EP0983514A1 - Procede et kit d'essai pour la detection de l'heroine - Google Patents

Procede et kit d'essai pour la detection de l'heroine

Info

Publication number
EP0983514A1
EP0983514A1 EP98912691A EP98912691A EP0983514A1 EP 0983514 A1 EP0983514 A1 EP 0983514A1 EP 98912691 A EP98912691 A EP 98912691A EP 98912691 A EP98912691 A EP 98912691A EP 0983514 A1 EP0983514 A1 EP 0983514A1
Authority
EP
European Patent Office
Prior art keywords
color
heroin
indicator
street
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP98912691A
Other languages
German (de)
English (en)
Other versions
EP0983514B1 (fr
Inventor
Baruch Glattstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Identa Ltd
Original Assignee
Identa Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Identa Ltd filed Critical Identa Ltd
Publication of EP0983514A1 publication Critical patent/EP0983514A1/fr
Application granted granted Critical
Publication of EP0983514B1 publication Critical patent/EP0983514B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9486—Analgesics, e.g. opiates, aspirine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/946—CNS-stimulants, e.g. cocaine, amphetamines

Definitions

  • the present invention relates to a process and test kit for the identification of heroin street drug. More specifically, the present invention relates to a process and test kit identification of heroin street drug without false positive with other street drugs.
  • "Street drug” in this invention is defined as a drug in its free base form or as its mixture with its salt.
  • a test kit can help an officer to detect the presence of heroin or alternatively to determine that a tested sample definitely is not of said drug.
  • the quickest test known today for drug detection is a color test in which the response of the drug to a specific reagent makes it possible to assign the drug to one or more classes.
  • This said known process is carried out with a sulfonated aromatic pH indicator which produces a color reaction in the presence of trace amounts of organic free base.
  • the sulfonated aromatic pH indicator is selected from the group consisting of bromophenol blue, bromochlorophenol blue and bromocresol green.
  • the test can be applied in two ways:
  • the filter paper is impregnated with the indicator by dipping it into a solution containing the indicator.
  • the material suspected as heroin street drug is placed on the filter paper.
  • the known commercial kit contains only the pH sulphonated indicator absorbed on hydophobic filter paper and the tedious procedure for detecting salts containing organic base was abandoned.
  • the above mentioned U.S. patent has several disadvantages:
  • the heroin drug testing kit is based on the fact that street drug heroin has at least trace amounts of heroin in its free base form.
  • heroin is not the only street drug manufactured in clandestine laboratories that contains organic base.
  • the other illicit wide spread drugs, containing organic base are: PCP (Phencyclidine) ,
  • MDMA 3,4-Methylenedioxymethamphetamine
  • the commercial kits use this indicator as a general indicator to the presence of organic base in illicit drugs. It was found that the commercial kit (where the indicator is placed on hydrophobic filer paper) reacts surprisingly with some drugs even where the drugs are in their pure salt form such as: Methamphetamine hydrochloride and diphenhydramine hydrochloride.
  • the sulfonated pH indicators cannot detect organic base in a pure salt.
  • the sulfonated indicators react to the above mentioned street drugs in the same purple color.
  • this sulfonated pH indicator has led the inventor to the development of another specific reagent for the heroin street drug.
  • This reagent is intendent to be used as a general indicator for drugs containing organic free base manufactured in clandestine laboratories. It then also gives a distinct color to a second reagent, that screens out the amphetamine, MDA and other street drugs manufactured in clandestine laboratories, that do not give the same color reaction as heroin.
  • the present invention there provides a process for the detection of heroin street drug and a differentiation of heroin street drug from other substances that may react to a free base indicator.
  • the present invention also provides a test kit for heroin detection comprising of; a) a lipophilic pH indicator solution wherein the indicator is dissolved in a solvent immiscible with water and said indicator is characterized by having a distinctive color response to heroin, amphetamine and MDA street drugs containing organic base and by having another color response to the other street drugs manufactured in clandestine laboratories. b) a Marquis reagent wherein by using this reagent the heroin gives a purple color and amphetamine methamphetamine and MDA gives an orange color. PCP does not give a color reaction with the reagent.
  • organic free base in a suspected substance is an indication for an illicit drug manufactured in a clandestine laboratory.
  • An organic free base can be detected by an appropriate indicator.
  • Sulfonephthalein indicators are a good option, because they react well to a free organic base when the indicator is dissolved in an organic solvent. These include the following sulfonephthaleins : bromophenol blue, bromocresol green and bromocresol purple.
  • the water solubilizing sulfonic acid group that is present in the sulfonephthalein cause the indicator to react with trace amounts of water and to give the same response (color) with water and with heroin. Therefore, the sulfonephthaleins are unsuited for (use in this field) for these types of testing kits.
  • the preferred indicator used in the present invention is a lipophilic pH indicator such as Tetrabromophenolphthalein ethyl ester that has a very good solubility in organic solvents, gives sensitive color changes and is completely insoluble in water. It intentionally lacks the water solubilizing sulfonic acid group that is present in the sulphonephthaleins .
  • Tetrabromophenolphthalein ethyl ester is the analog to bromophenol blue.
  • the color reaction of the lipophilic indicator is based on two stages; A small amount of the indicator is dissolved in a solvent
  • a trace amount of the suspected heroin is placed in a test tube and a few drops of the indicator are added and the solution is observed for any color change. If an organic free base is present, there will be a change in the color of the solution (depending on the characteristics of the indicator and the basicity of the base) .
  • Methamphetamine 3,4-Methylenedioxyamphetamine (MDA)
  • MDMA 4-Methylenedioxymethamphetamine
  • MDEA 3,4-Methylenedioxyethylamphetamine
  • MDA 3,4-Methylenedioxy-N-ethylamphetamine
  • Heroin, amphetamine and MDA will give an orange to red color with the indicator solution and the other drugs will give a violet color reaction. Adding hexane will cause the orange color to turn back to yellow and the red color reverts back to an orange or yellow. On the other hand, all the other suspected drugs will give a violet color, and by the addition of hexane, there will not be any change in the solution color.
  • stage A If a change of color is not seen in stage A, a few drops of buffer 7 are added (if there is any salt of an organic base, the buffer 7 partitions the organic base of the salt to the organic layer), if there is no color change, the suspected substance does not contain a salt of organic base and there is no need to use the Marquis test.
  • TBP Tetrabromophenolphthalein ethyl ester
  • stage A If there is no color change in stage A, a few drops of aqueous buffer 7 solution are added. If there is a color change, the suspected substance contains a salt of organic base. If there is no color change, the substance does not contain a salt of organic base at all .

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Emergency Medicine (AREA)
  • Pain & Pain Management (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Cette invention concerne un procédé de détection de l'héroïne qui est vendue dans la rue et de différenciation de l'héroïne vendue dans la rue par rapport à d'autres substances qui peuvent réagir avec un indicateur de base libre. Cette invention concerne également un kit de test pour la détection de l'héroïne, qui comprend : a) une solution d'indicateur de pH lipophile, ledit indicateur se caractérisant par une réponse de couleur distinctive à l'héroïne, aux amphétamines et aux drogues du type MDA vendues dans la rue qui contiennent une base organique et présentent une réponse d'une autre couleur aux autres drogues vendues dans la rue; b) un réactif Marquis qui, en utilisation, donne une couleur violette à l'héroïne et une couleur orange aux amphétamines, aux méthamphétamines et à la MDA.
EP98912691A 1997-04-10 1998-04-02 Procede et kit d'essai pour la detection de l'heroine Expired - Lifetime EP0983514B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IL12064397 1997-04-10
IL12064397A IL120643A (en) 1997-04-10 1997-04-10 Process and test kit for heroin detection
PCT/IL1998/000162 WO1998045714A1 (fr) 1997-04-10 1998-04-02 Procede et kit d'essai pour la detection de l'heroine

Publications (2)

Publication Number Publication Date
EP0983514A1 true EP0983514A1 (fr) 2000-03-08
EP0983514B1 EP0983514B1 (fr) 2007-12-19

Family

ID=11070018

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98912691A Expired - Lifetime EP0983514B1 (fr) 1997-04-10 1998-04-02 Procede et kit d'essai pour la detection de l'heroine

Country Status (5)

Country Link
EP (1) EP0983514B1 (fr)
AU (1) AU6745298A (fr)
DE (1) DE69838872D1 (fr)
IL (1) IL120643A (fr)
WO (1) WO1998045714A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11977085B1 (en) 2023-09-05 2024-05-07 Elan Ehrlich Date rape drug detection device and method of using same

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19857196A1 (de) * 1998-12-11 2000-06-15 Merck Patent Gmbh Verfahren und Schnellreagenz zum Nachweis von Methylendioxyverbindungen der Amphetaminreihe
IL147185A0 (en) * 2001-12-19 2002-08-14 Lattstein Ora A method for the detection of compounds comprising methylenedioxyphenyl and a testing kit for the same
CN114981655A (zh) * 2019-10-21 2022-08-30 韦里特克美国有限公司 在蒸气烟装置表面上和在蒸气烟液体制剂中的尼古丁、大麻素和滥用药物的检测
CN114354570A (zh) * 2021-12-16 2022-04-15 安徽中科赛飞尔科技有限公司 一种吸毒人员指纹表面毒品分子动sers检测方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4806487A (en) * 1987-05-29 1989-02-21 Analytical Innovations, Inc. Basic drug detection method
JPH0827275B2 (ja) * 1993-11-18 1996-03-21 警察庁長官 あへんの検出方法及び検出キット

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9845714A1 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11977085B1 (en) 2023-09-05 2024-05-07 Elan Ehrlich Date rape drug detection device and method of using same

Also Published As

Publication number Publication date
EP0983514B1 (fr) 2007-12-19
IL120643A (en) 2000-11-21
WO1998045714A1 (fr) 1998-10-15
DE69838872D1 (de) 2008-01-31
IL120643A0 (en) 1997-08-14
AU6745298A (en) 1998-10-30

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