EP1556044A2 - Compositions contenant des composes immunomodulateurs pour le traitement, la modification et le soulagement de la douleur et leurs methodes d'utilisation - Google Patents

Compositions contenant des composes immunomodulateurs pour le traitement, la modification et le soulagement de la douleur et leurs methodes d'utilisation

Info

Publication number
EP1556044A2
EP1556044A2 EP03777871A EP03777871A EP1556044A2 EP 1556044 A2 EP1556044 A2 EP 1556044A2 EP 03777871 A EP03777871 A EP 03777871A EP 03777871 A EP03777871 A EP 03777871A EP 1556044 A2 EP1556044 A2 EP 1556044A2
Authority
EP
European Patent Office
Prior art keywords
pain
alkyl
ofthe
immunomodulatory compound
immunomodulatory
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03777871A
Other languages
German (de)
English (en)
Inventor
Jerome B. Zeldis
Herbert Faleck
Donald C. Manning
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Celgene Corp
Original Assignee
Celgene Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celgene Corp filed Critical Celgene Corp
Publication of EP1556044A2 publication Critical patent/EP1556044A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
    • A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]

Definitions

  • This invention relates to methods of treating, preventing, modifying and managing pain, which comprise the administration of immunomodulatory compounds alone or in combination with known therapeutics.
  • the invention also relates to pharmaceutical compositions and dosing regimens.
  • the invention encompasses the use of immunomodulatory compounds in conjunction with neural blockade and/or other standard therapies for pain syndrome.
  • Pain is a leading symptom of many different disorders and is defined as an unpleasant sensory and emotional experience associated with actual or potential tissue damage or described in terms of such damage.
  • Pain leads to severe impairment of functional ability, which compromises the working, social, and family lives of sufferers. Around five percent ofthe adult population is estimated to suffer from pain sufficiently severe to cause significant disability.
  • Chojnowska E Stannard C. Epidemiology of Chronic Pain, Chapter 2, pp 15-26: T.S. Jensen, P.R. Wilson, A.S.C. Rice eds., Clinical Pain Management Chronic Pain, Arnold, London, 2003. In most pain conditions, there is an increased neural input from the periphery.
  • Sensory nerve impulses travel via the axons of primary afferent neurons to the dorsal horn ofthe spinal cord, where they propagate nerve impulses to dorsal horn neurons by releasing excitatory amino acids and neuropeptides at synapses.
  • Dorsal horn projection neurons process and transfer the information about a peripheral stimuli to the brain via ascending spinal pathways. Mannion, R.J. and Woolf, C.J., Clin. J. of Pain 7 ⁇ 5:S144-S156 (2000).
  • dorsal horn projection neurons The firing of dorsal horn projection neurons is determined not only by the excitatory input they receive, but also by inhibitory input from the spinal cord and higher nerve centers.
  • Several brain regions contribute to descending inhibitory pathways. Nerve fibers from these pathways release inhibitory substances such as endogenous opioids, ⁇ - aminobutyric acid ("GAB A"), and serotonin at synapses with other neurons in the dorsal horn, or primary afferent neurons and inhibit nociceptive transmission.
  • GABA A ⁇ - aminobutyric acid
  • Peripheral nerve injury can produce changes in dorsal horn excitability by down-regulating the amount of inhibitory control over dorsal horn neurons through various mechanisms.
  • Central sensitization may explain, in part, the continuing pain and hyperalgesia that occurs following an injury, and may serve an adaptive purpose by encouraging protection of the injury during the healing phase. Central sensitization, however, can persist long after the injury has healed thereby supporting chronic pain. Sensitization also plays a key role in chronic pain, helping to explain why it often exceeds the provoking stimulus, both spatially and temporally, and may help explain why established pain is more difficult to suppress than acute pain. Koltzenburg, M. Clin. J. of Pain 75.S131-S138 (2000). 2.1 TYPES OF PAIN
  • Nociceptive pain is elicited when noxious stimuli such as inflammatory chemical mediators are released following tissue injury, disease, or inflammation and are detected by normally functioning sensory receptors (nociceptors) at the site of injury. Koltzenburg, M. Clin. J. of Pain i(5:S131-S138 (2000). Clinical examples of nociceptive pain include but are not limited to pain associated with chemical or thermal burns, cuts and contusions ofthe skin, osteoarthritis, rheumatoid arthritis, tendonitis, and myofascial pain.
  • Nociceptors are distributed throughout the periphery of tissue. They are sensitive to noxious stimuli (e.g., thermal, mechanical, or chemical) which would damage tissue if prolonged. Activation of peripheral nociceptors by such stimuli excites discharges in two distinct types of primary afferent neurons: slowly conducting unmyelinated c-fibers and more rapidly conducting, thinly myelinated A ⁇ fibers. C-fibers are associated with burning pain and A ⁇ fibers with stabbing pain. Koltzenburg, M. Clin. J. of Pain 76:S131-S138 (2000); Besson, J.M. Lancet 555:1610-15 (1999); and Johnson, B.W.
  • noxious stimuli e.g., thermal, mechanical, or chemical
  • Pain Mechanisms Anatomy, Physiology and Neurochemistry, Chapter 11 in Practical Management of Pain ed. P. Prithvi Raj. (3 rd Ed., Mosby, Inc. St Louis, 2000). Most nociceptive pain involves signaling from both A ⁇ and c-types of primary afferent nerve fibers. Peripheral nociceptors are sensitized by inflammatory mediators such as prostaglandin, substance P, bradykinin, histamine, and serotonin, as well as by intense, repeated, or prolonged noxious stimulation. In addition, cytokines and growth factors (e.g., nerve growth factor) can influence neuronal phenotype and function. Besson, J.M. Lancet 555:1610-15 (1999).
  • nociceptors When sensitized, nociceptors exhibit a lower activation threshold and an increased rate of firing, which means that they generate nerve impulses more readily and more frequently. Peripheral sensitization of nociceptors plays an important role in spinal cord dorsal horn central sensitization and clinical pain states such as hyperalgesia and allodynia.
  • Inflammation also appears to have another important effect on peripheral nociceptors.
  • Some C-nociceptors do not normally respond to any level of mechanical or thermal stimuli, and are only activated in the presence of inflammation or in response to tissue injury. Such nociceptors are called “silent" nociceptors, and have been identified in visceral and cutaneous tissue. Besson, J.M. Lancet 555:1610-15 (1999); Koltzenburg, M. Clin. J. of Pain io ⁇ :S131-S138 (2000). Differences in how noxious stimuli are processed across different tissues contribute to the varying characteristics of nociceptive pain.
  • cutaneous pain is often described as a well-localized sharp, prickling, or burning sensation whereas deep somatic pain may be described as diffuse, dull, or an aching sensation.
  • pain perception and stimulus intensity there is a variable association between pain perception and stimulus intensity, as the central nervous system and general experience influence the perception of pain.
  • Neuropathic Pain reflects injury or impairment ofthe nervous system, and has been defined by the IASP as "pain initiated or caused by a primary lesion or dysfunction in the nervous system.”
  • IASP International Association for the Study of Pain (IASP) Task Force on Taxonomy, IASP Press: Seattle, 209-214, 1994.
  • Some neuropathic pain is caused by injury or dysfunction ofthe peripheral nervous system.
  • changes in the expression of key transducer molecules, transmitters, and ion channels occur, leading to altered excitability of peripheral neurons. Johnson, B.W.
  • neuropathic pain include but are not limited to pain associated with diabetic neuropathy, postherpetic neuralgia, trigeminal neuralgia, and post- stroke pain.
  • Neuropathic pain is commonly associated with several distinct characteristics, such as pain which may be continuous or episodic and is described in many ways, such as burning, tingling, prickling, shooting, electric-shock-like, jabbing, squeezing, deep aching, or spasmodic.
  • Paradoxically partial or complete sensory deficit is often present in patients with neuropathic pain who experience diminished perception of thermal and mechanical stimuli. Abnormal or unfamiliar unpleasant sensations (dysaesthesias) may also be present and contribute to patient suffering. Other features are the ability of otherwise non-noxious stimuli to produce pain (allodynia) or the disproportionate perception of pain in response to supra-threshold stimuli (hyperalgesia). Johnson, B.W. Pain Mechanisms: Anatomy, Physiology and Neurochemistry, Chapter 11 in Practical Management of Pain ed. P. Prithvi Raj. (3 rd Ed., Mosby, Inc. St Louis, 2000); and Attal, N. Clin. J. of Pain 16:S118-S130 (2000).
  • CRPS Complex regional pain syndrome
  • CRPS type I encompasses the condition known as reflex sympathetic dystrophy (RSD)
  • CRPS type II encompasses the condition known as causalgia and both types have subsets consistent with sympathetic maintained pain syndrome.
  • RSD reflex sympathetic dystrophy
  • CRPS type II encompasses the condition known as causalgia and both types have subsets consistent with sympathetic maintained pain syndrome.
  • a special consensus conference ofthe IASP addressed diagnosis and terminology ofthe disease, and endorsed the term CRPS with the two subtypes. Subsequent studies and conferences have refined the definitions such that the current guidelines give high sensitivity (0.70) with very high specificity (0.95).
  • CRPS is a multi-symptom and multi-system syndrome affecting multiple neural, bone and soft tissues, including one or more extremities, which is characterized by an intense pain.
  • CRPS remains poorly understood.
  • changes in peripheral and central somatosensory, autonomic, and motor processing, and a pathologic interaction of sympathetic and afferent systems have been proposed as underlying mechanisms.
  • Wasner et al. demonstrated a complete functional loss of cutaneous sympathetic vasoconstrictor activity in an early stage of CRPS with recovery.
  • CRPS Crohn's disease .
  • Various causes that have led to CRPS include but are not limited to head injury, stroke, polio, tumor, trauma, amylotrophic lateral sclerosis (ALS), myocardial infarction, polymyalgia rheumatica, operative procedure, brachial plexopathy, cast/splint immobilization, minor extremity injury and malignancy.
  • ALS amylotrophic lateral sclerosis
  • Symptoms of CRPS include but are not limited to pain, autonomic dysfunction, edema, movement disorder, dystrophy, and atrophy. Schwartzman R.J., NEngl JMed 343(9): 654-6 (2000). The pain is described as extremely severe and unrelenting, often with a burning character. Ninety percent of all CRPS patients complain of spontaneous burning pain and allodynia, which refers to pain with light touch. Much ofthe difficulty clinicians have with this syndrome is the fact that pain may be far worse than what would be expected based on physical findings. Id. Pain is also accompanied by swelling and joint tenderness, increased sweating, sensitivity to temperature and light touch, as well as color change to the skin. In fact, the diagnosis of CRPS cannot be made on reports of pain alone. Patients must have signs and symptoms of sensory abnormalities as well as vascular dysfunction accompanied by excessive sweating, edema or trophic changes to the skin.
  • CRPS type I also referred to as RSD
  • CRPS type II also referred to as causalgia
  • RSD CRPS type I
  • CRPS type II occurs after nerve injury.
  • CRPS is further divided into three distinct stages in its development and manifestation. However, the course ofthe disease seems to be so unpredictable between various patients that staging is not always clear or helpful in treatment. Schwartzman R.J., NEnglJMed 343(9): 654 (2000).
  • stage I Pain is more severe than would be expected from the injury, and it has a burning or aching quality. It may be increased by dependency ofthe limb, physical contact, or emotional upset.
  • the affected area typically becomes edematous, may be hyperthermic or hypothermic, and may show increased nail and hair growth.
  • Radiographs may show early bony changes. Id.
  • stage II or "established RSD”
  • edematous tissue becomes indurated. Skin typically becomes cool and hyperhidrotic with livedo reticularis or cyanosis. Hair may be lost, and nails become ridged, cracked, and brittle. Hand dryness becomes prominent, and atrophy of skin and subcutaneous tissues becomes noticeable. Pain remains the dominant feature. It is usually constant and is increased by any stimulus to the affected area. Stiffness develops at this stage. Radiographs may show diffuse osteoporosis. Id.
  • stage III Pain spreads proximally. Although it may diminish in intensity, pain remains a prominent feature. Flare-ups may occur spontaneously. Irreversible tissue damage occurs, and the skin is typically thin and shiny. Edema is absent, but contractures may occur. X-ray films typically indicate marked bone demineralization. Id. h all stages of CRPS, patients endure severe chronic pain and most patients are sleep deprived. CRPS has significant morbidity and thus raising awareness ofthe disease is important. Early and effective treatment may lessen the effect of CRPS in some individuals. William D. Dzwierzynski et al, Hand Clinics Nol 10 (1): 29-44 (1994). 2.1.3 Other Types of Pain
  • Visceral pain has been conventionally viewed as a variant of somatic pain, but may differ in neurological mechanisms. Visceral pain is also thought to involve silent nociceptors, visceral afferent fibers that only become activated in the presence of inflammation. Cervero, F. and Laird J.M.A., Lancet 555:2145-48 (1999).
  • Headaches can be classified as primary and secondary headache disorders.
  • the pathophysiology ofthe two most common primary disorders, i.e., migraine and tension-type headache, is complex and not fully understood.
  • nociceptive input to the CNS may be increased due to the activation and sensitization of peripheral nociceptors, and the barrage of nociceptive impulses results in the activation and sensitization of second- and third-order neurons in the CNS.
  • central sensitization plays a role in the initiation and maintenance of migraine and tension-type headache. Johnson, B.W.
  • Pain Mechanisms Anatomy, Physiology and Neurochemistry, Chapter 11 in Practical Management of Pain ed. P. Prithvi Raj. (3 rd Ed., Mosby, Inc. St Louis, 2000).
  • Post-operative pain such as that resulting from trauma to tissue caused during surgery, produces a barrage of nociceptive input.
  • cytokines, neuropeptides and other inflammatory mediators These chemicals are responsible for the sensitization and increased responsiveness to external stimuli, resulting in, for example, lowering ofthe threshold and an increased response to supra-threshold stimuli. Together, these processes result in peripheral and central sensitization.
  • Mixed pain is chronic pain that has nociceptive and neuropathic components. For example, a particular pain can be initiated through one pain pathway and sustained through a different pain pathway. Examples of mixed pain states include, but are not limited to, cancer pain and low back pain.
  • CRPS related pain includes pain management and extensive physical therapy, which can help to prevent edema and joint contractures and can also help to minimize pain.
  • medication and neural blockade are used to help with the severe pain.
  • Regional neural blockade is performed using Bier blocks with a variety of agents, including local anesthetics, bretylium, steroids, calcitonin, reserpine, and guanethidine.
  • Perez R.S. et al, JPain Symptom Manage 2001 Jun; 21(6): 511-26.
  • selective sympathetic ganglia neural blockade is performed for both diagnostic and therapeutic purposes. The rationale for selective neural blockade is to interrupt the sympathetic nervous system and reduce the activation ofthe sensory nerves.
  • CRPS sympathetic-independent neural blockade
  • pain is typically lifelong and may be severe enough to be debilitating.
  • Medications presently used during the treatment of chronic pain in general include non-narcotic analgesics, opioid analgesics, calcium channel blockers, muscle relaxants, and systemic corticosteroids.
  • the mechanisms of pain and autonomic dysfunction are poorly understood, the treatments are completely empirical. Between five and ten percent of patients with CRPS develop a chronic form of pain, often with severe disability and extensive use of pain medications. Therefore, there remains a need for safe and effective methods of treating and managing pain.
  • IMMUNOMODULATORY COMPOUNDS A group of compounds selected for their capacity to potently inhibit TNF-a production by LPS stimulated PBMC has been investigated. L.G. Corral, et al, Arm. Rheum. Dis. 58:(Suppl I) 1107-1113 (1999). These compounds, which are referred to as LMiDsTM (Celgene Corporation) or Immunomodulatory Drugs, show not only potent inhibition of TNF- ⁇ but also marked inhibition of LPS induced monocyte ILl ⁇ and IL12 production. LPS induced IL6 is also inhibited by immunomodulatory compounds, albeit partially. These compounds are potent stimulators of LPS induced IL10. Id.
  • This invention encompasses methods of treating, preventing, modifying or managing (e.g., lengthening the time of remission) pain, which comprise administering to a patient in need thereof a therapeutically or prophylactically effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof.
  • Another embodiment ofthe invention encompasses the use of one or more immunomodulatory compounds in combination with other therapeutics presently used to treat or prevent pain such as, but not limited to, antidepressants, antihypertensives, anxiolytics, calcium channel blockers, muscle relaxants, non-narcotic analgesics, opioid analgesics, alpha-adrenergic receptor agonists or antagonists, anti-inflammatory agents, cox-2 inhibitors, immunomodulatory agents, immunosuppressive agents, hyperbaric oxygen, JNK inhibitors and corticosteroids.
  • Yet another embodiment ofthe invention encompasses the use of one or more immunomodulatory compounds in combination with conventional therapies used to treat, prevent or manage pain including, but not limited to, surgery, interventional procedures (e.g., neural blockade), physical therapy, and psychological therapy.
  • the invention further encompasses pharmaceutical compositions, single unit dosage forms, and kits suitable for use in treating, preventing, modifying and/or managing pain, which comprise an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof.
  • neuromodulators by restoring the baseline or normal pain threshold ofthe injured animal of human to which they are administered.
  • compounds ofthe invention can act differently than analgesics, which typically diminish the response induced by stimulus, by instead altering the patient's ability to withstand that response either by suppressing the suffering associated with the pain or directly reducing the responsiveness ofthe nociceptors.
  • compounds disclosed herein can be used to treat, prevent, modify and manage not only norciceptive pain, but other types of pain (e.g., neuropathic pain) with substantially different etiologies.
  • a first embodiment ofthe invention encompasses methods of treating, preventing, modifying or managing pain, which comprise administering to a patient in need thereof a therapeutically or prophylactically effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof.
  • the invention further relates to the treatment, prevention, modification, or management of specific types of pain including, but not limited to, nociceptive pain, neuropathic pain, mixed pain of nociceptive and neuropathic pain, visceral pain, migraine, headache and post-operative pain.
  • nociceptive pain includes, but is not limited to, pain associated with chemical or thermal burns, cuts ofthe skin, contusions ofthe skin, osteoarthritis, rheumatoid arthritis, tendonitis, and myofascial pain.
  • neuroopathic pain includes, but is not limited to, CRPS type I, CRPS type II, reflex sympathetic dystrophy (RSD), reflex neurovascular dystrophy, reflex dystrophy, sympathetically maintained pain syndrome, causalgia, Sudeck atrophy of bone, algoneurodystrophy, shoulder hand syndrome, post-traumatic dystrophy, trigeminal neuralgia, post herpetic neuralgia, cancer related pain, phantom limb pain, fibromyalgia, chronic fatigue syndrome, spinal cord injury pain, central post-stroke pain, radiculopathy, diabetic neuropathy, post-stroke pain, luetic neuropathy, and other painful neuropathic conditions such as those induced by drugs such as vincristine, velcade and thalidomide.
  • RSD reflex sympathetic dystrophy
  • reflex neurovascular dystrophy reflex dystrophy
  • reflex dystrophy sympathetically maintained pain syndrome
  • causalgia Sudeck atrophy of bone
  • algoneurodystrophy shoulder hand syndrome
  • post-traumatic dystrophy trige
  • CRPS complex regional pain syndrome
  • CRPS and related syndromes mean a chronic pain disorder characterized by one or more ofthe following: pain, whether spontaneous or evoked, including allodynia (painful response to a stimulus that is not usually painful) and hyperalgesia (exaggerated response to a stimulus that is usually only mildly painful); pain that is disproportionate to the inciting event (e.g., years of severe pain after an ankle sprain); regional pain that is not limited to a single peripheral nerve distribution; and autonomic dysregulation (e.g., edema, alteration in blood flow and hyperhidrosis) associated with trophic skin changes (hair and nail growth abnormalities and cutaneous ulceration).
  • allodynia painful response to a stimulus that is not usually painful
  • hyperalgesia exaggerated response to a stimulus that is usually only mildly painful
  • pain that is disproportionate to the inciting event e.g., years of severe pain after an ankle sprain
  • regional pain that is not limited
  • Another embodiment ofthe invention encompasses methods of modifying or modulating the threshold, development and/or duration of pain which comprise administering to a patient in need of such modification or modulation a therapeutically or prophylactically effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof.
  • Another embodiment ofthe invention encompasses a pharmaceutical composition
  • a pharmaceutical composition comprising an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, and an optional carrier.
  • kits comprising single unit dosage forms comprising an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, and an optional carrier.
  • kits comprising a pharmaceutical composition comprising an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof.
  • the invention further encompasses kits comprising single unit dosage forms. Kits encompassed by this invention can further comprise additional active agents or combinations thereof.
  • one embodiment ofthe invention encompasses a method of treating, preventing, modifying and/or managing pain, which comprises administering to a patient in need thereof a therapeutically or prophylactically effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, and a therapeutically or prophylactically effective amount of a second active agent.
  • second active agents include, but are not limited to, conventional therapeutics used to treat or prevent pain such as antidepressants, anticonvulsants, antihypertensives, anxiolytics, calcium channel blockers, muscle relaxants, non-narcotic analgesics, opioid analgesics, anti-inflammatories, cox-2 inhibitors, immunomodulatory agents, alpha-adrenergic receptor agonists or antagonists, immunosuppressive agents, corticosteroids, hyperbaric oxygen, ketamine, other anesthetic agents, NMD A antagonists, and other therapeutics found, for example, in the Physician 's Desk Reference 2003.
  • conventional therapeutics used to treat or prevent pain such as antidepressants, anticonvulsants, antihypertensives, anxiolytics, calcium channel blockers, muscle relaxants, non-narcotic analgesics, opioid analgesics, anti-inflammatories, cox-2 inhibitors, immunomodulatory agents, alpha-adrenergic receptor
  • kits which comprise one or more immunomodulatory compounds, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, and a second active agent.
  • a kit may contain one or more compounds ofthe invention and an antidepressant, calcium channel blocker, non-narcotic analgesic, opioid analgesic, anti-inflammatory agent, cox-2 inhibitor, alpha-adrenergic receptor agonist or antagonist, immunomodulatory agent, immunosuppressive agent, anticonvulsant, or other drug capable of relieving or alleviating a symptom of pain.
  • another embodiment ofthe invention encompasses a method of reversing, reducing or avoiding an adverse effect associated with the administration of a second active agent in a patient suffering from pain, which comprises administering to a patient in need thereof a therapeutically or prophylactically effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof.
  • adverse effects include, but are not limited to, nausea, epigastric distress, vomiting, prolonged bleeding time, respiratory depression, metabolic acidosis, hyperthermia, uriticaria, bronchoconstriction, angioneurotic edema, and Reye's syndrome.
  • symptoms of pain may be treated with physical therapy, psychological therapy and certain types of surgery, such as, but not limited to, selective somatic or sympathetic ganglia neural blockade.
  • physical therapy such as, but not limited to, selective somatic or sympathetic ganglia neural blockade.
  • certain types of surgery such as, but not limited to, selective somatic or sympathetic ganglia neural blockade.
  • an immunomodulatory compound may provide a unique and unexpected synergy to reduce complications associated with conventional therapies.
  • this invention encompasses a method of treating, preventing, modifying and/or managing pain, which comprises administering to a patient (e.g., a human) an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, before, during, or after surgery (e.g., neural blockade), physical therapy, psychological therapy or other conventional, non-drug based therapies.
  • Compounds used in the invention include immunomodulatory compounds that are racemic, stereomerically enriched and stereomerically pure, and pharmaceutically acceptable salts, solvates, hydrates, stereoisomers, clathrates, and prodrugs thereof.
  • Preferred compounds used in the invention are small organic molecules having a molecular weight less than about 1000 g/mol, and are not proteins, peptides, oligonucleotides, oligosaccharides or other macromolecules.
  • stereomerically pure means a composition that comprises one stereoisomer of a compound and is substantially free of other stereoisomers of that compound.
  • a stereomerically pure composition of a compound having one chiral center will be substantially free ofthe opposite enantiomer ofthe compound.
  • a stereomerically pure composition of a compound having two chiral centers will be substantially free of other diastereomers ofthe compound.
  • a typical stereomerically pure compound comprises greater than about 80% by weight of one stereoisomer ofthe compound and less than about 20% by weight of other stereoisomers ofthe compound, more preferably greater than about 90% by weight of one stereoisomer ofthe compound and less than about 10% by weight ofthe other stereoisomers ofthe compound, even more preferably greater than about 95% by weight of one stereoisomer ofthe compound and less than about 5% by weight ofthe other stereoisomers ofthe compound, and most preferably greater than about 97% by weight of one stereoisomer ofthe compound and less than about 3% by weight ofthe other stereoisomers ofthe compound.
  • stereomerically enriched means a composition that comprises greater than about 60% by weight of one stereoisomer of a compound, preferably greater than about 70% by weight, more preferably greater than about 80% by weight of one stereoisomer of a compound.
  • enantiomerically pure means a stereomerically pure composition of a compound having one chiral center.
  • enantiomerically enriched means a stereomerically enriched composition of a compound having one chiral center.
  • immunomodulatory compounds or "JJVIiDsTM” (Celgene Corporation) used herein encompasses small organic molecules that markedly inhibit TNF-o LPS induced monocyte ILl ⁇ and IL12, and partially inhibit IL6 production.
  • one ofthe biological effects exerted by the immunomodulatory compounds is the reduction of synthesis of TNF-c .
  • Immunomodulatory compounds enhance the degradation of TNF- ⁇ mRNA.
  • immunomodulatory compounds may reduce allodynia and hyperalgesia in rats subjected to the chronic constriction injury model of neuropathic pain.
  • the compounds may also cause a long-term increase in spinal cord dorsal horn met-enkephalin, an important antinociceptive neurotransmitter.
  • immunomodulatory compounds used in the invention may also be potent co-stimulators of T cells and increase cell proliferation dramatically in a dose dependent manner.
  • Immunomodulatory compounds may also have a greater co-stimulatory effect on the CD8+ T cell subset than on the CD4+ T cell subset, hi addition, the compounds preferably have anti-inflammatory properties, and efficiently co-stimulate T cells.
  • immunomodulatory compounds include, but are not limited to, cyano and carboxy derivatives of substituted styrenes such as those disclosed in U.S. patent no. 5,929,117; l-oxo-2-(2,6-dioxo-3-fluoropiperidin-3yl) isoindolines and 1,3-dioxo- 2-(2,6-dioxo-3-fluoropiperidine-3-yl) isoindolines such as those described in U.S. patent nos. 5,874,448 and 5,955,476; the terra substituted 2-(2,6-dioxopiperdin-3-yl)-l- oxoisoindolines described in U.S. patent no.
  • analogs and derivatives of thahdomide including hydrolysis products, metabolites, derivatives and precursors of thahdomide, such as those described in U.S. patent nos. 5,593,990, 5,629,327, and 6,071,948 to D'Amato; aminothalidomide, as well as analogs, hydrolysis products, metabolites, derivatives and precursors of aminothalidomide, and substituted 2-(2,6- dioxopiperidin-3-yl) phthalimides and substituted 2-(2,6-dioxopiperidin-3-yl)-l- oxoisoindoles such as those described in U.S. patent nos.
  • immunomodulatory compounds include, but are not limited to, 1-oxo- and 1,3 dioxo-2-(2,6-dioxopiperidin-3-yl) isoindolines substituted with amino in the benzo ring as described in U.S. Patent no. 5,635,517 which is incorporated herein. These compounds have the structure I:
  • immunomodulatory compounds include, but are not limited to: 1 -oxo-2-(2,6-dioxopiperidin-3-yl)-4-aminoisoindoline; l-oxo-2-(2,6-dioxopiperidin-3-yl)-5-aminoisoindoline; l-oxo-2-(2,6-dioxopiperidin-3-yl)-6-aminoisoindoline; l-oxo-2-(2,6-dioxopiperidin-3-yl)-7-aminoisoindoline; 1 ,3-dioxo-2-(2,6-dioxopiperidin-3-yl)-4-aminoisoindoline; and l,3-dioxo-2-(2,6-dioxopiperidin-3-yl)-4-aminoisoindoline; and l,3-dioxo-2-(2,6-dioxopiperi
  • R 1 is hydrogen or methyl.
  • the invention encompasses the use of enantiomerically pure forms (e.g. optically pure (R) or (S) enantiomers) of these compounds.
  • R 2 is H, F, benzyl, (C ⁇ -C 8 )alkyl, (C 2 -C 8 )alkenyl, or (C 2 -C 8 )alkynyl; R 3 and R 3' are independently (C ⁇ -C 8 )alkyl, (C 3 -C 7 )cycloalkyl, (C 2 -C 8 )alkenyl, (C 2 -
  • C 8 alkynyl, benzyl, aryl, (Co-C 4 )alkyl-(C ⁇ -C 6 )heterocycloalkyl, (Co-C 4 )alkyl-(C 2 - C 5 )heteroaryl, (C 0 -C 8 )alkyl-N(R 6 ) 2 , (C ⁇ -C 8 )alkyl-OR 5 , (C ⁇ -C 8 )alkyl-C(O)OR 5 , (C ⁇ -C 8 )alkyl- O(CO)R 5 , or C(O)OR 5 ;
  • R 4 is (C ⁇ -C 8 )alkyl, (C 2 -C 8 )alkenyl, (C 2 -C 8 )alkynyl, (C ⁇ -C 4 )alkyl-OR 5 , benzyl, aryl, (Co-C 4 )alkyl-(C ⁇ -C 6 )heterocycloalkyl, or (C 0 -C 4 )alkyl-(C 2 -C5)heteroaryl;
  • R 5 is (Ci-C 8 )alkyl, (C 2 -C 8 )alkenyl, (C 2 -C 8 )alkynyl, benzyl, aryl, or (C 2 - C 5 )heteroaryl; each occurrence of R 6 is independently H, (C ⁇ -C 8 )alkyl, (C 2 -C 8 )alkenyl, (C 2 - C 8 )alkynyl, benzyl, aryl, (C 2 -C 5 )heteroaryl, or (Co-C 8 )alkyl-C(O)O-R 5 or the R 6 groups can join to form a heterocycloalkyl group; n is 0 or 1; and
  • R 1 is (C 3 -C 7 )cycloalkyl, (C 2 - C 8 )alkenyl, (C 2 -C 8 )alkynyl, benzyl, aryl, (C 0 -C 4 )alkyl-(C ⁇ -C 6 )heterocycloalkyl, (C 0 - C 4 )alkyl-(C 2 -C 5 )heteroaryl, C(O)R 3 , C(O)OR 4 , (C ⁇ -C 8 )alkyl-N(R 6 ) 2 , (C ⁇ -C 8 )alkyl-OR 5 , (Ci- C 8 )alkyl-C(O)OR 5 , C(S)NHR 3 , or (C ⁇ -C 8 )alkyl-O(CO)R 5 ;
  • R 2 is H or (C ⁇ -C 8 )alkyl
  • R 3 is (Ci-C 8 )alkyl, (C 3 -C 7 )cycloalkyl, (C 2 -C 8 )alkenyl, (C 2 -C 8 )alkynyl, benzyl, aryl, (C 0 -C 4 )alkyl-(Ci-C 6 )heterocycloalkyl, (Co-C 4 )alkyl-(C2-C 5 )heteroaryl, (C 5 -C 8 )alkyl-N(R 6 ) 2 ; (C 0 -C 8 )alkyl-NH-C(O)O-R 5 ; (C ⁇ -C 8 )alkyl-OR 5 , (C ⁇ -C 8 )alkyl-C(O)OR 5 , (C ⁇ -C 8 )alkyl- O(CO)R 5 , or C(O)OR 5 ; and the other variables have the same definitions.
  • R 2 is H or (C ⁇ -C )alkyl.
  • R 1 is (Ci-C 8 )alkyl or benzyl.
  • R 1 is H, (C ⁇ -C 8 )alkyl, benzyl, CH 2 OCH 3 CH 2 CH 2 OCH 3 , or
  • R 1 is
  • R 7 is independently H, (C ⁇ -C 8 )alkyl, benzyl, CH 2 OCH 3 , or CH 2 CH 2 OCH 3 .
  • R is C(O)R .
  • R 3 is (Co-C 4 )alkyl-(C2-Cs)heteroaryl, (G- Cs)alkyl, aryl, or (C 0 -C 4 )alkyl-OR 5 .
  • heteroaryl is pyridyl, furyl, or thienyl.
  • R 1 is C(O)OR 4 .
  • the H of C(O)NHC(O) can be replaced with (C ⁇ -C 4 )alkyl, aryl, or benzyl.
  • each of R 1 , R 2 , R 3 , or R 4 independently ofthe others, is halo, alkyl of 1 to 4 carbon atoms, or alkoxy of 1 to 4 carbon atoms or (ii) one of R 1 , R 2 , R 3 , or R 4 is nitro or - NHR 5 and the remaining of R 1 , R 2 , R 3 , or R 4 are hydrogen; R 5 is hydrogen or alkyl of 1 to 8 carbons
  • R 6 hydrogen, alkyl of 1 to 8 carbon atoms, benzo, chloro, or fluoro;
  • R' is R 7 -CHR 10 -N(R 8 R 9 );
  • R 7 is m-phenylene or p-phenylene or -(C n H 2n )- in which n has a value of 0 to 4; each of R 8 and R 9 taken independently ofthe other is hydrogen or alkyl of 1 to 8 carbon atoms, or R and R taken together are tetramethylene, pentamethylene, hexamethylene, or -CH 2 CH 2 [X]X ⁇ CH 2 CH 2 - in which [X]Xi is -O-, -S-, or -NH-; R 10 is hydrogen, alkyl of to 8 carbon atoms, or phenyl; and * represents a chiral-carbon center.
  • the most preferred immunomodulatory compounds are 4-(amino)-2-(2,6-dioxo(3- piperidyl))-isoindoline-l,3-dione and 3-(4-amino-l-oxo-l,3-dihydro-isoindol-2-yl)- piperidine-2,6-dione.
  • the compounds can be obtained via standard, synthetic methods (see e.g., United States Patent No. 5,635,517, incorporated herein by reference).
  • 4-(Amino)-2- (2,6-dioxo(3-piperidyl))-isoindoline-l,3-dione (ACTIMIDTM) has the following chemical structure:
  • Acceptable non-toxic acid addition salts include those derived from organic and inorganic acids or bases known in the art, which include, for example, hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, methanesulphonic acid, acetic acid, tartaric acid, lactic acid, succinic acid, citric acid, malic acid, maleic acid, sorbic acid, aconitic acid, salicylic acid, phthalic acid, embolic acid, enanthic acid, and the like.
  • organic and inorganic acids or bases known in the art, which include, for example, hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, methanesulphonic acid, acetic acid, tartaric acid, lactic acid, succinic acid, citric acid, malic acid, maleic acid, sorbic acid, aconitic acid, salicylic acid, phthalic acid, embolic acid, enanthic acid, and the like.
  • bases that can be used to prepare pharmaceutically acceptable base addition salts of such acidic compounds are those that form non-toxic base addition salts, i.e., salts containing pharmacologically acceptable cations such as, but not limited to, alkali metal or alkaline earth metal salts and the calcium, magnesium, sodium or potassium salts in particular.
  • Suitable organic bases include, but are not limited to, , N,N-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, ethylenediamine, meglumaine (N-methylglucamine), lysine, and procaine.
  • prodrug means a derivative of a compound that can hydrolyze, oxidize, or otherwise react under biological conditions (in vitro or in vivo) to provide the compound.
  • prodrugs include, but are not limited to, derivatives of immunomodulatory compounds that comprise biohydrolyzable moieties such as biohydrolyzable amides, biohydrolyzable esters, biohydrolyzable carbamates, biohydrolyzable carbonates, biohydrolyzable ureides, and biohydrolyzable phosphate analogues.
  • prodrugs include derivatives of immunomodulatory compounds that comprise -NO, -NO 2 , -ONO, or -ONO 2 moieties.
  • Prodrugs can typically be prepared using well-known methods, such as those described in 1 Burger's Medicinal Chemistry and Drug Discovery, 172-178, 949-982 (Manfred E. Wolff ed., 5th ed. 1995), and Design of Prodrugs (H. Bundgaard ed., Elselvier, New York 1985).
  • biohydrolyzable amide means an amide, ester, carbamate, carbonate, ureide, or phosphate, respectively, of a compound that either: 1) does not interfere with the biological activity ofthe compound but can confer upon that compound advantageous properties in vivo, such as uptake, duration of action, or onset of action; or 2) is biologically inactive but is converted in vivo to the biologically active compound.
  • biohydrolyzable esters include, but are not limited to, lower alkyl esters, lower acyloxyalkyl esters (such as acetoxylmethyl, acetoxyethyl, aminocarbonyloxymethyl, pivaloyloxymethyl, and pivaloyloxyethyl esters), lactonyl esters (such as phthalidyl and thiophthalidyl esters), lower alkoxyacyloxyalkyl esters (such as methoxycarbonyloxymethyl, ethoxycarbonyloxyethyl and isopropoxycarbonyloxyethyl esters), alkoxyalkyl esters, choline esters, and acylamino alkyl esters (such as acetamidomethyl esters).
  • lower alkyl esters such as acetoxylmethyl, acetoxyethyl, aminocarbonyloxymethyl, pivaloyloxymethyl, and pivaloyloxyethyl esters
  • biohydrolyzable amides include, but are not limited to, lower alkyl amides, ⁇ -amino acid amides, alkoxyacyl amides, and alkylaminoalkylcarbonyl amides.
  • biohydrolyzable carbamates include, but are not limited to, lower alkylamines, substituted ethylenediamines, aminoacids, hydroxyalkylamines, heterocyclic and heteroaromatic amines, and polyether amines. It should be noted that if there is a discrepancy between a depicted structure and a name given that structure, the depicted structure is to be accorded more weight.
  • a second active ingredient or agent can be used in the methods and compositions of the invention together with an immunomodulatory compound.
  • the second active agents are capable of relieving pain, inhibiting inflammatory reactions, providing a sedative effect or an antineuralgic effect, or ensuring patient comfort.
  • the second active agents include, but are not limited to, opioid analgesics, non-narcotic analgesics, anti-inflammatories, cox-2 inhibitors, alpha-adrenergic receptor agonists or antagonists, ketamine, anesthetic agents, NMDA antagonists, immunomodulatory agents, immunosuppressive agents, antidepressants, anticonvulsants, antihypertensives, anxiolytics, calcium channel blockers, muscle relaxants, corticosteroids, hyperbaric oxygen, JNK inhibitors, other therapeutics known to relieve pain, and pharmaceutically acceptable salts, solvates, hydrates, stereoisomers, clathrates, prodrugs and pharmacologically active metabolites thereof.
  • Opioids can be used to treat severe pain.
  • opioid analgesics include, but are not limited to, oxycodone (OxyContin ® ), morphine sulfate (MS Contin ® , Duramorph ® , Astramorph ® ), meperidine (Demerol ® ), and fentanyl transdermal patch (Duragesic ® ) and other known conventional medications; See, e.g., Physicians ' Desk Reference, 594-595, 2851 and 2991 (57 th ed., 2003).
  • Oxycodone (OxyContin ® ) is a long-acting form of an opioid and may be used usually in initial and later stages of CRPS.
  • Morphine sulfate may be used for analgesia due to reliable and predictable effects, safety profile, and ease of reversibility with naloxone. Morphine sulfate is sold in the United States under the trade name MS Contin ® , Duramorph ® , or Astramorph ® . See, e.g., Physicians' Desk Reference, 594-595 (57 th ed., 2003). Fentanyl transdermal patch (Duragesic ® ) is a potent narcotic analgesic with much shorter half-life than morphine sulfate. Meperidine (Demerol ® ) and hydromorphone (Dilaudid ® ) may also be used for pain management. See, e.g., Physicians ' Desk Reference, 2991 (57 th ed., 2003).
  • Non-narcotic analgesics and anti-inflammatories are preferably used for treatment of pain during pregnancy and breastfeeding.
  • Anti-inflammatories such as non-steroidal anti- inflammatory drugs (NSAIDs) and cox-2 inhibitors typically inhibit inflammatory reactions and pain by decreasing the activity of cyclo-oxygenase, which is responsible for prostaglandin synthesis. NSAIDs may provide pain relief in the early stage of pain syndrome.
  • NSAIDs non-steroidal anti- inflammatory drugs
  • cox-2 inhibitors typically inhibit inflammatory reactions and pain by decreasing the activity of cyclo-oxygenase, which is responsible for prostaglandin synthesis.
  • NSAIDs may provide pain relief in the early stage of pain syndrome.
  • anti-inflammatories examples include, but are not limited to, salicylic acid acetate (Aspirin ® ), ibuprofen (Motrin ® , Advil ® ), ketoprofen (Oruvail ® ), rofecoxib (Vioxx ® ), naproxen sodium (Anaprox ® , Naprelan ® , Naprosyn ® ), ketorolac (Acular ® ), and other known conventional medications.
  • a specific cox-2 inhibitor is celecoxib (Celebrex ® ).
  • Antidepressants increase the synaptic concentration of serotonin and/or norepinephrine in the CNS by inhibiting their reuptake by presynaptic neuronal membrane. Some antidepressants also have sodium channel blocking ability to reduce the firing rate of injured peripheral afferent fibers.
  • antidepressants include, but are not limited to, nortriptyline (Pamelor ® ), amitriptyline (Elavil ® ), imipramine (Tofranil ® ), doxepin (Sinequan ® ), clomipramine (Anafranil ® ), fluoxetine (Prozac ® ), sertraline (Zoloft ® ), nefazodone (Serzone ® ), venlafaxine (Effexor ® ), trazodone (Desyrel ® ), bupropion (Wellbutrin ® ) and other known conventional medications. See, e.g., Physicians ' Desk Reference, 329, 1417, 1831 and 3270 (57 th ed., 2003).
  • Anticonvulsant drugs may also be used in embodiments ofthe invention.
  • anticonvulsants include, but are not limited to, carbamazepine, oxcarbazepine, gabapentin (Neurontin ® ), phenytoin, sodium valproate, clonazepam, topiramate, lamotrigine, zonisamide, and tiagabine. See, e.g., Physicians ' Desk Reference, 2563 (57 th ed., 2003).
  • Corticosteroids e.g., prednisone, dexamethasone or hydrocortisone
  • orally active class lb anti-arrhythmic agents e.g., mexiletine
  • calcium channel blockers e.g., nifedipine
  • beta-blockers e.g., propranolol
  • alpha-blocker e.g., phenoxybenzamine
  • alpha2-adrenergic agonists e.g., clonidine
  • an immunomodulatory compound See, e.g., Physicians ' Desk Reference, 1979, 2006 and 2190 (57 th ed., 2003).
  • Specific second active agents used in the invention include, but are not limited to, salicylic acid acetate (Aspirin ® ), celecoxib (Celebrex ® ), Enbrel ® , ketamine, gabapentin (Neurontin ® ), phenytoin (Dilantin ® ), carbamazepine (Tegretol ® ), oxcarbazepine (Trileptal ® ), valproic acid (Depakene ® ), morphine sulfate, hydromorphone, prednisone, griseofulvin, penthonium, alendronate, dyphenhydramide, guanethidine, ketorolac
  • Methods of this invention encompass methods of preventing, treating, modifying and/or managing various types of pain.
  • preventing pain includes, but is not limited to, inhibiting or reducing the severity of one or more symptoms associated with pain.
  • Symptoms associated with pain include, but are not limited to, autonomic dysfunction, inability to initiate movement, weakness, tremor, muscle spasm, dytonia, dystrophy, atrophy, edema, stiffness, joint tenderness, increased sweating, sensitivity to temperature, light touch (allodynia), color change to the skin, hyperthermic or hypothermic, increased nail and hair growth, early bony changes, hyperhidrotic with livedo reticularis or cyanosis, lost hair, ridged, cracked or brittle nails, dry hand, diffuse osteoporosis, irreversible tissue damage, thin and shiny skin, joint contractures, and marked bone demineralization.
  • treating pain refers to the administration of a compound ofthe invention or other additional active agent after the onset of symptoms of pain
  • preventing refers to the administration prior to the onset of symptoms, particularly to patients at risk of pain.
  • patients at risk of pain include, but are not limited to, those who have incidents of trauma, neurologic disorder, myocardial infarction, musculoskeletal disorder and malignancy. Patients with familial history of pain syndromes are also preferred candidates for preventive regimens.
  • modifying pain encompasses modulating the threshold, development and duration of pain, or changing the way that a patient responds to pain. Without being limited by theory, it is believed that an immunomodulatory compound can act as an antihyperalgesic and/or neuromodulator.
  • "modifying pain” encompasses removing exaggerated pain response of a patient (i.e., a level at which a patient experiences greater than normal pain in response to a particular stimulus) and taking the system of a human or animal back towards a normal pain threshold.
  • "modifying pain” encompasses reducing a patient's pain response to a stimulus of a particular intensity.
  • modifying pain encompasses increasing a patient's pain threshold relative to the patient's pain threshold prior to the administration of an effective amount of an immunomodulatory compound.
  • managing pain encompasses preventing the recurrence of pain in a patient who had suffered from pain, and/or lengthening the time that a patient who had suffered from pain remains in remission.
  • the invention encompasses methods of treating, preventing, modifying and managing pain syndromes in patients with various stages and specific types ofthe disease, including, but not limited to, those referred to as nociceptive pain, neuropathic pain, mixed pain of nociceptive and neuropathic pain, visceral pain, migraine headache and postoperative pain.
  • Specific types of pain include, but are not limited to, pain associated with chemical or thermal burns, cuts ofthe skin, contusions ofthe skin, osteoarthritis, rheumatoid arthritis, or tendonitis, myofascial pain; CRPS type I, CRPS type II, reflex sympathetic dystrophy (RSD), reflex neurovascular dystrophy, reflex dystrophy, sympathetically maintained pain syndrome, causalgia, Sudeck atrophy of bone, algoneurodystrophy, shoulder hand syndrome, post-traumatic dystrophy, trigeminal neuralgia, post herpetic neuralgia, cancer related pain, phantom limb pain, fibromyalgia, chronic fatigue syndrome, spinal cord injury pain, central post-stroke pain, radiculopathy, diabetic neuropathy, post-stroke pain, luetic neuropathy, and other painful neuropathic conditions, e.g., painful neuropathic condition iatrogenically induced by drugs such as vincristine, velcade and thahdomide
  • the invention further encompasses methods of treating, modifying or managing pain in patients who have been previously treated for pain but were not sufficiently responsive or were non-responsive to standard therapy, as well as those who have not previously been treated for pain. Because patients with pain have heterogeneous clinical manifestations and varying clinical outcomes, the treatment, modification or management given to a patient may vary, depending on his/her prognosis. The skilled clinician will be able to readily determine without undue experimentation specific secondary agents, types of surgery, and types of physical therapy that can be effectively used to treat an individual patient.
  • Methods encompassed by this invention comprise administering one or more immunomodulatory compounds, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof to a patient (e.g., a human) suffering, or likely to suffer, from pain.
  • a patient e.g., a human
  • an immunomodulatory compound is administered orally and in single or divided daily doses in an amount of from about 0.10 to about 150 mg/day.
  • 4-(amino)-2-(2,6-dioxo(3-piperidyl))- isoindoline-l,3-dione is administered in an amount of from about 0.1 to 10 mg per day, or alternatively from about 0.1 to about 10 mg every other day or other syncopated regimen.
  • ActimidTM 4-(amino)-2-(2,6-dioxo(3-piperidyl))- isoindoline-l,3-dione
  • ActimidTM 4-(amino)-2-(2,6-dioxo(3-piperidyl))- isoindoline-l,3-dione
  • ActimidTM 4-(amino)-2-(2,6-dioxo(3-piperidyl))- isoindoline-l,3-dione
  • ActimidTM 4-(amino)-2-(2,6-dioxo(3-piperidyl))- iso
  • the invention relates to a method for treating, preventing, managing and/or modifying nociceptive pain, comprising administering an effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, to a patient in need thereof.
  • the nociceptive pain results from physical trauma (e.g., a cut or contusion of the skin; or a chemical or thermal burn), osteoarthritis, rheumatoid arthritis, or tendonitis.
  • the nociceptive pain is myofascial pain.
  • the invention in another embodiment, relates to a method for treating, preventing, managing and/or modifying neuropathic pain, comprising administering an effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, to a patient in need thereof.
  • the neuropathic pain is associated with stroke, diabetic neuropathy, luetic neuropathy, postherpetic neuralgia, trigeminal neuralgia, or painful neuropathy induced iatrogenically from drugs such as vincristine, velcade or thahdomide.
  • the invention relates to a method for treating, preventing, managing and/or modifying mixed pain (i.e., pain with both nociceptive and neuropathic components), comprising administering an effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, to a patient in need thereof.
  • mixed pain i.e., pain with both nociceptive and neuropathic components
  • Another embodiment ofthe invention comprises administering one or more immunomodulatory compounds, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, to a patient for treating, preventing, managing and/or modifying visceral pain, headache pain (e.g., migraine headache pain), CRPS type I, CRPS type II, RSD, reflex neurovascular dystrophy, reflex dystrophy, sympathetically maintained pain syndrome, causalgia, Sudeck atrophy of bone, algoneurodystrophy, shoulder hand syndrome, post-traumatic dystrophy, autonomic dysfunction, cancer-related pain, phantom limb pain, f ⁇ bromyalgia, chronic fatigue syndrome, post-operative pain, spinal cord injury pain, central post-stroke pain, or radiculopathy.
  • headache pain e.g., migraine headache pain
  • CRPS type I, CRPS type II, RSD reflex neurovascular dystrophy, reflex dystrophy, sympathetically maintained pain syndrome, causalgia, Sudeck atrophy
  • the invention relates to a method for treating, preventing, managing and/or modifying pain associated with a cytokine, comprising administering an effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, to a patient in need thereof.
  • an immunomodulatory compound or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof
  • inl ibiting cytokine activity or cytokine production results in the treatment, prevention, management and/or modification ofthe pain.
  • the cytokine is TNF- ⁇ .
  • the pain associated with a cytokine is nociceptive pain.
  • the pain associated with a cytokine is neuropathic pain.
  • the invention in another embodiment, relates to a method for treating, preventing, managing and/or modifying pain associated with inflammation, comprising administering an effective amount of an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, to a patient in need thereof.
  • the invention in another embodiment, relates to a method for treating, preventing, managing and/or modifying pain associated with a mitogen-activated protein kinase (MAPK), comprising administering an effective amount of an immunomodulatory compound to a patient in need thereof.
  • the MAPK is JNK (e.g. , JNKl , JNK2 or JNK3).
  • the MAPK is an extracellular signal-regulated kinase (ERK) (e.g., ERKl or ERK2).
  • the invention relates to a method of treating, preventing, managing and/or modifying pain associated with surgery, in one embodiment planned surgery (i. e. , planned trauma), comprising administering an effective amount of an immunomodulatory compound to a patient in need thereof.
  • the immunomodulatory compound can be administered before, during and/or after the planned surgery.
  • the patient is administered with about 5 to about 25 mg/day of an immunomodulatory compound from about 1-21 days prior to the planned surgery and/or about 5 to about 25 mg/day of an immunomodulatory compound from about 1-21 days after the planned surgery.
  • the patient is administered with about 10 mg/day of an immunomodulatory compound from about 1-21 days prior to the planned surgery and/or about 10 mg/day of an immunomodulatory compound from about 1-21 days after the planned surgery.
  • Specific methods ofthe invention comprise administering an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, in combination with a second active agent or active ingredient.
  • immunomodulatory compounds are disclosed herein (see, e.g., section 4.1); and examples of second active agents are also disclosed herein (see, e.g., section 4.2).
  • Administration ofthe immunomodulatory compounds and the second active agents to a patient can occur simultaneously or sequentially by the same or different routes of administration.
  • the suitability of a particular route of administration employed for a particular active agent will depend on the active agent itself (e.g. , whether it can be administered orally without decomposing prior to entering the blood stream) and the disease being treated.
  • a preferred route of administration for immunomodulatory compounds is oral.
  • Preferred routes of administration for the second active agents or ingredients ofthe invention are known to those of ordinary skill in the art. See, e.g., Physicians ' Desk Reference, 594-597 (57 th ed., 2003).
  • the second active agent is administered orally, intravenously, intramuscularly, subcutaneously, mucosally, or transdermally and once or twice daily in an amount of from about 1 to about 3,500 mg, from about 5 to about 2,500 mg, from about 10 to about 500 mg, or from about 25 to about 250 mg.
  • the specific amount ofthe second active agent will depend on the specific agent used, the type of pain being treated or managed, the severity and stage of pain, and the amount(s) of immunomodulatory compounds and any optional additional active agents concurrently administered to the patient.
  • the second active agent is salicylic acid acetate (Aspirin ® ), celecoxib (Celebrex ® ), Enbrel ® , Remicade ® , Humira ® , Kineret ® , ketamine, gabapentin (Neurontin ® ), phenytoin (Dilantin ® ), carbamazepine (Tegretol ® ), oxcarbazepine (Trileptal ® ), valproic acid (Depakene ® ), morphine sulfate, hydromorphone, prednisone, griseofulvin, penthonium, alendronate, dyphenhydramide, guanethidine, ketorolac (Acular ® ), thyrocalcitonin, dimethylsulfoxide (DMSO), clonidine (Catapress ® ), bretylium, ketanserin, reser
  • Hydromorphone (Dilaudid ® ) is preferably administered in an initial dose of about 2 mg orally, or about 1 mg intravenously to manage moderate to severe pain. See, e.g., Physicians ' Desk Reference, 2991 (57 th ed., 2003).
  • Morphine sulphate (Duramorph ® , Astramorph ® , MS Contin ® ) is preferably administered in an initial dose of about 2 mg IV/SC/LM, depending on whether a patient has already taken narcotic analgesics. See, e.g., Physicians ' Desk Reference, 594-595 (57 th ed., 2003).
  • Oxycodone (OxyContin ) is a long-acting form of an opioid and may be used in initial and later stages of pain syndrome.
  • Oxycodone (OxyContin ® ) is preferably administered in an amount of about 10-160 mg twice a day. See, e.g., Physicians ' Desk Reference, 2851 (57 th ed., 2003).
  • Meperidine (Demerol ® ) is preferably administered in an amount of about 50-150 mg PO/iN/IM/SC every 3-4 hours.
  • a typical pediatric dose of meperidine (Demerol ® ) is 1-1.8 mg/kg (0.5-0.8 mg/lb) PO/iN/LM/SC every 3-4 hours.
  • Fentanyl transdermal patch (Duragesic ® ) is available as a transdermal dosage form. Most patients are administered the drug in 72 hour dosing intervals; however, some patients may require dosing intervals of about 48 hours. A typical adult dose is about 25 mcg/h (10 cm ), 50 mcg/h (20 cm 2 ), 75 mcg/h (75 cm 2 ), or 100 mcg/h (100 cm 2 ). See, e.g., Physicians' Desk Reference, 1775 (57 th ed., 2003).
  • Non-narcotic analgesics and anti-inflammatories such as NSAIDs and cox-2 inhibitors may be used to treat patients suffering from mild to moderate pain.
  • Ibuprofen Motrin ® , Advil ®
  • Naproxen sodium (Anaprox ® , Naprelan ® , Naprosyn ® ) may also preferably be used for relief of mild to moderate pain in an amount of about 275 mg thrice a day or about 550 mg twice a day. See, e.g., Physicians' Desk Reference, 1417, 2193 and 2891 (57 th ed., 2003).
  • Antidepressants e.g., nortriptyline (Pamelor ® ), may also be used in embodiments of the invention to treat patients suffering from chronic and/or neuropathic pain.
  • the oral adult dose is typically in an amount of about 25-100 mg, and preferably does not exceed 200 mg/d.
  • a typical pediatric dose is about 0.1 mg/kg PO as initial dose, increasing, as tolerated, up to about 0.5-2 mg/d.
  • Amitriptyline (Etrafon ® ) is preferably used for neuropathic pain in an adult dose of about 25-100 mg PO. See, e.g., Physicians ' Desk
  • Anticonvulsants such as gabapentin (Neurontin ® ) may also be used to treat patients suffering from chronic and neuropathic pain.
  • gabapentin is orally administered in an amount of about 100- 1 ,200 mg three times a day.
  • Carbamazepine (Tegretol ® ) is used to treat pain associated with true trigeminal neuralgia.
  • the oral adult dose is typically in an amount of about 100 mg twice a day as initial dose, increasing, as tolerated, up to about 2,400 mg/d. See, e.g., Physicians ' Desk Reference, 2323-25 (57 th ed., 2003).
  • an immunomodulatory compound and a second active agent are administered to a patient, preferably a mammal, more preferably a human, in a sequence and within a time interval such that the immunomodulatory compound can act together with the other agent to provide an increased benefit than if they were administered otherwise.
  • the second active agent can be administered at the same time or sequentially in any order at different points in time; however, if not administered at the same time, they should be administered sufficiently close in time so as to provide the desired therapeutic or prophylactic effect.
  • the immunomodulatory compound and the second active agent exert their effect at times which overlap.
  • Each second active agent can be administered separately, in any appropriate form and by any suitable route.
  • the immunomodulatory compound is administered before, concurrently or after administration ofthe second active agent. Surgery can also be performed as a preventive measure or to relieve pain.
  • the immunomodulatory compound and the second active agent are administered less than about 1 hour apart, at about 1 hour apart, at about 1 hour to about 2 hours apart, at about 2 hours to about 3 hours apart, at about 3 hours to about 4 hours apart, at about 4 hours to about 5 hours apart, at about 5 hours to about 6 hours apart, at about 6 hours to about 7 hours apart, at about 7 hours to about 8 hours apart, at about 8 hours to about 9 hours apart, at about 9 hours to about 10 hours apart, at about 10 hours to about 11 hours apart, at about 11 hours to about 12 hours apart, no more than 24 hours apart or no more than 48 hours apart.
  • the immunomodulatory compound and the second active agent are administered concurrently.
  • the immunomodulatory compound and the second active agent are administered at about 2 to 4 days apart, at about 4 to 6 days apart, at about 1 week part, at about 1 to 2 weeks apart, or more than 2 weeks apart.
  • the immunomodulatory compound and optionally the second active agent are cyclically administered to a patient.
  • Cycling therapy involves the administration of a first agent for a period of time, followed by the administration of a second agent and/or third agent for a period of time and repeating this sequential administration. Cycling therapy can reduce the development of resistance to one or more of the therapies, avoid or reduce the side effects of one ofthe therapies, and/or improve the efficacy ofthe treatment.
  • the immunomodulatory compound and optionally the second active agent are administered in a cycle of less than about 3 weeks, about once every two weeks, about once every 10 days or about once every week.
  • One cycle can comprise the administration of an immunomodulatory compound and optionally the second active agent by infusion over about 90 minutes every cycle, about 1 hour every cycle, about 45 minutes every cycle.
  • Each cycle can comprise at least 1 week of rest, at least 2 weeks of rest, at least 3 weeks of rest.
  • the number of cycles administered is from about 1 to about 12 cycles, more typically from about 2 to about 10 cycles, and more typically from about 2 to about 8 cycles.
  • the immunomodulatory compound is administered in metronomic dosing regimens, either by continuous infusion or frequent administration without extended rest periods. Such metronomic administration can involve dosing at constant intervals without rest periods. Typically the immunomodulatory compounds, are used at lower doses. Such dosing regimens encompass the chronic daily administration of relatively low doses for extended periods of time. In preferred embodiments, the use of lower doses can minimize toxic side effects and eliminate rest periods, h certain embodiments, the immunomodulatory compound is delivered by chronic low-dose or continuous infusion ranging from about 24 hours to about 2 days, to about 1 week, to about 2 weeks, to about 3 weeks to about 1 month to about 2 months, to about 3 months, to about 4 months, to about 5 months, to about 6 months. The scheduling of such dose regimens can be optimized by the skilled artisan.
  • courses of treatment are administered concurrently to a patient, i.e., individual doses ofthe second active agent are administered separately yet within a time interval such that the immunomodulatory compound can work together with the second active agent.
  • one component can be administered once per week in combination with the other components that can be administered once every two weeks or once every three weeks.
  • the dosing regimens are carried out concurrently even if the therapeutics are not administered simultaneously or during the same day.
  • the second active agent can act additively or, more preferably, synergistically with the immunomodulatory compound.
  • an immunomodulatory compound is administered concurrently with one or more second active agents in the same pharmaceutical composition.
  • an immunomodulatory compound is administered concurrently with one or more second active agents in separate pharmaceutical compositions.
  • an immunomodulatory compound is administered prior to or subsequent to administration of a second active agent.
  • the invention contemplates administration of an immunomodulatory compound and a second active agent by the same or different routes of administration, e.g., oral and parenteral.
  • the second active agent can advantageously be administered at a dose that falls below the threshold that the adverse side effect is elicited.
  • this invention encompasses a method of treating, preventing, modifying and/or managing pain, which comprises administering an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, in conjunction with (e.g. before, during, or after) Pain Management interventional techniques.
  • Pain Management interventional techniques include, but are not limited to, the use of sympathetic blocks, intravenous regional blocks, placement of dorsal column stimulators or placement of intrathecal infusion devices for analgesic medication delivery.
  • Preferred Pain Management interventional techniques provides a selective neural blockade which interrupts the activity ofthe sympathetic nervous system in the region affected by pain.
  • the combined use ofthe immunomodulatory compounds and Pain Management interventional techniques may provide a unique treatment regimen that is unexpectedly effective in certain patients. Without being limited by theory, it is believed that immunomodulatory compounds may provide additive or synergistic effects when given concurrently with Pain Management interventional techniques.
  • Pain Management interventional techniques is intravenous regional block using BLER block with a variety of agents such as, but not limited to, local anesthetics such as , bupivacaine and lidocaine, guanethidine, ketamine, bretylium, steroids, ketorolac, and reserpine. Perez R.S., et al, JPain Symptom Manage 2001 Jun; 21(6): 511-26.
  • a stellate (cervicothoracic) ganglion block may be used.
  • the invention also encompasses the use of a somatic block, which involves continuous epidural infusion along with different variants of brachial plexus blocks.
  • An axillary, supraclavicular, or infraclavicular approach ofthe somatic block may also be useful.
  • this invention encompasses a method of treating, preventing, modifying and/or managing pain, which comprises administering an immunomodulatory compound, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, in conjunction with physical therapy or psychological therapy.
  • symptoms of pain include vasomotor dysfunction and movement disorders.
  • a steady progression of gentle weight bearing to progressive active weight bearing is very important in patients with pain syndromes. Gradual desensitization to increasing sensory stimuli may also be helpful. Gradual increase in normalized sensation tends to reset the altered processing in the CNS.
  • Physical therapy can thus play an important role in functional restoration. The goal of physical therapy is to gradually increase strength and flexibility.
  • immunomodulatory compounds may provide a unique treatment regimen that is unexpectedly effective in certain patients. Without being limited by theory, it is believed that immunomodulatory compounds may provide additive or synergistic effects when given concurrently with physical therapy.
  • immunomodulatory compounds may provide additive or synergistic effects when given concurrently with psychological therapy including, but not limited to, biofeedback, relaxation training, cognitive-behavioral therapy, and individual or family psychotherapy.
  • the immunomodulatory compound or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof is administered before, during, or after physical therapy or psychological treatment.
  • a second active agent is also administered to the patient.
  • compositions can be used in the preparation of individual, single unit dosage forms.
  • Pharmaceutical compositions and dosage forms ofthe invention comprise immunomodulatory compounds, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof.
  • Pharmaceutical compositions and dosage forms ofthe invention can further comprise one or more excipients.
  • compositions and dosage forms ofthe invention can also comprise one or more additional active ingredients. Consequently, pharmaceutical compositions and dosage forms ofthe invention comprise the active agents disclosed herein (e.g., immunomodulatory compounds, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, and a second active agent). Examples of optional additional active agents are disclosed herein (see, e.g., section 4.2).
  • Single unit dosage forms ofthe invention are suitable for oral, mucosal (e.g., nasal, sublingual, vaginal, buccal, or rectal), or parenteral (e.g., subcutaneous, intravenous, bolus injection, intramuscular, or intraarterial), transdermal or transcutaneous administration to a patient.
  • mucosal e.g., nasal, sublingual, vaginal, buccal, or rectal
  • parenteral e.g., subcutaneous, intravenous, bolus injection, intramuscular, or intraarterial
  • transdermal or transcutaneous administration to a patient.
  • dosage forms include, but are not limited to: tablets; caplets; capsules, such as soft elastic gelatin capsules; cachets; troches; lozenges; dispersions; suppositories; powders; aerosols (e.g., nasal sprays or inhalers); gels; liquid dosage forms suitable for oral or mucosal administration to a patient, including suspensions (e.g., aqueous or non-aqueous liquid suspensions, oil-in- water emulsions, or a water-in-oil liquid emulsions), solutions, and elixirs; liquid dosage forms suitable for parenteral administration to a patient; and sterile solids (e.g., crystalline or amorphous solids) that can be reconstituted to provide liquid dosage forms suitable for parenteral administration to a patient.
  • suspensions e.g., aqueous or non-aqueous liquid suspensions, oil-in- water emulsions, or a water-in-oil liquid e
  • composition, shape, and type of dosage forms ofthe invention will typically vary depending on their use.
  • a dosage form used in the acute treatment of a disease may contain larger amounts of one or more ofthe active agents it comprises than a dosage form used in the chronic treatment ofthe same disease.
  • a parenteral dosage form may contain smaller amounts of one or more ofthe active agents it comprises than an oral dosage form used to treat the same disease.
  • Typical pharmaceutical compositions and dosage forms comprise one or more excipients.
  • Suitable excipients are well known to those skilled in the art of pharmacy, and non-limiting examples of suitable excipients are provided herein. Whether a particular excipient is suitable for incorporation into a pharmaceutical composition or dosage form depends on a variety of factors well known in the art including, but not limited to, the way in which the dosage form will be administered to a patient.
  • oral dosage forms such as tablets may contain excipients not suited for use in parenteral dosage forms.
  • the suitability of a particular excipient may also depend on the specific active ingredients in the dosage form. For example, the decomposition of some active ingredients may be accelerated by some excipients such as lactose, or when exposed to water.
  • lactose-free compositions ofthe invention can comprise excipients that are well known in the art and are listed, for example, in the U.S. Pharmacopeia (USP) 25-NF20 (2002).
  • lactose-free compositions comprise active ingredients, a binder/filler, and a lubricant in pharmaceutically compatible and pharmaceutically acceptable amounts.
  • Preferred lactose-free dosage forms comprise active ingredients, microcrystalline cellulose, pre-gelatinized starch, and magnesium stearate.
  • This invention further encompasses anhydrous pharmaceutical compositions and dosage forms comprising active ingredients, since water can facilitate the degradation of some compounds.
  • water e.g., 5%
  • water is widely accepted in the pharmaceutical arts as a means of simulating long-term storage in order to determine characteristics such as shelf-life or the stability of formulations over time. See, e.g., Jens T. Carstensen, Drug Stability: Principles & Practice, 2d. Ed., Marcel Dekker, NY, NY, 1995, pp. 379-80.
  • water and heat accelerate the decomposition of some compounds.
  • the effect of water on a fonnulation can be of great significance since moisture and/or humidity are commonly encountered during manufacture, handling, packaging, storage, shipment, and use of formulations.
  • Anhydrous pharmaceutical compositions and dosage forms ofthe invention can be prepared using anhydrous or low moisture containing ingredients and low moisture or low humidity conditions.
  • Pharmaceutical compositions and dosage forms that comprise lactose and at least one active ingredient that comprises a primary or secondary amine are preferably anhydrous if substantial contact with moisture and/or humidity during manufacturing, packaging, and/or storage is expected.
  • anhydrous pharmaceutical composition should be prepared and stored such that its anhydrous nature is maintained. Accordingly, anhydrous compositions are preferably packaged using materials known to prevent exposure to water such that they can be included in suitable formulary kits. Examples of suitable packaging include, but are not limited to, hermetically sealed foils, plastics, unit dose containers (e.g., vials), blister packs, and strip packs.
  • suitable packaging include, but are not limited to, hermetically sealed foils, plastics, unit dose containers (e.g., vials), blister packs, and strip packs.
  • suitable packaging include, but are not limited to, hermetically sealed foils, plastics, unit dose containers (e.g., vials), blister packs, and strip packs.
  • suitable packaging include, but are not limited to, hermetically sealed foils, plastics, unit dose containers (e.g., vials), blister packs, and strip packs.
  • the invention further encompasses pharmaceutical compositions and dosage forms that comprise one or more compounds that reduce the rate by which an active ingredient will decompose
  • the amounts and specific types of active ingredients in a dosage form may differ depending on factors such as, but not limited to, the route by which it is to be administered to patients.
  • typical dosage forms ofthe invention comprise immunomodulatory compounds or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, in an amount of from about 0.10 to about 150 mg.
  • Typical dosage forms comprise immunomodulatory compounds or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, clathrate, or prodrug thereof, in an amount of about 0.1, 1, 2, 5, 7.5, 10, 12.5, 15, 17.5, 20, 25, 50, 100, 150 or 200 mg.
  • a preferred dosage form comprises 4-(amino)-2-(2,6- dioxo(3-piperidyl))-isoindoline-l,3-dione (ActimidTM) in an amount of about 1, 2, 5, 10, 25 or 50 mg.
  • ActimidTM 4-(amino)-2-(2,6- dioxo(3-piperidyl))-isoindoline-l,3-dione
  • ActimidTM 4-(amino)-2-(2,6- dioxo(3-piperidyl))-isoindoline-l,3-dione
  • ActimidTM 4-(amino)-2-(2,6- dioxo(3-piperidyl))-isoindoline-l,3-dione
  • ActimidTM 4-(amino)-2-(2,6- dioxo(3-piperidyl))-isoindoline-l,3-dione
  • Typical dosage forms comprise the second active agent in an amount of form about 1 to about 3,500 mg, from about 5 to about 2,500 mg, from about 10 to about 500 mg, or from about 25 to about 250 mg.
  • the specific amount ofthe second active agent will depend on the specific agent used, the type of pain being treated or managed, and the amount(s) of immunomodulatory compounds and any optional additional active agents concurrently administered to the patient.
  • compositions ofthe invention that are suitable for oral administration can be presented as discrete dosage forms, such as, but are not limited to, tablets (e.g., chewable tablets), caplets, capsules, and liquids (e.g., flavored syrups).
  • dosage forms contain predetermined amounts of active agents, and may be prepared by methods of pharmacy well known to those skilled in the art. See generally, Remington 's Pharmaceutical Sciences, 18th ed., Mack Publishing, Easton PA (1990).
  • Typical oral dosage forms ofthe invention are prepared by combining the active ingredients in an intimate admixture with at least one excipient according to conventional pharmaceutical compounding techniques. Excipients can take a wide variety of forms depending on the form of preparation desired for administration.
  • excipients suitable for use in oral liquid or aerosol dosage forms include, but are not limited to, water, glycols, oils, alcohols, flavoring agents, preservatives, and coloring agents.
  • excipients suitable for use in solid oral dosage forms include, but are not limited to, starches, sugars, micro-crystalline cellulose, diluents, granulating agents, lubricants, binders, and disintegrating agents.
  • tablets and capsules represent the most advantageous oral dosage unit forms, in which case solid excipients are employed. If desired, tablets can be coated by standard aqueous or nonaqueous techniques. Such dosage forms can be prepared by any ofthe methods of pharmacy. In general, pharmaceutical compositions and dosage forms are prepared by uniformly and intimately admixing the active ingredients with liquid carriers, finely divided solid carriers, or both, and then shaping the product into the desired presentation if necessary.
  • a tablet can be prepared by compression or molding.
  • Compressed tablets can be prepared by compressing in a suitable machine the active ingredients in a free-flowing form such as powder or granules, optionally mixed with an excipient.
  • Molded tablets can be made by molding in a suitable machine a mixture ofthe powdered compound moistened with an inert liquid diluent.
  • excipients that can be used in oral dosage forms ofthe invention include, but are not limited to, binders, fillers, disintegrants, and lubricants.
  • Binders suitable for use in pharmaceutical compositions and dosage forms include, but are not limited to, corn starch, potato starch, or other starches, gelatin, natural and synthetic gums such as acacia, sodium alginate, alginic acid, other alginates, powdered tragacanth, guar gum, cellulose and its derivatives (e.g., ethyl cellulose, cellulose acetate, carboxymethyl cellulose calcium, sodium carboxymethyl cellulose), polyvinyl pyrrolidone, methyl cellulose, pre-gelatinized starch, hydroxypropyl methyl cellulose, (e.g., Nos. 2208, 2906, 2910), macrocrystalline cellulose, and mixtures thereof.
  • Suitable forms of microcrystalline cellulose include, but are not limited to, the materials sold as AVICEL-PH-101, AVICEL-PH-103 AVICEL RC-581, AVICEL-PH-105 (available from FMC Corporation, American Viscose Division, Avicel Sales, Marcus Hook, PA), and mixtures thereof.
  • An specific binder is a mixture of microcrystalline cellulose and sodium carboxymethyl cellulose sold as AVICEL RC-581.
  • Suitable anhydrous or low moisture excipients or additives include AVICEL-PH-103TM and Starch 1500 LM.
  • fillers suitable for use in the pharmaceutical compositions and dosage forms disclosed herein include, but are not limited to, talc, calcium carbonate (e.g., granules or powder), microcrystalline cellulose, powdered cellulose, dextrates, kaolin, mannitol, silicic acid, sorbitol, starch, pre-gelatinized starch, and mixtures thereof.
  • the binder or filler in pharmaceutical compositions ofthe invention is typically present in from about 50 to about 99 weight percent ofthe pharmaceutical composition or dosage form.
  • Disintegrants are used in the compositions ofthe invention to provide tablets that disintegrate when exposed to an aqueous environment. Tablets that contain too much disintegrant may disintegrate in storage, while those that contain too little may not disintegrate at a desired rate or under the desired conditions. Thus, a sufficient amount of disintegrant that is neither too much nor too little to detrimentally alter the release ofthe active ingredients should be used to form solid oral dosage forms ofthe invention.
  • the amount of disintegrant used varies based upon the type of formulation, and is readily discernible to those of ordinary skill in the art.
  • Typical pharmaceutical compositions comprise from about 0.5 to about 15 weight percent of disintegrant, preferably from about 1 to about 5 weight percent of disintegrant.
  • Disintegrants that can be used in pharmaceutical compositions and dosage forms of the invention include, but are not limited to, agar-agar, alginic acid, calcium carbonate, microcrystalline cellulose, croscarmellose sodium, crospovidone, polacrilin potassium, sodium starch glycolate, potato or tapioca starch, other starches, pre-gelatinized starch, other starches, clays, other algins, other celluloses, gums, and mixtures thereof.
  • Lubricants that can be used in pharmaceutical compositions and dosage forms ofthe invention include, but are not limited to, calcium stearate, magnesium stearate, mineral oil, light mineral oil, glycerin, sorbitol, mannitol, polyethylene glycol, other glycols, stearic acid, sodium lauryl sulfate, talc, hydrogenated vegetable oil (e.g., peanut oil, cottonseed oil, sunflower oil, sesame oil, olive oil, corn oil, and soybean oil), zinc stearate, ethyl oleate, ethyl laureate, agar, and mixtures thereof.
  • calcium stearate e.g., magnesium stearate, mineral oil, light mineral oil, glycerin, sorbitol, mannitol, polyethylene glycol, other glycols, stearic acid, sodium lauryl sulfate, talc
  • hydrogenated vegetable oil e.g., peanut oil, cottonseed oil
  • Additional lubricants include, for example, a syloid silica gel (AEROSLL200, manufactured by W.R. Grace Co. of Baltimore, MD), a coagulated aerosol of synthetic silica (marketed by Degussa Co. of Piano, TX), CAB-O-SIL (a pyrogenic silicon dioxide product sold by Cabot Co. of Boston, MA), and mixtures thereof. If used at all, lubricants are typically used in an amount of less than about 1 weight percent ofthe pharmaceutical compositions or dosage forms into which they are incorporated.
  • AEROSLL200 a syloid silica gel
  • a coagulated aerosol of synthetic silica marketed by Degussa Co. of Piano, TX
  • CAB-O-SIL a pyrogenic silicon dioxide product sold by Cabot Co. of Boston, MA
  • lubricants are typically used in an amount of less than about 1 weight percent ofthe pharmaceutical compositions or dosage forms into which they are incorporated.
  • a preferred solid oral dosage form ofthe invention comprises immunomodulatory compounds, anhydrous lactose, microcrystalline cellulose, polyvinylpyrrolidone, stearic acid, colloidal anhydrous silica, and gelatin. 4.4.2 Delayed Release Dosage Forms
  • Active agents ofthe invention can be administered by controlled release means or by delivery devices that are well known to those of ordinary skill in the art. Examples include, but are not limited to, those described in U.S. Patent Nos.: 3,845,770; 3,916,899; 3,536,809; 3,598,123; and 4,008,719, 5,674,533, 5,059,595, 5,591,767, 5,120,548, 5,073,543, 5,639,476, 5,354,556, and 5,733,566, each of which is incorporated herein by reference.
  • Such dosage forms can be used to provide slow or controlled-release of one or more active ingredients using, for example, hydropropylmethyl cellulose, other polymer matrices, gels, permeable membranes, osmotic systems, multilayer coatings, microparticles, liposomes, microspheres, or a combination thereof to provide the desired release profile in varying proportions.
  • Suitable controlled-release formulations known to those of ordinary skill in the art, including those described herein, can be readily selected for use with the active ingredients ofthe invention.
  • the invention thus encompasses single unit dosage forms suitable for oral administration such as, but not limited to, tablets, capsules, gelcaps, and caplets that are adapted for controlled-release.
  • controlled-release pharmaceutical products have a common goal of improving drug therapy over that achieved by their non-controlled counterparts.
  • the use of an optimally designed controlled-release preparation in medical treatment is characterized by a minimum of drug substance being employed to cure or control the condition in a minimum amount of time.
  • Advantages of controlled-release formulations include extended activity of the drug, reduced dosage frequency, and increased patient compliance, fn addition, controlled-release formulations can be used to affect the time of onset of action or other characteristics, such as blood levels ofthe drug, and can thus affect the occurrence of side (e.g., adverse) effects.
  • Controlled-release formulations are designed to initially release an amount of drug (active ingredient) that promptly produces the desired therapeutic effect, and gradually and continually release of other amounts of drug to maintain this level of therapeutic or prophylactic effect over an extended period of time.
  • the drug In order to maintain this constant level of drug in the body, the drug must be released from the dosage form at a rate that will replace the amount of drug being metabolized and excreted from the body.
  • Controlled- release of an active ingredient can be stimulated by various conditions including, but not limited to, pH, temperature, enzymes, water, or other physiological conditions or compounds.
  • Parenteral dosage forms can be administered to patients by various routes including, but not limited to, subcutaneous, intravenous (including bolus injection), intramuscular, and intraarterial. Because their administration typically bypasses patients' natural defenses against contaminants, parenteral dosage forms are preferably sterile or capable of being sterilized prior to administration to a patient. Examples of parenteral dosage forms include, but are not limited to, solutions ready for injection, dry products ready to be dissolved or suspended in a pharmaceutically acceptable vehicle for injection, suspensions ready for injection, and emulsions.
  • Suitable vehicles that can be used to provide parenteral dosage forms ofthe invention are well known to those skilled in the art. Examples include, but are not limited to: Water for Injection USP; aqueous vehicles such as, but not limited to, Sodium Chloride Injection, Ringer's Injection, Dextrose Injection, Dextrose and Sodium Chloride Injection, and Lactated Ringer's Injection; water-miscible vehicles such as, but not limited to, ethyl alcohol, polyethylene glycol, and polypropylene glycol; and non-aqueous vehicles such as, but not limited to, com oil, cottonseed oil, peanut oil, sesame oil, ethyl oleate, isopropyl myristate, and benzyl benzoate.
  • water for Injection USP Water for Injection USP
  • aqueous vehicles such as, but not limited to, Sodium Chloride Injection, Ringer's Injection, Dextrose Injection, Dextrose and Sodium Chlor
  • cyclodextrin and its derivatives can be used to increase the solubility of immunomodulatory compounds and its derivatives. See, e.g., U.S. Patent No. 5,134,127, which is incorporated herein by reference.
  • Topical and mucosal dosage forms ofthe invention include, but are not limited to, sprays, aerosols, solutions, emulsions, suspensions, or other forms known to one of skill in the art. See, e.g., Remington 's Pharmaceutical Sciences, 16 (1 and 18* eds., Mack
  • Dosage forms suitable for treating mucosal tissues within the oral cavity can be formulated as mouthwashes or as oral gels.
  • Suitable excipients e.g., carriers and diluents
  • other materials that can be used to provide topical and mucosal dosage forms encompassed by this invention are well known to those skilled in the pharmaceutical arts, and depend on the particular tissue to which a given pharmaceutical composition or dosage form will be applied.
  • typical excipients include, but are not limited to, water, acetone, ethanol, ethylene glycol, propylene glycol, butane- 1, 3 -diol, isopropyl myristate, isopropyl palmitate, mineral oil, and mixtures thereof to form solutions, emulsions or gels, which are non-toxic and pharmaceutically acceptable.
  • Moisturizers or humectants can also be added to pharmaceutical compositions and dosage forms if desired.
  • additional ingredients are well known in the art. See, e.g., Remington 's Pharmaceutical Sciences, 16 th and 18 th eds., Mack Publishing, Easton PA (1980 & 1990).
  • the pH of a pharmaceutical composition or dosage form may also be adjusted to improve delivery of one or more active ingredients.
  • the polarity of a solvent carrier, its ionic strength, or tonicity can be adjusted to improve delivery.
  • Compounds such as stearates can also be added to pharmaceutical compositions or dosage forms to advantageously alter the hydrophilicity or lipophilicity of one or more active ingredients so as to improve delivery.
  • stearates can serve as a lipid vehicle for the formulation, as an emulsifying agent or surfactant, and as a delivery-enhancing or penetration-enhancing agent.
  • Different salts, hydrates or solvates ofthe active ingredients can be used to further adjust the properties ofthe resulting composition. 4.4.5 Kits
  • kits which, when used by the medical practitioner, can simplify the administration of appropriate amounts of active ingredients to a patient.
  • a typical kit of the invention comprises a dosage form of immunomodulatory compounds, or a pharmaceutically acceptable salt, solvate, hydrate, stereoisomer, prodrug, or clathrate thereof. Kits encompassed by this invention can further comprise additional active ingredients or a combination thereof.
  • additional active ingredients include, but are not limited to, antidepressants, anticonvulsants, antihypertensives, anxiolytics, calcium channel blockers, muscle relaxants, non-narcotic analgesics, opioid analgesics, anti-inflammatories, cox-2 inhibitors, immunomodulatory agents, immunosuppressive agents, corticosteroids, hyperbaric oxygen, or other therapeutics discussed herein (see, e.g., section 4.2).
  • Kits ofthe invention can further comprise devices that are used to administer the active ingredients.
  • devices include, but are not limited to, syringes, drip bags, patches, and inhalers.
  • Kits ofthe invention can further comprise pharmaceutically acceptable vehicles that can be used to administer one or more active ingredients.
  • the kit can comprise a sealed container of a suitable vehicle in which the active ingredient can be dissolved to form a particulate-free sterile solution that is suitable for parenteral administration.
  • Examples of pharmaceutically acceptable vehicles include, but are not limited to: Water for Injection USP; aqueous vehicles such as, but not limited to, Sodium Chloride Injection, Ringer's Injection, Dextrose Injection, Dextrose and Sodium Chloride Injection, and Lactated Ringer's Injection; water-miscible vehicles such as, but not limited to, ethyl alcohol, polyethylene glycol, and polypropylene glycol; and non-aqueous vehicles such as, but not limited to, com oil, cottonseed oil, peanut oil, sesame oil, ethyl oleate, isopropyl myristate, and benzyl benzoate. 5.
  • Water for Injection USP Water for Injection USP
  • aqueous vehicles such as, but not limited to, Sodium Chloride Injection, Ringer's Injection, Dextrose Injection, Dextrose and Sodium Chloride Injection, and Lactated Ringer's Injection
  • TNF- ⁇ may play a pathological role in both nociceptive pain and neuropathic pain.
  • immunomodulatory compounds enhance the degradation of TNF- ⁇ mRNA. Increase of its expression in Schwann cells is shown in human painful neuropathies. Soluble TNF- ⁇ receptors are increased in the serum of patients with allodynia, as compared with neuropathy patients who do not report allodynia.
  • the cytokine can induce ectopic activity in primary afferent nociceptors, and thus is a potential cause of hyperalgesia in neuropathic pain.
  • TNF-o can form active sodium ion channels in cells. Increased influx of sodium into nociceptors would dispose them toward ectopic discharge.
  • the cytokine may play a pathological role if it is active at sites of nerve damage or dysfunction.
  • immunomodulatory compounds when used pre-emptively, may reduce mechanical allodynia and thermal hyperalgesia in rats subjected to the chronic constriction injury model of neuropathic pain, hi addition to reducing endoneurial TNF- ⁇ , the compounds may also cause a long-term increase in spinal cord dorsal horn met-enkephalin, an important antinociceptive neurotransmitter. Immunomodulatory compounds may also inhibit inflammatory hyperalgesia in rats and the writhing nociceptive response in mice.
  • the IC 5 o's of 3-(4-amino-l-oxo-l,3-dihydro-isoindol-2-yl)-piperidine-2,6-dione for inhibiting production of TNF- ⁇ following LPS-stimulation of PBMC and human whole blood were -100 nM (25.9 ng/mL) and -480 nM (103.6 ng/mL), respectively.
  • Thahdomide in contrast, had an IC 50 of -194 ⁇ M (50.1 j g/mL) for inhibiting production of TNF-o. following LPS-stimulation of PBMC.
  • IC 50 -194 ⁇ M (50.1 j g/mL) for inhibiting production of TNF-o. following LPS-stimulation of PBMC.
  • 3-(4-amino-l-oxo-l,3 -dihydro-isoindol-2-yl)-piperidine-2,6-dione or 4-(amino)-2-(2,6-dioxo-(3-piperidyl))- isoindoline-l,3-dione is similar to, but 50 to 2,000 times more potent than, thahdomide.
  • 3-(4-amino-l-oxo-l,3 -dihydro-isoindol-2-yl)-piperidine-2,6-dione or 4-(amino)-2-(2,6-dioxo-(3-piperidyl))- isoindoline-l,3-dione suppresses the generation of inflammatory cytokines, down-regulates adhesion molecules and apoptosis inhibitory proteins (e.g., cFLJJP, cIAP), promotes sensitivity to death-receptor initiated programmed cell death, and suppresses angiogenic response.
  • apoptosis inhibitory proteins e.g., cFLJJP, cIAP
  • 3-(4-amino-l-oxo-l,3-dihydro-isoindol-2-yl) -piperidine-2,6-dione or 4-(amino)-2-(2,6-dioxo-(3-piperidyl))-isoindoline-l,3-dione is approximately 50 to 100 times more potent than thahdomide in stimulating the proliferation of T-cells following primary induction by T-cell receptor (TCR) activation.
  • the compounds are also approximately 50 to 100 times more potent than thahdomide in augmenting the production of IL2 and IFN-7 following TCR activation of PBMC (IL2) or T-cells (IFN-7).
  • the compounds exhibited dose-dependent inhibition of LPS- stimulated production ofthe pro-inflammatory cytokines TNF-o, ILl ⁇ and IL6 by PBMC while they increased production ofthe anti-inflammatory cytokine IL10.
  • One group received three doses of vehicle only and the other receives three ascending doses of 3-(4-amino-l-oxo-l,3-dihydro-isoindol-2-yl)-piperidine- 2,6-dione or 4-(amino)-2-(2,6-dioxo-(3-piperidyl))-isoindoline-l,3-dione (2, 10, and 20 mg/kg).
  • Immunomodulatory compounds can be tested for their ability to treat, prevent, manage and/or modify pain using any pain models well-known in the art.
  • a variety of animal pain models are described in Hogan, Q., Regional Anesthesia and Pain Medicine 27 (4) -.385-401 (2002), which is incorporated by reference herein in its entirety.
  • nociceptive pain models include a formalin test, hot-plate test and tail- flick test.
  • formalin test hot-plate test
  • tail-flick test Illustrative examples ofthe formalin test, hot-plate test and tail-flick test are set forth below.
  • the most commonly used neuropathic pain models are the Bennett, Selzer, and Chung models. Siddall, P.J. and Munglani, R., Animal Models of Pain, pp 377-384 in Bountra, C, Munglani, R., Schmidt, W.K., eds. Pain: Current Understanding, Emerging Therapies and Novel Approaches to Drug Discovery, Marcel Dekker, Inc., New York, 2003.
  • the Bennett and Selzer models are well-known and rapid to perform.
  • the Chung model is robust for mechanical allodynia in most animals and is well characterized though complicated. These models represent a range of approaches to try and mimic some ofthe damage and dysfunction in clinical conditions.
  • diseases associated with pain such as diabetic neuropathy or the new bone cancer and visceral pain models
  • Animals are injected with an immunomodulatory compound or vehicle (controls) followed by the injection of formalin into the dorsal surface ofthe paw. The animal is observed to determine the number of times it flinches the injected paw over a period of 60 minutes. This model allows for the evaluation of anti-nociceptive drugs in the treatment of pain. Abbott, F. et al. Pain 60:91-102 (1995). Animals are contained in shoe box cages for the duration ofthe experiment. Formalin (50 ⁇ l; 0.5%) is injected into the dorsal surface ofthe rear, right paw, by placing the needle (28.5G) above the toes and below the ankle and inserting it beneath the surface of the skin. A timer is started immediately after the injection to mark the beginning of phase 1.
  • phase 2 begins. Flinches are counted as in phase 1 for the next 20 minutes.
  • An immunomodulatory compound is administered in an amount of from about 0.10 to about 150 mg/day by oral route up to 24 hrs prior to the formalin test. Animals are repeated in the order they are treated. Immediately following the completion ofthe test periods, animals are euthanized by CO 2 asphyxiation in accordance with IACUC guidelines.
  • Morphine treatment is used to determine the optimal hotplate temperature. Doses of 8 to 10 mg/kg morphine (i.p.) provide a near-maximal anti-nociceptive response in acute pain assays. The apparatus is set to the temperature at which this type of anti-nociceptive response is observed with these doses of morphine (approximately 55°C). An immunomodulatory compound is administered in an amount of from about 0.10 to about 150 mg/day by oral route up to 24 hrs prior to the hot-plate test. When the post-treatment time is elapsed, individual testing of animals is begun. A single animal is placed on the hot plate and a stopwatch or timer is immediately started.
  • the animal is observed until it shows a nociceptive response (e.g., licks its paw) or until the cut-off time of 30 seconds is reached (to minimize tissue damage that can occur with prolonged exposure to a heated surface).
  • the animal is removed from the hot-plate and its latency time to respond is recorded. For animals that do not respond prior to the cut-off time, the cut-off time will be recorded as their response time. Animals are repeated in the order they are treated. Animals are euthanized immediately following the experiment by CO 2 asphyxiation in accordance with IACUC guidelines. Any animal experiencing unanticipated events at any time point throughout this study is evaluated for veterinary intervention. Any animal that cannot recover with standard veterinary care is euthanized immediately by CO 2 asphyxiation in accordance with IACUC guidelines.
  • An immunomodulatory compound is administered in an amount of from about 0.10 to about 150 mg/day by oral route up to 24 hrs prior to the tail flick test in accordance with the IACUC guidelines.
  • post-treatment time is elapsed, individual testing of animals is begun.
  • a single animal is placed on a tail flick apparatus exposing the ventral tail surface to a focused light beam.
  • Response latency is the time from the application of the light until the tail is flicked. The animal is observed until it shows a nociceptive response (e.g., tail flick) or until the cut-off time of 10 seconds is reached (to minimize tissue damage that can occur with prolonged exposure to a heated surface).
  • the animal is removed from the light source, its latency time to respond is recorded and then the animal is euthanized immediately by CO 2 asphyxiation in accordance with IACUC guidelines.
  • the light beam intensity is adjusted to produce a baseline latency of 2.5-4 seconds.
  • the cut-off time is recorded as their response time. Animals are repeated in the order they are treated.
  • a model particularly useful for thermal allodynia is the topical capsaicin-induced thermal allodynia model. Butelman, E.R. et al, J. of Pharmacol. Exp. Therap. 306:1106- 1114 (2003). This model is a modification ofthe warm water tail withdrawal model. Ko, M.C. et al, J. of Pharmacol. Exp. Therap. 289:318-385 (1999). Briefly, monkeys sit in a custom made chair in a temperature-controlled room (20-22°C). Their tails are shaved with standard clippers and tail withdrawal latencies are timed in 0.1 second increments up to a maximum of 20 seconds in both 38°C and 42°C water stimuli to provide a baseline.
  • capsaicin is dissolved in a vehicle composed of 70% ethanol and 30% sterile water for a final capsaicin concentration of either 0.0013 or 0.004 M.
  • the solution (0.3 mL) is slowly injected onto a gauze patch, saturating the patch and avoiding overflow.
  • capsaicin patch is fastened to the tail with tape.
  • the patch is removed and tail withdrawal testing in both 38°C and 42°C water stimuli is performed as described above. Allodynia is detected as a decrease in tail withdrawal latency compared to the baseline measurements.
  • a single dose ofthe compound is administered prior to (e.g., 15 minutes prior, 30 minutes prior, 60 minutes prior or 90 minutes prior) the application ofthe capsaicin patch.
  • the allodynia reversal properties of an immunomodulatory compound can be determined by administering a single dose ofthe compound after application ofthe capsaicin patch (e.g., immediately after, 30 minutes after, 60 minutes after or 90 minutes after).
  • the capsaicin model may be appropriate for agents to be used to treat hyperalgesia and allodynia (e.g. vanilloid receptor 1 (VR1) antagonists and AMPA antagonists), whereas UV skin bum may be appropriate for bradykinin Bl receptor antagonists, cannabinoid agonists, and VR1 antagonists.
  • VR1 vanilloid receptor 1
  • Clinical applications ofthe capsaicin model have supported the antihyperalgesic effects of several clinically used drugs such as opioids, local anesthetics, ketamine and gabapentin.
  • Visceral models have, as yet, unknown potential as hyperalgesic models and require validation.
  • Immunomodulatory compounds such as 4-(amino)-2-(2,6-dioxo(3-piperidyl))- isoindoline-l,3-dione and 3-(4-amino-l-oxo-l,3-dihydro-isoindol-2-yl)-piperidine- 2,6-dione are administered in an amount of 0.1 to 25 mg per day to patients with pain syndromes for three to six months.
  • a baseline evaluation is performed for the effect ofthe drug treatment on pain intensity, impact of pain on activities of daily living, and consumption of other pain medications.
  • clinical studies are performed in pain patients who have upper extremity CRPS that has not responded to conventional physical therapy and has been present for at least one year.
  • VAS Numeric Pain Scale Assessment

Landscapes

  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pain & Pain Management (AREA)
  • Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne des méthodes permettant de traiter, de prévenir, de modifier et de soulager divers types de douleur. Des méthodes spécifiques consistent, d'une part, à administrer un composé immunomodulateur ou un sel, solvate, hydrate, stéréo-isomère, clathrate ou promédicament pharmaceutiquement acceptable de ce composé, seul ou combiné avec un second agent actif, et/ou, d'autre part, à conduire une thérapie chirurgicale, psychologique ou physique. L'invention concerne également des compositions pharmaceutiques, des formes posologiques monodoses et des trousses convenant pour une utilisation dans les méthodes selon l'invention.
EP03777871A 2002-10-24 2003-10-24 Compositions contenant des composes immunomodulateurs pour le traitement, la modification et le soulagement de la douleur et leurs methodes d'utilisation Withdrawn EP1556044A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42100302P 2002-10-24 2002-10-24
US421003P 2002-10-24
PCT/US2003/033757 WO2004037199A2 (fr) 2002-10-24 2003-10-24 Compositions contenant des composes immunomodulateurs pour le traitement, la modification et le soulagement de la douleur et leurs methodes d'utilisation

Publications (1)

Publication Number Publication Date
EP1556044A2 true EP1556044A2 (fr) 2005-07-27

Family

ID=32176661

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03777871A Withdrawn EP1556044A2 (fr) 2002-10-24 2003-10-24 Compositions contenant des composes immunomodulateurs pour le traitement, la modification et le soulagement de la douleur et leurs methodes d'utilisation

Country Status (13)

Country Link
EP (1) EP1556044A2 (fr)
JP (1) JP2006507284A (fr)
KR (1) KR20050057672A (fr)
CN (2) CN101108185A (fr)
AU (1) AU2003286663B2 (fr)
BR (1) BR0315609A (fr)
CA (1) CA2503536A1 (fr)
HK (1) HK1088225A1 (fr)
MX (1) MXPA05004182A (fr)
NZ (1) NZ540028A (fr)
TW (1) TW200503683A (fr)
WO (1) WO2004037199A2 (fr)
ZA (1) ZA200503240B (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU228769B1 (en) 1996-07-24 2013-05-28 Celgene Corp Substituted 2(2,6-dioxopiperidin-3-yl)phthalimides and -1-oxoisoindolines and their use for production of pharmaceutical compositions for mammals to reduce the level of tnf-alpha
US20050203142A1 (en) * 2002-10-24 2005-09-15 Zeldis Jerome B. Methods of using and compositions comprising immunomodulatory compounds for treatment, modification and management of pain
US7563810B2 (en) * 2002-11-06 2009-07-21 Celgene Corporation Methods of using 3-(4-amino-1-oxo-1,3-dihydroisoindol-2-yl)-piperidine-2,6-dione for the treatment and management of myeloproliferative diseases
US20050143344A1 (en) * 2003-12-30 2005-06-30 Zeldis Jerome B. Methods and compositions using immunomodulatory compounds for the treatment and management of central nervous system disorders or diseases
US20050222209A1 (en) * 2004-04-01 2005-10-06 Zeldis Jerome B Methods and compositions for the treatment, prevention or management of dysfunctional sleep and dysfunctional sleep associated with disease
US20080300262A1 (en) * 2005-04-08 2008-12-04 Snutch Terrance P Combination Therapy for Relief of Pain
US20070066512A1 (en) * 2005-09-12 2007-03-22 Dominique Verhelle Methods and compositions using immunomodulatory compounds for the treatment of disorders associated with low plasma leptin levels
JP4382735B2 (ja) * 2005-10-06 2009-12-16 独立行政法人科学技術振興機構 神経因性疼痛治療剤
JP2009538318A (ja) * 2006-05-26 2009-11-05 セルジーン・コーポレーション 併用療法において免疫調節化合物を用いる方法及び組成物
WO2008057196A2 (fr) * 2006-10-19 2008-05-15 Celgene Corporation Procédés et compositions utilisant des composés immunomodulateurs pour le traitement et la gestion de la spirochète et autres maladies à bactéries intracelulaires obligées
CN101696205B (zh) 2009-11-02 2011-10-19 南京卡文迪许生物工程技术有限公司 3-(取代二氢异吲哚-2-基)-2,6-哌啶二酮多晶型物和药用组合物

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4215114A (en) * 1976-11-12 1980-07-29 The Upjohn Company Analgesic N-[2-(furyl-methylamino and 2-thienylmethylamino)cycloaliphatic]be
US6281230B1 (en) * 1996-07-24 2001-08-28 Celgene Corporation Isoindolines, method of use, and pharmaceutical compositions
HU228769B1 (en) * 1996-07-24 2013-05-28 Celgene Corp Substituted 2(2,6-dioxopiperidin-3-yl)phthalimides and -1-oxoisoindolines and their use for production of pharmaceutical compositions for mammals to reduce the level of tnf-alpha
PT2177517E (pt) * 1996-07-24 2011-11-10 Celgene Corp 2-(2,6-dioxopiperidin-3-il)-ftalimida amino-substituída para redução dos níveis de tnf-alfa
US5955476A (en) * 1997-11-18 1999-09-21 Celgene Corporation Substituted 2-(2,6-dioxo-3-fluoropiperidin-3-yl)-isoindolines and method of reducing inflammatory cytokine levels
US20030045552A1 (en) * 2000-12-27 2003-03-06 Robarge Michael J. Isoindole-imide compounds, compositions, and uses thereof
SE0101256D0 (sv) * 2001-04-06 2001-04-06 A & Science Invest Ab Treatment of low back pain

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2004037199A2 *

Also Published As

Publication number Publication date
AU2003286663A1 (en) 2004-05-13
MXPA05004182A (es) 2005-06-08
HK1088225A1 (en) 2006-11-03
CN1732000A (zh) 2006-02-08
AU2003286663B2 (en) 2009-08-13
CA2503536A1 (fr) 2004-05-06
KR20050057672A (ko) 2005-06-16
JP2006507284A (ja) 2006-03-02
NZ540028A (en) 2009-07-31
CN101108185A (zh) 2008-01-23
WO2004037199A2 (fr) 2004-05-06
CN1326522C (zh) 2007-07-18
TW200503683A (en) 2005-02-01
BR0315609A (pt) 2005-08-23
WO2004037199A3 (fr) 2004-12-23
ZA200503240B (en) 2007-11-28

Similar Documents

Publication Publication Date Title
US20050203142A1 (en) Methods of using and compositions comprising immunomodulatory compounds for treatment, modification and management of pain
AU2003284979B2 (en) Methods of using and compositions comprising selective cytokine inhibitory drugs for treatment, modification and management of pain
AU2005304420A1 (en) Methods and compositions using immunomodulatory compounds for treatment and management of parasitic diseases
AU2003286663B2 (en) Methods of using and compositions comprising immunomodulatory compounds for treatment, modification and management of pain
US7612096B2 (en) Methods for treatment, modification and management of radiculopathy using 1-oxo-2-(2,6-dioxopiperidin-3yl)-4-aminoisoindoline
US20070161696A1 (en) Methods of using and compositions comprising selective cytokine inhibitory drugs for treatment, modification and management of pain
JP2007509170A (ja) 線維筋痛症の治療のためのサリドマイドを含んでなる方法および組成物
CA2543132A1 (fr) Procedes d'utilisations et compositions comprenant des medicaments selectifs inhibiteurs de la cytokine utilises dans le traitement, la modification et la gestion de la douleur

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20050428

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK

DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CELGENE CORPORATION

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20100504