Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/02—Burettes; Pipettes
  • B01L3/0275—Interchangeable or disposable dispensing tips
  • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/02—Burettes; Pipettes
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/06—Fluid handling related problems
  • B01L2200/0689—Sealing

Definitions

• the invention relates to the field of laboratory pipettes, intended for the calibrated sampling and introduction of liquids into containers.
• Laboratory pipettes have one or more tips, generally removable, of substantially frustoconical exterior shape. Each of these tips is equipped with a removable and disposable plastic sampling cone. Conventionally, each of these cones is fitted onto the corresponding end piece and is held on the end piece by friction.
• the inner surface of the sampling cone has a substantially conical longitudinal section, the dimensions of which, in the zone where said friction with the tip must be achieved, must be such as to allow the cone to be fitted onto the tip and its fixing. with suitable holding force and tightness. Ejection of the cone is preferably carried out using a suitable device integrated into the pipette, such as, for example, that described in document FR-A-2 807 342.
• the force to be exerted for the ejection of the cone is random, and can be very high if the cone has been fitted onto the end piece with a significant force and depth of insertion.
• the aim of the invention is to provide a pipette tip that best solves the problems just mentioned.
• the invention relates to a sampling pipette tip, the substantially frustoconical or cylindrical end part of which is intended to receive by fitting a sampling cone, characterized in that said end part comprises at least two annular shoulders at the ends. levels at which the diameter of said end part decreases towards the lower end of the end piece, said shoulders providing at their levels a reduction in the diameter of said end part from 0.2 to 2mm, preferably 0.3 to 1.8mm .
• Said shoulders can be rounded.
• Said shoulders can then extend over a length of 0.2 to 5mm, preferably 0.5 to 3mm.
• the distance between the shoulders can be 2 to 8mm, preferably 3.5 to 6mm.
• Tip can be adapted for use on a multichannel pipette
• the subject of the invention is also a sampling pipette, characterized in that it comprises at least one tip of the above type.
• the invention can be a multichannel pipette.
• the invention is based on a new pipette tip configuration.
• the outer surface of its lower end portion comprises at least two annular stepped shoulders of dimensions chosen from a precise range, constituting preferred contact zones with the sampling cone.
• the lower shoulder seals the cone / tip contact.
• the upper shoulder essentially serves as an aid in centering the cone on the end piece and as a stop.
• FIG. 1 which shows, seen in longitudinal section, the end of an exemplary pipette tip according to the invention, equipped with its sampling cone;
• the tip example 1 according to the invention shown in Figure 1 has, as is known, in its lower end part 2, a generally frustoconical outer shape so as to allow the fitting on said end part 2 of a sampling cone 3, the interior space 4 of which is shaped with a corresponding frustoconical longitudinal section in the zone where contact with the tip 1 is to take place.
• This contact must have the best possible seal compatible with easy ejection of the cone 3 when the latter must be replaced.
• the cone 3 may have a completely usual configuration and dimensions, for example a strictly cylindrical or frustoconical inner surface, without hollow or landforms.
• constituent material which may be of any nature known to be suitable for the manufacture of pipette tips.
• the tip 1 according to the invention can be designed to equip a pre-existing pipette by simply replacing a removable tip of conventional configuration. It can also constitute a non-removable end of a pipette which, moreover, would be of the usual design.
• the end part 2 of the end piece 1 has two annular shoulders: an upper shoulder 5 and a lower shoulder 6. They each provide at their level a relatively sudden change in the outer diameter of the end piece 1, namely a reduction in this diameter in the direction of the lower end 8 of the nozzle 1. They successively come into contact with the inner wall 7 of the cone 3 when fitting the cone 3 on the nozzle 1, and allow ensure the function of maintaining the cone 3 on the end piece 1 with better efficiency than when, conventionally, the end part 2 of the end piece 1 has a diameter which constantly decreases progressively towards its lower end 8.
• shoulders 5, 6 each correspond to a reduction in the diameter of the end piece 1 which is from 0.2 to 2mm, preferably from 0.3 to 1.8mm.
• these shoulders 5, 6 do not have a sharp angle but are rounded to facilitate the fitting of the cone 3.
• the reduction in the diameter of the end piece 1 corresponding to a shoulder 5, 6 is carried out over a length from 0.2 to 5mm, preferably from 0.5 to 3mm.
• the main function of the upper shoulder 5 is to constitute both a centering member of the cone 3 during fitting and a progressive stop.
• the lower shoulder 6, for its part, essentially ensures the tightness of the cone 3 / end piece 1 connection, and it is at its level that the very localized deformation of the cone 3 caused by the fitting is strongest.
• the distance between the two shoulders 5, 6 is typically of the order of 2 to 8mm, preferably 3.5 to 6mm. The precise choices of these dimensions depend essentially on the characteristics of the cones which will be used. As shown, the cone 3 can, conventionally, contain a filter 9; but this characteristic, independent of the invention, is of course not at all compulsory.
• the invention makes it possible to make the geometry of the cone 3 / tip 1 contact more reliable, the quality of which thus becomes much less dependent than in the prior art on the force exerted by the user when fitting the cone 3. Due to the fact , in particular, the existence of the stop constituted by the upper shoulder 5, a high fitting force does not lead to a depression of the end part 2 of the end piece 1 in the cone 3 substantially greater than that provided by less fitting effort.
• this contact pressure is very variable from one tip to another and that the highest pressures are reached, as is generally observed, for the tips located in the lateral zones of the pipette.
• Curve 14 shows this same contact pressure, measured at the level of the sealing zones of each of the 12 tips of a multichannel pipette whose tips are shaped according to the invention and whose cones are identical to those of the previous conventional pipette. . It can be seen that: - this contact pressure is situated at values which are appreciably higher than for the conventional pipette; this is due, as has been said, to the smaller surface area of the sealing zone;
• the pipette tip according to the invention can be used for single-channel or multi-channel pipettes.
• the typical characteristics of the invention can be used indifferently in the two cases.
• the other characteristics of the tip are the usual characteristics making them suitable for use on either of these types of pipettes.

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• Health & Medical Sciences (AREA)
• Clinical Laboratory Science (AREA)
• Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Devices For Use In Laboratory Experiments (AREA)
• Sampling And Sample Adjustment (AREA)

Applications Claiming Priority (2)

Publications (1)

Family

ID=34954211

Family Applications (1)

Country Status (11)

Families Citing this family (8)

Family Cites Families (12)

• 2005
  • 2005-02-18 FR FR0501713A patent/FR2882273B1/fr active Active
• 2006
  • 2006-02-03 RU RU2007134513/04A patent/RU2388538C2/ru not_active IP Right Cessation
  • 2006-02-03 MX MX2007010056A patent/MX2007010056A/es not_active Application Discontinuation
  • 2006-02-03 EP EP06709248A patent/EP1855804A1/de not_active Ceased
  • 2006-02-03 AU AU2006215489A patent/AU2006215489A1/en not_active Abandoned
  • 2006-02-03 CN CN2006800052654A patent/CN101119803B/zh active Active
  • 2006-02-03 KR KR1020077020982A patent/KR101236589B1/ko active IP Right Grant
  • 2006-02-03 CA CA2598337A patent/CA2598337C/en active Active
  • 2006-02-03 WO PCT/FR2006/000259 patent/WO2006087444A1/fr active Application Filing
  • 2006-02-03 JP JP2007555658A patent/JP4977626B2/ja active Active
• 2007
  • 2007-08-14 US US11/838,384 patent/US8480972B2/en active Active

Non-Patent Citations (1)

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