EP0805979A1 - Agencement pour un appareil d'electrophorese sur gel - Google Patents

Agencement pour un appareil d'electrophorese sur gel

Info

Publication number
EP0805979A1
EP0805979A1 EP95941309A EP95941309A EP0805979A1 EP 0805979 A1 EP0805979 A1 EP 0805979A1 EP 95941309 A EP95941309 A EP 95941309A EP 95941309 A EP95941309 A EP 95941309A EP 0805979 A1 EP0805979 A1 EP 0805979A1
Authority
EP
European Patent Office
Prior art keywords
plates
fluorophore
optical filter
gel
laser
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP95941309A
Other languages
German (de)
English (en)
Inventor
Bengt-Göran Andersson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Pharmacia Biotech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biotech AB filed Critical Pharmacia Biotech AB
Publication of EP0805979A1 publication Critical patent/EP0805979A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)

Abstract

Cet appareil d'électrophorèse sur gel comprend un laser, qui est destiné à émettre un faisceau laser d'une longueur d'onde prédéterminée, afin d'exciter au moins une substance (4) qui est marquée à l'aide d'un fluorophore et qui est amenée à migrer le long d'un gel (1), lequel est placé entre deux plaques transparentes (2, 3), un détecteur (5) qui est placé à proximité de l'une (3) des plaques, afin de détecter la fluorescence émise après excitation du fluorophore ainsi qu'un filtre optique (6) qui est placé entre cette plaque (3) et ledit détecteur et qui est destiné à bloquer la lumière de cette longueur d'onde prédéterminée, le filtre optique (6) étant conçu pour bloquer également la fluorescence intrinsèque desdites plaques (2, 3).
EP95941309A 1994-12-08 1995-12-08 Agencement pour un appareil d'electrophorese sur gel Withdrawn EP0805979A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9404274A SE9404274D0 (sv) 1994-12-08 1994-12-08 Anordning vid en gelelektroforesapparat
SE9404274 1994-12-08
PCT/SE1995/001482 WO1996018099A1 (fr) 1994-12-08 1995-12-08 Agencement pour un appareil d'electrophorese sur gel

Publications (1)

Publication Number Publication Date
EP0805979A1 true EP0805979A1 (fr) 1997-11-12

Family

ID=20396268

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95941309A Withdrawn EP0805979A1 (fr) 1994-12-08 1995-12-08 Agencement pour un appareil d'electrophorese sur gel

Country Status (3)

Country Link
EP (1) EP0805979A1 (fr)
SE (1) SE9404274D0 (fr)
WO (1) WO1996018099A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10132784A (ja) * 1996-11-05 1998-05-22 Hitachi Electron Eng Co Ltd Ｄｎａ塩基配列決定装置
GB9624927D0 (en) * 1996-11-29 1997-01-15 Oxford Glycosciences Uk Ltd Gels and their use
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
GB9811656D0 (en) 1998-05-29 1998-07-29 Oxford Glycosciences Uk Ltd Gels, methods and apparatus for identification and characterization of biomolecules
US10531655B2 (en) 2011-12-02 2020-01-14 The Regents Of The University Of California Reperfusion protection solution and uses thereof
US9295393B2 (en) 2012-11-09 2016-03-29 Elwha Llc Embolism deflector

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3618605A1 (de) * 1986-06-03 1987-12-10 Europ Lab Molekularbiolog Vorrichtung zur detektion von zur photonenemission anregbaren stoffen
JP2594925B2 (ja) * 1987-01-23 1997-03-26 株式会社日立製作所 電気泳動装置
US5062942A (en) * 1989-04-12 1991-11-05 Hitachi, Ltd. Fluorescence detection type electrophoresis apparatus
DE69221423T2 (de) * 1991-09-30 1998-03-19 Beckman Instruments Inc Verbesserte Fluoreszenz-Erfassung von Proben in einem Kapillarrohr

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9618099A1 *

Also Published As

Publication number Publication date
SE9404274D0 (sv) 1994-12-08
WO1996018099A1 (fr) 1996-06-13

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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