EP1532241A4 - Rationell konstruierte, von chondroitinase b abgeleitete lyasen - Google Patents

Rationell konstruierte, von chondroitinase b abgeleitete lyasen

Info

Publication number
EP1532241A4
EP1532241A4 EP03756399A EP03756399A EP1532241A4 EP 1532241 A4 EP1532241 A4 EP 1532241A4 EP 03756399 A EP03756399 A EP 03756399A EP 03756399 A EP03756399 A EP 03756399A EP 1532241 A4 EP1532241 A4 EP 1532241A4
Authority
EP
European Patent Office
Prior art keywords
chondroitinase
lyases
rationalally
designed
inhibiting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP03756399A
Other languages
English (en)
French (fr)
Other versions
EP1532241B1 (de
EP1532241A2 (de
Inventor
Kevin Pojasek
Rahul Raman
Ram Sasisekharan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of EP1532241A2 publication Critical patent/EP1532241A2/de
Publication of EP1532241A4 publication Critical patent/EP1532241A4/de
Application granted granted Critical
Publication of EP1532241B1 publication Critical patent/EP1532241B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Dermatology (AREA)
  • Materials Engineering (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Confectionery (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
EP03756399A 2002-06-03 2003-06-03 Rationell konstruierte, von chondroitinase b abgeleitete lyasen Expired - Lifetime EP1532241B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38550902P 2002-06-03 2002-06-03
US385509P 2002-06-03
PCT/US2003/017680 WO2003102160A2 (en) 2002-06-03 2003-06-03 Rationally designed polysaccharide lyases derived from chondroitinase b

Publications (3)

Publication Number Publication Date
EP1532241A2 EP1532241A2 (de) 2005-05-25
EP1532241A4 true EP1532241A4 (de) 2005-12-14
EP1532241B1 EP1532241B1 (de) 2010-09-15

Family

ID=29712183

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03756399A Expired - Lifetime EP1532241B1 (de) 2002-06-03 2003-06-03 Rationell konstruierte, von chondroitinase b abgeleitete lyasen

Country Status (7)

Country Link
US (4) US6962699B2 (de)
EP (1) EP1532241B1 (de)
AT (1) ATE481478T1 (de)
AU (1) AU2003243396A1 (de)
CA (1) CA2493509C (de)
DE (1) DE60334220D1 (de)
WO (1) WO2003102160A2 (de)

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WO2001066772A2 (en) * 2000-03-08 2001-09-13 Massachusetts Institute Of Technology Heparinase iii and uses thereof
ATE426805T1 (de) * 2000-09-12 2009-04-15 Massachusetts Inst Technology Verfahren und produkte, die mit niedermolekularem heparin assoziiert sind
AU2440802A (en) 2000-10-18 2002-04-29 Massachusetts Inst Technology Methods and products related to pulmonary delivery of polysaccharides
ES2421134T3 (es) 2002-05-03 2013-08-29 Massachusetts Institute Of Technology delta 4,5 Glicuronidasa y usos de la misma
US8183350B2 (en) 2002-05-04 2012-05-22 Acorda Therapeutics, Inc. Compositions and methods for promoting neuronal outgrowth
AU2003302219B8 (en) * 2002-05-20 2009-03-26 Instituto Di Recherche Chimiche E Biochimiche G. Ronzoni Novel method for sequence determination using NMR
CA2493509C (en) * 2002-06-03 2010-03-09 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from chondroitinase b
AU2003265561A1 (en) * 2002-08-15 2004-03-03 Acorda Therapeutics, Inc. Chimeric protein
JP4606712B2 (ja) * 2003-01-08 2011-01-05 マサチューセッツ インスティテュート オブ テクノロジー ２−ｏスルファターゼ組成物および関連の方法
EP3211084B1 (de) * 2003-05-16 2019-03-27 Acorda Therapeutics, Inc. Proteoglycan-abbauende mutanten zur behandlung des zns
US7959914B2 (en) 2003-05-16 2011-06-14 Acorda Therapeutics, Inc. Methods of reducing extravasation of inflammatory cells
AU2016204464B2 (en) * 2003-05-16 2018-03-22 Acorda Therapeutics, Inc. Proteoglycan degrading mutants for treatment of cns
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US20070161599A1 (en) * 2003-05-28 2007-07-12 The Board Of Trustees Of The University Of Illinois Composition for blocking hiv binding to dendritic cells and methods of use thereof
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WO2005111627A2 (en) 2004-04-15 2005-11-24 Massachusetts Institute Of Technology Methods and products related to the improved analysis of carbohydrates
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US20060127950A1 (en) 2004-04-15 2006-06-15 Massachusetts Institute Of Technology Methods and products related to the improved analysis of carbohydrates
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CA2674493C (en) 2007-01-05 2017-05-09 Massachusetts Institute Of Technology Compositions of and methods of using sulfatases from flavobacterium heparinum
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US20090286289A1 (en) * 2008-03-10 2009-11-19 Pang Danny Z Production of Hyaluronate Unsaturated Disaccharides and its Application
US9045742B2 (en) * 2009-05-29 2015-06-02 The Arizona Board Of Regents For And On Behalf Of Arizona State University Recombinant Edwardsiella bacterium
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Also Published As

Publication number Publication date
US20040091472A1 (en) 2004-05-13
US20050233419A1 (en) 2005-10-20
EP1532241B1 (de) 2010-09-15
US7105334B2 (en) 2006-09-12
WO2003102160A3 (en) 2004-09-16
US6962699B2 (en) 2005-11-08
US7129335B2 (en) 2006-10-31
ATE481478T1 (de) 2010-10-15
AU2003243396A8 (en) 2003-12-19
EP1532241A2 (de) 2005-05-25
US20050227320A1 (en) 2005-10-13
DE60334220D1 (de) 2010-10-28
AU2003243396A1 (en) 2003-12-19
CA2493509C (en) 2010-03-09
CA2493509A1 (en) 2003-12-11
WO2003102160A2 (en) 2003-12-11
US20070065424A1 (en) 2007-03-22

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