EP1131078A4 - Compositions and methods for recombinational cloning of nucleic acid molecules - Google Patents

Compositions and methods for recombinational cloning of nucleic acid molecules

Info

Publication number
EP1131078A4
EP1131078A4 EP99960316A EP99960316A EP1131078A4 EP 1131078 A4 EP1131078 A4 EP 1131078A4 EP 99960316 A EP99960316 A EP 99960316A EP 99960316 A EP99960316 A EP 99960316A EP 1131078 A4 EP1131078 A4 EP 1131078A4
Authority
EP
European Patent Office
Prior art keywords
compositions
methods
nucleic acid
acid molecules
recombinational cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99960316A
Other languages
German (de)
French (fr)
Other versions
EP1131078A1 (en
Inventor
Gary F Gerard
Elizabeth Flynn
A-Li W Hu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of EP1131078A1 publication Critical patent/EP1131078A1/en
Publication of EP1131078A4 publication Critical patent/EP1131078A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP99960316A 1998-11-13 1999-11-12 Compositions and methods for recombinational cloning of nucleic acid molecules Withdrawn EP1131078A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10832498P 1998-11-13 1998-11-13
US108324P 1998-11-13
PCT/US1999/026871 WO2000029000A1 (en) 1998-11-13 1999-11-12 Compositions and methods for recombinational cloning of nucleic acid molecules

Publications (2)

Publication Number Publication Date
EP1131078A1 EP1131078A1 (en) 2001-09-12
EP1131078A4 true EP1131078A4 (en) 2005-08-24

Family

ID=22321549

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99960316A Withdrawn EP1131078A4 (en) 1998-11-13 1999-11-12 Compositions and methods for recombinational cloning of nucleic acid molecules

Country Status (3)

Country Link
EP (1) EP1131078A4 (en)
AU (1) AU1721600A (en)
WO (1) WO2000029000A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7670823B1 (en) * 1999-03-02 2010-03-02 Life Technologies Corp. Compositions for use in recombinational cloning of nucleic acids
NZ520579A (en) 1997-10-24 2004-08-27 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites and methods for synthesizing double stranded nucleic acids
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7214515B2 (en) 2001-01-05 2007-05-08 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic DNA inserts
US20030077804A1 (en) * 2001-04-19 2003-04-24 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
EP1451344A4 (en) * 2001-05-21 2005-03-23 Invitrogen Corp Compositions and methods for use in isolation of nucleic acid molecules
CN1263860C (en) * 2002-09-30 2006-07-12 华南农业大学 Construction method of multigene carrier and its application
DE602004027538D1 (en) 2003-12-01 2010-07-15 Life Technologies Corp NUCLEIC ACID MOLECULES AND RECOMBINATION AGENTS CONTAINED THEREOF
EP1997898A1 (en) * 2007-06-01 2008-12-03 Rijksuniversiteit Groningen Means and methods for cloning nucleic acid sequences
FR3028527A1 (en) 2014-11-13 2016-05-20 Pivert IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888732A (en) * 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888732A (en) * 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BUBECK P ET AL: "RAPID CLONING BY HOMOLOGOUS RECOMBINATION IN VIVO", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 15, 1993, pages 3601 - 3602, XP002064300, ISSN: 0305-1048 *
HARTLEY JAMES L ET AL: "DNA cloning using in vitro site-specific recombination", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 10, no. 11, November 2000 (2000-11-01), pages 1788 - 1795, XP002187669, ISSN: 1088-9051 *
LIU QINGHUA ET AL: "The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 8, no. 24, 3 December 1998 (1998-12-03), pages 1300 - 1309, XP002155212, ISSN: 0960-9822 *
See also references of WO0029000A1 *
SHASHIKANT C S L ET AL: "Recombinogenic targeting: a new approach to genomic analysis-a review", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 223, no. 1-2, 26 November 1998 (1998-11-26), pages 9 - 20, XP004153573, ISSN: 0378-1119 *
ZHANG Y ET AL: "A new logic for DNA engineering using recombination in Escherichia coli", NATURE GENETICS, NEW YORK, NY, US, vol. 20, no. 2, October 1998 (1998-10-01), pages 123 - 128, XP002103371, ISSN: 1061-4036 *

Also Published As

Publication number Publication date
AU1721600A (en) 2000-06-05
WO2000029000A1 (en) 2000-05-25
WO2000029000A9 (en) 2000-11-09
EP1131078A1 (en) 2001-09-12

Similar Documents

Publication Publication Date Title
EP1390394A4 (en) Compositions and methods for recombinational cloning of nucleic acid molecules
AU8909998A (en) Methods and compositions for cloning nucleic acid molecules
EP1062360A4 (en) Compositions and methods for enhanced synthesis of nucleic acid molecules
HUP9901321A3 (en) Methods and compositions for determining the sequence of nucleic acid molecules
IL130913A0 (en) Methods and compositions for detectin or quantification of nucleic acid species
AU3199699A (en) Modified nucleotides and methods useful for nucleic acid sequencing
AU2002365811A1 (en) Methods for the selection and cloning of nucleic acid molecules free of unwanted nucleotide sequence alterations
AU4065397A (en) Stable compositions for nucleic acid amplification and sequencing
PL347125A1 (en) Methods of nucleic acid amplification and sequencing
EP1141234A4 (en) Methods and compositions for isolation of nucleic acid molecules
EP1451344A4 (en) Compositions and methods for use in isolation of nucleic acid molecules
EP1339875A4 (en) Compositions and methods for rapidly generating recombinant nucleic acid molecules
EP1165586A4 (en) TCL-1b GENE AND PROTEIN AND RELATED METHODS AND COMPOSITIONS
EP1224304A4 (en) Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
EP1131078A4 (en) Compositions and methods for recombinational cloning of nucleic acid molecules
AU2001285219A1 (en) Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
EP1144682A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
AU6950096A (en) Methods and compositions for nucleic acid targeting
AU8505898A (en) Nucleic acid compositions and methods of introducing nucleic acids into cells
EP1080225A4 (en) Compositions and methods for the pulmonary delivery of nucleic acids
EP0981535A4 (en) Methods for production and purification of nucleic acid molecules
AU6795201A (en) Nucleic acid sequences and methods for the modification of plant gene expression
AU2180000A (en) Nucleic acid molecules of shrimp
AU6172200A (en) Nucleic acid constructs for the modification of polyamine levels in plants
EP1163340A4 (en) Compositions and methods for the modification of gene expression

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20010612

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

A4 Supplementary search report drawn up and despatched

Effective date: 20050708

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20060311