EP2635892B1 - Spectroscopic finger-printing of raw material - Google Patents

Spectroscopic finger-printing of raw material Download PDF

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Publication number
EP2635892B1
EP2635892B1 EP11778872.9A EP11778872A EP2635892B1 EP 2635892 B1 EP2635892 B1 EP 2635892B1 EP 11778872 A EP11778872 A EP 11778872A EP 2635892 B1 EP2635892 B1 EP 2635892B1
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Prior art keywords
spectra
cultivation
different
lots
lot
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German (de)
English (en)
French (fr)
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EP2635892A1 (en
Inventor
José CARDOSO-MENEZES
Christian Hakemeyer
Gledson Emidio Jose
Ulrike Strauss
Silke Werz
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
    • G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
    • G01N24/087—Structure determination of a chemical compound, e.g. of a biomolecule such as a protein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/129—Using chemometrical methods
    • G01N2201/1293—Using chemometrical methods resolving multicomponent spectra

Definitions

  • Table 3 Analogously the influence of rice protein hydrolyzate on process performance can be evaluated (Table 3).
  • Table 3 batch soy protein hydrolyzate lot No. chemically defined basic medium lot No .
  • the three analyzed cultivation media components show significant lot-to-lot variability in granularity and humidity content, as can be seen by the NIR spectra obtained. NIR is very sensitive to both these factors. Additionally both these factors dominate over smaller but still significant chemical composition differences that might be present. Prior to PCA analysis physical information has to be removed by spectra pre-processing.
  • fluorescence excitation-emission spectra preferably acquired of different water soluble fermentation raw-materials.
  • a three-way data array, with excitation wavelengths along the x-axis, emission wavelengths along the y-axis, and intensity along the z-axis can be established.
  • Figure 8 a fluorescence EEM landscape of a soy protein hydrolyzate lot samples is shown.
  • a typical EEM spectrum can be influenced by Rayleigh and Raman scattering effects, which affect the information content of the fluorescence landscape. To overcome the Rayleigh effect several strategies and techniques can be used:
  • This region (200 nm to 225 nm) was excluded from the spectra, as well the non-informative emission wavelengths (200 nm to 315 nm and 596 nm to 600 nm) and excitation wavelengths (580 nm to 600 nm).
  • the resulting spectrum is shown in Figure 9 .
  • a PCA of the unfolded fluorescence data array can be carried out for each component raw material.
  • the unfolding procedure can be applied in any of the three modes of a three-way array.
  • the unfolding preserving information of the first mode can be employed. In this way, the fluorescence landscapes can be unfolded into a row of emission spectra one after the other ( Figure 10 ).
  • the dimensions of the soy protein hydrolyzate array are 19x138x70 (lot x emission wavelength x excitation wavelength). After the unfolding strategy, a two-way matrix of size 19x9,960 can be obtained. Figure 11 shows a small part of the resulting spectra for three different lots of soy protein hydrolyzate. Noise in the extreme excitation wavelengths can be seen.
  • FIG. 14 shows the score plot of PC1 x PC2 of a PCA using two principal components covering more than 92 % of the total variance in the unfolded EEM spectra. As before with NIR spectra for the same media components it was found that lots giving higher yields are separated from lots giving lower yields in the PCA score plots of EEM unfolded spectra.
  • the obtained model was made up of only two LVs but a non-significant R 2 of 0.139 was obtained.
  • the measured vs. cross-validation predicted plot is presented in Figure 15 .
  • a PLS model correlating NIR spectra of different lots of the chemically defined basic medium and product yield can be built using the calibration dataset as presented in Table 8.
  • Table 8. batch chemically defined basic medium F/ZF lot No. product at 330h [mg/l] D45KD11 1 1314 D52KD13 2 1458 D61KD12 3 1134 D73KD21 4 1147 D79KD22 5 1162
  • the obtained model was made up of only two LVs but again a non significant R 2 of 0.04 was obtained ( Figure 16 ).
  • a combination strategy can be used between same spectroscopic/different media components and also between different spectroscopic/different media components.
  • the method as reported herein is directed to the combination of spectra of different nature (fluorescence spectra and IR spectra), which intrinsically have different dimensions (two (2D) and one (1D), respectively), and that requires the operations of first compressing each spectrum to principal component analysis scores and second producing linear combinations of each spectrum scores.
  • the spectra of different nature are combined by means of a dimensional reduction and a linear combination of those reduced transformed variables (PCA scores obtained by compressing each spectrum).
  • spectra of different dimensions and nature are used to capture in a mixture of two different fermentation raw materials the components responsible for fermentation performance of said raw materials and to make predictions of fermentation yields for a specific combination of lots.
  • NIR NIR emerges in 1960s into the analytical world, with the work of Karl Norris of the US Department of Agriculture (Siesler et al, 2002). In the electromagnetic spectrum, the NIR region is located in between Mid-Infrared and Visible. In a range of wavenumber 4,000-14,000 cm -1 (respectively wavelength 700-2,500 nm), the absorption radiation of overtone and combination bands of covalent bonds such as N-H, O-H and C-H of organic molecules ( Figure 21 ).
  • Fluorescence spectroscopy uses irradiation at a certain wavelength to excite molecules, which will then emit radiation of a different wavelength. This technique is often used for studying the structure and function of macromolecules, especially protein interactions. Tentative assignment of fluorescence characteristics of chromophores found in proteins and nucleic acids is presented in the following Table.
  • the major problems are related to the Raman and Rayleigh scattering, which are caused by deviations of the light that are not related to the fluorescence properties of the sample. Since the wavelength regions affected by scattering are known, the intensities measured in such particular regions can be removed replacing it by interpolated points.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biomedical Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
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  • Medicinal Chemistry (AREA)
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  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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EP11778872.9A 2010-11-05 2011-11-03 Spectroscopic finger-printing of raw material Active EP2635892B1 (en)

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JP (1) JP5683713B2 (ja)
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CA (1) CA2815612C (ja)
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CN105424634A (zh) * 2015-10-29 2016-03-23 中国计量学院 一种基于光纤耦合紫外光源的水质cod检测器及其预测模型优化系统
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CN107941745A (zh) * 2017-11-16 2018-04-20 赣州市检验检疫科学技术研究院 基于近红外光谱鉴别染色橙的方法
CN108120696A (zh) * 2017-12-18 2018-06-05 福建中医药大学 一种不同栽培方式的大圆叶金线莲的鉴别方法
CN108169167A (zh) * 2017-12-18 2018-06-15 福建中医药大学 一种不同栽培方式的台湾金线莲的鉴别方法
CN108132224A (zh) * 2017-12-18 2018-06-08 福建中医药大学 一种不同栽培方式的金线莲的鉴别方法
CN108169166A (zh) * 2017-12-18 2018-06-15 福建中医药大学 一种不同栽培方式的尖叶金线莲的鉴别方法
CN108152245A (zh) * 2017-12-18 2018-06-12 福建中医药大学 一种金线莲及其混伪品的鉴别方法
CN108132223A (zh) * 2017-12-18 2018-06-08 福建中医药大学 一种不同栽培方式的红霞金线莲的鉴别方法
GB201806752D0 (en) * 2018-04-25 2018-06-06 Ge Healthcare Bioprocess R&D Ab Method in bioprocess system
JP7190103B2 (ja) * 2018-09-03 2022-12-15 株式会社サタケ 米の産地判別方法
KR20210022319A (ko) 2019-08-20 2021-03-03 삼성전자주식회사 생체정보 추정 장치 및 방법
WO2021049044A1 (ja) * 2019-09-13 2021-03-18 エピストラ株式会社 培地製造方法、培地製造パラメータ決定方法、培地、およびプログラム
FR3103900B1 (fr) * 2019-11-29 2024-07-19 Univ Du Mans Méthode d'identification rapide de microorganismes par analyse de matrices excitation-émission
JP6977977B1 (ja) * 2020-06-24 2021-12-08 エピストラ株式会社 培地の異常検知装置及び異常検知方法

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CN103201616B (zh) 2015-11-25
MX2013004882A (es) 2013-07-02
JP5683713B2 (ja) 2015-03-11
CA2815612A1 (en) 2012-05-10
EP2635892A1 (en) 2013-09-11
US20140032127A1 (en) 2014-01-30
RU2013123903A (ru) 2014-12-10
MX341795B (es) 2016-09-02
US10816477B2 (en) 2020-10-27
ES2506390T3 (es) 2014-10-13
KR101507252B1 (ko) 2015-03-30
US20180202938A1 (en) 2018-07-19
RU2593005C2 (ru) 2016-07-27
BR112013010993A2 (pt) 2016-08-23
JP2013544353A (ja) 2013-12-12
WO2012059520A1 (en) 2012-05-10
CA2815612C (en) 2019-01-08
BR112013010993B1 (pt) 2020-02-18
HK1187110A1 (zh) 2014-03-28
CN103201616A (zh) 2013-07-10
KR20130079571A (ko) 2013-07-10

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