EP1364711A2 - Pipettenspitze - Google Patents
Pipettenspitze Download PDFInfo
- Publication number
- EP1364711A2 EP1364711A2 EP03008297A EP03008297A EP1364711A2 EP 1364711 A2 EP1364711 A2 EP 1364711A2 EP 03008297 A EP03008297 A EP 03008297A EP 03008297 A EP03008297 A EP 03008297A EP 1364711 A2 EP1364711 A2 EP 1364711A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- pipette tip
- tubular body
- sealing lip
- opening
- tip according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
- B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
Definitions
- the invention relates to a pipette tip.
- Pipette tips are used together with pipetting devices for dispensing Liquids used.
- Pipette tips have an elongated, tubular body, one at one end and one at the other Push-on opening for pushing onto a receiving shaft of the pipetting device Has.
- the shank is mostly conical. But are also known cylindrical shafts. Furthermore conical or cylindrical receiving shafts, the circumferential beads or the like to reinforce the sealing or clamping effect to have.
- the pipetting device comprises a gas displacement device, which is usually as Piston-cylinder unit is executed.
- the gas displacement device is with a through opening of the receiving shaft connected.
- the pipette tip will by pressing the receiving shaft into the push-on opening on the pipetting device fixed.
- a gas column is displaced to contain liquid aspirate into or out of the pipette tip inserted on the receiving shaft to expel this.
- the gas column is usually an air column. Will the gas column by a certain amount of liquid is moved through the pipette tip the pipetting opening is sucked into the tubular body. By moving the Gas column to the pipette tip, the amount of liquid from the tubular Body discharged through the pipetting opening. Using one on the top of the Ejector acting pipette tip, the pipette tip of the receiving shaft pulled the trigger.
- the pipetting device can be a handheld pipette or a dosing station, whereby the gas displacement device can be manual or motor-operated.
- the plugging on and dropping the pipette tip can also be manual or motor driven happen.
- the pipette tip To avoid incorrect pipetting, the pipette tip must be sealed on the Be fixed to the receiving shaft. In addition, the forces for attaching the Pipette tip on and ejecting the pipette tip from the receiving shaft not be too high.
- the fixation of pipette tips on a receiving shaft is very sensitive to dimensional deviations. Deviations in dimensions of the receiving shaft or the pipette tip, for example with regard to the Cone angle and / or the cone diameter lead to high in one extreme Absorption and ejection forces. At the other extreme, the pipette tip doesn't stop the receiving shaft or the connection between the pipette tip and the receiving shaft is leaking. In the case of receptacles for pipette tips with additional elastic sealing elements (e.g. molded parts, O-rings) are strong Subject to wear.
- Pipette tips are already known, which attach to the opening on the inner circumference of the tubular body one or more circumferential sealing beads for control have the sealing and clamping function (US 6 168 761 B1, US 6 248 295 B1). Tolerance compensation is due to the considerable deformations and forces only possible to a limited extent in the sealing area.
- Pipette tips are also known in which the tubular body has a Wall thickness weakening to an elastic expansion and adjustment to promote the receiving shaft (US 4,961,350, US 4,072,330). Another reduction tolerance sensitivity is also desirable in this respect.
- the object of the invention is to provide a pipette tip create an adequate seal against a shank while reducing the push-on and ejection forces and with less tolerance sensitivity reached.
- the first variant of the pipette tip according to the invention has an elongated one.
- tubular Body with a pipetting opening at one end and the other End of a push-on opening for pushing onto a receiving shaft of a pipetting device has that of an axially directed and inwardly inclined, at the Base connected to the tubular body, rotating and by plugging is delimited on the receiving shaft elastically expandable sealing lip.
- the pipette tip is conventionally received by the receiving shaft and aligned.
- the sealing and fastening function is essentially from the Sealing lip taken over when the pipette tip is attached to the receiving shaft is elastically expanded. This is due to the inclination of the sealing lip particularly large elastic deformation on the inside (i.e. towards the pipette axis) reached the push-on opening, which brings about an improved tolerance compensation. Due to the inclination of the sealing lip inwards, it becomes more elastic Execution of the sealing lip achieved quite significant elastic deformations effect a tolerance compensation, so that the desired seal at the same time secure seat of the pipette tip on the receiving shaft is guaranteed.
- the expansion of the sealing lip takes place increasing depth of penetration of the slip-on cone and only reached at the end of the Slip movement their maximum value. This makes it easier to attach.
- Therefor can also be achieved by coordinating the pipette tip and the intake cone be that the sealing lip only elastically expanded in the course of the push-on movement if the receiving cone has already penetrated the plug-in opening is.
- the sealing lip is attached to a cylindrical mounting shaft already widened at the beginning of the slip-on movement, which is indicated by a conical Insertion opening at the end of the sealing lip can be controlled.
- the considerable elastic expansion of the sealing lip ensures that tolerances the seal does not impair and the push-on and ejection forces stay low.
- the sealing lip is covered by a circumferential slot separated a jacket portion of the tubular body.
- the jacket section can be used in particular to create a dropper for the pipetting device. It can also limit the deformation of the sealing lip and thus the seat of the pipette tip stabilize on the cone.
- the second variant of the pipette tip according to the invention has an elongated, tubular body which has a pipetting opening at one end and at the at the other end a push-on opening for pushing onto a holding shaft a pipetting device, which is from an axially directed, at the base with the tubular body connected, revolving, by plugging onto the receiving cone elastically expandable sealing lip is delimited by a circumferential Slit is separated from a jacket portion of the tubular body.
- the circumferential slot increases Elasticity in the area of the sealing lip causes an adequate seal against a receiving shaft while reducing the push-on and ejection forces has lower tolerance sensitivity.
- the jacket section can in particular serve the installation of a dropper of the pipetting device. He can too limit the deformation of the sealing lip and thus the seat of the pipette tip stabilize the receiving cone.
- the sealing lip can in particular be aligned parallel to the pipette axis or away from the pipette axis be inclined or inclined towards the pipette axis.
- the jacket section has a plug-in opening circumferential waistband. This can in particular hold the pipette tip serve at the edge of a receiving opening of a tip holder. When plugging in the pipette tip with the collar can adhere to a receiving shaft support the lace holder.
- the sealing lip has an inside at its free end circumferential bead, which is particularly good due to the increased surface pressure sealingly rests on the jacket of the receiving shaft.
- the sealing lip has its base on the largest inside diameter of the tubular body, whereby the insertion of the receiving shaft in the push-on opening is facilitated.
- the pipette tip is made in one piece.
- the pipette tip is made of plastic. Basically, it can be made from several different plastics, for example from one particularly elastic plastic in the area of the sealing lip and the rest a less elastic plastic.
- the pipette tip consists of only one plastic (e.g. made of polypropylene or polyethylene).
- a special elasticity of the sealing lip can be ensured in particular in that this has a lower Has wall thickness than the tubular body.
- the sealing lip preferably has one Wall thickness of a maximum of about 50% of the tubular body.
- the pipette tip has a limit stop the immersion depth of the receiving shaft.
- the stop can, for example be formed by an internal step in the pipette tip below the sealing lip, against which the stop abuts with its end face, causing the immersion depth of the Admission is limited.
- a pipette tip 1 has an elongated, tubular body 2, which in the example a slightly conical main section 2 'and a more conical end section 2 ".
- the tubular body 2 has a pipetting opening 3 and at one end the other end a push-on opening 4 for pushing onto a receiving cone a pipetting device.
- the push-on opening 4 is delimited by a sealing lip 5, which starts from it Base, on which it is connected to the tubular body 2, axially to the push-on opening 4 is directed, with an inclination inwards to the central axis of the tubular Body 2.
- the sealing lip 5 has a circumferential inside at its free end Bead 6.
- the sealing lip 5 rests on the maximum inner diameter of the tubular body 2 on. It is through an axially directed slot 7 from a jacket region 2 '' 'of separate tubular body 2, which in turn has a circumferential collar 8.
- the entire pipette tip 1 is made in one piece from a single plastic material injected.
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
- Fig. 1
- die Pipettenspitze in einem Längsschnitt;
- Fig. 2
- vergrößerter Teilschnitt II derselben Pipettenspitze.
Claims (11)
- Pipettenspitze mit einem länglichen, rohrförmigen Körper (2), der an dem einen Ende eine Pipettieröffnung (3) und an dem anderen Ende eine Aufstecköffnung (4) zum Aufstecken auf einen Aufnahmeschaft einer Pipettiervorrichtung hat, die von einer axial gerichteten und nach innen geneigten, an der Basis mit dem rohrförmigen Körper (2) verbundenen, umlaufenden, durch Aufstecken auf den Aufnahmekonus elastisch aufweitbaren Dichtlippe (5) umgrenzt ist.
- Pipettenspitze nach Anspruch 1, bei der die Dichtlippe (5) durch einen umlaufenden Schlitz (7) von einem Mantelabschnitt (2''') des rohrförmigen Körpers (2) getrennt ist.
- Pipettenspitze mit einem länglichen, rohrförmigen Körper (2), der an dem einen Ende eine Pipettieröffnung (3) und an dem anderen Ende eine Aufstecköffnung (4) zum Aufstecken auf einen Aufnahmeschaft einer Pipettiervorrichtung hat, die von einer axial gerichteten, an der Basis mit dem rohrförmigen Körper (2) verbundenen, umlaufenden, durch Aufstecken auf den Aufnahmekonus elastisch aufweitbaren Dichtlippe (5) umgrenzt ist, die durch einen umlaufenden Schlitz (7) von einem Mantelabschnitt (2''') des rohrförmigen Körpers (2) getrennt ist.
- Pipettenspitze nach Anspruch 2 oder 3, bei der der Mantelabschnitt (2''') einen um die Aufstecköffnung (4) umlaufenden Bund (8) aufweist.
- Pipettenspitze nach einem der Ansprüche 1 bis 4, bei der die Dichtlippe (5) an ihrem freien Ende innen einen umlaufenden Wulst (6) aufweist.
- Pipettenspitze nach einem der Ansprüche 1 bis 5, bei der die Dichtlippe (5) ihre Basis am größten Innendurchmesser des rohrförmigen Körpers (2) hat.
- Pipettenspitze nach einem der Ansprüche 1 bis 6, die einteilig hergestellt ist.
- Pipettenspitze nach einem der Ansprüche 1 bis 7, die aus Kunststoff hergestellt ist.
- Pipettenspitze nach Anspruch 8, die aus nur einem Kunststoff besteht.
- Pipettenspitze nach einem der Ansprüche 1 bis 9, bei der die Dichtlippe (5) eine geringere Wandstärke als der rohrförmige Körper (2) aufweist.
- Pipettenspitze nach einem der Ansprüche 1 bis 10, die einen Anschlag zum Begrenzen der Eintauchtiefe des Aufnahmeschaftes hat.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10222511A DE10222511B4 (de) | 2002-05-22 | 2002-05-22 | Pipettenspitze |
| DE10222511 | 2002-05-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP1364711A2 true EP1364711A2 (de) | 2003-11-26 |
| EP1364711A3 EP1364711A3 (de) | 2005-01-12 |
Family
ID=29285618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP03008297A Withdrawn EP1364711A3 (de) | 2002-05-22 | 2003-04-10 | Pipettenspitze |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20030219359A1 (de) |
| EP (1) | EP1364711A3 (de) |
| DE (1) | DE10222511B4 (de) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110052300A (zh) * | 2019-05-20 | 2019-07-26 | 江西省农业科学院土壤肥料与资源环境研究所 | 一种可伸缩式称量器 |
| CN114929390A (zh) * | 2020-01-15 | 2022-08-19 | 珀金埃尔默保健科学公司 | 移液器尖端和包括其的方法及系统 |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19917375C2 (de) * | 1999-04-16 | 2001-09-27 | Hamilton Bonaduz Ag Bonaduz | Pipettiereinheit |
| US7335337B1 (en) * | 2001-09-11 | 2008-02-26 | Smith James C | Ergonomic pipette tip and adapters |
| US7641859B2 (en) * | 2004-02-11 | 2010-01-05 | Matrix Technologies Corporation | Pipette tip mounting and ejection assembly and associated pipette tip |
| EP1862219B1 (de) | 2006-05-29 | 2018-02-07 | Qiagen GmbH | Vorrichtung zur halterung von pipettenspitzen sowie pipettiervorrichtung |
| US7662343B2 (en) * | 2006-10-24 | 2010-02-16 | Viaflo Corporation | Locking pipette tip and mounting shaft |
| US7662344B2 (en) * | 2006-10-24 | 2010-02-16 | Viaflo Corporation | Locking pipette tip and mounting shaft |
| EP2138234A1 (de) * | 2008-06-24 | 2009-12-30 | F. Hoffmann-Roche AG | Flexible Nahtstelle für Einwegspitzen |
| US8277757B2 (en) * | 2009-09-29 | 2012-10-02 | Integra Biosciences Corp. | Pipette tip mounting shaft |
| EP4541463A1 (de) | 2023-10-16 | 2025-04-23 | Tecan Trading AG | Pipettenspitze mit äusserem dichtkragen |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2526296C3 (de) * | 1975-06-12 | 1978-05-18 | Eppendorf Geraetebau Netheler + Hinz Gmbh, 2000 Hamburg | Aufsteckbare Pipettenspitze |
| US4212204A (en) * | 1979-04-26 | 1980-07-15 | St Amand Elmer F | Pipette and method of making same |
| FI65923C (fi) * | 1980-02-15 | 1984-08-10 | Suovaniemi Finnpipette | Spetsbehaollarelement |
| FI843976L (fi) * | 1983-10-13 | 1985-04-14 | Cetus Corp | Utbytbar pipettspets. |
| JPS62144755A (ja) * | 1985-12-20 | 1987-06-27 | Fuji Photo Film Co Ltd | 再吸引防止ピペツト |
| US4824641A (en) * | 1986-06-20 | 1989-04-25 | Cetus Corporation | Carousel and tip |
| DE3824767A1 (de) * | 1988-07-21 | 1990-02-01 | Eppendorf Geraetebau Netheler | Aufsteckbare pipettenspitze in form eines entsprechend einem aufsteckkopfstueck, insbesondere konus einer pipette, wenigstens abschnittsweise ausgefuehrten gefaesses |
| JPH03131351A (ja) * | 1989-10-16 | 1991-06-04 | Fuji Photo Film Co Ltd | 撥水処理されたピペツトチツプ |
| US5200151A (en) * | 1990-05-21 | 1993-04-06 | P B Diagnostic Systems, Inc. | Fluid dispensing system having a pipette assembly with preset tip locator |
| US5496523A (en) * | 1994-05-06 | 1996-03-05 | Sorenson Bioscience | Filtered micropipette tip for high/low volume pipettors |
| CA2185292A1 (en) * | 1995-09-15 | 1997-03-16 | James C. Smith | Positive displacement liquid drawing and dispensing apparatus and method |
| DE19626234A1 (de) * | 1996-06-29 | 1998-01-02 | Innova Gmbh | Vorrichtung zur kontaminationsfreien Zu- und Abfuhr von Flüssigkeiten |
| FI105783B (fi) * | 1998-08-26 | 2000-10-13 | Biohit Oyj | Menetelmä ja laite nesteen annostelemiseksi |
| DE99958813T1 (de) * | 1998-11-06 | 2006-02-09 | Rainin Instrument Llc., Oakland | Pipette mit verbesserter Pipettenspitze und verbessertem Befestigungsschaft |
| US6168761B1 (en) * | 1998-11-06 | 2001-01-02 | Rainin Instrument Co., Inc. | Pipette with improved pipette tip and mounting shaft |
| US6197259B1 (en) * | 1998-11-06 | 2001-03-06 | Rainin Instrument Co., Inc. | Easy eject pipette tip |
| US6248295B1 (en) * | 1998-11-06 | 2001-06-19 | Rainin Instrument Co., Inc. | Pipette with improved pipette tip and mounting shaft combination |
| US20030012699A1 (en) * | 1998-11-18 | 2003-01-16 | Thomas Moore | Simultaneous handling of magnetic beads in a two-dimensional arrangement |
| US6566145B2 (en) * | 2000-02-09 | 2003-05-20 | William E Brewer | Disposable pipette extraction |
| US6641993B1 (en) * | 2000-02-22 | 2003-11-04 | Ortho Clinical Diagnostics, Inc. | Aspirating and mixing of liquids within a probe tip |
| JP4076048B2 (ja) * | 2000-09-22 | 2008-04-16 | 富士フイルム株式会社 | 定量吸引装置 |
| US6596240B2 (en) * | 2001-01-12 | 2003-07-22 | Porex Corporation | Pipette tip for easy mounting and ejecting from a pipette |
| US6913933B2 (en) * | 2001-12-03 | 2005-07-05 | Ortho-Clinical Diagnostics, Inc. | Fluid dispensing algorithm for a variable speed pump driven metering system |
| JP3648487B2 (ja) * | 2002-03-01 | 2005-05-18 | アロカ株式会社 | 分注装置用ノズルチップ |
| US6955077B2 (en) * | 2002-05-09 | 2005-10-18 | Quality Scientific Plastics, Inc. | Pipette tip with an internal sleeve and method for forming same |
| JP3990965B2 (ja) * | 2002-10-04 | 2007-10-17 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
-
2002
- 2002-05-22 DE DE10222511A patent/DE10222511B4/de not_active Expired - Lifetime
-
2003
- 2003-04-10 EP EP03008297A patent/EP1364711A3/de not_active Withdrawn
- 2003-05-06 US US10/430,145 patent/US20030219359A1/en not_active Abandoned
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110052300A (zh) * | 2019-05-20 | 2019-07-26 | 江西省农业科学院土壤肥料与资源环境研究所 | 一种可伸缩式称量器 |
| CN114929390A (zh) * | 2020-01-15 | 2022-08-19 | 珀金埃尔默保健科学公司 | 移液器尖端和包括其的方法及系统 |
| CN114929390B (zh) * | 2020-01-15 | 2024-05-10 | 珀金埃尔默保健科学公司 | 移液器尖端和包括其的方法及系统 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE10222511A1 (de) | 2003-12-04 |
| US20030219359A1 (en) | 2003-11-27 |
| EP1364711A3 (de) | 2005-01-12 |
| DE10222511B4 (de) | 2005-03-17 |
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