EP1504262A1 - Procede pour analyser la fonction plaquettaire du sang - Google Patents

Procede pour analyser la fonction plaquettaire du sang

Info

Publication number
EP1504262A1
EP1504262A1 EP03749845A EP03749845A EP1504262A1 EP 1504262 A1 EP1504262 A1 EP 1504262A1 EP 03749845 A EP03749845 A EP 03749845A EP 03749845 A EP03749845 A EP 03749845A EP 1504262 A1 EP1504262 A1 EP 1504262A1
Authority
EP
European Patent Office
Prior art keywords
aperture
blood
determined
piston
straight line
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03749845A
Other languages
German (de)
English (en)
Inventor
Michael Dr. Kratzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP1504262A1 publication Critical patent/EP1504262A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4905—Determining clotting time of blood

Definitions

  • the present invention relates to a method and a device for examining the platelet function of the blood.
  • EP 0223044 B1 shows a device in which the blood is sucked from a blood storage space through an aperture with the aid of a piston which can be moved in a cylinder, and the pressure in the space between the piston and the sucked-in blood is measured, the Piston is moved by a drive so that a target pressure value is kept in the room. The movement of the piston serves as a measure of the amount of blood flow.
  • the object of the present invention is to provide a method and a device which enable an exact determination of the platelet function of the blood.
  • the main advantage of the present invention is that an exact determination of the platelet delay time is possible, by means of which the arterial thrombus growth is controlled or influenced.
  • the measurement of the said delay time of the blood platelets is made possible, statements about, for example, the best disease risks, such as arterial tendency to thrombosis, for example via the risk of a patient's heart attack.
  • drugs can be developed for the first time that specifically affect the platelet delay Ti e.
  • the determination of the platelet delay time on fresh blood of a patient can be determined "bedside" very quickly (without anticoagulation).
  • the platelet function can be determined quickly with a high level of reproducibility and accuracy and with only a small blood volume.
  • Figure 1 is a schematic representation of a device for determining the platelet delay time
  • FIG. 2 shows a diagram to show the dependence of the aperture diameter on time, due to the occlusion by platelets
  • Figure 3 is a diagram showing the wall shear rate as a function of time
  • Figure 4 is a schematic representation of a device designed as a disposable part for performing the method according to the invention.
  • a time-dependent determination of the clogging of an aperture by platelets when blood flows through has surprisingly shown that the aperture closes in a very defined manner, namely according to the straight line shown in FIG. 2, the slope 2 dr / dt of which indicates the growth rate.
  • the time-dependent wall shear rate of the platelets which is a measure of the transport rate of the platelets
  • the wall shear rate rises sharply with time from an initially low value in the area of the aperture.
  • there is a platelet delay time which indicates the time after which the individual platelets only allow other platelets to adhere.
  • each platelet determines" when, ie after which delay or delay time, other platelets may adhere to it.
  • This platelet delay time is of the utmost importance in connection with certain health risks and with the development of medications means that the determination according to the invention of the slope dr / dt mentioned, that is to say the platelet delay time, enables specific statements to be made about health risks, for example about the risk of a patient's heart attack, and that therefore drugs can be specifically developed which affect the platelet delay time.
  • FIG. 1 shows a schematic representation of a device for determining the platelet delay time.
  • blood is drawn from a storage space (not shown in more detail), for example via a capillary 4, which has a flow resistance. stood Wc for the blood, moved through an aperture 3 of an aperture holder 2. The aperture 3 forms a flow resistance Wa for the blood.
  • equations 1 to 3 denote:
  • the pressure drop ⁇ p at the aperture 3 can also be measured, the flow resistance Wc of the capillary 4 then having to be subtracted in order to determine the growth rate.
  • the wall shear rate yw in the area of the aperture 3 can be calculated and plotted as a function of time in accordance with FIG. 3. It turns out that the transport rate of the platelets on the thrombus, which is approximately proportional to the wall shear rate, increases by a factor of 4 in the course of the measurement. During this increase, however, the growth rate of the thrombus remains exactly the same according to FIG. It follows that the individual platelets in the area of the aperture 3 determine, according to a platelet delay time, when other platelets are allowed to adhere to them. The platelet delay time thus corresponds to the time difference between the adhesion of a platelet on the wall of aperture 3 or on another platelet and the adherence of another platelet.
  • FIG. 4 shows an embodiment of the present device in a schematic representation, with blood being pressed or conveyed from a blood storage space 10 with the aid of a piston 12 through the aperture 3 into a blood collection space 14.
  • the blood storage space 10 is preferably in a cylinder.
  • Linder 16 formed in which the piston 12 is slidably disposed.
  • the piston / cylinder arrangement 12, 16 can expediently be in the form of a blood withdrawal syringe, the blood reservoir 10 being filled when blood is withdrawn from a patient's vein.
  • the front end 18 of the piston / cylinder arrangement 12, 16 can be connected to a part comprising the blood collection space 14, which is preferably designed as a disposable or disposable part, the part of which is connected to the piston / Cylinder arrangement 12, 16 connectable access opening 20 has an aperture holder 22 with the aperture 3, through which the blood passes from the blood storage space 10 into the blood collection space 14 when the piston 12 is actuated in the direction of the arrow 24.
  • the aperture 3 can be preceded by a capillary (in FIG. 1: reference number 4).
  • the disposable part can have a passage 26 which runs in or along its wall from the outside to the space upstream of the aperture 3 and which is connected to a pressure measuring device in a measuring device when measurements are being carried out.
  • An advantage here is that the disposable part for performing the present method after a blood sample, for example on a patient's bed, is connected directly to the piston / cylinder arrangement 12, 16 used for blood withdrawal and together with the piston / cylinder arrangement 12, 16 can be used in the measuring device executing the present method, which actuates the piston 12.
  • the platelet delay time is not dependent on the capillary resistance, capillary errors are not included in the measurement if a capillary is used, so that the measuring accuracy and reliability can be increased.
  • the slope of the straight line dr / dt is also determined, but not a value CT of the bleeding time is extrapolated, but rather the angle PTGA (platelet thrombus growth angle) is determined, which is between the straight line and the t-axis exists (see Figure 5).
  • PTGA platelet thrombus growth angle
  • this angle PTGA can be determined very quickly from the slope of the straight line determined at the start of a measurement using the formula given below, so that the measurement time can be relatively short and only a relatively small blood volume is required for the measurement.
  • the platelet function can, according to this variant of the present invention, in particular also with relatively long times of thrombus formation in the aperture 3, as is possible, for example, when medication is influenced, for example when taking aspirin, ie also with a small blood volume.
  • the values of the determined angles are PTGA or PTGA 'in this respect comparatively meaningful because they are not subject to as large fluctuations as the CT values determined.
  • a further variant of the present method is explained below, according to which a quality control of the determined straight line or the determined angle PTGA or PTGA 'is carried out. As already indicated above, it is determined how exactly the measured values dr / dt lie on a straight line or not. In the event of deviations from a predetermined number of values beyond a predetermined value, the corresponding measurement is classified or corrected as unusable.
  • a dotted line in FIG. 2 shows that the slope of the straight line can change during a measurement, so that the straight line has two sections of different slopes drl / dt and dr2 / dt can include, the intersection P of these sections is due to a specific thrombus formation, which is determined by a specific clinical picture. In such cases, the point P is very meaningful, which is why it is determined during the measurement.
  • FIG. 6 A further preferred embodiment for carrying out the present method is explained in more detail below in connection with FIG. 6.
  • This essentially has a piston / cylinder arrangement 10, which comprises a cylinder 30 and a piston 50.
  • the piston 50 can by a drive, not shown, in the direction of arrow 70, i. H. thus be moved in its axial direction in the cylinder 30.
  • the piston 50 preferably has the shape of a metal part polished on its outer surface, which is made in particular of stainless steel and has the shape of an elongated cylindrical rod part.
  • An O-ring seal 90 which is preferably made of a rubber material, is arranged between the outer surface of the piston 50 and the inner surface of the cylinder 30, which is preferably also made of stainless steel. Since the outer surface of the piston 50 is polished, there is extremely little friction between the seal 90 and the piston 50, so that a smooth movement of the piston 50 in the direction of the arrow 70 is ensured.
  • the piston 50 protrudes into a blood-receiving space 110, which is determined by a cup-shaped container 130, which, with its upper edge region facing the piston 50, is close to the cylinder 30 with the aid of a seal 150 is scheduled.
  • the cylinder 30 preferably has a flange part 170 which extends radially outwards and a flange part 190 which also projects radially outwards is arranged on the upper edge of the container 130, wherein the O-ring seal 150 is held between the flange parts 170 and 190 and connects them tightly together.
  • an aperture holder 230 which holds a plate 250 or the like with an aperture 270 arranged therein, a capillary 290 passing from the outside through the bottom part 210 ending shortly before the aperture 270 in a manner known per se, so that blood drawn in from a blood supply space (not shown) through the capillary 290 enters the receiving space 110 through the aperture 270 when the piston 50 moves in the direction of the arrow 70.
  • the aperture 270 can also be configured and arranged in a different way. For example, it can be formed by the end of the capillary 290 projecting into the receiving space 11.
  • a pressure measuring device P is connected to a passage 310, which is preferably located in the wall of the cylinder 30, for measuring the pressure above the blood sucked through the aperture 270 into the receiving space 110.
  • a passage 310 which is preferably located in the wall of the cylinder 30, for measuring the pressure above the blood sucked through the aperture 270 into the receiving space 110.
  • an electrical resistance can also be determined after applying a potential difference.
  • the currently existing radius of the aperture 3 can also be determined optically.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • External Artificial Organs (AREA)

Abstract

L'invention concerne un procédé servant à analyser la fonction plaquettaire du sang et comprenant les étapes suivantes : a) passage de sang ou de composants sanguins à travers une ouverture (3) ; b) détermination du rayon actif de l'ouverture (3) en fonction du temps ; c) évaluation de la variation dans le temps du rayon en tant que mesure de la fonction des globules sanguins ou de la fonction plaquettaire.
EP03749845A 2002-05-10 2003-05-09 Procede pour analyser la fonction plaquettaire du sang Withdrawn EP1504262A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10221054 2002-05-10
DE10221054A DE10221054A1 (de) 2002-05-10 2002-05-10 Verfahren zur Untersuchung der Thrombozytenfunktion des Blutes
PCT/DE2003/001491 WO2003096012A1 (fr) 2002-05-10 2003-05-09 Procede pour analyser la fonction plaquettaire du sang

Publications (1)

Publication Number Publication Date
EP1504262A1 true EP1504262A1 (fr) 2005-02-09

Family

ID=29285307

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03749845A Withdrawn EP1504262A1 (fr) 2002-05-10 2003-05-09 Procede pour analyser la fonction plaquettaire du sang

Country Status (5)

Country Link
US (2) US7915049B2 (fr)
EP (1) EP1504262A1 (fr)
JP (1) JP4402585B2 (fr)
DE (1) DE10221054A1 (fr)
WO (1) WO2003096012A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7935498B2 (en) * 2006-07-07 2011-05-03 Siemens Healthcare Diagnostics Inc. Methods for identifying patients with increased risk of an adverse cardiovascular event
US8103479B2 (en) * 2006-12-29 2012-01-24 Teradata Us, Inc. Two dimensional exponential smoothing
WO2009106047A1 (fr) * 2008-02-25 2009-09-03 Vdg-Von Der Goltz Gmbh Procédé pour déterminer la fonction plaquettaire du sang

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH478410A (de) * 1966-10-11 1969-09-15 Greiner Electronic Ag Verfahren zur Bestimmung der Blutgerinnung
US3674012A (en) 1970-04-17 1972-07-04 American Optical Corp Blood coagulation detection device
US3674013A (en) * 1970-09-30 1972-07-04 American Optical Corp Fiberoptic catheter
DE3247815C2 (de) * 1982-12-23 1985-10-17 Gustav Viktor Rudolf Prof. London Born Einrichtung zur Messung der Blutungszeit in vitro
GB8403823D0 (en) 1984-02-14 1984-03-21 Raychem Ltd Adhesive composition
DE3541057A1 (de) 1985-11-19 1987-05-21 Kratzer Michael Verfahren und einrichtung zur messung der aggregation der blutplaettchen bzw. der koagulation des blutes
EP0716744B1 (fr) * 1994-06-30 2001-11-07 Dade Behring Inc. Elements de separation poreux bioactifs
US5504011A (en) * 1994-10-21 1996-04-02 International Technidyne Corporation Portable test apparatus and associated method of performing a blood coagulation test

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO03096012A1 *

Also Published As

Publication number Publication date
JP2005532534A (ja) 2005-10-27
US20050227361A1 (en) 2005-10-13
US7915049B2 (en) 2011-03-29
US20110244508A1 (en) 2011-10-06
JP4402585B2 (ja) 2010-01-20
WO2003096012A1 (fr) 2003-11-20
DE10221054A1 (de) 2003-11-27

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