EP1017833A1 - Blütenblatt-spezifischer promoter und verfahren zur gewinung von pflanzen mit blumen ohne blütenbläter - Google Patents

Blütenblatt-spezifischer promoter und verfahren zur gewinung von pflanzen mit blumen ohne blütenbläter

Info

Publication number
EP1017833A1
EP1017833A1 EP98945367A EP98945367A EP1017833A1 EP 1017833 A1 EP1017833 A1 EP 1017833A1 EP 98945367 A EP98945367 A EP 98945367A EP 98945367 A EP98945367 A EP 98945367A EP 1017833 A1 EP1017833 A1 EP 1017833A1
Authority
EP
European Patent Office
Prior art keywords
plants
sequence
petals
nucleotide
petal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98945367A
Other languages
English (en)
French (fr)
Inventor
Inès BROCARD
Florence Charlot
Evelyne Teoule
Philippe Guerche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Recherche Agronomique INRA
Original Assignee
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Recherche Agronomique INRA filed Critical Institut National de la Recherche Agronomique INRA
Publication of EP1017833A1 publication Critical patent/EP1017833A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8225—Leaf-specific, e.g. including petioles, stomata
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Definitions

  • the present invention relates in particular to a specific promoter for petals and to a process for obtaining flowering plants without petals.
  • the present invention therefore relates to a nucleotide sequence of which it has been demonstrated that the corresponding gene is expressed specifically in the petal, this nucleotide sequence corresponds to SEQ ID N ° 5
  • this region extends preferably from nucleotide 1 to nucleotide 3233 (corresponding to the downstream site) and even more preferably from nucleotide 2911 to nucleotide 3233 of SEQ ID N ° 5
  • FIG. 4 represents the partial restriction maps of the genomic clones (A Downstream, B BamHl, El EcoRl, EV EcoRW, HH ndlll, Hc H cll,? Psil, S Sacl, SI S ⁇ / l, Xb Xba ⁇ , ⁇ X Xho )
  • FIG. 7 illustrates the constructions carried out with the 322 bp promoter of the genomic clone 4 1 1 promoter of 322 bp of the genomic clone 4.1.1 coding sequence of the reporter gene gus coding sequence of the wild barnase gene coding sequence of the mutated barnase gene terminator of the nopaline synthase gene CaS 19S terminator
  • the first step consisted in obtaining complementary DNA clones (cDNA) expressed specifically in the petal.
  • cDNA complementary DNA clones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP98945367A 1997-09-23 1998-09-23 Blütenblatt-spezifischer promoter und verfahren zur gewinung von pflanzen mit blumen ohne blütenbläter Withdrawn EP1017833A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9711832A FR2768746B1 (fr) 1997-09-23 1997-09-23 Promoteur specifique des petales et procede d'obtention de plantes a fleurs sans petale
FR9711832 1997-09-23
PCT/FR1998/002043 WO1999015679A1 (fr) 1997-09-23 1998-09-23 Promoteur specifique des petales et procede d'obtention de plantes a fleurs sans petale

Publications (1)

Publication Number Publication Date
EP1017833A1 true EP1017833A1 (de) 2000-07-12

Family

ID=9511383

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98945367A Withdrawn EP1017833A1 (de) 1997-09-23 1998-09-23 Blütenblatt-spezifischer promoter und verfahren zur gewinung von pflanzen mit blumen ohne blütenbläter

Country Status (6)

Country Link
EP (1) EP1017833A1 (de)
JP (1) JP2001517450A (de)
AU (1) AU740911C (de)
CA (1) CA2304569A1 (de)
FR (1) FR2768746B1 (de)
WO (1) WO1999015679A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109362562A (zh) * 2018-12-26 2019-02-22 江西省农业科学院作物研究所 一种带有桔黄花色标记性状的甘蓝型油菜恢复系选育方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002223581A1 (en) * 2000-10-03 2002-04-15 Bayer Bioscience N.V. Brassicaceae with altered floral development
ATE455176T1 (de) * 2002-09-03 2010-01-15 Sungene Gmbh Transgene expressionskassetten zur expression von nukleinsäuren in nicht-reproduktiven blütengeweben von pflanzen
FR2844142B1 (fr) 2002-09-11 2007-08-17 Bayer Cropscience Sa Plantes transformees a biosynthese de prenylquinones amelioree
FR2848570B1 (fr) 2002-12-12 2005-04-01 Bayer Cropscience Sa Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant
JP2012005348A (ja) * 2008-10-10 2012-01-12 Nagoya Univ 花弁特異的プロモーター及びその利用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9116071D0 (en) * 1991-07-25 1991-09-11 Sandoz Ltd Improvements in or relating to organic compounds
AU671272B2 (en) * 1992-01-09 1996-08-22 John Innes Foundation Regulation of plant genes
WO1994000582A2 (en) * 1992-06-30 1994-01-06 Bruinsma Seeds B.V. A method for obtaining a plant having altered floral morphology and a method for protecting plants against pest insects
EP0823480A1 (de) * 1996-08-06 1998-02-11 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Kontrollierte Genexpression in Pflanzen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9915679A1 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109362562A (zh) * 2018-12-26 2019-02-22 江西省农业科学院作物研究所 一种带有桔黄花色标记性状的甘蓝型油菜恢复系选育方法

Also Published As

Publication number Publication date
CA2304569A1 (fr) 1999-04-01
WO1999015679A1 (fr) 1999-04-01
AU740911B2 (en) 2001-11-15
AU740911C (en) 2002-07-25
FR2768746A1 (fr) 1999-03-26
FR2768746B1 (fr) 2001-06-08
JP2001517450A (ja) 2001-10-09
AU9270898A (en) 1999-04-12

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