EP1130399A3 - Verfahren und Produkt zur Bestimmung der Folge von Peptiden unter Verwendung eines Massenspektrometers - Google Patents

Verfahren und Produkt zur Bestimmung der Folge von Peptiden unter Verwendung eines Massenspektrometers Download PDF

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Publication number
EP1130399A3
EP1130399A3 EP01112292A EP01112292A EP1130399A3 EP 1130399 A3 EP1130399 A3 EP 1130399A3 EP 01112292 A EP01112292 A EP 01112292A EP 01112292 A EP01112292 A EP 01112292A EP 1130399 A3 EP1130399 A3 EP 1130399A3
Authority
EP
European Patent Office
Prior art keywords
peptides
product
mass spectrometer
sequence determination
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP01112292A
Other languages
English (en)
French (fr)
Other versions
EP1130399A2 (de
EP1130399B1 (de
Inventor
Brian T. Chait
Ronald Beavis
Rong Wang
Stephen B.H. Kent
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rockefeller University
Original Assignee
Rockefeller University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rockefeller University filed Critical Rockefeller University
Publication of EP1130399A2 publication Critical patent/EP1130399A2/de
Publication of EP1130399A3 publication Critical patent/EP1130399A3/de
Application granted granted Critical
Publication of EP1130399B1 publication Critical patent/EP1130399B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP01112292A 1992-05-29 1993-05-27 Verfahren zur Bestimmung der Folge von Peptiden unter Verwendung eines Massenspektrometers Expired - Lifetime EP1130399B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US89117792A 1992-05-29 1992-05-29
US891177 1992-05-29
EP93914216A EP0664884A4 (de) 1992-05-29 1993-05-27 Methode und produkt für die bestimmung der folge von peptiden unter verwendung eines massenspektrometers.

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP93914216A Division EP0664884A4 (de) 1992-05-29 1993-05-27 Methode und produkt für die bestimmung der folge von peptiden unter verwendung eines massenspektrometers.
EP93914216.2 Division 1993-12-09

Publications (3)

Publication Number Publication Date
EP1130399A2 EP1130399A2 (de) 2001-09-05
EP1130399A3 true EP1130399A3 (de) 2002-09-11
EP1130399B1 EP1130399B1 (de) 2009-04-01

Family

ID=25397744

Family Applications (2)

Application Number Title Priority Date Filing Date
EP01112292A Expired - Lifetime EP1130399B1 (de) 1992-05-29 1993-05-27 Verfahren zur Bestimmung der Folge von Peptiden unter Verwendung eines Massenspektrometers
EP93914216A Withdrawn EP0664884A4 (de) 1992-05-29 1993-05-27 Methode und produkt für die bestimmung der folge von peptiden unter verwendung eines massenspektrometers.

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP93914216A Withdrawn EP0664884A4 (de) 1992-05-29 1993-05-27 Methode und produkt für die bestimmung der folge von peptiden unter verwendung eines massenspektrometers.

Country Status (8)

Country Link
US (4) US6271037B1 (de)
EP (2) EP1130399B1 (de)
JP (1) JPH07507394A (de)
AT (1) ATE427493T1 (de)
AU (1) AU685055B2 (de)
CA (1) CA2136717A1 (de)
DE (1) DE69334274D1 (de)
WO (1) WO1993024834A1 (de)

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Title
AEBERSOLD R ET AL: "DESIGN, SYNTHESIS, AND CHARACTERIZATION OF A PROTEIN SEQUENCING REAGENT YIELDING AMINO ACID DERIVATIVES WITH ENHANCED DETECTABILITY BY MASS SPECTROMETRY", PROTEIN SCIENCE, CAMBRIDGE UNIVERSITY PRESS, CAMBRIDGE, GB, vol. 1, 1992, pages 494 - 503, XP000998678, ISSN: 0961-8368 *
BEAVIS R C ET AL: "FACTORS AFFECTING THE ULTRAVIOLET LASER DESORPTION OF PROTEINS", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, HEYDEN, LONDON, GB, vol. 3, no. 7, 1989, pages 233 - 237, XP001009467, ISSN: 0951-4198 *
SELF R ET AL: "THE COMBINED USE OF ENZYMATIC HYDROLYSIS AND FAST ATOM BOMBARDMENT MASS SPECTROMETRY FOR PEPTIDE SEQUENCING", BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, WILEY, LONDON, GB, vol. 10, no. 2, 1983, pages 78 - 82, XP001009599, ISSN: 0887-6134 *

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WO1993024834A1 (en) 1993-12-09
US20050153454A1 (en) 2005-07-14
JPH07507394A (ja) 1995-08-10
CA2136717A1 (en) 1993-12-09
US7341874B2 (en) 2008-03-11
AU685055B2 (en) 1998-01-15
US6271037B1 (en) 2001-08-07
EP1130399A2 (de) 2001-09-05
US20030059952A1 (en) 2003-03-27
DE69334274D1 (de) 2009-05-14
EP0664884A4 (de) 1997-05-02
ATE427493T1 (de) 2009-04-15
EP0664884A1 (de) 1995-08-02
US20040265944A1 (en) 2004-12-30
US6750061B2 (en) 2004-06-15
EP1130399B1 (de) 2009-04-01
AU4395893A (en) 1993-12-30

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