EP1131078A4 - Compositions and methods for recombinational cloning of nucleic acid molecules - Google Patents
Compositions and methods for recombinational cloning of nucleic acid moleculesInfo
- Publication number
- EP1131078A4 EP1131078A4 EP99960316A EP99960316A EP1131078A4 EP 1131078 A4 EP1131078 A4 EP 1131078A4 EP 99960316 A EP99960316 A EP 99960316A EP 99960316 A EP99960316 A EP 99960316A EP 1131078 A4 EP1131078 A4 EP 1131078A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- compositions
- methods
- nucleic acid
- acid molecules
- recombinational cloning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10832498P | 1998-11-13 | 1998-11-13 | |
US108324P | 1998-11-13 | ||
PCT/US1999/026871 WO2000029000A1 (en) | 1998-11-13 | 1999-11-12 | Compositions and methods for recombinational cloning of nucleic acid molecules |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1131078A1 EP1131078A1 (en) | 2001-09-12 |
EP1131078A4 true EP1131078A4 (en) | 2005-08-24 |
Family
ID=22321549
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99960316A Withdrawn EP1131078A4 (en) | 1998-11-13 | 1999-11-12 | Compositions and methods for recombinational cloning of nucleic acid molecules |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1131078A4 (en) |
AU (1) | AU1721600A (en) |
WO (1) | WO2000029000A1 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL135776A0 (en) | 1997-10-24 | 2001-05-20 | Life Technologies Inc | Recombinational cloning using nucleic acids having recombination sites |
AU774643B2 (en) * | 1999-03-02 | 2004-07-01 | Invitrogen Corporation | Compositions and methods for use in recombinational cloning of nucleic acids |
US6551828B1 (en) | 2000-06-28 | 2003-04-22 | Protemation, Inc. | Compositions and methods for generating expression vectors through site-specific recombination |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
WO2002053576A1 (en) | 2001-01-05 | 2002-07-11 | The General Hospital Corporation | Viral delivery system for infectious transfer of large genomic dna inserts |
EP1390394A4 (en) * | 2001-04-19 | 2004-05-26 | Invitrogen Corp | Compositions and methods for recombinational cloning of nucleic acid molecules |
JP2004532636A (en) * | 2001-05-21 | 2004-10-28 | インヴィトロジェン コーポレーション | Compositions and methods for use in isolating nucleic acid molecules |
CN1263860C (en) * | 2002-09-30 | 2006-07-12 | 华南农业大学 | Construction method of multigene carrier and its application |
DE602004027538D1 (en) | 2003-12-01 | 2010-07-15 | Life Technologies Corp | NUCLEIC ACID MOLECULES AND RECOMBINATION AGENTS CONTAINED THEREOF |
EP1997898A1 (en) * | 2007-06-01 | 2008-12-03 | Rijksuniversiteit Groningen | Means and methods for cloning nucleic acid sequences |
FR3028527A1 (en) | 2014-11-13 | 2016-05-20 | Pivert | IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5888732A (en) * | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
-
1999
- 1999-11-12 AU AU17216/00A patent/AU1721600A/en not_active Abandoned
- 1999-11-12 WO PCT/US1999/026871 patent/WO2000029000A1/en not_active Application Discontinuation
- 1999-11-12 EP EP99960316A patent/EP1131078A4/en not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5888732A (en) * | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
Non-Patent Citations (6)
Title |
---|
BUBECK P ET AL: "RAPID CLONING BY HOMOLOGOUS RECOMBINATION IN VIVO", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 15, 1993, pages 3601 - 3602, XP002064300, ISSN: 0305-1048 * |
HARTLEY JAMES L ET AL: "DNA cloning using in vitro site-specific recombination", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 10, no. 11, November 2000 (2000-11-01), pages 1788 - 1795, XP002187669, ISSN: 1088-9051 * |
LIU QINGHUA ET AL: "The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 8, no. 24, 3 December 1998 (1998-12-03), pages 1300 - 1309, XP002155212, ISSN: 0960-9822 * |
See also references of WO0029000A1 * |
SHASHIKANT C S L ET AL: "Recombinogenic targeting: a new approach to genomic analysis-a review", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 223, no. 1-2, 26 November 1998 (1998-11-26), pages 9 - 20, XP004153573, ISSN: 0378-1119 * |
ZHANG Y ET AL: "A new logic for DNA engineering using recombination in Escherichia coli", NATURE GENETICS, NEW YORK, NY, US, vol. 20, no. 2, October 1998 (1998-10-01), pages 123 - 128, XP002103371, ISSN: 1061-4036 * |
Also Published As
Publication number | Publication date |
---|---|
AU1721600A (en) | 2000-06-05 |
EP1131078A1 (en) | 2001-09-12 |
WO2000029000A9 (en) | 2000-11-09 |
WO2000029000A1 (en) | 2000-05-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20010612 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20050708 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20060311 |