EP0801654A1 - N-SUBSTITUTED GLYCINE DERIVATIVES AS INHIBITORS OF FACTOR Xa - Google Patents

N-SUBSTITUTED GLYCINE DERIVATIVES AS INHIBITORS OF FACTOR Xa

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Publication number
EP0801654A1
EP0801654A1 EP95944234A EP95944234A EP0801654A1 EP 0801654 A1 EP0801654 A1 EP 0801654A1 EP 95944234 A EP95944234 A EP 95944234A EP 95944234 A EP95944234 A EP 95944234A EP 0801654 A1 EP0801654 A1 EP 0801654A1
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EP
European Patent Office
Prior art keywords
carbon atoms
substituted
compound according
mmole
alkyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP95944234A
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German (de)
English (en)
French (fr)
Inventor
Matthew Mark Abelman
Todd Anthony Miller
Ruth Foelsche Nutt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dendreon Pharmaceuticals LLC
Original Assignee
Corvas International Inc
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Priority claimed from US08/361,794 external-priority patent/US5696231A/en
Application filed by Corvas International Inc filed Critical Corvas International Inc
Publication of EP0801654A1 publication Critical patent/EP0801654A1/en
Withdrawn legal-status Critical Current

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    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07C311/01—Sulfonamides having sulfur atoms of sulfonamide groups bound to acyclic carbon atoms
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    • C07C317/50—Sulfones; Sulfoxides having sulfone or sulfoxide groups and carboxyl groups bound to the same carbon skeleton the carbon skeleton being further substituted by singly-bound nitrogen atoms, not being part of nitro or nitroso groups at least one of the nitrogen atoms being part of any of the groups, X being a hetero atom, Y being any atom
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    • C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/57—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups
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    • C07C323/57—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups
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    • C07C323/59—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups with amino groups bound to the carbon skeleton with acylated amino groups bound to the carbon skeleton
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    • C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/60—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton with the carbon atom of at least one of the carboxyl groups bound to nitrogen atoms
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    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D211/56—Nitrogen atoms
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    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
    • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D211/68—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having one double bond between ring members or between a ring member and a non-ring member
    • C07D211/72—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, directly attached to ring carbon atoms
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    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D213/54—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
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    • C07D451/00—Heterocyclic compounds containing 8-azabicyclo [3.2.1] octane, 9-azabicyclo [3.3.1] nonane, or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane or granatane alkaloids, scopolamine; Cyclic acetals thereof
    • C07D451/02—Heterocyclic compounds containing 8-azabicyclo [3.2.1] octane, 9-azabicyclo [3.3.1] nonane, or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane or granatane alkaloids, scopolamine; Cyclic acetals thereof containing not further condensed 8-azabicyclo [3.2.1] octane or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane; Cyclic acetals thereof
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    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
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    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
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    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06034—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms
    • C07K5/06043—Leu-amino acid
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    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
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    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/0606—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing heteroatoms not provided for by C07K5/06086 - C07K5/06139, e.g. Ser, Met, Cys, Thr
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    • C07K5/06—Dipeptides
    • C07K5/06086—Dipeptides with the first amino acid being basic
    • C07K5/06095—Arg-amino acid
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    • C07K5/06—Dipeptides
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    • C07K5/06139—Dipeptides with the first amino acid being heterocyclic
    • A—HUMAN NECESSITIES
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    • C07C2602/42—Systems containing two condensed rings the rings having more than two atoms in common the bicyclo ring system containing seven carbon atoms

Definitions

  • the present invention relates to compounds which are potent inhibitors of factor Xa.
  • the present invention relates to novel peptide aldehydes, their pharmaceutically acceptable salts, and pharmaceutically acceptable compositions thereof which are useful as potent inhibitors of blood coagulation in vitro and in vivo in mammals.
  • the invention relates to methods of using these inhibitors as therapeutic agents for disease states in mammals characterized by abnormal thrombosis.
  • the invention relates to methods of using these inhibitors as in vitro diagnostic agents.
  • the blood coagulation response is the culmination of a series of amplified reactions in which several specific zymogens of serine proteases in plasma are activated by limited proteolysis. This series of reactions results in the formation of an insoluble matrix composed of fibrin and cellular components which is required for the stabilization of the primary hemostatic plug or thrombus.
  • the initiation and propagation of the proteolytic activation reactions occurs through a series of amplified pathways which are localized to membranous surfaces at the site of vascular injury (Mann, K.G., Neshei , M.E., Church, W.R., Haley, P. and Krishnaswamy, S. (1990) Blood _6_: 1-16. and Lawson, J.H., Kalafatis, M. , Stra , S.,and Mann, K.G. (1994) J. Biol. Chem. 2&1 - 23357-23366).
  • Initiation of the blood coagulation response to vascular injury follows the formation of a catalytic complex composed of serine protease factor Vila and the non-enzymatic co-factor, tissue factor (TF) (Rappaport, S.I. and Rao, L.V.M. (1992) Arteriosclerosis and Thrombosis 12.: 1112-1121) .
  • tissue factor TF
  • This response appears to be exclusively regulated by the exposure of subendothelial TF to trace circulating levels of factor Vila and its zymogen factor VII, following a focal breakdown in vascular integrity.
  • Autoactivation results in an increase in the number of factor VIIa/TF complexes which are responsible for the formation of the serine protease factor Xa.
  • factor Xa primes the coagulation response through the proteolytic modification of factor IX to factor IXalpha which in turn is converted to the active serine protease factor IXa D by the factor
  • Factor Xa catalyzes the formation of thrombin following the assembly of the prothrombinase complex which is composed of factor Xa, the non-enzymatic co-factor Va and the substrate prothrombin (factor II) assembled in most cases, on the surface of activated platelets which are adhered at the site of injury (Fuster, V. , Badimon, L., Badimon, J.J. and Chesebro, J.H. (1992) New Engl. J. Med. 326: 310-318) .
  • the resulting amplified "burst" of thrombin generation catalyzed by prothrombinase results locally high levels of this protease which is responsible for the formation of fibrin and the further recruitment of additional platelets as well as the covalent stabilization of the clot through the activation of the transglutaminase zymogen factor XIII.
  • the coagulation response is further propagated through the thrombin- ediated proteolytic feedback activation of the non-enzymatic co-factors V and VIII resulting in more prothrombinase formation and subsequent thrombin generation (Hemker, H.C. and Kessels, H. (1991) Haemostasis 21 - 189-196) .
  • anticoagulants Substances which interfere in the process of blood coagulation (anticoagulants) have been demonstrated to be important therapeutic agents in the treatment and prevention of thrombotic disorders (Kessler, CM. (1991) Chest 22. - 97S-112S and Cairns, J.A. , Hirsh, J., Lewis, H.D., Resnekov, L. , and Theroux, P. (1992) Chest 102 : 456S-481S) .
  • the currently approved clinical anticoagulants have been associated with a number of adverse effects owing to the relatively non-specific nature of their effect on the blood coagulation cascade (Levine, M.N. , Hirsh, J.
  • a plasma protein, Lipoprotein- ssociated Coagulation Inhibitor (LACI) or recently termed Tissue Factor Pathway Inhibitor (TFPI) , containing three consecutive Kunitz domains has been reported to inhibit the enzyme activity of factor Xa directly and, in a factor Xa-dependent manner, inhibit the enzyme activity the factor Vila-tissue factor complex.
  • LACI Lipoprotein- ssociated Coagulation Inhibitor
  • TFPI Tissue Factor Pathway Inhibitor
  • Antistasin a protein comprised of 119 amino acids and found in the salivary gland of the Mexican leech, Haementeria officinalis, has been reported to inhibit the enzyme activity of factor Xa. Tuszynski et al. (1987) J. Biol. Chem, 262"9718; Nutt, et al. (1988) J. Biol. Chem, 263 :10162. A 6,000 daltons recombinant protein containing 58 amino acids with a high degree homology to antistasin's amino-terminus amino acids 1 through 58 has been reported to inhibit the enzyme activity of factor Xa. Tung, J. et al., EP 454,372 (1991); Tung, J. et al. , U.S. Patent No. 5,189,019 (1993) .
  • Tick Anticoagulant Protein (TAP) , a protein comprised of 60 amino acids and isolated from the soft tick,
  • Ornithodoros moubata has been reported to inhibit the enzyme activity of factor Xa but not factor Vila. axman, L. et al. (1990) Science, 24£:593. TAP made by recombinant methods has been reported. Vlausk, G.P. et al., EP 419,099 (1991) and Vlausk, G.P. et al. , U.S. Patent No 5,239,058 (1993).
  • A. caninum was reported to contain a substance which inhibited coagulation of blood in vitro. Loeb, L. and Smith, A.J. (1904) Proc. Pathol. Soc. Philadelphia, 2:173-178. Extracts of A. caninum were reported to prolong prothrombin time and partial thromboplastin time in human plasma with the anticoagulant effect being reported attributable to inhibition of factor Xa but not thrombin. Spellman, Jr., J.J. and Nossel, H.L. (1971) Am. J. Physiol., 22H -922-927.
  • the present invention is directed to novel compounds which are peptide argininals which include N-substituted glycine groups as part of the peptide backbone. These compounds are potent inhibitors of factor Xa in vivo and in vi tro .
  • the present invention is directed to compounds of the formula: wherein
  • Ri is selected from the group consisting of:
  • heterocycloalkyl of 4 to about 10 ring atoms with the ring atoms selected from carbon and heteroatoms, wherein the heteroatoms are selected from the group consisting of oxygen, nitrogen, and S(0)i, wherein i is 0, 1 or 2, optionally substituted on the ring with Y ] _, Y and/or Y3,
  • alkenyl of about 2 to about 6 carbon atoms which is optionally substituted with cyclic alkyl of about 5 to about 8 carbon atoms, which optionally is substituted on the ring carbons with Y * -_, Y 2 and/or Y3 ,
  • heteroaralkyl of 6 to 11 atoms with the ring atoms selected from carbon and heteroatoms, wherein the heteroatoms are selected from oxygen, nitrogen, and S(0) , and which is optionally substituted on the alkyl chain with hydroxy or halogen and optionally mono-, di- or tri-substituted on the ring with Y]_, Y 2 , and/or Y3 , respectively,
  • Yi and Y2 are selected together to be -0C(Z3) (Z4)0-, wherein Z and Z4 are independently selected from the group consisting of hydrogen, alkyl of 1 to about 12 carbon atoms, aryl of about 6 to about 14 carbon atoms, heteroaryl of about 5 to about 14 atoms having 1 to about 9 carbon atoms, aralkyl of about 7 to about 15 carbon atoms, and heteroaralkyl of about 6 to about 11 atoms having about 3 to about 9 carbon atoms, with the proviso that if X is not a direct link, then R- j _ is not hydrogen,
  • R 2 is selected from the group consisting of
  • Z5 is -OH, -OCH3, -OCH2CH3, or - R5R6, Zg is alkyl of 1 to about 4 carbon atoms, aryl of about 6 to about 14 carbon atoms, or aralkyl of about 7 to 16 carbon atoms,
  • R5 is hydrogen, or Z6,
  • R6 is hydrogen or cyclic alkyl of 3 to about 15 carbon atoms optionally mono- di- or tri-substituted with Y ] _, Y 2 and/or Y3 , aralkyl of about 7 to about 15 carbon atoms optionally mono-, di- or tri-substituted with Yi,
  • Y2 and/or Y3 heteroaryl of 5 to 14 atoms with the ring atoms selected from carbon and heteroatoms wherein the heteroatoms are selected from oxygen, nitrogen, and S(0) : , and which is optionally mono-, di- or tri-substituted with Yl- Y2 and/or Y3 , quinuclidine, or adamantyl,
  • - is 6, 7-dimethoxy-l, 2,3, 4- tetrahydroisoquinolinyl, 4-hydroxy piperidyl, 4-keto piperidyl, N-morpholino, 3 , 4-methylenedioxybenzyl piperazinyl, 4-phenyl piperazinyl optionally mono- substituted with fluoro, chloro, ethoxy, or trifluoromethyl, or 4-benzyl piperazinyl optionally mono- substituted with fluoro, chloro, methoxy, or trifluoromethy1, and pharmaceutically acceptible quaternary ammonium salts thereof;
  • R3 is selected from the group consisting of
  • R4 is selected from the group consisting of hydrogen, alkyl of 1 to about 7 carbon atoms optionally substituted with -OH or benzyloxy and alkyl of 1 to about 3 carbon atoms substituted on the terminal carbon atom with aryl of about 4 carbon atoms to about 10 carbon ato -j which is optionally mono-, di-, or tri-substituted with Yl, Y2 and/or Y3.
  • Peptidyl arginine aldehydes have been reported to exist in equilibrium structures in aqueous solutions. Bajusz, S., et al. (1990) J. Med. Chem., 3_3_: 1729.
  • the present invention is based on our finding that the novel compounds of our invention are active as potent inhibitors of factor Xa in vivo and in vitro.
  • certain of the preferred compounds of the present invention exhibit advantageous selectivity in that they are very potent inhibitors of factor Xa but are inactive or significantly less active, (several orders of magnitude less) in inhibiting plasmin and are significantly less active in inhibiting thrombin.
  • the present invention is directed to pharmaceutical compositions comprising a therapeutically effective amount of a compound of the present invention and a pharmaceutically acceptable carrier.
  • the present invention is directed to methods of using the compounds and pharmaceutical compositions of the present invention for the prevention of thrombosis in a mammal suspected of having a condition characterized by abnormal thrombosis, comprising administering to said mammal a therapeutically effective amount of a compound of the present invention or pharmaceutical composition comprising such a compound.
  • alkenyl refers to unsaturated aliphatic groups having at least one double bond.
  • alkyl refers to saturated aliphatic groups including straight-chain, branched-chain and cyclic groups.
  • alkoxy and alkoxyl refer to a group having the formula, R-0-, wherein R is an alkyl group.
  • alkoxycarbonyl refers to -C(0)OR wherein R is alkyl.
  • alkenyl refers to an alkenyl group substituted with an aryl group.
  • aralkyl refers to an alkyl group substituted with an aryl group. Suitable aralkyl groups include benzyl, picolyl, and the like, all of which may be optionally substituted.
  • aryl refers to aromatic groups which have at least one ring having a conjugated pi electron system and includes carbocyclic aryl, heterocyclic aryl and biaryl groups, all of which may be optionally substituted.
  • aryloxy refers to a group having the formula, R-O-, wherein R is an aryl group.
  • aralkoxy refers to a group having the formula, R-O-, wherein R is an aralkyl group.
  • amino acid refers to both natural, unnatural amino acids in their D and L stereoisomers if their structure allow such stereoisomeric forms, and their analogs.
  • Natural amino acids include alanine (Ala), arginine (Arg) , asparagine (Asn) , aspartic acid (Asp) , cysteine (Cys) , glutamine (Gin) , glutamic acid (Glu) , glycine (Gly) , histidine (His) , isoleucine (lie) , leucine (Leu) , lysine (Lys) , methionine (Met) , phenylalanine (Phe) , proline (Pro) , serine (Ser) , threonine (Thr) , tryptophan (Trp) , tyrosine (Tyr) and valine (Val) .
  • Unnatural amino acids include, but are not limited to azetidinecarboxylic acid, 2-aminoadipic acid, 3-aminoadipic acid, beta-alanine, aminopropionic acid, 2-aminobutyric acid, 4-aminobutyric acid, 6-aminocaproic acid, 2-aminoheptanoic acid, 2-aminoisobutyric acid, 3-aminoisobutyric acid, 2-aminopimelic acid, 2,4 diaminoisobutyric acid, desmosine, 2, 2 ' -diaminopimelic acid, 2, 3-diaminopropionic acid, N-ethylglycine, N-ethylasparagine, hydroxylysine, allo-hydroxylysine, 3-hydroxyproline, 4-hydroxyproline, isodesmosine, allo-isoleucine, N-methylglycine, N- methylisoleucine, N-methylvaline, norvaline, norleucine, ornith
  • Amino acid analogs include the natural and unnatural amino acids which are chemically blocked, reversibly or irreversibly, or modified on their N-terminal amino group or their side-chain groups, as for example, methionine sulfoxide, methionine sulfone, S- (carboxymethyl) -cysteine, S- (carboxymethyl) -cysteine sulfoxide and S- (carboxymethyl) -cysteine sulfone.
  • amino acid analog refers to an amino acid wherein either the C-terminal carboxy group, the N- terminal amino group or side-chain functional group has been chemically modified to another functional group.
  • aspartic acid- (beta-methyl ester) is an amino acid analog of aspartic acid
  • N-ethylglycine is an amino acid analog of glycine
  • alanine carboxamide is an amino acid analog of alanine.
  • amino acid residue refers to radicals having the structure: (1) -C(0)-R-NH-, wherein R typically is -CH(R')-, wherein R' is H or a carbon containing
  • aryl refers to phenyl substituted by carbocyclic or heterocyclic aryl as defined herein, ortho, meta or para to the point of attachment of the phenyl ring.
  • Carbocyclic aryl refers to aromatic groups wherein the ring atoms on the aromatic ring are carbon atoms.
  • Carbocyclic aryl groups include monocyclic carbocyclic aryl groups and naphthyl groups, all of which may be optionally substituted.
  • Suitable carbocyclic aryl groups include phenyl and naphthyl.
  • Suitable substituted carbocyclic aryl groups include indene and phenyl substituted by one to two substituents such being advantageously lower alkyl, hydroxy, lower alkoxy, lower alkoxycarbonyl, halogen, trifluoromethyl, nitro, and cyano.
  • Substituted naphthyl refers to 1- or 2-naphthyl substituted by lower alkyl, lower alkoxy, or halogen.
  • Cycloalkenyl refers to a cyclic alkenyl group. Suitable cycloalkenyl groups include, for example, cyclopentenyl and cyclohexenyl.
  • Cycloalkyl refers to a cyclic alkyl group. Suitable cycloalkyl groups include, for example, cyclohexyl, cyclopropyl, cyclopentyl, and cycloheptyl. “Cyclohexylmethyl” refers to a cyclohexyl group attached to CH2.
  • halogen refers to fluorine, chlorine, bromine and iodine.
  • Heteroaralkenyl refers to an alkenyl group substitued with a heteroaryl.
  • Heteroaralkyl refers to an alkyl group substituted with a heteroaryl.
  • Heteroaryl refers to aryl groups having from 1 to 9 carbon atoms and the remainder of the atoms are heteroatoms. Suitable heteroatoms include oxygen, nitrogen, S(0)j., wherein i is 0, 1 or 2, and suitable heterocyclic aryls include furanyl, thienyl, pyridyl, pyrrolyl, pyrimidyl, pyrazinyl, imidazolyl, and the like. “Heterocyclo” refers to a reduced heterocyclic ring system comprised of carbon, nitrogen, oxygen and/or sulfur atoms.
  • Heterocycloalkyl refers to an alkyl group substituted with a heterocyclo group, and includes those heterocyclic systems described in "Handbook of Chemistry and Physics", 49th edition, 1968, R.C. Weast, editor;The Chemical Rubber Co., Cleveland, OH. See particularly
  • lower referred to herein in connection with organic radicals or compounds defines such with up to and including 5, preferably up to and including 4 and advantageously one or two carbon atoms .
  • Such groups may be straight chain or branched chain.
  • Perfluoroalkyl refers to an alkyl group which has every hydrogen replaced with fluorine.
  • Perfluoroaryl refers to an aryl group which has every hydrogen replaced with fluorine.
  • Perfluoroaryl alkyl refers an aralkyl group in which every hydrogen on the aryl moiety is replaced with fluorine.
  • “Pharmaceutically acceptable salt” includes salts of the compounds of the present invention derived from the combination of such compounds and an organic or inorganic acid. In practice the use of the salt form amounts to use of the base form. The compounds of the present invention are useful in both free base and salt form, with both forms being considered as being within the scope of the present invention.
  • quaternary ammonium salt refers to compounds produced by reaction between a basic nitrogen in an R2 substituent and an alkylhalide, arylhalide, and aralkylhalide. Other reactants with good leaving groups may also be used, such as alkyl trifluoromethanesulfonates, alkyl methanesulfonates, and alkyl p-toluenesulfonates.
  • a quaternary ammonium salt has a positively charged nitrogen in the R2 substituent.
  • Counterions include C1-, Br-, I-, CF3C(0)0- and CH3C(0)0-.
  • the counterion of choice can be made using ion exchange resin columns.
  • R2 groups may contain basic nitrogens: -CH2S(0)2(CH2)pC(0)Z5, - (CH2) p S(0) 2Z6, - (CH2) p C(ONR5R6, and - (CH2) C(0) ' -X ⁇ ,wherein Z5, Z6, R5, R ⁇ ⁇ and __ are as defined in conjunction with formula I above.
  • R6 groups contain basic amines: 3-
  • Arg-al refers to the residue of L- argininal which has the formula:
  • N-alpha-t-butoxycarbonyl-N9-nitro-L- arginine refers to the compound which has the formula:
  • R2 groups with basic nitrogens include
  • R2 groups may contain basic nitrogens: -CH2S(0)2 (CH2)pC(0)Z5, -(CH2) p S(0)2Z6. -(CH2) C(0)NR5R6, and - (CH2) p C(0) ⁇ " ,wherein each Z5, ZQ ,
  • R5, R6, and ⁇ s is as defined in conjunction with formula I above.
  • R6 groups contain basic amines: 3-(R) -quinuclidine, 3-(S)-quinuclidine,
  • terminal carbon refers to the carbon atom of a straight chain alkyl which is furthest from the parent structure.
  • Boc or “BOC” refers to t-butoxycarbonyl.
  • BOP refers to benzotriazol-1-yl-oxy-tris-
  • DCC refers to N,N'-dicyclohexylcarbodiimide.
  • EDC refers to l-ethyl-3- (3-dimethylamino- propyl)carbodiimide hydrochloride salt.
  • HATU refers to 0- (7-azabenzotriazol-l-yl)-1, 1,3, 3- tetramethyluronium hexafluorophosphate.
  • HBTU refers to 2- (lH-benzotriazol-1-yl) -1, 1, 3,3- tetramethyluronium hexafluorophosphate.
  • HCl refers to hydrochloric acid.
  • HOAt refers to l-hydroxy-7-azabenzotriazole.
  • HBt refers to 1-hydroxybenzotriazole monohydrate.
  • HPLC refers to high pressure liquid chromatography.
  • LiAlH4 refers to lithium aluminum hydride.
  • LiAlH2 (0Et)2 refers to lithium aluminum dihydride diethoxide.
  • NMM refers to N-methylmorpholine.
  • NaOH refers to sodium hydroxide.
  • THF refers to tetrahydrofuran.
  • TBTU refers to 2- (lH-benzotriazol-1-yl) -1, 1,3,3- tetramethyluronium tetrafluoroborate.
  • TLC refers to thin layer chromatography
  • R' is hydrogen, alkyl of 1 to about 4 carbon atoms, aryl of about 6 to about 14 carbon atoms or aralkyl of about 6 to about 16 carbon atoms, and R" is NR', OR', R', or SR', with the proviso that R" is not NH, OH, H, or SH, and ;
  • Ri is selected from the group consisting of:
  • alkyl of 1 to about 12 carbon atoms optionally substituted with Y ] _ (2) alkyl of 1 to about 3 carbon atoms substituted with cyclic alkyl of about 5 to about 8 carbon atoms optionally substituted on the ring with Y_ , Y 2 and/or Y3 , (3) cyclic alkyl of 3 to about 15 carbon atoms, which optionally is substituted on the ring with Y 1( Y and/or Y3,
  • heterocycloalkyl of 4 to about 10 ring atoms with the ring atoms selected from carbon and heteroatoms, wherein the heteroatoms are selected from the group consisting of oxygen, nitrogen, and S(0)i, wherein i is 0, 1 or 2 , optionally substituted on the ring with Y-_ , Y and/or Y3, (5) heterocyclo of 4 to about 10 ring atoms with the ring atoms selected from carbon and heteroatoms, wherein the heteroatoms are selected from the group consisting of oxygen, nitrogen, and S(0)i, including
  • alkenyl of about 2 to about 6 carbon atoms which is optionally substituted with cyclic alkyl of about 5 to about 8 carbon atoms, which optionally is substituted on the ring carbons with Y- j _ , Y2 and/or Y3 ,
  • heteroaralkyl of 6 to 11 atoms with the ring atoms selected from carbon and heteroatoms, wherein the heteroatoms are selected from oxygen, nitrogen, and S(0)i, and which is optionally substituted on the alkyl chain with hydroxy or halogen and optionally mono-, di- or tri-substituted on the ring with Yi, Y , and/or Y3, respectively,
  • Yi and Y2 are selected together to be -OC(Z3) (Z4)0-, wherein Z3 and Z4 are independently selected from the group consisting of hydrogen, alkyl of 1 to about 12 carbon atoms, aryl of about 6 to about 14 carbon atoms heteroaryl of about 5 to about 14 atoms having 1 to about 9 carbon atoms, aralkyl of about 7 to about 15 carbon atoms, and heteroaralkyl of about 6 to about 11 atoms having about 3 to about 9 carbon atoms, with the proviso that if X is not a direct link, then R ] _ is not hydrogen,
  • R 2 is selected from the group consisting of
  • R5 is hydrogen, or Z ⁇ ,
  • R6 is hydrogen or cyclic alkyl of 3 to about 15 carbon atoms optionally mono- di- or tri-substituted with Yi, Y2 and/or Y3, aralkyl of about 7 to about 15 carbon atoms optionally mono-, di- or tri-substituted with Yi,
  • heteroaryl of 5 to 14 atoms with the ring atoms selected from carbon and heteroatoms wherein the heteroatoms are selected from oxygen, nitrogen, and S(0)i and which is optionally mono-, di- or tri-substituted with Yl, Y2, and/or Y3 , quinuclidine, or adamantyl,
  • __ is 6,7-dimethoxy-l,2,3,4- tetrahydroisoquinolinyl, 4-hydroxy piperidyl, 4-keto piperidyl, N-morpholino, 3, 4-methylenedioxybenzyl piperazinyl, 4-phenyl piperazinyl optionally mono- substituted with fluoro, chloro, methoxy, or trifluoromethyl, or 4-benzyl piperazinyl optionally mono- substituted with fluoro, chloro, methoxy, or trifluoromethyl, and pharmaceutically acceptible quaternary ammonium salts thereof;
  • R3 is selected from the group consisting of
  • aryl of about 3 to about 10 carbon atoms which is optionally mono-, di-, or tri-substituted with Yi, Y2 and/or Y3;
  • R4 is selected from the group consisting of hydrogen, alkyl of 1 to about 7 carbon atoms optionally substituted with -OH or benzyloxy and alkyl of 1 to about 3 carbon atoms substituted on the terminal carbon atom with aryl of about 4 carbon atoms to about 10 carbon atoms which is optionally mono-, di-, or tri-substituted with Yl, Y2 and/or Y3.
  • Preferred X groups include -SO2-, -NH-S(0)2", and -N(R' ) -S(0)2 • Especially preferred X groups include -SO2 •
  • Preferred Ri groups include alkyl, aralkyl, and aryl groups. Suitable aralkyl and aryl groups include substituted or unsubstituted benzyl and naphthyl, respectively. Preferred substitutions include, -C(0)OH, -C(0)OZ ⁇ , -S(0)mZ ⁇ , and -CF3. Meta and ortho substitution is preferred. Ortho substitution is particularly preferred.
  • Especially preferred Ri groups include aralkyl groups. Particularly preferred Ri groups include substituted or unsubstituted benzyl groups. Cyclohexyl and cyclohexylmethyl also are especially preferred Ri groups.
  • R5 groups include hydrogen.
  • Preferred R6 groups include hydrogen, 3-(R)- quinuclidine, 3- (S) -quinuclidine, 4-trifluoromethyl-7-yl- coumarin, 4-methyl-7 ⁇ yl-coumarin, 7-yl-coumarin, 3-yl-2- ethyl-4 (3H) -quinazolinone, 2-yl-benzothiazole, 3-yl- benzoic acid, 3-yl-4-hydroxybenzoic acid, 4-hydroxy-l- methyl-6-phenyl-3-yl-2 (1H) -pyridone, and 1-adamantyl, or ethyl morpholine, ethyl piperidine, 2- (2-ethyl)pyridine, 4-hydroxyphenethyl, (R) -alpha-methylbenzyl, (S)-alpha- methylbenzyl, 4- (methyl) -5-hydroxy-6-methyl-3-pyridine methanol, (1R,2S) - (N-methyl-N- (1
  • a preferred group of quaternary ammonium salts on the R2 group are those alkylated with alkyl of 1 to about 10 carbon atoms. Particularly preferred straight chain quaternary ammonium salts are those with methyl, ethyl, propyl and butyl .
  • Preferred branched alkyl quaternary ammonium salts include isopropyl, isobutyl, isopentyl, and isoamyl .
  • Preferred cyclic quaternary ammonium salts include cyclohexyl, cyclopentyl, and cyclohexylmethyl .
  • quaternary ammonium salts are those alkylated with aralkyl of 7 to about 15 carbon atoms .
  • Particularly preferred aralkyl groups for quaternary ammonium salts include benzyl and phenethyl .
  • Another preferred group of quaternary ammonium salts are those alkylated with aryl of 6 to about 14 carbon atoms, optionally substituted.
  • Preferred counterions to the quaternary ammonium salts include chlorine, bromine, iodine, acetate, and trifluoroacetate.
  • R3 groups include alkyl groups of 1 to about 7 carbon atoms optionally substituted with -OH on a terminal carbon atom.
  • Other preferred R3 groups include methyl, cyclohexylmethyl, phenyl, and benzyl. Particularly preferred R3 groups are methyl, and cyclohexyl.
  • R4 groups include hydrogen and alkyl groups of 1 to about 7 carbon atoms optionally substituted with -OH on a terminal carbon atom. Particularly preferred is hydrogen.
  • X is -S(0)2*-.
  • a very preferred aspect is directed to such compounds where Ri is substituted or unsubstituted benzyl.
  • Preferred compounds include D-camphorsulfonyl aspartyl sarcosine arginine aldehyde (Example 10) , D- camphorsulfonyl cysteinesulfone-acetic acid sarcosine- arginine aldehyde (Example 44) , D-camphorsulfonyl-L- methionine sulfone sarcosine-arginine aldehyde (Example 28) , benzylsulfonyl-D-arginine-sarcosine-arginine aldehyde (Example 16), (2-carbomethoxy)benzenesulfonyl- (D) - argininyl-sarcosine-argininal (Example 21) , N- benzylsulfonyl- (D) -methioninylsulfone sarcosine argininal (Exa
  • preferred compounds of formula (I) also include the compounds of the following Examples: 56 and 115A, 16 and 116A, 116C, 117, 115C, 118E, 115D, 116F, 108 and 120E, 114 and 120H, 113 and 120G, and 120J.
  • the present invention is directed to salts of the compounds of formula (I) .
  • Salt includes within its definition, salts of the compounds of the present invention derived from the combination of such compounds and an organic or inorganic acid.
  • the use of the salt form amounts to use of the base form.
  • the compounds of the present invention are useful in both free base and salt form, with both forms being considered as being within the scope of the present invention.
  • These salts include acid addition salts, for example, salts of hydrochloric acid, hydrobromic acid, acetic acid, benzene sulfonic acid and other suitable acid addition salts.
  • These salts include salts formed from compounds containing quaternary ammonium salts.
  • the compounds of the present invention may be prepared by the preferred reaction schemes depicted in Figures 1, 3, 4, and 5.
  • Examples 1 through 4 provide the details of a preferred method of making the commonly utilized intermediate, N-3-nitro-L-argininal ethyl cyclol.
  • various Boc protected amino acids are coupled to sarcosine benzyl ester.
  • Other N- alkylated amino acids may be used.
  • the Boc group is removed with hydrochloric acid.
  • the hydrochloride salt of the terminal free amine is reacted with triethylamine, and R1SO2CI in acetonitrile to give the sulfonamide, which is then hydrogenated with hydrogen gas and palladium on carbon in a Parr Shaker to remove the benzyl ester protecting group.
  • the free acid is coupled to N9-nitro-L- argininal ethyl cyclol hydrochloride salt (prepared as described in Examples 1 through 4) by carbodiimide coupling.
  • the 9-nitro group of the adduct is then removed by hydrogenation with hydrogen gas and palladium on carbon in ethanol, water, and acetic acid. Then the compound is reacted with 3N hydrochloric acid or hexafluorophosphoric acid to provide the argininal compound of the invention.
  • Preferred means of chemically coupling include formation of a peptide bond by using conventional coupling reagents known in the art. See Bodanszky, N., Peptide Chemistry, pp. 55-73, Springer-Verlag, New York (1988) and references cited therein.
  • the chemical coupling may be either by means of one-step or two-step coupling. In one-step coupling, the two coupling partners are coupled directly.
  • Preferred coupling reagents for one-step coupling include N,N' -dicyclohexylcarbodiimide with 1-hydroxybenzotriazole monohydrate, l-ethyl-3- (3-dimethylaminopropyl)carbodiimide with 1-hydroxybenzotriazole monohydrate, benzotriazol-1- yl-oxy-tris (dimethylamino)phosphonium hexafluorophosphate, 2- (lH-benzotriazol-1-yl) -1,1,3, 3-tetramethyluronium hexafluorophosphate or 2- (lH-benzotriazol-1-yl) -1, 1, 3 , 3- tetramethyluronium tetrafluoroborate.
  • an activated ester or anhydride of the C- terminal carboxy group of one coupling partner is formed prior to its coupling to the other coupling partner.
  • an even more preferred method is to use the di-N-t- butoxycarbony1 protecting group for the L-argininal moiety for groups incompatible with hydrogenation with palladium on carbon.
  • alpha-N-t-benzyloxycarbonyl- omega, omega'-di-N-t-butoxycarbonylarginine is dissolved in acetonitrile and treated with hydroxybenzotriazole and l-ethyl-3- (3-dimethylamino-propyl)carbodiimide hydrochloric acid salt to form alpha-N-benzyloxycarbonyl- omega, omega'-di-N-t-butoxycarbonyl-L-arginine lac arn.
  • the lactam is opened by treatment with LiAlH4 in tetrahydrofuran at -70°C to provide alpha-N- benzyloxycarbonyl-omega, omega'-di-N-t-butoxycarbonyl-L- argininal.
  • This aldehyde is protected as the diethyl acetal by treatment with ethanol and hydrochloric acid.
  • the N-benzyloxycarbonyl protecting group is removed by treatment with hydrogen gas and palladium on carbon to give omega, omega'-di-N-t-butoxycarbonyl-L-argininal diethyl acetal, hydrochloric acid salt.
  • This protected L- argininal moiety can then be coupled to a desired carboxylic acid by treatment with N-hydroxybenzotriazole and l-ethyl-3- (3-dimethylamino-propyl)carbodiimide hydrochloric acid salt.
  • the diethyl acetal and the di-BOC protecting groups are removed by treatment with hexafluorophosphonic acid in acetonitrile at 0°C.
  • the reaction is quenched with 2.5 M aqueous sodium acetate until pH 4 is reached.
  • the mixture is filtered through a 2 micron filter.
  • Preparative HPLC using 0.1% CF3COOH in 10-40% aqueous acetonitrile provides the trifluoroacetate salt of the desired substituted L-argininal compound.
  • the Boc protected methionine sulfone sarcosine benzyl ester is N- alkylated with (2-iodoethyl) benzene.
  • Various N- alkylating agents can be used.
  • the benzyl ester protecting group is removed by treatment with hydrogen gas and palladium on carbon.
  • the resulting free acid is coupled to the compound of Example 4 by treatment with 1- ethyl-3 (-dimethylaminopropyl)carbodiimide, N- hydroxybenzotriazole, and N-methyl morpholine.
  • the N9- nitro group is removed by treatment with hydrogen gas, palladium on carbon in methanol, acetic acid and water mixture.
  • FIG. 3 depicts a scheme for the synthesis of compounds where X is a direct link. Examples 74a through 78 describe such a synthesis.
  • Figure 4 illustrates a preferred method of synthesizing compounds with an N-alkylated R2 group.
  • N- alkylated encompasses N-alkylated, N-arylated, and N- aralkylated quaternary ammonium salts. Examples 79 through 89 provide the details of this synthetic scheme.
  • the Boc protected glumatic acid benzyl ester is coupled to 3- (R) -aminoquinuclidine dihydrochloride using standard procedures.
  • Various amines can be used. Strong acid removes the Boc protecting group.
  • the amine is then reacted with triethylamine and benzylsulfonyl chloride.
  • the benzyl ester protecting group is removed by catalytic hydrogenation.
  • the free acid is then coupled to the compound of Example 4 using standard coupling reagents.
  • the basic amine of the R2 group is then reacted with allyliodide.
  • the N ⁇ -nitro group is removed and the allyl group reduced by treatment with hydrogen gas and palladium on carbon.
  • the cyclic arginine ethylaminal is opened with hexafluorophosphoric acid or 6 M hydrochloric acid in water to give the final product.
  • Figure 5 illustrates a preferred reaction scheme for the preparation of N-benzylsulfonyl-L-cysteine sulfone-S-
  • Boc group of butyl acetate cysteinesulfone sarcosine-O- benzyl ester prepared according to Examples 36 through 39 is removed by treatment with anhydrous hydrochloric acid.
  • the terminal amine is reacted with an amine base and R1SO2CI to provide the sulfonamide.
  • the t-butyl ester is reacted with trifluoroacetic acid to form the carboxylic acid.
  • the acid is then coupled to (R) -alphamethyl benzylamine using l-ethyl-3- (3- dimethylaminopropyl)carbodiimide hydrochloride salt and 1- hydroxybenzotriazole.
  • the resulting adduct is then treated with hydrogen gas and palladium on carbon to remove the benzyl ester protecting group.
  • the resulting acid is coupled to the compound of Example 4 using standard coupling techniques.
  • the N ⁇ -nitro group is removed with catalytic hydrogenation and then the argininal ethyl cyclol aminal is opened with hexafluorophosphoric acid or 6 M hydrochloric acid in water to give the argininal product.
  • the compounds of the present invention are screened for their ability to inhibit thrombin, plasmin, recombinant tissue plasminogen activator (rt-PA) , activated protein C (aPC) , chymotrypsin, and trypsin as set forth below. Certain of the preferred compounds are distinguished by their ability to inhibit factor Xa, while not substantially inhibiting thrombin, plasmin, t-PA, aPC, chymotrypsin, and trypsin.
  • the term "not substantially inhibiting" means that the IC50 (or Kj for thrombin, plasmin, t-PA, aPC, chymotrypsin, and trypsin for a given compound is greater than or equal to its IC50 (or Ki, respectively) for factor Xa.
  • the compounds of the present invention are dissolved in buffer to give solutions containing concentrations such that assay concentrations range from 0 to 100 micromolar.
  • concentrations such that assay concentrations range from 0 to 100 micromolar.
  • a chromogenic synthetic substrate is added to a solution containing test compound and the enzyme of interest and the residual catalytic activity of that enzyme is determined spectrophometrically.
  • the IC 50 of a compound of the present invention is determined from the rate of substrate turnover caused by the specific enzyme being measured. IC50 is that concentration of test compound giving 50% inhibition of the rate of substrate turnover.
  • Ki of a compound of the present invention is determined from the rate of substrate turnover caused by the specific enzyme being measured at various enzyme concentrations. Ki is that concentration of test compound giving 50% inhibition of the rate of substrate turnover.
  • Examples A and B provide an exemplar of the in vitro assays used to select the compounds of the present invention.
  • Certain of the preferred compounds of the present invention have a Ki of about 0.001 to about 200 nM in the factor Xa assay. Especially preferred compounds have a Ki of about 0.001 to about 50 nM. The more especially preferred compounds have a Ki of about 0.001 to about 10 nM.
  • Certain of the preferred compounds of the present invention have a IC50 for thrombin, plasmin, t-PA, aPC, chymotrypsin, and trypsin which is at least 10 times greater than its IC50 for factor Xa.
  • Especially preferred compounds have an IC50 for thrombin, plasmin, rt-PA, aPC, chymotrypsin, and trypsin which is about 20 to about 100,000 times greater than its IC50 for thrombin. More especially preferred compounds have an IC50 for thrombin, plasmin, rt-PA, aPC, chymotrypsin, and trypsin which is about 100 to about 1,000,000 times greater than its IC50 for factor Xa.
  • a compound of the present invention has an IC50 with respect to thrombin, plasmin, rt-PA, aPC, chymotrypsin, or trypsin which is greater than the highest concentration of compound tested, the IC50 is taken to be that highest concentration of compound. 4.
  • the present invention encompasses pharmaceutical compositions prepared for storage or administration which comprise a therapeutically effective amount of a compound of the present invention in a pharmaceutically acceptable carrier.
  • the therapeutically effective amount of a compound of the present invention will depend on the route of administration, the type of mammal being treated, and the physical characteristics of the specific mammal under consideration. These factors and their relationship to determining this amount are well known to skilled practitioners in the medical arts. This amount and the method of administration can be tailored to achieve optimal efficacy but will depend on such factors as weight, diet, concurrent medication and other factors which those skilled in the medical arts will recognize.
  • the therapeutically effective amount of a compound of the present invention can range broadly depending upon the desired effects and the therapeutic indication.
  • dosages will be between about 0.01 mg/kg and 100 mg/kg body weight, preferably between about 0.01 and 10 mg/kg, body weight.
  • Pharmaceutically acceptable carriers for therapeutic use are well known in the pharmaceutical art, and are described, for example, in Remington's Pharmaceutical Sciences. Mack Publishing Co. (A.R. Gennaro edit. 1985) .
  • sterile saline and phosphate-buffered saline at physiological pH may be used.
  • Preservatives, stabilizers, dyes and even flavoring agents may be provided in the pharmaceutical composition.
  • sodium benzoate, sorbic acid and esters of p- hydroxybenzoic acid may be added as preservatives. Id. at 1449.
  • antioxidants and suspending agents may be used. Id.
  • compositions of the present invention may be formulated and used as tablets, capsules or elixirs for oral administration; suppositories for rectal administration; sterile solutions and suspensions for injectable administration; and the like.
  • the dose and method of administration can be tailored to achieve optimal efficacy but will depend on such factors as weight, diet, concurrent medication and other factors which those skilled in the medical arts will recognize.
  • injectable pharmaceutical compositions can be prepared in conventional forms, either as liquid solutions or suspensions, solid forms suitable for solution or suspension in liquid prior to injection, or as emulsions.
  • Suitable excipients are, for example, water, saline, dextrose, mannitol, lactose, lecithin, albumin, sodium glutamate, cysteine hydrochloride, or the like.
  • the injectable pharmaceutical compositions may contain minor amounts of nontoxic auxiliary substances, such as wetting agents, pH buffering agents, and the like.
  • absorption enhancing preparations e.g., liposomes may be utilized.
  • Compounds of the present invention when made and selected as disclosed are useful as potent inhibitors of factor Xa in vitro and in vivo. As such, these compounds are useful as in vitro diagnostic reagents to prevent the clotting of blood and as in vivo pharmaceutical agents to prevent thrombosis in mammals suspected of having a condition characterized by abnormal thrombosis.
  • the compounds of the present invention are useful as in vitro diagnostic reagents for inhibiting clotting in blood drawing tubes.
  • stoppered test tubes having a vacuum therein as a means to draw blood obtained by venipuncture into the tube is well known in the medical arts. Kasten, B.L., "Specimen Collection", Laboratory Test Handbook. 2nd Edition, Lexi-Co p Inc., Cleveland pp. 16-17 (Edits. Jacobs, D.S. et al . 1990) .
  • Such vacuum tubes may be free of clot-inhibiting additives, in which case, they are useful for the isolation of mammalian serum from the blood.
  • clot-inhibiting additives such as heparin salts, EDTA salts, citrate salts or oxalate salts
  • clot-inhibiting additives such as heparin salts, EDTA salts, citrate salts or oxalate salts
  • the compounds of the present invention are potent inhibitors of factor Xa, and as such, can be incorporated into blood collection tubes to prevent clotting of the mammalian blood drawn into them.
  • the compounds of the present invention are used alone, in combination of other compounds of the present invention, or in combination with other known inhibitors of clotting, in the blood collection tubes.
  • the amount to be added to such tubes is that amount sufficient to inhibit the formation of a clot when mammalian blood is drawn into the tube.
  • the addition of the compounds to such tubes may be accomplished by methods well known in the art, such as by introduction of a liquid composition thereof, as a solid composition thereof, or liquid composition which is lyophilized to a solid.
  • the compounds of the present invention are added to blood collection tubes in such amounts that, when combined with 2 to 10 mL of mammalian blood, the concentration of such compounds will be sufficient to inhibit clot formation. Typically, the required concentration will be about 1 to 10,000 nM, with 10 to 1000 nM being preferred.
  • the compounds of the present invention are useful as pharmaceutical agents for preventing thrombosis in a mammal suspected of having a condition characterized by abnormal thrombosis.
  • Conditions characterized by abnormal thrombosis are well known in the medical arts and include those involving the arterial and venous vasculature of mammals.
  • abnormal thrombosis thrombus formation
  • PTCA percutaneous transluminal coronary angioplasty
  • abnormal thrombosis characterizes the condition observed in patients undergoing major surgery in the lower extremities or the abdominal area who often suffer from thrombus formation in the venous vasculature resulting in reduced blood flow to the affected extremity and a predisposition to pulmonary embolism.
  • Abnormal thrombosis further characterizes disseminated intravascular coagulopathy which commonly occurs within both vascular systems during septic shock, certain viral infections and cancer, a condition wherein there is rapid consumption of coagulation factors and systemic coagulation which results in the formation of life- threatening thrombi occurring throughout the microvasculature leading to widespread organ failure.
  • the present invention includes methods for preventing a condition in a mammal suspected of having a condition characterized by abnormal thrombosis, comprising administering to said mammal a therapeutically effective amount of a compound or a pharmaceutical composition of the present invention.
  • the compounds or pharmaceutical compositions of the present invention are administered in vivo, ordinarily in a mammal, preferably in a human.
  • the compounds or pharmaceutical compositions can be administered to a mammal in a variety of ways, including orally, parenterally, intravenously, subcutaneously, intramuscularly, colonically, rectally, nasally or intraperitoneally, employing a variety of dosage forms.
  • Administration is preferably parenteral, such as intravenous on a daily basis.
  • administration is preferably oral, such as by tablets capsules or elixirs taken on a daily basis.
  • the compounds or pharmaceutical compositions of the present invention are administered alone or in combination with one another, or in combination with other therapeutic or in vivo diagnostic agents.
  • a therapeutically effective amount of the compounds or pharmaceutical compositions of the present invention will vary depending upon the age, weight and mammalian species treated, the particular compounds employed, the particular mode of administration and the desired effects and the therapeutic indication. Because these factors and their relationship to determining this amount are well known in the medical arts, the determination of therapeutically effective dosage levels, the amount necessary to achieve the desired result of preventing thrombosis, will be within the ambit of one skilled in these arts. Typically, administration of the compounds or pharmaceutical compositions of the present invention is commenced at lower dosage levels, with dosage levels being increased until the desired effect of preventing in vivo thrombosis is achieved which would define a therapeutically effective amount. For the compounds of the present invention, alone or as part of a pharmaceutical composition, such doses are between about 0.01 mg/kg and 100 mg/kg body weight, preferably between about 0.01 and 10 mg/kg, body weight.
  • N-alpha-t-butoxycarbonyl-N ⁇ -nitroarginine (2.00 g, 6.3 mmole) was dissolved in tetrahydrofuran (100 mL) by heating the solution to 50°C. The solution was allowed to cool to room temperature. N-methylpiperidine (0.84 mL, 6.9 mmole) was added, and the solution was cooled in an ice bath. Isobutylchloroformate (0.83 mL, 6.3 mmole) was added, and the reaction mixture was stirred at 0°C for 6 hours. The reaction mixture was stirred for 18 hours while the ice in the dewar was allowed to melt overnight. The solvent was removed under vacuum.
  • a 12 liter four-necked round bottom flask equipped with an overhead stirring apparatus was flame dried under a strong stream of nitrogen. After the flask had cooled,
  • the crude intermediate was taken up in 300 L of dichloromethane and applied to a column of silica gel (70- 230 mesh, 7x50 cm) .
  • the column was first eluted with 2 liters of dichloromethane followed by 2 liters of 2% methanol in dichloromethane. This was followed by elution with 5% methanol in dichloromethane until all of the product had been eluted (the eluent was checked for UV activity and five one-liter fractions were collected once this UV activity was apparent) .
  • reaction When the reaction was judged to be complete, it was cooled to -70°C and 503 mL of 2M potassium bisulfate was added via dropping funnel at a slow enough rate to keep the reaction temperature below -30°C. The cooling bath was removed and the reaction mixture was allowed to come to 0°C over the course of 2 hours at which time a white precipitate was filtered off. The solids were washed with 500 mL of cold tetrahydrofuran. The filtrate was concentrated under vacuum on a rotary evaporator until most of the tetrahydrofuran was removed and the remaining white sludge was mostly aqueous.
  • the crude product was taken up in 1.5 liters of ethyl acetate and washed with 0.2 M hydrochloric acid (2 x 200 mL) .
  • the hydrochloric acid extracts were back-extracted with 400 mL of ethyl acetate and the organics were combined and extracted with saturated sodium bicarbonate (2 x 200 mL) .
  • the bicarbonate extracts were also back-extracted with 400 mL of ethyl acetate.
  • the organics were then combined and washed with brine (200 mL) followed by drying over anhydrous sodium sulfate.
  • Boc-D-N ⁇ -nitroarginine (6.2 g, 19.4 mmole) in 100 mL of dry dimethylformamide was added sarcosine benzyl ester para-toluenesulfonic acid salt (8.2 g, 23.3 mmole) followed by benzotriazol-1-yl- oxy-tris- (dimethylamino)-phosphonium hexafluorophosphate (8.6 g, 19.4 mmole) and N-methylmorpholine (10.6 mL, 97.1 mmole) . The mixture was stirred for 16 hours at room temperature.
  • the resulting oil was dissolved in dichloromethane (50 mL) and treated with 50 mL of 4.0M solution of hydrochloric acid in dioxane. After 5 hours, the title compound precipitated when the reaction mixture was poured into ether (500 mL) with vigorous stirring. The precipitate was filtered and dried in vacuo to provide 8 g (quantitative yield) of the title compound as an off-white powder.
  • Example 4 The compound of Example 4 (1.64 g, 6.1 mmole) and the the compound of Example 7 (2 g, 4.1 mmole) were dissolved with stirring in 20 mL of dry acetonitrile. To this mixture was added l-hydroxy-7-azabenzotriazole (0.28 g, 2.0 mmole) and O- (7-azabenzotriazol-l-yl) -1, 1, 3, 3- tetramethyluronium hexafluorophosphate (1.5 g, 4.1 mmole) followed by N-methylmorpholine (1.7 mL, 15.8 mmole) .
  • Example 9 The compound of Example 9 (1 g, 1.7 mmole) was dissolved in 20 mL of 50:50 water-.acetonitrile with stirring and cooled to 0°C in an ice water bath. To this solution was slowly added 50 mL of a 60 wt% solution of hexafluorophosphoric acid in water. After 1 hour, the pH of the reaction mixture was adjusted to about pH 4 using saturated aqueous sodium acetate. This mixture was filtered through a plug of Celite.
  • the title compound was obtained by purification of the filtrate by preparative HPLC (2 inch Vydak C18 column using a gradient consisting of 7 to 28% acetonitrile in water containing 0.1% trifluoroacetic acid run over 60 minutes at a flowrate of 115 mL/minute) and lyophilization of the pooled fractions.
  • Mass Spec(FAB) confirmed the calculated molecular weight of 598.7.
  • the resulting oil was dissolved in dichloromethane (500 mL) and treated with 100 mL of 4.0M solution of hydrochloric acid in dioxane. After 12 hours, the title compound was precipitated by pouring the reaction mixture into diethyl ether (500 mL) with vigorous stirring. The precipitate was filtered and dried in vacuo to provide 15 g (95% yield) of the title compound as an off-white powder.
  • Example 4 The compound of Example 4 (2.25 g, 8.42 mmole) and the compound of Example 13 (3.12 g, 7.02 mmole) were dissolved with stirring in 10 mL of dry dimethylformamide and 30 mL of dry acetonitrile. To this mixture was added l-ethyl-3- (3-dimethylamino-propyl)carbodiimide hydrochloride salt (2.02 g, 10.5 mmole) and 1- hydroxybenzotriazole monohydrate (1.42 g, 10.5 mmole), followed by diisopropylethylamine (6 mL, 4.5 mmole) .
  • Example 15 The compound of Example 15 (2.8 g, 5 mmole) was dissolved in 40 mL water and cooled to 0°C in an ice water bath with stirring. To this solution was slowly added 40 mL of concentrated hydrochloric acid. After 1.5 hour, the reaction was judged complete by HPLC and the pH of the reaction mixture was adjusted to about pH 4 using saturated aqueous sodium acetate. This mixture was filtered through a plug of Celite.
  • the title compound was obtained by purification from the filtrate by preparative HPLC (2 inch Vydak C18 column using a gradient consisting of 5 to 17% acetonitrile in water containing 0.1% trifluoroacetic acid run over 55 minutes at a flowrate of 115 mL/minute) and lyophilization of the pooled fractions.
  • Mass Spectroscopy (FAB) confirmed the calculated molecular weight of 539.6.
  • Example 18 The compound of Example 18 (1.0 g, 2.0 mmole) and the compound of Example 4 (0.66 g, 2.5 mmole) were suspended in acetonitrile (20 mL) , followed by l-ethyl-3- (3- dimethylamino-propyl)carbodiimide hydrochloride salt (0.48 g, 2.5 mmole) and 1-hydroxybenzotriazole monohydrate (0.34 g, 2.5 mmole). The reaction mixture was stirred for 30 minutes, then N-methylmorpholine (0.70 L, 6.0 mmole) was added.
  • Example 19 The compound of Example 19 (0.77 g, 1.1 mmole) was dissolved in methanol (20 mL) , acetic acid (5.0 mL) , and distilled water (5.0 mL) , and the solution was placed in a 250 mL Parr bottle. The vessel was purged with argon and then 10% palladium on carbon catalyst (0.3 g) was added. The reaction mixture was shaken under hydrogen (50 psi) for 2.5 days, then was filtered through Celite and concentrated under vacuum to give the title compound as an off-white foam.
  • Example 20 The compound of Example 20 (0.3 g, 0.49 mmole) was dissolved in 6N hydrochloric acid (8.0 mL, 48 mmole) at 0°C. The reaction mixture was stirred at 0°C for 4 hours, then at room temperature for 1.5 hours. The reaction mixture was recooled to 0°C, and saturated sodium acetate (20 mL) was added (to pH 4) . Purification on reverse phase HPLC, followed by lyophilization, gave the product as a white powder. Fast atom bombardment mass spectrometry confirmed the theoretical molecular weight of 583.
  • Example 24 To a solution of the compound of Example 22
  • Example 23 To a solution of the compound of Example 23 (3.7 g, 9.4 mmole) in 47 mL of dimethylformamide, was added with stirring sarcosine-O-benzyl ester para-toluenesulfonate salt (3.3 g, 9.4 mmole), followed by N-methylmorpholine (5.1 mL, 47 mmole) and benzotriazol-1-yl-oxy-tris- (dimethylamino) -phosphonium hexafluorophosphate (5.4 g, 9.4 mmole) . The stirring was continued for 16 hours.
  • sarcosine-O-benzyl ester para-toluenesulfonate salt 3.3 g, 9.4 mmole
  • N-methylmorpholine 5.1 mL, 47 mmole
  • reaction mixture was dissolved in 700 mL of ethyl acetate and washed with 250 mL each of water, 1 M aqueous hydrochloric acid, water, saturated aqueous sodium bicarbonate and brine.
  • the organic phase was dried over anhydrous magnesium sulfate, filtered and the solvent removed in vacuo to provide 5.16 g (96% yield) of the title compound as an off-white foam.
  • Rf 0.3 (1:9 methanol :dichloromethane) .
  • Example 24 To a solution of the compound of Example 24 (5.16 g, 9.01 mmole) in 300 mL of methanol under a nitrogen blanket, was added 10% palladium on carbon (4 g) and the mixture was hydrogenated at 1 atmosphere for 16 hours.
  • Example 4 The compound of Example 4 (0.85 g, 3.1 mmole) was dissolved with stirring in 6 mL of dry dimethylformamide and 16 mL of dry acetonitrile. To this mixture was added N-methylmorpholine (1.7 mL, 15.8 mmole) followed by the compound of Example 25 (1.15 g, 2.38 mmole), 1- hydroxybenzotriazole monohydrate (0.65 g, 4.7 mmole) and 2- (lH-benzotriazol-1-yl) -1,1,3, 3-tetramethyluronium hexafluorophosphate (1.8 g, 4.7 mmole) .
  • Example 27 The compound of Example 27 (0.5 g, 0.73 mmole) was dissolved in 20 mL of 50:50 water:acetonitrile with stirring and cooled to 0°C in an ice water bath. To this solution was slowly added 30 mL of a 60 wt% solution of hexafluorophosphoric acid in water. After 1 hour, the pH of the reaction mixture was adjusted to about pH 4 using saturated aqueous sodium acetate. This mixture was filtered through a plug of Celite.
  • N-Boc-(D)-methionine (17.1 g, 68.58 mmole) and sarcosine benzyl ester tosylate salt (24.08 g, 68.60 mmole) were suspended in 110 mL of acetonitrile and 25 mL of dimethylformamide at 0°C, then benzotriazol-1-yl-oxy- tris-(dimethylamino) -phosphonium hexafluorophosphate (30.34 g, 68.58 mmole) and N-methylmorpholine (20.83 g, 205.83 mmole) were added. The ice bath was removed after 30 minutes and the reaction was stirred for 18 hours at room temperature.
  • Example 29 The compound of Example 29 (26.0 g, 63.3 mmole) was dissolved in 335 mL of glacial acetic acid. Sodium perborate tetrahydrate (48.7 g, 316.5 mmole) was added and the mixture was heated to 55°C. After 2.5 hours at this temperature, the reaction mixture was diluted with 1.1 liters of brine, the aqueous layer was extracted with ethyl acetate (4x250 mL) and the combined organic extracts were dried with anhydrous magnesium sulfate. This solution was filtered and evaporated under vacuum, then repeatedly azeotroped with toluene (200 mL) under vacuum to remove acetic acid.
  • Example 31 The compound of Example 31 (4.42 g, 8.9 mmole) was dissolved in tetrahydrofuran (200 mL) , 0.8 g of 10% palladium on carbon was added and the mixture was stirred under hydrogen gas at atmospheric pressure for 18 hours. After the catalyst was filtered off the reaction mixture, the solvent was removed under vacuum and the resulting oil was taken up in a solution of saturated sodium bicarbonate. This solution was then extracted with ethyl acetate (1x150 mL) and the organic layer was decanted off. The remaining aqueous layer was layered with 100 mL of ethyl acetate and acidified with 3N hydrochloric acid to pH 2 (pH papers) .
  • Example 32 The compound of Example 32 (1.22 g, 3 mmole) and the compound of Example 4 (1.6 g, 6 mmole) were suspended in 15 mL of acetonitrile and 9 mL of dimethylfor-mamide, then 1-hydroxybenzotriazole monohydrate (0.61 g, 4.5 mmole), 2-(lH-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (1.71 g, 4.5 mmole) and N- methylmorpholine (1.5 g, 15 mmole) were added. The reaction mixture was stirred for 3 days at room temperature. The reaction mixture was reduced in volume under vacuum at 25°C to give an oil.
  • Example 33 The compound of Example 33 (0.50 g, 0.80 mmole) was dissolved in 40 mL of methanol, 7 mL of water and 0.3 mL of glacial acetic acid. 0.25 g of 10% Palladium on carbon catalyst was added to the solution and the mixture was placed on a PARR shaker at 40 psi hydrogen for 18 hours at room temperature, at which point the starting material was consumed. The catalyst was filtered and the reaction solution was reduced in volume under vacuum at 25°C to leave an oil. Toluene was added and evaporated several times, then methanol was added and evaporated to yield 0.48 g (95 %) of the title compound as a white solid.
  • Example 34 The compound of Example 34 (0.48 g, 0.75 mmole) was placed in a plastic reaction vessel and dissolved by addition of 5 mL of acetonitrile and 5 mL of deionized water. The solution was cooled to 0°C, then 10 mL of hexafluorophosphoric acid (60% wt. in water) at 0°C was added. The reaction mixture was stirred at 0°C for 2.0 hours at which time all the starting material was consumed. The reaction was quenched with 100 mL of 2.5 M sodium acetate, this raised the pH to pH 5.
  • Example 36 The compound of Example 36 (82.5 g, 341.7 mmole) and sodium bicarbonate (33.96 g, 404 mmole) were suspended in 600 mL of deionized water. A solution of di-t-butyl dicarbonate (80.88 g, 370 mmole) in 350 mL of dioxane was added and the slurry was stirred for 18 hours. The slurry was extracted with diethyl ether (2x100 L) . The slurry was layered with ethyl acetate (200 mL) and acidified with IN hydrochloric acid to pH 2 (pH papers) .
  • Example 37 The compound of Example 37 (57.32 g, 170.9 mmole) and sarcosine benzyl ester tosylate salt (60.0 g, 170.9 mmole) were suspended in 300 mL of acetonitrile and 60 mL of dimethylformamide at 0°C, then benzotriazol-1-yl-oxy-tris- (dimethylamino)-phosphonium hexafluorophosphate (75.2 g, 170.9 mmole) and N-methylmorpholine (51.9 g, 512.7 mmole) were added. The ice bath was removed after 30 minutes and the reaction was stirred for 48 hours at room temperature. The reaction mixture was reduced in volume under vacuum at 25 * C to give an oil.
  • Example 38 The compound of Example 38 (55.6 g, 111.96 mmole) was dissolved in 580 mL of glacial acetic acid and then sodium perborate tetrahydrate (86.1 g, 559.8 mmole) was added. This mixture was heated to 55"C. After 2.5 hours at this temperature, the reaction mixture was diluted with 1 liter of brine, the aqueous layer was extracted with ethyl acetate (4x250 mL) , and the combined organic extracts were dried with anhydrous magnesium sulfate. This solution was filtered and evaporated under vacuum, then repeatedly azeotroped with toluene (200 mL) under vacuum to remove acetic acid.
  • Example 39 The compound of Example 39 (3.9 g, 9.22 mmole) and (D)-camphorsulfonyl chloride (3.5 g, 13.8 mmole) were dissolved with stirring in 20 mL of dry dimethylformamide and 20 mL of dry tetrahydrofuran. To this mixture was added triethylamine (6.4 mL, 46.1 mmole). After 16 hours, the reaction mixture was diluted with 600 mL of ethyl acetate and washed with 150 mL each of water, 1 M aqueous hydrochloric acid, water, saturated aqueous sodium bicarbonate and brine.
  • Example 40 The compound of Example 40 (3.89 g, 6.49 mmole) was dissolved in 300 mL of methanol with stirring and purged with nitrogen. To this mixture was added 2 g of 10% palladium on carbon and stirred vigorously under 1 atmosphere of hydrogen. After 16 hours, the palladium was removed by filtration and the solvent removed in vacuo . The resulting oil was dissolved into 100 mL of ethyl acetate and the product was extracted into 150 mL saturated aqueous sodium bicarbonate. This was washed with 100 mL ethyl acetate and acidified using concentrated hydrochloric acid.
  • Example 4 The compound of Example 4 (1.50 g, 5.59 mmole) and the compound of Example 41 (1.9 g, 3.72 mmole) were dissolved with stirring in 10 mL of dry dimethylformamide and 20 mL of dry tetrahydrofuran. To this mixture was added 1-hydroxybenzotriazole monohydrate (0.76 g, 5.6 mmole) and 2- (lH-benzotriazol-1-yl) -1, 1,3, 3- tetramethyluronium hexafluorophosphate (2.12 g, 5.6 mmole) followed by N-methylmorpholine (2 mL, 19 mmole) .
  • Example 43 The compound of Example 43 (1 g, 1.6 mmole) was dissolved in 30 mL of 50:50 water:acetonitrile with stirring and cooled to 0°C in an ice water bath. To this solution was slowly added 50 mL of a 60 wt% solution of hexafluorophosphoric acid in water. After 1 hour, the pH of the reaction mixture was adjusted to about pH 4 using saturated aqueous sodium acetate. This mixture was filtered through a plug of Celite.
  • the title compound was obtained by purification from the filtrate by preparative HPLC (2 inch Vydak C18 column using a gradient consisting of 8 to 27% acetonitrile in water containing 0.1% trifluoroacetic acid run over 60 minutes at a flowrate of 115 mL/minute) and lyophilization of the pooled fractions.
  • Mass Spectroscopy (FAB) confirmed the calculated molecular weight of 635.7.
  • Carbonyldiimidazole (4.9 g, 30 mmole) was added to a suspension of benzylsulfonylmethionine sulfone (8.0 g, 28.5 mmole) in dichloromethane (60 mL) at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred an additional 17 hours. Methanol (2.3 mL, 57 mmole) was added and stirring was continued for 17 hours, at which time the reaction mixture was concentrated under vacuum.
  • Example 46 To a solution of the compound of Example 46 (5.0 g, 23.5 mmole) in tetrahydrofuran (40 mL) , was added over 15 minutes a 2M solution of trimethylaluminurn in toJucnc- (11.5 mL, 23 mmole) at 0°C After stirring for 1.3 hours benzylsulfonylmethionine sulfone methyl ester (1.4 g, 4.7 mmole) was added in tetrahydrofuran (10 mL) over 10 minutes. After the reaction mixture warmed to room temperature, it was stirred for 3 days.
  • Example 47 The compound of Example 47 (0.36 g, 0.76 mmole) was stirred with trifluoroacetic acid (5.0 mL) and dichloromethane (5.0 mL) at room temperature for 3.5 hours. Toluene (5.0 mL) was added and the reaction was concentrated under vacuum to give a residue.
  • Example 48 The compound of Example 48 (0.30 g, 0.47 mmole) was dissolved in methanol (12 mL) , acetic acid (2.0 mL) , and distilled water (2.0 L) and the solution was placed in a 250 mL Parr bottle. The reaction vessel was purged with argon and then 10% palladium on carbon catalyst (0.15 g) was added. The reaction mixture was shaken under hydrogen (45 psi) for 2.8 days, then was filtered through Celite and concentrated under vacuum to give crude title compound. This was purified by reverse phase HPLC on a two inch Vydak C-18 at 115 mL/minute, gradient 20-50% over 50 minutes and lyophilyzed to give the title compound as a white powder (0.2 g, 74%) .
  • Example 49 The compound of Example 49 (0.20 g, 0.34 mmole) was dissolved in acetonitrile (3.0 mL) , cooled to 0°C using an ice bath, and 6N hydrochloric acid (8.0 mL) was added. The ice-water bath was removed and the reaction was stirred at room temperature for 3 hours. An additional (1.0 mL) concentrated hydrochloric acid was added. After 1.0 hour, saturated sodium acetate (15 mL) was added. The solution was then filtered and then subjected to purification by HPLC.
  • Examples 45 through 50 are used to prepare other preferred compounds of the present invention which are shown below.
  • other substituted amines are used in the place of cyclohexylamine in Example 46.
  • other substituted amines would include cyclohexylmethylamine, 4- hydroxycyclohexylamine, aniline, benzylamine, cyclopentylamine, isopropylamine, isobutylamine, R and S sec-butylamine, 1-ethyl-l-aminopropane, n-butylamine, n- propylamine.
  • reaction mixture was stirred for 16 hours at room temperature, then was dissolved in 800 mL of ethyl acetate and washed with 200 mL each of water, 1 M aqueous hydrochloric acid, water, saturated aqueous sodium bicarbonate and brine. The organic phase was dried over anhydrous magnesium sulfate, filtered and the solvent removed in vacuo .
  • Rf 0.85 (1:9 methanol:dichloromethane) .
  • reaction mixture was dissolved in 600 mL of ethyl acetate and extracted in to saturated aqueous bicarbonate (2x200 mL) .
  • the combined aqueous fractions were washed with 200 mL ethyl acetate and then acidified to about pH 4 using hydrochloric acid. This was then extracted with ethyl acetate (2x300 mL) and the organic fractions were washed with 300 mL brine, dried over anhydrous magnesium sulfate, filtered and the solvent removed in vacuo to provide 6 g (55% yield) of the title compound as an off-white foam.
  • Rf 0.38 (1:9 methanol :dichloromethane) .
  • Example 4 The compound of Example 4 (1.18 g, 4.41 mmole) and the compound of Example 53 (2.1 g, 4.01 mmole) were dissolved with stirring in 10 mL of dry dimethylformamide and 30 mL of dry tetrahydrofuran. To this mixture was added 1-hydroxybenzotriazole monohydrate (0.81 g, 6.02 mmole) and 2-(lH-benzotriazol-1-yl)-1, 1,3, 3- tetramethyluronium hexafluorophosphate (2.28 g, 6.02 mmole) followed by N-methylmorpholine (2 mL, 20 mmole) .
  • reaction mixture was diluted with 700 mL ethyl acetate and extracted with 150 mL each of water, 1 M aqueous hydrochloric acid, water, saturated aqueous sodium bicarbonate and brine.
  • the organic phase was dried over anhydrous magnesium sulfate, filtered and the solvent removed in vacuo to afford 2.65 g (91% yield) of the title compound as an off-white foam.
  • the resulting oil was azeotroped with toluene to remove the remaining acetic acid to afford about 2 g of the title compound which was purified by HPLC on a two inch Vydak C-18 column at 115 mL/minute, gradient 20 to 60% over 50 minutes to give 0.7 g (30% yield) of the title compound as a white powder.
  • Example 55 The compound of Example 55 (0.7 g, 1.2 mmole) was dissolved in 20 mL of 50:50 water:acetonitrile with W .. intricateO..9.6./,1..9.4_.9.3-, PCT/US95/16866
  • Example 58 To a solution of the compound of Example 58 (3.33 g, 6.15 mmole) in 200 mL of methanol under a nitrogen blanket, was added 10% palladium on carbon (2.5 g) and the mixture was hydrogenated at 1 atmosphere for 4 days. The catalyst was removed and replaced 2 times during the course of the reaction. The mixture was then filtered and concentrated in vacuo to provide 2.0 g (72% yield) of the title compound as a white foam.
  • Example 4 The compound of Example 4 (1.54 g, 5.76 mmole) and the compound of Example 59 (2 g, 4.4 mmole) were dissolved with stirring in 10 mL of dry dimethylformamide and 10 mL of dry acetonitrile. To this mixture was added l-ethyl-3- (3-dimethylamino-propyl)carbodiimide hydrochloride salt (1.3 g, 6.6 mmole) and 1-hydroxybenzotriazole monohydrate (0.9 g, 6.6 mmole) followed by diisopropylethylamine (3 mL, 22 mmole) .
  • Example 61 The compound of Example 61 (1.9 g, 3.1 mmole) was dissolved in 30 mL of 50:50 water:acetonitrile with stirring and cooled to 0°C in an ice water bath. To this solution was slowly added 40 mL of a 60 wt% solution of hexafluorophosphoric acid in water. After 2 hours, the pH of the reaction mixture was adjusted to about pH 4 using saturated aqueous sodium acetate. This mixture was filtered through a plug of Celite.
  • the title compound was obtained by purification from the filtrate by preparative HPLC (2 inch Vydak C18 column using a gradient consisting of 8 to 25% acetonitrile in water containing 0.1% trifluoroacetic acid run over 55 minutes at a flowrate of 115 mL/minute) and lyophilization of the pooled fractions.
  • Mass spectroscopy (FAB) confirmed the theoretical molecular weight of 591.7.
  • Example 65 The title compound was prepared in the same manner as described in Example 59 using the compound of Example 63 (0.86 g, 1.78 mmole) in 100 mL of methanol and 0.5 g 10% palladium on carbon to provide 0.37 g (53% yield) of the title compound as a white foam.
  • Example 65 The title compound was prepared in the same manner as described in Example 59 using the compound of Example 63 (0.86 g, 1.78 mmole) in 100 mL of methanol and 0.5 g 10% palladium on carbon to provide 0.37 g (53% yield) of the title compound as a white foam.
  • the title compound was prepared in the same manner as described in Example 61 using 0.3 g of 10% palladium on carbon, 5 mL of water, 1 mL of glacial acetic acid, the compound of Example 65 (0.32 g, 0.53 mmole) in 30 mL of methanol to afford about 0.3 g (quantitative yield) of the title compound.
  • Example 62 The title compound was prepared in the same manner as in Example 62 using the compound of Example 66 (0.29 g, 0.5 mmole), 10 mL of 50:50 water:acetonitrile and 20 mL of a 60 wt% solution of hexafluorophosphoric acid in water. After 4 hours, the pH of the reaction mixture was adjusted to about pH 4 using saturated aqueous sodium acetate. This mixture was filtered thru a plug of Celite.
  • reaction mixture was dissolved in 1000 mL of ethyl acetate and washed with 300 mL each of water, 1 M aqueous hydrochloric acid, water, saturated aqueous sodium bicarbonate and brine.
  • the organic phase was dried over anhydrous magnesium sulfate, filtered and the solvent removed in vacuo.
  • Rf 0.78 (1:9 methanol : dichloromethane) .
  • Example 69 To a solution of the compound of Example 69 (13 g, 28.9 mmole) in 600 mL of methanol under a nitrogen blanket, was added 10% palladium on carbon (5 g) and the mixture was hydrogenated at 1 atmosphere for 16 hours. The mixture was then filtered and concentrated in vacuo to give an oil. The oil was dissolved in 400 L saturated aqueous sodium bicarbonate and washed with 300 mL ethyl acetate. The aqueous fraction was then acidified using hydrochloric acid to about pH 4 and extracted with ethyl acetate (2x500 mL) .
  • Example 4 The compound of Example 4 (2.37 g, 8.85 mmole) and the the compound of Example 70 (2.12 g, 5.90 mmole) were dissolved with stirring in 8 mL of dry dimethylformamide and 20 mL of dry acetonitrile. To this mixture was added l-hydroxy-7-azabenzotriazole (0.4 g, 2.95 mmole) and 0-(7- azabenzotriazol-1-yl) -1,1,3, 3-tetramethyluronium hexafluorophosphate (2.2 g, 5.9 mmole) followed by N- methylmorpholine (3 mL, 29.5 mmole) .
  • Example 72 The compound of Example 72 (0.57 g, 1.08 mmole) was dissolved in 20 mL of 50:50 water:acetonitrile with stirring and cooled to 0°C in an ice water bath. To this solution was slowly added 30 mL of a 60 wt% solution of hexafluorophosphoric acid in water. After 1 hour, the pH of the reaction mixture was adjusted to about pH 4 using saturated aqueous sodium acetate. This mixture was filtered thru a plug of Celite.
  • the title compound was obtained by purification from the filtrate by preparative HPLC (2 inch Vydak C18 column using a gradient consisting of 7-27% acetonitrile in water containing 0.1% trifluoroacetic acid run over 60 minutes at a flowrate of 115 mL/minute) and lyophilization of the pooled fractions.
  • Mass spectroscopy (FAB) confirmed the theoretical molecular weight of 500.6.
  • Example 74a To a solution of N-Boc-L-methionine sulfone sarcosine benzyl ester, Example 74a, (4.7 g, 10.0 mmole) in dry N,N- dimethyl formamide (20 mL) at 0°C, is added (2- iodoethyl)benzene (2.9 mL, 20.0 mmole) followed by sodium hydride (60% dispersion, 400 mg, 10.0 mmole) . The reaction is allowed to warm to room temperature and stirred for 24 hours.
  • the reaction is diluted with ethyl acetate (200 mL) and washed successively with saturated sodium bicarbonate (1x75 mL) , brine (1x75 mL) and 1 M aqueous hydrochloric acid (1x75 mL) .
  • the organic phase is dried over MgS ⁇ 4, filtered and the solvent removed in vacuo to provide the crude alkylated material .
  • This material is purified on a flush silica gel column to give purified material.
  • Example 76 To a solution of Example 74 (5.7 g, 10.0 mmole) in methanol (200 mL) is added 1.0 g 10% palladium on carbon and the reaction subjected to atmospheric hydrogenation for 24 hours. The reaction mixture is then filtered through a short plug of celite and the reaction volume reduced in vacuo to provide the desired acid.
  • Example 76 To a solution of Example 74 (5.7 g, 10.0 mmole) in methanol (200 mL) is added 1.0 g 10% palladium on carbon and the reaction subjected to atmospheric hydrogenation for 24 hours. The reaction mixture is then filtered through a short plug of celite and the reaction volume reduced in vacuo to provide the desired acid.
  • Example 76 To a solution of Example 74 (5.7 g, 10.0 mmole) in methanol (200 mL) is added 1.0 g 10% palladium on carbon and the reaction subjected to atmospheric hydrogenation for 24 hours. The reaction mixture is then filtered through a short plug of
  • Example 75 To a solution of Example 75 (4.8 g, 10.0 mmole) in N,N-dimethyl formamide (25 mL) , is added l-ethyl-3- (3- dimethyla inopropyl)carbodiimide hydrochloride salt (1.9 g, 10.0 mmole) followed by 1-hydroxybenzotriazole (2.3 g, 15.0 mmole) and the reaction stirred for 30 minutes. Then cyclic nitroarginine ethyl aminal hydrochloride (4.0 g, 15 mmole) is added followed by N-methyl morpholine (2.2 ml, 20.0 mmole) . The reaction is then stirred at room temperature for 18 hours.
  • reaction mixture is diluted with ethyl acetate (300 mL) and washed successively with saturated sodium bicarbonate (1x100 mL) , brine (1x100 mL) and 1 M aqueous hydrochloric acid (1x100 mL) .
  • the organic phase is dried over MgS04, filtered and the solvent removed in vacuo to provide the desired coupled product.
  • Example 76 To a solution of Example 76 (6.9 g, 10.0 mmole) in water (60 mL) , acetic acid (20 mL) and methanol (600 mL) in a 2000 mL Parr bottle is added 5.0 g of 10% palladium on carbon. The mixture is then shaken under a hydrogen atmosphere of 40 psi for 3 days. The catalyst is then removed by filtration and the filtrate concentrated in vacuo . The product is azeotroped with toluene to remove residual acetic acid to afford the title compound.
  • N-Boc-L-glutamic acid- (beta-acid) - benzyl ester 3.3 g, 10.0 mmole
  • N,N-dimethyl formamide 50 mL
  • 1-hydroxy-7-azabenzotriazole 2.0 g, 15.0 mmole
  • 0-(7-azabenzotriazol-l-yl)-1,1,3,3, - tetramethylaronium hexafluorophosphate (3.8 g, 10.0 mmole) and the reaction stirred at room temperature for 30 minutes.
  • Example 79 To a solution of Example 79 (4.5 g, 10.0 mmole) in dry ethyl acetate (100 mL) is added 4 M hydrochloric acid in dry dioxane (100 mL) at room temperature. The mixture is stirred for 3 hours and then evaporated in vacuo to provide the crude dihydrochloride salt. This compound is then dissolved in dry N,N-dimethyl formamide (50 mL) and benzylsulfonyl chloride (2.1 g, 12.0 mmole) is added followed by triethylamine (7.0 mL, 50.0 mmole).
  • reaction mixture is stirred at room temperature for 15 hours and then the reaction is diluted with ethyl acetate (400 mL) .
  • the organic phase is washed successively with saturated aqueous sodium bicarbonate (2x100 mL) , water (2x100 mL) then brine (2x100 mL) .
  • the organic phase is dried over MgS04, filtered and the solvent removed in vacuo to provide the title compound.
  • Example 80 To a solution of Example 80 (4.0 g, 10.0 mmole) in methanol (200 mL) is added 1.0 g of 10% palladium on carbon and the suspension subjected to atmospheric hydrogenation for 10 hours. The reaction mixture is filtered through a short plug of celite and the solvent is removed in vacuo to provide the title compound as the free acid.
  • Example 81 To a solution of Example 81 (3.1 g, 10.0 mmole) in N,N-dimethyl form-amide (40 L) is added l-ethyl-3- (3- di ethylaminopropyl)carbodiimide hydrochloride salt (2.1 g, 11.0 mmole) and 1-hydroxybenzotriazole (2.3 g, 15.0 mmole) . The mixture is stirred for 15 minutes at room temperature, then sarcosine benzyl ester p- toluenesulfonate salt (3.5 g, 10.0 mmole) is added, followed by N-methyl morpholine (2.2 mL, 20.0 mmole) .
  • reaction is stirred for 15 hours, then diluted with ethyl acetate (300 mL) and washed successively with saturated sodium bicarbonate (2x100 mL) , water (2x100 mL) and brine (2x100 mL) .
  • the organic phase is dried over MgS ⁇ 4, filtered and the solvent removed in vacuo to provide the title compound.
  • Example 82 To a solution of Example 82 (4.7 g, 10.0 mmole) in methanol (200 mL) is added 1.0 g 10% palladium on carbon and the reaction is subjected to atmospheric hydrogenation for 12 hours. The reaction is filtered through a short plug of celite and the solvent is removed in vacuo to provide the desired title compound.
  • Example 83 The compound of Example 83 (3.8 g, 10.0 mmole) is dissolved in N.N-dimethyl formamide (30 mL) and 2-(lH- benzotriazol-1-yl) -1,1,3,3-tetramethyluronium hexafluorophosphate (4.2 g, 11.0 mmole) is added, followed by 1-hydroxybenzotriazole monohydrate (2.3 g, 15.0 mmole) .
  • reaction mixture is stirred for 15 minutes, then the cyclic nitro arginine ethyl aminal hydrochloride salt (3.7 g, 10.0 mmole) is added, followed by N-methyl morpholine (2.2 mL, 20.0 mmole) and the reaction mixture stirred for 12 hours.
  • the reaction mixture is diluted with ethyl acetate (300 mL) and washed successively with saturated sodium bicarbonate (2x100 mL) , water (2x100 mL) and brine (2x100 mL) .
  • the organic phase is dried over MgS04, filtered and the solvent removed in vacuo to provide the crude title compound.
  • Example 86 To a solution of Example 84 (7.0 g, 10.0 mmole) in acetonitrile (25 mL) is added allyl iodide (1.8 mL, 20.0 mmole) and the reaction stirred at room temperature for 15 hours. The reaction is diluted with ethyl ether (250 mL) and the precipitate obtained is filtered off and dried in vacuo to provide the title compound.
  • Example 85 To a solution of Example 85 (8.6 g, 10.0 mmole) in water (60 mL) , acetic acid (20 mL) and methanol (600 mL) in a 2000 mL Parr bottle is added 5.0 g of 10% palladium on carbon. The mixture is then shaken under a hydrogen atmosphere of 40 psi for 3 days. The catalyst is then removed by filtration and the filtrate concentrated in vacuo . The product is azeotroped with toluene to remove residual acetic acid to afford the title compound.
  • Example 88 The compound of Example 86 (8.1 g, 10.0 mmole) is dissolved in 200 mL of 1:1 water:acetonitrile with stirring and cooled to 0°C in an ice water bath. To this solution is slowly added 300 mL of a 60 wt
  • Example 87 To a solution of Example 87 (6.9 g, 10.0 mmole) in water (60 mL) , acetic acid (20 mL) and methanol (600 mL) in a 2000 mL Parr bottle, is added 5.0 g of 10% palladium on carbon. The mixture is then shaken under a hydrogen atmosphere of 40 psi for 3 days. The catalyst is then removed by filtration and the filtrate concentrated in vacuo. The product is azeotroped with toluene to remove residual acetic acid to afford the title compound.
  • a solution of the compound of Example 39 (5.0 g, 9.7 mmole) in 100 mL of sieve dried ethyl acetate is prepared. To this, 26 mL of 5.7 M anhydrous hydrochloric acid/ethyl acetate (that is generated in si tu from acetyl chloride and dry methanol) is added dropwise. This mixture is stirred at room temperature for 8 hours until all starting material is consumed. The mixture is evaporated in vacuo and then azeotroped with toluene (3x50 mL) .
  • the resulting oil is suspended in acetonitrile (35 mL) , cooled to ice bath temperature, then benzylsulfonyl chloride (2.1 g, 11.1 mmole) and pyridine (2.9 g, 37.1 mmole) are added.
  • the reaction is removed from the ice bath after 30 minutes and allowed to stir at room temperature for 18 hours.
  • the reaction mixture is reduced in volume in vacuo .
  • the oil is taken up in 200 mL ethyl acetate and washed successively with 1 N hydrochloric acid (1x50 mL) , saturated sodium bicarbonate (1x50 mL) and brine (1x50 mL) . After drying with MgS04, the organic phase is reduced in vacuo to provide the desired sulfonamide product.
  • Example 92 To a solution of the compound of Example 90 (5.5 g, 10.0 mmole) in dichloromethane (100 mL) , is added trifluoroacetic acid (100 mL) and the mixture is stirred at room temperature for 4 hours, at which time starting material is consumed. The mixture is evaporated in vacuo to provide the desired acid.
  • Example 92 To a solution of the compound of Example 90 (5.5 g, 10.0 mmole) in dichloromethane (100 mL) , is added trifluoroacetic acid (100 mL) and the mixture is stirred at room temperature for 4 hours, at which time starting material is consumed. The mixture is evaporated in vacuo to provide the desired acid.
  • Example 92 To a solution of the compound of Example 90 (5.5 g, 10.0 mmole) in dichloromethane (100 mL) , is added trifluoroacetic acid (100 mL) and the mixture is stirred at room temperature for 4 hours, at which time
  • Example 91 To a solution of the compound of Example 91 (4.94 g, 10.0 mmole) in N,N-dimethyl formamide (40 mL) , is added 1- ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride salt (2.1 g, 11.0 mmole) and 1-hydroxybenzotriazole monohydrate (2.3 g, 15.0 mmole). The mixture is stirred for 15 minutes then (R)-alpha methyl benzyl amine (2.5 mL, 20.0 mmole) is added and the reaction stirred at room temperature for 12 hours.
  • 1- ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride salt 2.1 g, 11.0 mmole
  • 1-hydroxybenzotriazole monohydrate 2.3 g, 15.0 mmole
  • reaction mixture is diluted with ethyl acetate (300 mL) and washed successively with saturated sodium bicarbonate (1x75 mL) , brine (1x75 mL) and 1 M hydrochloric acid (1x75 mL) .
  • the organic phase is dried over MgS04, filtered and the volume reduced in vacuo to provide the desired carboxyamide.
  • Example 92 To a solution of the compound of Example 92 (5.97 g, 10.0 mmole) in methanol (100 mL) , is added 1.0 g of 10% palladium on carbon and the mixture is stirred under hydrogen gas at atmospheric pressure for 24 hours. The mixture is then filtered through a short plug of celite and the volume is reduced in vacuo to provide the corresponding acid.
  • cyclic nitroarginine ethyl aminal hydrochloride (4.0 g, 15.0 mmole) is added, followed by N-methyl morpholine (2.2 ml, 20.0 mmole) .
  • the reaction mixture is then stirred at room temperature for 18 hours.
  • the reaction mixture is diluted with ethyl acetate (300 mL) and washed successively with saturated sodium bicarbonate (1x100 mL) , brine (1x100 mL) and 1 M aqueous hydrochloric acid (1x100 mL) .
  • the organic phase is dried over MgS04, filtered and the solvent removed in vacuo to provide the desired coupled product.
  • Example 94 To a solution of Example 94 (8.6 g, 10.0 mmole) in water (60 mL) , acetic acid (20 mL) and methanol (600 mL) in a 2000 mL Parr bottle, is added 5.0 g of 10% palladium on carbon. The mixture is then shaken under a hydrogen atmosphere of 40 psi for 3 days. The catalyst is then removed by filtration and the filtrate concentrated in vacuo . The product is azeotroped with toluene to remove residual acetic acid to afford the title compound.
  • a mixture of thiouronium salt (63 g, Example 99A) in 1650 mL of water was mechanically stirred at ambient temperature for 30 minutes, cooled to 0° C and stirred for an additional 30 minutes.
  • the mixture was filtered through a 0.2 micron nylon filter, keeping the mixture at 0° C throughout. (The filtered precipitate contained the dichloromethyl- and methyl methoxycarbonyl benzenes, byproduct and starting material from the preparation of the compound of Example 98) .
  • the aqueous filtrate was cooled to 0°C, and with mechanical stirring chlorine gas was bubbled through the solution. After the green color persisted, nitrogen was passed through the mixture to remove the excess chlorine, then 700 mL of ether was added and the layers were separated.
  • Example 102 To the compound of Example 102 (3.16 g ⁇ 80%, 2.53 g, 0.010 mole) was added PCI5 (2.08 g, 0.01 mole) and the resultant slurry was stirred and cooled in an ice bath while POCI3 (11.50 g, 0.75 mole, 7.0 mL) was added slowly.
  • Example 99 The above-identified compound is prepared by coupling the product of either Example 99 or 103 with the product of Example 12 following procedures similar to those set forth in Example 13.
  • Example 105 To the compound of Example 105 (0.5 g, 0.83 mmole) in 100 mL of 4:1 ethanol:acetic acid, was added 0.5 g moist Pearlman's catalyst. The mixture was then stirred vigorously under hydrogen at atmosphere pressure for 16 hours. The catalyst was then removed by filtration and the filtrate concentrated in vacuo to afford about 0.45 g (quantitative yield) of the title compound.
  • Example 106 The compound of Example 106 was hydrolyzed according to the procedure in Example 21 to afford the title compound.
  • Example 19 The Compound in Example 19 (0.6 g, 0.85 mmole) was dissolved in 40 mL of ethanol. To this was added 4 mL of 1M sodium hydroxide solution. After 4 hours, 60 mL of 4:1 ethanol:acetic acid was added, followed by 0.5 g of moist Pearlman's catalyst and the mixture was stirred under hydrogen at atmospheric pressure for 16 hours. The catalyst was then removed by filtration and the filtrate concentrated in vacuo . The mass spectrum showed both the title compound and some of the 2- carboethoxybenzenesulfonamide. The resulting oil was again dissolved in 20 ml of 50:50 water and acetonitrile with stirring. To this was added 2 mL of 1M sodium hydroxide solution. After 2 days, HPLC showed only one peak. The pH was neutralized using 1M HCl and the reaction mixture was concentrated in vacuo to give the above-identified product.
  • Example 105 The title compound was synthesized according to the procedures in Examples 105 to 108 using (3- carbomethoxy)benzylsulfonyl-D-N ⁇ -N ⁇ 2-arginine sarcosine as a starting material in the procedure of Example 105.
  • Example 105 The title compound was synthesized according to the procedures in Examples 105 to 108 using (3- carboxy)benzylsulfonyl-D-N ⁇ -N02-arginine sarcosine as a starting material in the procedure of Example 105.
  • Example 37 wherein methylchloroformate is substituted for di-t-butyl dicarbonate and D-leucine (R 2 reagent) is substituted for the compound of Example 36, such that these starting materials produce methoxycarbonyl-D-leucine for R ⁇ X-NH-CH(R 2 )COOH, the following compounds were made.
  • the resulting compounds had the same stereochemical configuration at R as noted for the R 2 reagent.
  • Example 124 To a solution of the compound of Example 124 (4.4 g) in chloroform (100 mL) was added 50% m-chloroperbenzoic acid (13.8 g) . The solution was refluxed for 19 hours, then an additional portion of 50% m-chloroperbenzoic acid (6.9 g) was added and reflux was continued for 24 hours. The reaction mixture was cooled to room temperature and the solvent was removed under reduced pressure. The residue was diluted with ethyl acetate (150 mL) , washed with sodium bicarbonate solution (100 L) , sodium thiosulfate solution (100 mL) , and brine (100 mL) , then dried over magnesium sulfate and concentrated.
  • Example 127 The ester of Example 127 (3.4 g) was deblocked using piperidine as described in Example 18; the resulting carboxylic acid was coupled to N ⁇ -N ⁇ 2arginine ethyl aminal hydrochloride salt as described in Example 19; and hydrogenolysis of the nitro groups as described in Example 20 gave the title compound (1.8 g) in 59% overall yield.
  • Example 128 The compound of Example 128 (1.0 g) was hydrolyzed as described in Example 21 to give the title compound (0.54 g)
  • Example 130 The compound of Example 130 (1.0 g) was hydrolyzed as described in Example 21 to give the title compound (0.39 g ) .
  • Example 132 To the compound of Example 132 (50.Og), 200 mL of a 4M solution of HCl in dioxine was added. The solid eventually dissolved over 2 hours and showed no starting material by thin layer chromatography. The solution was then reduced in volume under vacuum and the resulting solid was washed with diethyl ether to provide 55.0 g (100%) of the title compound as a white solid.
  • Example 134 To a solution of the compound of Example 134 (1.6 g) in CH3OH (50 mL) , 250 mg 10% palladium on carbon is added. This mixture is then subjected to atmospheric hydrogenation at room temperature for 12 hours. The reaction mixture is then filtered through a pad of celite and the solvent is evaporated under vacuum to provide the title compound.
  • the ability of the compounds of the present invention to act as inhibitors of factor Xa catalytic activity was assessed by determining the concentration which inhibited enzyme activity by 50%, (IC50), using the purified human factor Xa.
  • the buffer used for all assays was HBSA (10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1% bovine serum albumin) .
  • the assay was conducted by combining in appropriate wells of a Corning microtiter plate, 50 microliters of HBSA, 50 microliters of the test compound diluted in HBSA (or HBSA alone for uninhibited velocity measurement) , and 50 microliters of the enzyme diluted in HBSA (prepared from purified human factor X obtained from Enzyme Research Laboratories according to the method described by Bock, P.E. et al. (1989) Archives of Biochem. Biophys. 273 : 375. The enzyme was diluted into HBSA prior to the assay in which the final concentration was 0.5 nM) .
  • the substrate S2765 N-alpha-benzyloxycarbonyl-D- argininyl-L-glycyl-L-arginine-p-nitroanilide dihydrochloride, obtained from Kabi Diagnostica and made up in deionized water followed by dilution in HBSA prior to the assay
  • the initial velocity of chromogenic substrate hydrolysis was measured by the change in absorbance at 405nm using a Thermo Max® Kinetic Microplate Reader over a 5 minute period in which less than 5% of the added substrate was utilized.
  • IC50 value 50% decrease in the initial rate of hydrolysis was defined as the IC50 value.
  • Table 1 shows the IC50 (nM) for certain preferred compounds (described in the noted Example) of the present invention.
  • Example B In vitro enzvme Assays for specificity determination .
  • the buffer used for all assays was HBSA (10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1% bovine serum albumin) .
  • the assay for IC50 determinations was conducted by combining in appropriate wells of a Corning microtiter plate, 50 microliters of HBSA, 50 microliters of the test compound at a specified concentration (covering a broad concentration range) diluted in HBSA (or HBSA alone for Vo (uninhibited velocity) measurement) , and 50 microliters of the enzyme diluted in HBSA. Following a 30 minute incubation at ambient temperature, 50 microliters of the substrate at the concentrations specified below, was added to the wells yielding a final total volume of 200 microliters.
  • the initial velocity of chromogenic substrate hydrolysis was measured by the change in absorbance at 405nm using a Thermo Max® Kinetic Microplate Reader over a 5 minute period in which less than 5% of the added substrate was utilized.
  • the concentration of added inhibitor which caused a 50% decrease in the initial rate of hydrolysis was defined as the IC50 value.
  • the factor Xa assay was conducted according to the procedure in Example A.
  • Thrombin Assay Thrombin catalytic activity was determined using the chromogenic substrate Pefachrome t-PA (CH3SO2-D- hexahydrotyrosine-glycyl-L-arginine-p-nitroaniline, obtained from Pentapharm Ltd.) .
  • the substrate was made up in deionized water followed by dilution in HBSA prior to the assay in which the final concentration was 300 micromolar (about 5-times Km) .
  • Purified human alpha- thrombin was obtained from Enzyme Research Laboratories, Inc. The enzyme was diluted into HBSA prior to the assay in which the final concentration was 0.25 nM.
  • rt-PA tissue plasminooen activator
  • rt-PA catalytic activity was determined using the substrate, Pefachrome t-PA (CH3S02-D-hexahydrotyrosine- glycyl-L-arginine-p-nitroaniline, obtained from Pentapharm Ltd.) .
  • the substrate was made up in deionized water followed by dilution in HBSA prior to the assay in which the final concentration was 500 micromolar (about 3-times
  • Human rt-PA (Activase ® ) was obtained from Genentech Inc. The enzyme was reconstituted in deionized water and diluted into HBSA prior to the assay in which the final concentration was 1.0 nM.
  • Plasmin catalytic activity was determined using the chromogenic substrate, S-2251 [D-valyl-L-leucyl-L-lysine-p- nirtoanilide dihydrochloride] , which was obtained from Kabi Diagnostica.
  • the substrate was made up in deionized water followed by dilution in HBSA prior to the assay in which the final concentration was 300 micromolar (about 2.5-times Km) .
  • Purified human plasmin was obtained from Enzyme Research Laboratories, Inc. The enzyme was diluted into HBSA prior to assay in which the final concentration was 1.0 nM.
  • Activated Protein C aPC catalytic activity was determined using the chromogenic substrate, Pefachrome PC (delta-carbobenzyloxy- D-lysine-L-prolyl-L-arginine-p-nitroaniline dihydrochloride) , obtained from Pentapharm Ltd. ) .
  • the substrate was made up in deionized water followed by dilution in HBSA prior to the assay in which the final concentration was 250 micromolar (about 3-times Km) .
  • Purified human aPC was obtained from Hematologic Technologies, Inc. The enzyme was diluted into HBSA prior to assay in which the final concentration was 1.0 nM.
  • Chymotrypsin catalytic activity was determined using the chromogenic substrate, S-2586 (methoxy-succinyl-L- arginine-L-prolyl-L-tyrosyl-p-nitroanilide) , which was obtained from Kabi Diagnostica.
  • the substrate was made up in deionized water followed by dilution in HBSA prior to the assay in which the final concentration was 100 micromolar (about 9-times Km) .
  • Purified (3X-crystallized; CDI) bovine pancreatic alpha-chymotrypsin was obtained from Worthington Biochemical Corp.
  • the enzyme was reconstituted in deionized water and diluted into HBSA prior to assay in which the final concentration was 1.0 nM.
  • Trypsin catalytic activity was determined using the chromogenic substrate, S-2222 (benzoyl-L-isoleucine-L- glutamic acid [gamma-methyl ester] -L-arginine-p- nitroanilide) , which was obtained from Kabi Diagnostica.
  • the substrate was made up in deionized water followed by dilution in HBSA prior to the assay in which the final concentration was 250 micromolar (about 4-times Km) .
  • Purified (3X-crystallized;TRL3) bovine pancreatic trypsin was obtained from Worthington Biochemical Corp. The enzyme was reconstituted in deionized water and diluted into HBSA prior to assay in which the final concentration was 0.5 nM.
  • IC50 values (nM) for certain compounds according to this invention are shown in Table 2, and demonstrate the selectivity of compounds of this invention for Factor Xa compared to related serine proteases .
  • Table 2 The IC50 values (nM) for certain compounds according to this invention are shown in Table 2, and demonstrate the selectivity of compounds of this invention for Factor Xa compared to related serine proteases .
  • Example C Ex vivo anticoagulant effects of (D) -camphorsulfonyl aspartvl sarcosine arginine aldehyde in human plasma.
  • ex vivo anticoagulant effect of a compound of the present invention was determined by measuring the prolongation of the activated partial thromboplastin time (APTT) and prothrombin time (PT) over a broad concentration range of the added inhibitor, using pooled normal human plasma.
  • APTT activated partial thromboplastin time
  • PT prothrombin time
  • Fresh frozen citrated pooled normal human plasma was obtained from George King Biomedical, Overland Park, KA.
  • the measurements for the APTT and PT were made using the Coag-A-Mate RA4 automated coagulometer (General Diagnostics, Organon Technica, Oklahoma City, OK) using
  • the assays were conducted by making a series of dilutions of the test compound in rapidly thawed plasma followed by adding either 200 microliters or 100 microliters to the wells of the assay carousel for the APTT and PT measurement, respectively.
  • Example D Multiple Extracorporeal Shunt Model in Rats Utilizing Oral Dosing
  • the compound of Example 56 was evaluated in a multichamber A-V shunt model in rats.
  • the A-V shunt model is one of the most common and generally used systems to evaluate antithrombotic compounds. Smith, J.R. and White, A.M. Br. J. Pharmacol., 77: 29-38 (1982) . In this model a localized clot made up of primarily fibrin with some platelet and macrophage involvement (Shand, R. A. and Smith, J.R. and Wallis, R. B. Thromb. Res., 26: 223-232
  • thrombogenic surface typically a segment of silk or cotton thread
  • sialstic chamber which is part of an exteriorized shunt between the carotid artery and jugular vein.
  • the procedure described in this Example is a modified A-V shunt model that allows for oral dosing of test agents and subsequent evaluation of efficacy over a two to three hour window in time.
  • mice Male Harlan Sprague Dawley rats (420-450 g) were acclimated at least 72 hours prior to use. The animals were fasted for 12 hours prior to surgery with free access to water. Unanesthetized animals were grouped into four dosage groups (six or seven animals per group) and administered test agents orally via gavage needle, at doses of 1.0, 3.0, 10 and 50 mg/kg. Immediately after oral dosing, animals were anesthetized with sodium pentobarbital (Nembutal) given intraperitoneally at a dose of 50 mg/kg body weight, and placed on a isothermal pad to maintain body temperature.
  • sodium pentobarbital Naembutal
  • the level of anesthesia was monitored every 15 minutes by neuro-response to a tail pinch, respiration and core temperature.
  • the desired depth of surgical anesthesia was maintained by administering subsequent doses (5 mg/kg) intravenously.
  • the left femoral artery was catheterized using standard procedures for blood pressure monitoring and blood sampling, with polyethylene tubing (PE50) .
  • PE50 polyethylene tubing
  • the left femoral vein was catheterized with PE50 tubing for delivery of anethestic.
  • the exteriorized shunts were assembled by connecting two pieces of saline filled 12.5 cm PE90 tubing with a 6 6/19493
  • This procedure takes place at predetermined intervals (60, 90, 120, and 150 minutes after oral dosing) to allow assessment of efficacy over a large window in time.
  • Four shunts were placed with flow initiated at 45, 75, 105, and 135 minutes after oral compound administration. Clot weight from the four shunts was the primary endpoint of the protocol. Blood pressure, heart rate core temperature and respiration were monitored continuously. Following termination of the experiment the animal was euthanized with a 120 mg/kg dose of Nembutal. One experiment was performed per animal.
  • Table 3 presents the data from this procedure, and demonstrates the oral activity of the compound of Example 56 in inhibiting thrombosis. At every dose tested, animals treated with the compound of Example 56 demonstrated smaller clots than animals treated with water. The compound's ability to decrease clot formation was maintained over time.
  • the antithrombotic (prevention of thrombus formation) properties of compounds according to this invention were evaluated using an established experimental rat model of acute vascular thrombosis.
  • the rat FeCl3 model is a well characterized model of platelet dependent, arterial thrombosis which has been used to evaluate potential antithrombotic compounds.
  • Kurz, K. D. , Main, B. W. , and Sandusky, G. E., Thromb. Res., 60 : 269-280 (1990) .
  • a platelet-rich, occlusive thrombus is formed in a segment of the rat carotid artery treated locally with a fresh solution of FeCl3 absorbed to a piece of filter paper.
  • the FeCl3 is thought to diffuse into the treated segment of artery and cause de- endothelialization of the affected vessel surface. This results in the exposure of blood to subendothelial structures which in turn cause platelet adherence, thrombin formation and platelet aggregation. The net result is occlusive thrombus formation.
  • the effect of a test compound on the incidence of occlusive thrombus formation following application of FeCl3 is monitored by ultrasonic flowtometry and is used as the primary end point.
  • the use of flowtometry to measure carotid artery blood flow is a modification of the original procedure in which thermal detection of clot formation was employed.
  • Kurz, K. D., Main, B. W. and Sandusky, G. E., Thromb. Res. , £0_: 269-280 (1990) .
  • mice Male Harlan Sprague Dawley rats (420-450 g) were acclimated at least 72 hours prior to use and fasted for 12 hours prior to surgery with free access to water.
  • the animals were prepared, anesthetized with Nembutal followed by the insertion of catheters for blood pressure monitoring, drug and anesthesia delivery.
  • the left carotid artery was isolated by making a midline cervical incision followed by blunt dissection and spreading techniques to separate a 2 cm segment of the vessel from the carotid sheath.
  • a silk suture is inserted under the proximal and distal ends of the isolated vessel to provide clearance for the placement of a ultrasonic flow probe (Transonic) around the proximal end of the vessel. The probe is then secured with a stationary arm.
  • Transonic ultrasonic flow probe
  • test compound was administered as a single intravenous bolus at various doses after placement of the flow probe and 5 min prior to the thrombogenic stimulus.
  • a 3mm diameter piece of filter paper (Whatman #3) soaked with 10 ⁇ L of a 35% solution of fresh FeCl3 (made up in water) was applied to the segment of isolated carotid artery distal to the flow probe. Blood pressure, blood flow, heart rate, and respiration were monitored for 60 minutes. The incidence of occlusion (defined as the attainment of zero blood flow) was recorded as the primary end point.
  • a ED50 is defined as the dose that prevents the incidence of complete thrombotic occlusion in 50% of animals tested
EP95944234A 1994-12-21 1995-12-21 N-SUBSTITUTED GLYCINE DERIVATIVES AS INHIBITORS OF FACTOR Xa Withdrawn EP0801654A1 (en)

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US484509 1983-04-13
US08/361,794 US5696231A (en) 1994-12-21 1994-12-21 N-substituted glycine derivatives as enzyme inhibitors
US361794 1994-12-21
US08/484,509 US6025472A (en) 1994-12-21 1995-06-07 N-substituted glycine derivatives as enzyme inhibitors
PCT/US1995/016866 WO1996019493A1 (en) 1994-12-21 1995-12-21 N-SUBSTITUTED GLYCINE DERIVATIVES AS INHIBITORS OF FACTOR Xa

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MX9704684A (es) 1998-02-28
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WO1996019493A1 (en) 1996-06-27
AU4608696A (en) 1996-07-10
HUT77524A (hu) 1998-05-28
AU716995B2 (en) 2000-03-16
US6025472A (en) 2000-02-15

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