EP1131078A4 - Compositions et methodes de clonage recombinatoire de molecules d'acide nucleique - Google Patents

Compositions et methodes de clonage recombinatoire de molecules d'acide nucleique

Info

Publication number
EP1131078A4
EP1131078A4 EP99960316A EP99960316A EP1131078A4 EP 1131078 A4 EP1131078 A4 EP 1131078A4 EP 99960316 A EP99960316 A EP 99960316A EP 99960316 A EP99960316 A EP 99960316A EP 1131078 A4 EP1131078 A4 EP 1131078A4
Authority
EP
European Patent Office
Prior art keywords
compositions
methods
nucleic acid
acid molecules
recombinational cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99960316A
Other languages
German (de)
English (en)
Other versions
EP1131078A1 (fr
Inventor
Gary F Gerard
Elizabeth Flynn
A-Li W Hu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of EP1131078A1 publication Critical patent/EP1131078A1/fr
Publication of EP1131078A4 publication Critical patent/EP1131078A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP99960316A 1998-11-13 1999-11-12 Compositions et methodes de clonage recombinatoire de molecules d'acide nucleique Withdrawn EP1131078A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10832498P 1998-11-13 1998-11-13
US108324P 1998-11-13
PCT/US1999/026871 WO2000029000A1 (fr) 1998-11-13 1999-11-12 Compositions et methodes de clonage recombinatoire de molecules d'acide nucleique

Publications (2)

Publication Number Publication Date
EP1131078A1 EP1131078A1 (fr) 2001-09-12
EP1131078A4 true EP1131078A4 (fr) 2005-08-24

Family

ID=22321549

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99960316A Withdrawn EP1131078A4 (fr) 1998-11-13 1999-11-12 Compositions et methodes de clonage recombinatoire de molecules d'acide nucleique

Country Status (3)

Country Link
EP (1) EP1131078A4 (fr)
AU (1) AU1721600A (fr)
WO (1) WO2000029000A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
IL135776A0 (en) 1997-10-24 2001-05-20 Life Technologies Inc Recombinational cloning using nucleic acids having recombination sites
DE60042969D1 (de) * 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002053576A1 (fr) 2001-01-05 2002-07-11 The General Hospital Corporation Système viral de livraison pour le transfert infectieux de fragments d'insertion longs d'adn génomique
CA2444195A1 (fr) * 2001-04-19 2002-10-31 Invitrogen Corporation Compositions et procedes destines au clonage recombinant de molecules d'acides nucleiques
CN1263860C (zh) * 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
WO2005054438A2 (fr) 2003-12-01 2005-06-16 Invitrogen Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
EP1997898A1 (fr) * 2007-06-01 2008-12-03 Rijksuniversiteit Groningen Outils et procédé de clonage de séquences d'acides nucléiques
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888732A (en) * 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888732A (en) * 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BUBECK P ET AL: "RAPID CLONING BY HOMOLOGOUS RECOMBINATION IN VIVO", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 15, 1993, pages 3601 - 3602, XP002064300, ISSN: 0305-1048 *
HARTLEY JAMES L ET AL: "DNA cloning using in vitro site-specific recombination", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 10, no. 11, November 2000 (2000-11-01), pages 1788 - 1795, XP002187669, ISSN: 1088-9051 *
LIU QINGHUA ET AL: "The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 8, no. 24, 3 December 1998 (1998-12-03), pages 1300 - 1309, XP002155212, ISSN: 0960-9822 *
See also references of WO0029000A1 *
SHASHIKANT C S L ET AL: "Recombinogenic targeting: a new approach to genomic analysis-a review", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 223, no. 1-2, 26 November 1998 (1998-11-26), pages 9 - 20, XP004153573, ISSN: 0378-1119 *
ZHANG Y ET AL: "A new logic for DNA engineering using recombination in Escherichia coli", NATURE GENETICS, NEW YORK, NY, US, vol. 20, no. 2, October 1998 (1998-10-01), pages 123 - 128, XP002103371, ISSN: 1061-4036 *

Also Published As

Publication number Publication date
AU1721600A (en) 2000-06-05
WO2000029000A9 (fr) 2000-11-09
WO2000029000A1 (fr) 2000-05-25
EP1131078A1 (fr) 2001-09-12

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