EP2532426A2 - Cartridge, centrifuge and method - Google Patents
Cartridge, centrifuge and method Download PDFInfo
- Publication number
- EP2532426A2 EP2532426A2 EP20120163519 EP12163519A EP2532426A2 EP 2532426 A2 EP2532426 A2 EP 2532426A2 EP 20120163519 EP20120163519 EP 20120163519 EP 12163519 A EP12163519 A EP 12163519A EP 2532426 A2 EP2532426 A2 EP 2532426A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- drum
- actuator
- chamber
- cartridge
- central axis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F29/00—Mixers with rotating receptacles
- B01F29/15—Use of centrifuges for mixing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F29/00—Mixers with rotating receptacles
- B01F29/30—Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
- B01F29/32—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor
- B01F29/321—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor of test-tubes or the like
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/42—Mixers with shaking, oscillating, or vibrating mechanisms with pendulum stirrers, i.e. with stirrers suspended so as to oscillate about fixed points or axes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
- B01F33/453—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements
- B01F33/4533—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements supporting the stirring element in one point
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/713—Feed mechanisms comprising breaking packages or parts thereof, e.g. piercing or opening sealing elements between compartments or cartridges
- B01F35/7137—Piercing, perforating or melting membranes or closures which seal the compartments
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/716—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components
- B01F35/7161—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components the containers being connected coaxially before contacting the contents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
- B01F35/71725—Feed mechanisms characterised by the means for feeding the components to the mixer using centrifugal forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0841—Drums
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
Definitions
- biochemical processes are based in particular on the handling of liquids. Typically, this manipulation is done manually with tools such as pipettes, reaction vessels, active probe surfaces or laboratory equipment. By pipetting robots or special equipment, these processes are already partially automated.
- Lab-on-a-Chip systems (also referred to as the West Pocket Laboratory or the Chiplabor) accommodate all the functionality of a macroscopic laboratory on a plastic plastic-sized plastic substrate.
- Lab-on-a-chip systems typically consist of two major components.
- a test carrier includes structures and mechanisms for the implementation of basic fluidic operations (e.g., mixers), which may consist of passive components such as channels, reaction chambers and upstream reagents, or even active components such as valves or pumps.
- the second main component is actuation, detection and control units.
- Such systems make it possible to carry out biochemical processes fully automatically.
- a lab-on-a-chip system is for example in the document DE 10 2006 003 532 A1 described.
- This system comprises a rotor chip, which is rotatably provided with respect to a stator chip.
- the rotor chip can be coupled by means of fluidic channels with the stator chip for filling or emptying the rotor chip.
- the cartridge defined in claim 1, the centrifuge defined in claim 13 and the method defined in claim 15 have the advantage over conventional solutions that the processing of at least one component in the cartridge can be carried out independently of the rotational speed of the centrifuge.
- the first and second chambers or the first and third chambers can be conductively connected to one another by actuation of the actuator.
- the operation can still be supported by the centrifugal force.
- the first chamber is conductively connected to the second chamber, and the first component is thereafter transferred from the second chamber to the first chamber due to the action of the centrifugal force. Thereafter, the first chamber is in turn connected by actuation of the actuator with the third chamber, and the second component thereafter also flows into the first chamber, for example in order to be mixed with the first component.
- the first chamber is first conductively connected to the second chamber and filled a certain proportion of the component in the second chamber. Thereafter, the first chamber is conductively connected to the third chamber and filled a further portion of the component in the third chamber.
- the first and third chambers or the second and third chambers can be conductively connected to one another by actuation of the actuator.
- the operation can still be supported by the centrifugal force.
- the second variant at least one first component in the first chamber and a second component in the second chamber held.
- the first chamber is conductively connected to the third chamber, and the first component is thereafter transferred from the first chamber to the third chamber due to the action of the centrifugal force.
- the second chamber is in turn connected by actuation of the actuator with the third chamber, and the second component thereafter flows into the third chamber, for example, to be mixed with the first component.
- the first chamber is first conductively connected to the third chamber and filled a certain proportion of the component in the first chamber.
- the second chamber is conductively connected to the third chamber and filled a further portion of the component in the second chamber.
- the first and second variants can also be combined.
- Component in this case means a liquid, a gas or a particle.
- chamber is presently preferably a line section, which is formed on both sides or only open on one side, as well as a substantially closed space meant, which has an inlet and / or outlet.
- first chamber with either the second or with the third chamber (first variant) or either the first chamber with the third chamber or the second chamber with the third chamber (second variant) in dependence is connected by an actuation by the actuator.
- the actuator is provided electrically, mechanically and / or pressure-operated.
- a piezoelectrically, electrostatically, semi-mechanically manually or electromagnetically operated actuator is suitable.
- Printing companies means that the actuator operates the first drum by utilizing a gas or liquid pressure.
- the actuator has an actuator which is connected to the first drum in order to rotate it directly and if necessary to move along the central axis.
- the actuator has an actuator, which is connected to the first drum to move along the central axis and thereby rotate.
- the actuator actuates the ballpoint pen mechanism described below, whereupon the ballpoint pen mechanism rotates the first drum.
- the first drum is thus indirectly operated.
- the adjusting device comprises a first bevel, which cooperates with a second bevel of the first drum, in order from a first position in which this is a form-locking engagement with a housing of the cartridge in the rotational direction about the central axis in a second position along the central axis to spend, in which the positive connection is canceled and the first drum rotates about the central axis due to the action of a return means or the actuator or another actuator.
- a ballpoint pen mechanism is provided.
- the actuator actuates the first slope for cooperation with the second slope.
- the actuator moves the first slope in a direction away from the pivot point of the cartridge of the centrifuge along the central axis.
- the first drum is a second drum upstream or downstream relative to the central axis, wherein the actuator actuates the second drum for rotating the first drum.
- the first drum is thus actuated indirectly by means of the second drum.
- the actuator abuts flat against the first and / or second drum for pressing against it, or the actuator is fixedly connected to the first or second drum.
- the configuration according to which the actuator abuts flat against the first and / or second drum for pressing against it, is characterized by a simple construction and a simple assembly.
- the fixed connection between the first or second drum and the actuator has the advantage that the actuator can move the first or second drum in opposite directions.
- the actuator is arranged between the first and second drum.
- the actuator is preferably designed as a piezoelectric element.
- the cartridge has a housing which is closed at one end by means of an adapter, wherein the actuator is attached to the adapter.
- the actuator is integrated in the adapter.
- the adapter has a flexible membrane which can be actuated on its one side by means of the actuator and acts on its other side on the first drum or the second drum. This can create a more sterile outcome.
- the actuator is thus preferably outside the interior of the housing.
- the second and / or third chamber of the first drum upstream or downstream relative to the central axis and / or is formed in the second drum.
- a second chamber of the first drum and a third chamber of the first drum may be downstream.
- the second chamber is provided in the second drum and the third chamber is provided in a third drum.
- the actuator is arranged in front of or behind the first drum with respect to a pivot point of the cartridge in the centrifuge. If the actuator is arranged behind the first drum with respect to the fulcrum, it is advantageous that no other return means is required or this is formed by the actuator itself.
- the actuation of the first drum is effected by the actuator in cooperation with the centrifugal force.
- FIG. 1 shows in a sectional view a cartridge 100 according to an embodiment of the present invention.
- the cartridge 100 includes a housing 102 in the form of a tube.
- the housing 102 may be formed as a 15 mL centrifuge tube, 1.5 mL or 2 mL Eppendorf tube, or alternatively as a microtiter plate (e.g., 20 ⁇ L per well).
- the longitudinal axis of the housing 102 is designated 104.
- a first drum 108, a second drum 106 and a third drum 110 are accommodated in the housing 102.
- the drums 106, 108, 110 are arranged one behind the other and with their respective central axes coaxial with the longitudinal axis 104.
- the housing 102 is formed closed at its one end 112. Between the closed end 112 and the drum 110 arranged adjacent thereto, a return means, for example in the form of a spring 114, is arranged.
- the spring 114 may be in the form of a coil spring or a polymer, in particular an elastomer.
- the other end 116 of the housing 102 is closed by means of a closure 118.
- the closure 118 may be removed to remove the drums 106, 108, 110 from the housing 102.
- the housing 102 itself can be dismantled to remove the drums 106, 108, 110 or to reach the chambers, for example the chamber 136.
- the spring 114 is disposed between the shutter 118 and the drum 106 so that the spring 114 is stretched to generate a restoring force.
- Other arrangements of the spring 114 are conceivable.
- a respective drum 106, 108, 110 may have one or more chambers:
- the second drum 106 includes a plurality of reagent chambers 120 and another chamber 122 for receiving a sample, such as a blood sample, taken from a patient.
- a sample such as a blood sample
- the first drum 108 connected downstream of the second drum 106 comprises a mixing chamber 124, in which the reagents from the chambers 120 with the sample the chamber 122 are mixed.
- the first drum 108 includes, for example, a chamber 126 in which the mixture from the mixing chamber 124 is separated into a liquid and a solid phase 128 and 130, respectively.
- the solid phase 130 may be a gel column, a silica matrix, or a filter.
- the third drum 110 which is in turn connected downstream of the first drum 108, comprises a chamber 132 for receiving a waste product 134 from the chamber 126. Furthermore, the third drum 110 comprises a further chamber 136 for receiving the desired end product 138.
- the cartridge 100 has an outer geometry so that it can be used in a receptacle of a rotor of a centrifuge, in particular in a receptacle of a swing-bucket rotor or fixed-angle rotor of a centrifuge.
- the cartridge 100 is moved by one in FIG. 1 schematically indicated pivot point 140 rotated at high speed.
- the pivot point 140 lies on the longitudinal axis 104, so that a corresponding centrifugal force 142 along the longitudinal axis 104 acts on each component of the cartridge 100.
- the mixing chamber 124 is first to be fluidly connected to the chamber 122 to receive the sample from the chamber 122. Thereafter, the mixing chamber 124 is to be connected to the chambers 120 to receive the reagents from these. Subsequently, the reagents and the sample are to be mixed in the mixing chamber 124. Similarly, the processes in chambers 126, 132 and 136 should also be controlled. In addition to the control of the processes by means of the actuator 139, the rotational speed of the centrifuge or a rotor thereof can be controlled accordingly.
- the acting centrifugal force 142 has, in particular, the task of transferring the components, for example the sample or the reagents, between the chambers 120, 122 and the mixing chamber 124.
- FIG. 2A-2G perspective view of various components of the cartridge 100 from FIG. 1 , Based on Figures 2A-2G in particular an adjusting device 300 (see Fig. 3A ) comprising the actuator 139, which enables the control of the above-mentioned processes.
- the housing 102 on its inside projections 200.
- the projections 200 are radially from the housing inner wall 202 toward the longitudinal axis 104.
- the projections 200 form between them slots 204 which extend along the longitudinal axis 104.
- the projections 200 are formed at their one end in each case with a slope 206.
- the slopes 206 face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
- FIG. 2B shows the end 112 of the housing 102, which is formed according to this embodiment as a removable cap.
- the end 112 has at its inner periphery a plurality of grooves 208 which extend along the longitudinal axis 104.
- FIG. 2C shows the second drum 106 with the chambers 120, 122.
- the drum 106 has on its outer wall 210 a plurality of projections 212 which extend from the outer wall 210 radially outwardly.
- the projections 212 of the drum 106 engage in the slots 204 of the housing 102.
- a rotation of the drum 106 is locked about the longitudinal axis 104.
- the drum 106 is slidable along the longitudinal axis 104 in the slots 204.
- the second drum 106 furthermore has on its outer wall 210, in particular on its end 214 facing the first drum 108, a crown-like contour 216 which comprises a multiplicity of bevels 218, 220.
- Two bevels 218, 220 each form a point of the crown-like contour 216.
- the ramps 218, 220 also face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
- FIG. 2D shows a view of the second drum 106 from Figure 2C from underneath.
- the underside 222 of the drum 106 assigned to the end 214 has a plurality of openings 224 in order to connect the chambers 120, 122 to the mixing chamber 124 of the first drum 108 in liquid, gas and / or particle form (hereinafter "conductive").
- the openings 224 may also conductively connect the chambers 120, 122 to the chamber 126 of the first drum 108.
- a respective conductive connection is determined by the position of a respective opening 224 with respect to the chambers 124, 126. This position is achieved by rotating the first drum 108 relative to the second drum 106, as will be explained in more detail later.
- FIG. 2E shows a lancing device 226, which in FIG. 1 not shown.
- Lancing device 226 includes a plate 228 having one or more spikes 230 disposed adjacent to an opening 232 in plate 228, respectively.
- the mandrels 230 serve to pierce a respective opening 224 in the underside 222 of the second drum 106 by means of suitable control by the actuator 139, whereupon in particular liquid from the corresponding chamber 120, 122 flows through the opening 232 into the chambers 124 or 126 ,
- Figure 2F shows the first drum 108 with the chambers 124, 126.
- the first drum 108 has a plurality of projections 240 on its outer wall 238.
- the protrusions 240 are configured to engage the slots 204 (as well as the protrusions 212 of the second drum 106). As long as the projections 240 are engaged with the slots 240, rotation of the first drum 108 about the longitudinal axis 104 is disabled. However, the projections 240 together with the drum 108 are movable along the longitudinal axis 104 in the slots 204.
- the projections 240 have bevels 242, which point in the direction of the pivot point 140 during operation of the centrifuge with the cartridge 100 and are formed corresponding to the bevels 206 and 220.
- FIG. 2G shows the third drum 110 with the chambers 132, 136.
- the drum 110 has projections 244 which project from the outer wall 246 of the drum 110 respectively.
- the projections 244 are adapted to engage the grooves 208 of the end 112 so that the drum 110 is displaceable in the longitudinal direction 104 in the grooves 208. A rotation of the drum 110 about the longitudinal axis 104 is thus locked.
- FIG. 3A-3E show several operating conditions during operation of the cartridge 100 FIG. 1 , wherein an additional drum 302 is shown, but this is not relevant in the present case.
- the Figures 4A-4E correspond respectively with the Figures 3A-3E and illustrate the movement of the ramps 206, 218, 220, 242 relative to each other.
- FIG. 3B shows an operating state of the cartridge 100 which is more advanced than that in FIG FIG. 4B shown condition.
- the housing 102 is shown partially transparent to reveal the interior.
- FIGS. 3A and 4A show a first position in which the projections 240 of the first drum 108 engage in the slots 204 and thus a rotation of the first drum 108 is locked about the longitudinal axis 104.
- the actuator 139 now presses indirectly or directly on the second drum 106, the second drum 106 in turn pushes the bevels 242 of the first drum 108 against the action of the spring 114 by means of the bevels 220 of the contour 216, the spring 114 being compressed.
- the first drum 108 moves in a direction away from the pivot point 140 as indicated by the corresponding arrows in FIGS FIGS. 4A and 4B indicated. This movement is continued until the protrusions 240 disengage from the protrusions 200.
- the spring 114 again pushes the first drum 108 in the direction of the pivot point 140 by means of the third drum 110.
- the second drum 106 together with its bevels 220 is likewise moved again in the direction of the pivot point 140 whereby the chamfers 242 of the first drum 108 come to rest against the chamfers 206 of the housing 102 and slide therealong into a third position, as in Figs Figures 4D and 4E shown.
- the projections 240 of the first drum 108 are again located in the slots 204 of the housing 102, so that further rotation of the first drum 108 about the longitudinal axis 104 is again locked.
- the process described above may be repeated as many times as desired to rotate the first drum 108 in a defined manner relative to the other drums 106 and 110.
- the actuator 139 may be electrically, mechanically and / or pressure-operated.
- a piezoelectrically, electrostatically, semi-mechanically manually or electromagnetically operated actuator 139 is suitable.
- “Operated” here means the active principle which the actuator 139 exploits in order to generate the actuating force for actuating the first drum 106 (or, depending on the embodiment, also one of the other drums 108, 110).
- the actuator 139 may include an electromagnet that cooperates with a metal part disposed in one of the drums 106, 108, 110 that the electromagnet attracts or repels by appropriately driving it to thereby adjust the above-described adjustment of the drums 106, 108, 110 to each other to reach.
- the pressure force applied to the second drum 106 by means of the actuator 139 is typically 0.5-100 N.
- the compressive force to be applied by the actuator is reduced in accordance with the centrifugal force acting.
- a suitable, not shown, control device which controls the actuator 139, so that the drums 106, 108, 110 at the desired time to take the respective desired position to each other.
- the control device may have a timer and / or an integrated circuit.
- FIG. 5 shows again in a section, but schematically the cartridge 100 from FIG. 1 ,
- Actuator 139 is preferably formed as a piezoelectric element, which is arranged between the lid 118, which faces the pivot point 140, and the second drum 106.
- the actuator 139 is on the one hand attached to the lid 139 and pressed on the other hand with its end face 500 against the surface of the drum 106 without being connected to this.
- the face 500 is shown spaced apart from the drum 106 for clarity only. In fact, however, the return means 114 constantly urges the drum 106 against the actuator 139.
- the cartridge 100 is rotated around the fulcrum 140 at a constant speed.
- the speed is chosen such that there is no automatic rotation of the first drum 108 with respect to the other drums 106, 110, ie the ballpoint pen mechanism is not already triggered due to the centrifugal force 142.
- the actuator 139 is controlled by a corresponding control signal from the controller for applying a force to the second drum 106.
- the first drum 108 is rotated in such a way that the chambers 120, 124 are opposite one another and conductively connected to one another.
- the chambers 120, 124 closing cover (not shown) by means of mandrels 230 in this process pierced.
- a first component 502 flows from the chamber 120 into the mixing chamber 124 under the action of centrifugal force 142.
- the ball-point pen mechanism is actuated again by means of the actuator 139, whereupon the first drum 108 rotates such that the mixing chamber 124 faces the chamber 122 and with this is conductively connected.
- a cover film closing the chamber 122 as described, which is pierced in this process, may also be provided.
- a second component 504 flows from the chamber 122 into the mixing chamber 124 under the action of the centrifugal force 142 and can be mixed there.
- the movements of the respective components 139, 106, 108, 110 is indicated by arrows.
- the rotation of the first drum 108 and thus the processing of the components 502, 504 can thus take place largely independently of the centrifugal force 142.
- FIG. 6 shows a variant opposite FIG. 5 .
- an actuator 600 is provided which is fixed on the end face 500 of the actuator on the one hand and on the third drum 110 on the other hand.
- the actuator 600 extends through a hole 602 in the second drum 106 and is connected to the first drum 108 and / or the third drum 110.
- the restoring force is generated by the actuator 600, so that the spring 114 omitted, see FIG. 5 , can.
- contraction of the actuator 139 the actuator 600 and thus the drum 108 is pulled upwards.
- the rotation of the first drum 108 is performed as in the embodiment Fig. 5 ,
- FIG. 7 shows another variant opposite FIG. 5 .
- the actuator 139 is disposed between the fulcrum 140 and the second drum 106 and the first drum 108, respectively, in the embodiment according to FIG Fig. 7 the actuator 139 is disposed between the end 112 of the housing 102 and the third drum 110.
- the actuation and return of the ballpoint pen mechanism is carried out in this embodiment by means of the actuator 139 or alternatively in conjunction with the centrifugal force 142nd
- a spring 800 may additionally be provided between the lid 118 of the second drum 106, which biases the drums 106, 108, 110 against the actuator 139. This is in Fig. 8 shown, which is a variant opposite FIG. 7 represents.
- Fig. 9 shows a still further variant opposite Fig. 5 .
- FIG. 9 only the first drum 108 and the second drum 106 are provided.
- An actuator 600 in the form of a shaft is connected on the one hand to the actuator 139 and on the other hand to the first drum 108.
- the shaft 600 passes through a hole in the second drum 106, which provided fixed according to this embodiment.
- the actuator 139 in particular an electric motor rotates the shaft 600 and thereby the first drum 108 about the central axis 104, whereby different chambers 120, 122, 124 are conductively connected to each other, as described above.
- a ballpoint pen mechanism is not provided in this embodiment.
- the actuator 139 may be further configured to also move the shaft 139 along the centerline 104 to thereby space the first drum 108 from the second drum 106 for rotation and to press the drums 106, 108 together again after rotation. whereby a tight, conductive connection is provided, for example, between the chamber 120 and the chamber 124.
- the second drum 106 may be movably provided according to another embodiment by means of another actuator, not shown.
- Fig. 10 shows a still further variant opposite Fig. 5 .
- the lid 118 is designed in the form of an adapter for holding the actuator 139.
- the actuator 139 extends through the adapter 118 and thus acts directly on the second drum 106 to this in a direction away from the pivot point 140, ie in Fig. 11 down, to move.
- the actuator 139 for this purpose, an actuator, in particular a rod having, which presses against the drum 106.
- the provision can be made as described above by means of the return means 114.
- the actuator 139 such as the actuator, is fixedly connected to the second drum 106. This would allow the drum 106 to be rapidly reciprocated along the longitudinal direction 104 by means of the actuator 139, whereby a mixing chamber for mixing components in one of the chambers 120, 122 could be provided. If the amplitude of the to-and-fro motion is chosen to be sufficiently small, this movement can occur without twisting the drums 106, 108, 110 relative to each other, i. without triggering the ballpoint pen mechanism.
- the embodiment according to FIG. 11 differs from the according to FIG. 10 in that the actuator 139 is attached to the outside of the adapter 118, ie, the actuator 139 does not penetrate the adapter 118 in this case. Rather, the actuator 139 acts indirectly, for example by means of a flexible membrane, on the second drum 106 to operate them in the first direction 207.
- a section 1100 of the adapter 118 is made thin, wherein an actuated by the actuator actuator 139 600 deforms this thin portion 100 elastically.
- the actuator 139 pressure-operated, for which the actuator is pressure-conductively connected to a pressure device, not shown, for example, a gas pressure cylinder, and is driven by this.
- a pressure device not shown, for example, a gas pressure cylinder
- the adapter 118 and the second drum 106 form a chamber with each other, which is acted upon by pressure from the pressure device and thus the actuator 139 is formed.
- the actuator 139 could be provided in the form of a bellows, which is provided between the adapter 118 and the second drum 106.
- the actuator 139 can also be provided elsewhere, for example between the first drum 108 and the second or third drum 106, 110.
- the actuator 139 is preferably designed as a piezoelectric element.
- Fig. 12 shows in a plan view schematically a centrifuge 1200 according to an embodiment of the present invention.
- the centrifuge 1200 includes a rotor, not shown.
- the cartridge 100 is inserted into the rotor and is moved on a circular path 1202.
- a coil 1204 is provided, which drives the actuator 139 as soon as it passes through the coil 1204.
- the actuator 139 then triggers the above-described rotational movement of the first drum 108.
- a transmitting device not shown, could be provided which generates electromagnetic waves, in particular radio waves, short range.
- An unillustrated, integrated into the cartridge 100 receiver device then controls the actuator 139, as soon as it passes the transmitter.
- the actuator 139 can therefore be controlled wirelessly by a stationary control device, for example provided in the centrifuge housing. But also a control by means of sliding contact is conceivable.
- the cartridge 100 could include a computer unit, not shown, which controls the actuator 139 on the basis of a computer program.
- a start time of the computer program can be set by a user or transmitted wirelessly to the computer device by means of the aforementioned control device.
- a power supply, not shown, for the actuator 139 can be integrated in the cartridge 100, for example in the form of a battery in the housing 102.
- Electrical energy can be supplied externally wirelessly or by wire.
- the energy can be induced by means of one or more coils (wireless).
- the energy can be supplied via a sliding contact (wired).
- a control unit controls the interaction of the actuator 139, which determines the spatial positioning of the drums 106, 108, 110, and the rotational speed of the centrifuge 1200 and the rotor, which in particular controls the flow rate of the components 502, 504 through the cartridge 100.
- the second drum 106 and / or the third drum 110 may be fixed or movable with respect to the housing 102.
- the drums 106, 110 may be provided, for example, each rotatable about the central axis 104 by means of a further actuator.
- the mixing chamber 124 may comprise an obstacle structure, not shown, for example a sieve or a grid structure which is adapted to move through the liquids 502, 504 under the action of a centrifugal force (ie when the rotational speed of the centrifuge exceeds a predetermined threshold). in order to mix these.
- an obstacle structure not shown, for example a sieve or a grid structure which is adapted to move through the liquids 502, 504 under the action of a centrifugal force (ie when the rotational speed of the centrifuge exceeds a predetermined threshold). in order to mix these.
- the housing 102 and the drums 106, 108, 110 may be made of the same or different polymers.
- the one or more polymers are, in particular, thermoplastics, elastomers or thermoplastic elastomers. Examples are cycloolefin polymer (COP), cycloolefin copolymer (COC), polycarbonates (PC), polyamides (PA), polyurethanes (PU), polypropylene (PP), polyethylene terephthalate (PET) or poly (methyl methacrylate) ( PMMA).
- COP cycloolefin polymer
- COC cycloolefin copolymer
- PC polycarbonates
- PA polyamides
- PU polyurethanes
- PP polypropylene
- PET polyethylene terephthalate
- PMMA poly (methyl methacrylate)
- the second drum 106 and / or the third drum 110 may be formed integrally with the housing 102.
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- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Centrifugal Separators (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
Abstract
Description
Die Durchführung biochemischer Prozesse basiert insbesondere auf der Handhabung von Flüssigkeiten. Typischerweise wird diese Handhabung manuell mit Hilfsmitteln wie Pipetten, Reaktionsgefäßen, aktiven Sondenoberflächen oder Laborgeräten durchgeführt. Durch Pipettierroboter oder Spezialgeräte sind diese Prozesse zum Teil bereits automatisiert.The implementation of biochemical processes is based in particular on the handling of liquids. Typically, this manipulation is done manually with tools such as pipettes, reaction vessels, active probe surfaces or laboratory equipment. By pipetting robots or special equipment, these processes are already partially automated.
Lab-on-a-Chip-Systeme (auch als Westentaschenlabor oder Chiplabor bezeichnet) bringen die gesamte Funktionalität eines makroskopischen Labors auf einem nur plastikkartengroßen Kunststoffsubstrat unter. Lab-on-a-Chip-Systeme bestehen typischerweise aus zwei Hauptkomponenten. Ein Testträger beinhaltet Strukturen und Mechanismen für die Umsetzung der fluidischen Grundoperationen (z.B. Mischer), welche aus passiven Komponenten, wie Kanälen, Reaktionskammern und vorgelagerten Reagenzien, oder auch aktiven Komponenten wie Ventilen oder Pumpen bestehen können. Die zweite Hauptkomponente sind Aktuations-, Detektions- und Steuereinheiten. Solche Systeme ermöglichen es, biochemische Prozesse vollautomatisiert durchzuführen.Lab-on-a-Chip systems (also referred to as the West Pocket Laboratory or the Chiplabor) accommodate all the functionality of a macroscopic laboratory on a plastic plastic-sized plastic substrate. Lab-on-a-chip systems typically consist of two major components. A test carrier includes structures and mechanisms for the implementation of basic fluidic operations (e.g., mixers), which may consist of passive components such as channels, reaction chambers and upstream reagents, or even active components such as valves or pumps. The second main component is actuation, detection and control units. Such systems make it possible to carry out biochemical processes fully automatically.
Ein Lab-on-a-Chip-System ist beispielsweise in der Druckschrift
Die in dem Anspruch 1 definierte Kartusche, die in dem Anspruch 13 definierte Zentrifuge sowie das in dem Anspruch 15 definierte Verfahren weisen gegenüber herkömmlichen Lösungen den Vorteil auf, dass die Prozessierung wenigstens einer Komponente in der Kartusche unabhängig von der Drehzahl der Zentrifuge erfolgen kann.The cartridge defined in claim 1, the centrifuge defined in claim 13 and the method defined in claim 15 have the advantage over conventional solutions that the processing of at least one component in the cartridge can be carried out independently of the rotational speed of the centrifuge.
D.h., bei der ersten Variante, bei welcher die erste Trommel die erste Kammer aufweist, lassen sich die erste und zweite Kammer oder die erste und dritte Kammer mittels Betätigung des Aktors miteinander leitend verbinden. Zusätzlich kann die Betätigung noch von der Zentrifugalkraft unterstützt werden.That is, in the first variant in which the first drum has the first chamber, the first and second chambers or the first and third chambers can be conductively connected to one another by actuation of the actuator. In addition, the operation can still be supported by the centrifugal force.
Beispielsweise wird bei der ersten Variante wenigstens eine erste Komponente in der zweiten Kammer und eine zweite Komponente in der dritten Kammer vorgehalten. Mittels Betätigung des Aktors wird die erste Kammer mit der zweiten Kammer leitend verbunden, und die erste Komponente wird hiernach auf Grund der Wirkung der Zentrifugalkraft aus der zweiten Kammer in die erste Kammer transferiert. Hiernach wird die erste Kammer wiederum mittels Betätigung des Aktors mit der dritten Kammer verbunden, und die zweite Komponente fliest hiernach ebenfalls in die erste Kammer, beispielweise um mit der ersten Komponente vermischt zu werden.For example, in the first variant at least one first component in the second chamber and a second component in the third chamber held. By actuation of the actuator, the first chamber is conductively connected to the second chamber, and the first component is thereafter transferred from the second chamber to the first chamber due to the action of the centrifugal force. Thereafter, the first chamber is in turn connected by actuation of the actuator with the third chamber, and the second component thereafter also flows into the first chamber, for example in order to be mixed with the first component.
Alternativ könnte bei der ersten Variante aber auch eine einzige Komponente in der ersten Kammer enthalten sein. Mittels Betätigung des Aktors wird die erste Kammer zunächst mit der zweiten Kammer leitend verbunden und ein bestimmter Anteil der Komponente in die zweite Kammer abgefüllt. Hiernach wird die erste Kammer mit der dritten Kammer leitend verbunden und ein weiterer Anteil der Komponente in die dritte Kammer abgefüllt.Alternatively, in the first variant, however, a single component could also be contained in the first chamber. By actuation of the actuator, the first chamber is first conductively connected to the second chamber and filled a certain proportion of the component in the second chamber. Thereafter, the first chamber is conductively connected to the third chamber and filled a further portion of the component in the third chamber.
D.h., bei der zweiten Variante, bei welcher die erste Trommel die erste und die zweite Kammer aufweist, lassen sich die erste und dritte Kammer oder die zweite und dritte Kammer mittels Betätigung des Aktors miteinander leitend verbinden. Zusätzlich kann die Betätigung noch von der Zentrifugalkraft unterstützt werden.That is, in the second variant, in which the first drum has the first and the second chamber, the first and third chambers or the second and third chambers can be conductively connected to one another by actuation of the actuator. In addition, the operation can still be supported by the centrifugal force.
Beispielsweise wird bei der zweiten Variante wenigstens eine erste Komponente in der ersten Kammer und eine zweite Komponente in der zweiten Kammer vorgehalten. Mittels Betätigung des Aktors wird die erste Kammer mit der dritten Kammer leitend verbunden, und die erste Komponente wird hiernach auf Grund der Wirkung der Zentrifugalkraft aus der ersten Kammer in die dritte Kammer transferiert. Hiernach wird die zweite Kammer wiederum mittels Betätigung des Aktors mit der dritten Kammer verbunden, und die zweite Komponente fliest hiernach in ebenfalls in die dritte Kammer, beispielweise um mit der ersten Komponente vermischt zu werden.For example, in the second variant at least one first component in the first chamber and a second component in the second chamber held. By actuation of the actuator, the first chamber is conductively connected to the third chamber, and the first component is thereafter transferred from the first chamber to the third chamber due to the action of the centrifugal force. Thereafter, the second chamber is in turn connected by actuation of the actuator with the third chamber, and the second component thereafter flows into the third chamber, for example, to be mixed with the first component.
Alternativ könnte bei der zweiten Variante aber auch eine einzige Komponente in der dritten Kammer enthalten sein. Mittels Betätigung des Aktors wird die erste Kammer zunächst mit der dritten Kammer leitend verbunden und ein bestimmter Anteil der Komponente in die erste Kammer abgefüllt. Hiernach wird die zweite Kammer mit der dritten Kammer leitend verbunden und ein weiterer Anteil der Komponente in die zweite Kammer abgefüllt.Alternatively, in the second variant, however, a single component could also be contained in the third chamber. By actuating the actuator, the first chamber is first conductively connected to the third chamber and filled a certain proportion of the component in the first chamber. Thereafter, the second chamber is conductively connected to the third chamber and filled a further portion of the component in the second chamber.
Die erste und zweite Variante lassen sich auch kombinieren.The first and second variants can also be combined.
Aus den Unteransprüchen ergeben sich vorteilhafte Ausgestaltungen der Erfindung.From the dependent claims, advantageous embodiments of the invention.
"Komponente" meint vorliegend eine Flüssigkeit, ein Gas oder einen Partikel."Component" in this case means a liquid, a gas or a particle.
Mit "Kammer" ist vorliegend bevorzugt ein Leitungsabschnitt, welcher beidseitig oder lediglich einseitig offen ausgebildet ist, sowie ein im Wesentlichen geschlossener Raum gemeint, welcher über einen Zu- und/oder Ablauf verfügt.By "chamber" is presently preferably a line section, which is formed on both sides or only open on one side, as well as a substantially closed space meant, which has an inlet and / or outlet.
"wahlweise" ist vorliegend dahingehend zu verstehen, dass die erste Kammer entweder mit der zweiten oder mit der dritten Kammer (erste Variante) bzw. entweder die erste Kammer mit der dritten Kammer oder die zweite Kammer mit der dritten Kammer (zweite Variante) in Abhängigkeit von einer Betätigung durch den Aktor verbunden wird."optionally" is to be understood in the present case that the first chamber with either the second or with the third chamber (first variant) or either the first chamber with the third chamber or the second chamber with the third chamber (second variant) in dependence is connected by an actuation by the actuator.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche ist der Aktor elektrisch-, mechanisch- und/oder druckbetriebenen vorgesehen. Insbesondere bietet sich ein piezoelektrisch-, elektrostatisch-, semi-mechanisch-manuell oder elektromagnetischbetriebener Aktor an. Druckbetrieben meint, dass der Aktor die erste Trommel unter Ausnutzung eines Gas- oder Flüssigkeitsdrucks betätigt.According to one embodiment of the cartridge according to the invention, the actuator is provided electrically, mechanically and / or pressure-operated. In particular, a piezoelectrically, electrostatically, semi-mechanically manually or electromagnetically operated actuator is suitable. Printing companies means that the actuator operates the first drum by utilizing a gas or liquid pressure.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche weist der Aktor ein Stellglied auf, welches mit der ersten Trommel verbunden ist, um diese direkt zu drehen und ggf. entlang der Mittelachse zu bewegen. Dadurch ergibt sich eine zuverlässige direkte Betätigung der ersten Trommel, wobei auf die Verwendung der nachstehend beschriebenen "Kugelschreibermechanik" verzichtet wird. "direkt" meint hier im Unterschied zu "indirekt", dass keine weiteren mechanischen Elemente zur Umsetzung der Bewegung zwischengeschaltet werden.According to one embodiment of the cartridge according to the invention, the actuator has an actuator which is connected to the first drum in order to rotate it directly and if necessary to move along the central axis. This results in a reliable direct operation of the first drum, wherein the use of the "ballpoint pen mechanism" described below is dispensed with. "direct" here, unlike "indirectly", means that no further mechanical elements are interposed to implement the movement.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche weist der Aktor ein Stellglied auf, welches mit der ersten Trommel verbunden ist, um diese entlang der Mittelachse zu bewegen und dadurch zu drehen. Bevorzugt betätigt das Stellglied die nachstehend beschriebene Kugelschreibermechanik, woraufhin die Kugelschreibermechanik die erste Trommel dreht. Die erste Trommel wird somit indirekt betätigt.According to one embodiment of the cartridge according to the invention, the actuator has an actuator, which is connected to the first drum to move along the central axis and thereby rotate. Preferably, the actuator actuates the ballpoint pen mechanism described below, whereupon the ballpoint pen mechanism rotates the first drum. The first drum is thus indirectly operated.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche umfasst die Verstelleinrichtung eine erste Schräge, welche mit einer zweiten Schräge der ersten Trommel zusammenwirkt, um diese aus einer ersten Stellung, in der diese mit einem Gehäuse der Kartusche in Drehrichtung um die Mittelachse formschlüssig in Eingriff steht, in eine zweite Stellung entlang der Mittelachse zu verbringen, in welcher der Formschluss aufgehoben ist und sich die erste Trommel um die Mittelachse auf Grund der Wirkung eines Rückstellmittels oder des Aktors oder eines weiteren Aktors dreht. Dadurch wird eine Kugelschreibermechanik vorgesehen.According to one embodiment of the cartridge according to the invention, the adjusting device comprises a first bevel, which cooperates with a second bevel of the first drum, in order from a first position in which this is a form-locking engagement with a housing of the cartridge in the rotational direction about the central axis in a second position along the central axis to spend, in which the positive connection is canceled and the first drum rotates about the central axis due to the action of a return means or the actuator or another actuator. As a result, a ballpoint pen mechanism is provided.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche betätigt der Aktor die erste Schräge für das Zusammenwirken mit der zweiten Schräge. Bevorzugt bewegt der Aktor die erste Schräge in einer Richtung weg von dem Drehpunkt der Kartusche der Zentrifuge entlang der Mittelachse.According to one embodiment of the cartridge according to the invention, the actuator actuates the first slope for cooperation with the second slope. Preferably, the actuator moves the first slope in a direction away from the pivot point of the cartridge of the centrifuge along the central axis.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche ist der ersten Trommel eine zweite Trommel bezogen auf die Mittelachse vor- oder nachgelagert, wobei der Aktor die zweite Trommel für das Drehen der ersten Trommel betätigt. Die erste Trommel wird somit indirekt mittels der zweiten Trommel betätigt.According to one embodiment of the cartridge according to the invention the first drum is a second drum upstream or downstream relative to the central axis, wherein the actuator actuates the second drum for rotating the first drum. The first drum is thus actuated indirectly by means of the second drum.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche liegt der Aktor gegen die erste und/oder zweite Trommel für ein Drücken gegen diese flächig an, oder der Aktor ist mit der ersten oder zweiten Trommel fest verbunden. Die Ausgestaltung, wonach der Aktor gegen die erste und/oder zweite Trommel für ein Drücken gegen diese flächig anliegt, zeichnet sich durch einen einfachen Aufbau und eine einfache Montage aus. Die feste Verbindung zwischen der ersten oder zweiten Trommel und dem Aktor hat den Vorteil, dass der Aktor die erste oder zweite Trommel in entgegengesetzte Richtungen bewegen kann.According to one embodiment of the cartridge according to the invention, the actuator abuts flat against the first and / or second drum for pressing against it, or the actuator is fixedly connected to the first or second drum. The configuration according to which the actuator abuts flat against the first and / or second drum for pressing against it, is characterized by a simple construction and a simple assembly. The fixed connection between the first or second drum and the actuator has the advantage that the actuator can move the first or second drum in opposite directions.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche ist der Aktor zwischen der ersten und zweiten Trommel angeordnet. Dazu ist der Aktor vorzugsweise als piezoelektrisches Element ausgebildet.According to one embodiment of the cartridge according to the invention, the actuator is arranged between the first and second drum. For this purpose, the actuator is preferably designed as a piezoelectric element.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche weist die Kartusche ein Gehäuse auf, welches an seinem einen Ende mittels eines Adapters verschlossen ist, wobei der Aktor an dem Adapter befestigt ist. Dadurch werden mehrere Funktionen in den Adapter integriert: nämlich einmal ein insbesondere steriler Verschluss des Gehäuses und weiterhin die Aufnahme des Aktors. Bevorzugt ist der Aktor in den Adapter integriert.According to one embodiment of the cartridge according to the invention, the cartridge has a housing which is closed at one end by means of an adapter, wherein the actuator is attached to the adapter. As a result, several functions are integrated into the adapter: namely, once a particular sterile closure of the housing and continue to accommodate the actuator. Preferably, the actuator is integrated in the adapter.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche weist der Adapter eine flexible Membran auf, welche an ihrer einen Seite mittels des Aktors betätigbar ist und an ihrer anderen Seite auf die erste Trommel oder die zweite Trommel wirkt. Dadurch kann ein sterilerer Abschluss geschaffen werden. Der Aktor liegt somit bevorzugt außerhalb des Innenraums des Gehäuses.According to one embodiment of the cartridge according to the invention, the adapter has a flexible membrane which can be actuated on its one side by means of the actuator and acts on its other side on the first drum or the second drum. This can create a more sterile outcome. The actuator is thus preferably outside the interior of the housing.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche ist die zweite und/oder dritte Kammer der ersten Trommel bezogen auf die Mittelachse vor- oder nachgelagert und/oder in der zweiten Trommel ausgebildet ist. Beispielsweise kann auch eine zweite Kammer der ersten Trommel vor- und eine dritte Kammer der ersten Trommel nachgelagert sein. Insbesondere ist die zweite Kammer in der zweiten Trommel und die dritte Kammer in einer dritten Trommel vorgesehen.According to one embodiment of the cartridge according to the invention, the second and / or third chamber of the first drum upstream or downstream relative to the central axis and / or is formed in the second drum. For example, a second chamber of the first drum and a third chamber of the first drum may be downstream. In particular, the second chamber is provided in the second drum and the third chamber is provided in a third drum.
Gemäß einer Ausgestaltung der erfindungsgemäßen Kartusche ist der Aktor vor oder hinter der ersten Trommel in Bezug auf einen Drehpunkt der Kartusche in der Zentrifuge angeordnet. Wird der Aktor hinter der ersten Trommel in Bezug auf den Drehpunkt angeordnet, ergibt sich vorteilhaft, dass kein sonstiges Rückstellmittel erforderlich ist bzw. dieses von dem Aktor selbst ausgebildet wird. Die Betätigung der ersten Trommel erfolgt dabei durch den Aktor in Zusammenspiel mit der Zentrifugalkraft.According to one embodiment of the cartridge according to the invention, the actuator is arranged in front of or behind the first drum with respect to a pivot point of the cartridge in the centrifuge. If the actuator is arranged behind the first drum with respect to the fulcrum, it is advantageous that no other return means is required or this is formed by the actuator itself. The actuation of the first drum is effected by the actuator in cooperation with the centrifugal force.
Ausführungsbeispiele der Erfindung sind in den Figuren der Zeichnung dargestellt und in der nachfolgenden Beschreibung näher erläutert.Embodiments of the invention are illustrated in the figures of the drawing and explained in more detail in the following description.
Es zeigen:
- Figur 1
- schematisch einen Schnitt durch eine Kartusche gemäß einem Ausführungsbeispiel der vorliegenden Erfindung;
- Figuren 2A-2G
- perspektivische Ansichten verschiedener Bauteile der Kartusche aus
Figur 1 ; - Figuren 3A-3E
- verschiedene Betriebszustände der Kartusche aus
Figur 1 ; - Figuren 4A-4E
- Detailansichten einer Verstelleinrichtung entsprechend den verschiedenen Betriebszuständen aus
Figur 3A-3E ; - Figur 5
- in einem Schnitt nochmals, aber schematisch die Kartusche aus
Figur 1 ; - Figur 6
- eine Variante gegenüber
Figur 5 , wobei der Aktor eine Welle umfasst; - Figur 7
- eine Variante gegenüber
Figur 5 ohne Feder; - Figur 8
- eine weitere Variante gegenüber
Figur 5 , wobei die Anordnung von Aktor und Feder vertauscht ist; - Figur 9
- eine weitere Variante gegenüber
Figur 5 , wobei der Aktor eine Welle umfasst und keine Kugelschreibermechanik vorgesehen ist; - Figur 10
- eine weitere Variante gegenüber
Figur 5 , wobei der Deckel des Gehäuses als Adapter ausgebildet ist; - Figur 11
- eine weitere Variante gegenüber
Figur 10 , wobei der Adapter mit einer Membran ausgebildet ist; und - Figur 12
- schematisch eine Zentrifuge gemäß einem Ausführungsbeispiel der vorliegenden Erfindung.
- FIG. 1
- schematically a section through a cartridge according to an embodiment of the present invention;
- Figures 2A-2G
- perspective views of various components of the cartridge
FIG. 1 ; - Figures 3A-3E
- different operating conditions of the cartridge
FIG. 1 ; - Figures 4A-4E
- Detail views of an adjustment according to the different operating conditions
Figure 3A-3E ; - FIG. 5
- in a section again, but schematically the cartridge off
FIG. 1 ; - FIG. 6
- a variant opposite
FIG. 5 wherein the actuator comprises a shaft; - FIG. 7
- a variant opposite
FIG. 5 without feather; - FIG. 8
- another variant opposite
FIG. 5 wherein the arrangement of actuator and spring is reversed; - FIG. 9
- another variant opposite
FIG. 5 wherein the actuator comprises a shaft and no ballpoint pen mechanism is provided; - FIG. 10
- another variant opposite
FIG. 5 wherein the cover of the housing is designed as an adapter; - FIG. 11
- another variant opposite
FIG. 10 wherein the adapter is formed with a membrane; and - FIG. 12
- schematically a centrifuge according to an embodiment of the present invention.
In den Figuren bezeichnen gleiche Bezugszeichen gleiche oder funktionsgleiche Elemente, soweit nichts Gegenteiliges angegeben ist.In the figures, like reference numerals designate like or functionally identical elements, unless indicated otherwise.
Die Kartusche 100 umfasst ein Gehäuse 102 in Form eines Röhrchens. Beispielsweise kann das Gehäuse 102 als ein 15 mL Zentrifugenröhrchen, 1.5 mL oder 2 mL Eppendorfröhrchen oder alternativ als eine Mikrotiterplatte (z.B. 20 µL pro Kavität) ausgebildet sein. Die Längsachse des Gehäuses 102 ist mit 104 bezeichnet.The
In dem Gehäuse 102 sind beispielsweise eine erste Trommel 108, eine zweite Trommel 106 und eine dritte Trommel 110 aufgenommen. Die Trommeln 106, 108, 110 sind hintereinander und mit ihren jeweiligen Mittelachsen koaxial mit der Längsachse 104 angeordnet.In the
Das Gehäuse 102 ist an seinem einen Ende 112 geschlossen ausgebildet. Zwischen dem geschlossenen Ende 112 und der zu diesem benachbart angeordneten Trommel 110 ist ein Rückstellmittel beispielsweise in Form einer Feder 114 angeordnet. Die Feder 114 kann in Form einer Spiralfeder oder eines Polymers, insb. eines Elastomers, ausgebildet sein. Das andere Ende 116 des Gehäuses 102 ist mittels eines Verschlusses 118 verschlossen. Bevorzugt kann der Verschluss 118 abgenommen werden, um die Trommeln 106, 108, 110 aus dem Gehäuse 102 zu entnehmen. Alternativ kann auch das Gehäuse 102 selbst zerlegbar sein, um die Trommeln 106, 108, 110 zu entnehmen oder an die Kammern, beispielsweise die Kammer 136, zu gelangen.The
Gemäß einem weiteren Ausführungsbeispiel ist die Feder 114 zwischen dem Verschluss 118 und der Trommel 106 angeordnet, sodass die Feder 114 zum Erzeugen einer Rückstellkraft gedehnt wird. Auch andere Anordnungen der Feder 114 sind denkbar.According to another embodiment, the
Eine jeweilige Trommel 106, 108, 110 kann eine oder mehrere Kammern aufweisen:A
So umfasst beispielsweise die zweite Trommel 106 mehrere Kammern 120 für Reagenzien sowie eine weitere Kammer 122 zur Aufnahme einer Probe, beispielsweise einer Blutprobe, die von einem Patienten entnommen wurde.For example, the
Die der zweiten Trommel 106 nachgeschaltete erste Trommel 108 umfasst eine Mischkammer 124, in welcher die Reagenzien aus den Kammern 120 mit der Probe aus der Kammer 122 gemischt werden. Außerdem umfasst die erste Trommel 108 beispielsweise eine Kammer 126, in welcher das Gemisch aus der Mischkammer 124 in eine flüssige und eine feste Phase 128 bzw. 130 getrennt wird. Bei der festen Phase 130 kann es sich um eine Gelsäule, eine Silicamatrix oder einen Filter handeln.The
Die der ersten Trommel 108 wiederum nachgeschaltete dritte Trommel 110 umfasst eine Kammer 132 zur Aufnahme eines Abfallprodukts 134 aus der Kammer 126. Weiterhin umfasst die dritte Trommel 110 eine weitere Kammer 136 zur Aufnahme des gewünschten Endprodukts 138.The
Die Kartusche 100 weist eine äußere Geometrie auf, so dass diese in eine Aufnahme eines Rotors einer Zentrifuge, insbesondere in eine Aufnahme eines Ausschwingrotors oder Festwinkelrotors einer Zentrifuge, eingesetzt werden kann. Während des Zentrifugierens wird die Kartusche 100 um einen in
Ziel ist es nun, verschiedene Prozesse innerhalb der Kartusche 100 mittels eines Aktors 139 zu steuern. So soll beispielsweise die Mischkammer 124 zunächst mit der Kammer 122 fluidisch verbunden werden, um die Probe aus der Kammer 122 aufzunehmen. Hiernach soll die Mischkammer 124 mit den Kammern 120 verbunden werden, um die Reagenzien aus diesen aufzunehmen. Anschließend sollen die Reagenzien und die Probe in der Mischkammer 124 gemischt werden. Ähnlich sollen auch die Prozesse in den Kammern 126, 132 und 136 gesteuert werden. Zusätzlich zur Steuerung der Prozesse mittels des Aktors 139 kann auch die Drehzahl der Zentrifuge bzw. eines Rotors derselben entsprechend gesteuert werden. Die wirkende Zentrifugalkraft 142 hat insbesondere die Aufgabe, die Komponenten, also beispielsweise die Probe oder die Reagenzien, zwischen den Kammern 120, 122 und der Mischkammer 124 zu transferieren.The aim is now to control various processes within the
Die
Wie in
Die
Der Aktor 139, die Vorsprünge 200, die Schlitze 204, die Schrägen 206, die Vorsprünge 212, die Schrägen 218, 220, die Vorsprünge 240 sowie die Schrägen 242 bilden im Zusammenspiel mit der Rückstellfeder 114 die vorstehend erwähnte Verstelleinrichtung 300 zum definierten Verdrehen der ersten Trommel 108 gegenüber den anderen Trommeln 106, 110 um die Längsachse 104.The
Die
Gibt der Aktor 139 nunmehr die zweite Trommel 106 frei, so drückt die Feder 114 die erste Trommel 108 mittels der dritten Trommel 110 wieder in Richtung des Drehpunkts 140. Dadurch wird die zweite Trommel 106 samt ihrer Schrägen 220 ebenfalls wieder in Richtung des Drehpunkts 140 bewegt, wodurch die Schrägen 242 der ersten Trommel 108 gegen die Schrägen 206 des Gehäuses 102 zum Liegen kommen und entlang dieser unter Ausführung einer weiteren Drehbewegung der ersten Trommel 108 in eine dritte Stellung gleiten, wie in den
Der vorstehend beschriebene Prozess kann beliebig oft wiederholt werden, um die erste Trommel 108 definiert gegenüber den anderen Trommeln 106 und 110 zu drehen.The process described above may be repeated as many times as desired to rotate the
Weiter alternativ könnte anstelle des Rückstellmittels 114 auch ein weiterer nicht dargestellter Aktor verwendet werden.Further alternatively, instead of the return means 114, another actuator, not shown, could be used.
Grundsätzlich kann der Aktor 139 elektrisch-, mechanisch- und/oder druckbetrieben sein. Insbesondere bietet sich ein piezoelektrisch-, elektrostatisch-, semi-mechanisch-manuell oder elektromagnetischbetriebener Aktor 139 an. "betrieben" meint hier das Wirkprinzip, welches der Aktor 139 ausnutzt, um die Betätigungskraft zum Betätigen der ersten Trommel 106 (oder je nach Ausführungsform auch eine der anderen Trommeln 108, 110) zu erzeugen. Beispielweise kann der Aktor 139 einen Elektromagneten aufweisen, welcher mit einem in einer der Trommeln 106, 108, 110 angeordneten Metallteil zusammenwirkt, das der Elektromagnet mittels geeigneter Ansteuerung desselben anzieht oder abstößt, um dadurch die oben erläuterte Verstellung der Trommeln 106, 108, 110 zueinander zu erreichen. Die auf die zweite Trommel 106 mittels des Aktors 139 aufgebrachte Druckkraft beträgt typischerweise 0,5-100 N. Die von dem Aktor aufzubringende Druckkraft reduziert sich entsprechend der wirkenden Zentrifugalkraft.In principle, the
Bevorzugt ist eine geeignete, nicht dargestellte Steuereinrichtung vorgesehen, welche den Aktor 139 ansteuert, sodass die Trommeln 106, 108, 110 zu der gewünschten Zeit die jeweils gewünschte Position zueinander einnehmen. Dazu kann die Steuereinrichtung einen Timer und/oder einen integrierten Schaltkreis aufweisen.Preferably, a suitable, not shown, control device is provided, which controls the
Bei dem Ausführungsbeispiel nach den
Beispielsweise wird die Kartusche 100 mit einer konstanten Drehzahl um den Drehpunkt 140 gedreht. Die Drehzahl ist dabei derart gewählt, dass es zu keinem selbsttätigen Verdrehen der ersten Trommel 108 gegenüber den anderen Trommeln 106, 110 kommt, d.h. die Kugelschreibermechanik nicht schon auf Grund der Zentrifugalkraft 142 ausgelöst wird.For example, the
Sodann wird der Aktor 139 mittels eines entsprechenden Steuersignals von der Steuereinrichtung zum Ausüben einer Kraft auf die zweite Trommel 106 gesteuert. Auf Grund des vorstehend beschriebenen Zusammenspiels zwischen den Schrägen 206, 220, 242 sowie des Rückstellmittels 114 ("Kugelschreibermechanik") kommt es zu dem Verdrehen der ersten Trommel 108 derart, dass sich die Kammern 120, 124 gegenüberliegen und miteinander leitend verbunden sind. Beispielweise wird eine ggf. vorgesehene, die Kammern 120, 124 verschließende Abdeckfolie (nicht dargestellt) mittels Dornen 230 in diesem Prozess durchstoßen. Dadurch fließt eine erste Komponente 502 unter Einwirkung der Zentrifugalkraft 142 aus der Kammer 120 in die Mischkammer 124. Hiernach wird die Kugelschreibermechanik erneut mittels des Aktors 139 betätigt, woraufhin sich die erste Trommel 108 derart dreht, dass die Mischkammer 124 der Kammer 122 gegenüberliegt und mit dieser leitend verbunden ist. Eine die Kammer 122 verschließende Abdeckfolie, wie verstehend beschrieben, welche in diesem Prozess durchstoßen wird, kann ebenfalls vorgesehen sein. Dadurch fließt eine zweite Komponente 504 unter Einwirkung der Zentrifugalkraft 142 aus der Kammer 122 in die Mischkammer 124 und kann dort vermischt werden. Die Bewegungen der jeweiligen Komponenten 139, 106, 108, 110 ist mit Pfeilen angedeutet.Then, the
Das Drehen der ersten Trommel 108 und damit die Prozessierung der Komponenten 502, 504 kann somit weitgehend unabhängig von der Zentrifugalkraft 142 erfolgen.The rotation of the
Gegenüber dem Ausführungsbeispiel nach
Während bei dem Ausführungsbeispiel nach
Reicht die vorhandene Zentrifugalkraft 142 nicht aus (beispielsweise weil die Zentrifugalkraft 142 konstant gehalten werden soll) kann zusätzlich eine Feder 800 zwischen dem Deckel 118 der zweiten Trommel 106 vorgesehen sein, welche die Trommeln 106, 108, 110 gegen den Aktor 139 vorspannt. Dies ist in
Bei dem Ausführungsbeispiel gemäß
Die zweite Trommel 106 kann gemäß einem anderen Ausführungsbeispiel mittels eines weiteren, nicht dargestellten Aktors beweglich vorgesehen sein.The
Bei dem Ausführungsbeispiel gemäß
Alternativ ist der Aktor 139, beispielsweise das Stellglied, fest mit der zweiten Trommel 106 verbunden. Dadurch ließe sich die Trommel 106 mittels des Aktors 139 schnell hin und her entlang der Längsrichtung 104 bewegen, wodurch eine Mischkammer zum Mischen von Komponenten in einer der Kammern 120, 122 vorgesehen werden könnte. Wird die Amplitude der Hin- und Herbewegung ausreichend klein gewählt, kann diese Bewegung ohne Verdrehen der Trommeln 106, 108, 110 zueinander geschehen, d.h. ohne Auslösen der Kugelschreibermechanik.Alternatively, the
Das Ausführungsbeispiel gemäß
Weiterhin besteht die Möglichkeit, den Aktor 139 druckbetrieben vorzusehen, wozu der Aktor druckleitend mit einer nicht dargestellten Druckeinrichtung, beispielsweise einem Gasdruckzylinder, verbunden ist und von dieser angetrieben wird. Im einfachsten Fall bilden der Adapter 118 und die zweite Trommel 106 eine Kammer miteinander, welche mit Druck von der Druckeinrichtung beaufschlagt und somit der Aktor 139 gebildet wird. Weiterhin könnte der Aktor 139 in Form eines Balgs vorgesehen werden, welcher zwischen dem Adapter 118 und der zweiten Trommel 106 vorgesehen ist.Furthermore, it is possible to provide the
Der Aktor 139 kann auch an anderer Stelle vorgesehen sein, beispielsweise zwischen der ersten Trommel 108 und der zweiten oder dritten Trommel 106, 110. Dazu ist der Aktor 139 bevorzugt als piezoelektrisches Element ausgebildet.The
Die Zentrifuge 1200 umfasst einen nicht weiter dargestellten Rotor. In den Rotor ist die Kartusche 100 eingesetzt und wird auf einer Kreisbahn 1202 bewegt. Entlang der Kreisbahn 1202 ist eine Spule 1204 vorgesehen, welche den Aktor 139 ansteuert, sobald dieser die Spule 1204 passiert. Der Aktor 139 löst dann die vorstehend beschriebene Drehbewegung der ersten Trommel 108 aus.The
Anstelle der Spule 1204 könnte eine nicht dargestellte Sendeinrichtung vorgesehen sein, welche elektromagnetischen Wellen, insbesondere Radiowellen, geringer Reichweite erzeugt. Eine nicht dargestellte, in die Kartusche 100 integrierte Empfängereinrichtung steuert dann den Aktor 139 an, sobald dieser die Sendeeinrichtung passiert.Instead of the
Grundsätzlich kann der Aktor 139 also drahtlos von einer stationären, beispielweise im Zentrifugengehäuse vorgesehenen Steuereinrichtung angesteuert werden. Aber auch eine Ansteuerung mittels Schleifkontakt ist denkbar.In principle, the
Alternativ könnte die Kartusche 100 eine nicht dargestellte Rechnereinheit aufweisen, welche den Aktor 139 auf Basis eines Computerprogramms ansteuert. Beispielweise kann ein Startzeitpunkt des Computerprogramms von einem Benutzer festgelegt oder drahtlos mittels der vorstehend erwähnten Steuereinrichtung auf die Rechnereinrichtung übertragen werden.Alternatively, the
Eine nicht dargestellte Energieversorgung für den Aktor 139 kann in die Kartusche 100 integriert sein, beispielweise in Form einer Batterie in dem Gehäuse 102. Elektrische Energie kann extern drahtlos oder drahtgebunden zugeführt werden. Beispielsweise kann die Energie mittels einer oder mehrerer Spulen induziert werden (drahtlos). Oder die Energie kann über einen Schleifkontakt zugeführt werden (drahtgebunden).A power supply, not shown, for the
Eine nicht dargestellte Steuereinheit regelt das Zusammenspiel des Aktors 139, welcher die räumliche Positionierung der Trommeln 106, 108, 110 vorgibt, und der Drehzahl der Zentrifuge 1200 bzw. des Rotors, welche insbesondere die Fließgeschwindigkeit der Komponenten 502, 504 durch die Kartusche 100 steuert.A control unit, not shown, controls the interaction of the
Vorteilhaft können bei den vorstehend beschriebenen Ausführungsbeispielen unabhängig von der Drehzahl des Rotors der Zentrifuge 1200 unterschiedliche Kammern miteinander verschaltet, d.h. miteinander leitend verbunden werden. Dieses Verschalten kann beispielsweise bei konstanter, steigender oder fallender Drehzahl erfolgen.Advantageously, in the embodiments described above, independent of the rotational speed of the rotor of the
Je nach Ausführungsform können die zweite Trommel 106 und/oder die dritte Trommel 110 ortsfest oder beweglich in Bezug auf das Gehäuse 102 vorgesehen sein. Die Trommeln 106, 110 können beispielweise jeweils mittels eines weiteren Aktors drehbar um die Mittelachse 104 vorgesehen sein.Depending on the embodiment, the
Weiterhin kann die Mischkammer 124 eine nicht dargestellte Hindernisstruktur, beispielsweise ein Sieb oder eine Gitterstruktur, aufweisen, welche dazu eingerichtet ist, unter Einwirkung einer Zentrifugalkraft (wenn also die Drehzahl der Zentrifuge einen vorbestimmten Schwellwert überschreitet) sich durch die Flüssigkeiten 502, 504 zu bewegen, um diese dadurch zu vermischen.Furthermore, the mixing
Das Gehäuse 102 und die Trommeln 106, 108, 110 können aus demselben oder unterschiedlichen Polymeren hergestellt sein. Bei dem einen oder mehreren Polymeren handelt es sich insbesondere um Thermoplaste, Elastomere oder thermoplastische Elastomere. Beispiele sind Cyclo-olefin Polymer (COP), Cyclo-olefin Copolymer (COC), Polycarbonate (PC), Polyamide (PA), Polyurethane (PU), Polypropylen (PP), Polyethylene terephthalate (PET) oder Poly(methyl methacrylate) (PMMA).The
Die zweite Trommel 106 und/oder die dritte Trommel 110 können einstückig mit dem Gehäuse 102 gebildet sein.The
Obwohl die Erfindung vorliegend anhand bevorzugter Ausführungsbeispiele beschrieben wurde, ist sie darauf keineswegs beschränkt, sondern auf vielfältige Weise modifizierbar. Insbesondere sei darauf hingewiesen, dass die für die erfindungsgemäße Kartusche vorliegend beschriebenen Ausgestaltungen und Ausführungsbeispiele entsprechend auf die erfindungsgemäße Zentrifuge sowie auf das erfindungsgemäße Verfahren zum Mischen einer ersten und einer zweiten Komponente anwendbar sind, und umgekehrt. Ferner wird darauf hingewiesen, dass "ein" vorliegend keine Vielzahl ausschließt.Although the invention has been described herein with reference to preferred embodiments, it is by no means limited thereto, but modifiable in many ways. In particular, it should be pointed out that the embodiments and exemplary embodiments described herein for the cartridge according to the invention can be applied correspondingly to the centrifuge according to the invention and to the method according to the invention for mixing a first and a second component, and vice versa. It is further noted that "a" in the present case does not exclude a plurality.
Claims (15)
eine erste Trommel (108), welche eine erste Kammer (124) aufweist, und
eine Verstelleinrichtung (300), welche dazu eingerichtet ist, die erste Trommel (108) um deren Mittelachse (104) zu drehen, um dadurch die erste Kammer (124) wahlweise mit einer zweiten oder einer dritten Kammer (120, 122, 132, 136) flüssigkeits-, gas- und/oder partikelleitend zu verbinden,
oder
eine erste Trommel (108), welche eine erste und eine zweite Kammer (124, 126) aufweist, und eine Verstelleinrichtung (300), welche dazu eingerichtet ist, die erste Trommel (108) um deren Mittelachse (104) zu drehen, um dadurch wahlweise die erste Kammer (124) mit einer dritten Kammer (120) oder die zweite Kammer (122) mit der dritten Kammer (120) flüssigkeits-, gas- und/oder partikelleitend zu verbinden,
wobei die Verstelleinrichtung (300) einen Aktor (139) zum Drehen der ersten Trommel um die Mittelachse (104) aufweist.Cartridge (100) for insertion into a centrifuge (1200) and centrifuging it, comprising
a first drum (108) having a first chamber (124), and
an adjusting device (300) adapted to rotate the first drum (108) about its central axis (104), thereby selectively displacing the first chamber (124) with a second or third chamber (120, 122, 132, 136) ) to connect liquid, gas and / or particles,
or
a first drum (108) having first and second chambers (124, 126) and an adjusting device (300) adapted to rotate the first drum (108) about the central axis (104) thereof to thereby rotate selectively connecting the first chamber (124) to a third chamber (120) or the second chamber (122) to the third chamber (120) liquid-, gas- and / or particle-conducting,
wherein the adjusting means (300) comprises an actuator (139) for rotating the first drum about the central axis (104).
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102011077124A DE102011077124A1 (en) | 2011-06-07 | 2011-06-07 | Cartridge, centrifuge and process |
Publications (3)
Publication Number | Publication Date |
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EP2532426A2 true EP2532426A2 (en) | 2012-12-12 |
EP2532426A3 EP2532426A3 (en) | 2013-03-27 |
EP2532426B1 EP2532426B1 (en) | 2015-11-18 |
Family
ID=46027604
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EP12163519.7A Not-in-force EP2532426B1 (en) | 2011-06-07 | 2012-04-10 | Cartridge for insertion into a centrifuge, centrifuge and method |
Country Status (4)
Country | Link |
---|---|
US (1) | US9272278B2 (en) |
EP (1) | EP2532426B1 (en) |
CN (1) | CN102814242A (en) |
DE (1) | DE102011077124A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2532427A3 (en) * | 2011-06-07 | 2013-03-27 | Robert Bosch Gmbh | Cartridge, centrifuge and method |
EP2532428A3 (en) * | 2011-06-07 | 2013-04-03 | Robert Bosch Gmbh | Cartridge, centrifuge and method for mixing a first and a second component |
EP2754494A1 (en) * | 2013-01-14 | 2014-07-16 | Robert Bosch Gmbh | Cartridge, centrifuge for this cartridge and method |
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US9308508B2 (en) * | 2013-07-22 | 2016-04-12 | Kianoosh Peyvan | Sequential delivery device and method |
US10112199B2 (en) | 2014-12-03 | 2018-10-30 | Fiberlite Centrifuge, Llc | Centrifuge sample container and closure therefore |
US9987634B2 (en) | 2014-12-03 | 2018-06-05 | Fiberlite Centrifuge, Llc | Centrifuge sample container and closure therefor |
USD777941S1 (en) | 2015-07-17 | 2017-01-31 | Fiberlite Centrifuge, Llc | Centrifuge bottle |
DE102017115191A1 (en) * | 2017-07-06 | 2019-01-10 | A. u. K. Müller GmbH & Co. KG | Valve, in particular servo valve |
KR102028953B1 (en) * | 2019-02-01 | 2019-10-07 | 비아로지스 주식회사 | Biological Material Manufacturing Device and Driving Method Thereof |
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DE102006003532A1 (en) | 2006-01-24 | 2007-07-26 | INSTITUT FüR MIKROTECHNIK MAINZ GMBH | Centrifugal separation of microlitre liquid quantities of biochemical or medical samples uses rotor and stator chip modules in a 'lab on chip' arrangement |
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FR2665378B1 (en) * | 1990-08-03 | 1992-10-09 | Guigan Jean | DEVICE FOR SEPARATING BY CENTRIFUGATION TWO PHASES FROM A SAMPLE OF A HETEROGENEOUS LIQUID, USABLE IN PARTICULAR FOR THE SEPARATION OF TOTAL BLOOD PLASMA. |
BR9509860A (en) * | 1994-12-02 | 1997-09-30 | Squibb & Sons Inc | Centrifuge reagent delivery system |
US5733446A (en) * | 1994-12-02 | 1998-03-31 | Bristol-Myers Squibb Company | Centrifuge with annular filter |
US5707331A (en) * | 1995-05-05 | 1998-01-13 | John R. Wells | Automatic multiple-decanting centrifuge |
CA2559778C (en) * | 2004-03-26 | 2011-07-05 | Infectio Recherche Inc. | Removable microfluidic flow cell |
DE102004023427A1 (en) | 2004-05-09 | 2005-12-22 | Microfluidic Chipshop Gmbh | Connector-distributor for chip systems, used e.g. in transport or analysis of fluids, includes luer connector and penetrations for multiple fluid channels in chip component |
JP2008224383A (en) | 2007-03-12 | 2008-09-25 | Olympus Corp | Reagent storage and autoanalyzer |
JP5277245B2 (en) | 2008-05-22 | 2013-08-28 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
DE102011007779A1 (en) * | 2011-04-20 | 2012-10-25 | Robert Bosch Gmbh | Mixing chamber, cartridge and method for mixing a first and second component |
EP2532458B1 (en) | 2011-06-06 | 2017-03-29 | HILTI Aktiengesellschaft | Threaded bar deburrer |
DE102011077134A1 (en) * | 2011-06-07 | 2012-12-13 | Robert Bosch Gmbh | Cartridge, centrifuge and method for mixing a first and second component |
DE102011077115A1 (en) * | 2011-06-07 | 2012-12-13 | Robert Bosch Gmbh | Cartridge, centrifuge and process |
DE102011077101A1 (en) * | 2011-06-07 | 2012-12-13 | Robert Bosch Gmbh | Microfluidic system and method of operating such a system |
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2011
- 2011-06-07 DE DE102011077124A patent/DE102011077124A1/en not_active Withdrawn
-
2012
- 2012-04-10 EP EP12163519.7A patent/EP2532426B1/en not_active Not-in-force
- 2012-06-06 CN CN2012101840040A patent/CN102814242A/en active Pending
- 2012-06-07 US US13/490,942 patent/US9272278B2/en not_active Expired - Fee Related
Patent Citations (1)
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DE102006003532A1 (en) | 2006-01-24 | 2007-07-26 | INSTITUT FüR MIKROTECHNIK MAINZ GMBH | Centrifugal separation of microlitre liquid quantities of biochemical or medical samples uses rotor and stator chip modules in a 'lab on chip' arrangement |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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EP2532427A3 (en) * | 2011-06-07 | 2013-03-27 | Robert Bosch Gmbh | Cartridge, centrifuge and method |
EP2532428A3 (en) * | 2011-06-07 | 2013-04-03 | Robert Bosch Gmbh | Cartridge, centrifuge and method for mixing a first and a second component |
US9399214B2 (en) | 2011-06-07 | 2016-07-26 | Robert Bosch Gmbh | Cartridge, centrifuge and method |
US9475043B2 (en) | 2011-06-07 | 2016-10-25 | Robert Bosch Gmbh | Cartridge, centrifuge and method for mixing a first and second component |
EP2754494A1 (en) * | 2013-01-14 | 2014-07-16 | Robert Bosch Gmbh | Cartridge, centrifuge for this cartridge and method |
Also Published As
Publication number | Publication date |
---|---|
EP2532426A3 (en) | 2013-03-27 |
CN102814242A (en) | 2012-12-12 |
US20120316050A1 (en) | 2012-12-13 |
DE102011077124A1 (en) | 2012-12-13 |
US9272278B2 (en) | 2016-03-01 |
EP2532426B1 (en) | 2015-11-18 |
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