gene Expression, S.L. (Algenex) Human papillomavirus like structures produced in a non-fermentative system based on insect larva
WO2012168788A2 (en) 2011-06-10 2012-12-13 Alternative Gene Expression S.L. Recombinant dna elements for the expression of recombinant proteins in a host cell

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010074351A (ko) * 2000-01-25 2001-08-04 김강권 곤충 세포주 형질전환용 발현벡터 및 이를 함유한 항세균단백질 생산용 형질전환체
EP1811027A1 (de) * 2004-10-15 2007-07-25 Hiroshima Industrial Promotion Organization Polynukleotid zur herstellung von rekombinantem protein durch seidenraupen
US20090068703A1 (en) * 2007-09-06 2009-03-12 Yu-Chan Chao Protein expression systems
WO2010025764A1 (en) * 2008-09-02 2010-03-11 Alternative Gene Expression S.L. (Algenex) Insect-derived promoters for foreign proteins expression in insect cells

Non-Patent Citations (17)

* Cited by examiner, † Cited by third party
Title
CROUCH E A ET AL: "Effects of baculovirus transactivators IE-1 and IE-2 on the Drosophila heat shock 70 promoter in two insect cell lines", ARCHIVES OF VIROLOGY ; 2005 AUGUST, OFFICIAL JOURNAL OF THE VIROLOGY DIVISIONOF THE INTERNATIONAL UNION OF MICROBIOLOGICAL SOCIETIES, SPRINGER-VERLAG, VI, vol. 150, no. 8, 2 May 2005 (2005-05-02), pages 1563 - 1578, XP019378571, ISSN: 1432-8798, DOI: 10.1007/S00705-005-0527-8 *
FAN H ET AL: "Construction and immunogenicity of recombinant pseudotype baculovirus expressing the capsid protein of porcine circovirus type 2 in mice", JOURNAL OF VIROLOGICAL METHODS, 2008 JUNE, ELSEVIER BV, NL, vol. 150, no. 1-2, 3 April 2008 (2008-04-03), pages 21 - 26, XP022624769, ISSN: 0166-0934, [retrieved on 20080403], DOI: 10.1016/J.JVIROMET.2008.02.011 *
GOMEZ-CASADO E; GOMEZ-SEBASTIAN S; NÚÑEZ MC; LASA-COVARRUBIAS R; MARTINEZ-PULGARIN S; ESCRIBANO JM: "Insect larvae biofactories as a platform for influenza vaccine production", PROTEIN EXPR PURIF, vol. 79, 2011, pages 35 - 43, XP055015002 *
GOMI S ET AL: "Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus", JOURNAL OF GENERAL VIROLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, SPENCERS WOOD, GB, vol. 80, no. 5, May 1999 (1999-05-01), pages 1323 - 1337, XP002686324, ISSN: 0022-1317 *
LIN XU'AI ET AL: "Baculovirus immediately early 1, a mediator for homologous regions enhancer function in trans", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, vol. 7, no. 1, 10 February 2010 (2010-02-10), pages 32, XP021069061, ISSN: 1743-422X *
MASAHIRO TOMITA ET AL: "A germline transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon", TRANSGENIC RESEARCH, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 16, no. 4, 6 April 2007 (2007-04-06), pages 449 - 465, XP019534548, ISSN: 1573-9368, DOI: 10.1007/S11248-007-9087-X *
NAGAI S ET AL: "Comparative transient expression assay analysis of hycu-hr6- and IE1-dependent regulation of baculovirus gp64 early promoters in three insect cell lines", VIRUS RESEARCH, 2011 AMSTERDAM, NL, vol. 155, no. 1, 17 September 2010 (2010-09-17), pages 83 - 90, XP027574274, ISSN: 0168-1702, [retrieved on 20101224] *
NAGAMINE T ET AL: "Induction of a subnuclear structure by the simultaneous expression of baculovirus proteins, IE1, LEF3, and P143 in the presence of hr", VIROLOGY, 2006 SEPTEMBER, ELSEVIER, AMSTERDAM, NL, vol. 352, no. 2, 15 June 2006 (2006-06-15), pages 400 - 407, XP024896408, ISSN: 0042-6822, [retrieved on 20060901], DOI: 10.1016/J.VIROL.2006.04.034 *
NETTLESHIP, J.E.; ASSENBERG, R.; DIPROSE, J.M.; RAHMAN-HUQ, N.; OWENS, R.J.: "Recent advances in the production of proteins in insect and mammalian cells for structural biology", J. STRUCT. BIOL., vol. 172, 2010, pages 55 - 65, XP027249557 *
OGAWA ET AL: "Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: Production of recombinant human serum albumin", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 128, no. 3, 24 January 2007 (2007-01-24), pages 531 - 544, XP005856750, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2006.10.019 *
PASSARELLI, A. L.; L. K. MILLER: "Three baculovirus genes involved in late and very late gene expression: ie-1, ie-n, and lef-2.", J. VIROL., vol. 67, 1993, pages 2149 - 2158, XP055016120 *
S. KANGINAKUDRU ET AL: "Targeting ie-1 gene by RNAi induces baculoviral resistance in lepidopteran cell lines and in transgenic silkworms", INSECT MOLECULAR BIOLOGY, vol. 16, no. 5, October 2007 (2007-10-01), pages 635 - 644, XP055029022, ISSN: 0962-1075, DOI: 10.1111/j.1365-2583.2007.00753.x *
T. NAGAMINE ET AL: "Focal Distribution of Baculovirus IE1 Triggered by Its Binding to the hr DNA Elements", JOURNAL OF VIROLOGY, vol. 79, no. 1, 13 December 2004 (2004-12-13), pages 39 - 46, XP055079870, ISSN: 0022-538X, DOI: 10.1128/JVI.79.1.39-46.2005 *
V. J. VALDES ET AL: "Using Double-stranded RNA to Prevent in Vitro and in Vivo Viral Infections by Recombinant Baculovirus", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 21, 16 May 2003 (2003-05-16), pages 19317 - 19324, XP055029633, ISSN: 0021-9258, DOI: 10.1074/jbc.M212039200 *
W. KANG: "IE1 and hr facilitate the localization of Bombyx mori nucleopolyhedrovirus ORF8 to specific nuclear sites", JOURNAL OF GENERAL VIROLOGY, vol. 86, no. 11, November 2005 (2005-11-01), pages 3031 - 3038, XP055016117, ISSN: 0022-1317, DOI: 10.1099/vir.0.81270-0 *
X. DAI ET AL.: "The acidic activation domains of the baculovirus transactivators IE1 and IE0 are functional for transcriptional activation in both insect and mammalian cells", JOURNAL OF GENERAL VIROLOGY, vol. 85, no. 3, March 2004 (2004-03-01), pages 573 - 582, XP055080436, ISSN: 0022-1317, DOI: 10.1099/vir.0.19747-0 *
Y. KAWASAKI: "Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins", JOURNAL OF GENERAL VIROLOGY, vol. 85, no. 12, December 2004 (2004-12-01), pages 3575 - 3583, XP055079872, ISSN: 0022-1317, DOI: 10.1099/vir.0.80418-0 *

Also Published As

Publication number Publication date
CA2893578A1 (en) 2014-06-12
ES2554752T1 (es) 2015-12-23
US9879280B2 (en) 2018-01-30
JP6143853B2 (ja) 2017-06-07
ES2611302T3 (es) 2017-05-08
EP2928290B1 (de) 2017-05-31
EP2674438A2 (de) 2013-12-18
WO2014086981A1 (en) 2014-06-12
US20150307899A1 (en) 2015-10-29
EP2928290A1 (de) 2015-10-14
ES2612854T3 (es) 2017-05-19
ES2554752T3 (es) 2017-10-09
JP2015519068A (ja) 2015-07-09

Similar Documents

Publication Publication Date Title
EP2674438A3 (de) Overexpressierendes Baculovirentransactivatorensystem zur Produktion von rekombinanten Proteinen
CL2013003028A1 (es) Acido ribonucleico interferente que reprime la expresión de genes objetivo en especies de insectos, polinucleótido aislado que lo codifica, constructo de adn, célula huésped, composición que comprende al acido ribonucleico interferente; y método para disminuir la expresión de un gen objetivo en plagas de insectos.
EP3950941A3 (de) Dnase-polypeptidvarianten
WO2012168493A3 (en) Baculoviral dna elements for the expression of recombinant proteins in a host insect
EP3091073A3 (de) Polypeptide mit xylanaseaktivität und für diese kodierende polynukleotide
EP3741848A3 (de) Proteasevarianten und dafür codierende polynukleotide
WO2013119302A3 (en) Gh61 polypeptide variants and polynucleotides encoding same
EP3628740A3 (de) Expression von rekombinanten proteinen in der trichoplusia-ni-puppe
EP4223772A3 (de) Optimiertes faktor-viii-gen
EP3279320A3 (de) Gh61-polypeptidvarianten uno polynukleotide zur codierung davon
WO2013076259A3 (en) Polypeptides having lysozyme activity and polynucleotides encoding same
WO2013060819A3 (en) Transient expression vectors, preparation and uses thereof
MX356647B (es) Polipeptidos que tienen actividad de fosfolipasa c y polinucleotidos que codifican los mismos.
SG179075A1 (en) Control of pests in plants
EA201270304A1 (ru) Способы получения шелковой пасты
MX2015000312A (es) Polipeptidos que tienen actividad lipasa y polinucleotidos que los codifican.
EP3321360A3 (de) Alpha-amylase-varianten und polynukleotide zur codierung davon
EP3964575A3 (de) Alpha-amylase-varianten und polynukleotide zur codierung davon
WO2013074956A3 (en) Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
EA201690355A1 (ru) Инсектицидные полипептиды с широким спектром активности и их применения
WO2012030845A3 (en) Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
WO2012175708A3 (en) Polypeptides having protease activity and polynucleotides encoding same
MX2018007677A (es) Operaciones de cosecha mejoradas para proteinas recombinadas.
IN2014CN03187A (de)
WO2010136814A3 (en) Electrodeposition of elemental zirconium

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/866 20060101ALI20140116BHEP

Ipc: C12N 15/85 20060101ALI20140116BHEP

Ipc: C07K 14/005 20060101AFI20140116BHEP

RBV Designated contracting states (corrected)

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

17P Request for examination filed

Effective date: 20140818

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20151109