EP2492015B1 - Porte-échantillon, système et procédé d'analyse - Google Patents

Porte-échantillon, système et procédé d'analyse Download PDF

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Publication number
EP2492015B1
EP2492015B1 EP20120157292 EP12157292A EP2492015B1 EP 2492015 B1 EP2492015 B1 EP 2492015B1 EP 20120157292 EP20120157292 EP 20120157292 EP 12157292 A EP12157292 A EP 12157292A EP 2492015 B1 EP2492015 B1 EP 2492015B1
Authority
EP
European Patent Office
Prior art keywords
sample
drill
sample container
container
septum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP20120157292
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German (de)
English (en)
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EP2492015A1 (fr
Inventor
Bernhard Hukelmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DIL Deutsches Institut fuer Lebensmitteltechnik eV
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DIL Deutsches Institut fuer Lebensmitteltechnik eV
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Publication of EP2492015A1 publication Critical patent/EP2492015A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure

Definitions

  • the present invention relates to a sample container and a system of devices for analysis, and a method for analysis with the sample container.
  • the sample container is characterized by an integrated device for sampling and is adapted to the system for analysis, or to the method of analysis, which is preferably automated.
  • the sample container is adapted by the sampling device without additional handling device or tool. Accordingly, the sample container consisting of a combination with the sampling device is suitable for use as a sampler.
  • sample material adapted to the processing process must be present in the sample bag.
  • sample quantity must not be exceeded. If this is the case, frequently a corresponding proportion by weight of the sample under sterile conditions must often be taken from the plastic bag so that only the admissible proportion remains or is further processed.
  • the bags containing the samples are often added with a weight-corresponding amount of medium (e.g., culture medium or water) and then subjected to a homogenization step. After the crushing of the sample material, either the entire bag with the contents or a part thereof is fed to an incubation step. Finally, taking into account further method steps, if appropriate, the corresponding analysis is carried out with e.g. a PCR.
  • medium e.g., culture medium or water
  • the DE 3407646 A1 describes a device for taking samples of material.
  • a round cutting cylinder at the rear end according to an embodiment, a collecting container is releasably secured, is rotatably mounted about its longitudinal axis on a holding forceps.
  • the round cutting cylinder is rotated by the holding forceps, whereby a clean cutting action is achieved.
  • the cut-out material sample can be transferred to the collection container, which is then released from the holding forceps.
  • the DE 19740429 A1 describes an apparatus and a method for taking biological samples in very large numbers, coupled with an immediate registration of the sample data.
  • the tissue samples are removed by punching, scraping, or cutting with a portion of a sample capsule, which is pressed against the sample material with forceps and then directly merged with a second portion to form a closed sample capsule.
  • the sample capsule may have a septum.
  • the DE 196 08 110 A1 describes a container for the removal, storage and processing of samples with a plastic bag, at the opening of a nozzle is welded. At the neck a cap is molded, with which the neck can be closed. For sample processing, a filter attachment can be arranged on the nozzle.
  • the object of the invention is to provide an alternative sample container which has an integrated device for sampling and in which the subsequent analysis can be prepared, in particular that individual steps of sample preparation and / or analysis are carried out in the sample container.
  • Another object of the present invention is to provide a system and a method of analysis using the sample container, wherein the system and the method performed therewith are preferably automatable.
  • the sample container is suitable for sampling without the use of pliers.
  • the invention provides a sample container as a device for sampling, an analysis method which can be carried out with the sample container, preferably automatically controlled, and an analysis system tuned to the sample container, which preferably has a control device, and preferably an analysis method which can be carried out with the analysis system and is automatically controlled by a control device.
  • the control device is set up to control the devices of the analysis system according to the steps of the analysis method.
  • the invention provides a sample container adapted to an automated analysis method and adjacent to a label, preferably by machine can be read, for example in the form of a bar code and / or a transponder, and additionally comprises a human-readable labeling, for example, an alphanumeric label, a deformable, elastic plastic bag which is attached to a plastic carrier.
  • a human-readable labeling for example, an alphanumeric label, a deformable, elastic plastic bag which is attached to a plastic carrier.
  • the label may include data that individualize a sample container so that labeling is not required during sampling, but the method only associates the data of the label with the sample, eg location and time of sampling, for example, by inputting these data into a first central computer, preferably in that the central computer is set up to associate respectively generated current data with a label detected by a reading unit, eg current time and a preselected location of the sampling and / or preselected data on the nature of the sample material.
  • the central computer can be part of the control unit of the analysis system.
  • the plastic carrier allows easy handling by an automated analysis method, which includes, for example, an automated delivery of the sample container by means of grabbing on the plastic carriers gripper and / or homogenization of the sample by mechanical loading and unloading, which is particularly cyclical, of the elastic plastic bag.
  • the sample drill protrudes beyond the carrier on the side of the plastic carrier facing away from the elastic plastic bag and has an access opening and a space spaced apart from the access opening by the sample drill, e.g. this opposite, the bag-facing inner opening, wherein the sample drill preferably with its end facing the bag, the plastic carrier does not exceed and the inner opening of the sample drill is located in a side facing the bag of the plastic carrier.
  • the edge of the sample drill which surrounds the access opening is preferably formed as a cutting edge.
  • the sample container according to the invention is further distinguished by the fact that a sample drill, also called a sample cutter, is attached to the plastic carrier in a liquid-tight and gas-tight manner and a septum can be fixed to the plastic carrier, the plastic bag or the sample drill in a liquid-tight manner, or at least in the closed state of Sample container is fixed, for example by means of the lid.
  • the sample drill has at least one retaining element on its inner wall, which allows sample material inserted into the sample drill into the bag and prevents sample material from slipping out of the access opening.
  • the sample drill has two retaining elements, which are arranged on opposite inner walls of the sample drill.
  • a retaining element may be formed by one or more cutting lips, eg at least one elastic wing or pin projecting beyond the inner wall of the sample drill, and / or by an inner spiral which engages with the sample material.
  • the retaining element leads to the cutting or tearing off of sample material from a larger piece to be sampled.
  • an advantage of the sample container according to the invention is that with its sample drill even without complete piercing of the material to be sampled a sample is obtained, since the retaining element causes the separation of the sample material, which is in the sample drill.
  • a retaining element In the unloaded state, such a retaining element preferably reduces the internal cross section of the sample drill from the access opening to the inner opening facing the bag and, as sample material passes through the sample drill, it is pressed towards its inner opening in the direction of the inner wall of the sample drill and prevents the sample material from slipping back or slipping out the access opening.
  • the retaining element may be an elastic element which, in the unloaded state, at least partially, preferably completely covers the cross-section of the sample drill and which is articulated or fixed at one end within the sample drill and whose opposite free end is arranged closer to the inner opening of the sample drill.
  • the retention member may be rigid, hinged at one end in the sample drill and loaded with a spring member toward an opposing portion of the sample drill with the hinged end opposite free end of the retention member facing the access port of the sample drill or closer than the hinged end is arranged at the inner opening of the sample drill.
  • a retaining element may be, for example, an elastically movable or elastically deformable wing which, in the unloaded state, covers the cross section of the sample drill and faces the free end of the access opening of the sample drill.
  • the fixed end opposite the fixed end of the retaining element has a cutting edge.
  • the internal volume of the sample drill is According to the invention preferably chosen so that the inner volume of the sample drill is not more than 10% of the maximum volume of the plastic bag, so that a sample separated with the sample drill and analyzed sample in the elastic plastic bag does not occupy more than 10% of its volume and still sufficient space for glades Medium remains.
  • the internal volume of the sample drill is preferably adapted to a product-typical removal quantity.
  • the sample container for use of the sample container as a sampler and for receiving sample material, in particular without pliers, the sample container of a plastic elastic bag which is provided with a label and a liquid-tight fastened thereto by means of a carrier sample drill, which spans an access opening and having a lid, which reversibly closes the access opening liquid-tight, wherein the elastic plastic bag is attached liquid-tight at the spaced from the access opening of the inner opening of the sample drill by means of the carrier, the sample drill at least one retaining element for sample material and preferably a septum liquid-tight thereby arranged in the sample container that it in the Sample drill, is embedded in the lid or in the carrier, through which the inner volume of the elastic plastic bag is accessible.
  • the retaining element is preferably hinged at one end in the sample drill and its opposite end disposed away from the access opening and preferably has a cutting edge.
  • the access opening of the sample drill is preferably closed by a detachably arranged on this cover, which preferably in the closed state on the inner and / or outer wall of the sample drill liquid-tight, closed.
  • the lid may be separate from the sample container prior to use of the sample container, but preferably the lid is releasably disposed on the access opening and seals it in a liquid-tight manner.
  • the lid is connected to the sample container, for example, connected by a band or web, which is formed for example in one piece with the lid and / or carrier.
  • An associated with the sample container lid has the advantage that it is located immediately after sampling in close proximity to the sample drill and allows easy handling, the closing of the sample drill.
  • the lid can be screwed or clamped on the sample drill, for example, with the inner and / or outer wall of the sample drill in elastic engagement.
  • the septum and the sample drill may be spaced from each other on the same plastic carrier or disposed on different plastic supports so that the interior volume of the bag is accessible through the sample drill and the septum spaced apart.
  • the lid is detachably mounted on the access opening and is removed for sampling from the access opening and then placed back on it to close the sample container to the sampling and after sampling liquid-tight, preferably sterile.
  • the septum may be integral with the cover fluid-tightly surrounding the access port of the sample drill, such that the interior volume of the bag is accessible through the septum and the sampler when the cover has an integral septum which closes the access port of the sampler after sampling ,
  • the septum can optionally be embedded directly in the lid and in particular form the lid portion which completely or partially covers the opening of the sample drill.
  • the lid may include at least one elongated member positioned within the sample drill when the lid is placed on the access opening, preferably wherein the septum is disposed in a portion of the lid which covers the access opening of the sample drill and adjacent to the elongate member, or next to the end face of the stretched element.
  • the elongated member may include one or more pins projecting beyond the inner surface of the lid, and further optionally forming a fork-shaped tail suitable for, for example, spiking sample material.
  • the elongate member is a length of pipe which is inserted longitudinally in the sample drill in the closed state of the sample container, wherein the septum is arranged in the cover portion which covers the clear cross-section of this pipe piece.
  • the stretched element When the sample drill is closed by placing the cover over the access opening, the stretched element is inserted into the sample drill and displaces the retaining element so that the inner volume of the bag becomes accessible next to the stretched element.
  • the internal volume is accessible through a septum mounted in the cover, which covers the cross-sectional area of the tube forming the elongated element and disposed in the sample drill, pushing the at least one retention element against the wall of the sample drill.
  • the sample drill has a predetermined internal volume, for example a predetermined length between the retaining element and the access opening and / or between the retaining element and the inner opening.
  • the sample drill is adapted to separate a predetermined sample volume, eg, the internal volume of the sample drill may be the full sample volume or one half, one third, one fourth or less of the predetermined sample volume.
  • the sample drill allows the separation of a predetermined sample volume by simply or repeatedly piercing the sample drill into the material to be sampled and avoids, for example, a later portioning of the sample material received in the sample container.
  • the plastic carrier is tightly connected to the elastic plastic bag, for example, welded or glued to the bag, or the bag is jammed with the plastic carrier.
  • the plastic carrier may comprise at least one projection, preferably two opposing projections which optionally project beyond the circumference of the bag and / or project beyond the circumference of the sample drill, so that the sample bag can be held at the neck, for example at a receiving opening of a transport box, in which one or several test bags are arranged.
  • the approach allows the approach to engage a gripper and be handled by the gripper, which may be part of a system for analysis.
  • the elastic plastic bag at a distance from the plastic carrier, in particular with respect to the plastic carrier, on a visible scale, with which the volume of a sample located in the bag can be estimated.
  • the sample drill which preferably has a round profile inside and / or outside, can be arranged with its longitudinal axis perpendicular to the plastic carrier, in particular with its longitudinal axis approximately perpendicular to a hand support surface of an optionally fixed to the carrier handle, or parallel to the longitudinal axis of a handle.
  • An optional grip with a hand rest surface may be integral with the carrier and / or with the sample drill or engage the shoulder.
  • the inner surface of the sampler may have a constant cross section over the length of the sampler;
  • the sample drill has a cross-section increasing from its access opening to the inner opening facing the bag on, for example, a frustoconical widening of the access opening cross-section.
  • the sample drill has a helical guide which, upon rotation of the sample drill into a mass to be sampled, causes delivery of the sample material into the sample drill and / or engages sample material and, optionally in conjunction with the cutting edge of the sample drill, causes a tearing of the sample material.
  • the lid preferably has a peripheral rim which abuts the inner surface and / or the outer surface in a portion of the sample drill adjacent to the access opening and, when placed on the access opening, seals it in a liquid-tight manner.
  • the support, the lid and / or the sample drill has a window that is transparent to radiation in the range of 150 to 850 nm, in particular from 200 to 800 nm or 400 to 800 nm, and e.g. made of plastic or glass.
  • the window is arranged in the carrier.
  • Such an optically transparent window is suitable for optical analysis methods, e.g. for use with an analysis system in which optical radiation is radiated through the window and e.g. the simultaneously or temporally staggered radiation emerging from the sample container is detected.
  • the analysis system comprises a radiation source, the beam of which is directed to the window of the sample container held in the system, and a detector adapted to measure radiation exiting the window, in particular for detecting the number of microorganisms based on the measured exiting radiation.
  • a detector may e.g. be arranged for the detection of fluorescence radiation emitted by sample material in the medium, in particular after irradiation of light of an excitation wavelength.
  • the analysis system is automated, e.g. by a controller arranged to control each device or component of the system for the analysis method.
  • the sample container has a sensor contacting its internal volume, so that the sensor is introduced from sample material and / or into the sample container Medium is contacted.
  • the sensor is preferably arranged on the surface of the carrier or of the sample drill facing the internal volume of the sample container.
  • a sensor is preferably electronically or optically readable by the analysis system and may be a pH sensor, a conductivity or impedance sensor and / or, for example, have a dye whose optical properties change specifically in contact with the sample material and / or medium, for example by color change ,
  • the volume of the elastic plastic bag is preferably at least 5-20 larger than the volume of the sample drill between the access opening and the inner opening, preferably by a factor of 8-10.
  • the elastic plastic bag is uniquely identifiable by virtue of its labeling, which is preferably human-readable and additionally machine-readable, with the fixed connection of the sample drill to the elastic plastic bag ensuring that the sample is attached to the label as soon as it is removed.
  • the sample container according to the invention allows the immediate removal, for example by scraping, cutting, etc., as well as the packaging of the sample material to be analyzed with the same sample container, i. without additional tools.
  • the analysis method may be performed with the step of taking out sample material in which the device for taking sample material from the sample container is made, and the system for analysis has as a device for taking sample material a device consisting of the sample container.
  • FIG. 1 shows a sample container 1 with a flexible plastic bag 2, which carries a label 3 and is attached to a plastic carrier
  • the label 3 can be one or more parts and have machine-readable labeling, for example a transponder or bar code, and human-readable alphanumeric labeling.
  • the retaining element 14 has at its end opposite the hinged end a cutting edge 21 which faces away from the access opening 7.
  • the second end 8 of the sample drill 5 is connected via the inner opening 9 with the inner volume of the elastic plastic bag 2.
  • the inner opening is flush in the bag 2 facing surface of the support 4 embedded.
  • the sample container 1 is provided according to a preferred embodiment on its elastic plastic bag 2 with a scale 15, with which the volume of a sample material located in the elastic plastic bag 2 can be estimated.
  • the scale 15 may extend diagonally across a corner of the bag 2 or parallel to an edge of the bag 2.
  • a septum 11 is in this embodiment, spaced from the sample drill 5 so mounted on the plastic support 4, that when piercing the septum 11 with a hollow needle or a tube, the inner volume of the elastic plastic bag 2 is accessible. Through the septum 11 liquids can be supplied to the bag 2 and / or removed by means of a tube, wherein the septum 11 closes automatically after removal of the tube.
  • the carrier 4 forms lugs 12 which protrude above the bag 2 and can be handled by a mechanical gripper.
  • An optically transparent window 19 is disposed in the support 4 and permits the irradiation of light of an excitation wavelength and the emission of radiation from the pouch 2.
  • a sensor 20 is additionally shown, e.g. is a pH-sensitive indicator whose color change is optically detectable through the window 19.
  • FIG. 2 shows a sample drill 5, at the first end 6 of the access opening 7 is clamped.
  • the second end 8 of the sample drill 5 biases the inner opening 9, which is connected to the inner volume of the bag 2.
  • the inner surface of the sample drill 5 can have a constant over the length of the sample drill 5 in cross-section.
  • two elastic wings are attached as a retaining element 14.
  • FIG. 3 shows a further embodiment of the sample drill 5, in which on the inner surface of the sample drill 5, a helical guide 13 is mounted.
  • FIG. 4 shows a lid 10, which is adapted to close the access opening 7 of the sample drill 5 liquid-tight.
  • the lid 10 has a cylindrical, elongated element 17, which is preferably arranged at right angles to the main surface of the lid 10 and / or parallel to the longitudinal axis of the sample drill 5.
  • FIG. 5 shows the access opening 7 of the sample drill 5 with a lid 10 which is connected by means of a belt 18 to the carrier 4.
  • the outer diameter of the elongated element 17 is according to the invention smaller than the inner diameter of the sample drill 5, but so large that the arranged on the inner surface of the sample drill 5 retaining element 14 when closing the access opening 7 through the lid 10 in the direction of the elastic plastic bag 2 and against the Inner wall of the sample drill 5 are pressed.
  • the septum 11 forms a portion of the lid 10 which closes the elongate element 17, and e.g. in the embodiment of the elongated element 17 as a piece of pipe covers the opening which is attached to the cover 10.
  • the sample container 1 has a handle 16 which is attached to the sample drill 5, e.g. is fixed to the lugs 12.
  • the handle 16 is detachably connected to the test tap 5, in particular to the projections 12 arranged on the sample drill 5 or on the support 4.
  • a handle 16 may be formed by the peripheral surface of the sample drill 5, which is a hand rest surface.
  • the sample drill 5 can have an outer length of about 8 to 16 cm, in particular of 10 to 12 cm, for example, with an outer diameter of 0.5 to 3 cm.
  • a window 19 is arranged in the sample drill 5, so that sample material and filled medium is arranged in an orientation of the sample drill 5 horizontally to vertically downward in the sample drill 5.
  • FIG. 6 shows a further embodiment of the sample container 1, in which a handle 16 of the access opening 7 of the sample drill 5 is arranged opposite and sample drill 5 and handle 16 preferably have a common axis.
  • the handle 16 has a hand support surface 16 a, which is arranged perpendicular to the longitudinal axis of the sample drill 5.
  • the sample drill 5 optionally has a bent portion which is connected to the bag 2, so that the second end 8 of the sample drill 5 opens with the inner opening 9 in the elastic plastic bag 2.
  • the handle 16 allows easy handling of the sample drill 5 since the bag 2 is connected off-axis to the sample drill 5 to which the access opening 7 and the palm support surface 16a are perpendicular.
  • the carrier 4 may be an integral part of the sample drill 5 and connect to the optional bag 2 and / or the inner opening 9 tapered bag 2.
  • the septum 11 in this embodiment is preferably disposed in a wall of the sample drill 5, and particularly forms a wall portion of the sample drill 5 which is opposed to the inner opening 9 and connected to the inner opening by the volume of the sample drill 5 including its bent portion.
  • a window 19 may optionally be arranged generally in addition to the septum 11 on the sample drill 5, for example next to the septum 11.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (17)

  1. Réceptacle d'échantillons (1) pour application comme organe d'échantillonnage et permettant de recevoir un matériau échantillon, lequel présente une étiquette (3) et contient une ouverture d'accès (7) bridée par un piston à échantillon (5) de même qu'un couvercle (10), lequel couvercle obture l'ouverture d'accès (7) de manière réversible et imperméable au liquide, caractérisé en ce qu'un sac plastique élastique (2) est fixé à l'aide d'un support (4) de manière imperméable au liquide au niveau de l'ouverture interne (9) du piston à échantillon (5) à une certaine distance de l'ouverture d'accès (7), que le piston à échantillon (5) comprend au moins un élément de retenue (14) pour le matériau échantillon et qu'un septum (11) est disposé dans le réceptacle d'échantillons (1) de manière imperméable au liquide de façon à traverser le piston à échantillon (5), le couvercle (10) ou le support (4), et ainsi pouvoir accéder au volume interne du sac plastique élastique (2).
  2. Réceptacle d'échantillons (1) selon la revendication 1, caractérisé en ce que l'élément de retenue (14) est articulé en une extrémité dans le piston à échantillon (5), que son extrémité opposée tourne le dos à l'ouverture d'accès (7) et présente un bord tranchant.
  3. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce que le septum (11) fait partie du couvercle (10).
  4. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce que le piston à échantillon (5) présente une poignée (16) munie d'un repose-main (16a), lequel repose-main est parallèle au plan de l'ouverture d'accès (7).
  5. Réceptacle d'échantillons (1) selon l'une des revendications 1 à 2, caractérisé en ce que le support (4) est partie intégrante du piston à échantillon (5) et qu'une poignée (16) est constituée de la surface périphérique du piston à échantillon (5).
  6. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce que le piston à échantillon (5) présente une section recourbée et que le septum (11) forme une section de la paroi du piston à échantillon (5) faisant face à l'ouverture interne (9).
  7. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce qu'un élément étiré (17) est fixé sur la face du couvercle (10) opposée à l'ouverture d'accès (7) alors fermée, élément d'une longueur recouvrant au moins l'intervalle entre l'ouverture d'accès (7) et l'élément de retenue (14).
  8. Réceptacle d'échantillons (1) selon la revendication 6, caractérisé en ce que l'élément étiré (17) est un morceau de tuyau dont la section transversale jouxtant le couvercle (10) est recouverte par le septum (11).
  9. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce qu'une fenêtre optiquement transparente (19) et/ou un capteur (20) est disposé dans le support (4).
  10. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce que le support (4) présente au moins un insert (12) au niveau duquel une poignée (16) est fixée.
  11. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce que l'ouverture d'accès (7) du piston à échantillon (5) est entourée d'un bord tranchant.
  12. Réceptacle d'échantillons (1) selon l'une des revendications précédentes, caractérisé en ce que le piston à échantillon (5) présente un guidage spiralé (13) sur sa face interne, guidage s'engrenant avec un matériau échantillon dans le piston à échantillon (5) et/ou que la section transversale interne du piston à échantillon (5) augmente de l'ouverture d'accès (7) à l'ouverture interne (9).
  13. Système d'analyse présentant au moins un réceptacle d'échantillons (1) selon l'une des revendications précédentes, dont l'étiquette comprend une étiquette lisible électroniquement et une étiquette visible alphanumérique, englobant
    - une unité de lecture électronique permettant de détecter l'étiquette lisible électroniquement,
    - un premier ordinateur central relié à l'unité de lecture électronique et
    - un second ordinateur relié au premier ordinateur central pour assurer la transmission des données,
    - un dispositif d'analyse disposant d'une balance permettant de peser le réceptacle d'échantillons et de calculer la masse du matériau échantillon,
    - un dispositif de remplissage, qui est conçu pour le remplissage d'un volume de liquide en suspension calculé en fonction de la masse du matériau échantillon, dans le réceptacle d'échantillons (1) à travers un premier petit tube passant par le septum (11),
    - un dispositif de mélange, conçu pour la contrainte mécanique du sac (2) pour assurer le mélange et l'homogénéisation au moins partielle du matériau échantillon dans le liquide en suspension,
    - un dispositif de prélèvement muni d'un second petit tube, conçu pour le prélèvement d'une proportion en volume du liquide en suspension prédéterminée à travers le second petit tube passant par le septum (11), et conçu pour doser au moins un aliquote du liquide en suspension prélevé dans le médium de culture liquide et/ou sur un médium de culture solide,
    - un incubateur pour incuber le médium de culture,
    - une unité de détection permettant de déterminer le nombre de micro-organismes dans le liquide en suspension et/ou dans le médium de culture liquide et/ou sur le médium de culture solide, où l'unité de détection possédant une conduite de données est reliée au second ordinateur, lequel est conçu pour affecter des données détectées à un étiquetage détecté et lisible électroniquement et également conçu pour assurer la transmission des données affectées à l'étiquetage au premier ordinateur central.
  14. Système d'analyse selon la revendication 13, caractérisé en ce que le réceptacle d'échantillons (1) présente une fenêtre optiquement transparente (19) et que l'unité de détection présente un détecteur optique captant le rayonnement sortant par la fenêtre (19).
  15. Système selon l'une des revendications 13 à 14, caractérisé par une unité de commande qui est conçue pour commander le dispositif de remplissage afin de remplir le volume calculé de liquide en suspension, qui est conçue pour commander le dispositif de mélange, qui est conçue pour commander le dispositif de prélèvement afin de prélever une proportion en volume prédéterminée du liquide en suspension, qui est conçue pour commander l'incubateur pour incuber et qui est conçue pour commander l'unité de détection afin de déterminer le nombre de micro-organismes.
  16. Procédé d'analyse comprenant les phases de préparation d'un réceptacle d'échantillons selon l'une des revendications 1 à 11,
    - de prise en charge du matériau échantillon avec l'ouverture d'accès (7) du piston à échantillon (5), où le réceptacle d'échantillons (1) est maintenu au niveau d'une poignée (16) fixée sur le support (4) et le matériau échantillon glisse dans le sac plastique (2) par l'intermédiaire d'au moins un élément de retenue (14),
    - de fermeture du réceptacle d'échantillons (1) par agencement du couvercle (10) sur l'ouverture d'accès (7), réceptacle présentant un élément étiré (17) qui repousse le matériau échantillon dans le sac plastique (2) lors de la fermeture et l'élément de retenue (14) pèse sur la paroi interne du piston à échantillon (5),
    - de lecture de l'étiquette lisible électroniquement dans un premier ordinateur central et transmission du nombre de réceptacles d'échantillons (1) vers un second ordinateur, relié à l'unité d'interprétation d'un dispositif d'analyse,
    - de transport du réceptacle d'échantillons (1) au dispositif d'analyse,
    - de pesée du réceptacle d'échantillons (1) et calcul de la masse du matériau échantillon,
    - de remplissage d'un volume de liquide en suspension calculé en fonction de la masse du matériau échantillon dans le réceptacle d'échantillons (1) à travers un premier petit tube passant par le septum (11),
    - de contrainte mécanique du sac plastique (2) pour assurer le mélange et l'homogénéisation au moins partielle du matériau échantillon dans le liquide en suspension,
    - de prélèvement d'une proportion en volume prédéterminée du liquide en suspension à travers un second petit tube passant par le septum (11),
    - d'incubation d'un aliquote du liquide en suspension prélevée dans un médium de culture liquide et/ou d'étalement d'un aliquote du liquide en suspension prélevé sur au moins un médium de culture solide avec incubation subséquente,
    - de détection du nombre de micro-organismes dans le médium de culture liquide et/ou sur le médium de culture solide et
    - d'affectation du nombre détecté de micro-organismes aux données du matériau échantillon mémorisés dans le second ordinateur, et
    - de transmission des données associées au matériau échantillon dans le second ordinateur vers le premier ordinateur central.
  17. Procédé selon la revendication 16, caractérisé en ce que le piston à échantillon (5) est guidé dans un matériau à échantillonner et que le matériau échantillon est maintenu à travers l'élément de retenue (14) après prise en charge avec l'ouverture d'accès (7) et est séparé exclusivement par arrachement à l'intérieur du piston à échantillon (5) et/ou par découpe le long d'un bord tranchant de l'élément de retenue (14).
EP20120157292 2011-02-28 2012-02-28 Porte-échantillon, système et procédé d'analyse Active EP2492015B1 (fr)

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DE102015223791A1 (de) 2015-11-30 2017-06-01 Bernhard Hukelmann Probenbeutel für automatisiertes Analyseverfahren

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CN109580277B (zh) * 2018-12-20 2024-03-12 北京英斯派克科技有限公司 一种固体材料全自动取样装置及方法
CN110250164A (zh) * 2019-07-19 2019-09-20 上海长征医院 一种肝脏移植过程中的低温保护袋
USD1014780S1 (en) 2022-04-15 2024-02-13 Instrumentation Laboratory Co. Cuvette

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DE3407646A1 (de) 1984-03-01 1985-09-05 Gottfried Prof. Dr. 8057 Eching Pfeiffer Vorrichtung zur entnahme von materialproben
DE19608110B4 (de) * 1996-03-02 2006-01-12 Undine Dr. Soltau Verwendung eines Probenbehältnisses
DE19740429C2 (de) 1997-09-11 2002-03-14 Biopsytec Gmbh Verfahren zur Entnahme von biologischen Proben
WO2008003693A1 (fr) * 2006-07-04 2008-01-10 Tecan Trading Ag Dispositif de collecte pour des échantillons pertinents d'un point de vue biologique
DE102008034677B4 (de) * 2008-07-25 2012-05-16 Gepe-Geimuplast Gmbh Kennzeichnungs- und Probenahmevorrichtung für Tiere

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DE102015223791A1 (de) 2015-11-30 2017-06-01 Bernhard Hukelmann Probenbeutel für automatisiertes Analyseverfahren
DE102015223791B4 (de) 2015-11-30 2019-02-07 Bernhard Hukelmann Probenbeutel für automatisiertes Analyseverfahren sowie Verfahren zur Analyse

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