EP0584277B1 - Adaptateur pour tube de centrifugation - Google Patents

Adaptateur pour tube de centrifugation Download PDF

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Publication number
EP0584277B1
EP0584277B1 EP92913835A EP92913835A EP0584277B1 EP 0584277 B1 EP0584277 B1 EP 0584277B1 EP 92913835 A EP92913835 A EP 92913835A EP 92913835 A EP92913835 A EP 92913835A EP 0584277 B1 EP0584277 B1 EP 0584277B1
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EP
European Patent Office
Prior art keywords
segment
adapter
inboard
rotor
segments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP92913835A
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German (de)
English (en)
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EP0584277A1 (fr
EP0584277A4 (fr
Inventor
William Andrew Romanuaskas
Thomas Edward Sheeran, Jr.
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Sorvall Products LP
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Sorvall Products LP
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Publication of EP0584277A4 publication Critical patent/EP0584277A4/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B2005/0435—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with adapters for centrifuge tubes or bags

Definitions

  • the present invention relates to an adapter for holding a centrifuge tube in a centrifuge rotor cavity, and in particular, to an adapter having two segments, the segments being in some cases joined by a hinge, the hinge axis extending perpendicular to the axis of the adapter.
  • a device known as a tube adapter When the shape and size of a centrifuge tube does not closely conform to the shape and size of the rotor cavity in which it is to be disposed a device known as a tube adapter is usually employed.
  • the tube adapter has an interior cavity having a shape and size which closely conforms to the shape and size of the centrifuge tube being adapted.
  • the exterior shape and size of the adapter closely conforms to the shape and size of the rotor cavity in which the tube is to be used.
  • the adapter serves to support a tube within the cavity in which it is received and thus serves to prevent deformation of the tube during centrifugation.
  • an adapter formed of a single unitary member is the device disclosed in United States Patent 4,304,356 (Chulay et al.). This adapter supports only the neck region of the centrifuge tube and is fabricated of a material having a lower density than the liquid being carried therein to prevent bottoming of the adapter in the rotor cavity in the event of tube rupture.
  • an adapter formed of two piece construction is the device shown in United States Patent 3,674,197 (Mitchell et al.), assigned to the assignee hereof.
  • This adapter comprises two discrete segments, each of which has an indentation therein. When joined the indentations form a recess for receiving a collapsible bag during centrifugation.
  • the adapter disclosed in this patent includes aperture(s) through which tubes from the bag exit the adapter. Thus, the possibility exists that the bag may extrude through these apertures if the adapter were to undergo centrifugation in a vertical angle rotor.
  • An adapter arrangement formed of two discrete adapter segments and useful to support the capped end of a centrifuge tube is available as part of the Nalgene Ultra-Lok Tube System sold by Fisher Scientific Incorporated.
  • United States Patent 4,692,137 discloses a tube adapter having two segments which are hinged along the lateral edges of the segments.
  • the hinge axes align in parallel relationship to the axis of the cavity in which the adapter is received.
  • the disposition of hinges along the lateral edges of the segments is believed disadvantageous in that such a disposition may interfere with the insertion or removal of the adapter into or from the rotor cavity.
  • United States Patent 3,998,383 (Romanauskas et al.) and United States Patent 4,015,775 (Rohde), disclose centrifuge rotors of the vertical angle type. In such a rotor the axis of the rotor cavities is substantially parallel to the axis of rotation. When using a vertical angle rotor it is necessary that a cap be provided at the mouth of each cavity to impose a vertical restraining force on the tube disposed in the cavity. Even though the tube may be disposed in an adapter received within the cavity, without such a capping arrangement the possibility exists that the pressure of the liquid during centrifugation may rupture the tube.
  • United States Patent 3,998,383 (Romanauskas et al.) exemplifies a typical capping arrangement for a vertical angle rotor.
  • the present invention relates to an adapter for supporting a closed centrifuge tube in which at least one of the adapter segments has an effective weight sufficient to balance forces created by the pressure of a liquid carried in the tube under centrifugation that act transversely to the central axis.
  • the one segment is disposed closer to the axis of rotation so that the mating surfaces of the adapter segments lie in a plane that is perpendicular to a radius of the rotor extending through the cavity.
  • Suitable keying may be provided to identify the one segment having the predetermined effective weight.
  • An adapter may be fabricated from any suitable material so long as the resulting adapter has sufficient strength (as that term is defined herein).
  • the material of choice must exhibit other desirable properties, such as appropriate ultimate strength, appropriate modulus of elasticity, suitable chemical compatibility with any liquid sample being centrifuged and ability to withstand autoclaving.
  • Suitable plastic materials include polypropylene, polyamide, acetal, polyphenylene oxide, polyvinyl choloride, polycarbonate or polyethylene.
  • Other plastic or metallic materials either homogeneous (neat) or fiber reinforced with similar or better mechanical and chemical properties for the application under consideration may also be used.
  • the adapter may be formed in any convenient manner consistent with the material selected, such as molding, machining, casting or forging.
  • the adapter In order to support a tube T in a vertical angle rotor without the assistance of the restraining force provided by a capping mechanism, the adapter must exhibit sufficient strength to absorb the forces imposed on the tube T by the pressure of the liquid therein.
  • "sufficient strength" means that the adapter must be able to withstand the forces imposed on it during centrifugation without failing or deforming to the extent that the tube carried therein ruptures.
  • Whether a given adapter is of sufficient strength can be determined from various readily ascertainable operating parameters of the vertical angle rotor in which the adapter is to be used and the application to which the adapter is to be put. These parameters are the specific weight of the liquid sample within the tube received by adapter, the radius R i which represents the minimum distance to the sample from the axis A of rotation ( Figure 2A), the diameter D o ( Figure 2A) of the rotor cavity, the thickness of the adapter segment, the inside diameter of the tube, and the speed of rotation of the vertical angle rotor.
  • the total vertical force F V that the adapter must withstand is then found by integrating this pressure function over the circular cross sectional area of the inside of the tube.
  • the modulus of elasticity of that material may be readily obtained.
  • An estimation of the vertical deformation of the adapter may be found by multiplying the initial length of the adapter by the average stress divided by the modulus of elasticity of the adapter material. If the average stress calculated in Equation (2) is less than the ultimate strength of the adapter material, and the predicted deformation is less than the deformation that will cause first leakage in the tube carried within the adapter, then the given adapter is to be construed to have sufficient strength for at least one operating cycle. The determination of sufficient strength as set forth above under operating conditions will verify both the analysis and the conclusion of the sufficiency of strength of the adapter.
  • the adapter may be designed and fabricated such that, under centrifugation, the body force of one adapter segment is sufficient to balance the force created by the pressure of a liquid carried in the tube under centrifugation that acts transversely to the central axis.
  • the preferred form of such an adapter when in use the preferred form of such an adapter must be disposed within a cavity of a rotor in an orientation such that the mating surfaces of the adapter segments lie in a plane that is substantially perpendicular to a radius of the rotor extending through the cavity. In such an orientation the line of action of closure of the preferred form of such an adapter aligns with a radial line extending from the axis of rotation of the rotor to the center of the cavity in which the adapter is disposed.
  • Figure 1 illustrates a preferred arrangement of a split adapter 10 4 for use in a vertical angle rotor.
  • the adapter 10 4 includes a first adapter segment 12 4 and a second adapter segment 14 4 .
  • Each segment 12 4 , 14 4 has an exterior surface 16 4 and a mating surface 18 4 thereon.
  • the exterior surface 16 4 of each segment 12 4 , 14 4 is defined by a generally cylindrical lateral surface portion 20 4 and a planar upper surface portion 22 4 .
  • the exterior surface 16 4 of each segment 12 4 , 14 4 is sized and shaped for close fitting receipt within the cavity 40 V of a vertical angle rotor 42 V ( Figures 2A through 2C).
  • Each segment 12 4 , 14 4 is provided with an indentation 28 4 in the mating surface 18 4 thereof.
  • the indentation 28 4 in each segment 12 4 , 14 4 is shaped so that when the segments 12 4 and 14 4 are joined along their mating surfaces 18 4 the indentations 28 4 cooperate to define a recess 30 4 able to totally surround a centrifuge tube T disposed therein.
  • the adapter 10 4 has a central axis 10 4 A extending therethrough ( Figure 2A). As will be discussed later, the recess 30 4 may be inclined with respect to the central axis 10 4 A and remain within the contemplation of this invention.
  • the mating surfaces 18 4 of the segments 12 4 , 14 4 may be angled with respect to a reference plane.
  • the adapter 10 4 may be provided with a hinge on the upper surface 22 4 of the segments. If a hinge is provided, the pivotal axis of the hinge extends perpendicular to the axis of the adapter.
  • the hinge may take the form of a live hinge or a coined hinge.
  • At least one of the segments of the adapter 10 4 must have a predetermined effective weight under centrifugation that is sufficient to prevent separation of the adapter segments.
  • the effective weight of the adapter segment is defined as the weight of the segment at sea level multiplied by the g (gravity) force imposed on the segment when the same is rotated at a predetermined operating speed with the center of mass of the segment lying a predetermined radial distance from an axis of rotation.
  • At least one, but preferably, both of the segments 12 4 , 14 4 has at least one, but preferably, a pair of resilient extensions 58 4 , 60 4 , respectively, thereon.
  • the resilient extensions 58 4 , 60 4 are flexibly mounted, as by hinging, to the outside surface 16 4 of the segment along a line of bending 62 4 .
  • the resilient extensions 58 4 , 60 4 are biased to flare outwardly from the adapter segment, and are bendable along the line of bending 62 4 to close inwardly toward the lateral surface portion 20 4 of the outside surface 16 4 of the segment to which they are attached.
  • each extension 58 4 , 60 4 In the closed position the resilient extensions 58 4 , 60 4 are in contact with the lateral surface portion 20 4 of the outside surface 16 4 of the segment to which they are attached.
  • the lower end of each extension is tapered, as at 64 4 . It should be noted that when the adapter segments 12 4 , 14 4 are mated the edges of resilient extensions 58 4 , 60 4 are circumferentially spaced a slight distance apart, thereby to provide sufficient clearance to accommodate the flexing motion of the resilient extensions 58 4 , 60 4 during insertion into the rotor cavity.
  • each extension 58 4 , 60 4 serves to frictionally interact with the boundaries of a rotor cavity 40 V ( Figure 2A through 2C) to prevent rotation of the adapter 10 4 about its axis 10 4 A with respect to the body of the rotor 42 V thereby to maintain the adapter 10 4 in a predetermined angular orientation within the cavity during operation of the rotor.
  • FIGS 2A through 2C illustrate the adapter 10 4 of Figure 1 in use in a cavity 40 V of a vertical angle centrifuge rotor 42 V .
  • Sectioning of the adapter has been omitted from Figure 2C for clarity of illustration.
  • the segment for example, the segment 12 4
  • the other segment 14 4 which lies farther from the axis of rotation 10A is termed the "outboard” segment.
  • the inboard segment 12 4 must have an effective weight sufficient to balance the force F T created by the pressure of a liquid carried in the tube under centrifugation that acts transversely to the central axis 10 4 A of the adapter 10 4 .
  • the recess 30 4 may be arranged within the adapter 10 4 such that an axis extending centrally through the recess 30 4 of the adapter 10 4 is offset radially with respect to the axis 10 4 A of the adapter 10 4 .
  • the recess 30 4 is not concentric with the generally cylindrical lateral surface portion 20 4 of the adapter 10 4 . If so offset it is preferred that the recess 30 4 be displaced radially outwardly with respect to the axis 10 4 A of the adapter 10 4 .
  • Such an arrangement may be utilized to increase the effective weight of the inboard segment 12 4 and/or to dispose the sample to higher g-forces resulting from the increased radial distance to the sample.
  • Whether the inboard adapter segment 12 4 has an effective weight sufficient for the purpose of containing the transverse force F T can be determined from consideration of the identical operating parameters as previously developed and described in connection with the "sufficient strength" determination for accommodation of the vertical force F V .
  • the pressure P across the diameter of the tube is defined by Equation (1).
  • the value of the pressure P ranges from zero at the inboard edge of the tube to a maximum value at the farthest radial location of the liquid sample from the axis of rotation of the rotor.
  • the inboard segment 12 4 of the adapter 10 4 is subjected to a radially inwardly directed force F T that results from liquid pressure in the inboard half of the tube.
  • the magnitude of this radially inwardly directed force F T is determined by integrating the component of the pressure function defined by Equation (1) that is parallel to a radial line through the center of mass of the inboard segment 12 4 over the surface area of the indentation 28 4 of the adapter segment 12 4 .
  • the adapter 10 4 may be fabricated from any suitable material so long as the resulting adapter segment 12 4 has sufficient effective weight (as that term is defined herein) and exhibits suitable chemical compatibility with any liquid sample being centrifuged. It should preferably have the ability to withstand sterilization, as by autoclaving. Suitable plastic materials include polypropylene, polyamide, acetal, polyphenylene oxide, polyvinyl choloride, polycarbonate or polyethylene. Other plastic or metallic materials (either homogeneous (neat) or fiber reinforced) with similar or better mechanical and chemical properties for the application under consideration may also be used.
  • the adapter may be formed in any convenient manner consistent with the material selected, such as molding, machining, casting or forging.
  • both the segments 12 4 , 14 4 may be substantially identical in weight or they may be substantially different in weight, so long as the inboard adapter segment has the requisite effective weight to completely contain the tube T during operation of the rotor.
  • the adapter 10 4 also be fabricated of a material that has sufficient strength to withstand the vertical force F V due to liquid pressure under centrifugation.
  • a separate capping arrangement on the rotor is required.
  • the inboard segment 12 4 By providing the inboard segment 12 4 having a suitable effective weight the mating surfaces on the inboard and outboard segments remain in contact during operation of the rotor and no gap therebetween may form.
  • the tube T is thus completely contained within the conjoined adapter segments during operation of the rotor, and the possibility of tube failure due to extrusion into a gap is precluded.
  • the present invention carries the additional benefit of minimizing circumferential stress in the tube caused by the pressure of the liquid, therefore further reducing the possibility of tube failure. Since the effective weight of the inboard segment of the adapter is at least as great as the transverse force due to pressure F T , the inboard segment limits expansion of tube. Greater tube reliability over a greater range of tube, adapter and cavity tolerances is thus produced.
  • the adapter 10 4 must be disposed in the cavity 40 V of the rotor 42 V in an orientation which substantially aligns the line of action 48 of closure of the adapter segments 12 4 , 14 4 with a radial line extending from the axis of rotation of the rotor to the center of the cavity 40 V in which the adapter is disposed and which places the mating surfaces 18 4 of the adapter segments 12 4 , 14 4 in the plane 68 ( Figure 2A) that is perpendicular to the radius extending through the cavity 40 V .
  • the segments 12 4 , 14 4 may be keyed in a fashion to be described.
  • both segments 12 4 , 14 4 have the requisite effective weight sufficient to ensure complete containment of the tube in the adapter recess, then either segment may assume the position of the inboard segment.
  • the adapter may be inserted into the cavity in either of two different orientations and the desired performance will occur.
  • the keying can be implemented by providing any suitable distinctive physical feature on the adapter, such as a visually distinctive marking or a distinctive shape.
  • FIG. 3A and 3B An embodiment of the adapter 10 4 in which only one segment 12 4 exhibits the requisite effective weight is illustrated in Figures 3A and 3B.
  • the inboard adapter segment 12 4 has a distinctive configuration imparted thereto in the form of the flat surfaces 68 4 provided on the exterior surface 16 4 of the segment 12 4 .
  • the cavity 40 V into which the adapter 10 4 of Figure 3 is insertable is correspondingly shaped, thus to ensures that the adapter 10 4 is properly received into the rotor 42 V .
  • a portion of the upper surface 22 4 of the inboard segment 12 4 is removed to define a channel 70 4 therein.
  • the mating surfaces 18 4 on the inboard segment 12 4 are flush with the boundaries of the channel 70 4 .
  • the upper surface 22 4 of the outboard segment 14 4 is provided with a projecting flange 72 4 that is shaped in correspondence to the channel 70 4 .
  • the mating surfaces 18 4 on the outboard segment 14 4 are arranged to slidably engage the corresponding mating surfaces 18 4 on the inboard segment 12 4 .
  • the undersurface of the flange 72 4 has a pocket 76 4 therein that accepts the upper capped end of the tube T.
  • This structure defines a compact, cartridge-like adapter 10 4 for the tube T.
  • the tube T is retained in the outboard segment 14 4 with the capped end of the tube T received in the pocket 76 4 therein. This disposition is believed to facilitate handling of the tube T.
  • each adapter segment may be useful in converting a vertical angle rotor to a rotor having a "near vertical" cavity orientation.
  • the mating surfaces of the segments in such a case will lie in a plane that contains the axis of the recess, said plane also being inclined with respect to the axis of the adapter. These mating surfaces of such an adapter need not, therefore, align with the plane perpendicular to a radius extending from the axis of rotation of the rotor through the center of the cavity, as shown in Figures 1 through 3.
  • FIGs 4, 5, 6 and 7 illustrate another embodiment of a compact, cartridge-like adapter 10 4 similar to that shown in Figures 3A and 3B.
  • the adaptor 10 4 includes adapter segments 12 4 , 14 4 , each of which has an exterior surface 16 4 and a mating surface 18 4 thereon.
  • the exterior surface 16 4 has a cylindrical configuration similar to the adaptor of Figure 2C (without the resilient extensions 58 4 and 60 4 ) and is thus insertable in a correspondingly shaped cylindrical rotor cavity 40 V ( Figure 7).
  • the mating surfaces 18 4 include those planar surfaces of the adapter segments 12 4 , 14 4 that align in the plane 68 (perpendicular to the radius of the rotor 42 4 ) when the adapter is received within the rotor, as well as planar lateral surfaces that extend generally perpendicular thereto.
  • the former are indicated in the Figures by the character 18 4 and 18 4' respectively.
  • the mating surface 18 4 in each segment is provided with an indentation 28 4 that cooperate to define a recess 30 4 sized to accept a centrifuge tube T therein.
  • in the adapter 10 4 of Figures 4 through 7 only one segment, e. g., the inboard segment 12 4 , exhibits the requisite effective weight to insure that the mating surfaces 18 4 on the inboard segment 12 4 and the outboard segment 14 4 remain in contact during operation of the rotor so that no gap therebetween may form.
  • a portion of the upper surface 22 4 of the outboard segment 14 4 is removed to define the channel 70 4 therein.
  • the upper surface 22 4 of the inboard segment 12 4 is provided with the projecting flange 72 4 that is shaped in correspondence to the channel 70 4 .
  • the lateral portions 18 4' of the mating surfaces 18 4 on the inboard segment 12 4 are arranged to slidably engage the corresponding lateral portions 18 4' of the mating surfaces 18 4' on the outboard segment 14 4 .
  • the undersurface of the flange 72 4 may have a pocket 76 4 therein that accepts the upper capped end of the tube T.
  • the projecting flange 72 4 on the upper surface 22 4 of the inboard segment 12 4 has gripping serrations 80 4 provided thereon. These gripping serrations 80 4 facilitate manipulations of the segments.
  • the presence of the serrations 80 4 serves as a visual indicator key which both identifies the inboard segment and aligns the mating surfaces 18 4 of the adapter segments 12 4 , 14 4 in the plane 68.
  • a portion 82 4 of the flange 72 4 also projects forwardly thereby to overhang exterior surface 16 4 on the inboard segment 12 4 .
  • the segments 12 4 , 14 4 may be hinged. Since the serrated flange 72 4 is located at the upper end of the cartridge adaptor 10 4 a more convenient form of hinge arrangement 26 4 is shown in Figures 4 and 5.
  • the hinge arrangement 26 4 includes a pair of stub-like axles 86 4 disposed opposed lateral surface portions 18 4 of the mating surface 18 4 of the inboard segment 12 4 .
  • the hinge arrangement 26 4 further includes a pair of trunnion recesses 88 4 provided on the opposed confronting lateral surface portions 18 4' of the mating surface 18 4 of the outboard segment 14 4 .
  • the stub-like axles 86 4 on the inboard segment 12 4 snappingly engage into the trunnion recesses 88 4 on the outboard segment 14 4 .
  • the axis 26A 4 of the hinge 26 4 is perpendicular to the axis of the adapter 10 4 and supports the relative pivotal movement, about the hinge axis 26A 4 of the segment 12 4 , 14 4 with respect to the other from an open to a mated position.
  • the adapter 10 4 of Figures 4-7 may be fabricated of the same material as used for the adapter 10 4 of Figures 3A, 3B.

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Claims (21)

  1. Utilisation d'un raccord (104) pour supporter un tube centrifuge clos (T) à l'intérieur d'une cavité (40V) d'un rotor (42V), ledit raccord (104) comprenant :
    un premier segment intérieur de raccord (124) et un deuxième segment extérieur de raccord (144), chaque segment (124, 144) ayant une surface extérieure (164) et une surface d'accouplement (184), chaque segment (124, 144) ayant une entaille (284) dans la surface d'accouplement (184), ladite entaille (284) de chaque segment (124, 144) ayant une forme telle que lorsque les segments (124, 144) sont unis le long de leurs surfaces d'accouplement (184, 184), les entailles (284, 284) coopèrent pour définir un évidement (304) capable d'entourer totalement un tube centrifuge (T) disposé à cet endroit, le raccord (104) ayant un axe central (104 A) qui s'étend à travers,
    caractérisé par:
    l'utilisation d'un premier segment intérieur du raccord (124) ayant un poids utile suffisant pour équilibrer les forces créées par la pression d'un liquide se trouvant à l'intérieur du tube (T) faisant l'objet de la centrifugation qui agit de façon transversale par rapport à l'axe central (104 A),
    de façon à ce que, lors de l'utilisation avec le raccord (104) inséré dans une cavité (40V) d'un rotor (42V), les surfaces d'accouplement (184, 184) des segments du raccord (124, 144) étant dans une relation de contact l'une avec l'autre, le poids utile du segment intérieur (124) pendant la centrifugation est suffisant pour maintenir les surfaces d'accouplement (184, 184) des segments du raccord (124, 144) dans une telle relation de contact.
  2. L'utilisation de la revendication 1, selon laquelle le segment extérieur (144) a un poids utile prédéterminé et
    selon laquelle le poids utile du segment intérieur (124) n'est pas le même que le poids utile du segment extérieur (144).
  3. L'utilisation de la revendication 2, selon laquelle le poids utile du segment intérieur (124) est supérieur à celui du segment extérieur (144).
  4. L'utilisation de la revendication 1, selon laquelle le segment extérieur (144) a un poids utile prédéterminé et selon laquelle le poids utile du segment intérieur (124) est le même que le poids utile du segment extérieur (144).
  5. L'utilisation de la revendication 1 ou 2, selon laquelle l'un des segments (124, 144) a une caractéristique qui le distingue.
  6. L'utilisation de la revendication 5, selon laquelle la caractéristique qui distingue le segment est une caractéristique visuelle.
  7. L'utilisation de la revendication 5, selon laquelle la caractéristique qui distingue le segment est la forme dudit segment.
  8. L'utilisation de la revendication 5, selon laquelle la caractéristique qui distingue le segment est située sur le segment intérieur (124).
  9. L'utilisation de la revendication 8, selon laquelle la caractéristique qui distingue le segment est une marque visuelle.
  10. L'utilisation de la revendication 8, selon laquelle le segment intérieur (124) a une forme prédéterminée, et selon laquelle la caractéristique qui le distingue est la forme du segment intérieur.
  11. L'utilisation de l'une des revendications 1-10, selon laquelle la cavité (40V) du rotor (42V) est circulaire et selon laquelle chacun des segments (124, 144) a une surface extérieure (164), et au moins un des segments (124) a au moins une première extension élastique (584), ladite extension élastique (584) étant inclinée de façon à s'évaser vers l'extérieur à partir du segment du raccord (124), l'extension élastique (584) pouvant être recourbée par rapport au segment du raccord (124) depuis la position évasée jusqu'à une deuxième position dans laquelle l'extension (584) est en contact avec la surface extérieure (164) du segment (124) sur lequel elle est disposée, et selon laquelle
    lorsque le raccord (104) est disposé dans un rotor (42V), l'extension (584) est en interaction par friction avec les frontières d'une cavité du rotor (40V) afin d'empêcher la rotation du raccord (104) autour de son axe (104 A) par rapport au corps du rotor (42V), ce qui permet de maintenir le raccord (104) dans une orientation angulaire prédéterminée à l'intérieur de la cavité (40V) pendant que le rotor (42V) tourne.
  12. L'utilisation de la revendication 11, selon laquelle l'extension (584, 604) est attachée au segment (124, 144) par une charnière le long d'un axe de pliage (624) disposé sur la surface extérieure (164) du segment (124, 144).
  13. L'utilisation de la revendication 12, selon laquelle l'extension (584, 604) a une de ses parties qui est conique (644).
  14. Le raccord de l'une des revendications 1-13, selon laquelle le segment intérieur (124) a une surface extérieure (164), ladite surface extérieure (164) ayant au moins une clavette planaire (684) qui y est définie.
  15. L'utilisation de la revendication 14, selon laquelle le segment intérieur (124) et le segment extérieur (144) ont tous les deux une surface supérieure (224), le segment intérieur (124) ayant un canal (704) formé à travers la surface supérieure (224), le segment extérieur (144) ayant une bride (724) formée de façon à correspondre au canal (704) disposé sur sa surface supérieure (224), la bride (724) ayant une poche (764) formée à l'intérieur de celle-ci, ladite poche (764) ayant une taille appropriée pour recevoir le bout d'un tube (T) à l'intérieur du raccord (104).
  16. L'utilisation de l'une des revendications 1-15, selon laquelle le rotor centrifuge (42V) est un rotor à angle vertical, et selon laquelle les segments du raccord (124, 144) sont fabriqués en un matériau qui a une force suffisante pour résister aux forces verticales créées par la pression d'un liquide faisant l'objet de la centrifugation.
  17. L'utilisation de la revendication 1 ou 16 comprenant en plus au moins une charnière (264) connectant les segments (124, 144) et supportant le mouvement de pivotement relatif autour d'un axe de charnière (264 A) d'au moins un segment (124) par rapport à l'autre (144) d'une position ouverte à une position accouplée, l'axe de la charnière (264 A) s'étendant perpendiculairement à l'axe du raccord (104 A).
  18. L'utilisation de la revendication 17, selon laquelle la charnière (264) comprend deux essieux (864) formés à l'extrémité inférieure d'un segment (124) et deux évidements à pivots (884) formés à un endroit correspondant sur l'autre segment (144).
  19. L'utilisation de la revendication 2, selon laquelle le poids utile du segment extérieur (144) est supérieur à celui du segment intérieur (124).
  20. L'utilisation de l'une des revendications 1-19, selon laquelle le segment intérieur (124) a une bride (724) et le segment extérieur (144) a un canal (704) de taille appropriée pour recevoir la bride (724), ladite bride (724) étant caractérisée par la présence de dentelures (804).
  21. L'utilisation de la revendication 20, selon laquelle la bride (724) sur le segment intérieur (124) a une saillie suplombante (824) adaptée de manière à reposer contre une étagère pratiquée dans un rotor centrifuge (424).
EP92913835A 1991-05-06 1992-05-06 Adaptateur pour tube de centrifugation Expired - Lifetime EP0584277B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US69587191A 1991-05-06 1991-05-06
US695871 1991-05-06
PCT/US1992/003813 WO1992019382A1 (fr) 1991-05-06 1992-05-06 Adaptateur pour tube de centrifugation

Publications (3)

Publication Number Publication Date
EP0584277A1 EP0584277A1 (fr) 1994-03-02
EP0584277A4 EP0584277A4 (fr) 1994-11-30
EP0584277B1 true EP0584277B1 (fr) 1998-01-28

Family

ID=24794774

Family Applications (1)

Application Number Title Priority Date Filing Date
EP92913835A Expired - Lifetime EP0584277B1 (fr) 1991-05-06 1992-05-06 Adaptateur pour tube de centrifugation

Country Status (5)

Country Link
EP (1) EP0584277B1 (fr)
JP (1) JP2777283B2 (fr)
CA (1) CA2102320A1 (fr)
DE (1) DE69224289T2 (fr)
WO (1) WO1992019382A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5295943A (en) * 1989-11-07 1994-03-22 E. I. Du Pont De Nemours And Company Adapter for holding a pair of centrifuge tubes
US5935052A (en) * 1993-05-27 1999-08-10 Sorvall Products, L.P. Adapter for centrifuge tube
JP2011125813A (ja) * 2009-12-18 2011-06-30 National Cancer Center 遠心分離用容器、遠心分離用容器の姿勢保持用アダプター及び遠心分離用用具
DE102014008445A1 (de) * 2014-06-06 2015-12-17 Thermo Electron Led Gmbh Adapter für einen Probenbehälter einer Zentrifuge sowie Adaptergruppe und Zentrifugiersystem
JP6431438B2 (ja) * 2015-04-28 2018-11-28 株式会社フコク 遠心分離用容器の姿勢保持用アダプタ

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3674197A (en) * 1970-09-08 1972-07-04 Sorvall Inc Ivan Washing means for flexible bags in split enclosures
US4306676A (en) * 1980-04-17 1981-12-22 Beckman Instruments, Inc. Tube holder for centrifuge rotor
US4692137A (en) * 1985-04-03 1987-09-08 Beckman Instruments, Inc. Split tube centrifuge rotor adapter

Also Published As

Publication number Publication date
EP0584277A1 (fr) 1994-03-02
DE69224289T2 (de) 1998-09-10
DE69224289D1 (de) 1998-03-05
EP0584277A4 (fr) 1994-11-30
WO1992019382A1 (fr) 1992-11-12
JP2777283B2 (ja) 1998-07-16
CA2102320A1 (fr) 1992-11-07
JPH06511187A (ja) 1994-12-15

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