EP1092147A1 - Regulation du flux et des matieres destinee a des micro-dispositifs - Google Patents
Regulation du flux et des matieres destinee a des micro-dispositifsInfo
- Publication number
- EP1092147A1 EP1092147A1 EP99927515A EP99927515A EP1092147A1 EP 1092147 A1 EP1092147 A1 EP 1092147A1 EP 99927515 A EP99927515 A EP 99927515A EP 99927515 A EP99927515 A EP 99927515A EP 1092147 A1 EP1092147 A1 EP 1092147A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- fluid pathway
- constrained
- sample
- fluid
- channel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000000463 material Substances 0.000 title abstract description 25
- 239000012530 fluid Substances 0.000 claims abstract description 81
- 238000000034 method Methods 0.000 claims abstract description 34
- 238000013508 migration Methods 0.000 claims abstract description 26
- 230000005012 migration Effects 0.000 claims abstract description 26
- 230000037361 pathway Effects 0.000 claims description 44
- 238000002347 injection Methods 0.000 claims description 17
- 239000007924 injection Substances 0.000 claims description 17
- 238000000926 separation method Methods 0.000 claims description 17
- 239000000872 buffer Substances 0.000 claims description 15
- 238000005370 electroosmosis Methods 0.000 claims description 10
- 230000001276 controlling effect Effects 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 2
- 238000004094 preconcentration Methods 0.000 abstract description 14
- 230000007717 exclusion Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 76
- 239000004005 microsphere Substances 0.000 description 18
- 238000002474 experimental method Methods 0.000 description 11
- 239000000126 substance Substances 0.000 description 9
- 239000004816 latex Substances 0.000 description 8
- 229920000126 latex Polymers 0.000 description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 7
- 238000001179 sorption measurement Methods 0.000 description 6
- 238000005515 capillary zone electrophoresis Methods 0.000 description 5
- 230000005684 electric field Effects 0.000 description 5
- 239000005350 fused silica glass Substances 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000005251 capillar electrophoresis Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000002801 charged material Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000005086 pumping Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 150000007942 carboxylates Chemical class 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000012468 concentrated sample Substances 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000012305 analytical separation technique Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000005686 electrostatic field Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
Definitions
- the present invention provides an analytical device, either microchip- or capillary-based, having the means to exclude specific sample components of interest from a capillary or channel for the purpose of preconcentration or control of movement of sample components.
- a control system includes a means for controlling the flow of the fluid in the channel and the placement of an electrode at the immediate entrance of each channel on such devices so that material may be directly manipulated by either or both of the effects of both bulk flow and electrically driven migration.
- Capillary zone electrophoresis is an efficient analytical separation technique which utilizes differences in mobility of sample components in an electric field based on the electrical charge and molecular site and shape of the sample component.
- Conventional CZE systems typically comprise a buffer- filled capillary with outlet and inlet ends disposed in two reservoirs into which one sample is injected, a means for applying voltage to the capillary resulting in migration of the sample through the capillary, and a means for detecting the sample zone.
- Figure 5 is a graph showing the normalized fluorescence intensity versus distance outside the capillary entrance for two control experiments
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Cette invention concerne d'une manière générale, des procédés et des dispositifs qui permettent de réguler le mouvement des fluides et des constituants d'échantillons chargés électriquement présents dans ces fluides. De manière plus particulière, l'invention permet d'exclure ou de concentrer des constituants d'échantillons spécifiquement choisis qui se trouvent dans un fluide. L'invention porte sur un dispositif d'analyse, comprenant des micropuces ou des capillaires, et qui est doté d'un système conçu pour exclure d'un capillaire ou d'un canal des constituants d'échantillons spécifiques recherchés en vue de préconcentrer ou de réguler le mouvement des constituants d'échantillons. Un tel système de régulation est doté d'un dispositif qui régule l'écoulement du fluide dans le canal et le positionnement d'une électrode au niveau de l'entrée immédiate de chaque canal sur de tels dispositifs, pour que la matière puisse être directement manipulée par les effets du volume de l'écoulement et la migration induite par les électrons.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8895698P | 1998-06-11 | 1998-06-11 | |
| US88956P | 1998-06-11 | ||
| PCT/US1999/013340 WO1999064851A1 (fr) | 1998-06-11 | 1999-06-11 | Regulation du flux et des matieres destinee a des micro-dispositifs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1092147A1 true EP1092147A1 (fr) | 2001-04-18 |
Family
ID=22214492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP99927515A Withdrawn EP1092147A1 (fr) | 1998-06-11 | 1999-06-11 | Regulation du flux et des matieres destinee a des micro-dispositifs |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1092147A1 (fr) |
| JP (1) | JP2002517751A (fr) |
| CA (1) | CA2328400A1 (fr) |
| WO (1) | WO1999064851A1 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2477287A (en) * | 2010-01-27 | 2011-08-03 | Izon Science Ltd | Control of particle flow in an aperture |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7695689B2 (en) | 2002-07-18 | 2010-04-13 | National Institute Of Advanced Industrial Science And Technology | Micro reactor device and method of manufacturing micro reactor device |
| US11090660B2 (en) | 2016-08-10 | 2021-08-17 | Arizona Board Of Regents On Behalf Of Arizona State University | Hyper efficient separations device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5453382A (en) * | 1991-08-05 | 1995-09-26 | Indiana University Foundation | Electrochromatographic preconcentration method |
| US5200050A (en) * | 1991-10-25 | 1993-04-06 | Washington State University Research Foundation | Electrophoretic processor and methods |
| US6001229A (en) * | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| US6093327A (en) * | 1995-03-03 | 2000-07-25 | Anderson, Jr.; James M. | Apparatuses and methods for electrochemically modifying the retention of species on chromatography material |
-
1999
- 1999-06-11 JP JP2000553795A patent/JP2002517751A/ja active Pending
- 1999-06-11 EP EP99927515A patent/EP1092147A1/fr not_active Withdrawn
- 1999-06-11 WO PCT/US1999/013340 patent/WO1999064851A1/fr not_active Application Discontinuation
- 1999-06-11 CA CA002328400A patent/CA2328400A1/fr not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO9964851A1 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2477287A (en) * | 2010-01-27 | 2011-08-03 | Izon Science Ltd | Control of particle flow in an aperture |
| GB2477287B (en) * | 2010-01-27 | 2012-02-15 | Izon Science Ltd | Control of particle flow in an aperture |
| US9052262B2 (en) | 2010-01-27 | 2015-06-09 | Izon Science Limited | Control of particle flow in an aperture |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999064851A1 (fr) | 1999-12-16 |
| JP2002517751A (ja) | 2002-06-18 |
| CA2328400A1 (fr) | 1999-12-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5858187A (en) | Apparatus and method for performing electrodynamic focusing on a microchip | |
| US8062903B2 (en) | Droplet compartmentalization for chemical separation and on-line sampling | |
| EP0727661B1 (fr) | Procédé pour mélanger des liquides par électro-osmose | |
| Lee et al. | Direct control of the electroosmosis in capillary zone electrophoresis by using an external electric field | |
| Hashimoto et al. | Microchip capillary electrophoresis using on-line chemiluminescence detection | |
| JP3656165B2 (ja) | ミクロカラム分離技術における試料導入を制御するための方法及びその方法を使用するサンプリング装置 | |
| US5942093A (en) | Electro-osmotically driven liquid delivery method and apparatus | |
| US5429728A (en) | Electroosmotic flow control using back pressure in capillary electrophoresis | |
| US6890411B1 (en) | Control of flow and materials for micro devices | |
| US5141621A (en) | Capillary electrophoresis injection device and method | |
| JP3011914B2 (ja) | マイクロカラムによる動電分離用のオンカラム式電導度検出器 | |
| EP0401033A2 (fr) | Dispositif d'électrophorèse capillaire utilisant une structure pour réaliser un contact électrique par moyen de la mobilité des ions | |
| JP2007108181A (ja) | 電子ピペッタおよび電気泳動バイアスのための補償手段 | |
| US5378334A (en) | System for measuring and controlling electroosmosis in separation techniques | |
| Wang et al. | Measurement of electroosmotic flow in capillary and microchip electrophoresis | |
| Xu | Tutorial: capillary electrophoresis | |
| US20020130044A1 (en) | Mechanical control of fluids in micro-analytical devices | |
| JPH1010088A (ja) | キャピラリ−電気泳動装置 | |
| Opekar et al. | Dual-channel capillary electrophoresis for simultaneous determination of cations and anions | |
| Lee et al. | Analysis of separation efficiency in capillary electrophoresis with direct control of electroosmosis by using an external electric field | |
| JP2002529235A (ja) | 極微量流を制御するための実用装置 | |
| WO1999064851A1 (fr) | Regulation du flux et des matieres destinee a des micro-dispositifs | |
| Xia et al. | On‐chip pressure generation using a gel membrane fabricated outside of the microfluidic network | |
| EP0304295A1 (fr) | Méthode d'analyse électrocinétique et appareil à dissipation de chaleur | |
| Opekar et al. | Characterization of various geometric arrangements of “air‐assisted” flow gating interfaces for capillary electrophoresis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 20001027 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
| 18D | Application deemed to be withdrawn |
Effective date: 20060103 |