EP2646126A4 - Affinitätschromatographiematrix - Google Patents
AffinitätschromatographiematrixInfo
- Publication number
- EP2646126A4 EP2646126A4 EP11845161.6A EP11845161A EP2646126A4 EP 2646126 A4 EP2646126 A4 EP 2646126A4 EP 11845161 A EP11845161 A EP 11845161A EP 2646126 A4 EP2646126 A4 EP 2646126A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- affinity chromatography
- chromatography matrix
- matrix
- affinity
- chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
- B01J20/3206—Organic carriers, supports or substrates
- B01J20/3208—Polymeric carriers, supports or substrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3268—Macromolecular compounds
- B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
- B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US41749410P | 2010-11-29 | 2010-11-29 | |
PCT/SE2011/051432 WO2012074463A1 (en) | 2010-11-29 | 2011-11-28 | Affinity chromatography matrix |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2646126A1 EP2646126A1 (de) | 2013-10-09 |
EP2646126A4 true EP2646126A4 (de) | 2014-12-17 |
Family
ID=46172160
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP11845161.6A Withdrawn EP2646126A4 (de) | 2010-11-29 | 2011-11-28 | Affinitätschromatographiematrix |
Country Status (5)
Country | Link |
---|---|
US (1) | US8859726B2 (de) |
EP (1) | EP2646126A4 (de) |
JP (1) | JP2014502272A (de) |
CN (1) | CN103228328A (de) |
WO (1) | WO2012074463A1 (de) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8592555B2 (en) | 2008-08-11 | 2013-11-26 | Emd Millipore Corporation | Immunoglobulin-binding proteins with improved specificity |
SG195555A1 (en) | 2008-12-24 | 2013-12-30 | Emd Millipore Corp | Caustic stable chromatography ligands |
JP5997176B2 (ja) * | 2010-12-21 | 2016-09-28 | ザ ユニバーシティ オブ ウエスタン オンタリオThe University of Western Ontario | プロテインaの新規アルカリ抵抗性変異体及びアフィニティークロマトグラフィーにおけるその使用 |
SG10201604559TA (en) * | 2011-06-08 | 2016-07-28 | Emd Millipore Corp | Chromatography matrices including novel staphylococcus aureus protein a based ligands |
EP3019520B1 (de) * | 2013-07-10 | 2019-12-11 | GE Healthcare BioProcess R&D AB | Mutierte immunglobulinbindende polypeptide |
CA2920005C (en) * | 2013-08-28 | 2023-11-28 | Affibody Ab | Binding polypeptides having a mutated scaffold |
CN104513309A (zh) * | 2013-09-26 | 2015-04-15 | 上海亨臻实业有限公司 | 一种蛋白功能结构域铰链肽及其用途 |
US11566082B2 (en) * | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
GB201506868D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method for protein purification |
US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
EP3455241B1 (de) | 2016-05-11 | 2022-02-23 | Cytiva BioProcess R&D AB | Verfahren zur reinigung und/oder sanierung einer trennmatrix |
WO2017194592A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Method of storing a separation matrix |
US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
US10513537B2 (en) | 2016-05-11 | 2019-12-24 | Ge Healthcare Bioprocess R&D Ab | Separation matrix |
CN106117324A (zh) * | 2016-07-29 | 2016-11-16 | 湖北爱晟生物科技有限公司 | 重组蛋白a及其编码基因和应用 |
GB201703116D0 (en) * | 2017-02-27 | 2017-04-12 | Ge Healthcare Bioprocess R&D Ab | A seperation matrix and a method of seperating antibodies |
GB2569585A (en) * | 2017-12-20 | 2019-06-26 | Ge Healthcare Bio Sciences Ab | A method for preparation of a separation matrix |
CN109721645A (zh) * | 2017-12-29 | 2019-05-07 | 兆生生物科技南京有限公司 | 一种基因突变的蛋白a及其应用 |
EP3650465A1 (de) * | 2018-11-12 | 2020-05-13 | Navigo Proteins Gmbh | Neuartige populationen von polypeptiden mit einer dreifachhelixstruktur |
US20220251147A1 (en) * | 2019-04-02 | 2022-08-11 | Bondwell Technologies Inc. | Functionalized ubx protein materials for enhanced purification of antibodies |
GB202113626D0 (en) | 2021-09-24 | 2021-11-10 | Cytiva Bioprocess R & D Ab | FC binding polypeptides |
CN113999294B (zh) * | 2021-12-30 | 2022-03-29 | 苏州纳微科技股份有限公司 | 免疫球蛋白结合蛋白及其应用 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5362859A (en) * | 1992-07-27 | 1994-11-08 | Sepracor, Inc. | High-capacity affinity supports and methods for the preparation and use of same |
SE0104353D0 (sv) * | 2001-12-19 | 2001-12-19 | Amersham Biosciences Ab | Separation method |
SE0200943D0 (sv) * | 2002-03-25 | 2002-03-25 | Amersham Biosciences Ab | Mutant protein |
US7709209B2 (en) | 2002-03-25 | 2010-05-04 | Ge Healthcare Bio-Sciences Ab | Protein ligands |
SE0400274D0 (sv) * | 2004-02-09 | 2004-02-09 | Affibody Ab | New polypeptide |
JP5345539B2 (ja) * | 2006-09-29 | 2013-11-20 | ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー | 抗体単離のためのスタフィロコッカスアウレウス由来のドメインcを含むクロマトグラフィーリガンド |
CN101224414A (zh) * | 2007-10-18 | 2008-07-23 | 西北大学 | 以四唑为功能基的多用途分离介质及其制备方法 |
JP5229888B2 (ja) * | 2008-09-30 | 2013-07-03 | 独立行政法人産業技術総合研究所 | 弱酸性域での易解離性を向上したプロテインa変異型タンパク質及び抗体捕捉剤 |
JP2012515160A (ja) | 2009-01-12 | 2012-07-05 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | アフィニティークロマトグラフィーマトリックス |
-
2011
- 2011-11-28 JP JP2013541958A patent/JP2014502272A/ja active Pending
- 2011-11-28 WO PCT/SE2011/051432 patent/WO2012074463A1/en active Application Filing
- 2011-11-28 US US13/990,135 patent/US8859726B2/en active Active
- 2011-11-28 EP EP11845161.6A patent/EP2646126A4/de not_active Withdrawn
- 2011-11-28 CN CN2011800565779A patent/CN103228328A/zh active Pending
Non-Patent Citations (3)
Title |
---|
GÜLICH S ET AL: "Protein engineering of an IgG-binding domain allows milder elution conditions during affinity chromatography", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 76, 1 January 2000 (2000-01-01), pages 233 - 244, XP002903869, ISSN: 0168-1656 * |
LINHULT MARTIN ET AL: "Improving the tolerance of a protein a analogue to repeated alkaline exposures using a bypass mutagenesis approach", PROTEINS: STRUCTURE, FUNCTION, AND BIOINFORMATICS, JOHN WILEY & SONS, INC, US, vol. 55, no. 2, 1 May 2004 (2004-05-01), pages 407 - 416, XP002488610, ISSN: 0887-3585, DOI: 10.1002/PROT.10616 * |
See also references of WO2012074463A1 * |
Also Published As
Publication number | Publication date |
---|---|
US20130245224A1 (en) | 2013-09-19 |
CN103228328A (zh) | 2013-07-31 |
US8859726B2 (en) | 2014-10-14 |
EP2646126A1 (de) | 2013-10-09 |
WO2012074463A1 (en) | 2012-06-07 |
JP2014502272A (ja) | 2014-01-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP2654915A4 (de) | Affinitätschromatographiematrix | |
EP2654914A4 (de) | Affinitätschromatographiematrix | |
EP2646126A4 (de) | Affinitätschromatographiematrix | |
EP2389386A4 (de) | Affinitätschromatographiematrix | |
EP2831096A4 (de) | Affinitätschromatografiematrix | |
HK1203435A1 (en) | Fc-receptor based affinity chromatography fc- | |
EP2622447A4 (de) | Anwendungsverhalten auf zwei bildschirmen | |
EP2622464A4 (de) | Computer für berechnungen in verschiedenen umgebungen | |
PL2464655T3 (pl) | Procesy chromatograficzne | |
IL243414A0 (en) | Affinity chromatography matrix | |
ZA201305583B (en) | Support assembly | |
EP2562178A4 (de) | Füllmittel für affinitätschromatographie | |
EP2611291A4 (de) | Perfusionszusammensetzung | |
GB201020148D0 (en) | Chromatography column | |
PT3915659T (pt) | Suporte de afinidade | |
EP2590722A4 (de) | Zeitmessverfahren | |
GB2497004B (en) | Cylinder block assembly for x-engines | |
GB2478652B (en) | Chromatography assembly | |
EP2660453A4 (de) | Kolben | |
PL2363542T3 (pl) | Zespół sterujący | |
GB201020146D0 (en) | Chromatography column | |
EP2611453A4 (de) | Antimykotische zusammensetzung | |
EP2582607A4 (de) | Bremsanordnung | |
PL2434173T3 (pl) | Zespół hamulca | |
GB2480426B (en) | Support assembly |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20130515 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20141117 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: B01J 20/286 20060101ALI20141111BHEP Ipc: B01J 20/32 20060101ALI20141111BHEP Ipc: B01D 15/38 20060101AFI20141111BHEP Ipc: C07K 16/06 20060101ALI20141111BHEP Ipc: C07K 1/22 20060101ALI20141111BHEP Ipc: B01J 20/281 20060101ALI20141111BHEP Ipc: C12N 15/03 20060101ALI20141111BHEP Ipc: C07K 14/31 20060101ALI20141111BHEP |
|
17Q | First examination report despatched |
Effective date: 20141212 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20150423 |