CA2406356A1 - Method and product for the sequence determination of peptides using a mass spectrometer - Google Patents

Method and product for the sequence determination of peptides using a mass spectrometer

Info

Publication number
CA2406356A1
CA2406356A1 CA002406356A CA2406356A CA2406356A1 CA 2406356 A1 CA2406356 A1 CA 2406356A1 CA 002406356 A CA002406356 A CA 002406356A CA 2406356 A CA2406356 A CA 2406356A CA 2406356 A1 CA2406356 A1 CA 2406356A1
Authority
CA
Canada
Prior art keywords
peptides
product
mass spectrometer
sequence determination
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002406356A
Other languages
French (fr)
Other versions
CA2406356C (en
Inventor
Brian T. Chait
Ronald Beavis
Rong Wang
Stephen B. H. Kent
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rockefeller University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from CA002136717A external-priority patent/CA2136717A1/en
Publication of CA2406356A1 publication Critical patent/CA2406356A1/en
Application granted granted Critical
Publication of CA2406356C publication Critical patent/CA2406356C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Method is described for sequencing polypeptides by forming peptide ladders comprising a series of polypeptides in which adjacent members of the series vary by one amino acid residue and determinin g the identity and position of each amino acid in the polypeptide by mass spectroscopy.
CA002406356A 1992-05-29 1993-05-27 Method and product for the sequence determination of peptides using a mass spectrometer Expired - Lifetime CA2406356C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US89117792A 1992-05-29 1992-05-29
US07/891,177 1992-05-29
CA002136717A CA2136717A1 (en) 1992-05-29 1993-05-27 Method and product for the sequence determination of peptides using a mass spectrometer

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002136717A Division CA2136717A1 (en) 1992-05-29 1993-05-27 Method and product for the sequence determination of peptides using a mass spectrometer

Publications (2)

Publication Number Publication Date
CA2406356A1 true CA2406356A1 (en) 1993-12-09
CA2406356C CA2406356C (en) 2007-01-30

Family

ID=25677638

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002406356A Expired - Lifetime CA2406356C (en) 1992-05-29 1993-05-27 Method and product for the sequence determination of peptides using a mass spectrometer

Country Status (1)

Country Link
CA (1) CA2406356C (en)

Also Published As

Publication number Publication date
CA2406356C (en) 2007-01-30

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Effective date: 20130527