EP1504091A4 - Error-prone dna polymerase i mutants and methods for targeted random mutagenesis in continuous culture using error-prone dna polymerase i mutants - Google Patents
Error-prone dna polymerase i mutants and methods for targeted random mutagenesis in continuous culture using error-prone dna polymerase i mutantsInfo
- Publication number
- EP1504091A4 EP1504091A4 EP03736735A EP03736735A EP1504091A4 EP 1504091 A4 EP1504091 A4 EP 1504091A4 EP 03736735 A EP03736735 A EP 03736735A EP 03736735 A EP03736735 A EP 03736735A EP 1504091 A4 EP1504091 A4 EP 1504091A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- mutants
- error
- dna polymerase
- prone dna
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38494402P | 2002-05-31 | 2002-05-31 | |
US384944P | 2002-05-31 | ||
PCT/US2003/016798 WO2003102213A2 (en) | 2002-05-31 | 2003-05-29 | Error-prone dna polymerase i mutants and methods for targeted random mutagenesis in continuous culture using error-prone dna polymerase i mutants |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1504091A2 EP1504091A2 (en) | 2005-02-09 |
EP1504091A4 true EP1504091A4 (en) | 2006-04-26 |
Family
ID=29712109
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03736735A Withdrawn EP1504091A4 (en) | 2002-05-31 | 2003-05-29 | Error-prone dna polymerase i mutants and methods for targeted random mutagenesis in continuous culture using error-prone dna polymerase i mutants |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1504091A4 (en) |
JP (1) | JP2005528114A (en) |
AU (1) | AU2003237269A1 (en) |
CA (1) | CA2485203A1 (en) |
WO (1) | WO2003102213A2 (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102004005885A1 (en) * | 2004-02-05 | 2005-10-13 | Rheinische Friedrich-Wilhelms-Universität Bonn | Mutated DNA polymerases with increased mismatch discrimination |
EP1926816A1 (en) * | 2005-09-21 | 2008-06-04 | Meyer, Andreas | In vivo generation of dna, rna, peptide, and protein libraries |
WO2011116184A2 (en) | 2010-03-17 | 2011-09-22 | Cornell University | Methods and compositions for targeted mutagenesis in bacteria |
CN103492559B (en) * | 2011-04-11 | 2016-07-06 | 霍夫曼-拉罗奇有限公司 | There is the archaeal dna polymerase improving activity |
ES2716277T3 (en) | 2011-12-08 | 2019-06-11 | Hoffmann La Roche | DNA polymerases with enhanced activity |
EP2788479B1 (en) | 2011-12-08 | 2016-02-10 | Roche Diagnostics GmbH | Dna polymerases with improved activity |
CA2858264C (en) | 2011-12-08 | 2018-01-02 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
WO2017019617A1 (en) * | 2015-07-24 | 2017-02-02 | Manel Camps | System for continuous mutagenesis in vivo to facilitate directed evolution |
CN108130318B (en) * | 2018-02-28 | 2020-07-14 | 深圳市艾伟迪生物科技有限公司 | Mutant Taq DNA polymerase, kit for direct PCR amplification without nucleic acid extraction and application thereof |
CN112941155B (en) * | 2018-05-14 | 2023-07-14 | 北京艾克伦医疗科技有限公司 | DNA primer pair with stem-loop structure and application thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0655506A1 (en) * | 1994-10-17 | 1995-05-31 | President And Fellows Of Harvard College | DNA polymerases having modified nucleotide binding site for DNA sequencing |
WO1998035060A1 (en) * | 1997-02-07 | 1998-08-13 | Life Technologies, Inc. | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof |
-
2003
- 2003-05-29 EP EP03736735A patent/EP1504091A4/en not_active Withdrawn
- 2003-05-29 WO PCT/US2003/016798 patent/WO2003102213A2/en active Search and Examination
- 2003-05-29 AU AU2003237269A patent/AU2003237269A1/en not_active Abandoned
- 2003-05-29 JP JP2004510449A patent/JP2005528114A/en active Pending
- 2003-05-29 CA CA002485203A patent/CA2485203A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0655506A1 (en) * | 1994-10-17 | 1995-05-31 | President And Fellows Of Harvard College | DNA polymerases having modified nucleotide binding site for DNA sequencing |
WO1998035060A1 (en) * | 1997-02-07 | 1998-08-13 | Life Technologies, Inc. | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof |
Non-Patent Citations (10)
Title |
---|
BELL J B ET AL: "Base miscoding and strand misalignment errors by mutator Klenow polymerases with amino acid substitutions at tyrosine 766 in the O helix of the fingers subdomain", JOURNAL OF BIOLOGICAL CHEMISTRY 1997 UNITED STATES, vol. 272, no. 11, 1997, pages 7345 - 7351, XP002369420, ISSN: 0021-9258 * |
BLONDAL T ET AL: "CLONING, SEQUENCE ANALYSIS AND FUNCTIONAL CHARACTERIZATION OF DNA POLYMERASE I FROM THE THERMOPHILIC EUBACTERIUM RHODOTHERMUS MARINUS", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, ACADEMIC PRESS, US, vol. 34, no. 1, August 2001 (2001-08-01), pages 37 - 45, XP009061567, ISSN: 0885-4513 * |
CAMPS M ET AL: "When Pol I goes into high gear: Processive DNA synthesis by Pol I in the cell", CELL CYCLE 2004 UNITED STATES, vol. 3, no. 2, 2004, pages 116 - 118, XP002369425, ISSN: 1538-4101 * |
CAMPS MANEL ET AL: "Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 100, no. 17, 19 August 2003 (2003-08-19), pages 9727 - 9732, XP002369424, ISSN: 0027-8424 * |
DERBYSHIRE V ET AL: "The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.", THE EMBO JOURNAL. JAN 1991, vol. 10, no. 1, January 1991 (1991-01-01), pages 17 - 24, XP002369423, ISSN: 0261-4189 * |
KAUSHIK N ET AL: "Significance of the O-helix residues of Escherichia coli DNA polymerase I in DNA synthesis: dynamics of the dNTP binding pocket.", BIOCHEMISTRY. 4 JUN 1996, vol. 35, no. 22, 4 June 1996 (1996-06-04), pages 7256 - 7266, XP002369422, ISSN: 0006-2960 * |
PATEL P H ET AL: "Prokaryotic DNA polymerase I: evolution, structure, and ''base flipping'' mechanism for nucleotide selection", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 308, no. 5, 18 May 2001 (2001-05-18), pages 823 - 837, XP004466165, ISSN: 0022-2836 * |
SHINKAI A ET AL: "In vivo mutagenesis by Escherichia coli DNA polymerase I. Ile(709) in motif A functions in base selection.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. 14 DEC 2001, vol. 276, no. 50, 14 December 2001 (2001-12-14), pages 46759 - 46764, XP002369421, ISSN: 0021-9258 * |
SUZUKI MOTOSHI ET AL: "Thermus aquaticus DNA polymerase I mutants with altered fidelity. Interacting mutations in the O-helix", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 42, 20 October 2000 (2000-10-20), pages 32728 - 32735, XP002369419, ISSN: 0021-9258 * |
TOSAKA AKI ET AL: "O-helix mutant T664P of Thermus aquaticus DNA polymerase I: Altered catalytic properties for incorporation of incorrect nucleotides but not correct nucleotides", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 29, 20 July 2001 (2001-07-20), pages 27562 - 27567, XP002369418, ISSN: 0021-9258 * |
Also Published As
Publication number | Publication date |
---|---|
WO2003102213A3 (en) | 2004-06-17 |
CA2485203A1 (en) | 2003-12-11 |
EP1504091A2 (en) | 2005-02-09 |
AU2003237269A1 (en) | 2003-12-19 |
JP2005528114A (en) | 2005-09-22 |
WO2003102213A2 (en) | 2003-12-11 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
17P | Request for examination filed |
Effective date: 20041126 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR |
|
AX | Request for extension of the european patent |
Extension state: AL LT LV MK |
|
DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20060314 |
|
RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: LOEB, LAWRENCE, A. Inventor name: CAMPS, MANUEL |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20071201 |