EP1504091A4 - Mutants de polymerase i d'adn sujet a l'erreur et techniques de mutagenese aleatoire ciblee en culture continue utilisant ces mutants de polymerase i d'adn sujet a l'erreur - Google Patents

Mutants de polymerase i d'adn sujet a l'erreur et techniques de mutagenese aleatoire ciblee en culture continue utilisant ces mutants de polymerase i d'adn sujet a l'erreur

Info

Publication number
EP1504091A4
EP1504091A4 EP03736735A EP03736735A EP1504091A4 EP 1504091 A4 EP1504091 A4 EP 1504091A4 EP 03736735 A EP03736735 A EP 03736735A EP 03736735 A EP03736735 A EP 03736735A EP 1504091 A4 EP1504091 A4 EP 1504091A4
Authority
EP
European Patent Office
Prior art keywords
mutants
error
dna polymerase
prone dna
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03736735A
Other languages
German (de)
English (en)
Other versions
EP1504091A2 (fr
Inventor
Manuel Camps
Lawrence A Loeb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Original Assignee
University of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington filed Critical University of Washington
Publication of EP1504091A2 publication Critical patent/EP1504091A2/fr
Publication of EP1504091A4 publication Critical patent/EP1504091A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP03736735A 2002-05-31 2003-05-29 Mutants de polymerase i d'adn sujet a l'erreur et techniques de mutagenese aleatoire ciblee en culture continue utilisant ces mutants de polymerase i d'adn sujet a l'erreur Withdrawn EP1504091A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38494402P 2002-05-31 2002-05-31
US384944P 2002-05-31
PCT/US2003/016798 WO2003102213A2 (fr) 2002-05-31 2003-05-29 Mutants de polymerase i d'adn sujet a l'erreur et techniques de mutagenese aleatoire ciblee en culture continue utilisant ces mutants de polymerase i d'adn sujet a l'erreur

Publications (2)

Publication Number Publication Date
EP1504091A2 EP1504091A2 (fr) 2005-02-09
EP1504091A4 true EP1504091A4 (fr) 2006-04-26

Family

ID=29712109

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03736735A Withdrawn EP1504091A4 (fr) 2002-05-31 2003-05-29 Mutants de polymerase i d'adn sujet a l'erreur et techniques de mutagenese aleatoire ciblee en culture continue utilisant ces mutants de polymerase i d'adn sujet a l'erreur

Country Status (5)

Country Link
EP (1) EP1504091A4 (fr)
JP (1) JP2005528114A (fr)
AU (1) AU2003237269A1 (fr)
CA (1) CA2485203A1 (fr)
WO (1) WO2003102213A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004005885A1 (de) * 2004-02-05 2005-10-13 Rheinische Friedrich-Wilhelms-Universität Bonn Mutierte DNA-Polymerasen mit erhöhter Fehlpaarungs-Diskriminierung
EP1926816A1 (fr) * 2005-09-21 2008-06-04 Meyer, Andreas Génération in vivo de banques d'adn, d'arn, de peptides et de protéines
WO2011116184A2 (fr) 2010-03-17 2011-09-22 Cornell University Procédés et compositions pour mutagenèse ciblée dans des bactéries
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
CA2858264C (fr) 2011-12-08 2018-01-02 F. Hoffmann-La Roche Ag Adn polymerases presentant une activite amelioree
JP6140182B2 (ja) 2011-12-08 2017-05-31 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2013083262A1 (fr) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Adn polymérases avec activité améliorée
US10760071B2 (en) 2015-07-24 2020-09-01 The Regents Of The University Of California System for continuous mutagenesis in vivo to facilitate directed evolution
CN108130318B (zh) * 2018-02-28 2020-07-14 深圳市艾伟迪生物科技有限公司 突变型Taq DNA聚合酶、免核酸提取直接PCR扩增的试剂盒及其应用
CN112941155B (zh) * 2018-05-14 2023-07-14 北京艾克伦医疗科技有限公司 具有茎环结构的dna引物对及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0655506A1 (fr) * 1994-10-17 1995-05-31 President And Fellows Of Harvard College Polymérase d'ADN ayant les nucléotiden modifiées pour le site d'adhésion de l'ADN
WO1998035060A1 (fr) * 1997-02-07 1998-08-13 Life Technologies, Inc. Polymerases pour l'analyse et le typage de fragments d'acide nucleique polymorphes et leurs utilisations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0655506A1 (fr) * 1994-10-17 1995-05-31 President And Fellows Of Harvard College Polymérase d'ADN ayant les nucléotiden modifiées pour le site d'adhésion de l'ADN
WO1998035060A1 (fr) * 1997-02-07 1998-08-13 Life Technologies, Inc. Polymerases pour l'analyse et le typage de fragments d'acide nucleique polymorphes et leurs utilisations

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BELL J B ET AL: "Base miscoding and strand misalignment errors by mutator Klenow polymerases with amino acid substitutions at tyrosine 766 in the O helix of the fingers subdomain", JOURNAL OF BIOLOGICAL CHEMISTRY 1997 UNITED STATES, vol. 272, no. 11, 1997, pages 7345 - 7351, XP002369420, ISSN: 0021-9258 *
BLONDAL T ET AL: "CLONING, SEQUENCE ANALYSIS AND FUNCTIONAL CHARACTERIZATION OF DNA POLYMERASE I FROM THE THERMOPHILIC EUBACTERIUM RHODOTHERMUS MARINUS", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, ACADEMIC PRESS, US, vol. 34, no. 1, August 2001 (2001-08-01), pages 37 - 45, XP009061567, ISSN: 0885-4513 *
CAMPS M ET AL: "When Pol I goes into high gear: Processive DNA synthesis by Pol I in the cell", CELL CYCLE 2004 UNITED STATES, vol. 3, no. 2, 2004, pages 116 - 118, XP002369425, ISSN: 1538-4101 *
CAMPS MANEL ET AL: "Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 100, no. 17, 19 August 2003 (2003-08-19), pages 9727 - 9732, XP002369424, ISSN: 0027-8424 *
DERBYSHIRE V ET AL: "The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.", THE EMBO JOURNAL. JAN 1991, vol. 10, no. 1, January 1991 (1991-01-01), pages 17 - 24, XP002369423, ISSN: 0261-4189 *
KAUSHIK N ET AL: "Significance of the O-helix residues of Escherichia coli DNA polymerase I in DNA synthesis: dynamics of the dNTP binding pocket.", BIOCHEMISTRY. 4 JUN 1996, vol. 35, no. 22, 4 June 1996 (1996-06-04), pages 7256 - 7266, XP002369422, ISSN: 0006-2960 *
PATEL P H ET AL: "Prokaryotic DNA polymerase I: evolution, structure, and ''base flipping'' mechanism for nucleotide selection", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 308, no. 5, 18 May 2001 (2001-05-18), pages 823 - 837, XP004466165, ISSN: 0022-2836 *
SHINKAI A ET AL: "In vivo mutagenesis by Escherichia coli DNA polymerase I. Ile(709) in motif A functions in base selection.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. 14 DEC 2001, vol. 276, no. 50, 14 December 2001 (2001-12-14), pages 46759 - 46764, XP002369421, ISSN: 0021-9258 *
SUZUKI MOTOSHI ET AL: "Thermus aquaticus DNA polymerase I mutants with altered fidelity. Interacting mutations in the O-helix", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 42, 20 October 2000 (2000-10-20), pages 32728 - 32735, XP002369419, ISSN: 0021-9258 *
TOSAKA AKI ET AL: "O-helix mutant T664P of Thermus aquaticus DNA polymerase I: Altered catalytic properties for incorporation of incorrect nucleotides but not correct nucleotides", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 29, 20 July 2001 (2001-07-20), pages 27562 - 27567, XP002369418, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
AU2003237269A1 (en) 2003-12-19
CA2485203A1 (fr) 2003-12-11
WO2003102213A2 (fr) 2003-12-11
WO2003102213A3 (fr) 2004-06-17
EP1504091A2 (fr) 2005-02-09
JP2005528114A (ja) 2005-09-22

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