EP1194574A1 - HOCHEFFIZIENTE TRANSFEKTION MITTELS NIEDRIGER ELEKTRISCHER FELDSTäRKE UND LANGER PULSDAUER - Google Patents
HOCHEFFIZIENTE TRANSFEKTION MITTELS NIEDRIGER ELEKTRISCHER FELDSTäRKE UND LANGER PULSDAUERInfo
- Publication number
- EP1194574A1 EP1194574A1 EP99928868A EP99928868A EP1194574A1 EP 1194574 A1 EP1194574 A1 EP 1194574A1 EP 99928868 A EP99928868 A EP 99928868A EP 99928868 A EP99928868 A EP 99928868A EP 1194574 A1 EP1194574 A1 EP 1194574A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- cells
- cell
- nucleic acid
- impulse
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07010185A EP1829973A3 (de) | 1999-06-25 | 1999-06-25 | Hocheffiziente Durchtrennung auf der Basis niedriger Stärke des elektrischen Feldes und niedriger Impulslänge |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1999/014447 WO2001000856A1 (en) | 1999-06-25 | 1999-06-25 | High efficiency transfection based on low electric field strength, long pulse length |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP07010185A Division EP1829973A3 (de) | 1999-06-25 | 1999-06-25 | Hocheffiziente Durchtrennung auf der Basis niedriger Stärke des elektrischen Feldes und niedriger Impulslänge |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1194574A1 true EP1194574A1 (de) | 2002-04-10 |
EP1194574A4 EP1194574A4 (de) | 2003-04-02 |
Family
ID=22273056
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP07010185A Withdrawn EP1829973A3 (de) | 1999-06-25 | 1999-06-25 | Hocheffiziente Durchtrennung auf der Basis niedriger Stärke des elektrischen Feldes und niedriger Impulslänge |
EP99928868A Ceased EP1194574A4 (de) | 1999-06-25 | 1999-06-25 | HOCHEFFIZIENTE TRANSFEKTION MITTELS NIEDRIGER ELEKTRISCHER FELDSTäRKE UND LANGER PULSDAUER |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP07010185A Withdrawn EP1829973A3 (de) | 1999-06-25 | 1999-06-25 | Hocheffiziente Durchtrennung auf der Basis niedriger Stärke des elektrischen Feldes und niedriger Impulslänge |
Country Status (4)
Country | Link |
---|---|
EP (2) | EP1829973A3 (de) |
AU (1) | AU783432B2 (de) |
CA (1) | CA2377565A1 (de) |
WO (1) | WO2001000856A1 (de) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003016860A2 (en) | 2001-08-14 | 2003-02-27 | Washington University In St. Louis | Systems and methods for screening pharmaceutical chemicals |
US20130177988A1 (en) * | 2011-11-17 | 2013-07-11 | Brigham Young University | Cytoplasm to organelle delivery system and associated methods |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997007826A1 (en) * | 1995-08-29 | 1997-03-06 | Cbr Laboratories, Inc. | In vivo electroporation of cells |
WO1999001158A1 (fr) * | 1997-06-30 | 1999-01-14 | Rhone-Poulenc Rorer S.A. | Amelioration du transfert d'acide nucleique dans le muscle strie et combinaison permettant la mise en oeuvre du procede |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2798459B2 (ja) * | 1988-01-21 | 1998-09-17 | マサチユセツツ・インスチチユート・オブ・テクノロジー | エレクトロポレーションを利用した診断装置及び分子の組織内移動装置 |
US5944710A (en) * | 1996-06-24 | 1999-08-31 | Genetronics, Inc. | Electroporation-mediated intravascular delivery |
BR9810372A (pt) * | 1997-06-30 | 2000-09-05 | Rhone Poulenc Rorer Sa | Processo de transferência de ácido nucléico para o interior de células de organismos eucarióticos pluricelulares in vivo, composição, ácido nucléico e campo elétrico, e, produto de combinação |
-
1999
- 1999-06-25 CA CA002377565A patent/CA2377565A1/en not_active Abandoned
- 1999-06-25 AU AU45842/99A patent/AU783432B2/en not_active Ceased
- 1999-06-25 WO PCT/US1999/014447 patent/WO2001000856A1/en not_active Application Discontinuation
- 1999-06-25 EP EP07010185A patent/EP1829973A3/de not_active Withdrawn
- 1999-06-25 EP EP99928868A patent/EP1194574A4/de not_active Ceased
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997007826A1 (en) * | 1995-08-29 | 1997-03-06 | Cbr Laboratories, Inc. | In vivo electroporation of cells |
WO1999001158A1 (fr) * | 1997-06-30 | 1999-01-14 | Rhone-Poulenc Rorer S.A. | Amelioration du transfert d'acide nucleique dans le muscle strie et combinaison permettant la mise en oeuvre du procede |
Non-Patent Citations (2)
Title |
---|
AIHARA H ET AL: "GENE TRANSFER INTO MUSCLE BY ELECTROPORATION IN VIVO" NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, no. 9, September 1998 (1998-09), pages 867-870, XP001061636 ISSN: 1087-0156 * |
See also references of WO0100856A1 * |
Also Published As
Publication number | Publication date |
---|---|
AU783432B2 (en) | 2005-10-27 |
EP1829973A3 (de) | 2008-01-09 |
WO2001000856A1 (en) | 2001-01-04 |
EP1194574A4 (de) | 2003-04-02 |
AU4584299A (en) | 2001-01-31 |
EP1829973A2 (de) | 2007-09-05 |
CA2377565A1 (en) | 2001-01-04 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20020118 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20030218 |
|
17Q | First examination report despatched |
Effective date: 20050613 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED |
|
18R | Application refused |
Effective date: 20070524 |