EP1463821A4 - In increasing gene transfer efficiency by pre-incubation with endothelial cells - Google Patents

In increasing gene transfer efficiency by pre-incubation with endothelial cells

Info

Publication number
EP1463821A4
EP1463821A4 EP02795952A EP02795952A EP1463821A4 EP 1463821 A4 EP1463821 A4 EP 1463821A4 EP 02795952 A EP02795952 A EP 02795952A EP 02795952 A EP02795952 A EP 02795952A EP 1463821 A4 EP1463821 A4 EP 1463821A4
Authority
EP
European Patent Office
Prior art keywords
incubation
endothelial cells
gene transfer
transfer efficiency
increasing gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02795952A
Other languages
German (de)
French (fr)
Other versions
EP1463821A1 (en
Inventor
John P Chute
Abha A Saini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
US Department of Navy
Original Assignee
US Department of Health and Human Services
US Department of Navy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Health and Human Services, US Department of Navy filed Critical US Department of Health and Human Services
Publication of EP1463821A1 publication Critical patent/EP1463821A1/en
Publication of EP1463821A4 publication Critical patent/EP1463821A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
EP02795952A 2001-12-21 2002-12-20 In increasing gene transfer efficiency by pre-incubation with endothelial cells Withdrawn EP1463821A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34186801P 2001-12-21 2001-12-21
US341868P 2001-12-21
PCT/US2002/040719 WO2003060138A1 (en) 2001-12-21 2002-12-20 In increasing gene transfer efficiency by pre-incubation with endothelial cells

Publications (2)

Publication Number Publication Date
EP1463821A1 EP1463821A1 (en) 2004-10-06
EP1463821A4 true EP1463821A4 (en) 2006-05-31

Family

ID=23339354

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02795952A Withdrawn EP1463821A4 (en) 2001-12-21 2002-12-20 In increasing gene transfer efficiency by pre-incubation with endothelial cells

Country Status (4)

Country Link
EP (1) EP1463821A4 (en)
JP (1) JP2005514071A (en)
AU (1) AU2002360674A1 (en)
WO (1) WO2003060138A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5174656B2 (en) * 2005-04-21 2013-04-03 マサチューセッツ インスティテュート オブ テクノロジー Materials and methods for modifying an immune response against exogenous and endogenous immunogens, including syngeneic or non-syngeneic cells, tissues or organs
CN101735979B (en) * 2009-12-31 2014-04-30 浙江中赢控股集团有限公司 Method for in vitro amplification of hemopoietic stem cells and precursor cells
EP3613859A1 (en) * 2013-10-24 2020-02-26 Ospedale San Raffaele S.r.l. Method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5811297A (en) * 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages
EP1294857A2 (en) * 2000-06-09 2003-03-26 Universitätsklinikum Charite Medizinische Fakultät der Humboldt-Universität zu Berlin Genetically modified t-cells, method for producing them and use thereof
WO2002078449A2 (en) * 2001-04-02 2002-10-10 Advanced Cell Technology, Inc. Method for facilitating the production of differentiated cell types and tissues from embryonic and adult pluripotent and multipotent cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHU P ET AL: "RETROVIRUS-MEDIATED GENE TRANSFER INTO HUMAN HEMATOPOIETIC STEM CELLS", JOURNAL OF MOLECULAR MEDICINE, SPRINGER VERLAG, DE, vol. 76, 1998, pages 184 - 192, XP002939375, ISSN: 0946-2716 *
CHUTE J P ET AL: "A COMPARATIVE STUDY OF THE CELL CYCLE STATUS AND PRIMITIVE CELL ADHESION MOLECULE PROFILE OF HUMAN CD34+CELLS CULTURED IN STROMA-FREE VERSUS PORCINE MICROVASCULAR ENDOTHELIAL CELL CULTURES", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 27, 1999, pages 370 - 379, XP000929912, ISSN: 0301-472X *
CHUTE J P ET AL: "Preincubation with endothelial cell monolayers increases gene transfer efficiency into human bone marrow CD34+CD38-progenitor cells", HUMAN GENE THERAPY, vol. 11, 10 December 2000 (2000-12-10), pages 2515 - 2528, XP002963382, ISSN: 1043-0342 *
ROSLER E ET AL: "Cocultivation of umbilical cord blood cells with endothelial cells leads to extensive amplification of competent CD34+CD38-cells", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 28, 2000, pages 841 - 852, XP002963383, ISSN: 0301-472X *
See also references of WO03060138A1 *
ZIEGLER B L ET AL: "EXPANSION OF STEM AND PROGENITOR CELLS", CURRENT OPINION IN HEMATOLOGY, RAPID SCIENCE PUBLISHERS, PHILADELPHIA, PA, US, vol. 5, no. 6, November 1998 (1998-11-01), pages 434 - 440, XP001009708, ISSN: 1065-6251 *

Also Published As

Publication number Publication date
AU2002360674A1 (en) 2003-07-30
WO2003060138A1 (en) 2003-07-24
JP2005514071A (en) 2005-05-19
EP1463821A1 (en) 2004-10-06

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 20040705

AK Designated contracting states

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Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO

RIN1 Information on inventor provided before grant (corrected)

Inventor name: SAINI, ABHA, A.

Inventor name: CHUTE, JOHN, P.

A4 Supplementary search report drawn up and despatched

Effective date: 20060420

17Q First examination report despatched

Effective date: 20061211

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20070424