EP1506299A2 - Procede de fabrication d'une plante transgenique presentant un transport de matiere modifie - Google Patents

Procede de fabrication d'une plante transgenique presentant un transport de matiere modifie

Info

Publication number
EP1506299A2
EP1506299A2 EP03724887A EP03724887A EP1506299A2 EP 1506299 A2 EP1506299 A2 EP 1506299A2 EP 03724887 A EP03724887 A EP 03724887A EP 03724887 A EP03724887 A EP 03724887A EP 1506299 A2 EP1506299 A2 EP 1506299A2
Authority
EP
European Patent Office
Prior art keywords
nucleotide sequence
seq
sequence
dna
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03724887A
Other languages
German (de)
English (en)
Inventor
Wolf-Bernd Frommer
Mechthild Tegeder
Axel Hirner
Wolfgang Koch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP1506299A2 publication Critical patent/EP1506299A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Definitions

  • the invention relates to a method for producing a transgenic plant in which the ability to store undesired nitrogen compounds, in particular amino acids, in the harvesting and / or reproductive organs is reduced compared to the wild type.
  • the invention further relates to DNA sequences and RNA sequences corresponding to them, which contain the coding region for an amino acid transporter or parts thereof, their use and vectors, mobile genetic elements, bacteria, host cells, plant cells, plants, seeds and other plant propagation material, which contain the DNA and / or RNA sequences.
  • Plants absorb nitrogen, which is required to build up cell substance, mainly in the form of nitrate, but also as ammonium and, to a lesser extent, as amino acids, for example via the roots.
  • the nitrate is reduced to amino nitrogen and incorporated into organic compounds. This mainly happens in the leaves of the plant.
  • Nitrogen-containing organic compounds, for example amino acids are transported via a vascular system, the phloe, from places of formation such as the leaves to consumption tissues and organs, for example reproductive organs or storage organs (roots, tubers, beets, etc.).
  • the transport processes are particularly important for the shifting of nitrogen compounds in the course of the aging (senescence) of leaves, for example towards the end of the growing season. This removes nitrogen compounds from the dying leaves into other organs, for example Storage organs, relocated to minimize the loss of cell material.
  • AAPs not only mediate the transport of a broad spectrum of amino acids with comparatively low selectivity, but also of other organic nitrogen compounds, for example amides such as glutamine and asparagine as well as citrulline, gamma-aminobutyric acid or auxin. They therefore presumably play a key role in the distribution of organic nitrogen compounds within the plant.
  • Amino acid transporter genes from Arabidopsis thaliana are known for example from EP 0652955.
  • the storage of organic nitrogen compounds such as proteins and amino acids in crop organs of plants is often undesirable.
  • the object of the present invention is therefore to create a possibility of producing plants in which the incorporation of organic nitrogen compounds in their harvesting organs is reduced compared to the wild type.
  • a method which comprises the steps of introducing at least one DNA sequence and / or an RNA sequence corresponding to the DNA sequence and / or a mixed sequence composed of DNA and RNA nucleotides with the coding sequence Region for an amino acid transporter or parts thereof in a plant cell, the DNA and / or RNA sequence and / or mixed sequence in sense or antisense orientation is used and the expression of an endogenous amino acid transporter gene is prevented or reduced, and regenerating a plant from this plant cell, wherein the DNA and / or RNA sequence is a sequence from Beta vulgaris.
  • Plants which have been produced by the process according to the invention have a significantly reduced content of organic nitrogen compounds, in particular amino acids, in their harvesting organs.
  • a plant cell into which the DNA sequence and / or the RNA sequence corresponding to the DNA sequence and / or the RNA / DNA mixed sequence corresponding to the DNA sequence has been introduced in the antisense orientation, the translation of the mRNA of the endogenous amino acid transporter gene hindered by the attachment of an antisense RNA to the RNA.
  • the antisense RNA is formed by transcription of the DNA sequence. In the case of the RNA sequence corresponding to the DNA sequence, this can itself represent the antisense RNA.
  • Post-transcriptional muting post-transcriptional gene silencing
  • PGS post-transcriptional gene silencing
  • RNA interference in that the DNA sequence or an RNA sequence corresponding to the DNA sequence is sense-oriented into the plant cell is introduced, the RNA being used as double-stranded RNA in the case of RNA interference.
  • mixed sequences composed of RNA and DNA sequences chimeric oligonucleotides, as described, for example, by Rice et al. (2000), Plant Physiology 123, 427-437, can be used to mute the endogenous amino transporter gene.
  • the plants produced by the process according to the invention are largely prevented in this way from forming amino acid transporters.
  • tissue-specific promoters By coupling to suitable tissue-specific promoters, it is also possible to specifically target the formation of amino acid transporters in certain tissues or organs of the plant, such as in the leaves, to reduce or suppress.
  • parts of a coding region used here denotes nucleotide sequences with at least 20 nucleotides which, in the antisense orientation, enable repression of an amino acid transporter gene.
  • an RNA sequence corresponding to the DNA sequence denotes an RNA sequence which has the same sequence of the purine and pyrimidine bases as a DNA sequence, but has the base uracil instead of the base thymine in the DNA sequence ,
  • a mixed sequence composed of DNA and RNA nucleotides corresponding to the DNA sequence denotes a nucleotide sequence which has the same sequence of the purine and pyrimidine bases as a DNA sequence, but which has both DNA nucleotides and RNA - Contains nucleotides, and wherein the RNA nucleotides have the base uracil instead of the base thymine in the DNA sequence.
  • Chimeric oligonucleotides represent such mixed sequences, for example.
  • Antisense orientation of a DNA sequence here means that transcription of the DNA sequence results in an mRNA whose nucleotide sequence is complementary to the natural one
  • RNA sequence is complementary to an endogenous mRNA and its translation is hindered or prevented by attachment.
  • a DNA sequence used in the method according to the invention preferably comprises the nucleotide sequence of SEQ ID NO: 5 or a nucleotide sequence complementary to this nucleotide sequence. sequence, or hybridizes with the nucleotide sequence of SEQ ID NO: 5 or a nucleotide sequence that is complementary to the nucleotide sequence of SEQ ID NO: 5.
  • the DNA sequence can also, alternatively or additionally, the nucleotide sequence (s) of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO : 7 or SEQ ID NO: 8 or a nucleotide sequence complementary to this nucleotide sequence, or with the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO : 6, SEQ ID NO: 7 or SEQ ID NO: 8 or a nucleotide sequence which corresponds to the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8 is complementary, hybridize.
  • hybridizing means hybridizing under usual conditions, as described in Sa brook et al. (Molecular Cloning. A laboratory manual, Cold Spring Harbor Laboratory Press, 2nd ed., 1989), preferably under stringent conditions.
  • Stringent hybridization conditions are, for example: hybridization in 4 x SSC at 65 ° C and subsequent multiple washing in 0.1 x SSC at 65 ° C for a total of about 1 hour.
  • Hybridization conditions that are not very stringent are, for example: hybridization in 4 x SSC at 37 ° C. and then repeated washing in 1 x SSC at room temperature.
  • stringent hybridization conditions used here can also mean: hybridization at 68 ° C. in 0.25 M sodium phosphate, pH 7.2, 7% SDS, 1 mM EDTA and 1% BSA for 16 hours and subsequent washing twice with 2 ⁇ SSC and 0.1% SDS at 68 ° C.
  • the invention also relates to DNA sequences which contain the coding region for an amino acid transporter or parts thereof, RNA sequences corresponding to the DNA sequences and mixed sequences composed of RNA and DNA nucleotides corresponding to the DNA sequences.
  • the DNA sequence comprises the nucleotide sequence of SEQ ID NO: 1 or a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 1, or hybridizes with the nucleotide sequence of SEQ ID NO: 1 or a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 1.
  • the DNA sequence comprises the nucleotide sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8 or one complementary to the nucleotide sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8 Nucleotide sequence, or hybridizes with the nucleotide sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8 or a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8 ,
  • the invention also relates to vectors or mobile genetic elements which comprise at least one DNA sequence and / or at least one RNA sequence corresponding to the DNA sequence and / or at least one mixed sequence composed of DNA and RNA nucleotides according to the DNA sequence present invention included.
  • Vectors or mobile genetic elements which are suitable for introducing nucleotide sequences into host cells, for example viruses, bacteriophages, cosmids, plasmids, artificial yeast chromosomes, T-DNA, transposons, insertion sequences etc., are well known to those skilled in the field of molecular cloning techniques.
  • the DNA sequence (s) according to the invention and / or the RNA sequence (s) corresponding to the DNA sequence (s) and / or the DNA sequence (s) is / are preferred ) contain corresponding mixed sequence (s) of DNA and RNA nucleotides in antisense orientation in the vector or the mobile genetic element.
  • the RNA sequence (s) are / are contained in an RNA double strand in the vector or the mobile genetic element. This enables the endogenous amino acid transporter gene to be muted by RNA interference.
  • the invention further relates to eukaryotic or prokaryotic host cells which contain at least one DNA and / or at least one RNA sequence corresponding to this and / or at least one mixed sequence composed of DNA and RNA nucleotides corresponding to the DNA sequence according to the present invention , wherein the DNA, RNA and / or DNA-RNA mixed sequence (s) is / are preferably contained in the host cell in an antisense orientation.
  • RNA sequence (s) are / are contained in an RNA double strand in the eukaryotic or prokaryotic host cell.
  • the invention also relates to plants and parts or seeds of plants which are composed of at least one DNA sequence and / or at least one RNA sequence corresponding to the DNA sequence and / or at least one corresponding to the DNA sequence of DNA and RNA nucleotides Mixing sequence are transformed according to the present invention.
  • the plants can, for example, be plants of the Beta genus, preferably of the Beta vulgaris species.
  • numerous other plants are also possible, for example potatoes, tomatoes, sugar cane, tobacco, rapeseed, ricinus, etc.
  • plants are available in which the expression of an amino acid transporter gene, for example by means of co-suppression through interaction with a homologous ectopic (out of position) gene sequence is suppressed.
  • the transgenic plants, their parts or their seeds are preferably transformed with the DNA and / or RNA and / or DNA-RNA mixing sequence in the antisense orientation.
  • the transcription of the DNA sequence in the antisense orientation forms an mRNA which at least partially hybridizes with the naturally formed mRNA for the amino acid transporter in such a way that translation into the corresponding protein cannot take place.
  • the introduction of an RNA sequence in an antisense orientation leads to an attachment of the RNA sequence to the naturally formed mRNA, so that the translation of the mRNA is also impeded in this case.
  • transgenic plants their parts or their seeds, which have been transformed with an RNA sequence in an RNA double strand.
  • the present invention further relates to the use of DNA, RNA and / or DNA-RNA mixed sequences according to the invention for producing a transgenic plant cell or plant with an increased expression of the coding region of the amino acid transporter compared to the wild type.
  • the sequences according to the invention are used in a sense orientation. This makes it possible, for example, to obtain plants which, compared to wild-type plants, increasingly incorporate nitrogen compounds. In conjunction with suitable promoters, it is also possible to influence the increased incorporation of nitrogen compounds in a tissue-specific manner. In this way, plants, for example soybean plants, can be produced whose harvestable parts have an increased content of organic nitrogen compounds compared to wild type plants.
  • the invention further relates to the use of the DNA sequence and / or a corresponding RNA sequence and / or a mixed sequence corresponding to the DNA sequence and composed of DNA and RNA nucleotides according to the present invention in an antisense orientation for the production of a transgenic plant cell or Plant with a reduced expression of the coding region of the amino acid transporter compared to the wild type.
  • transgenic plants have a reduced ability compared to wild type plants to store organic nitrogen compounds in storage organs.
  • the organic nitrogen compounds predominantly formed in the leaves are not or only to a lesser extent transported into the storage organs in the senescence phase, since the required amino acid transporter molecules are not available in sufficient quantities.
  • sequence listing (according to WIPO standard St. 25) contains:
  • SEQ ID NO: 1 A nucleotide sequence of the coding region of the AAPI gene from Beta vulgaris.
  • SEQ ID NO: 2 A nucleotide sequence of the coding region of the AAP6 gene from Beta vulgaris.
  • SEQ ID NO: 3 A nucleotide sequence of the coding region of the AAP2 gene from Beta vulgaris.
  • SEQ ID NO: 4 A nucleotide sequence of the coding region of the AAP3 gene from Beta vulgaris.
  • SEQ ID NO: 5 A nucleotide sequence of the coding region of the GAPI gene from Beta vulgaris.
  • SEQ ID NO: 6 A nucleotide sequence of the coding region of another AAP gene from Beta vulgaris.
  • SEQ ID NO: 7 A nucleotide sequence (BvProT-like) of the coding region of a further AAP gene from Beta vulgaris.
  • SEQ ID NO: 8 A nucleotide sequence (BvSV2-like) of the coding region of another AAP gene from Beta vulgaris.
  • BvGAPl The amino acid transporter encoded by SEQ ID NO: 5 (BvGAPl) has proven to be a transporter with a wide range of amino acids. "GAP” therefore also stands for "general amino acid permease”. BvGAPl transports gamma-aminobutyric acid (GABA) particularly effectively. Acidic amino acids such as aspartate are also transported particularly well, citrulline, lysine and histidine are also transported,
  • amino acid transporters encoded by SEQ ID NO: 7 (BvProT-like) and SEQ ID NO: 8 (BvSV2-like) specifically transport proline, citrulline and gamma-aminobutyric acid (GABA).
  • FIG. 1 shows: Expression of BvAAP genes (BvAAPl, BvAAP2 and BvAAP6) in different senescence stages of leaves and in five and six month old storage roots of Beta vulgaris, determined by Northern hybridization. 18s rRNA served as a control. It means: leaf RNA: RNA from leaves beet RNA: RNA from storage roots
  • RT-PCR reverse transcription followed by polymerase chain reaction
  • three nucleotide sequences were isolated and sequenced from senescent leaves and storage root material of the sugar beet (beta vulgaris), which encode the coding region of amino acid transporters (BvAAPl and BvAAP2, BvAAP6), or parts thereof , include.
  • CDNA obtained from the nucleotide sequences was cloned into the yeast expression vector pDRl96.
  • the vector pBin19 and derivatives thereof are suitable as vectors for the transformation of sugar beets. Again, cloning in antisense Orientation possible, for example, under the control of the CaMV-35S Pro otor.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de fabrication d'une plante transgénique dont la capacité de stockage de composés azotés indésirables, notamment d'acides aminés, dans les organes de stockage, est réduite par rapport au type sauvage. Ledit procédé consiste à introduire dans les cellules végétales, au moins une séquence d'ADN et/ou au moins une séquence d'ARN correspondant à la séquence d'ADN, et/ou une séquence mixte composée de nucléotides d'ADN et d'ARN correspondant à la séquence d'ADN, présentant la région codante pour un transporteur d'acides aminés, ou des parties de cette région. Selon l'invention, la séquence d'ADN et/ou d'ARN et/ou la séquence mixte est employée en orientation sens ou antisens, et l'expression d'un gène transporteur d'acides aminés endogènes est inhibée ou réduite. Ledit procédé consiste ensuite à régénérer une plante à partir de ces cellules végétales, la séquence d'ADN et/ou d'ARN étant une séquence provenant de Beta vulgaris.
EP03724887A 2002-05-13 2003-05-12 Procede de fabrication d'une plante transgenique presentant un transport de matiere modifie Withdrawn EP1506299A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10221224 2002-05-13
DE10221224A DE10221224A1 (de) 2002-05-13 2002-05-13 Verfahren zur Herstellung einer transgenen Pflanze mit verändertem Stofftransport
PCT/DE2003/001512 WO2003095654A2 (fr) 2002-05-13 2003-05-12 Procede de fabrication d'une plante transgenique presentant un transport de matiere modifie

Publications (1)

Publication Number Publication Date
EP1506299A2 true EP1506299A2 (fr) 2005-02-16

Family

ID=29413771

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03724887A Withdrawn EP1506299A2 (fr) 2002-05-13 2003-05-12 Procede de fabrication d'une plante transgenique presentant un transport de matiere modifie

Country Status (5)

Country Link
US (1) US20050235376A1 (fr)
EP (1) EP1506299A2 (fr)
AU (1) AU2003229288A1 (fr)
DE (2) DE10221224A1 (fr)
WO (1) WO2003095654A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007089610A1 (fr) 2006-01-26 2007-08-09 Ceres, Inc. Modulation des taux d'huile dans des plantes
WO2006062971A2 (fr) 2004-12-08 2006-06-15 Ceres Inc. Modulation des teneurs en carbone dans les plantes
BRPI0519657A2 (pt) 2004-12-16 2009-03-03 Ceres Inc modulaÇço dos nÍveis de nitrogÊnio em plantas
US7335760B2 (en) 2004-12-22 2008-02-26 Ceres, Inc. Nucleic acid sequences encoding zinc finger proteins
CN112410309B (zh) * 2020-11-27 2022-02-08 河南农业大学 GmAAP蛋白和GmAAP基因在大豆育种中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4222315A1 (de) * 1992-07-05 1994-01-13 Inst Genbiologische Forschung DNA-Sequenzen für Aminosäuretransporter, Plasmide, Bakterien, Hefen und Pflanzen enthaltend einen Transporter
US6165792A (en) * 1998-08-20 2000-12-26 E. I. Du Pont De Nemours And Company Amino acid transporters
AU6795000A (en) * 1999-08-18 2001-03-13 Curagen Corporation Defense-related signaling genes and methods of use
US6936467B2 (en) * 2000-03-27 2005-08-30 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
AU2001286811B2 (en) * 2000-08-24 2007-03-01 Syngenta Participations Ag Stress-regulated genes of plants, transgenic plants containing same, and methods of use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO03095654A2 *

Also Published As

Publication number Publication date
US20050235376A1 (en) 2005-10-20
DE10221224A1 (de) 2003-12-04
AU2003229288A8 (en) 2003-11-11
AU2003229288A1 (en) 2003-11-11
DE10393079D2 (de) 2005-05-12
WO2003095654A3 (fr) 2004-07-29
WO2003095654A2 (fr) 2003-11-20

Similar Documents

Publication Publication Date Title
EP0765393B1 (fr) Molecules d'adn codant pour un translocateur plastidial de 2-oxoglutarate/malate
DE102004047056B4 (de) Kartoffeln mit erhöhter Ausbeute an Stärke pro Pflanzenkörper und Verfahren zur Herstellung derselben
DE4337597C2 (de) DNA-Sequenzen für Ammoniumtransporter, Plasmide, Bakterien, Hefen, Pflanzenzellen und Pflanzen enthaltend den Transporter
DE4444460A1 (de) Verfahren zur Steigerung des Ertrags sowie zur Veränderung des Blühverhaltens bei Pflanzen
EP0651812B1 (fr) Promoteur recombine modulaire
WO2003095654A2 (fr) Procede de fabrication d'une plante transgenique presentant un transport de matiere modifie
DE4222315A1 (de) DNA-Sequenzen für Aminosäuretransporter, Plasmide, Bakterien, Hefen und Pflanzen enthaltend einen Transporter
EP2478007B1 (fr) Inhibition de la montaison et de la floraison d'une plante de betterave à sucre
DE69633568T2 (de) Expressionskontrollesequenz zur allgemeinen und effektiven genexpression in pflanzen
WO2000031281A2 (fr) Sequence d'adn codant un translocateur de glutamate/malate, plasmides, bacteries, levures et vegetaux contenant ledit transporteur
DE60310690T2 (de) An der synthese von brassinosteroid beteiligtes gen
DE4439748A1 (de) Verfahren zur Veränderung des Blühverhaltens bei Pflanzen
WO1998021336A2 (fr) Procede de controle genetique de la maturation de semences dans des plantes
EP1071757B1 (fr) Association de genes pour la regulation de l'induction de la floraison chez les plantes cultivees et les plantes d'ornement
EP0973908B1 (fr) Plantes avec formation controlee de pousses laterales et/ou formation controlee de zones d'abscission
DE10138091A1 (de) Verfahren zur Beeinflussung der Mineralstoffaufnahme in transgenen Pflanzen
EP1276882A2 (fr) Procede de modification genetique d'une plante
DE69932431T3 (de) Verfahren zum transformieren von pflanzen, die resultierende pflanze und deren gene
WO2002014363A2 (fr) Influence de la repartition de metaux dans des plantes transgeniques
EP2333077B1 (fr) Promoteur induit au stockage
WO1997025346A1 (fr) Sequence d'adn codant pour un translocateur de phosphoenolpyruvate-phosphate, plasmides, bacteries, levures et plantes contenant ce transporteur
EP1190081B1 (fr) Plantes a expression genique modifiee
DE19836774A1 (de) Protein und DNA-Sequenz der Pinosylvin-3-0-methyltransferase (PMT)
DE19732926A1 (de) DNA-Sequenzen kodierend einen Glukose-6-phosphat-Phosphat-Translokator, Plasmide, Bakterien, Hefen und Pflanzen enthaltend diesen Transporter
WO2002050109A2 (fr) Acides nucleiques codant pour des invertases vacuolaires, cellules vegetales et plantes contenant ces acides nucleiques, ainsi que leur utilisation

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK

17P Request for examination filed

Effective date: 20050131

RIN1 Information on inventor provided before grant (corrected)

Inventor name: KOCH, WOLFGANG

Inventor name: HIRNER, AXEL

Inventor name: TEGEDER, MECHTHILD

Inventor name: FROMMER, WOLF-BERND

RIN1 Information on inventor provided before grant (corrected)

Inventor name: KOCH, WOLFGANG

Inventor name: HIRNER, AXEL

Inventor name: TEGEDER, MECHTHILD

Inventor name: FROMMER, WOLF-BERND

DAX Request for extension of the european patent (deleted)
17Q First examination report despatched

Effective date: 20070625

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20071106