WO2004074491A3 - Expression in plants of antibodies against enterotoxigenic escherichia coli - Google Patents

Expression in plants of antibodies against enterotoxigenic escherichia coli Download PDF

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Publication number
WO2004074491A3
WO2004074491A3 PCT/EP2004/001427 EP2004001427W WO2004074491A3 WO 2004074491 A3 WO2004074491 A3 WO 2004074491A3 EP 2004001427 W EP2004001427 W EP 2004001427W WO 2004074491 A3 WO2004074491 A3 WO 2004074491A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibodies
fragments
expression
plants
escherichia coli
Prior art date
Application number
PCT/EP2004/001427
Other languages
French (fr)
Other versions
WO2004074491A2 (en
Inventor
Devon Brown
Manuel Campos
Bipin Dalmia
Stephen Demarest
Genevieve Hansen
Peter Bernard Heifetz
Original Assignee
Syngeta Participations Ag
Devon Brown
Manuel Campos
Bipin Dalmia
Stephen Demarest
Genevieve Hansen
Peter Bernard Heifetz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngeta Participations Ag, Devon Brown, Manuel Campos, Bipin Dalmia, Stephen Demarest, Genevieve Hansen, Peter Bernard Heifetz filed Critical Syngeta Participations Ag
Priority to CA002516286A priority Critical patent/CA2516286A1/en
Priority to EP04711367A priority patent/EP1597372A2/en
Priority to AU2004213554A priority patent/AU2004213554A1/en
Publication of WO2004074491A2 publication Critical patent/WO2004074491A2/en
Publication of WO2004074491A3 publication Critical patent/WO2004074491A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C07K16/1228—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K16/1232—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia from Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to the expression of antibodies and/or fragments thereof in plants, and in particular to the expression of antibodies and/or fragments thereof that have specificity for enterotoxigenic Escherichia coli. Nucleotide and amino acid sequences of such antibodies and/or fragments are disclosed, as are methods of producing such antibodies and /or fragments, transgenic plants expressing such antibodies and/or fragments thereof, and methods of using such transgenic plants or material derived therefrom, in particular in the treatment or prevention of enteric disease. Also described are methods of enhancing the stability antibodies and fragments thereof.
PCT/EP2004/001427 2003-02-18 2004-02-16 Expression in plants of antibodies against enterotoxigenic escherichia coli WO2004074491A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002516286A CA2516286A1 (en) 2003-02-18 2004-02-16 Expression in plants of antibodies against enterotoxigenic escherichia coli
EP04711367A EP1597372A2 (en) 2003-02-18 2004-02-16 Expression in plants of antibodies against enterotoxigenic escherichia coli
AU2004213554A AU2004213554A1 (en) 2003-02-18 2004-02-16 Expression in plants of antibodies against enterotoxigenic escherichia coli

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44842903P 2003-02-18 2003-02-18
US60/448,429 2003-02-18

Publications (2)

Publication Number Publication Date
WO2004074491A2 WO2004074491A2 (en) 2004-09-02
WO2004074491A3 true WO2004074491A3 (en) 2004-11-18

Family

ID=32908588

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/001427 WO2004074491A2 (en) 2003-02-18 2004-02-16 Expression in plants of antibodies against enterotoxigenic escherichia coli

Country Status (5)

Country Link
EP (1) EP1597372A2 (en)
CN (1) CN1761754A (en)
AU (1) AU2004213554A1 (en)
CA (1) CA2516286A1 (en)
WO (1) WO2004074491A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1749839A1 (en) * 2005-07-22 2007-02-07 Novoplant GmbH Antigen binding polypeptides against F4(K88) fimbriae
CA2649709A1 (en) * 2006-04-21 2007-11-01 Amgen Inc. Immunoglobulin constant region domains with enhanced stability
WO2012011396A1 (en) * 2010-07-20 2012-01-26 国立大学法人東京大学 Transgenic rice plant capable of expressing nanoantibody
CA2883566C (en) 2012-09-03 2021-05-18 Vib Vzw Protective anti-etec antibody
CN107686518B (en) * 2016-08-05 2019-12-06 中国农业大学 Single-chain antibody of anti-Escherichia coli k88ac, and coding gene and application thereof
WO2021263105A1 (en) * 2020-06-26 2021-12-30 University Of Massachusetts Anti-etec adhesin protein antibodies and methods of use
CN112094340B (en) * 2020-07-31 2023-06-20 王跃驹 Application of plant as host in expression of novel coronavirus pneumonia neutralizing antibody B38 antibody and/or H4 antibody
WO2024047558A2 (en) * 2022-08-31 2024-03-07 Immuneel Therapeutics Private Limited Antigen-binding proteins and chimeric antigen receptors specific for domain 9 of human cd307e

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000020612A2 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000020612A2 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DEMAREST S J ET AL: "Optimization of the Antibody CH3 Domain by Residue Frequency Analysis of IgG Sequences", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 335, no. 1, 2 January 2004 (2004-01-02), pages 41 - 48, XP004476444, ISSN: 0022-2836 *
KACSKOVICS I ET AL: "The heterogeneity of bovine IgG2: VIII. The complete cDNA sequence of bovine IgG2a (A2) and an IgG1", MOLECULAR IMMUNOLOGY, ELMSFORD, NY, US, vol. 33, no. 2, 1996, pages 189 - 195, XP002273988, ISSN: 0161-5890 *
SYMONS D B A ET AL: "STRUCTURE OF BOVINE IMMUNOGLOBULIN CONSTANT REGION HEAVY CHAIN GAMMA 1 AND GAMMA 2 GENES", MOLECULAR IMMUNOLOGY, ELMSFORD, NY, US, vol. 26, no. 9, 1989, pages 841 - 850, XP009027972, ISSN: 0161-5890 *
TEKOAH Y ET AL: "Controlled glycosylation of therapeutic antibodies in plants", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, NEW YORK, US, US, vol. 426, no. 2, 15 June 2004 (2004-06-15), pages 266 - 278, XP004509803, ISSN: 0003-9861 *
THIES M J W ET AL: "Folding and Oxidation of the Antibody Domain CH3", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 319, no. 5, 21 June 2002 (2002-06-21), pages 1267 - 1277, XP004449701, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
CA2516286A1 (en) 2004-09-02
WO2004074491A2 (en) 2004-09-02
EP1597372A2 (en) 2005-11-23
AU2004213554A2 (en) 2004-09-02
AU2004213554A1 (en) 2004-09-02
CN1761754A (en) 2006-04-19

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