EP2616481A2 - Production biologique de produits chimiques aromatiques à partir de composés dérivés de lignine - Google Patents

Production biologique de produits chimiques aromatiques à partir de composés dérivés de lignine

Info

Publication number
EP2616481A2
EP2616481A2 EP11825655.1A EP11825655A EP2616481A2 EP 2616481 A2 EP2616481 A2 EP 2616481A2 EP 11825655 A EP11825655 A EP 11825655A EP 2616481 A2 EP2616481 A2 EP 2616481A2
Authority
EP
European Patent Office
Prior art keywords
lignin
daltons
polypeptide
amino acid
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11825655.1A
Other languages
German (de)
English (en)
Other versions
EP2616481A4 (fr
Inventor
Ranjini Chatterjee
Kenneth Zahn
Kenneth Mitchell
Gary Y. Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aligna Technologies Inc
Original Assignee
Aligna Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aligna Technologies Inc filed Critical Aligna Technologies Inc
Publication of EP2616481A2 publication Critical patent/EP2616481A2/fr
Publication of EP2616481A4 publication Critical patent/EP2616481A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01018—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C12P7/28—Acetone-containing products

Abstract

L'invention concerne de manière générale un procédé de conversion de composés dérivés de lignine en produits chimiques aromatiques de valeur au moyen d'un procédé de conversion biologique enzymatique. L'invention concerne une sélection (i) de cellules hôtes qui sont tolérantes aux composés toxiques présents dans les fractions de lignine ; (ii) de polypeptides qui peuvent être utilisés comme enzymes dans la conversion biologique des fractions de lignine en produits chimiques aromatiques ; (iii) de polynucléotides qui peuvent être utilisés pour transformer les cellules hôtes afin qu'elles expriment la sélection de polypeptides en tant qu'enzymes dans la conversion biologique des fractions de lignine ; et (iv) de transformants qui expriment les enzymes.
EP11825655.1A 2010-09-15 2011-08-29 Production biologique de produits chimiques aromatiques à partir de composés dérivés de lignine Withdrawn EP2616481A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40340010P 2010-09-15 2010-09-15
US45570910P 2010-10-25 2010-10-25
PCT/US2011/049619 WO2012036884A2 (fr) 2010-09-15 2011-08-29 Production biologique de produits chimiques aromatiques à partir de composés dérivés de lignine

Publications (2)

Publication Number Publication Date
EP2616481A2 true EP2616481A2 (fr) 2013-07-24
EP2616481A4 EP2616481A4 (fr) 2014-04-02

Family

ID=45832163

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11825655.1A Withdrawn EP2616481A4 (fr) 2010-09-15 2011-08-29 Production biologique de produits chimiques aromatiques à partir de composés dérivés de lignine

Country Status (6)

Country Link
EP (1) EP2616481A4 (fr)
JP (1) JP2014506115A (fr)
CN (1) CN103797026A (fr)
AU (1) AU2011302522A1 (fr)
CA (1) CA2811403A1 (fr)
WO (1) WO2012036884A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014200509A1 (fr) * 2013-06-14 2014-12-18 Washington State University Research Foundation Conversion de lignine en composés phénoliques et carboxylates
JP6459514B2 (ja) 2013-07-09 2019-01-30 東レ株式会社 糖液の製造方法
WO2018195000A1 (fr) * 2017-04-17 2018-10-25 Board Of Trustees Of Michigan State University Procédés de dépolymérisation de lignine à l'aide de thiols
WO2018204424A1 (fr) * 2017-05-01 2018-11-08 National Technology & Engineering Solutions Of Sandia, Llc Nouvelles compositions et procédés de synthèse de solvants eutectiques profonds à partir de composés phénoliques dérivés de lignine
CN109423456B (zh) * 2017-08-30 2022-08-19 中国石油化工股份有限公司 一种圆褐固氮菌及其鉴定方法和应用
KR101831966B1 (ko) 2017-10-27 2018-02-23 경상대학교산학협력단 휴믹화된 리그닌 전환체의 생산 방법
CN117285663A (zh) * 2023-10-17 2023-12-26 中国石油大学(华东) 一种木质纤维生物质组分温和梯级分离的方法

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US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
ZA811368B (en) 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
NZ201705A (en) 1981-08-31 1986-03-14 Genentech Inc Recombinant dna method for production of hepatitis b surface antigen in yeast
US4943529A (en) 1982-05-19 1990-07-24 Gist-Brocades Nv Kluyveromyces as a host strain
AU2353384A (en) 1983-01-19 1984-07-26 Genentech Inc. Amplification in eukaryotic host cells
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
AU3145184A (en) 1983-08-16 1985-02-21 Zymogenetics Inc. High expression of foreign genes in schizosaccharomyces pombe
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
US4946783A (en) 1987-01-30 1990-08-07 President And Fellows Of Harvard College Periplasmic protease mutants of Escherichia coli
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
GB8724885D0 (en) 1987-10-23 1987-11-25 Binns M M Fowlpox virus promotors
ATE105585T1 (de) 1987-12-21 1994-05-15 Univ Toledo Transformation von keimenden pflanzensamen mit hilfe von agrobacterium.
AU4005289A (en) 1988-08-25 1990-03-01 Smithkline Beecham Corporation Recombinant saccharomyces
DE68927344T2 (de) 1989-04-28 1997-02-20 Rhein Biotech Proz & Prod Gmbh Hefezellen der Gattung-Schwanniomyces
FR2646437B1 (fr) 1989-04-28 1991-08-30 Transgene Sa Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
EP0402226A1 (fr) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Vecteurs de transformation de la levure yarrowia
FR2649120B1 (fr) 1989-06-30 1994-01-28 Cayla Nouvelle souche et ses mutants de champignons filamenteux, procede de production de proteines recombinantes a l'aide de ladite souche et souches et proteines obtenues selon ce procede
JP2002034557A (ja) * 2000-07-18 2002-02-05 Rengo Co Ltd リグニンの主要な単位間結合であるアリールグリセロール−β−アリールエーテル型結合の切断酵素、その製造方法およびその生産微生物

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
E. MASAI ET AL: "Roles of the Enantioselective Glutathione S-Transferases in Cleavage of -Aryl Ether", JOURNAL OF BACTERIOLOGY, vol. 185, no. 6, 15 March 2003 (2003-03-15), pages 1768-1775, XP055101271, ISSN: 0021-9193, DOI: 10.1128/JB.185.6.1768-1775.2003 *
MASAI ET AL: "Characterization of Sphingomonas paucimobilis SYK-6 genes involved in degradation of lignin-related compounds.", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 23, no. 4-5, 1 October 1999 (1999-10-01), pages 364-373, XP055101268, ISSN: 1367-5435, DOI: 10.1038/sj/jim/2900747 *
See also references of WO2012036884A2 *

Also Published As

Publication number Publication date
JP2014506115A (ja) 2014-03-13
CA2811403A1 (fr) 2012-03-22
WO2012036884A3 (fr) 2012-08-02
CN103797026A (zh) 2014-05-14
EP2616481A4 (fr) 2014-04-02
AU2011302522A1 (en) 2013-05-02
WO2012036884A2 (fr) 2012-03-22

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