EP1179095A4 - Methods for producing modified nucleic acid molecules - Google Patents

Methods for producing modified nucleic acid molecules

Info

Publication number
EP1179095A4
EP1179095A4 EP00937524A EP00937524A EP1179095A4 EP 1179095 A4 EP1179095 A4 EP 1179095A4 EP 00937524 A EP00937524 A EP 00937524A EP 00937524 A EP00937524 A EP 00937524A EP 1179095 A4 EP1179095 A4 EP 1179095A4
Authority
EP
European Patent Office
Prior art keywords
methods
nucleic acid
acid molecules
modified nucleic
producing modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00937524A
Other languages
German (de)
French (fr)
Other versions
EP1179095A1 (en
Inventor
Alison F Chalker
James Yigong Ge
David J Holmes
Robert D Lunsford
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Ltd
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Ltd
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Ltd, SmithKline Beecham Corp filed Critical SmithKline Beecham Ltd
Publication of EP1179095A1 publication Critical patent/EP1179095A1/en
Publication of EP1179095A4 publication Critical patent/EP1179095A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pulmonology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP00937524A 1999-05-10 2000-05-04 Methods for producing modified nucleic acid molecules Withdrawn EP1179095A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13339199P 1999-05-10 1999-05-10
US133391P 1999-05-10
PCT/US2000/012103 WO2000068428A1 (en) 1999-05-10 2000-05-04 Methods for producing modified nucleic acid molecules

Publications (2)

Publication Number Publication Date
EP1179095A1 EP1179095A1 (en) 2002-02-13
EP1179095A4 true EP1179095A4 (en) 2004-10-13

Family

ID=22458389

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00937524A Withdrawn EP1179095A4 (en) 1999-05-10 2000-05-04 Methods for producing modified nucleic acid molecules

Country Status (3)

Country Link
EP (1) EP1179095A4 (en)
JP (1) JP2002543801A (en)
WO (1) WO2000068428A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006114834A1 (en) * 2005-03-31 2006-11-02 Hokkaido Technology Licensing Office Co., Ltd. Method of constructing vector for introducing point-specific functional group

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033207A1 (en) * 1995-04-18 1996-10-24 Glaxo Group Limited End-complementary polymerase reaction
WO1999016904A1 (en) * 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033207A1 (en) * 1995-04-18 1996-10-24 Glaxo Group Limited End-complementary polymerase reaction
WO1999016904A1 (en) * 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CLAVERYS J-P ET AL: "Construction and evaluation of new drug-resistance cassettes for gene disruption mutagenesis in Streptococcus pneumoniae, using an ami test platform", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 164, no. 1, 1995, pages 123 - 128, XP004041930, ISSN: 0378-1119 *
HORTON R M ET AL: "Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 77, 1989, pages 61 - 68, XP002090392, ISSN: 0378-1119 *
NIKAWA J ET AL: "PCR- AND LIGATION-MEDIATED SYNTHESIS OF MARKER CASSETTES WITH LONG FLANKING HOMOLOGY REGIONS FOR GENE DISRUPTION IN SACCHAROMYCES CEREVISIAE", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 3, 1998, pages 860 - 861, XP002931027, ISSN: 0305-1048 *
See also references of WO0068428A1 *

Also Published As

Publication number Publication date
EP1179095A1 (en) 2002-02-13
WO2000068428A1 (en) 2000-11-16
JP2002543801A (en) 2002-12-24

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Legal Events

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