EP1053353A1 - Elutionsreagentien, verfahren und reagentiensätze zur isolierung von dns - Google Patents

Elutionsreagentien, verfahren und reagentiensätze zur isolierung von dns

Info

Publication number
EP1053353A1
EP1053353A1 EP99905618A EP99905618A EP1053353A1 EP 1053353 A1 EP1053353 A1 EP 1053353A1 EP 99905618 A EP99905618 A EP 99905618A EP 99905618 A EP99905618 A EP 99905618A EP 1053353 A1 EP1053353 A1 EP 1053353A1
Authority
EP
European Patent Office
Prior art keywords
dna
reagent
solid support
buffer
eluting reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99905618A
Other languages
English (en)
French (fr)
Inventor
Ellen M. Heath
Ruth M. Shuman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen North American Holdings Inc
Original Assignee
Gentra Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gentra Systems Inc filed Critical Gentra Systems Inc
Publication of EP1053353A1 publication Critical patent/EP1053353A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
EP99905618A 1998-02-02 1999-02-02 Elutionsreagentien, verfahren und reagentiensätze zur isolierung von dns Withdrawn EP1053353A1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12642998P 1998-02-02 1998-02-02
US12641398P 1998-02-02 1998-02-02
US126429P 1998-02-02
US126413P 1998-02-02
PCT/US1999/002190 WO1999039010A1 (en) 1998-02-02 1999-02-02 Eluting reagents, methods and kits for isolating dna

Publications (1)

Publication Number Publication Date
EP1053353A1 true EP1053353A1 (de) 2000-11-22

Family

ID=26824623

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99905618A Withdrawn EP1053353A1 (de) 1998-02-02 1999-02-02 Elutionsreagentien, verfahren und reagentiensätze zur isolierung von dns

Country Status (5)

Country Link
EP (1) EP1053353A1 (de)
JP (2) JP2004500002A (de)
AU (1) AU2574299A (de)
CA (1) CA2319691C (de)
WO (1) WO1999039010A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1027145A4 (de) 1997-09-17 2004-08-25 Gentra Systems Inc Vorrichtung und verfahren zur isolierung von nukleinsäure
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
WO2000040697A1 (en) * 1999-01-06 2000-07-13 Invitrogen Corporation Methods and compositions for isolation of nucleic acid molecules
JP2004518944A (ja) 2000-07-18 2004-06-24 インヴィトロジェン コーポレーション ゲル材料を細分および濾過して分子を抽出するための装置および方法
CA2431644A1 (en) 2000-12-12 2002-06-20 Invitrogen Corporation Compositions and methods for the release of nucleic acid molecules from solid matrices
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
WO2003057910A2 (en) 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
JP5441290B2 (ja) * 2002-10-04 2014-03-12 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸精製システムの媒体上に核酸を保存するツールとして、化学物質を用いる方法および材料
JP2008220380A (ja) * 2003-10-31 2008-09-25 Fujifilm Corp 核酸の分離精製方法
CA2586532C (en) * 2004-11-05 2014-03-25 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
US9243289B2 (en) * 2013-12-23 2016-01-26 Roche Molecular Systems, Inc. Method for screening reagents used in PCR assays
CN107002077A (zh) * 2014-11-07 2017-08-01 约翰霍普金斯大学 用于从血浆纯化核酸的无离液剂和无挥发物的方法
AU2016262545B2 (en) * 2015-05-12 2022-06-16 Gen-Probe Incorporated Method of pooling blood samples
CA3055981A1 (en) 2017-03-15 2018-09-20 Ancestry.Com Dna, Llc Sample collection device and method

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
JPH04158784A (ja) * 1990-10-24 1992-06-01 Tax Adm Agency 酵母細胞壁溶解酵素の製造法及びその利用
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
JPH06277040A (ja) * 1991-02-13 1994-10-04 Tax Adm Agency 酵母細胞壁溶解酵素の製造法及び溶解法
JP3294288B2 (ja) * 1991-02-22 2002-06-24 明治乳業株式会社 乳酸桿菌由来の新規なプラスミドｐＢＵＬ１ 及びその誘導体
MX9202037A (es) * 1991-05-03 1992-11-01 Becton Dickinson Co Purificacion por filtracion de adn.
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
EP0580305B1 (de) * 1992-07-02 1999-09-22 Edge Biosystems, Inc. Verfahren und Material für Reinigung von Nukleinsäuren
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
US5438127A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
WO1995021849A1 (de) * 1994-02-11 1995-08-17 Qiagen Gmbh Verfahren zur trennung von doppelstrang/einzelstrangnukleinsäurestrukturen
ATE184013T1 (de) * 1994-06-14 1999-09-15 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
DE69633239T2 (de) * 1995-06-07 2005-09-08 Whatman Plc, Maidstone Trockenes festes medium zur lagerung und analyse von genetischem material
US5702884A (en) * 1996-03-12 1997-12-30 Johnson & Johnson Clinical Diagnostics, Inc. Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis
US5777098A (en) * 1996-07-23 1998-07-07 University Of North Dakota Medical Education Research Foundation DNA purification procedure
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
WO1999039009A1 (en) * 1998-02-02 1999-08-05 Gentra Systems, Inc. Processes for isolating, amplifying and characterizing dna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9939010A1 *

Also Published As

Publication number Publication date
WO1999039010A1 (en) 1999-08-05
CA2319691A1 (en) 1999-08-05
CA2319691C (en) 2012-12-18
AU2574299A (en) 1999-08-16
JP2004500002A (ja) 2004-01-08
JP2012157366A (ja) 2012-08-23

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