EP1807694A1 - Stapelbares chromatographiemodul und einen stapel solcher module umfassende chromatographiesäule - Google Patents
Stapelbares chromatographiemodul und einen stapel solcher module umfassende chromatographiesäuleInfo
- Publication number
- EP1807694A1 EP1807694A1 EP04805387A EP04805387A EP1807694A1 EP 1807694 A1 EP1807694 A1 EP 1807694A1 EP 04805387 A EP04805387 A EP 04805387A EP 04805387 A EP04805387 A EP 04805387A EP 1807694 A1 EP1807694 A1 EP 1807694A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- module
- chromatography
- container
- modules
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6069—Construction of the column body with compartments or bed substructure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/291—Gel sorbents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6034—Construction of the column joining multiple columns
- G01N30/6039—Construction of the column joining multiple columns in series
Definitions
- Stackable chromatography module and chromatography column comprising a stack of such modules
- the invention relates to liquid chromatography.
- the liquid chromatography is conventionally carried out in a column containing a chromatographic mixture - comprising for example a gel consisting of microparticles in suspension in a buffer solution - contained in a cylindrical drum which is supplied with liquid to be chromatographed, this liquid being then recovered at the exit of the column.
- a chromatographic mixture - comprising for example a gel consisting of microparticles in suspension in a buffer solution - contained in a cylindrical drum which is supplied with liquid to be chromatographed, this liquid being then recovered at the exit of the column.
- the technical solution proposed by this document is not entirely satisfactory.
- the chromatography modules described are of relatively complex construction.
- the structure of the device poses problems of supply and distribution of the liquid to be chromatographed between the juxtaposed modules.
- the complex path followed by the liquid to be chromatographed limits the flow rate of the device.
- the invention aims in particular to overcome the aforementioned drawbacks, by providing a chromatography module to facilitate the assembly and disassembly of the chromatography column while ensuring good quality chromatography.
- the invention proposes a chromatography module comprising:
- a container having an upper end and a lower end; in the container, a chromatographic mixture;
- a filter membrane disposed at one end of the container; this module being characterized in that the ends of the container are open and of complementary shapes, and in that the filtering membrane is fixed on the lower end of the container.
- Such a module easy to handle, perme 't the direct storage of the chromatographic mixture. Its structure allows, by direct fitting on an identical module, to produce a chromatography column whose chromatographic mixture, monobloc, allows chromatography in a single step.
- the invention also relates to a chromatography column, comprising a stack of such modules, nested.
- FIG. 1 is a sectional elevational view showing a chromatography module according to the invention
- Figure 2 is a sectional elevational view of a container for a chromatography module as shown in Figure 1;
- FIG. 3 is a sectional elevational view of a chromatography column according to the invention formed by stacking chromatography modules such as that shown in Figure 1.
- FIG. 1 shows a chromatography module 1.
- This module 1 comprises a container 2, shown alone in FIG. 2, which comprises a cylindrical side wall 3 having a symmetry of revolution about a central axis A.
- This symmetry of revolution, under which the side wall 3 has a circular cross section, is not a necessity, any type of section (square, rectangular, oval, etc.) can be envisaged.
- the container 2 preferably made of a plastic material such as polypropylene, has an upper end 4 and a lower end 5 both open, each formed by a circular free edge, respectively upper ⁇ and lower 7.
- the container 2 delimits an enclosure 8, the volume of which is denoted by V1, in which a chromatographic mixture 9 - also called a stationary phase - comprising for example a chromatographic gel consisting of microparticles (such as microspheres) is received. ) in suspension in a buffer solution, for example in a ratio 1: 1 (that is to say one kilogram of microparticles per 1 liter of buffer solution).
- a filter membrane 10 whose diameter corresponds to the internal diameter of the wall 3, is attached to the lower end 5 of the container 2.
- This membrane 10 made for example of polyethylene, has a porous structure and ensures the retention of the chromatographic mixture 9 in the container 2.
- the container 2 has, on the side of the upper end 4, a shoulder 11 disposed at a distance from the edge upper 4, so as to form a woolen 12 whose inner diameter corresponds to the outer diameter of the container 2 at its lower end 5.
- the counterbore 12 has, in the chamber 8, a predetermined secondary volume V2 as a function of the compressibility characteristics of the chromatographic mixture 9 and of the desired final pressure thereof, as will appear in the following.
- the ends 4, 5 of the container are thus of complementary shapes, so that it is possible to stack several modules 1 by fitting them into each other by insertion of the lower end 5 of a first module 1 into the upper end 4 of a second module 1 until the lower edge 7 of the first abuts against the shoulder 11 of the second.
- the container 2 has, on the side of its upper end 5, a flange 13 (or lugs) projecting radially, in which a plurality of axial openings 14 are provided allowing the introduction of tightening ties 15, as will be explained below.
- the module 1 has a thickness - that is to say the distance separating its edges 6, 7 - between 50 mm and 100 mm, whereas its diameter (that is to say the diameter outside of the wall) is between 100 mm and '2000 mm.
- the illustrated example shows a modulus 1 relatively "square", but it could be much flatter in the event that its diameter would be chosen much higher than its thickness.
- Module 1 is constituted as follows.
- a chromatography column 19 is shown in FIG. 3.
- This column 19 comprises a stack of chromatographic modules 1 such as that which has just been presented, nested in each other in the manner previously described.
- the column 19 comprises, on either side of the modules 1, two closure plates, namely:
- a top plate 20 mounted on the last module at the upper end of the stack, said plate 'being provided with an orifice 21 feeding column 19 in liquid chromatograph - also known as mobile phase - and a nozzle 22 connected to this orifice, for the distribution of the liquid to be chromatographed over the entire surface of the upper end 4 of the module 1, and
- a bottom plate 23 mounted on the last module at the lower end of the stack, this plate 23 is also provided with an orifice 24 for discharging the liquid chromatographed from the column 19, and a nozzle 25 connected to this orifice 24 to group the liquid thereto.
- the pressure of the chromatographic mixture 9 has increased in proportion to the decrease in the volume occupied by the mixture 9. More precisely, if P2 is recorded as the pressure of the chromatographic mixture 9 in a stacked module 1, and if we neglect the thickness of the membrane 10, the pressure P2 verifies the following relation:
- the liquid to be chromatographed introduced by the upper plate 20, passes successively by all modules 1 of the stack, before being discharged through the lower plate 23. Thanks to the opening of the modules 1 at their ends, the flow of the mobile phase is laminar within the stationary phase 9, the membranes 10, which hold the latter in place within each module 1 taken separately, not opposing the transit of the mobile phase. Thus, everything happens within the column as if the stationary phase 9 was monoblock.
- the chromatographic mixture 9 will be chosen according to the type of chromatography that it is desired to carry out (ion exchange chromatography, gel filtration chromatography, hydrophobic chromatography, affinity chromatography, diatomaceous earth chromatography, etc. ), for example for protein filtration, purification or preparation of reagents.
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/FR2004/002837 WO2006048514A1 (fr) | 2004-11-04 | 2004-11-04 | Module de chromatographie empilable et colonne de chromatographie comprenant une pile de tels modules |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1807694A1 true EP1807694A1 (de) | 2007-07-18 |
Family
ID=34959756
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP04805387A Withdrawn EP1807694A1 (de) | 2004-11-04 | 2004-11-04 | Stapelbares chromatographiemodul und einen stapel solcher module umfassende chromatographiesäule |
Country Status (5)
Country | Link |
---|---|
US (1) | US20060118471A1 (de) |
EP (1) | EP1807694A1 (de) |
JP (1) | JP2008519277A (de) |
CA (1) | CA2584372A1 (de) |
WO (1) | WO2006048514A1 (de) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4285387B2 (ja) * | 2004-03-31 | 2009-06-24 | 財団法人雑賀技術研究所 | 固相抽出カートリッジ |
DE102005024154B3 (de) * | 2005-05-23 | 2007-02-08 | Bischoff Analysentechnik und -geräte GmbH | Elemente zur Trennung von Substanzen durch Verteilung zwischen einer stationären und einer mobilen Phase und Verfahren zur Herstellung einer Trennvorrichtung |
JP3957078B1 (ja) * | 2005-05-24 | 2007-08-08 | ヘラマンタイトン株式会社 | カートリッジカラム |
FR2902799B1 (fr) | 2006-06-27 | 2012-10-26 | Millipore Corp | Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide |
EP1916522A1 (de) * | 2006-10-25 | 2008-04-30 | Agilent Technologies, Inc. | Säule mit getrennten Abschnitten stationärer Phase |
CA2721409A1 (en) * | 2008-04-25 | 2009-10-29 | Can Ozbal | Separation cartridges and methods for fabrication and use thereof |
US20090321338A1 (en) * | 2008-06-25 | 2009-12-31 | Millipore Corporation | Chromatography apparatus |
US20100190963A1 (en) * | 2008-12-16 | 2010-07-29 | Millipore Corporation | Stirred Tank Reactor And Method |
KR101477871B1 (ko) | 2009-07-30 | 2014-12-30 | 에프. 호프만-라 로슈 아게 | 이동식 크로마토그래피 컬럼 세퍼레이터 |
GB2480057A (en) * | 2010-05-04 | 2011-11-09 | Agilent Technologies Inc | Separation device having coupled separation device elements |
ES2754210T3 (es) | 2010-05-17 | 2020-04-16 | Emd Millipore Corp | Polímeros sensibles a estímulos para la purificación de biomoléculas |
EP2577289B1 (de) | 2010-06-03 | 2021-03-17 | Cytiva BioProcess R&D AB | Parallele anordnung von chromatographiesäulenmodulen |
US9950277B2 (en) | 2010-06-03 | 2018-04-24 | Ge Healthcare Bioprocess R&D Ab | Parallel assembly of chromatography column modules |
US20130306563A1 (en) | 2011-02-02 | 2013-11-21 | Hoffman-La Roche Inc. | Chromatography column support |
CN102218225B (zh) * | 2011-03-30 | 2013-01-30 | 西北大学 | 柱层析分离装置 |
WO2016015952A1 (en) * | 2014-07-28 | 2016-02-04 | Ge Healthcare Bio-Sciences Ab | Stackable chromatography column modules and flow control blocks |
GB201522177D0 (en) * | 2015-12-16 | 2016-01-27 | Ge Healthcare Bio Sciences Ab | Stackable chromatography column modules |
JP6690957B2 (ja) * | 2016-02-10 | 2020-04-28 | 日本メジフィジックス株式会社 | 放射性標識化合物の製造装置及び拘束具 |
Family Cites Families (29)
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US565890A (en) * | 1896-08-18 | Filter | ||
US2708519A (en) * | 1953-01-30 | 1955-05-17 | Ohio Commw Eng Co | Chromatographic adsorption apparatus with eluting solvent flow control valve |
US2708521A (en) * | 1954-06-14 | 1955-05-17 | Aero Supply Mfg Company Inc | Stacked disc type fluid filter |
DE2008354A1 (de) * | 1970-02-23 | 1971-09-09 | Boehnnger Mannheim GmbH, 6800 Mann heim | Kombinationssaule |
US3720322A (en) * | 1970-12-31 | 1973-03-13 | J Harms | Upflow cartridge filter apparatus |
US4155846A (en) * | 1977-10-19 | 1979-05-22 | Bowdle Paul H | Multi-segmented adsorption ion exchange or gell filtration column apparatus and process |
US4361486A (en) * | 1981-04-28 | 1982-11-30 | Amf Incorporated | Filter media, method for oxidizing and removing soluble iron, method for removing and inactivating microorganisms, and particulate filter aid |
SE8205033L (sv) * | 1982-09-03 | 1984-03-04 | Nils M L Engstrom | Delbar kromatografipelare med detekterings- och signalanordning samt elueringsystem - i kombination eller fristaende |
JPS59199032A (ja) * | 1983-04-26 | 1984-11-12 | Asahi Chem Ind Co Ltd | 圧力吸収機構 |
US4604198A (en) * | 1984-05-18 | 1986-08-05 | Amf Inc. | Multicartridge chromatography cartridge housing |
US4719011A (en) * | 1985-03-22 | 1988-01-12 | H. T. Chemicals, Inc. | High pressure liquid chromatography columns |
DE3519725A1 (de) * | 1985-06-01 | 1986-12-04 | Merck Patent Gmbh | Chromatographiesaeule |
US4676898A (en) * | 1985-11-04 | 1987-06-30 | Sepragen Corporation | Chromatography column using horizontal flow |
JPS6332368A (ja) * | 1986-07-25 | 1988-02-12 | Tosoh Corp | 高密度充填型の濾過装置 |
US4855046A (en) * | 1987-10-22 | 1989-08-08 | Napco, Inc. | Multi-canister, externally-connected ion removal system |
CH674315A5 (de) * | 1987-11-23 | 1990-05-31 | Werner Hafner | |
JPH0299345U (de) * | 1989-01-26 | 1990-08-08 | ||
US5433847A (en) * | 1989-11-01 | 1995-07-18 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Radial flow chromatography |
US5296137A (en) * | 1992-03-12 | 1994-03-22 | Oil-Dri Corporation Of America | Filtration system with axial flow cartridge |
US5580523A (en) * | 1994-04-01 | 1996-12-03 | Bard; Allen J. | Integrated chemical synthesizers |
JPH0972890A (ja) * | 1995-09-04 | 1997-03-18 | Jeol Ltd | 液体クロマトグラフ用積層カラム |
JPH0989861A (ja) * | 1995-09-26 | 1997-04-04 | Suzuki Motor Corp | 液体クロマトグラフィ用カラム装置 |
US5667676A (en) * | 1996-05-01 | 1997-09-16 | Alaska; Andrew B. | Side-packed chromatographic column |
US5863428A (en) * | 1997-04-01 | 1999-01-26 | Phenomenex | Guard cartridge for chromatography |
JP3746605B2 (ja) * | 1997-12-26 | 2006-02-15 | 富士電機リテイルシステムズ株式会社 | 飲料水のディスペンサ |
US6248268B1 (en) * | 1998-11-16 | 2001-06-19 | Xc Corporation | Process of making microparticles of a thermally-gelled polysaccharide |
US6527951B1 (en) * | 2000-11-16 | 2003-03-04 | Waters Investments Limited | Chromatographic column |
US6500671B2 (en) * | 2001-02-05 | 2002-12-31 | The Board Of Regents Of The University Of Nebraska | Loading microcolums for the separation of analytes from a sample in the millisecond time scale |
JP2003337124A (ja) * | 2002-05-20 | 2003-11-28 | Sekisui Chem Co Ltd | 液体クロマトグラフ装置及び液体クロマトグラフ用カラム液体クロマト測定方法 |
-
2004
- 2004-11-04 CA CA002584372A patent/CA2584372A1/en not_active Abandoned
- 2004-11-04 EP EP04805387A patent/EP1807694A1/de not_active Withdrawn
- 2004-11-04 JP JP2007539601A patent/JP2008519277A/ja active Pending
- 2004-11-04 WO PCT/FR2004/002837 patent/WO2006048514A1/fr active Application Filing
-
2005
- 2005-11-04 US US11/266,493 patent/US20060118471A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
---|
See references of WO2006048514A1 * |
Also Published As
Publication number | Publication date |
---|---|
JP2008519277A (ja) | 2008-06-05 |
WO2006048514A1 (fr) | 2006-05-11 |
US20060118471A1 (en) | 2006-06-08 |
CA2584372A1 (en) | 2006-05-11 |
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Effective date: 20080729 |