EP1514600B2 - Pipette à piston mécanique - Google Patents

Pipette à piston mécanique Download PDF

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Publication number
EP1514600B2
EP1514600B2 EP03405604A EP03405604A EP1514600B2 EP 1514600 B2 EP1514600 B2 EP 1514600B2 EP 03405604 A EP03405604 A EP 03405604A EP 03405604 A EP03405604 A EP 03405604A EP 1514600 B2 EP1514600 B2 EP 1514600B2
Authority
EP
European Patent Office
Prior art keywords
calibration
pipette
volume
adjustment
piston
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP03405604A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1514600B1 (fr
EP1514600A1 (fr
Inventor
Jean-Pierre Uldry
Sylvain Christen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Socorex Isba SA
Original Assignee
Socorex Isba SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Application filed by Socorex Isba SA filed Critical Socorex Isba SA
Priority to DE60314516T priority Critical patent/DE60314516T3/de
Priority to AT03405604T priority patent/ATE365075T1/de
Priority to ES03405604T priority patent/ES2289252T5/es
Priority to EP03405604A priority patent/EP1514600B2/fr
Priority to US10/916,359 priority patent/US7204163B2/en
Publication of EP1514600A1 publication Critical patent/EP1514600A1/fr
Publication of EP1514600B1 publication Critical patent/EP1514600B1/fr
Publication of EP1514600B2 publication Critical patent/EP1514600B2/fr
Application granted granted Critical
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/148—Specific details about calibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/026—Drum counters

Definitions

  • the present invention relates to a mechanical piston pipette comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged to be provided with a replaceable tip, and a tip ejector device movable along the cylinder. for ejecting a tip from the end of the cylinder, said pipette body accommodating an ejection actuating device and a piston actuating device performing a determined longitudinal translation stroke between a low stop position and a position of high stop for performing the aspiration and dosing of liquids, with means for modifying said stroke.
  • piston pipettes are known in the state of the art. There are mechanical pipettes whose mechanism is entirely operated by the hand of the user. When these pipettes have a volume display means, it can be analog, digital and mechanical, or electronic. There are also many types of piston pipettes operated by an electric motor and whose display devices or adjustment or calibration devices are also performed by electromechanical or electronic means.
  • the present invention relates exclusively to mechanical piston pipettes with fixed or adjustable volume. When the volume is adjustable, the invention relates exclusively to pipettes whose volume display itself is mechanical.
  • Examples of pipettes of the state of the art comprising protruding and prominent "L" -shaped, head or platform top parts include the Eppendorf Reference® pipette, marketed by Eppendorf GmbH, and the Transferpette® pipette, marketed by Brand GmbH & Co. KG.
  • the "Reference” pipette requires a separate universal calibration key, to effect adjustment of the display means
  • the "Transferpette” pipette requires the detachment of several pieces to perform calibration by adjustment of the display means, as described herein. -above.
  • the display means are directed towards the top of the pipette, not allowing a visual control during pipetting.
  • a visual control of the display and calibration device during pipetting that is to say during a work phase where, in general, the palm of the hand is oriented approximately towards the face of the user, and to arrange this device accordingly.
  • the document EP 0649678A1 is particularly concerned with the modification of the correction factor of a piston stroke pipette.
  • This document teaches a calibration in which a modification of the pipette correction factor is made by adjusting the adjusting devices, an operating lever operable from outside the pipette housing.
  • the document US 4,672,857 also relates to a positive displacement pipette in which, during a calibration, the pipette volume and the display are adjusted independently to a zero value using a particular tool.
  • the pipette according to this document has a tip-piston assembly that can be connected to the pipette. During the connection, a calibration of each piston-tip assembly at the zero value is performed, which makes it possible to use piston-tip assemblies whose piston is subjected to changes in length, for example due to autoclaving. .
  • a mechanical piston pipette comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged to be provided with a replaceable tip, and a tip ejector device.
  • said accommodating pipette body an ejecting actuator, and a piston actuating device having a metering knob performing a determined stroke in longitudinal translation between a low abutment position and a high abutment position for effecting the suction of liquids, with means for modifying said stroke
  • means for adjusting the pipetted volume comprising a set screw
  • display means pipetted volume comprising a plurality of counting rings
  • calibration means characterized in that said adjustment means, said display means and said calibration means co-operate in an adjustment and calibration device in which, during a calibration operation, said display means are adjusted so as to make the displayed volume coincide with the actual pipetted volume without modifying the stroke
  • a fixed volume mechanical pipette comprising a pipette body, a piston-cylinder assembly, the cylinder being arranged to be provided with a replaceable tip, and a tip ejector device, movable along the cylinder for ejecting a tip from the end of the cylinder, said pipette body accommodating an ejection actuating device and a piston actuating device performing a determined longitudinal translation stroke between a low stop position and a high stop position for effecting the suction of liquids, with means for modifying said stroke, characterized in that a piece defining the bottom stop of said stroke passes from a fully locked position to a position where it can make a transverse movement, in that, in the locked position, it prevents the longitudinal displacement of a part defining the bottom of the race stop and that in the said unlocked position, it allows the action of calibration means moving the longitudinal position of said travel stop.
  • the adjustable volume pipette body shown by the figure 1 comprises an outer sleeve 14 itself constituted of a longitudinal portion 14a, substantially cylindrical but with a slight ergonomic bulge to fit the palm of the hand, with a substantially elliptical cross section, the upper part of which extends laterally by a comma-shaped stick 14b intended to rest on the flank of the index finger of the user's hand.
  • the outer sleeve 14 accommodates an inner sleeve 13 which is housed in the outer sleeve 14 on the same side as the butt 14b.
  • the inner sleeve 13 has a cylindrical inner wall portion with an arcuate section, which forms with the inner surface of the opposite zone of the outer sleeve 14 a cylindrical housing of circular section.
  • the inner sleeve 13 is sufficiently thick to accommodate the tip ejection device, which comprises an ejector button 10 surmounted by a protective cap 9.
  • the ejector button is extended by an ejector rod 18 on which are threaded a spring 12
  • the inner sleeve 13 also houses a pinion tube 19 comprising a plurality of rotary gears, for example three pinions 19a, 19b, 19c, rotating about an axis parallel to the longitudinal axis of the pinion. the pipette and cooperating with the adjustment and calibration device as will be described later.
  • the top of the sleeve 13 extends laterally by a ring 13a, coaxial with the cylindrical housing, which caps the upper opening of the sleeve 14.
  • a metering knob 3 protrudes from the top of the ring 13a.
  • the metering knob 3 is intended to be pressed into the pipette body by the thumb of the user during the pipetting operations, namely aspiration of liquid into the disposable tip and ejection of this liquid.
  • the metering knob 3 is pushed upwards by the pipetting spring 7, so as to perform reciprocating translational movements along the longitudinal axis of the pipette body.
  • the top of the metering knob 3 is plugged by a cap 1.
  • the cap 1 is easily removed by the user by means of a fingernail. It can be made in different colors to facilitate the visual identification of the pipette.
  • the metering knob 3 is threaded internally. It receives a set screw 4 threaded externally, so that the rotation of the metering knob 3 drives the adjusting screw 4 in longitudinal translation, when it is itself locked in rotation as described below.
  • the adjusting screw 4 is shown in its lower extreme position.
  • the upper part of the adjusting screw 4 is recessed and receives a calibration key 2 which can slide in longitudinal translation in the recess of the adjusting screw 4, but which is locked in rotation. To do this, the recess of the adjusting screw 4 and the calibration key can both be of square section.
  • the calibration key is threaded into a ring 5.
  • the middle portion of the adjusting screw 4 carries a bulge 4a, integrally with the body of the screw 4, on which rests an over-stroke spring 4b.
  • the other end of the overtravel spring 4b rests on an overtravel abutment 4c fitted on and secured to the body of the adjusting screw 4.
  • the adjustment and calibration device is designated as a whole by 8. It comprises a calibration disk 8a.
  • the calibration disk 8a comprises an upper barrel 8b surrounding the body of the adjusting screw 4, which slides there longitudinally, and a lower flange 8c of diameter greater than that of the barrel 8b.
  • the calibration disk 8a rests on the bottom 14c of the outer sleeve 14 and thus limits the stroke of the screw 4, when the lower face of the abutment 4c comes into contact with it.
  • An adjustment tube 8d rests on the horizontal circular plate 8h formed between the flange 8c and the barrel 8b.
  • the adjustment tube 8d and the calibration disk 8a are coupled by a coupling dog system C, consisting of two toothed rings facing each other, respectively formed on the plate 8h of the calibration disc and on the lower end face of the tube. 8d setting. Teeth are low in height and easily disengage.
  • the outer sleeve 14 houses, at the level of the calibration disk 8a, a locking device consisting of a shoe 15 provided with an elastic clip 16. In the locked position, this pad 15 prevents the rotation of the calibration disk 8a. The pad is operable from the outside, by a hole of the sleeve 14, by any object having a fine point.
  • the calibration disk 8a is rotatably secured to the body of the adjusting screw 4 by means of ribs / longitudinal conjugate grooves.
  • the top of the adjustment tube 8d surrounds the bottom of the metering knob 3.
  • the adjustment tube 8d and the metering knob 3 are also secured in rotation by ribs / conjugate grooves.
  • the adjustment tube has a lower portion of outside diameter slightly decreased, forming a horizontal circular projection.
  • the adjustment tube 8d on the other hand carries on this lower part a plurality of counting rings, for example three rings 8e, 8f, 8g bearing on their periphery numbers from 0 to 9, providing a digital indication of the volume of liquid pipetted. .
  • the outer sleeve 14 has on its side opposite to the butt 14b and at the height of the counting rings a transparent window 6 for reading the volume indication.
  • the projection of the adjustment tube 8d facing the counting rings has several teeth driving the pinion 19a.
  • the counting rings have a continuous toothing on the upper end face but a single indentation on the opposite end face, so that the rotation of the adjustment tube 8d drives the first pinion, which in turn drives the first disk 8e which, by example after one complete revolution, drives the second pinion 19b rotating a digit the second count ring, and so on. This mechanism is known per se.
  • the locking shoe 15 is in the locked position, preventing the rotation of the calibration disk 8a.
  • the calibration disc blocks the rotation of the adjusting screw 4, which itself is rotatably connected to the calibration key 2.
  • the user rotates the metering knob 3, which, by its internal thread, drives in translation the adjusting screw 4.
  • This displacement in longitudinal translation operates the adjustment of the stroke extending between the bottom of the travel stop 4c and the top of the calibration disc 8a.
  • This stroke itself determines the volume of liquid, since the adjusting screw 4 is connected to the piston of the pipette by its lower end 4d shaped spherical head constituting a detachable connection.
  • the rotation of the metering knob 3 rotates the adjustment tube 8d and, via the gear tube 19, the meter disks 8e, 8f, 8g, as explained above.
  • the jaw system C is uncoupled, the tube 8d rotates on the calibration disc 8a, the teeth of the toothed segments ripening one above the other.
  • a calibration operation is performed as follows:
  • the volume dispensed by the pipette is measured, for example by weighing it.
  • the measured volume may be 545 ⁇ l while the meter displays 210 ⁇ l.
  • the calibration operation consists in bringing the value of the display to 545 ⁇ l corresponding to the effective volume, without modifying the stroke.
  • the locking shoe 15 is unlocked, thus releasing the rotation of the calibration disk 8a.
  • the cap 1 is removed to access the calibration key 2.
  • the latter can be actuated either by a coin or a screwdriver, or, after having partially slid outwards, can be operated by hand.
  • the rotation of the calibration key rotates the adjusting screw 4, which itself rotates the calibration disk.
  • the user makes sure not to touch the metering knob 3 so as not to change the relative position knob 3 / screw 4.
  • the calibration disk drives the adjusting tube 8d in rotation, by through the jaw system C.
  • the rotation of the adjustment tube causes the rotation of the pinion tube sprockets 19 and therefore the rotation of the counting rings.
  • the key is rotated until the measured value, in this example 545 ⁇ l, is displayed. Then, the pad 15 is pushed into the locking position to lock in angular position the calibration disc 8a and the adjusting screw 4. The calibration key 2 is pushed into its housing and the cap is replaced. Since the value of the counter device is modeled on the stroke, the subsequent volume adjustments of the pipette are made simply by rotation of the metering knob 3, which this time changes the stroke by translational movement of the adjusting screw 4 without rotating movement. of it.
  • the fixed volume pipette body shown by the figure 2 comprises an outer sleeve 14 itself constituted of a longitudinal portion 14a, substantially cylindrical but with a slight ergonomic bulge to fit the palm of the hand, with a substantially elliptical cross section, the upper part of which extends laterally by a comma-shaped stick 14b intended to rest on the flank of the index finger of the user's hand.
  • the outer sleeve 14 accommodates an inner sleeve 13 which is housed in the outer sleeve 14 on the same side as the butt 14b.
  • the inner sleeve 13 has a cylindrical inner wall portion with an arcuate section, which forms with the inner surface of the opposite zone of the outer sleeve 14 a cylindrical housing of circular section.
  • the inner sleeve 13 is sufficiently thick to accommodate the tip ejection device, which comprises an ejector button 10 surmounted by a protective cap 9.
  • the ejector button is extended by an ejector rod 18 on which are threaded a spring 12 and a socket 11.
  • the location of the pinion tube 19 described above remains empty.
  • the top of the sleeve 13 extends laterally by a ring 13a, coaxial with the cylindrical housing, which caps the upper opening of the sleeve 14.
  • a metering knob 3 protrudes from the top of the ring 13a.
  • the metering knob 3 is intended to be pressed into the pipette body by the thumb of the user during the pipetting operations, namely aspiration of liquid into the disposable tip and ejection of this liquid.
  • the metering knob 3 is pushed upwards by the pipetting spring 7, so as to perform reciprocating translational movements along the longitudinal axis of the pipette body.
  • the top of the metering knob 3 is clogged by a cap 1.
  • the cap 1 is easily removed by the user by means of a fingernail. It can be made in different colors to facilitate the visual identification of the pipette.
  • the metering knob 3 is threaded internally. It receives a setting screw 4 externally threaded.
  • a setting screw 4 externally threaded.
  • the adjusting screw 4 is shown in its lower extreme position.
  • the upper part of the adjusting screw 4 is recessed and receives a calibration key 2 which can slide in longitudinal translation in the recess of the adjusting screw 4, but which is locked in rotation. To do this, the recess of the adjusting screw 4 and the calibration key can both be of square section.
  • the calibration key is threaded into a ring 5.
  • the middle portion of the adjusting screw 4 carries a bulge 4a, integrally with the body of the screw 4, on which rests an over-stroke spring 4b.
  • the other end of the overtravel spring 4b rests on an overtravel abutment 4c fitted on and secured to the body of the adjusting screw 4.
  • the calibration device is designated as a whole by 8 '. It includes a calibration disk 8a.
  • the calibration disk 8a comprises an upper barrel 8b surrounding the body of the adjusting screw 4, which slides there longitudinally, and a lower flange 8c of diameter greater than that of the barrel 8b.
  • the calibration disk 8a rests on the bottom 14c of the outer sleeve 14 and thus limits the stroke of the screw 4, when the lower face of the abutment 4c comes into contact with it.
  • the calibration disk 8a may be identical to that shown on the figure 1 , and carry a circular ring gear formed on the plate 8h of the calibration disk; but, in the case of a fixed volume pipette, this crown has no function.
  • the outer sleeve 14 houses, at the level of the calibration disk 8a, a locking device consisting of a shoe 15 provided with an elastic clip 16. In the locked position, this pad 15 prevents the rotation of the calibration disk 8a.
  • the pad is operable from the outside, by a hole of the sleeve 14, by any object having a fine point.
  • the calibration disk 8a is rotatably secured to the body of the adjusting screw 4 by means of ribs / longitudinal conjugate grooves.
  • the tube 8d has like the tube 8d described above a lower portion of outside diameter slightly decreased, forming a horizontal circular projection. But the tube 8d 'does not wear counting rings.
  • the outer sleeve 14 has on its side opposite to the butt 14b and at the height of the lower part of the tube 8d an opaque plate 6 'replacing the window 6.
  • the volume dispensed by the pipette is measured, for example by weighing it.
  • the measured volume may be 545 ⁇ l while the nominal value is 500 ⁇ l.
  • the calibration operation consists of bringing the value of the actual volume to the nominal value.
  • the locking shoe 15 is unlocked, thus releasing the rotation of the calibration disk 8a.
  • the cap 1 is removed to access the calibration screw 2.
  • the latter can be actuated either by a screwdriver or a coin, or, after having partially slid outwards, can be actuated by hand.
  • the rotation of the calibration key rotates the adjusting screw 4, which itself rotates the calibration disk.
  • the calibration disc slides under the tube 8d '.
  • the adjusting screw moves in longitudinal translation, modifying the stroke, and thus the pipetted volume. After measuring this volume, the operation may be repeated until the measured value coincides with the nominal value or with another value desired by the user.
  • the pad 15 is pushed into the locking position to lock in angular position the calibration disc 8a and the adjusting screw 4.
  • the calibration key 2 is pushed into its housing and the cap is replaced.
  • variable volume pipette body and the fixed volume pipette body use, in the same volume range, a large majority of common parts. These are identified by identical reference numbers in the figures 1 and 2 .
  • the same piece, the calibration ring 8a provides several functions, namely stop limit, adjustment and / or calibration in both types of pipette.
  • a single essential piece, namely the tube 8d, respectively 8d ', has different structures and functions: the tube 8d serves both as a guide to the metering knob 3, as a housing to the spring 7 and provides a setting function as exposed above.
  • the tube 8d ' which differs essentially from the tube 8d by the presence of outer ribs blocking it in rotation serves only as a guide and as a housing. It will be noted that the tubes 8d and 8d 'also have identical dimensions.
  • the adjustable volume pipette body further has a set of counting rings and drive gears described above, which are not not present in the body of the fixed volume pipette.
  • variable volume pipette the dimensions of the body of the variable volume pipette are not different from the dimensions of the fixed volume pipette body, the set of counting rings and drive gears being housed respectively in the overall size of the adjustment tube 8d and coaxially with the ejection device, inside the inner sleeve 13.
  • the entire adjustment and calibration device is housed in the longitudinal pipette body, without increasing its volume, and the display, which is not hidden by the thumb, remains visible to the operator holding his pipette in a natural way.
  • the arrangement of the counting discs and drive gears allows in particular, without increasing the volume of the body of the pipette, to arrange the ejection button 10 above the butt of the outer sleeve, which is ergonomically favorable, the user's thumb more easily pressing the eject button when it comes over the edge of the hand than when it deviates from it, which would be the case if the button ejection had been placed on the opposite side.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Steroid Compounds (AREA)
EP03405604A 2003-08-19 2003-08-19 Pipette à piston mécanique Expired - Lifetime EP1514600B2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE60314516T DE60314516T3 (de) 2003-08-19 2003-08-19 Mechanische Kolbenpipette
AT03405604T ATE365075T1 (de) 2003-08-19 2003-08-19 Mechanische kolbenpipette
ES03405604T ES2289252T5 (es) 2003-08-19 2003-08-19 Pipeta de pistón mecánica
EP03405604A EP1514600B2 (fr) 2003-08-19 2003-08-19 Pipette à piston mécanique
US10/916,359 US7204163B2 (en) 2003-08-19 2004-08-11 Mechanical piston pipette

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03405604A EP1514600B2 (fr) 2003-08-19 2003-08-19 Pipette à piston mécanique

Publications (3)

Publication Number Publication Date
EP1514600A1 EP1514600A1 (fr) 2005-03-16
EP1514600B1 EP1514600B1 (fr) 2007-06-20
EP1514600B2 true EP1514600B2 (fr) 2012-02-22

Family

ID=34130400

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03405604A Expired - Lifetime EP1514600B2 (fr) 2003-08-19 2003-08-19 Pipette à piston mécanique

Country Status (5)

Country Link
US (1) US7204163B2 (es)
EP (1) EP1514600B2 (es)
AT (1) ATE365075T1 (es)
DE (1) DE60314516T3 (es)
ES (1) ES2289252T5 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE480330T1 (de) * 2001-10-16 2010-09-15 Matrix Technologies Corp Hand-pipettiervorrichtung
DE102005033378B4 (de) * 2005-07-16 2012-05-31 Eppendorf Ag Kolbenhubpipette
PL211690B1 (pl) * 2007-11-23 2012-06-29 Pz Htl Społka Akcyjna Pipeta z nastawianą objętością pobieranej cieczy
WO2009114350A1 (en) * 2008-03-07 2009-09-17 Cook Vascular Incorporated Pipette aspiration device
DE102008048252A1 (de) * 2008-09-12 2010-04-15 Eppendorf Ag Pipettiervorrichtung
PL220934B1 (pl) * 2009-08-28 2016-01-29 PZ HTL Spółka Akcyjna Pipeta mechaniczna z nastawianą wartością objętości pobieranej cieczy
FI20106184A0 (fi) 2010-11-11 2010-11-11 Thermo Fisher Scientific Oy Kahvapipetti
US9295986B2 (en) * 2012-05-02 2016-03-29 Eppendorf Ag Pipette with releasable locking of rotational position of actuating element
US10016755B2 (en) 2015-01-08 2018-07-10 Integra Biosciences Ag Manual pipette with selectable plunger force
CN107530702B (zh) * 2015-04-16 2019-06-11 因特格拉生物科学股份公司 用于手动移液器的容量调节
FR3040896B1 (fr) * 2015-09-15 2017-10-13 Gilson Sas Pipette de prelevement comprenant un organe de commande a double fonction d'ejection de cone et de deverrouillage du systeme de reglage de volume
FR3049877B1 (fr) * 2016-04-12 2018-04-13 Gilson Sas Pipette pour le prelevement d'une plage etendue de volumes de liquide
DE102016121814A1 (de) * 2016-11-14 2018-05-17 Ika-Werke Gmbh & Co. Kg Handdosiervorrichtung
EP3684513A1 (en) * 2017-09-19 2020-07-29 Mettler-Toledo Rainin, LLC Pipette calibration and volume offset mechanism
FR3080050B1 (fr) * 2018-04-17 2020-03-27 Gilson Sas Dispositif de verrouillage d'une vis de reglage de volume pour un systeme de pipetage
DE102020002780B3 (de) 2020-05-11 2021-10-14 Brand Gmbh + Co Kg Pipette und Verfahren zum Betreiben einer Pipette
CN113634297A (zh) 2020-05-11 2021-11-12 普兰德有限两合公司 移液器和用于运行移液器的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4165646A (en) 1978-07-26 1979-08-28 Shapiro Justin J Adjustable micro-dispensing liquid pipet
FI840579A0 (fi) * 1984-02-14 1984-02-14 Labsystems Oy Pipett med reglerbar volym
FR2696110B1 (fr) * 1992-09-28 1994-12-09 Gilson Medical Electronics Sa Perfectionnement à une piquette de prélèvement et de distribution de volumes réglables de liquides.
DE4335863C1 (de) * 1993-10-21 1995-02-02 Eppendorf Geraetebau Netheler Kolbenhubpipette
US5650124A (en) * 1995-07-24 1997-07-22 Gilson; Warren E. Adjustable pipette
US5792424A (en) * 1996-03-05 1998-08-11 Rainin Instrument Co., Inc. Manual pipette with delayed-action home position latch
US5998218A (en) * 1996-09-09 1999-12-07 Sherwood Services Ag Calibration system for an electronically monitored mechanical pipette
US6295880B1 (en) * 1999-12-08 2001-10-02 Warren E. Gilson Adjustable pipette
FR2807343B1 (fr) * 2000-04-07 2002-12-06 Gilson Sa Pipette de prelevement munie de moyens de reglage du volume a prelever
FI20002241A0 (fi) * 2000-10-11 2000-10-11 Labsystems Oy Säädettävä pipetti

Also Published As

Publication number Publication date
EP1514600B1 (fr) 2007-06-20
DE60314516T2 (de) 2008-02-28
DE60314516T3 (de) 2012-07-19
US20050039550A1 (en) 2005-02-24
ES2289252T3 (es) 2008-02-01
ES2289252T5 (es) 2012-06-12
EP1514600A1 (fr) 2005-03-16
DE60314516D1 (de) 2007-08-02
ATE365075T1 (de) 2007-07-15
US7204163B2 (en) 2007-04-17

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