EP2298912A3 - Mikroorganismenstamm zur Produktion von rekombinanten Proteinen - Google Patents

Mikroorganismenstamm zur Produktion von rekombinanten Proteinen Download PDF

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Publication number
EP2298912A3
EP2298912A3 EP10175511A EP10175511A EP2298912A3 EP 2298912 A3 EP2298912 A3 EP 2298912A3 EP 10175511 A EP10175511 A EP 10175511A EP 10175511 A EP10175511 A EP 10175511A EP 2298912 A3 EP2298912 A3 EP 2298912A3
Authority
EP
European Patent Office
Prior art keywords
microorganism strain
recombinant proteins
producing recombinant
mutated
gene encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP10175511A
Other languages
English (en)
French (fr)
Other versions
EP2298912A2 (de
Inventor
Susanne Dr. Leonhartsberger
Günter Dr. Wich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wacker Chemie AG
Original Assignee
Wacker Chemie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wacker Chemie AG filed Critical Wacker Chemie AG
Publication of EP2298912A2 publication Critical patent/EP2298912A2/de
Publication of EP2298912A3 publication Critical patent/EP2298912A3/de
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft einen Mikroorganismenstamm, der ein Gen kodierend ein rekombinantes Protein sowie ein mutiertes Gen kodierend ein Wirtsprotein welches keine Protease ist umfasst, dadurch gekennzeichnet, dass das rekombinante Protein in einer Fermentation sekretiert wird und das mutierte Gen kodierend das Wirtsprotein derart mutiert ist, dass es eine im Vergleich zu dem dem mutierten Gen zugrunde liegenden Wildtyp Gen eine verminderte Expression des Wirtsproteins bewirkt.
EP10175511A 2006-02-02 2007-01-29 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen Ceased EP2298912A3 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006004871A DE102006004871A1 (de) 2006-02-02 2006-02-02 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
EP07101292A EP1897939B1 (de) 2006-02-02 2007-01-29 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP07101292 Previously-Filed-Application 2007-01-29
EP07101292.6 Division 2007-01-29

Publications (2)

Publication Number Publication Date
EP2298912A2 EP2298912A2 (de) 2011-03-23
EP2298912A3 true EP2298912A3 (de) 2011-09-28

Family

ID=38282098

Family Applications (3)

Application Number Title Priority Date Filing Date
EP10175512.2A Active EP2418276B1 (de) 2006-02-02 2007-01-29 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
EP07101292A Active EP1897939B1 (de) 2006-02-02 2007-01-29 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
EP10175511A Ceased EP2298912A3 (de) 2006-02-02 2007-01-29 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen

Family Applications Before (2)

Application Number Title Priority Date Filing Date
EP10175512.2A Active EP2418276B1 (de) 2006-02-02 2007-01-29 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
EP07101292A Active EP1897939B1 (de) 2006-02-02 2007-01-29 Mikroorganismenstamm zur Produktion von rekombinanten Proteinen

Country Status (6)

Country Link
US (1) US7749756B2 (de)
EP (3) EP2418276B1 (de)
JP (1) JP4751347B2 (de)
AT (1) ATE486935T1 (de)
DE (2) DE102006004871A1 (de)
DK (2) DK1897939T3 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8060609B2 (en) 2008-01-04 2011-11-15 Sling Media Inc. Systems and methods for determining attributes of media items accessed via a personal media broadcaster
US8327407B2 (en) 2009-10-27 2012-12-04 Sling Media, Inc. Determination of receiving live versus time-shifted media content at a communication device
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040120962A1 (en) * 2002-04-15 2004-06-24 Roy Curtiss Modulation of immune responses to foreign antigens expressed by recombinant attenuated bacterial vectors

Family Cites Families (18)

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DE3813107A1 (de) * 1988-04-19 1989-11-02 Consortium Elektrochem Ind Sekretormutante von escherichia coli
DE4009268A1 (de) 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
US5264365A (en) * 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US7018809B1 (en) * 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
SE9203753D0 (sv) 1992-12-11 1992-12-11 Kabi Pharmacia Ab Expression system for producing apolipoprotein ai-m
JP3090853B2 (ja) 1994-08-19 2000-09-25 新日本製鐵株式会社 バンプ付き半導体装置の製造方法
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5830692A (en) * 1995-03-24 1998-11-03 Consortium Fur Elektrochemische Industrie Gmbh Expression system which can be regulated
GB9828711D0 (en) * 1998-12-24 1999-02-17 Genencor Int Production of proteins in gram-positive microorganisms
CA2459133A1 (en) * 2001-09-28 2003-04-10 Selectx Pharmaceuticals, Inc. Methods for generating, selecting, and identifying compounds which bind a target molecule
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
EP3023501A1 (de) 2002-03-29 2016-05-25 Danisco US Inc. Erhöhte expression von subtilisinen in bacillus
GB0224082D0 (en) * 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
KR100570422B1 (ko) * 2003-10-16 2006-04-11 한미약품 주식회사 대장균 분비서열을 이용하여 항체 단편을 분비·생산하는 발현벡터 및 이를 이용하여 항체 단편을 대량 생산하는 방법
US20050106703A1 (en) 2003-11-05 2005-05-19 Kyowa Hakko Kogyo Co., Ltd. Microorganisms producing dipeptides and process for producing dipeptides using the microorganisms
KR100656529B1 (ko) * 2004-04-08 2006-12-13 한국원자력연구소 재조합 대장균 및 상기 대장균을 이용한 폴리하이드록시부티레이트의 대량 생산방법
EP1751301A2 (de) * 2004-05-07 2007-02-14 The Regents of the University of California, Office of the President Mittels fibrinogenem gammaketten-c-terminal-fragment induzierte endothelialzellapoptose
ATE465241T1 (de) 2006-09-22 2010-05-15 Wacker Chemie Ag Verfahren zur fermentativen herstellung von proteinen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040120962A1 (en) * 2002-04-15 2004-06-24 Roy Curtiss Modulation of immune responses to foreign antigens expressed by recombinant attenuated bacterial vectors

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANDERSEN-NISSEN ERICA ET AL: "Evasion of Toll-like receptor 5 by flagellated bacteria", June 2005, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, VOL. 102, NR. 26, PAGE(S) 9247-9252, ISSN: 0027-8424, XP002646462 *
GIRON JORGE A ET AL: "The flagella of enteropathogenic Escherichia coli mediate adherence to epithelial cells", April 2002, MOLECULAR MICROBIOLOGY, VOL. 44, NR. 2, PAGE(S) 361-379, ISSN: 0950-382X, XP002646460 *
INOUE Y H ET AL: "Sequence analysis of operator mutants of the phase-1 flagellin-encoding gene, flic, in salmonella typhimurium", GENE, ELSEVIER, AMSTERDAM, NL, vol. 85, no. 1, 21 December 1989 (1989-12-21), pages 221 - 226, XP023544026, ISSN: 0378-1119, [retrieved on 19891221], DOI: 10.1016/0378-1119(89)90485-X *
WESTERLUND-WIKSTROM B ET AL: "FUNCTIONAL EXPRESSION OF ADHESIVE PEPTIDES AS FUSIONS TO ESCHERICHIA COLI FLAGELLIN", PROTEIN ENGINEERING, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 10, no. 11, 1 November 1997 (1997-11-01), pages 1319 - 1326, XP008045508, ISSN: 0269-2139, DOI: 10.1093/PROTEIN/10.11.1319 *
ZHOU XIN ET AL: "Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells.", April 2003, INFECTION AND IMMUNITY, VOL. 71, NR. 4, PAGE(S) 2120-2129, ISSN: 0019-9567, XP002646461 *

Also Published As

Publication number Publication date
EP2418276A1 (de) 2012-02-15
EP1897939B1 (de) 2010-11-03
DE102006004871A1 (de) 2007-08-09
EP1897939A2 (de) 2008-03-12
EP2418276B1 (de) 2013-11-20
EP2298912A2 (de) 2011-03-23
JP4751347B2 (ja) 2011-08-17
DE502007005530D1 (de) 2010-12-16
DK2418276T3 (en) 2014-02-24
EP1897939A3 (de) 2008-03-19
ATE486935T1 (de) 2010-11-15
US20080064062A1 (en) 2008-03-13
DK1897939T3 (da) 2011-01-31
US7749756B2 (en) 2010-07-06
JP2007202559A (ja) 2007-08-16

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