EP2300613A4 - Mutants d'adn polymérases thermiquement stables modifiés chimiquement - Google Patents
Mutants d'adn polymérases thermiquement stables modifiés chimiquementInfo
- Publication number
- EP2300613A4 EP2300613A4 EP09767773A EP09767773A EP2300613A4 EP 2300613 A4 EP2300613 A4 EP 2300613A4 EP 09767773 A EP09767773 A EP 09767773A EP 09767773 A EP09767773 A EP 09767773A EP 2300613 A4 EP2300613 A4 EP 2300613A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- mutated
- chemically modified
- thermally stable
- dna polymerases
- stable dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7375408P | 2008-06-18 | 2008-06-18 | |
PCT/US2009/047862 WO2009155464A2 (fr) | 2008-06-18 | 2009-06-18 | Mutants d'adn polymérases thermiquement stables modifiés chimiquement |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2300613A2 EP2300613A2 (fr) | 2011-03-30 |
EP2300613A4 true EP2300613A4 (fr) | 2011-11-09 |
Family
ID=41434700
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP09767773A Withdrawn EP2300613A4 (fr) | 2008-06-18 | 2009-06-18 | Mutants d'adn polymérases thermiquement stables modifiés chimiquement |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP2300613A4 (fr) |
WO (1) | WO2009155464A2 (fr) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010062777A2 (fr) | 2008-11-03 | 2010-06-03 | Kapabiosystems | Adn polymérases de type a modifiées |
US9315787B2 (en) | 2011-01-14 | 2016-04-19 | Kapa Biosystems, Inc. | Modified DNA polymerases for improved amplification |
WO2013008898A1 (fr) * | 2011-07-12 | 2013-01-17 | 国立大学法人九州大学 | Nouvelle adn polymérase |
CN104619853B (zh) * | 2012-04-23 | 2021-10-12 | 帝斯曼知识产权资产管理有限公司 | 多肽表达方法 |
DE102012216193B4 (de) | 2012-09-12 | 2020-07-30 | Continental Automotive Gmbh | Verfahren und Vorrichtung zur Bedienung einer Kraftfahrzeugkomponente mittels Gesten |
EP3960760A1 (fr) * | 2015-11-16 | 2022-03-02 | Ubiprotein, Corp. | Procédé de prolongation de la demi-vie d'une protéine |
WO2018203582A1 (fr) * | 2017-05-05 | 2018-11-08 | 주식회사 유비프로틴 | Procédé pour prolonger la demi-vie d'une protéine |
JP7470216B2 (ja) | 2020-05-29 | 2024-04-17 | ジェノテック コーポレイション | 遺伝子変異に対する区分性が向上したdna重合酵素変異体 |
CN115011579B (zh) * | 2022-04-28 | 2024-02-02 | 北京丹大生物技术有限公司 | 一种Taq DNA聚合酶突变体及其制备方法和应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002022869A2 (fr) * | 2000-09-13 | 2002-03-21 | Medical Research Council | Procede d'evolution dirigee |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
KR100624490B1 (ko) * | 2004-05-27 | 2006-09-20 | 바이오퀘스트(주) | 화학변형 열안정성 dna 중합효소 |
-
2009
- 2009-06-18 WO PCT/US2009/047862 patent/WO2009155464A2/fr active Application Filing
- 2009-06-18 EP EP09767773A patent/EP2300613A4/fr not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002022869A2 (fr) * | 2000-09-13 | 2002-03-21 | Medical Research Council | Procede d'evolution dirigee |
Non-Patent Citations (1)
Title |
---|
GHADESSY FARID J ET AL: "Directed evolution of polymerase function by compartmentalized self-replication", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, WASHINGTON, DC; US, vol. 98, no. 8, 10 April 2001 (2001-04-10), pages 4552 - 4557, XP002189673, ISSN: 0027-8424, DOI: 10.1073/PNAS.071052198 * |
Also Published As
Publication number | Publication date |
---|---|
WO2009155464A2 (fr) | 2009-12-23 |
EP2300613A2 (fr) | 2011-03-30 |
WO2009155464A3 (fr) | 2010-05-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20110114 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR |
|
AX | Request for extension of the european patent |
Extension state: AL BA RS |
|
DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20111007 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12N 9/12 20060101ALI20110930BHEP Ipc: C12N 9/10 20060101ALI20110930BHEP Ipc: C12N 15/54 20060101AFI20110930BHEP |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20120505 |