EP0542753A1 - Epitope der prä-s-region des hepatitis-b-virus oberflächenantigens - Google Patents
Epitope der prä-s-region des hepatitis-b-virus oberflächenantigensInfo
- Publication number
- EP0542753A1 EP0542753A1 EP19910910584 EP91910584A EP0542753A1 EP 0542753 A1 EP0542753 A1 EP 0542753A1 EP 19910910584 EP19910910584 EP 19910910584 EP 91910584 A EP91910584 A EP 91910584A EP 0542753 A1 EP0542753 A1 EP 0542753A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- hbsag
- polypeptide
- amino acid
- acid residue
- cell epitope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Definitions
- polypeptides referred to as p39, p33 and p25 are polypeptides referred to as p39, p33 and p25,
- the antigens tested for induction of T cell proliferation included pre-S(2)-containing
- substitutions have been made, usually not more than about 20% and more frequently not more than 10% of the amino acid residues differ.
- sequences which "substantially correspond" to a specified sequence are those having not more than 10% deletions, insertions or substitutions.
- additional residues may be added at either terminus.
- the additional residues may correspond to contiguous residues in the linear sequence of HBsAg as described previously, or may be repeats of the sequence or amino acid residues that are unrelated to HBsAg.
- vaccine in its various grammatical forms is used herein to describe a type of inoculum containing one or more polypeptide of this invention as an active ingredient that is used to induce active immunity in a host mammal against HBV. Since active immunity involves the production of antibodies, a vaccine or inoculum may thus contain identical
- a plurality of T cell epitopes of each subtype are administered.
- HBsAg sandwich enzyme-linked immunosorbent assay
- Synthetic polypeptides having a sequence corresponding to p133-174/ad or p131-174/ad were prepared and injected into mice as described above.
- the induced antibody was analyzed by ELISA as
- H-2 haplotypes H-2 f,s
- H-2 t4 H-2 haplotypes
- a nonresponding haplotype following HBsAg/p39 immunization has not yet been identified amongst the following H-2 haplotypes studied to date: q, d, s, k, b, p, f, t4.
- FIG. 5 demonstrates that T cell recognition in a variety of strains is focused on the C-terminal half of the pre-S (2) region.
- HBsAg/P33d-primed T cells from a number of strains were cultured with full length (P33) or N-terminally truncated (P28) HBsAg particles. Because the truncated version elicited significant T cell proliferation in all strains immunized with HBsAg/P33, it appears that the N- terminal residues (120-139) do not play a significant role in T cell recognition.
- B10 HBsAg/P33-primed T cells react efficiently with HBsAg/P28 (the truncated sequence), p141-160 and p146-165, consistent with the dominant T cell site, p149-157, which was also defined with synthetic peptides p133-174 and p148-174 as immunogens.
- B10.M HBsAg/P33-primed T cells react efficiently with HBsAg/P28, p151-170, and p156-174, consistent with the dominant site p156-167, which was also defined with synthetic peptides p133- 174 and p148-174 as the immunogens (Figure 11B).
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52266390A | 1990-05-11 | 1990-05-11 | |
| US522663 | 1990-05-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP0542753A1 true EP0542753A1 (de) | 1993-05-26 |
| EP0542753A4 EP0542753A4 (en) | 1993-09-08 |
Family
ID=24081802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP19910910584 Withdrawn EP0542753A4 (en) | 1990-05-11 | 1991-05-10 | Epitopes of the pre-s region of hepatitis b virus surface antigen |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0542753A4 (de) |
| AU (1) | AU7909391A (de) |
| IE (1) | IE911614A1 (de) |
| PT (1) | PT97632A (de) |
| WO (1) | WO1991017768A1 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5759551A (en) * | 1993-04-27 | 1998-06-02 | United Biomedical, Inc. | Immunogenic LHRH peptide constructs and synthetic universal immune stimulators for vaccines |
| WO1998012332A1 (en) | 1996-09-17 | 1998-03-26 | Chiron Corporation | Compositions and methods for treating intracellular diseases |
| DE10339927A1 (de) * | 2003-08-29 | 2005-03-24 | Rhein Biotech Gesellschaft für neue Biotechnologische Prozesse und Produkte mbH | Zusammensetzung zur Prophylaxe/Therapie von HBV-Infektionen und HBV-vermittelten Erkrankungen |
| US20220411475A1 (en) * | 2019-11-18 | 2022-12-29 | Vlp Biotech, Inc. | Hybrid virus-like particles and use thereof as a therapeutic hepatitis b vaccine |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987007896A1 (en) * | 1986-06-20 | 1987-12-30 | Scripps Clinic And Research Foundation | T and b cell epitopes of the pre-s region of hepatitis b virus surface antigen |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935235A (en) * | 1979-05-24 | 1990-06-19 | The Regents Of The University Of California | Non-passageable viruses |
| US4847080A (en) * | 1984-03-07 | 1989-07-11 | New York Blood Center, Inc. | Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipide vesicle carriers |
| US4861588A (en) * | 1985-02-05 | 1989-08-29 | New York Blood Center, Inc. | Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers |
| US4683136A (en) * | 1985-03-06 | 1987-07-28 | Scripps Clinic And Research Foundation | Proteinaceous antigens with conformation-independent and conformation-dependent determinants |
| US4882145A (en) * | 1986-12-09 | 1989-11-21 | Scripps Clinic And Research Foundation | T cell epitopes of the hepatitis B virus nucleocapsid protein |
-
1991
- 1991-05-09 PT PT97632A patent/PT97632A/pt not_active Application Discontinuation
- 1991-05-10 IE IE161491A patent/IE911614A1/en unknown
- 1991-05-10 AU AU79093/91A patent/AU7909391A/en not_active Abandoned
- 1991-05-10 EP EP19910910584 patent/EP0542753A4/en not_active Withdrawn
- 1991-05-10 WO PCT/US1991/003268 patent/WO1991017768A1/en not_active Application Discontinuation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987007896A1 (en) * | 1986-06-20 | 1987-12-30 | Scripps Clinic And Research Foundation | T and b cell epitopes of the pre-s region of hepatitis b virus surface antigen |
Non-Patent Citations (4)
| Title |
|---|
| ANN. INST. PASTEUR VIROL. vol. 139, no. 1, 1988, pages 13 - 38 A. NEURATH ET AL. 'Delineation of contiguous determinants essential for biological functions of the pre-s sequence of hepatitis B virus envelope protein' * |
| PROC. NATL ACAD. SCI. USA vol. 85, 1988, pages 1610 - 1614 D. MILICH ET AL. 'Hepatitis B synthetic immunogen composed of nucleocapsid T-cell sites and an envelope B cell epitope' * |
| PROC. NATL. ACAD. SCI. USA vol. 86, 1989, pages 9084 - 9088 J. TAM 'Vaccine engineering: enhancement of immunogenicity of synthetic peptide vaccines' * |
| See also references of WO9117768A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| IE911614A1 (en) | 1992-09-23 |
| AU7909391A (en) | 1991-12-10 |
| PT97632A (pt) | 1992-03-31 |
| EP0542753A4 (en) | 1993-09-08 |
| WO1991017768A1 (en) | 1991-11-28 |
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