EP0538450A1 - Procede et appareil permettant de detecter la contamination bacterienne du sang destine aux transfusions - Google Patents

Procede et appareil permettant de detecter la contamination bacterienne du sang destine aux transfusions

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Publication number
EP0538450A1
EP0538450A1 EP92911615A EP92911615A EP0538450A1 EP 0538450 A1 EP0538450 A1 EP 0538450A1 EP 92911615 A EP92911615 A EP 92911615A EP 92911615 A EP92911615 A EP 92911615A EP 0538450 A1 EP0538450 A1 EP 0538450A1
Authority
EP
European Patent Office
Prior art keywords
bag
sensor
microbial growth
blood
growth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP92911615A
Other languages
German (de)
English (en)
Other versions
EP0538450A4 (fr
Inventor
Roger J. Morris
Calvert Lawrence Green
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter Healthcare Corp
Original Assignee
Baxter Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Diagnostics Inc filed Critical Baxter Diagnostics Inc
Publication of EP0538450A1 publication Critical patent/EP0538450A1/fr
Publication of EP0538450A4 publication Critical patent/EP0538450A4/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Definitions

  • This invention relates to a noninvasive method and apparatus to detect the presence or determine the concentration of microorganisms in a container of transfusable blood prior to transfusion.
  • Microorganisms present in bodily fluid can be detected using a culture bottle.
  • a culture bottle is a flask allowing positive cultures to be detected rapidly.
  • the flask is generally a transparent closed container filled with nutrient that promotes the growth of the organism.
  • bacteria in blood can be detected in culture.
  • U.S. Patent No. 4,772,55_A-(Hammann) . Many different qualitative and quantitative detection means are used to monitor the growth of microorganisms in a culture bottle.
  • the microorganisms in a culture bottle have been detected by use of external detectors such as a magnifying lens, U.S. Patent No. 4,543,907 (Freudlich) .
  • liquid level indicators can show bacterial growth as a function of increased pressure in the vessel.
  • microorganisms can be detected by measuring changes in pH caused by bacterial growth, Mariel, G.B. Patent No. 1,601,689.
  • Still another method to detect microorganisms involves the use of a culture media that contains a compound which changes color or appearance according to the growth of microorganisms.
  • the change in the media can be detected with a spectrophotometer.
  • Bascomb Enzyme Tests in Bacterial Identification, Meth. Microbiol. 19 . , 105 (1987) .
  • a variety of organisms can be classified in large part by their pattern of fermentation, oxidation or assimilation of carbon sources. Fermentation of carbohydrates results in the production of acid which causes a decrease in pH. This drop in pH can be easily detected by including a pH indicator like bromthymol blue or phenol red.
  • Chemical and enzymatic reactions are used to detect or quantitate the presence of certain substances in microbiological or other assays. Many of these tests rely on the development or change of color or fluorescence to indicate the presence or quantity of the substance of interest.
  • Another approach to determine if an organism can degrade a particular substrate is to use a reagent which is capable of reacting with one or more of the intermediates or final products. For example, the detection of the reduction of nitrate to nitrite. If nitrite is formed, then a pink to deep red color will result when sulfanilic acid and alpha-naphthylamine are added to the reaction mixture.
  • a synthetic analog of a natural substrate can be used directly indicate the presence of an enzyme.
  • methylene blue can be reduced under certain conditions by the action of reductase, resulting in a shift from blue to colorless.
  • the oxidase assay relies on the interaction of cytochrome oxidase with N, N, N' , N l -tetramethyl- / /-phenylene-diamine producing a blue color.
  • Another example is the ability of microorganisms to degrade sulfur-containing a ino acids as indicated by the production of H 2 S.
  • the organism is incubated with a high concentration of a sulfur-containing substrate (e.g. cysteine, cystine) in an acid environment.
  • a sulfur-containing substrate e.g. cysteine, cystine
  • the production of H 2 S is indicated by the formation of a black precipitate in the presence of ferric ammonium citrate.
  • Enzymes can usually act on more than one substrate. This allows for the use of synthetic enzyme substrates for the detection of enzyme activities.
  • Synthetic substrates contain a metabolic moiety conjugated with a chromatic or fluorescent moiety.
  • the conjugated molecule usually has a different absorption and/or emission spectrum from the unconjugated form.
  • the unconjugated chromatic or fluorescent moiety shows a considerably higher absorption or fluorescence coefficients than those of the conjugated molecule.
  • An example of a synthetic enzyme substrate ⁇ is _-nitro-phenol-jS-galactopyranoside used for the detection of activity of the enzyme .-galactosidase.
  • the conjugated substrate is colorless.
  • the 3-galactosidase enzyme hydrolyzes the substrate to yield /_-galactosidase and t-nitro-phenol.
  • Bascomb, Enzyme Tests in Bacterial Identification, Meth. Microbiol. JL9 . , 105 (1987) reviewed the synthetic moieties used for enzyme substrates and the enzymatic activities measurable using this principle.
  • the monitoring of color or color end-product in chemical and microbial reactions is usually achieved in either of two ways; 1) the detection of color or color end-product can be achieved by visual observation and estimated qualitatively, or 2) the detection of color end-products or loss of color can be achieved by measuring the intensity of color instru entally.
  • Spectrophotometers that measure light absorbance are commonly used for this purpose. When measuring the concentration of a number of substances it is advantageous to use one instrument based on one principle of measurement, otherwise cost is increased. Although the use of colorimetric reactions is widespread there are limitations, especially in the sensitivity of detection. In order to improve sensitivity and, in the case of identification of microorganisms, thereby to decrease the time required to obtain a result, fluorescence-based methods frequently are used. Unfortunately, it may not be possible to develop a fluorescent equivalent to every assay. Additionally, the fluorescent reagents , themselves may be highly toxic and therefore difficult to commerciali,ze.
  • the general principle of fluorescence quenching has been accepted as a way to detect or determine enzymatic or chemical reactions.
  • Fleminger et al. synthesized intramolecularly quenched fluorogenic substrates for the assay of bacterial aminopeptidase
  • P. Fleminger et al. Fluoroqenic Substrates for Bacterial Aminopeptidase P and its Analog's Detected in Human Serum and Calf Lung. Eur. J. Biochem. 125, 609 (1982) .
  • the fluorescence of the aminobenzoyl group is quenched by the presence of a nitrophenylalanyl group.
  • Enkephalinase a Neutral Metalloendopeptidase that Releases Tyrosine-Glvcine-Glvcine from Enkephalins, Anal. Biochem. 141, 62 (1984) .
  • a synthetic substrate containing a quenching group and a fluorescing group was generated in order to detect the activity of the enzyme.
  • An alternative to this approach would involve the synthesis of a resonance energy transfer pair of fluorescing groups on a substrate molecule. In this method, cleavage by the enzyme of one of the groups would result in a decrease in fluorescence, since the critical distance would be exceeded, eliminating the transfer of energy.
  • the previously discussed approaches are limited to specifically designed substrates.
  • Still another approach involves the estimation of a chromophore by fluorescence measurement. See . Blu berg et al. , Hemoglobin Determined in Whole Blood "Front Face” Fluorometry, Clin. Hemo. 2 _, 409 (1980) . Blumberg disclosed an assay based on attenuation of fluorescence of a dye, whose excitation wavelengths overlap with the absorption wavelengths of the chromophore.
  • Shaffer U.S. Patent No. 4,495,293
  • Shaffer filed a patent application disclosing a method to fluorometrically determine a ligand in an assay solution using conventional fluoro etric techniques.
  • the intensity of the fluorescence emitted by the assay > solution is related to the change in trans issive properties of the assay solution produced by the interaction of ; the ligand to be determined and a reagent system capable of producing change in the transmissive properties of the assay solution in the presence of the ligand.
  • Shaffer discloses a method to monitor absorbance using a fluorophore in solution with the chromophore.
  • the fluorophore may interact with the assay cocktail and produce changes in fluorescence intensity which are unrelated to the change being measured.
  • the selection of the fluorophores is also restricted, in that pH dependent or environment sensitive fluorophores cannot be utilized. Additionally, when the fluorophore is in solution, less than accurate measure of absorbance may be obtained because light is absorbed exponentially through the chromophore sample.
  • Beggs & Sand, EPA 91,837 disclosed a solution based method for determination of tryptophan-deaminase activity by measuring the reduction in fluorescence in the presence of a chromophore produced by the interaction between indole pyruvic acid and metal ions using a fluorophore "whose fluorescence is capable of being quenched by the indole pyruvate-metal ion complex, the ions of the fluorophore being present throughout the incubation period".
  • Sands, U.S. Patent No. 4,798,788 discloses a process to detect a nitrate reducing microorganism by measuring reduction of fluorescence in solution by causing the diazotization of the fluorophore.
  • a specific fluorophore needs to be chosen for each test to ensure that it will fluoresce under the conditions of the test, e.g. only few fluorophores fluoresce at pH of ,less than 2.0.
  • the previously discussed blood culture test can be used to determine bacterial contamination of transfusable blood, these test may result in errors.
  • the transfusion bag must be later matched with a separate blood culture bottle sent to a test center to make a determination of potential microbial contamination prior to transfusion of the blood. This requirement for subsequent matching could result in errors.
  • blood culture bottles are cultured at higher temperature than the temperature that blood is normally stored; as such blood culture bottle tests yield an accelerated picture of bacterial contamination, while a test that simulates actual blood storage conditions may yield more accurate results.
  • This invention relates to a multi-layer body fluid culture sensor comprised of a pH sensitive absorbance based dye spectrally coupled to a pH insensitive, or pH sensitive dye that is highly buffered, fluorescence based dye.
  • the pH sensitive absorbance based dye is encapsulated or isolated in a polymeric layer that is permeable to C0 2 and water, but impermeable to protons.
  • the pH insensitive fluorophore is encapsulated or isolated in the second polymeric layer that may or may not be permeable to C0 2 and water.
  • This type of sensor may be used to detect or determine the concentration of , microorganisms in bodily fluid.
  • the spectral criterion required to make this determination are such that the absorption spectrum of the chromophore must overlap the excitation and/or emission spectrum of the fluorophore, thereby allowing the change in fluorescence to be related to the change in the reaction and consequently related to the presence or quantity of the substance of interest.
  • this sensor is used to monitor microbial growth in collected transf sable blood.
  • this sensor can be used to monitor bacterial growth in a collection bag of bodily fluid that is to be transfused into a patient.
  • bacteria grow they generate C0 2 .
  • the C0 2 generated by the bacteria diffuses into the polymeric layer that is in direct contact with a hydrated pH sensitive absorbance based dye.
  • the C0 2 reacts with the aqueous environment to form carbonic acid (H 2 C0 3 ) , which lowers the pH of the absorbance dye environment. This results in a concomitant change in the pH sensitive spectrum of the dye.
  • the senor is attached to a blood collection bag or separate sampler test bag. If a separate sampler bag is used this bag may contain microbial growth media or an inert substance such as a saline. With this system microbial contamination of transfusable blood in a collection bag can be determined immediately prior to transfusion.
  • a detector such as a handheld fluorescence detector, is used to monitor the - emitted fluorescence.
  • Fig. 1 shows a schematic diagram of a multi-layer blood culture sensor.
  • Fig.2 shows a blood culture growth curve detected by a xylenol blue-rhodamine 101 sensor.
  • Fig. 3 shows a blood culture growth curve detected by xylenol blue in silicone-rhodamine B in acrylic sensor.
  • Fig. 4 shows a blood culture growth curve for a xylenol blue in silicone-6213 acrylic sensor.
  • Fig. 5 shows a blood culture growth curve for a bromthymol blue in silicone-rhodamine 101 in silicone sensor.
  • Fig. 6 shows a sample test blood collection bag and fluorescent detector for monitoring growth of microorganisms in blood.
  • Fig. 7 shows the percent change of fluorescent intensity versus time for two blood samples.
  • fluorescence from a fluorophore embedded in an inert light-transparent matrix is modulated by a pH sensitive absorbance dye embedded in a polymeric gas permeable, but proton impermeable matrix.
  • the assay is carried out in a blood collection bag or sampler test blood collection bag.
  • fluorometric based colorimetric assay the fluorescence intensity is regulated by changes in absorbance of an interfering chromophore. As a pH change occurs the chromophoric material alters the ,amount of emitted light reaching the fluorophore and/or the amount of emitted light reaching the detector.
  • Spectrally compatible fluorescent and colorimetric indicators are selected so that as the pH changes due to the production of CO- by microorganisms present in the blood, the colorimetric indicators regulate the amount of light reaching the fluorophore and/or photodetector and, thus cause a change in the excitation and/or emission of the fluorescent dye. This change is detected with a fluorescent reader and can be correlated with the presence or concentration of microorganisms in the blood.
  • a bodily fluid culture sensor is comprised of a pH sensitive absorbance based dye in or isolated by a polymeric gas permeable, but proton impermeable matrix, and a fluorescent dye in a second polymeric matrix.
  • Spectrally compatible fluorescent and colorimetric indicators are selected so that when an organism is present in blood, the colorimetric indicator will regulate the amount of light reaching the fluorophore thereby causing a change in the emission intensity from the fluorescence dye reaching the photodetector.
  • the change indicating the presence of bacteria, is detected with a fluorometric reader.
  • spectrally compatible fluorescence and absorbance dyes are selected dyes are selected so that as carbonic acid is produced (C0 2 and H 2 0) , the absorbance of the dye will change thereby regulating the amount of light reaching the fluorophore and/or photodetector, thus producing a change in the measured fluorescence. This change is detected with a fluorescence reader.
  • Spectrally compatible dyes are rhodamine B and xylenol blue.
  • bromthymol blue and rhodamine 101 are also spectrally compatible. For example this can be illustrated by inoculating a bag containing the appropriate growth media with Yersinia enterocolitica. As the organism grows, it produces C0 2 gas.
  • the silicone is permeable to the C0 2 .
  • the C0 2 diffuses to the absorbance layer and reacts with water to produce carbonic acid (H 2 C0 3 ) .
  • the carbonic acid causes a drop in the pH in the absorbance dye environment resulting in a change in measured absorbance. For example, as the pH drops in an absorbance layer containing the dye xylenol blue, the absorbance of xylenol blue decreases, thereby allowing more light to reach the fluorophore to excite it and thus increase the amount of fluorescence emitted at 590nm.
  • a positive culture using xylenol blue as the absorbance dye is detected by increase the amount of fluorescence emitted at 590nm.
  • a positive culture using xylenol blue as the absorbance dye is detected by a measured increase in fluorescence as the xylenol blue decreases in absorbance See Fig. 7.
  • the pH sensitive absorbance based dye is encapsulated in or isolated by a polymeric matrix that is gas permeable, but proton impermeable.
  • the polymeric matrix must be optically transparent in the visible region, permeable to gas, autoclavable, stable for at least six months, and proton impermeable.
  • silicone may function as the polymeric matrix used to encapsulate or isolate the absorbance - based dye. Silicones found to meet these criteria , were Dow, Rhone Poulenc, G.E. and Wacker.
  • the fluorescence based dyes can also be encapsulated in a polymeric matrix.
  • the polymeric matrix used for the fluorophore does not have to meet all of the above requirements listed for the matrix used to encapsulate or isolate the absorbance dye.
  • the similar features that it must possess are that it must be optically transparent in the visible region, autoclavable and stable for at least six months.
  • the polymeric matrix containing or isolating the absorbance based dye must be coupled to the polymeric matrix containing the fluorescent dye. It should be noted that the polymeric matrices must be in close proximity so that light that has been regulated by the absorbance layer will have an effect on the emission intensity of the fluorophore as received by the photodetector.
  • a microorganism growth monitoring system for collected transfusable blood is shown in Fig. 6.
  • the monitoring system shown in Fig. 6 is comprised of a sampler test blood collection bag 20.
  • a bar code 28 can be attached to the bag to record data for later inspection. Blood from the blood collection bag to be transfused is expressed through tube 24 to sampler test blood collection bag 20.
  • the wall of the blood collection bag contains a multi-layer sensor 22 .comprising a pH sensitive dye in a light transmissive, gas permeable, proton impermeable matrix and a pH insensitive fluorescence dye in inert light transparent matrix, said first and second matrices being spectrally coupled.
  • the blood collection or blood storage bag can contain whole blood, plasma, serum, erythrocytes, red blood corpuscles, leukocytes, white blood corpuscles, thrombocytes and blood platelets, collectively referred to as blood storage.
  • the sensor can be located on the interior wall of the blood collection bag or a separate sampler test blood collection bag to which blood can be shunted for assessment. These various types of bags are heretofore collectively referred to as blood collection bag.
  • the bag may contain a growth media or an inert substance such as saline.
  • the two-layer sensor is mounted inside a blood collection bag such that one layer, of the sensor is positioned facing outside the bag.
  • the second layer which is fluorescent is positioned facing the interior of the bag.
  • the sensor may be formed integrally with the wall of the bag.
  • the invention is comprised of the two-layer sensor outlined above, mounted inside a blood collection container in such a way that by utilizing a fluorometer to excite the fluorescent sensor and detect the emitted fluorescent light, a determination can be made as to the presence of a threshold level of microorganisms contained within the blood collection container.
  • An additional feature of this invention is ; that the bag is stored at normal blood storage temperatures, i.e. 4°C.
  • certain microorganisms are not affected by the cold: Yersinia enterocolitica and Enterobacter agglomerans.
  • the system allows for the determination of other bacteria that would not normally grow rapidly in the cold but might be present in such high concentrations over time that it would be unsafe to transfuse into a patient: Citrobacter freundii; Pseudomonas aeruginosa; Staphylococcus aureus; and staphylococcus epidermidis.
  • An alternative approach would follow the same design but would consist solely of an absorbance sensor.
  • Another feature of this invention involves covering the sensor with a gas permeable membrane to prevent naturally fluorescing substances in the blood from interacting with the fluorescent measurement.
  • a bodily fluid culture sensor is comprised of a pH sensitive absorbance based dye encapsulated in or isolated by a polymeric gas permeable, but proton impermeable matrix 4 and a fluorescent dye in a second polymeric matrix 2.
  • Reflective surface 6 can be included to facilitate the transmission of light to the detecting element 12.
  • interrogation light enters the sensor and is regulated by pH sensitive matrix 2 which in turn causes a change in ' the fluorescence emission 10 of the fluorophore in .matrix 4. This sensor offers the advantage of maximal surface.area.
  • a measurement is taken by first reading reference light intensity. Next the reading from the sensor disk is measured. The data is plotted by taking the ratio of reference, excitation light, to sample. In particular, as C0 2 levels increase in the blood collection bag, the absorbance of the absorbance dye changes, thereby changing the amount of light reaching the fluorescence layer and/or photodetector. This causes a change in emitted fluorescence that is detected.
  • the following examples serve to illustrate the method of the present invention. The concentration of reagents and other variable parameters are only shown to exemplify the methods of the present invention and are not to be considered limitation thereof.
  • an additional amount (10ml) is collected in such a manner as to be subsequently sectioned off from the unit to be transfused.
  • This additional blood is then expressed through a tube into a flexible bag, attached to the blood collection bag.
  • This side bag is supplied containing a microorganism growth media and an attached multi-layer sensor.
  • the sensor is capable of detecting microorganism growth by measuring an increase in C0 2 production through a change in fluorescence emitted from the sensor.
  • an initial reading of the - sensor is made using a portable handheld fluorometer / to produce a baseline fluorescence level. This level can be manually , recorded for latter comparison or a bar code can be provided and attached to the bag.
  • the blood and additional monitoring bag are then stored in a normal manner (4°C) .
  • a second reading of the sensor is made and compared to the first reading. This is compared to the original reading. If a bar code was produced, the bar code and sensor are read.
  • the instrument will compare the initial and final fluorescent values and based upon an established threshold level of change will signal negative or positive for growth. In this particular embodiment, the instrument will signal green for no growth or red for growth based on differences in original and final sensor readings.
  • Wacker silicone elastomer 3601 part A is thoroughly mixed with Wacker 3601 catalyst part B in a 9:1 ratio, as recommended by the manufacturer.
  • the absorbance layer mixture is then poured into an aluminum square mold to a thickness of 30/1000 of an inch and cured at 55"C for 2 hours.
  • Wacker silicone is prepared, as described above. Next 2% w/w of 7.5mM Rhodamine 101, in 50mM Tris-HCl buffer pH 8.5 in 95% ethylene glycol, is added to the silicone. The mixture is poured over the previously cured xylenol blue layer in the mold, - described above, and cured at 55°C overnight.
  • This __ured, dehydrated, double layer sensor consists of two distinct layers., each 30/1000 of an inch thick. Disks may now be punched out of the mold and adhered onto the base of bottles using more silicone, ensuring that the absorbance layer is face down.
  • bottles are cured at 55°C for 15 minutes, rehydrated with normal saline and autoclaved on the wet cycle for 17 minutes.
  • Saline is replaced with growth media and inoculated with E. coli by injecting a suspension with a sterile needle through the septum.
  • the blood culture bottle is placed in the instrument and fluorescence emission is measured.
  • the pH sensitive absorbance dye, Xylenol blue As the concentration of C0 2 increases in the blood culture bottle, the pH sensitive absorbance dye, Xylenol blue, the absorbance of the dye decreases, thus allowing more light to reach the fluorophore, Rhodamine 101, to thus increase the amount of fluorescence emitted at 590nM. This increase in fluorescence intensity v. time is shown in the blood culture growth curve at Fig. 2.
  • Rhone Poulenc silicone elastomer 141 part A is thoroughly mixed with Rhone Poulenc 141 catalyst part B in a 10:1 ratio, as recommended by the manufacturer.
  • the absorbance layer mixture is then poured into an aluminum square mold to a thickness of 30/1000 of an inch. The mold is allowed to sit out on the countertop at room temperature for about one hour or until the bubbles have disappeared, at which time the mold is placed in the incubator to cure at 55°C for two hours.
  • Rhone-Poulenc silicone is prepared, as described above. Next, a 40/1,000" thick acrylic disc (Glasflex, Inc.), approximately 1 cm in diameter, containing 0.2 grams/lb of rhodamine B (Sigma) is glued onto the above absorbance layer using the Rhone-Poulenc Silicone at the 10:1 ratio as glue. The double layer sensor is then placed back in the 55°C incubator for two hours to allow for adherence of the two layers. Following the curing, the double layer sensor is punched out with a cork borer, and glued onto the base of a Wheaton bottle, ensuring that the absorbance layer is face down, using the Rhone Poulenc silicone as mentioned above. The bottle is placed in the 55°C incubator to cure for at least two hours. The bottle is then rehydrated overnite and tested the following day as described in Example 1.
  • Example 4 Xylenol Blue in Silicone/6213 Red Standard Acrylic Wacker silicone elastomer 3601 part A is thoroughly mixed with Wacker 3601 catalyst part B in a 9:1 ratio, as recommended with Wacker 3601 catalyst - part B in a 9:1 ratio, as recommended by the manufacturer. Next 5% w/w of a 50mM xylenol blue, dissolved in 5mM borate buffer pH 11 containing 1%
  • Tween 80 is added to the silicone and homogenized to ensure a uniform distribution of the dye.
  • the absorbance layer mixture is then poured into an aluminum square mold to a thickness of 30/1000 of an inch and cured at 55"C for two hours.
  • a 40/1,000" thick acrylic disc (Glasflex, Inc.) , approximately 1 cm in diameter, referred to as No. 6213 Red (Glasfle:* Standard Product) is glued onto the above absorbance layer using the Wacker silicone at the 9:1 ratio as glue.
  • the double layer sensor is then placed back in the 55"C incubator for two hours to allow for adherence of the two layers.
  • the double layer sensor is punched out with a cork borer, and glued onto the base of a Wheaton bottle, ensuring that the absorbance layer is face down, using the Rhone Poulenc silicone as mentioned above.
  • the bottle is placed in the 55°C incubator to cure for at least two hours.
  • the bottle is then rehydrated overnite and tested the following day as described in Example 1.
  • Wacker silicone elastomer 3601 part A is thoroughly mixed with Wacker 3601 catalyst part B in a 9:1 ratio, as recommended by the manufacturer.
  • 5% w/w of 50mM bromthymol blue, dissolved in 5mM tris buffer pH 12 in ethylene glycol, is added to the silicone and homogenized to ensure a uniform distribution of the dye.
  • the absorbance layer mixture is then poured into an aluminum square mold to a thickness of 30/1000 of an inch and cured at 55°C for two hours.
  • Wacker silicone is prepared, as described above.
  • 2% w/w of 7.5mM Rhodamine 101 in 50mM Tris-HCl buffer pH 8.5 in 95% ethylene glycol, is added to the silicone.
  • the mixture is poured over the previously cured xylenol blue layer in the mold, described above to isolate the absorbance layer.
  • This sensor is then cured at 55°C overnight.
  • This cured, dehydrated, double layer sensor consists of two distinct layers, each 30/1000 of an inch thick. Disks may now be punched out of the mold and adhered onto the base of bottles using more silicone, ensuring that the absorbance layer is face down.
  • the bottles are cured at 55"C for 15 minutes, rehydrated with normal saline and autoclaved on the wet cycle for 17 minutes. Saline is replaced with growth media and inoculated with E. coli by injecting a suspension with a sterile needle through the septum.
  • the blood culture bottle is placed in the instrument and fluorescence emission is measured. The increase in fluorescence intensity v. time is shown in blood culture growth curve in Fig. 5.

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Abstract

Cette invention concerne un appareil surveillant la croissance des fluides corporels collectés destinés à la transfusion. Plus particulièrement, cet appareil comprend un sachet souple (20) contenant du sang prélevé ou un sachet d'échantillon (2) contenant un milieu de croissance microbien. Un capteur (22) fixé sur la paroi interne du sachet (20) sert à détecter de manière non invasive la contamination microbienne à l'intérieur dudit sachet (20). Cette invention concerne également un procédé permettant de détecter la croissance microbienne dans un sachet contenant (20) du sang prélevé, juste avant d'effectuer une transfusion.
EP92911615A 1991-05-08 1992-04-29 Procede et appareil permettant de detecter la contamination bacterienne du sang destine aux transfusions Withdrawn EP0538450A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69708091A 1991-05-08 1991-05-08
US697080 1991-05-08

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EP0538450A1 true EP0538450A1 (fr) 1993-04-28
EP0538450A4 EP0538450A4 (fr) 1994-04-06

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EP92911615A Withdrawn EP0538450A1 (fr) 1991-05-08 1992-04-29 Procede et appareil permettant de detecter la contamination bacterienne du sang destine aux transfusions

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EP (1) EP0538450A1 (fr)
JP (1) JPH05508556A (fr)
AU (1) AU1915892A (fr)
CA (1) CA2086608A1 (fr)
WO (1) WO1992019764A1 (fr)

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US5843699A (en) * 1997-04-08 1998-12-01 Difco Laboratories, Inc. Rapid microorganism detection method
GB2339903A (en) * 1998-07-23 2000-02-09 Fsm Technologies Ltd Fluid container
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WO1992019764A1 (fr) 1992-11-12
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CA2086608A1 (fr) 1992-11-09
JPH05508556A (ja) 1993-12-02

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