EP2250162B1 - Dérivés d'amide en tant que modulateurs allostériques positifs et procédés d'utilisation associés - Google Patents

Dérivés d'amide en tant que modulateurs allostériques positifs et procédés d'utilisation associés Download PDF

Info

Publication number
EP2250162B1
EP2250162B1 EP09707625.1A EP09707625A EP2250162B1 EP 2250162 B1 EP2250162 B1 EP 2250162B1 EP 09707625 A EP09707625 A EP 09707625A EP 2250162 B1 EP2250162 B1 EP 2250162B1
Authority
EP
European Patent Office
Prior art keywords
indol
acetamide
pyridin
formula
trifluoromethyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP09707625.1A
Other languages
German (de)
English (en)
Other versions
EP2250162A4 (fr
EP2250162A2 (fr
Inventor
Kathleen H. Mortell
Diana L. Nersesian
Michael R. Schrimpf
Chih-Hung Lee
Murali Gopalakrishnan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AbbVie Inc
Original Assignee
AbbVie Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AbbVie Inc filed Critical AbbVie Inc
Publication of EP2250162A2 publication Critical patent/EP2250162A2/fr
Publication of EP2250162A4 publication Critical patent/EP2250162A4/fr
Application granted granted Critical
Publication of EP2250162B1 publication Critical patent/EP2250162B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/06—Antimigraine agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A61P25/34—Tobacco-abuse
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/08—Indoles; Hydrogenated indoles with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to carbon atoms of the hetero ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems

Definitions

  • the invention relates to novel amide derivatives that are positive allosteric modulators of neuronal nicotinic receptors, compositions comprising the same, and to the use of the compounds of the present invention for treating disorders or conditions.
  • Nicotinic acetylcholine receptors belong to the super family of ligand gated ion channels (LGIC), and gate the flow of cations, controlled by acetylcholine (ACh).
  • the nAChRs can be divided into nicotinic receptors of the muscular junction (NMJ) and neuronal nAChRs or neuronal nicotinic receptors (NNRs).
  • the NNRs are widely distributed throughout the central nervous system (CNS) and the peripheral nervous system (PNS).
  • the NNRs are understood to play an important role in regulating CNS function and the release of many neurotransmitters, for example, ACh, norepinephrine, dopamine, serotonin, and GABA, among others, resulting in a wide range of physiological effects.
  • nAChRs Sixteen subunits of nAChRs have been reported to date, which are identified as ⁇ 2- ⁇ 10, ⁇ 1- ⁇ 4, ⁇ , ⁇ , and ⁇ . Of these subunits, nine subunits, ⁇ 2 through ⁇ 7 and ⁇ 2 through ⁇ 4, prominently exist in the mammalian brain. Multiple functionally distinct nAChR complexes also exist, for example five ⁇ 7 subunits can form a receptor as a homomeric functional pentamer or combinations of different subunits can complex together as in the case of ⁇ 4 ⁇ 2 and ⁇ 3 ⁇ 4 receptors ( Vincler, M., et al., Exp. Opin. Ther. Targets, 2007, 11 (7): 891-897 ; Paterson, D.
  • the homomeric ⁇ 7 receptor is one of the most abundant nicotinic receptors, along with ⁇ 4 ⁇ 2 receptors, in the human brain, wherein it is heavily expressed in the hippocampus, cortex, thalamic nuclei, ventral tegmental area and substantia nigra ( Broad, L. M., et al., Drugs of the Future, 2007, 32(2): 161-170 ).
  • ⁇ 7 NNRs have been actively investigated ( Couturier, S., et al., Neuron, 1990, 5: 847-56 ).
  • the ⁇ 7 NNRs have been demonstrated to regulate interneuron excitability, modulate the release of excitatory and inhibitory neurotransmitters, and lead to neuroprotective effects in experimental in vitro models of cellular damage ( Alkondon, M., et al., Prog. Brain Res., 2004, 145: 109-20 ).
  • the NNRs in general, are involved in various cognitive functions, such as learning, memory, attention, and therefore in CNS disorders, i.e., Alzheimer's disease (AD), Parkinson's disease (PD), attention deficit hyperactivity disorder (ADHD), Tourette's syndrome, schizophrenia, bipolar disorder, pain, and tobacco dependence ( Keller, J.J., et al., Behav. Brain Res., 2005, 162: 143-52 ; Gundish, D., Expert Opin. Ther. Patents, 2005, 15 (9): 1221-1239 ; De Luca, V., et al., Acta Psychiatr. Scand., 2006, 114: 211-5 ).
  • AD Alzheimer's disease
  • PD Parkinson's disease
  • ADHD attention deficit hyperactivity disorder
  • Tourette's syndrome schizophrenia, bipolar disorder, pain, and tobacco dependence
  • the ⁇ 7 NNRs have been linked to conditions and disorders related to attention deficit disorder, ADHD, AD, mild cognitive impairment (MCI), senile dementia, dementia associated with Lewy bodies, dementia associated with Down's syndrome, AIDS dementia, Pick's disease, as well as cognitive deficits associated with schizophrenia ( Martin, L.F., et al., Psychopharmacology (Berl), 2004, 174: 54-64 ; Romanelli, M. N., et al., Exp. Opin Ther. Patents, 2007, 17(11): 1365-1377 ).
  • the ⁇ 7 NNRs have also been reported to slow disease progression in AD ( D'Andrea, M.R., et al., Curr. Pharm. Des., 2006, 12: 677-84 ).
  • modulating the activity of ⁇ 7 NNRs demonstrates promising potential to prevent or treat a variety of diseases indicated above, such as AD, other dementias, schizophrenia and neurodegeneration, with an underlying pathology that involves cognitive function including, for example, aspects of learning, memory, and attention ( Gotti, C., et al., Curr. Pharm. Des., 2006, 12: 407-428 ).
  • NNR ligands have been also implicated in smoking cessation, weight control and as potential analgesics ( Balbani, A. P. S., et al., Exp. Opin. Ther. Patents, 2003, 13 (7): 287-297 ; Gurwitz, D., Exp. Opin. Invest. Drugs, 1999, 8(6): 747-760 ; Vincler, M., Exp. Opin. Invest. Drugs, 2005, 14 (10): 1191-1198 ; Bunnelle, W. H., et al., Exp. Opin. Ther. Patents, 2003, 13 (7): 1003-1021 ; Decker, M. W., et al., Exp. Opin. Invest.
  • ⁇ 7 NNRs in the spinal cord have been identified that modulates neurotransmission associated with the pain-relieving effects of nicotinic compounds ( Cordero-Erausquin, M. et al., Proc. Nat. Acad Sci. USA 98:2803-2807, 2001 ).
  • a selective ⁇ 7 NNR partial agonist has been reported to show efficacy in models of neuropathic and persistent inflammatory pain ( Feuerbach, D. et al., Neuropharmacology, 56:254-263, 2009 ).
  • Nicotine is known to provide enhanced attention and cognitive performance, reduced anxiety, enhanced sensory gating, and analgesia and neuroprotective effects when administered. Such effects are mediated by the non-selective effect of nicotine at a variety of nicotinic receptor subtypes.
  • nicotine also produces adverse consequences, such as cardiovascular and gastrointestinal problems. Accordingly, there is a need to identify subtype-selective compounds that embrace the beneficial effects of nicotine, or an NNR ligand, while eliminating or decreasing adverse effects.
  • NNR ligands such as PNU-282987 and SSR180711A
  • PNU-282987 and SSR180711A are ⁇ 7 NNR agonists ( Hajos, M., et al., J. Pharmacol. Exp. Ther, 2005, 312: 1213-22 ; Pichat, P., et al., Society for Neuroscience Abstract, 2004, number 583.3 ).
  • AR-R17779 Another compound, AR-R17779, has been reported to improve performance of rats in social recognition, water mazes, or inhibitory avoidance models of cognitive domains ( Van Kampen, M., et al., Psychopharmacology (Berl), 2004, 172: 375-83 ). AR-R17779 also reportedly facilitates the induction of hippocampal long term potentiation (LTP) in a proposed cellular model for learning and memory in rats ( Hunter, B.E., et al., Neurosci. Lett., 1994, 168: 130-4 ). Compound A-582941, an ⁇ 7 NNR agonist, has been shown to enhance cognitive performance associated with neurodegenerative diseases such as AD and schizophrenia ( Bitner, R. S., et al., J. Neuroscience, 2007, 27(39): 10578-10587 ).
  • NNR ligands Despite the beneficial effects of NNR ligands, it remains uncertain whether chronic treatment with agonists affecting NNRs may provide suboptimal benefit due to sustained activation and desensitization of the NNR.
  • administering a positive allosteric modulator (PAM) can reinforce endogenous cholinergic transmission without directly simulating the target receptor (see for example, Albuquerque, E.X., et al., Alzheimer Dis. Assoc. Disord. 2001, 15 Suppl 1: S 19-25 ). Nicotinic PAMs could selectively modulate the activity of ACh at ⁇ 7 NNRs. Accordingly, more recently, ⁇ 7 NNR-selective PAMs have emerged ( Faghih, R., et al., Recent Patents on CNS Drug Discovery, 2007, 2 (2): 99-106 ).
  • ⁇ 7 NNR function by enhancing effects of the endogenous neurotransmitter acetylcholine via PAMs that can reinforce the endogenous cholinergic neurotransmission without directly activating ⁇ 7 NNRs, like agonists.
  • PAMs for enhancing channel activity have been proven clinically successful for GABA A receptors where benzodiazepines, barbiturates, and neurosteroids behave as PAMs acting at distinct sites ( Hevers, W., et al., Mol. Neurobiol., 1998, 18: 35-86 ).
  • NNR PAMs such as 5-hydroxyindole (5-HI), ivermectin, galantamine, bovine serum albumin, and SLURP-1, a peptide derived from acetylcholinesterase (AChE).
  • 5-HI 5-hydroxyindole
  • ivermectin a kinase inhibitor
  • galantamine galantamine
  • bovine serum albumin a peptide derived from acetylcholinesterase
  • SLURP-1 a peptide derived from acetylcholinesterase
  • genistein a kinase inhibitor was reported to increase ⁇ 7 responses
  • PNU-120596 a urea derivative
  • NNR PAMs include derivatives of quinuclidine, indole, benzopyrazole, thiazole, and benzoisothiazoles ( Hurst, R.S., et al., J. Neurosci., 2005, 25: 4396-4405 ; Broad, L. M., et al., Drugs of the Future, 2007, 32(2):161-170 ; US 7,160,876 ).
  • NNR PAMs presently known generally demonstrate weak activity, have a range of non-specific effects, or can only achieve limited access to the central nervous system where ⁇ 7 NNRs are abundantly expressed.
  • the present invention discloses novel amide derivatives that show ⁇ 7 NNR PAM activity.
  • the present invention relates to a compound of formula (III): wherein
  • the present invention relates to a pharmaceutical composition
  • a pharmaceutical composition comprising a therapeutically effective amount of the compound of formula (III) as described above in combination with a pharmaceutically acceptable carrier.
  • the present invention relates to a compound of formula (III) as described above for use in treating a disorder or condition selected from the group consisting of attention deficit disorder, attention deficit hyperactivity disorder, Alzheimer's disease, mild cognitive impairment, senile dementia, AIDS dementia, Pick's Disease, dementia associated with Lewy bodies, dementia associated with Down's syndrome, amyotrophic lateral sclerosis, Huntington's disease, diminished CNS function associated with traumatic brain injury, schizophrenia, cognitive impairment associated with schizophrenia, depression, pain, acute pain, chronic pain, post-surgical pain, inflammation, inflammatory pain, neuropathic pain, osteoarthritic pain, chronic lower back pain, migraine and smoking cessation, in a patient in need of such treatment, by administering a therapeutically effective amount of said compound of formula (III).
  • a disorder or condition selected from the group consisting of attention deficit disorder, attention deficit hyperactivity disorder, Alzheimer's disease, mild cognitive impairment, senile dementia, AIDS dementia, Pick's Disease, dementia associated with Lewy bodies, dementia associated with Down's syndrome
  • the present invention relates to a compound of formula (III) as described above for use in assessing or diagnosing conditions or disorders related to ⁇ 7 NNR activity by allowing isotope-labeled forms of the compound of formula (III) as described above to interact with cells expressing endogenous ⁇ 7 NNRs or cells expressing recombinant ⁇ 7 NNRs and measuring the effects of such isotope-labeled forms of compounds on said cells.
  • the present invention relates to a compound of formula (III) as described above for use in identifying an ⁇ 7 NNR agonist by allowing the compound of formula (III) as described above to interact with cells or cell lines endogenously expressing ⁇ 7 NNRs or cells expressing recombinant ⁇ 7 NNRs in a fluorescent medium and measuring changes in such fluorescence.
  • acetyl means a -C(O)CH 3 group.
  • alkoxy refers to an alkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom.
  • Representative examples of alkoxy include, but are not limited to, methoxy, ethoxy, propoxy, 2-propoxy, butoxy, tert-butoxy, pentyloxy, hexyloxy and the like.
  • alkyl means a straight or branched chain hydrocarbon containing from to 6 carbon atoms.
  • Representative examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, iso-propyl, n-butyl, sec-butyl, iso-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, and n-hexyl.
  • alkylcarbonyl refers to an alkyl group, as defined herein, appended to the parent molecular moiety through a carbonyl group, as defined herein.
  • Representative examples of alkylcarbonyl include, but are not limited to, acetyl, 1-oxopropyl, 2,2-dimethyl-1-oxopropyl, 1-oxobutyl, 1-oxopentyl, and the like.
  • amino refers to -NR 90 R 91 , wherein R 90 and R 91 are independently selected from hydrogen and alkyl, as defined herein.
  • Representative examples of amino include, but are not limited to, amino, methylamino, ethylmethylamino, methylisopropylamino, dimethylamino, diisopropylamino, diethylamino, and the like.
  • aryl means phenyl, a bicyclic aryl or a tricyclic aryl.
  • the bicyclic aryl is naphthyl, a phenyl fused to a cycloalkyl, or a phenyl fused to a cycloalkenyl.
  • Representative examples of the bicyclic aryl include, but are not limited to, dihydroindenyl, indenyl, naphthyl, dihydronaphthalenyl, and tetrahydronaphthalenyl.
  • the tricyclic aryl is anthracene or phenanthrene, or a bicyclic aryl fused to a cycloalkyl, or a bicyclic aryl fused to a cycloalkenyl, or a bicyclic aryl fused to a phenyl.
  • Representative examples of tricyclic aryl ring include, but are not limited to, azulenyl, dihydroanthracenyl, fluorenyl, and tetrahydrophenanthrenyl.
  • aryl groups of this invention can be substituted with 0, 1, 2, or 3 substituents independently selected from alkoxy, alkyl, aryloxy, carboxy, carboxyalkyl, cycloalkyl, cycloalkoxy, halogen, haloalkoxy, haloalkyl, halothioalkoxy, hydroxyl, mercapto, thioalkoxy, thiocycloalkoxy, and thioaryloxy.
  • aryloxy means an aryl group, as defined herein, appended to the parent molecular moiety through an oxygen atom.
  • Representative examples of aryloxy include, but are not limited to, phenoxy and tolyloxy.
  • carbonyl means a -C(O)- group.
  • carboxy means a -CO 2 H group.
  • carboxyalkyl means a carboxy group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
  • Representative examples of carboxyalkyl include, but are not limited to, carboxymethyl, 2-carboxyethyl, and 3-carboxypropyl.
  • cycloalkoxy means a cycloalkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom.
  • Representative examples of cycloalkoxy include, but are not limited to, cyclohexyloxy and cyclopropoxy.
  • cycloalkyl refers to a monocyclic ring system.
  • Monocyclic ring systems are exemplified by a saturated cyclic hydrocarbon group containing from 3 to 6 carbon atoms.
  • Representative examples of monocyclic ring systems include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like.
  • halo or halogen means -Cl, -Br, -I or -F.
  • haloalkoxy means at least one halogen, as defined herein, appended to the parent molecular moiety through an alkoxy group, as defined herein.
  • Representative examples of haloalkoxy include, but are not limited to, 2-fluoroethoxy, trifluoromethoxy, and pentafluoroethoxy.
  • haloalkyl means at least one halogen, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
  • Representative examples of haloalkyl include, but are not limited to, difluoromethyl, chloromethyl, 2-fluoroethyl, trifluoromethyl, 2,2,2-trifluoroethyl, pentafluoroethyl, and 2-chloro-3-fluoropentyl.
  • halothioalkoxy means at least one halogen, as defined herein, appended to the parent molecular moiety through a thioalkoxy group, as defined herein.
  • Representative examples of halothioalkoxy include, but are not limited to, 2-chloroethylsulfane and trifluoromethylsulfane.
  • heteroaryl means a monocyclic heteroaryl or a bicyclic heteroaryl.
  • the monocyclic heteroaryl is a 5 or 6 membered ring that contains at least one heteroatom selected from the group consisting of nitrogen, oxygen and sulfur.
  • the 5 membered ring contains two double bonds and the 6 membered ring contains three double bonds.
  • the 5 or 6 membered heteroaryl is connected to the parent molecular moiety through any carbon atom or any substitutable nitrogen atom contained within the heteroaryl, provided that proper valance is maintained.
  • monocyclic heteroaryl include, but are not limited to, furyl, imidazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, oxazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, pyrazolyl, pyrrolyl, tetrazolyl, thiadiazolyl, thiazolyl, thienyl, triazolyl, and triazinyl.
  • the bicyclic heteroaryl consists of a monocyclic heteroaryl fused to a phenyl, or a monocyclic heteroaryl fused to a cycloalkyl, or a monocyclic heteroaryl fused to a cycloalkenyl, or a monocyclic heteroaryl fused to a monocyclic heteroaryl.
  • the bicyclic heteroaryl is connected to the parent molecular moiety through any carbon atom or any substitutable nitrogen atom contained within the bicyclic heteroaryl, provided that proper valance is maintained.
  • bicyclic heteroaryl include, but are not limited to, azaindolyl, benzimidazolyl, benzofuranyl, benzoxadiazolyl, benzoisoxazole, benzoisothiazole, benzooxazole, 1,3-benzothiazolyl, benzothiophenyl, cinnolinyl, furopyridine, indolyl, indazolyl, isobenzofuran, isoindolyl, isoquinolinyl, naphthyridinyl, oxazolopyridine, 1H-pyrrolo[2,3-b]pyridinyl, quinolinyl, quinoxalinyl and thienopyridinyl.
  • heteroaryl groups of the invention are optionally substituted with 0, 1, 2, 3 or 4 substituents independently selected from the group consisting of alkoxy, alkyl, aryl, aryloxy, carboxy, carboxyalkyl, cycloalkyl, cycloalkoxy, haloalkoxy, haloalkyl, halogen, halothioalkoxy, thioalkoxy, thiocycloalkoxy, thioaryloxy.
  • hydroxy or "hydroxyl” means an -OH group.
  • mercapto means a -SH group.
  • thioalkoxy refers to an alkyl group, as defined herein, appended to the parent molecular moiety through a sulfur atom.
  • Representative examples of thioalkoxy include, but are not limited to, Representative examples of thioalkoxy include, but are not limited, methylthio, ethylthio, tert-butylthio, and hexylthio.
  • thiocyloalkoxy refers to an cycloalkyl group, as defined herein, appended to the parent molecular moiety through a sulfur atom.
  • Representative examples of thiocycloalkoxy include, but are not limited to, cyclopentylsulfane and cyclohexylsulfane.
  • thiaryloxy means an aryl group, as defined herein, appended to the parent molecular moiety through a sulfur atom.
  • Representative examples of thioaryloxy include, but are not limited to, thiophenoxy and tolylsulfane.
  • parenterally refers to modes of administration, including intravenous, intramuscular, intraperitoneal, intrasternal, subcutaneous, intraarticular injection and infusion.
  • PAM Positive Allosteric Modulator
  • salt refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, and are commensurate with a reasonable benefit/risk ratio.
  • Pharmaceutically acceptable salts are well-known in the art. The salts can be prepared in situ during the final isolation and purification of the compounds of the invention or separately by reacting a free base function with a suitable organic acid.
  • Representative acid addition salts include, but are not limited to acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate, digluconate, glycerophosphate, hemisulfate, heptanoate, hexanoate, fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethansulfonate (isethionate), lactate, maleate, methanesulfonate, nicotinate, 2-naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, phosphate, glutamate, bicarbonate, p-toluenesulfonate and undecanoate.
  • the basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides; dialkyl sulfates such as dimethyl, diethyl, dibutyl and diamyl sulfates; long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides; arylalkyl halides such as benzyl and phenethyl bromides and others. Water or oil-soluble or dispersible products are thereby obtained.
  • lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides
  • dialkyl sulfates such as dimethyl, diethyl, dibutyl and diamyl sulfates
  • long chain halides such as de
  • acids which can be employed to form pharmaceutically acceptable acid addition salts include such inorganic acids as hydrochloric acid, hydrobromic acid, sulfuric acid and phosphoric acid and such organic acids as oxalic acid, maleic acid, succinic acid, and citric acid.
  • Basic addition salts can be prepared in situ during the final isolation and purification of compounds of this invention by reacting a carboxylic acid-containing moiety with a suitable base such as the hydroxide, carbonate or bicarbonate of a pharmaceutically acceptable metal cation or with ammonia or an organic primary, secondary or tertiary amine.
  • Pharmaceutically acceptable salts include, but are not limited to, cations based on alkali metals or alkaline earth metals such as lithium, sodium, potassium, calcium, magnesium, and aluminum salts, and the like, and nontoxic quaternary ammonia and amine cations including ammonium, tetramethylammonium, tetraethylammonium, methylammonium, dimethylammonium, trimethylammonium, triethylammonium, diethylammonium, ethylammonium and the like.
  • Other representative organic amines useful for the formation of base addition salts include ethylenediammonium, ethanolammonium, diethanolammonium, piperidinium, and piperazinium.
  • pharmaceutically acceptable carrier means a non-toxic, inert solid, semi-solid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type.
  • materials which can serve as pharmaceutically acceptable carriers are sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols such a propylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isot
  • ⁇ 7 includes homomeric ( ⁇ 7) 5 receptors and ⁇ 7* receptors, which denote an NNR containing at least one ⁇ 7 subunit.
  • One embodiment relates to compounds of formula (I): or a pharmaceutically acceptable salt, ester, amide or prodrug thereof, wherein
  • Another embodiment relates to compounds of formula (II): or a pharmaceutically acceptable salt, ester, amide or prodrug thereof, wherein
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein a is a double bond and X 2 is CH.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein a is a single bond and X 2 is CH 2 or -CH 2 CH 2 -.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein X 1 is CH.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein X 1 is N.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein X 3 and X 4 are both CH.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein X 3 and X 4 are both N.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein one of X 3 and X 4 is N and the other is CH.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein at least one of T 1 and T 2 is aryl, cycloalkyl, halo, or haloalkyl.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein at least one of T 1 and T 2 is R 3 -L 2 -, wherein L 2 is O or S and R 3 is alkyl, haloalkyl or aryl.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein R 1 and R 2 are both hydrogen, and a is a double bond.
  • Another embodiment is a compound of formula (II) or a pharmaceutically acceptable salt, ester, amide or prodrug thereof as described above, wherein one of R 1 or R 2 is hydrogen, the other is methyl, and a is a double bond.
  • the present invention relates to compounds of formula (III): or a pharmaceutically acceptable salt thereof, wherein
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein a is a double bond and X 2 is CH.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein a is a single bond and X 2 is CH 2 or -CH 2 CH 2 -.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein X 1 is CH.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein X 1 is N.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein X 3 and X 4 are both CH.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable shalt thereof as described above, wherein X 3 and X 4 are both N.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein one of X 3 and X 4 is N and the other is CH.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein at least one of T 1 and T 2 is aryl, cycloalkyl, halo, or haloalkyl.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein at least one of T 1 and T 2 is R 3 -L 2 -, wherein L 2 is O or S and R 3 is alkyl, haloalkyl or aryl.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein R 1 and R 2 are both hydrogen, and a is a double bond.
  • Another embodiment is a compound of formula (III) or a pharmaceutically acceptable salt thereof as described above, wherein one of R 1 or R 2 is hydrogen, the other is methyl, and a is a double bond.
  • Stereoisomers include enantiomers and diastereomers, and mixtures of enantiomers or diastereomers.
  • Individual stereoisomers of compounds of the invention may be prepared synthetically from commercially available starting materials which contain asymmetric or chiral centers or by preparation of racemic mixtures followed by resolution well-known to those of ordinary skill in the art. These methods of resolution are exemplified by (1) attachment of a mixture of enantiomers to a chiral auxiliary, separation of the resulting mixture of diastereomers by recrystallization or chromatography and optional liberation of the optically pure product from the auxiliary as described in Furniss, Hannaford, Smith, and Tatchell, "Vogel's Textbook of Practical Organic Chemistry", 5th edition (1989), Longman Scientific & Technical, Essex CM20 2JE, Engl and, or (2) direct separation of the mixture of optical enantiomers on chiral chromatographic columns or (3) fractional recrystallization methods.
  • Esters can be prepared from substrates of formula (I), formula (II), or formula (III) containing either a hydroxyl group or a carboxy group by general methods known to persons skilled in the art.
  • the typical reactions of these compounds are substitutions replacing one of the heteroatoms by another atom, for example:
  • Amides can be prepared from substrates of formula (I), formula (II), or formula (III) containing either an amino group or a carboxy group in similar fashion. Esters can also react with amines or ammonia to form amides.
  • Another way to make amides from compounds of formula (I), formula (II), or formula (III) is to heat carboxylic acids and amines together.
  • R and R' are independently substrates of formula (I), formula (II), formula (III), alkyl or hydrogen.
  • Various embodiments of formula (I), formula (II), or formula (III) that are substrates for prodrugs, amides and esters include, but not limited to, Examples 1, 2, 3, 4, 5, 6, 7, 8, 14, 15, 16, 17, 19, 22, 23 and Examples 26-197.
  • Examples 24 and 25 are representative prodrugs of the invention.
  • the invention also provides pharmaceutical compositions comprising a therapeutically effective amount of a compound of formula (III) in combination with a pharmaceutically acceptable carrier.
  • the compositions comprise compounds of the invention formulated together with one or more non-toxic pharmaceutically acceptable carriers.
  • the pharmaceutical compositions can be formulated for oral administration in solid or liquid form, for parenteral injection or for rectal administration.
  • compositions of this invention can be administered to humans and other mammals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments or drops), bucally or as an oral or nasal spray.
  • compositions for parenteral injection comprise pharmaceutically acceptable sterile aqueous or nonaqueous solutions, dispersions, suspensions or emulsions and sterile powders for reconstitution into sterile injectable solutions or dispersions.
  • suitable aqueous and nonaqueous carriers, diluents, solvents or vehicles include water, ethanol, polyols (propylene glycol, polyethylene glycol, glycerol, and the like, and suitable mixtures thereof), vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate, or suitable mixtures thereof.
  • Suitable fluidity of the composition may be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.
  • compositions can also contain adjuvants such as preservative agents, wetting agents, emulsifying agents, and dispersing agents.
  • adjuvants such as preservative agents, wetting agents, emulsifying agents, and dispersing agents.
  • Prevention of the action of microorganisms can be ensured by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and the like. It also can be desirable to include isotonic agents, for example, sugars, sodium chloride and the like.
  • Prolonged absorption of the injectable pharmaceutical form can be brought about by the use of agents delaying absorption, for example, aluminum monostearate and gelatin.
  • a parenterally administered drug form can be administered by dissolving or suspending the drug in an oil vehicle.
  • Suspensions in addition to the active compounds, can contain suspending agents, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, tragacanth, and mixtures thereof.
  • suspending agents for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, tragacanth, and mixtures thereof.
  • the compounds of the invention can be incorporated into slow-release or targeted-delivery systems such as polymer matrices, liposomes, and microspheres. They may be sterilized, for example, by filtration through a bacteria-retaining filter or by incorporation of sterilizing agents in the form of sterile solid compositions, which may be dissolved in sterile water or some other sterile injectable medium immediately before use.
  • Injectable depot forms are made by forming microencapsulated matrices of the drug in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of drug to polymer and the nature of the particular polymer employed, the rate of drug release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations also are prepared by entrapping the drug in liposomes or microemulsions which are compatible with body tissues.
  • the injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium just prior to use.
  • sterile injectable aqueous or oleaginous suspensions can be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation also can be a sterile injectable solution, suspension or emulsion in a nontoxic, parenterally acceptable diluent or solvent such as a solution in 1,3-butanediol.
  • acceptable vehicles and solvents that can be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil can be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid are used in the preparation of injectables.
  • Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
  • one or more compounds of the invention is mixed with at least one inert pharmaceutically acceptable carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and salicylic acid; b) binders such as carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia; c) humectants such as glycerol; d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate; e) solution retarding agents such as paraffin; f) absorption accelerators such as quaternary ammonium compounds; g) wetting agents such as cetyl alcohol and glycerol monostearate; h
  • compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using lactose or milk sugar as well as high molecular weight polyethylene glycols.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well-known in the pharmaceutical formulating art. They can optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract in a delayed manner. Examples of materials useful for delaying release of the active agent can include polymeric substances and waxes.
  • compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • suitable non-irritating carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
  • the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and
  • the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
  • adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
  • Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
  • a desired compound of the invention is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
  • Ophthalmic formulation, eardrops, eye ointments, powders and solutions are also contemplated as being within the scope of this invention.
  • the ointments, pastes, creams and gels may contain, in addition to an active compound of this invention, animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.
  • Powders and sprays can contain, in addition to the compounds of this invention, lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances.
  • Sprays can additionally contain customary propellants such as chlorofluorohydrocarbons.
  • liposomes are generally derived from phospholipids or other lipid substances. Liposomes are formed by mono- or multi-lamellar hydrated liquid crystals that are dispersed in an aqueous medium. Any non-toxic, physiologically acceptable and metabolizable lipid capable of forming liposomes may be used.
  • the present compositions in liposome form may contain, in addition to the compounds of the invention, stabilizers, preservatives, and the like.
  • the preferred lipids are the natural and synthetic phospholipids and phosphatidylcholines (lecithins) used separately or together.
  • Dosage forms for topical administration of a compound of this invention include powders, sprays, ointments and inhalants.
  • the active compound is mixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives, buffers or propellants.
  • Ophthalmic formulations, eye ointments, powders and solutions are also contemplated as being within the scope of this invention.
  • Aqueous liquid compositions of the invention also are particularly useful.
  • the compounds and compositions of the invention can be used in the form of pharmaceutically acceptable salts, esters, or amides derived from inorganic or organic acids.
  • the pharmaceutically acceptable salts, esters and amides include salts, zwitterions, esters and amides of compounds of formula (I), formula (II), or formula (III) which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, are commensurate with a reasonable benefit/risk ratio, and are effective for their intended use.
  • Radiolabelled or isotopically labelled forms of compounds of formula (I), formula (II), or formula (III) are provided as compositions of the invention and administered in accordance with the method of the invention.
  • the radiolabelled or isotopically labelled forms of compounds of formula (I), formula (II), or formula (III) may be used as a pharmaceutical agent or may be useful in the discovery of other compounds which are modulators of ⁇ 7 NNR.
  • the compounds of the invention possess at least one atom of a deuterium or tritium.
  • Compounds and compositions of the invention are useful for modulating the effects of NNRs, particularly by allosteric modulation. Such compounds can be useful for the treatment and prevention of a number of NNR-mediated diseases or conditions.
  • a population of ⁇ 7 NNRs in the spinal cord modulate serotonergic transmission that has been associated with the pain-relieving effects of nicotinic compounds ( Cordero-Erausquin, M., et al., Proc. Nat. Acad. Sci., 2001, 98: 2803-2807 ).
  • the ⁇ 7 NNR ligands demonstrate therapeutic potential for the treatment of pain states, including acute pain, post-surgical pain, as well as chronic pain states including inflammatory pain and neuropathic pain.
  • ⁇ 7 NNRs are expressed on the surface of primary macrophages that are involved in the inflammation response, and that activation of the ⁇ 7 NNR inhibits release of tumor necrosis factor (TNF) and other cytokines that trigger the inflammation response ( Wang, H., Nature, 2003, 421: 384-388 ).
  • TNF- ⁇ plays a pathological role in diverse inflammatory diseases including arthritis and psoriasis and endometriosis. Therefore, selective ⁇ 7 NNR ligands and modulators demonstrate potential for treating conditions involving inflammation and pain.
  • ⁇ 7 NNRs have been shown to play a significant role in enhancing cognitive function, including aspects of learning, memory and attention ( Levin, E.D., J. Neurobiol., 2002, 53: 633-640 ).
  • ⁇ 7 ligands are suitable for the treatment of cognitive disorders including, for example, attention deficit disorder, ADHD, AD, MCI, senile dementia, AIDS dementia, Pick's Disease, dementia associated with Lewy bodies, and dementia associated with Down's syndrome, as well as cognitive deficits associated with schizophrenia.
  • ⁇ 7 NNRs have been shown to be involved in the neuroprotective effects of nicotine both in vitro ( Jonnala, R.B. et al., J. Neurosci. Res., 2001, 66: 565-572 ) and in vivo ( Shimohama, S., Brain Res., 1998, 779: 359-363 ). More particularly, neurodegeneration underlies several progressive CNS disorders, including, but not limited to, AD, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, dementia with Lewy bodies, as well as diminished CNS function resulting from traumatic brain injury.
  • ⁇ 7 NNRs have been implicated as a key factor in development of the cognitive deficits associated with the disease ( Liu, Q.-S., et al., PNAS, 2001, 98: 4734-4739 ).
  • the activation of ⁇ 7 NNRs has been shown to block this neurotoxicity ( Kihara, T., J. Biol. Chem., 2001, 276: 13541-13546 ).
  • selective ligands that enhance ⁇ 7 activity can counter the deficits of Alzheimer's and other neurodegenerative diseases.
  • Schizophrenia is a complex disease that is characterized by abnormalities in perception, cognition, and emotions. Significant evidence implicates the involvement of ⁇ 7 NNRs in this disease, including a measured deficit of these receptors in post-mortem patients ( Leonard, S., Eur. J. Pharmacol., 2000, 393: 237-242 ). Deficits in sensory processing (gating) are one of the hallmarks of schizophrenia. These deficits can be normalized by nicotinic ligands that operate at the ⁇ 7 NNR ( Adler, L.E., Schizophrenia Bull., 1998, 24: 189-202 ; Stevens, K.E., Psychopharmacology, 1998, 136: 320-327 ). Thus, ⁇ 7 NNR ligands demonstrate potential in the treatment of schizophrenia.
  • Compounds of the invention are particularly useful for treating and preventing a condition or disorder affecting cognition, neurodegeneration, and schizophrenia.
  • Cognitive impairment associated with schizophrenia often limits the ability of patients to function normally; a symptom not adequately treated by commonly available treatments, for example, treatment with an atypical antipsychotic ( Rowley, M., J. Med. Chem., 2001, 44: 477-501 ).
  • Such cognitive deficit has been linked to dysfunction of the nicotinic cholinergic system, in particular with decreased activity at ⁇ 7 NNR receptors ( Friedman, J.I., Biol. Psychiatry, 2002, 51: 349-357 ).
  • activators of ⁇ 7 NNR receptors can provide useful treatment for enhancing cognitive function in schizophrenic patients who are being treated with atypical antipsychotics. Accordingly, the combination of an ⁇ 7 NNR modulator and an atypical antipsychotic would offer improved therapeutic utility.
  • suitable atypical antipsychotics include, but are not limited to, clozapine, risperidone, olanzapine, quietapine, ziprasidone, zotepine, iloperidone, and the like. Accordingly, it is contemplated that compounds of formula (I), formula (II), or formula (III) of the invention also can be administered in combination with an atypical antipsychotic.
  • NNR ligands that are selective for the ⁇ 7 subtype and can be used in therapy for smoking cessation, with an improved side effect profile compared to nicotine.
  • the compound of formula (III) are useful for treating or preventing condition or disorder selected from attention deficit disorder, ADHD, AD, MCI, senile dementia, AIDS dementia, Pick's Disease, dementia associated with Lewy bodies, dementia associated with Down's syndrome, amyotrophic lateral sclerosis, Huntington's disease, diminished CNS function associated with traumatic brain injury, depression, pain, acute pain, post-surgical pain, chronic pain, inflammation, inflammatory pain, neuropathic pain, osteoarthritic pain, chronic lower back pain, migraine, and smoking cessation, by administration of a therapeutically effective amount of said compound to a mammal.
  • an embodiment is directed to the compounds of formula (III) or therapeutically effective compositions of compounds of formula (III) for use in treating conditions and disorders that are regulated by the NNR.
  • compositions containing compounds of formula. (III) can be administered in accordance with described methods, typically as part of a therapeutic regimen for treatment or prevention of conditions and disorders related to NNR activity, and more particularly allosteric modulation of NNR activity.
  • Another embodiment relates to compounds of formula (III) for use in treating or preventing conditions and disorders related to NNR modulation in mammals. More particularly, the compounds are useful for conditions and disorders related to attention deficit disorder, ADHD, AD, MCI, schizophrenia, senile dementia, AIDS dementia, Pick's Disease, dementia associated with Lewy bodies, dementia associated with Down's syndrome, amyotrophic lateral sclerosis, Huntington's disease, diminished CNS function associated with traumatic brain injury, depression, pain, acute pain, post-surgical pain, chronic pain, inflammation, inflammatory pain, neuropathic pain, osteoarthritic pain, chronic lower back pain, migraine, smoking cessation, and various other conditions modulated by ⁇ 7 NNRs modulators.
  • Another embodiment relates to compounds of formula (III) for use in treating or preventing conditions and disorders related to NNR modulation in mammals, wherein the conditions and disorders are attention deficit disorder, ADHD, AD, MCI, schizophrenia, senile dementia, AIDS dementia, Pick's Disease, dementia associated with Lewy bodies, dementia associated with Down's syndrome, amyotrophic lateral sclerosis, Huntington's disease, diminished CNS function associated with traumatic brain injury and depression.
  • Another embodiment relates to compounds of formula (III) for use in treating or preventing conditions and disorders related to NNR modulation in mammals, wherein the conditions and disorders are pain, acute pain, post-surgical pain, chronic pain, inflammation, inflammatory pain, neuropathic pain, osteoarthritic pain, chronic lower back pain, migraine, and smoking cessation.
  • Another embodiment relates to compounds of formula (III) for use in treating or preventing conditions and disorders related to NNR modulation in mammals. More particularly, the method is useful for combining a compound of formula (III) with an atypical antipsychotic. Further, in another embodiment, the invention relates to the way of administering compositions containing compounds of formula (III) in combination with a nicotinic agonist or an atypical antipsychotic.
  • compositions or compounds of formula (III), or a pharmaceutically acceptable salt thereof in combination with a cholinesterase inhibitor or another drug that increases endogenous acetylcholine release such as histamine H3 antagonists, 5HT-6 antagonists, dopamine D3 agonists, muscarinic receptor antagonists and potassium channel blockers, leading to potentiation of effects at the ⁇ 7 nicotinic receptor subtype.
  • a cholinesterase inhibitor or another drug that increases endogenous acetylcholine release such as histamine H3 antagonists, 5HT-6 antagonists, dopamine D3 agonists, muscarinic receptor antagonists and potassium channel blockers, leading to potentiation of effects at the ⁇ 7 nicotinic receptor subtype.
  • Actual dosage levels of active ingredients in the pharmaceutical compositions of this invention can be varied so as to obtain an amount of the active compound(s) that is effective to achieve the desired therapeutic response for a particular patient, compositions and mode of administration.
  • the selected dosage level will depend upon the activity of the particular compound, the route of administration, the severity of the condition being treated and the condition and prior medical history of the patient being treated. However, it is within the skill of the art to start doses of the compound at levels lower than required to achieve the desired therapeutic effect and to gradually increase the dosage until the desired effect is achieved.
  • a therapeutically effective amount of one of the compounds of the invention can be employed in pure form or, where such forms exist, in pharmaceutically acceptable salt, ester, amide or prodrug form.
  • the compound can be administered as a pharmaceutical composition containing the compound of interest in combination with one or more pharmaceutically acceptable carriers.
  • therapeutically effective amount means a sufficient amount of the compound to treat disorders, at a reasonable benefit/risk ratio applicable to any medical treatment. It will be understood, however, that the total daily usage of the compounds and compositions of the invention will be decided by the attending physician within the scope of sound medical judgment.
  • the specific therapeutically effective dose level for any particular patient will depend upon a variety of factors including the disorder being treated and the severity of the disorder; activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed; and like factors well-known in the medical arts. For example, it is well within the skill of the art to start doses of the compound at levels lower than required to achieve the desired therapeutic effect and to gradually increase the dosage until the desired effect is achieved.
  • the total daily dose of the compounds of this invention administered to a human or lower animal ranges from about 0.001 mg/kg body weight to about I g/kg body weight. More preferable doses can be in the range of from about 0.001 mg/kg body weight to about 100 mg/kg body weight. If desired, the effective daily dose can be divided into multiple doses for purposes of administration. Consequently, single dose compositions may contain such amounts or submultiples thereof to make up the daily dose.
  • Another embodiment relates to a method of assessing or diagnosing conditions or disorders related to ⁇ 7 NNR activity comprising allowing isotope-labeled forms of compounds of formula (III) to interact with cells expressing endogenous ⁇ 7 NNRs or cells expressing recombinant ⁇ 7 NNRs and measuring the effects of such isotope-labeled forms of compounds on such cells as explained in Determination of Biological Activity section.
  • Another embodiment relates to NNR PAM compounds to identify other useful target compounds for use in treating or preventing, or both, diseases or conditions associated with NNR function, in cell-based assays, for example in high-throughput format, using cells or tissues that express native ⁇ 7 NNRs for the purpose of identifying novel ⁇ 7 NNR agonists or PAMs of ⁇ 7 NNRs.
  • Another embodiment relates to a compound of formula (III) for use in identifying an ⁇ 7 NNR agonist by allowing a compound of formula (III) to interact with cells or cell lines endogenously expressing ⁇ 7 NNRs or cells expressing recombinant ⁇ 7 NNRs in a fluorescent medium and measuring changes in such fluorescence by known protocols or as described in Determination of Biological Activity section.
  • the methods described below can entail use of various enantiomers.
  • the compounds of this invention can be prepared according to the synthetic methods described in this section, Methods of the Invention and Examples sections. Certain groups described in the Schemes are meant to illustrate certain substituents contained within the invention and are not intended to limit the scope of the invention. Representative procedures are shown in, but are not limited to, Schemes 4-11. It is noted that the preparation of the compounds is included in the present invention to the extent applicable.
  • Alternative conditions and reagents to form compounds of formula 3 include combining an equimolar mixture of the compounds of formula 1 and compounds of formula 2 with a coupling reagent such as but not limited to bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BOPCI), 1,3-dicyclohexylcarbodiimide (DCC), polymer supported 1,3-dicyclohexylcarbodiimide (PS-DCC), O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), O-benzotriazol-1-yl-N,N,N',N'-tetramethyluronium tetrafluoroborate (TBTU) optionally along with a coupling auxiliary such as but not limited to 1-hydroxy-7-azabenzotriazole (HOAT) or 1-hydroxybenzotriazole hydrate (HOBT) in the presence or
  • compounds of formula 6 are representative of compounds of formula (III), wherein R 1 , R 2 , T 1 , T 2 , X 1 , X 2 , X 3 , X 4 , and a are as defined in formula (III), can be prepared accordingly,
  • Compounds of formula 6 can be prepared by combining an equimolar mixture of the compounds of formula 4 and compounds of formula 5 with a coupling reagent such as but not limited to bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BOPCl), 1,3-dicyclohexyl carbodiimide (DCC), polymer supported 1,3-dicyclohexylcarbodiimide (PS-DCC), O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), O-benzotriazol-1-yl-N,N,N',N'
  • compounds of formula 4 can be prepared from compounds of formula 1 by a sequence involving a Curtius rearrangement.
  • Methods for effecting this transformation include treatment with diphenylphosphoryl azide in a solvent such as toluene or an alcohol, with trapping of the intermediate isocyanate by water, methanol, benzyl alcohol, or allyl alcohol.
  • Compounds of formula 4 can be used as described in Scheme 5.
  • carboxylic acids of formula 1 wherein T 1 , T 2 , X 3 , and X 4 are as defined in formula (III) and M is lithium or magnesium
  • M lithium or magnesium
  • X 5 is Cl, Br, I, or trifluoromethylsulfonate
  • 8 can be treated with palladium and methyl chloroformate.
  • Compounds of formula 1 can be used as described in Scheme 4.
  • compounds of formula 11 wherein X 4 is as defined in formula (III), can be prepared from compounds of formula 10 , wherein R 4 is methyl, ethyl, or benzyl, and sodium isopropoxide in a solvent such as 2-propanol, followed by ester hydrolysis using either an aqueous acid such as hydrochloric acid or a base such as sodium hydroxide, potassium hydroxide, or lithium hydroxide in water or a mixture of water and a cosolvent such as tetrahydrofuran ( Konno, S.; et al., Heterocycles 1992, 34, 225-8 ).
  • Compounds of formula 11 can be used in place of compounds of formula 1 in Scheme 4.
  • compounds of formula 13 wherein X 3 and X 4 are as defined in formula (III), can be prepared from compounds of formula 12 , wherein X 6 is bromine, iodine, or chlorine and R 4 is methyl, ethyl, or benzyl, by reacting with either cyclopropylboronic acid or potassium cyclopropyl trifluoroborate under cross-coupling reaction conditions according to the following literature descriptions: Wallace, D. J.; et al., Tetrahedron Lett. 2002, 43, 6987-6990 ; Fang, G.-H.; Yan, Z.-J.; et al., Org. Lett. 2004, 6, 357-360 ; or Charette, A.
  • esters of formula 13 can be hydrolyzed to the corresponding carboxylic acids using methodologies known to one skilled in the art which can then be used in place of compounds of formula 1 in Scheme 4.
  • compounds of formula 16 or formula 19 wherein X 3 and X 4 are as defined for formula (III), R 4 is methyl, ethyl, or benzyl, and PG represents a nitrogen protecting group as described below, can be prepared from compounds of formula 15 and formula 18 , respectively, wherein R 6 is H or SR 5 (SR 5 is indicative of a symmetric disulfide) by treatment with trimethylsilyl trifluoromethane and a catalytic amount of an activator such as tetrabutylammonium fluoride ( Prakash, G. K. S.; et al., Chem. Rev. 1997, 97, 757-786 ).
  • an activator such as tetrabutylammonium fluoride
  • conversion to the trifluoromethylsulfide can be effected by treatment with at least 4.2 equivalents of iodotrifluoromethane and 2.2 equivalents of tetrakis(dimethylamino)ethylene (TDAE) in N,N-dimethylformamide at 0 °C to room temperature ( Carre ⁇ o, M. C.; et al., Org. Lett. 2004, 6, 297-299 ).
  • TDAE tetrakis(dimethylamino)ethylene
  • the ester of compounds of formula 16 and protecting groups of compounds of formula 19 can be removed with methodologies known to one skilled in the art.
  • the corresponding carboxylic acids of compounds of formula 16 can be used in place of compounds of formula 1 in Scheme 4.
  • the corresponding anilines of compounds of formula 19 can be used in place of compounds of formula 4 in Scheme 5.
  • the phenols or thiophenols of formula 21 and formula 23 can be coupled under Ullmann reaction conditions with the corresponding aryl halides or aryl triflates of formula 20 and formula 24 , respectively.
  • Copper-mediated Ullmann reaction conditions to prepare diaryl ethers or diaryl thioethers have been described in the following literature citation: Ley, S. V.; et al., Angew. Chem. Int. Ed. 2003, 42, 5400-5449 .
  • Compounds of formula 22 upon conversion to the corresponding carboxylic acids or anilines can be used in Schemes 4 and 5, respectively.
  • nitrogen protecting groups can be used for protecting amine groups during the synthesis of compounds of formula (III). Such methods, and some suitable nitrogen protecting groups, are described in Greene and Wuts (Protective Groups In Organic Synthesis, Wiley and Sons, 1999 ).
  • suitable nitrogen protecting groups include, but are not limited to, tert-butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), benzyl (Bn), acetyl, and trifluoracetyl. More particularly, the Boc protecting group may be removed by treatment with an acid such as trifluoroacetic acid or hydrochloric acid.
  • the Cbz and Bn protecting groups may be removed by catalytic hydrogenation and acetyl and trifluoracetyl protecting groups may be removed by variety of conditions including the use of sodium, potassium or lithium hydroxide in aqueous organic or alcoholic solvents.
  • the compounds and intermediates of the invention may be isolated and purified by methods well-known to those skilled in the art of organic synthesis.
  • Examples of conventional methods for isolating and purifying compounds can include, but are not limited to, chromatography on solid supports such as silica gel, alumina, or silica derivatized with alkylsilane groups, by recrystallization at high or low temperature with an optional pretreatment with activated carbon, thin-layer chromatography, distillation at various pressures, sublimation under vacuum, and trituration, as described for instance in " Vogel's Textbook of Practical Organic Chemistry", 5th edition (1989), by Furniss, Hannaford, Smith, and Tatchell, pub. Longman Scientific & Technical, Essex CM20 2JE, Engl and.
  • Some compounds of the invention have at least one basic site whereby the compound can be treated with an acid to form a desired salt.
  • a compound may be reacted with an acid at or above room temperature to provide the desired salt, which is deposited, and collected by filtration after cooling.
  • acids suitable for the reaction include, but are not limited to tartaric acid, lactic acid, succinic acid, as well as mandelic acid, atrolactic acid, methanesulfonic acid, ethanesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, carbonic acid, fumaric acid, gluconic acid, acetic acid, propionic acid, salicylic acid, hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, citric acid, or hydroxybutyric acid, camphorsulfonic acid, malic acid, phenylacetic acid, aspartic acid, glutamic acid, and the like.
  • the invention contemplates pharmaceutically active compounds either chemically synthesized or formed by in vivo biotransformation to compounds of formula (III).
  • DMSO dimethyl sulfoxide
  • HPLC high-pressure liquid chromatography
  • PA polymer supported
  • FBS fetal bovine serum
  • HBSS Hank's Balanced Salt Solution
  • HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
  • PBS phosphate buffered saline
  • SDS sodium dodecyl sulfate
  • Tween polyoxoethylenesorbitan monolaurate
  • HBC (2-hydroxypropyl)- ⁇ -cyclodextrin.
  • the resulting material was purified by either flash chromatography [Analogix prepacked silica gel cartridges, 5-50% gradient of ethyl acetate/hexanes], or by preparative HPLC [Waters Xterra® RP 18 30x100 mm column, flow rate 40 mL/min, 5-95% gradient of acetonitrile in buffer (0.1 M aqueous ammonium bicarbonate, adjusted to pH 10 with sodium hydroxide)] to afford the desired amide product as its free base.
  • the compound was purified on a Waters Symmetry® C 8 30x 100 mm column (flow rate 40 mL/min, 5-95% gradient of acetonitrile in 0.1 % aqueous trifluoroacetic acid) to afford the amide product after evaporation of solvent.
  • a sealed tube was charged with sodium 2-chloro-2,2-difluoroacetate (0.525 g, 3.44 mmol) and sodium bicarbonate (0.284 g, 3.38 mmol) in dimethylformamide (14 mL).
  • the vessel was purged with nitrogen, and 4-mercaptobenzoic acid (0.35 g, 2.270 mmol) was added.
  • the mixture was warmed to 80 °C for 3 hours.
  • the reaction mixture was then cooled and filtered to remove a precipitate.
  • the dimethylformamide was diluted with diethyl ether (200 mL) and dichloromethane (75 mL) and acidified to pH 4.8 with acetic acid (0.5 mL).
  • the resulting cloudy solution was extracted with a dilute solution of aqueous sodium chloride (3 x 150 mL) followed by brine (2 x 150 mL).
  • the titled compound was prepared from 4-(trifluoromethylthio)benzoic acid and N-(1-methyl-1H-indol-4-yl)methanamine as described for example 12.
  • the titled compound was prepared from 4-(trifluoromethylthio)benzoic acid and N-(2-methyl-1H-indol-5-yl)methanamine as described for example 12.
  • N-(1H-indol-5-ylmethyl)-4-[(trifluoromethyl)thio]benzamide (Example 7, 98.7 mg, 0.280 mmol) and formaldehyde (4.0 mL, 53.7 mmol) were dissolved in methanol (4.0 mL). The resulting solution was cooled to 0 °C in an ice bath. Sodium triacetoxyborohydride was added in portions to the chilled solution. After 30 minutes, the ice bath was removed and the reaction mixture was stirred at room temperature for 2 hours. The reaction mixture was made basic by addition of 4 N sodium hydroxide. Volatiles were removed under reduced pressure. The residue was partitioned between water and dichloromethane.
  • N-(1H-indol-5-ylmethyl)-4-[(trifluoromethyl)thio]benzamide (Example 7, 117 mg, 0.335 mmol) was dissolved in acetic acid (1 mL) and methanol (1 mL). Sodium cyanoborohydride (63 mg, 1.00 mmol) was added in one portion. The reaction mixture was stirred under dry nitrogen overnight. The reaction mixture was made basic by addition of 4 N sodium hydroxide. Volatiles were removed under reduced pressure. The residue was partitioned between water and dichloromethane. The organic layer was dried over magnesium sulfate, filtered and concentrated.
  • the titled compound is prepared according to method A from 4-(2,2,2-trifluoroethoxy)aniline (Maybridge) and 1 H -indol-5-ylacetic acid ( Naruto, S.; Yonemitsu, O. Chem. Pharm. Bull. (1972), 20 (10) p. 2163-2171 ).
  • the titled compound is prepared from 4-(difluoromethylthio)aniline and 1 H -indol-5-ylacetic acid according to method A.
  • the titled compound is prepared from Example 6 and acetyl chloride using the procedure described in IIIi, V. O. Synthesis 1979, 387-388 .
  • the titled compound is prepared from Example 19 and acetic anhydride using the procedure described in Bolton, R. E.; et al., J. Chem. Soc., Chem. Commun. 1985, 1775-1776 .
  • Examples 26-190 listed below are prepared as described in the Examples, Schemes, or by procedures well known to one skilled in the art.
  • the starting materials are either available from commercial sources or are prepared as described in the Examples, Schemes, or by procedures well known to one skilled in the art:
  • the compounds of the invention were evaluated according to the following assays. These include (i) Xenopus oocytes injected with ⁇ 7 NNR RNA or DNA and evaluation of compound effects on current responses evoked by acetylcholine or another agonist (ii) IMR-32 cells endogenously expressing ⁇ 7 NNRs and measuring Ca 2+ flux or membrane potential changes utilizing the fluorescence-imaging plate reader (FLIPR)-based assays and (iii) measurement of phospho-ERK activity using western blot assays. These assays allow for evaluation of allosteric modulators using Xenopus oocytes, cells or cell lines expressing endogenous or recombinant ⁇ 7 NNRs.
  • FLIPR fluorescence-imaging plate reader
  • X laevis oocytes were prepared for electrophysiological experiments as described in the literature (see for example, Briggs, C. A., et al., Neuropharmacology, 1995, 34: 583-590 ; Briggs, C. A., et al., Neuropharmacology, 1998, 37: 1095-1102 ). In brief, three to four lobes from ovaries of female adult X.
  • collagenase type 1A 2 mg/mL; Sigma
  • the oocytes were injected with about 4 ng to about 6 ng of human ⁇ 7 NNR cRNA, kept at about 18 °C in a humidified incubator in modified Barth's solution (90 mM NaCl, 1.0 mM KCI, 0.66 mM NaNO 3 , 2.4 mM NaHCO 3 , 10 mM HEPES, 2.5 mM sodium pyruvate, 0.74 mM CaCl 2 , 0.82 mM MgCl 2 , 0.5% (v/v) penicillin-streptomycin solution, pH 7.55) and used about 2 to 7 days after injection.
  • modified Barth's solution 90 mM NaCl, 1.0 mM KCI, 0.66 mM NaNO 3 , 2.4 mM NaHCO 3 , 10 mM HEPES, 2.5 mM sodium pyruvate, 0.74 mM CaCl 2 , 0.82 mM MgCl 2 , 0.5% (v/v)
  • the oocytes were bathed in Ba 2+ -OR2 solution (90 mM NaCl, 2.5 mM KCI, 2.5 mM BaCl 2 , 1.0 mM MgCl 2 , 5.0 mM HEPES, and 0.0005 mM atropine, pH 7.4) to prevent activation of Ca 2+ -dependent currents and held at -60 mV at room temperature (about 20 °C).
  • Test compounds were given for ⁇ 60 seconds before agonist application and subsequently in the presence of 0.1 mM ACh.
  • Figure 2 shows the concentration-response relationship for Example 10 in potentiating 0.1 mM ACh-evoked ⁇ 7 currents in oocytes.
  • the EC 50 value is 0.21 ⁇ M and the degree of potentiation is 91%.
  • allosteric modulator compounds described herein can be identified using a fluorescence-based throughput functional assay using cell lines such as IMR-32 neuroblastoma or primary dissociated neurons.
  • cell lines such as IMR-32 neuroblastoma or primary dissociated neurons.
  • ⁇ 7 selective agonists in the present assay selectively stimulate calcium responses only in the presence of PAMs. Any suitable selective ⁇ 7 agonist can be used.
  • Selective ⁇ 7 agonists from a range of structural types may be used such as those described in the literature including PNU-282987, SSR180711A and AR-R17779, and others described in earlier patent applications, such as 2-methyl-5-(6-phenyl-pyridazin-3-yl)-octahydro-pyrrolo[3,4-c]pyrrole (see for example, US 20050065178 ), 5-[6-(5-methyl-hexahydro-pyrrolo[3,4-c]pyrrol-2yl)-pyridazin-3-yl]-1 H -indole (see for example, US 20050065178 ), 3-[6-(1 H -indol-5-yl)-pyradazin-3-yloxy]-1-aza-bicyclo[2.2.2]octane (see for example, US 2005/0137204 and US 2005/0245531 ), and 4-(5-phenyl-[1,3,4]oxadiazol
  • IMR-32 neuroblastoma cells were grown to confluency in 162 cm 2 tissue culture flasks in minimum essential media supplemented with 10% FBS and I mM sodium pyruvate, 0.1 mM non-essential amino acids and 1% antibiotic-antimycotic. The cells were then dissociated using cell dissociation buffer and 100 ⁇ L of 3.5 x 10 5 cells/mL cell suspension was plated (about 75,000 - 100,000 cells/well) into black 96 well plates precoated with poly-D-lysine with a clear bottom and maintained for 24-48 hours in a tissue culture incubator at 37 °C under an atmosphere of 5% CO 2 : 95% air.
  • clonal cell lines or dissociated primary cortical neurons that express endogenous ⁇ 7 nicotinic receptors may also be used in this assay.
  • Calcium flux was measured using a calcium-3 assay kit (Molecular Devices, Sunnyvale, CA) or fluo-4.
  • a stock solution of the dye was prepared by dissolving each vial supplied by the vendor in Hank's balanced salt solution buffer (HBSS) containing 10 or 20 mM HEPES. The stock solution was diluted 1 :20 using the same buffer before use. The growth media was removed from the cells and loaded with 100 ⁇ L of the dye and incubated at room temperature for one to three hours.
  • HBSS Hank's balanced salt solution buffer
  • Fluorescence measurements were read simultaneously from all the wells by a Fluorometric Imaging Plate Reader (FLIPR) at an excitation wavelength of 480 nm and an emission wavelength of 520 nm. Baseline fluorescence was measured for the first 10 seconds at which 50 ⁇ L of 3x concentrations of modulator/test compounds were added to the cell plate and incubated for three to five minutes. The fluorescence intensity was captured every second for the first 1 minute followed by every 2-5 seconds for an additional two to four minutes. This procedure was followed by 50 ⁇ L of 4x concentration of agonist and readings were taken for a period of three to five minutes as described above.
  • the assay can also be adapted to other formats such as 384- or 1536-well formats.
  • PAMs were identified by measuring fluorescence changes to intracellular calcium in a fluorometric plate reader in the presence of a selective ⁇ 7 NNR agonist using cells natively expressing ⁇ 7 NNRs.
  • Compounds with PAM activity evoked a calcium fluorescence response in the IMR-32 neuroblastoma cell line, a cell line that expresses endogenous ⁇ 7 NNRs when the assay is conducted in presence of an ⁇ 7 NNR agonist.
  • the agonist alone did not evoke a calcium response. However, when the agonist and the modulator were co-applied together, calcium responses were triggered.
  • ⁇ 7 agonists including 2-methyl-5-(6-phenyl-pyridazin-3-yl)-octahydro-pyrrolo[3,4-c]pyrrole (published in US 20050065178 ), 5-[6-(5-methylhexahydro-pyrrolo[3,4-c]pyrrol-2yl)-pyridazin-3-yl]-1 H -indole (published in US 20050065178 ), various quinuclidine derivatives (see for example, US 2005/0137204 and US 2005/0245531 ) and PNU-282987 (see for example, Hajós, M., et al., J Pharmacol.
  • PC-12 cells Rat pheochromocytoma (PC-12) cells (ATCC, Manassas, VA) were cultured and maintained in F-12K media supplemented with 15% horse serum, 2.5% fetal calf serum, and 2 mM L-glutamine in poly-D lysine coated dishes at 37 °C and 5% CO 2 .
  • Cells were plated in black-walled clear bottom 96-well BiocoatTM plates coated with poly-D-lysine (BD Biosciences, Bedford, MA) and grown for 2-3 days. Afterward, the culture media is replaced with serum-free media to starve cells overnight.
  • D-PBS Dulbecco's phosphate buffer saline
  • PC-12 cells are treated for 10 minutes at 37 °C with test PAM compounds and then challenged with a selective ⁇ 7 agonist for 5 minutes at 37 °C in a final volume of 100 ⁇ L/well, unless otherwise indicated. After treatment, media was discarded and adherent cells were immediately fixed in the presence of 150 ⁇ L/well of 3.7% formaldehyde/phosphate-buffered saline for 30-60 minutes at room temperature. Cells were then washed (4 times/5 minutes) and permeabilized with 200 ⁇ L/well of 0.1% Triton X-100/PBS. Permeabilized cells were blocked using the Odyssey® blocking buffer (100 ⁇ L/well) and plates were rocked overnight at 4 °C.
  • Both anti-total ERK (rabbit) and anti-phospho ERK (mouse) antibodies were diluted to 1/1000 and 1/500, respectively, in Odyssey® blocking buffer and added together at 50 ⁇ L/well for 2-3 hours at room temperature.
  • Polyclonal rabbit anti-ERK1/2 and monoclonal mouse anti-phospho-ERK 1/2 were purchased from Sigma-Aldrich (St. Louis, MO). The plates were washed 4 times with 0.1 % Tween 20/PBS (200 uL/well), and incubated with secondary antibodies (1/1000 dilution) in blocking buffer supplemented with 0.2% Tween for 1 hour.
  • Alexa Floor® 680-labeled goat anti-rabbit antibodies were added to recognize total ERK labeling (red color) and IRDyeTM800-labeled donkey anti-mouse antibodies were added to recognize phospho-ERK labeling (green color).
  • Alexa Fluor® 680-labeled goat-anti-rabbit antibodies were obtained from Molecular Probes (Eugene, OR).
  • IRDyeTM 800CW-labeled donkey-anti-mouse antibodies were purchased from Rockland (Gilbertsville, PA). The plates were washed 4 times with 0.2% Tween and 0.01% SDS/PBS and scanned using the Odyssey® infrared scanner. Well intensities were quantitated and phospho-ERIC signals were normalized to total ERK signals by the Odyssey® software. Data analysis was performed using GraphPad Prism (GraphPad Software, San Diego, CA).
  • PAMs can be identified by measuring changes in the phosphorylation of ERK (extracellular receptor kinase) by in-cell western analysis. Compounds with allosteric modulator activity evoke concentration-dependent increases in ERK phosphorylation.
  • an ⁇ 7 NNR agonist such as PNU-282987 (see for example, Hajos et al. J Pharmacol. Exp Ther. 2005; 312: 1213-22 ) was used as the ⁇ 7 selective agonist.
  • Typical EC 50 values in this assay range from about 10 nM to about 30 ⁇ M.
  • ⁇ 7 nicotinic receptor agonists including 2-methyl-5-(6-phenyl-pyridazin-3-yl)-octahydro-pyrrolo[3,4-c]pyrrole, 5-[6-(5-methyl-hexahydro-pyrrolo[3,4-c]pyrrol-2yl)-pyridazin-3-yl]-1 H -indole (published in US 20050065178 ), various quinuclidine derivatives (see for example, US 2005/0137204 and US 2005/0245531 ) and 4-(5-Phenyl-[1,3,4]oxadiazol-2-yl)-1,4-diazabicyclo[3.2.2]nonane and related analogs (see for example, WO 2004/029053 ) also are suitable for the assay.
  • PAMs of ⁇ 7 NNR that can enhance the effects of a naturally occurring neurotransmitter, acetylcholine, or exogenously administered agonist.
  • PAMs generally amplify agonist (acetylcholine) responses by (i) attenuating receptor desensitization so that the receptor remains open for longer duration and/or (ii) by directly amplifying the efficacy of ACh by enhancing maximal receptor activation.
  • such compounds typically boost endogenous transmission of acetylcholine, and can do so in a temporally and spatially restricted manner since these effects will be localized to regions where the ⁇ 7 receptors are expressed.
  • Allosteric modulator compounds can modulate the function of ⁇ 7 NNRs by enhancing ion channel function as measured by calcium responses described herein, or other approaches such as current or membrane potential studies. Preferred compounds are those that behave as PAMs in these assays between a concentration range of about 0.1 nM to about 10 ⁇ M. Allosteric modulation of the ⁇ 7 receptor can trigger key signaling processes that are important to effects on memory, cytoprotection, gene transcription and disease modification. Therefore, the administration of a therapeutically effective amount of a compound of formula (III) to a mammal provides a method of selectively modulating the effects of ⁇ 7 NNRs.
  • Nociception was assessed using the formalin test.
  • the mice were placed in open plexiglass observation chambers for 30 minutes to allow them to acclimate to their surroundings; then they were removed for formalin administration. Mice were gently restrained while the dorsum of the hind paw was injected with 20 ⁇ L of 2.5% formalin into the plantar surface of the right hind paw with a 30-gauge needle. The animals were returned to the chambers and nociceptive behavior was observed immediately after formalin injection. Mirrors were placed in each chamber to enable unhindered observation. Nociceptive behavior was quantified as the time licking the injected paw for continuous 5 minutes (phase I) and 20-45 minutes (phase II), following formalin injection.
  • phase II Formalin-induced flinching/licking behavior was biphasic.
  • the initial acute phase (phase I, 0-5 minutes) was followed by a relatively short quiescent period, which was then followed by a prolonged tonic response (phase II, 20-45 minutes).
  • a reference PAM compound (N-(1H-indol-5-ylmethyl)-6-(2,2,2-trifluoroethoxy) nicotinamide at 10 mg/kg in 10% DMSO/HBC) administered subcutaneously 10 minutes prior to formalin injection has no anti-nociceptive activity observed in phase I, but reduced nociceptive behaviors of injected paw flicking duration by 55%, indicative of pain relief in this time period as shown in Scheme 3 indicating that the PAM compound might have inhibitory effect on formalin-induced central neuronal sensitization.

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Biomedical Technology (AREA)
  • Psychiatry (AREA)
  • Pain & Pain Management (AREA)
  • Addiction (AREA)
  • Rheumatology (AREA)
  • Hospice & Palliative Care (AREA)
  • Psychology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Pyridine Compounds (AREA)
  • Indole Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Quinoline Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)

Claims (9)

  1. Composé de la formule (III) :
    Figure imgb0018
     où
    R1 est un groupe alkylcarbonyle, un atome d'hydrogène, ou un groupe méthyle ;
    R2 est un atome d'hydrogène ou un groupe méthyle ;
    α est une liaison simple ou double ;
    X1 est N ou CH ;
    X2 est CH lorsque α est une liaison double, et X2 est CH2 ou CH2CH2- lorsque α est une liaison simple ;
    X3 et X4 sont indépendamment N ou CH ;
    T1 et T2 sont indépendamment un atome d'hydrogène, un groupe alkyle, aryle, cycloalkyle, halo, haloalkyle, ou R3-L2-, où au moins un de T1 et T2 est différent de l'atome d'hydrogène ;
    L2 est O ou S ; et
    R5 est un groupe alkyle, aryle, cycloalkyle, ou haloalkyle ;
    ou sel pharmaceutiquement acceptable de celui-ci.
  2. Composé selon la revendication 1, choisi dans le groupe constitué par :
    le 2-(1H-indol-5-yl)-N[6-(2,2,2-trifluoroéthoxy)pyridin-3-yl]acétamide ;
    le N-{4-[(difluorométhyl)thio]phényl}-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(4-isopropoxyphényl)acétamide ;
    le N-(4-éthoxyphényl)-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(4-méthoxyphényl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(4-propoxyphényl)acétamide ;
    le 2-(1H-indol-5-yl)-N-[4-(trifluorométhoxy)phényl]acétamide ;
    le 2-(1H-indol-5-yl)-N-[4-(2,2,2-trifluoroéthoxy)phényl]acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-isopropoxypyridine-3-yl)acétamide ;
    le N-(6-éthoxypyridin-3-yl)-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-méthoxypyridin-3-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-propoxypyridin-3-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-[6-(trifluorométhoxy)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-[6-(2,2,2-trifluoroéthoxy)pyridin)-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-phénoxypyridin-3-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-isopropoxypyridazin-3-yl)acétamide ;
    le N-(6-éthoxypyridazin-3-yl)-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-méthoxypyridazin-3-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-propoxypyridazin-3-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-[6-(4-méthylphénoxy)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-[5-(4-méthylphénoxy)pyridin-2-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-(5-isopropoxypyridine-2-yl)acétamide ;
    le N-(5-éthoxypyridin-2-yl)-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(5-méthoxypyridin-2-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(5-propoxypyridin-2-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-[5-(trifluorométhoxy)pyridin-2-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-[5-(2,2,2-trifluoroéthoxy)pyridin-2-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-(5-phénoxypyridin-2-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(4-isopropoxyphényl)acétamide ;
    le N-(4-éthoxyphényl)-2-(1H-indol-6-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(4-méthoxyphényl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(4-propoxyphényl)acétamide ;
    le 2-(1H-indol-6-yl)-N-[4-(trifluorométhoxy)phényl]acétamide ;
    le 2-(1H-indol-6-yl)-N-[4-(2,2,2-trifluoroéthoxy)phényl]acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-isopropoxypyridine-3-yl)acétamide ;
    le N-(6-éthoxypyridin-3-yl)-2-(1H-indol-6-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-méthoxypyridin-3-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-propoxypyridin-3-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-[6-(trifluorométhoxy)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-6-yl)-N-[6-(2,2,2-trifluoroéthoxy)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-phénoxypyridin-3-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-isopropoxypyridazin-3-yl)acétamide ;
    le N-(6-éthoxypyridazin-3-yl)-2-(1H-indol-6-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-méthoxypyridazin-3-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-propoxypyridazin-3-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-[6-(4-méthylphénoxy)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-6-yl)-N-[5-(4-méthylphénoxy)pyridin-2-yl]acétamide ;
    le 2-(1H-indol-6-yl)-N-(5-isopropoxypyridine-2-yl)acétamide ;
    le N-(5-éthoxypyridin-2-yl)-2-(1H-indol-6-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(5-méthoxypyridin-2-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(5-propoxypyridin-2-yl)acétamide ;
    le 2-(1H-indol-6-yl)-1-[5-(trifluorométhoxy)pyridin-2-yl]acétamide ;
    le 2-(1H-indol-6-yl)-N-[5-(2,2,2-trifluoroéthoxy)pyridin-2-yl]acétamide ;
    le 2-(1H-indol-6-yl)-N-(5-phénoxypyridin-2-yl)acétamide ;
    le N-(1H-indol-5-ylméthyl)-5-[(trifluorométhyl)thio]pyridine-2-carboxamide ;
    le 2-(1H-indol-5-yl)-N-{6-[(trifluorométhyl)thio]pyridin-3-yl}acétamide ;
    le 2-(1H-indol-5-yl)-N-{5-[(trifluorométhyl)thio]pyridin-2-yl}acétamide ;
    le 2-(1H-indol-5-yl)-N-{6-[(trifluorométhyl)thio]pyridazin-3-yl}acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-phénylpyridin-3-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(5-phénylpyridin-2-yl)acétamide ;
    le N-1,1'-biphényl-4-yi-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-(6-phénylpyridazin-3-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-{6-[(trifluorométhyl)thio]pyridin-3-yl}acétamide ;
    le 2-(1H-indol-6-yl)-N-{5-[(trifluorométhyl)thio]pyridin-2-yl}acétamide ;
    le 2-(1H-indol-6-yl)-N-{6-[(trifluorométhyl)thio]pyridazin-3-yl}acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-phénylpyridin-3-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(5-phénylpyridin-2-yl)acétamide ;
    le 2-(1H-indol-6-yl)-N-(6-phénylpyridazin-3-yl)acétamide ;
    le N-1,1'-biphényl-4-yl-2-(1H-indol-6-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-[4-(trifluorométhyl)phényl]acétamide ;
    le 2-(1H-indol-5-yl)-N-[5-(trifluorométhyl)pyridin-2-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-[6-(trifluorométhyl)pyridazin-3-yl]acétamide ;
    le N-(4-cyclopropylphényl)-2-(1H-indol-5-yl)acétamide ;
    le N-(6-cyclopropylpyridin-3-yl)-2-(1H-indol-5-yl)acétamide ;
    le N-(5-cyclopropylpyridin-2-yl)-2-(1H-indol-5-yl)acétamide ;
    le N-(6-cyclopropylpyridazin-3-yl)-2-(1H-indol-5-yl)acétamide ;
    le N-[4-(3-chlorophénoxy)phényl]-2-(1H-indol-5-yl)acétamide ;
    le N-[4-(2,5-dichlorophénoxy)phényl]-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-{4-[4-(trifluorométhoxy)phénoxy]phényl}acétamide ;
    le N-[4-(4-chlorophénoxy)phényl]-2-(1H-indol-5-yl)acétamide ;
    le N-[6-(3-chlorophénoxy)pyridin-3-yl]-2-(1H-indol-5-yl)acétamide ;
    le N-[6-(2,5-dichlorophénoxy)pyridin-3-yl]-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-{6-[4-(trifluorométhoxy)phénoxy]pyridin-3-yl}acétamide ;
    le N-[6-(4-chlorophénoxy)pyridin-3-yl]-2-(1H-indol-5-yl)acétamide ;
    le N-[5-(3-chlorophénoxy)pyridin-2-yl]-2-(1H-indol-5-yl)acétamide ;
    le N-[5-(2,5-dichlorophénoxy)pyridin-2-yl]-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-{5-[4-(trifluorométhoxy)phénoxy]pyridin-2-yl}acétamide ;
    le N-[5-(4-chlorophénoxy)pyridin-2-yl]-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-[6-(phénylthio)pyridin-3-yl]acétamide ;
    le N-{6-[(2,5-dichlorophényl)thio]pyridin-3-yl}-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indot-5-yl)-N-(6-{[3-triftuorométhoxy)phényl]thio}pyridin-3-yl)acétamide ;
    le 2-(2-méthyl-1H-indol-5-yl)-N-[6-(2,2,2-trifluoroéthoxy)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-{4-[(trifluorométhyl)thio]phényl}acétamide ;
    le N-{4-[(difluorométhyl)thio]phényl}-2-(1H-indol-5-yl)acétamide ;
    le 2-(1H-indol-5-yl)-N-[6-(2,2,2-trifluoroéthoxy)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-[6-(trifluorométhyl)pyridin-3-yl]acétamide ;
    le 2-(1H-indol-5-yl)-N-(4-phénoxyphényl)acétamide ;
    le 2-(1H-indol-5-yl)-N-{3-[(trifluorométhyl)thio]phényl}acétamide ; et
    le N-(6-chloropyridin-3-yl)-2-(1H-indol-5-yl)acétamide ;
    ou sel pharmaceutiquement acceptable de celui-ci.
  3. Composition pharmaceutique comprenant une quantité thérapeutiquement efficace du composé de la formule (III) selon la revendication 1 en combinaison avec un support pharmaceutiquement acceptable.
  4. Composé de la formule (III) selon la revendication 1 pour une utilisation dans le traitement d'un trouble ou d'un état choisi dans le groupe constitué par un trouble déficitaire de l'attention, un trouble d'hyperactivité déficitaire de l'attention, la maladie d'Alzheimer, une altération cognitive modérée, la démence sénile, la démence du SIDA, la maladie de Pick, la démence associée aux corps de Lewy, la démence associée au syndrome de Down, la sclérose latérale amyotrophique, la maladie de Huntington, une fonction réduite du SNC associée à une liaison traumatique du cerveau, la schizophrénie, une altération cognitive associée à la schizophrénie, la dépression, la douleur, la douleur aiguë, la douleur chronique, la douleur post-chirurgicale, l'inflammation, la douleur inflammatoire, la douleur neuropathique, la douleur ostéoarthritique, la douleur chronique du bas du dos, la migraine et l'arrêt du tabac, chez un patient en besoin d'un tel traitement, par administration d'une quantité thérapeutiquement efficace dudit composé de la formule (III) selon la revendication 1.
  5. Composé selon la revendication 4, dans lequel le trouble ou l'état est choisi dans le groupe constitué par la douleur, la douleur aiguë, la douleur chronique, la douleur post-chirurgicale, l'inflammation, la douleur inflammatoire, la douleur neuropathique, la douleur ostéoarthritique, la douleur chronique du bas du dos, et la migraine.
  6. Composé selon la revendication 4, comprenant de plus l'administration d'un composé de la formule (III) en combinaison avec un neuroleptique atypique, un agoniste nicotinique, des inhibiteurs de cholinestérase, des antagonistes d'histamine H3, des antagonistes de 5HT6, des agonistes de dopamine D3, des antagonistes de récepteur muscarinique ou des agents bloquants du canal du potassium, menant à une potentialisation des effets du sous-type de récepteur nicotonique α7.
  7. Composé de la formule (III) selon la revendication 1 pour une utilisation dans la détermination ou le diagnostic des états ou des troubles liés à l'activité de α7 NNR en laissant des formes isotopes-marquées de la formule (III) selon la revendication 1 interagir avec des cellules exprimant des α7 NNR endogènes ou des cellules exprimant des α7 NNR recombinants et en mesurant les effets de telles formes isotopes-marquées de composés sur lesdites cellules.
  8. Composé de la formule (III) selon la revendication 1 pour une utilisation dans l'identification d'un agoniste de α7 NNR en laissant le composé de la formule (III) selon la revendication 1 interagir avec des cellules ou des lignes cellulaires exprimant de manière endogène des α7 NNR ou des cellules exprimant des α7 NNR recombinants dans un milieu fluorescent et en mesurant les modifications d'une telle fluorescence.
  9. Composé selon la revendication 1 choisi parmi le N-[(1-acétyl-1H-indol-5-yl)méthyl]-6-(2,2,2-trifluoroéthoxy)nicotinamide ou le N[(1-acétyl-2,3-dihydro-1H-indol-5-yl)méthyl]-4-[(trifluorométhyl)thio]benzamide.
EP09707625.1A 2008-02-07 2009-02-06 Dérivés d'amide en tant que modulateurs allostériques positifs et procédés d'utilisation associés Active EP2250162B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2688608P 2008-02-07 2008-02-07
US14362809P 2009-01-09 2009-01-09
PCT/US2009/033329 WO2009100294A2 (fr) 2008-02-07 2009-02-06 Dérivés d'amide en tant que modulateurs allostériques positifs et procédés d'utilisation associés

Publications (3)

Publication Number Publication Date
EP2250162A2 EP2250162A2 (fr) 2010-11-17
EP2250162A4 EP2250162A4 (fr) 2012-10-31
EP2250162B1 true EP2250162B1 (fr) 2014-03-19

Family

ID=40952708

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09707625.1A Active EP2250162B1 (fr) 2008-02-07 2009-02-06 Dérivés d'amide en tant que modulateurs allostériques positifs et procédés d'utilisation associés

Country Status (17)

Country Link
US (1) US8536221B2 (fr)
EP (1) EP2250162B1 (fr)
JP (1) JP2011511800A (fr)
KR (1) KR20100124272A (fr)
CN (1) CN101990537A (fr)
AU (1) AU2009212259A1 (fr)
BR (1) BRPI0907492A2 (fr)
CA (1) CA2713519A1 (fr)
CO (1) CO6290763A2 (fr)
CR (1) CR11664A (fr)
DO (1) DOP2010000241A (fr)
EC (1) ECSP10010465A (fr)
IL (1) IL207332A0 (fr)
MX (1) MX2010008714A (fr)
RU (1) RU2010137114A (fr)
WO (1) WO2009100294A2 (fr)
ZA (1) ZA201005514B (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9108948B2 (en) 2006-06-23 2015-08-18 Abbvie Inc. Cyclopropyl amine derivatives
CN103382174A (zh) 2006-06-23 2013-11-06 雅培制药有限公司 作为组胺h3受体调节物的环丙胺衍生物
EA018113B1 (ru) * 2008-04-17 2013-05-30 Глэксо Груп Лимитед Индолы в качестве модуляторов никотинового рецептора ацетилхолина подтипа альфа-71
SG175084A1 (en) * 2009-04-16 2011-11-28 Proximagen Ltd Indole derivative modulators of the alpha 7 nachr
US9186353B2 (en) 2009-04-27 2015-11-17 Abbvie Inc. Treatment of osteoarthritis pain
WO2012037258A1 (fr) 2010-09-16 2012-03-22 Abbott Laboratories Procédés de préparation de dérivés cyclopropyliques substitués en 1,2
TW201245185A (en) 2011-04-01 2012-11-16 Lundbeck & Co As H New positive allosteric modulators of nicotinic acetylcholine receptor
AR086791A1 (es) 2011-07-01 2014-01-22 Lundbeck & Co As H Moduladores alostericos positivos del receptor de acetilcolina nicotinico
WO2014049133A1 (fr) * 2012-09-28 2014-04-03 H. Lundbeck A/S Nouveaux effecteurs allostériques positifs du récepteur nicotinique de l'acétylcholine
LT3071203T (lt) 2013-11-18 2021-05-25 Forma Therapeutics, Inc. Tetrahidrochinolino kompozicijos kaip bet bromodomeno inhibitoriai
EP3071205B1 (fr) 2013-11-18 2020-02-05 Forma Therapeutics, Inc. Compositions de benzopipérazine en tant qu'inhibiteurs de bromodomaines bet
EP3233087B1 (fr) 2014-12-16 2019-10-02 Axovant Sciences GmbH Composés amide de quinuclidine à substituants géminal, en tant qu'agonistes des récepteurs nicotiniques de l'acétylcholine 7
US10370370B2 (en) 2015-06-10 2019-08-06 Axovant Sciences Gmbh Aminobenzisoxazole compounds as agonists of α7-nicotinic acetylcholine receptors
US10428062B2 (en) 2015-08-12 2019-10-01 Axovant Sciences Gmbh Geminal substituted aminobenzisoxazole compounds as agonists of α7-nicotinic acetylcholine receptors
WO2017144183A1 (fr) * 2016-02-24 2017-08-31 Acesion Pharma Aps Nouveaux inhibiteurs des canaux potassiques
AR108586A1 (es) 2016-06-10 2018-09-05 Lilly Co Eli Compuestos de 2,3-dihidro-1h-indol
KR102348155B1 (ko) * 2016-07-18 2022-01-06 오에이티 아그리오 가부시키가이샤 신규한 벤질아미드 화합물, 이를 제조하는 방법, 및 살진드기제
WO2018051252A2 (fr) * 2016-09-15 2018-03-22 Insecticides (India) Limited Nouveau composé amide, son procédé de production et miticide
HU231478B1 (hu) 2018-07-13 2024-02-28 Richter Gedeon Nyrt. Szubsztituált (aza)indol származékok
HU231333B1 (hu) 2018-07-13 2023-01-28 Richter Gedeon Nyrt Spirokromán származékok
HU231414B1 (hu) 2018-07-13 2023-08-28 Richter Gedeon Nyrt. Tiadiazin származékok
WO2020096871A1 (fr) * 2018-11-06 2020-05-14 Merck Sharp & Dohme Corp. Nouveaux composés tricycliques substitués utilisés en tant qu'inhibiteurs de l'indoléamine 2,3-dioxygénase

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU707277B2 (en) 1995-12-14 1999-07-08 Merck & Co., Inc. Antagonists of gonadotropin releasing hormone
GB9919411D0 (en) 1999-08-18 1999-10-20 Zeneca Ltd Chemical compounds
US6306890B1 (en) 1999-08-30 2001-10-23 Vanderbilt University Esters derived from indolealkanols and novel amides derived from indolealkylamides that are selective COX-2 inhibitors
SE9903997D0 (sv) * 1999-11-03 1999-11-03 Astra Ab New compounds
DE10217006A1 (de) 2002-04-16 2003-11-06 Merck Patent Gmbh Substituierte Indole
DK1551835T3 (da) 2002-09-30 2007-06-04 Neurosearch As Hidtil ukendte 1,4-diazabicycloalkanderivater, deres fremstilling og anvendelse
CA2521907A1 (fr) * 2003-04-10 2004-10-21 Pfizer Inc. Composes bicycliques comme antagonistes du recepteur nr2b
US20050065178A1 (en) * 2003-09-19 2005-03-24 Anwer Basha Substituted diazabicycloakane derivatives
US20050137204A1 (en) * 2003-12-22 2005-06-23 Abbott Laboratories Fused bicycloheterocycle substituted quinuclidine derivatives
US20050245531A1 (en) * 2003-12-22 2005-11-03 Abbott Laboratories Fused bicycloheterocycle substituted quinuclidine derivatives
US20060106011A1 (en) * 2004-11-12 2006-05-18 Bock Mark G 2-(Bicyclo)alkylamino-derivatives as mediators of chronic pain and inflammation
US20100093767A1 (en) 2004-12-03 2010-04-15 Takeda San Diego, Inc. Mitotic Kinase Inhibitors
CA2660704A1 (fr) 2006-08-07 2008-02-14 Ironwood Pharmaceuticals, Inc. Composes d'indole

Also Published As

Publication number Publication date
DOP2010000241A (es) 2010-08-31
BRPI0907492A2 (pt) 2017-06-13
US20090270408A1 (en) 2009-10-29
KR20100124272A (ko) 2010-11-26
JP2011511800A (ja) 2011-04-14
MX2010008714A (es) 2010-09-24
US8536221B2 (en) 2013-09-17
EP2250162A4 (fr) 2012-10-31
CA2713519A1 (fr) 2009-08-13
CR11664A (es) 2011-01-06
IL207332A0 (en) 2010-12-30
WO2009100294A2 (fr) 2009-08-13
WO2009100294A3 (fr) 2009-11-12
EP2250162A2 (fr) 2010-11-17
ZA201005514B (en) 2012-01-25
RU2010137114A (ru) 2012-03-20
ECSP10010465A (es) 2010-10-30
CO6290763A2 (es) 2011-06-20
CN101990537A (zh) 2011-03-23
AU2009212259A1 (en) 2009-08-13

Similar Documents

Publication Publication Date Title
EP2250162B1 (fr) Dérivés d'amide en tant que modulateurs allostériques positifs et procédés d'utilisation associés
US7786171B2 (en) Amide derivatives as positive allosteric modulators and methods of use thereof
US8470813B2 (en) Thiazoline and oxazoline derivatives and their methods of use
US7741364B2 (en) Pyrrole derivatives and their methods of use
US8383657B2 (en) Thiazolylidine urea and amide derivatives and methods of use thereof
US20120252853A1 (en) Positive allosteric modulators of nicotinic acetylcholine receptor

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20100907

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA RS

RIN1 Information on inventor provided before grant (corrected)

Inventor name: MORTELL, KATHLEEN H.

Inventor name: NERSESIAN, DIANA L.

Inventor name: LEE, CHIH-HUNG

Inventor name: SCHRIMPF, MICHAEL R.

Inventor name: GOPALAKRISHNAN, MURALI

REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1150600

Country of ref document: HK

A4 Supplementary search report drawn up and despatched

Effective date: 20121002

RIC1 Information provided on ipc code assigned before grant

Ipc: C07D 403/12 20060101ALI20120926BHEP

Ipc: C07D 471/04 20060101ALI20120926BHEP

Ipc: C07D 401/12 20060101AFI20120926BHEP

Ipc: C07D 209/08 20060101ALI20120926BHEP

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ABBVIE INC.

RIC1 Information provided on ipc code assigned before grant

Ipc: C07D 401/12 20060101AFI20130604BHEP

Ipc: C07D 209/08 20060101ALI20130604BHEP

Ipc: C07D 403/12 20060101ALI20130604BHEP

Ipc: C07D 471/04 20060101ALI20130604BHEP

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20130814

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA RS

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 657601

Country of ref document: AT

Kind code of ref document: T

Effective date: 20140415

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602009022585

Country of ref document: DE

Effective date: 20140430

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140619

REG Reference to a national code

Ref country code: NL

Ref legal event code: VDEP

Effective date: 20140319

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 657601

Country of ref document: AT

Kind code of ref document: T

Effective date: 20140319

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: BE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140619

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140719

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602009022585

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140721

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

26N No opposition filed

Effective date: 20141222

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602009022585

Country of ref document: DE

Effective date: 20141222

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 602009022585

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150206

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20150206

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150228

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150228

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

Effective date: 20151030

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150206

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150206

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150901

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150302

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140620

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20090206

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319

REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1150600

Country of ref document: HK

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20140319