EP0655133A1 - Dispositif de detection d'amines volatiles - Google Patents

Dispositif de detection d'amines volatiles

Info

Publication number
EP0655133A1
EP0655133A1 EP94908062A EP94908062A EP0655133A1 EP 0655133 A1 EP0655133 A1 EP 0655133A1 EP 94908062 A EP94908062 A EP 94908062A EP 94908062 A EP94908062 A EP 94908062A EP 0655133 A1 EP0655133 A1 EP 0655133A1
Authority
EP
European Patent Office
Prior art keywords
sample
swab
indicator
test composition
volatile amines
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP94908062A
Other languages
German (de)
English (en)
Inventor
John Anthony Bolbot
Eric Joseph D'costa
Christopher Sonnex
Duncan Stewart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge Research and Innovation Ltd
Original Assignee
Cambridge Research and Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Research and Innovation Ltd filed Critical Cambridge Research and Innovation Ltd
Publication of EP0655133A1 publication Critical patent/EP0655133A1/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/40—Detection of gases
    • C12Q2304/48—Ammonia or volatile amines
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N2001/028—Sampling from a surface, swabbing, vaporising

Definitions

  • the present invention relates to a diagnostic apparatus and method suitable for the detection of volatile amines.
  • BV bacterial vaginosis
  • volatile amines particularly trimethyl amines which, when released, give a strong fishy odour.
  • One test which is currently available to identify these volatile amines is to put some vaginal fluid on a slide with normal saline solution and then add potassium hydroxide. The hydroxide drives off the volatile amine and a strong fishy odour is released.
  • a diagnostic device for carrying out a simple test of a similar nature is described in GB 2199944. This device comprises a test tube containing an alkaline medium and a swab.
  • the swab is placed in contact with vaginal fluid and then inserted into the alkaline medium in the test tube.
  • the presence of volatile amines is detected by smell through a window or opening in the side of the test tube. While this device has resulted in major improvements in the ease of diagnosis of BV, there is a major disadvantage of the technique in that the detection of the volatile amines is subjective, difficult to standardise and unpleasant.
  • the present invention seeks to provide a diagnostic device and method which does not suffer from these disadvantages.
  • an apparatus for detecting the presence of volatile amines in a sample comprising a receptacle containing a test composition, means for retaining the sample in contact with the test composition and indicator means arranged to detect vapour arising as a result of reaction of the sample with the test composition and adapted to change colour if said vapour contains volatile amines, the test composition comprising a substance for driving out of solution any volatile amines present in the sample.
  • the test composition also comprises a second substance for trapping any ammonia from the sample to avoid the ammonia making contact with the indicator means.
  • the present invention provides an apparatus for detecting the presence of volatile amines, important in diagnosing BV, without the requirement to carry out the rather unpleasant "sniff test" required with the prior art device.
  • the conversion of the detection of volatile amines from a test by smell to a test by colour change is not simple.
  • the first substance is an alkali.
  • ammonia is produced from ammonium salts naturally present in body fluids.
  • the nose is more sensitive to the vapour of amines than to ammonia.
  • most chemical indicators capable of detecting one will also detect the other so that false positives could arise.
  • the present inventors have thus provided in the preferred embodiment not only a substance to drive off the volatile amines for detection by the indicator means but also a substance to trap the ammonia.
  • the first and second substances are provided by an alkaline solution of dipotassium tetraiodomercurate.
  • the potassium tetraiodomercurate can be used as an ammonia trap since it reacts with ammonia to give a precipitate of complex ions. Thus, the ammonia is not driven off with the volatile amines so that the danger of false positives is avoided.
  • Dipotassium tetraiodomercurate can be used in a concentration of 65 grams per litre of solution.
  • the presence of volatile amines causes a change in pH.
  • a wide range of pH indicator dyes can be selected as the indicator substance.
  • the indicator substance or substances should be sensitive in the appropriate pH region to small changes in pH.
  • the colour change of the indicator should be detected easily by eye, even by users with visual impairments such as colour -blindness.
  • the indicator dye should of course have properties which render it suitable for incorporation into a manufactured device, for example it should be chemically stable.
  • One preferred indicator means comprises nitrazine yellow which changes its colour from yellow to blue in the presence of trimethyl amine and other amines at a pH of about 6.5. Nitrazine yellow is particularly sensitive at this pH and will change its colour with only a small change in pH (from 6.0 - 7.2) .
  • the apparatus can be used to carry out the test in an automated fashion.
  • a diagnostic device comprising a first part defining the receptacle containing the test composition; means for supporting a swab carrying the sample so that the sample is in contact with the test composition; and a second part capable of engagement with the first part and carrying the indicator means such that engagement of the second part with the first part closes off the receptacle and causes the swab to come into contact with the test composition, the indicator means being visible in use of the device.
  • the first part comprises a base having an upstanding wall defining said receptacle and a guide constituting the swab supporting means and the second part has a ledge for engaging said upstanding wall, an upper surface carrying the indicator means and a lower surface for applying pressure to the swab to bring it into contact with the test composition in the receptacle, there being at least one passageway for enabling vapour emitted during a test to pass from the lower surface to the upper surface and thereby to contact the indicator means.
  • the second part can comprise an indicator cap assembly having a cap body defining the ledge, an indicator pad constituting the indicator means said pad being arranged between the upper surface of the cap assembly and a mesh.
  • an indicator cap assembly having a cap body defining the ledge, an indicator pad constituting the indicator means said pad being arranged between the upper surface of the cap assembly and a mesh.
  • a diagnostic device comprising a body part containing the test composition in a manner such that the test composition can be caused to move relative to the body part by the insertion of a swab carrying the sample, and an indicator part containing the indicator means and arranged such that when the test composition has been moved by the swab carrying the sample relative to the body part, vapour released therefrom is brought into contact with the indicator means.
  • the swab is supported by a swab stick which carries a seal defining behind the swab and with the body part a reaction chamber.
  • the present invention also provides a method of carrying out a test for detecting the presence of volatile amines, the method comprising: taking a sample of body fluid from a patient. placing the sample in contact with a test composition comprising a first substance to drive volatile amines out of solution and a second substance to absorb ammonia, and detecting the colour change of an indicator means placed in contact with vapour driven off from the sample by the first substance.
  • the invention also provides a method of diagnosing bacterial vaginosis by carrying out the steps of the above defined method.
  • Figure 1 is a section through a base part of a first embodiment of a diagnostic device
  • Figure 2 is a section through an indicator cap assembley of a diagnostic device
  • Figure 3 is a view of ari indicator cap assembly from below
  • Figure 4 is a view of an indicator cap assembly from above
  • Figure 5 is a view from above of a diagnostic device with its swab in a test position
  • Figure 6 is a section taken along the lines VI-VI in Figure 5;
  • Figure 7 is a view of the indicator cap assembly along the line of the swab test position
  • Figure 8 is a view of the diagnostic device from above with the indicator cap assembly in place
  • Figure 9 is a section taken along the line IX-IX in Figure 8.
  • Figure 10 is a view of the diagnostic device with the swab in the test position viewed along the line of the swab stick test position;
  • Figure 11 is a view of the diagnostic device showing the appearance for a negative test;
  • Figure 12 is -a view of the diagnostic device showing the appearance for a positive test
  • Figure 13 is a longitudinal section through a second embodiment of the present invention.
  • Figure 14 illustrates-- a swab for use in the second embodiment of the present invention
  • FIGS 15a to 15c demonstrate the use of the second embodiment of the present invention.
  • Figure 16 is a sketch illustrating an apparatus used to carry out tests in accordance with the present invention.
  • Figure 17 is a section through a reaction tube forming part of an apparatus in accordance with another embodiment of the present invention.
  • Figure 18 is a section through a closure assembly for cooperation with the reaction tube of Figure 17;
  • FIGS 19a to 19f illustrate use of the embodiment of Figures 17 and 18.
  • a diagnostic device comprises a base part illustrated in Figure 1 and an indicator cap assembly illustrated in Figures 2 to 4.
  • the base part comprises a base support 2 having an upstanding wall 4 which defines a receptacle or reaction chamber 6.
  • the base part also has a further upstanding support 8 for supporting a swab stick 10, the upstanding support 8 and the wall 4 of the reaction chamber 6 each defining respective swab stick guide walls 12 and retaining slots 14.
  • the swab stick 10 carries a swab head 16 and the arrangement of the guide walls 12 and retaining slots 14 is such that when the swab stick 10 is supported thereby the swab head 16 lies within the reaction chamber 6.
  • the reaction chamber has an absorbent matrix 18 secured within the wall 4 of the reaction chamber 6 by an O-ring seal 20.
  • the absorbent matrix can take the form of a sponge or similar material and is impregnated with a chemical composition for carrying out a diagnostic test. This composition will be described in more detail hereinafter.
  • the indicator cap assembly comprises a body 22 having an upper annular ring 24 which has a lower surface defining a ledge 24a and - an •upper surface supporting a ring 26 of adhesive.
  • the adhesive secures a gas-permeable membrane 28 across an upper surface 29 of the body 22.
  • An indicator pad 30 is secured between the gas-permeable membrane 28 and a mesh 32 also secured by the adhesive 26.
  • the body 22 defines four centrally located passageways 34 (seen most clearly in Figure 3) extending from a lower surface 31 of the body 22 to the upper surface 29 for a purpose to be described hereinafter.
  • the body 22 also has a swab stick retaining lug 36.
  • Figure 4 shows the test cap indicator assembly from above illustrating the adhesive 26, the mesh 32 and the indicator pad 30 viewed through the mesh.
  • Figure 5 is a plan view of the base part with the swab in position for a test.
  • the swab stick 10 is supported in the slots 14 and between the guide walls 12 (see Figure 6).
  • the swab head 16 then sits in the surface of the absorbent matrix 18.
  • Figure 7 shows the indicator cap assembly when viewed along the swab stick 10. In particular, the swab retaining lug 36 can readily be seen.
  • Figure 8 to 10 show the diagnostic device in the process of a test.
  • Figure 9 is a section through the device showing the swab 10 supported by the retaining slots 14 with the swab head pushed into the absorbent matrix 18 by the indicator cap assembly.
  • the indicator cap assembly seals the reaction chamber 6 by virtue of the annular ledge 24a engaging the wall 4 of the reaction chamber 6.
  • the passages 34 allow the release of vapour resulting from the test to pass through the gas-permeable membrane 28 to the indicator pad 30.
  • the swab stick retaining lug 36 sits neatly in the guide walls 12 defining the slot 14.
  • the absorbent matrix 18 contains an alkaline solution of dipotassium tetraiodomercurate which provides a substance to drive off volatile amines, namely potassium hydroxide and a substance, potassium tetraiodomercurate, which traps and retains- any ammonia present in the sample to prevent it from causing the indicator to give a false positive.
  • the matrix 18 can comprise any absorbent material, such as gauze, impregnated with the chemicals in solution and then dried. The matrix 18 must thus be rehydrated before use.
  • the indicator pad 30 comprises an indicator selected to change colour around the pH range of a contaminated sample, namely
  • nitrazine yellow is used which changes colour from yellow to blue as a result of the change in-pH caused by the presence of trimethyl amine.
  • the device is supplied ready for use with the base part and indicator cap assembly secured to one another and with a separate sterile pack containing a swab.
  • the swab is used to take a sample, the indicator cap assembly is removed from the base part, and the matrix 18 is wetted with water to rehydrate it. The indicator pad is also wetted by applying water through the mesh.
  • the swab is then placed on the base part as shown in Figures 5 and 6.
  • the indicator cap assembly is then immediately replaced onto the base part and pressed into position to squash the swab head 16 into the reaction chamber
  • Figure 13 illustrates an indicator tube assembly in section, the assembly comprising a tube body 40 sealed at one end thereof by a cap 42 and at the other end thereof by an indicator assembly 44.
  • the indicator assembly takes the form of a closure device having a tubular part 46 for fitting over the open end of the tube body 40 and a conical part 48.
  • the conical part 48 houses a fixed plug 50 of a firm gel which contains the indicator chemicals.
  • a gas permeable membrane 52 is provided extending across the conical part 48.
  • the tube body 40 also houses a plug 54.of soft (but not runny) gel, movable along the tube body 40.
  • the plug 54 of gel in the tube body contains the test composition, namely a first substance for driving off the volatile amines and a second substance for trapping any ammonia released as a result of reaction of the first substance with body fluids.
  • the substances can be provided by a solution as in the first embodiment of the present invention.
  • the indicator chemicals in the plug 50 of gel in the conical part of the indicator assembly 44 can comprise nitrazine yellow.
  • the diagnostic device also comprises a swab assembly comprising a swab stick 56 carrying a swab head 58.
  • the swab stick also carries an O-ring piston seal 60 the purpose of which will be described hereinafter.
  • the piston seal 60 is located just behind the swab head 58.
  • the cap 42 is removed from the test tube body, the swab 58 is used to take a sample of body fluid and is then inserted into the open end of the tube body 40.
  • the swab is pushed to the left in Figure 15a until it enters the plug 54 containing the test composition.
  • the swab head is pushed into the plug and causes the plug to move with it towards the other end of the tube body.
  • the swab head immersed in the plug 54 eventually abuts the gas permeable membrane 52. Vapours released from the reaction of the components of the body fluid with the reagents in the test- composition pass through the gas permeable membrane and contact the indicator chemicals in the plug 50.
  • the gas permeable membrane allows the swab head to come close to the plug 50 without the test composition of the plug 54 affecting the indicator chemicals.
  • the plug 50 then turns colour to indicate a positive reaction or shows no change in colour if the test is negative.
  • the O-ring piston seal 60 protects the user from coming into contact with the alkali reagent in the plug 54.
  • the piston seal also ensures that the amine vapour is contained in a test chamber defined at the end of the tube body by the indicator assembly conical part and the piston ring seal. This allows for increased sensitivity and speed of the test. It also allows hygenic disposal of the assembly after use without release of the amine vapour and its associated smell.
  • the conical part can be provided at its tip with a lens to magnify the indicator gel 50 for ease of use.
  • test sample was placed onto a swab and the swab was then exposed to an ammonia trap or to a control solution (no ammonia trap) .
  • the test result was the time taken to change the colour of a pH indicator pad and this was recorded.
  • the swab illustrated in Figure 16 is the same as that in Figure 14 but is supplied initially without the seal 60. After use, the used swab is placed head up in a container ready for testing. The seal is pushed onto the swab handle up to the base of the swab and secured with a small drop of superglue. A drop (0.1ml) of wetting reagent was put on a petri dish lid. Fine (dry) tweezers were used to take an indicator disc and wet it. Excess wetting was removed by touching the disc on the surface of filter paper or the like.
  • the wetted disc was stuck on the inside wall of a syringe barrel 72 about 3mm inside the open end as shown in Figure 16 with the indicator disc being denoted by reference numeral 70.
  • the swab is inserted into the bottom end of the syringe barrel and pushed halfway up.
  • 0.05ml of solution with an ammonia trap or control solution is dispensed on top of the swab as indicated by arrow R in Figure 16.
  • the swab handle 56 was twisted between thumb and forefinger a couple of times (to help the solution penetrate the swab) whilst pushing the swab further up the barrel until its tip is near but not touching the indicator disc 70.
  • the open end 74 of the syringe barrel was then quickly sealed with a sealing disc 76.
  • test sample and the ammonia trap were added in 50ul volumes. Trimethylamine was used as the hydrochloride, ammonia was used as ammonium chloride.
  • the ammonia trap used was an alkaline solution of dipotassium tetraiodomercurate, 65 g/L. In the control experiments (no ammonia trap) a solution of 50 mM sodium hydroxide was used instead.
  • Figure 17 shows a reaction tube 81 which contains an absorbant pad 82 containing an alkali reagent and an ammonia trap as discussed above with reference to the first and second embodiments of the invention.
  • Reference numeral 83 denotes a cap for the reaction tube 81.
  • Figure 18 illustrates a closure and operating assembly for the reaction tube of Figure 17.
  • the assembly comprises a cylindrical piston body 86 having an upper annular part which engages the top of the reaction tube in use and which is denoted by reference numeral 86a.
  • the piston body 86 carries at its lower end two piston sealing rings 99.
  • passageway 100b extending centrally of the piston body through which a swab handle can pass in use as described later.
  • gas transfer channel 92 which extends along the piston body and which is surrounded at its upper end by a pointed annulus 93.
  • the closure and operating assembly also comprises a transparent cylindrical indicator cap 87.
  • the cap 87 has on its underside a locating ring 94 which engages a corresponding locating circular recess 95 in the upper face of the annular part 86a of the piston body 86.
  • the cap 87 defines an indicator solution chamber 96 which contains indicator solution illustrated by reference numeral 89.
  • the indicator solution chamber 96 has in its upper end a vent hole 88 sealed by a vent closure 88a. It has at its lower end a narrow capillary access port 97.
  • the access port 97 is sealed by a membrane 90.
  • a passageway 100a extends through the cap 87 in alignment with the passageway 100b of the piston body 86. These passageways 100a and 100b receive a swab handle 85 carrying a swab 84.
  • a sample is taken from a patient using the closure assembly as illustrated in Figure 18.
  • the cap 83 is then removed from the reaction tube 81 and the closure assembly with the patient sample is placed into the reaction tube 81 so as to fully immerse the swab 84 in the absorbant pad 82 and to simultaneously locate the piston body 86 into the reaction tube 81 as illustrated in Figure 19a.
  • the locating ring 94 of the cap 87 is engaged by the locating recess 95 of the piston body 86 in such a fashion as to leave a gap 91 between the upper face of the annular part of the piston body and the lower face of - the indicator cap 87. In this position, the pointed annulus 93 touches but does not penetrate the membrane 90 of the indicator solution chamber 96.
  • the indicator cap 87 is then snapped into the annular part 86a of the piston body 86 so that the pointed annulus 93 pierces the membrane 90 of the indicator solution chamber 96. This has the effect of connecting the gas transfer channel 92 within the piston body 86 to the narrow capillary access port 97 of the indicator cap 87. This is illustrated in Figure 19b. With the combined assembly still in a vertical position, the closure 88a for the vent 88 is removed. This is illustrated in Figure 19c.
  • a sealed reaction chamber is formed which leaves a headspace for amine gas to collect above the liquid reaction area.
  • the device has a space for the amine gases to enter.
  • the operation of the mechanism of the device has the effect of forcing the gas to where it is required (namely to bubble through the indicator solution) .
  • the gas in the headspace is forced into the gas transfer channel and passes through this so as to make good contact with a small volume of indicator fluid.

Abstract

L'invention concerne un dispositif servant à détecter la présence d'amines volatiles dans un spécimen. Le dispositif comporte un réceptacle (6, 40) contenant une composition d'analyse. Un batonnet ouaté (10, 16, 58) met le spécimen en contact avec la composition d'analyse et une substance indicatrice (30, 44) est conçue pour détecter la vapeur se dégageant sous l'effet de la réaction du spécimen avec la composition d'analyse, ainsi que pour changer de couleur si ladite vapeur contient des amines volatiles. On peut utiliser ce dispositif pour diagnostiquer des vaginites bactériennes.
EP94908062A 1992-08-14 1993-08-09 Dispositif de detection d'amines volatiles Ceased EP0655133A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB929217330A GB9217330D0 (en) 1992-08-14 1992-08-14 Diagnostic apparatus
GB9217330 1992-08-14
PCT/GB1993/001678 WO1994004916A1 (fr) 1992-08-14 1993-08-09 Dispositif de detection d'amines volatiles

Publications (1)

Publication Number Publication Date
EP0655133A1 true EP0655133A1 (fr) 1995-05-31

Family

ID=10720384

Family Applications (1)

Application Number Title Priority Date Filing Date
EP94908062A Ceased EP0655133A1 (fr) 1992-08-14 1993-08-09 Dispositif de detection d'amines volatiles

Country Status (4)

Country Link
EP (1) EP0655133A1 (fr)
AU (1) AU4723193A (fr)
GB (1) GB9217330D0 (fr)
WO (1) WO1994004916A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9315440D0 (en) * 1993-07-26 1993-09-08 Cambridge Res & Innovation An amine tester for diagnosing bacterial vaginosis and vaginitus
GB9717209D0 (en) * 1997-08-14 1997-10-22 Aromascan Plc Condition detector
US6244096B1 (en) * 1998-06-19 2001-06-12 California Institute Of Technology Trace level detection of analytes using artificial olfactometry
US6494833B1 (en) 2001-06-19 2002-12-17 Welch Allyn, Inc. Conditioning apparatus for a chemical sensing instrument
EP1419266B1 (fr) * 2001-08-17 2008-01-16 Osmetech PLC Detection de la vaginose bacterienne
DE102007006505B3 (de) * 2007-02-09 2008-08-07 Voll, Wolfgang, Dr. Behälter und medizinische Tupfer für biologische Materialien
US9700286B2 (en) * 2012-04-25 2017-07-11 Kph Diagnostics Inc. Fluid sample collection and testing device

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234044A (fr) * 1968-04-09 1971-06-03
DE1935766A1 (de) * 1969-07-14 1971-01-28 Dahl Dr Ludwig H Diagnostiziermittel zum Nachweis pathologisch veraenderter Vaginalflora
DD207579A1 (de) * 1982-04-12 1984-03-07 Emil Mehnert Verfahren zur quantitativen bestimmung des eiweiss-bzw. gesamtstickstoff-gehaltes in allen proteintraegern sowie des ammoniak-gehaltes in organischem material
AU573203B2 (en) * 1983-01-28 1988-06-02 Dairy and Foods Labs.Inc. Preservative composition with colour indicator
GB2199944B (en) 1987-01-05 1991-01-02 Christopher Sonnex An amine tester for diagnosing bacterial vaginosis and vaginitis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9404916A1 *

Also Published As

Publication number Publication date
WO1994004916A1 (fr) 1994-03-03
AU4723193A (en) 1994-03-15
GB9217330D0 (en) 1992-09-30

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