EP0531387A1

EP0531387A1 - A method of reducing the irritating properties of a cosmetic composition.

Info

Publication number: EP0531387A1
Application number: EP91910181A
Authority: EP
Inventors: Kirsti Maarit Jutila
Original assignee: Cultor Oyj
Current assignee: Finnfeeds Finland Ltd
Priority date: 1990-06-05
Filing date: 1991-06-05
Publication date: 1993-03-17
Anticipated expiration: 2011-06-05
Also published as: ES2093102T3; EP0531387B1; DE69121405D1; DE69121405T2; ATE141154T1; WO1991018588A1

Abstract

A method for substantially reducing skin irritation and improving moisturizing properties of cosmetics by incorporating a trimethylglycine compound in an effective amount, and cosmetic products produced thereby are disclosed.

Description

A METHOD OF REDUCING THE IRRITATING PROPERTIES OF A COSMETIC COMPOSITION

BACKGROUND OF THE INVENTION

There is an increasing demand for better and more efficient raw materials for cosmetics and related products. Even the most efficient raw materials can be very irritating or sensitizing for human skin, thus preventing their regular use.

Some of the active compounds in creams, decora¬ tive cosmetics, etc., may cause irritation or skin-sen- sitization. Such active ingredients may include emol¬ lients, barrier agents, healing agents, humectants, emulsifiers, preservatives, perfume oils, coloring agents, and/or surfactants, particularly anionic surfac¬ tants (e.g., sodium lauryl ether sulphates), and several compounds for skin cleansing (which contain kerosenεes, solvents and disinfectants in addition to hard surfac- tants) . Other cosmetics such as deodorants containing ethanol are recognized as having potentially irritating or sensitizing properties. In addition, this problem is often found with many other medicated products.

Cationic compounds such as cetyltrimethyla - monium chloride, alkyldimethylbenzylammonium, alkyliso- quinidinium, and alkylpyridinium halides are commonly used as hair conditioners. In small amounts, these quaternary ammonium derivatives improve hair manage¬ ability. One drawback of including quaternary ammonium compounds in cosmetics such as shampoos, hair condi¬ tioners, creams, lotions, etc. is the fact that great care must be taken when introducing them into commercial products, as most of these materials are known to be skin irritants. More particularly, it is known from the literature that the quaternary compounds of synthetic origin, particularly at higher concentrations (over 10 percent) , cause skin irritation and cauterization.

This problem is overcome in hair conditioners and hair rinses because these preparations are diluted about 15 times with water to form the actual rinse used on the hair. Thus, in general, it has been found that when quaternary compounds are present in concentrations upon dilution with water of 0.1 percent, they are usually still safe and effective on the hair shaft. Certain other cationic compounds introduced into conditioners have been touted as having a lower level of irritancy than earlier compounds. Examples of such include ethoxylated quaternary ammonium phosphates, quaternized fatty acid amino-amides derived from lanolic acids and mink oil, and N-acyl colaminoformylmethylpyri- dinium derivatives.

Quaternary ammonium salts have also previously been used at concentrations below 0.1 percent as anti¬ septics in preparations such as aftershave lotions and baby lotions and creams.

One hair treatment composition, described in U.S. Patent No. 4,752,467 (Konrad et al.), contains 0.1-25 percent by weight betaine and 0.1-10 percent by weight of an aliphatic organic acid such as citric acid. The combination of the betaine/aliphatic organic acid is said to provide synergistic conditioning properties which improve the condition of the hair structure, and act as an antioxidant and as a buffer.

DE-Patent No. 1911144 (Medisan Ab) describes a skin-treating composition for treating abnormal skin conditions or as a vehicle for other medicaments, com¬ prising an aqueous phase in which urea and lactic acid are dissolved. The mode of operation of this composition relates to the skin-softening properties of urea and the keratolytic effect of lactic acid. Optional components of this composition include emulsifiers and a ino acid derivatives such as betaine.

Other cosmetic formulations also include betai¬ ne. Betaine is a general name for an organic tertiary amine with three free organic radicals. Known formula¬ tions include betaines in which at least one of the free radicals is a long fatty acid derivative based radical. U.S. Patent No. 4,490,355 (Desai) describes a mixture of cocoamidopropyl betaine and oleomidopropyl betaine which when included in cosmetics is said to improve thickening and foam boosting properties. U.S. Patent No. 4,654,161 (Kollmeier et al.) describes the use of organopolysila- xanes that have betaine groups in hair cosmetics. The use of these siloxane derivatives is said to provide improved compatibility with anionic additives (surfac¬ tants) and less irritation than prior organopolysiloxanes with quaternary ammonium groups. EP-Patent No. 286261 (Redken Laboratories) describes the use of zwitterions such as taurines and betaines to increase the substantivity of hydrolysed proteins in keratinous tissues.

It is an object of the present invention to provide a method of reducing the skin irritating proper¬ ties of a cosmetic composition containing substantially no hydrolysed protein.

It is another object of this invention to provide a cosmetic composition which has improved skin compatibility and moisturizing properties.

SUMMARY OF THE INVENTION

It has now been surprisingly discovered that adding an effective amount of a quaternary ammonium like compound which does not have any skin irritating proper¬ ties to cosmetic compositions reduces the skin irritating properties of skin irritating cosmetics and improves their skin compatibility and moisturizing properties. The present invention also relates to the method of reducing the skin irritating properties of a cosmetic composition.

The quaternary ammonium compound, preferably obtained from natural sources, is ethanaminium, l-carboxy-N,N,N-trimethyl-,hydroxide inner salt monohydrate or the corresponding anhydride thereof (also referred to as trimethylglycine or trimethylglycine anhydride respectively) .

Tήe present invention also relates to a cosmetic composition comprising one or more cosmetic ingredients selected from the group consisting of an active agent, an emollient, a barrier agent, a healing agent, a humectant, an emulsifier, a preservative, a perfume oil, a coloring agent, a deodorizing agent, a surfactant, a solvent and mixtures of the above and one or more of the cosmetic ingredient having irritating properties when applied to human skin, and trimethylglycine or anhydride in an amount effective to substantially neutralize the skin-irritating properties of the cosmetic ingredient. The dosage of trimethylglycine (or its anhydride) is from about 0.1 to about 50, preferably 1 - 15, percent by weight.

The cosmetic compositions of the present inven¬ tion include skin cleansers, body shampoos, hair condi- tioners, skin creams or lotions, deodorants, etc.

DETAILED DESCRIPTION The addition of trimethylglycine, a natural quaternary ammonium like compound methanaminium, l-carboxy-N,N,N-trimethyl-,hydroxide inner salt monohyd¬ rate or the corresponding anhydride (trimethylglycine/ah)

CH.

CH₃ N CH₂ COO- .H₂0

CH₃ has now surprisingly been found to reduce the skin irritation properties or sensitisation reactions of cosmetic compounds including irritating ingredients such as sodium lauryl ether sulphate, and other anionic surfactants, cetyltrimethylammonium chloride, alkyl¬ dimethylbenzylammonium, and alkylisoquinidinium, alkylpyridinium halides, solvents etc. Trimethylglycine (or anhydride thereof) in question has been found to improve the skin compatibility and moisturizing characteristics of cosmetic compositions. Problems mentioned above are often found in products containing irritants, including skin and hand cleaners, skin lotions, skin creams, as well as medicated products. The dosage of methanaminium, l-carboxy-N,N,N-trimethyl-, hydroxide, inner salt monohydrate or corresponding anhydride is between 0,1 and 50%, preferably 1 - 15 w/w.

The invention is further elucidated by the following examples, which are not meant to limit the claims.

Examples 1-16

The effect of methanaminium, 1-carboxy- N,N,N-trimethyl,-hydroxide, inner salt monohydrate on skin compatibility and irritation were tested by human patch testing.

The patch testing was conducted as follows. In each of these Examples, the test materials were applied on the upper outer arm and the application arm was occluded with a patch for an initial contact period of 24 hours. The patches were then removed and the skin reaction was assessed using a numerical scoring system one hour later.

In order to assess the degree of irritancy, several signs symptomatic of skin irritation and their severity were noted using the scoring system below: 0 = no visible relevant reaction

1 = reaction just present

2 = slight reaction

3 = moderate reaction

4 = severe reaction The signs of irritation were awarded different ratings of significance as follows:

RATING 5 4

3 2 1 1 1

In order to obtain a numerical value for the total reaction at each site the severity score for each sign was multiplied by the rating. The resulting values were summed to give a total score for the degree of irritation at that site.

For example, the total score for a site assessed as R3 , D2 and Gl would be:

( 3 X 3 ) + ( 2 X 1 ) + ( 1 X 1 ) = 12 The skin irritation scores for the test and control products were compared statistically using the binomial probability test.

Immediately after assessment an identical fresh patch was applied to the same skin site for a further 24 hours (for a total of 48 hours) and skin reactions were again examined one hour after removal. The materials tested and the mean skin irritation scores after 24 hours and after 48 hours were as follows: Skin Irritation

Example Material Tested 24 hr. 48 hr.

Deionized water (control) 1.28 0.96

Trimethylglycine 50% w/v 0.44 0.32 in deionized water

3 sodium lauryl ether sulphate (SLES) 10.3%

4 SLES 10.3% + trimethylglycine 7% 5 SLES 10.3% + trimethylglycine 5% 6 SLES 10.3% + trimethylglycine 3.5% 7 SLES 10.3% + trimethylglycine 2% 8 SLES 5.15% + trimethylglycine 3.5% 9 Deodorant (Control) 10 Deodorant + 5% trimethylglycine 11 Medical cleaner 12 Medical cleaner + 5% trimethylglycine 13 Eau de cologne 14 Eau de cologne + 5% trimethylglycine 15 After Shave Lotion (Control) 1.46 16 After Shave Lotion + 5% trimethylglycine 0.85

It can be seen from the foregoing numerical results that trimethylglycine significantly reduced the irritancy of the sample to the skin. Where a "—" is indicated in the chart above numerical results were not obtained.

Example 17

Transepidermal Water Loss fTEWL)

Irritants have much stronger effect on dry skin; thus ingredients of cosmetic compounds should not cause any increasing normal TEWL.

The effect of methanaminium, 1-carboxy- N,N,N-trimethyl-,hydroxide inner salt monohydrate on moisturizing and skin smoothness was tested by the tran- sepidermal water loss from skin (TEWL) . In example 17 a leading marketed skin cream was applied under an occlusive patch.

Skin Care Cream (commercially marketed) Ingredients

Glycerol monostearate 2.5

Cetyl stearyl ethoxylate 5.0

E ulsifier (Stearic acid) 2.0

White oil 25.0 Glycerol 10.0

Triethanolamine 0.8

Water Balance

The same cream containing 50% w/w trimethylglycine was applied under an occlusive patch at a different skin site. As a control, blank occlusive patches were also applied to a third skin site. The transepidermal water loss from skin (TEWL) was measured.

The test subjects were twelve female volunteers between 18 and 65 years of age. Panelists were selected on the + criteria that they had normal healthy skin on their forearms and were able to provide stable TEWL values.

Three sites were marked on the volar forearm of each volunteer at approximately equal intervals between the elbow crease and the wrist. The subject was then seated comfortably with her forearms exposed for a period of half an hour so that they would equilibrate with conditions of temperature and relative humidity in the laboratory.

TEWL readings were taken from each skin site to provide normal untreated water loss levels. Treatment was made via 12 mm occlusive Finn chambers. A blank Finn chamber served as the control site. The lotion samples were applied by immersing Whatman 3MM filter paper discs in the sample, removing excess product against the edge of the container and placing the disc in the Finn chamber using forceps. Each chamber was applied to one of the marked sites on the forearm and held in position with Scanpor non-occulsive surgical tape. The pattern of application of treatments to sites was randomized and recorded.

18 to 24 hours later each subject returned and was re-equilibrated as above. The chambers were then removed and the skin was blotted with tissue to absorb surface moisture. TEWL readings were recorded at 1 minute intervals from the time of removal of treatment until a steady valve was obtained, a period of ten to twenty-five minutes depending on the individual concerned and the treatment applied. Temperature and relative humidity were recorded at the start of each TEWL reading as gross changes can significantly alter the rate of water loss. The results for each panelist are presented graphically below. An analysis of the results revealed the following: 1) The 1 minute TEWL reading immediately after patch removal is lower for the cream containing trimet¬ hylglycine than for the cream alone indicating that the trimethylglycine-treated sites lost water less rapidly. The blank patch control is lower still and represents the skin hydrating effect of occlusion, without adding addi¬ tional moisture to the skin.

2) The final TEWL value obtained when readings approach a constant value is lower for the cream con¬ taining trimethylglycine than for the cream alone, and frequently also the blank control, again indicating better moisture retention at trimethylglycine-treated skin sites.

3) The rate at which the TEWL value decreases towards the normal untreated level is in general more rapid for the cream containing trimethylglycine than for the cream alone or the blank control.

These results are summarized in the table below: Initial TEWL level Final TEWL level Time for 75 % reduction

2 2

(g/m h) (g/m h) in TEWL rate (min)

Panel Blank Cream Cream Blank Cream Cream Blank Cream Cream

No. +TMG +TMG +TMG

1 ^"-* -58 «^■- 40 7

2 36 50 48 10 '

3 34 41 54 8

4 20 41 * 7

5 30 63 39 8

6 17 61 53 9

7 17 78 45 7

8 26 71 65 11

25 74 63

10 18 i 34 7

11 23 53 53 10

12 26 54 30 6

The "*" indicates that the evapormeter probe shifted over in the early part of curve so readings were not valid. The "i" indicates that the patch came loose over the application period. It can be seen that under these test conditions there were clear indications that the addition of trimet¬ hylglycine to the skin cream resulted in improved moisture retention when the product was applied to the skin.

Example 18

Example 18 provides illustrative formulations including ingredient with irritating properties for a skin cream, body shampoo and skin cleanser in accordance with the invention.

Skin cream Ingredient

Trioleyl phosphate Petrolatum

Glyceryl stearate

Isopropyl palmitate

Cetyl alcohol

Stearyl heptanoate (ir .) Cetearyl octanoate (irr.)

Sorbitol

Trimethylglycine

Water, perfume, preservative (irr.)

Shampoo

Ingredient

Sodium lauryl ether sulphate 30% (irr.)

Coconut monoethanolamide

Trimethylglycine Perfume, colour, water

HCl/NaOH

Sodium lauryl ether sulphate is known to have a hard cleansing effect; thus because of the side effect (e.g. too effective fat removal from the skin and hair) it cannot be used as such in shampoos. The addition of tri¬ methylglycine remarkably reduces the irritation effect.

Trimethyl glycine reduces the irritating properties of irritants.

Example 19 - Smoothness Test

Body Lotion With Improved Skin Compatibility Ingredient Amount (%) Emulsifier 2.0

Cetylalcohol (irr.) 2.5

Isopropylstearate 4.5

Preservative (potential irr.) 0.03 Water 78.77 1,2-Propyleneglycol 6.0

Trimethylglycine 0 or 6.0 Parfum oil 0.2

The effect of methanaminium, 1-carboxy-

N,N,N-trimethyl-,hydroxide inner salt monohydrate on skin tested by the Zeiss-spektrophotometer. This formula was tested by using ten female volunteers. The body lotion was used for 8 days. The smoothness of the skin was measured by a Zeiss-spektrophotometer by 660 nm. The results show that the addition of trimethylglycine moder¬ ately improves the smoothness of the skin. Smoothness of skin was calculated by the follow¬ ing equation:

= extinction after treatment _{χ QQ} initial extinction

Results for the ten subjects are reported below:

Age of the volunteer 0% 6% Trimethylglycine

43 44 51 50 70 47 34 64 30 51

Average 94.0 89.0

Example 20 - Moisture Holding Capacity Using Corneometer The same body lotion formula used in Example 19 was used in tests where the moisturizing effect of tri- methylglycine was measured by a Corneometer. The moisture holding capacity in the skin was increased when the trimethylglycine level in the product increased from 0% to 2% to 6%.

The same group of test persons and model products were used as in Example 19. Medial part of skin of arms were used (5 x 2.5 cm 2). Different test areas for dif¬ ferent model products were used. The instrument used for measurement of the lotions, skin moisturizing effect was a Corneometer Combi (Courage & Khazaka Electronic GmbH, FRG) . The subjects were acclimatized in a controlled atmosphere (vH 60 %, T° 24°C) for 30 minutes before 0-values of different skin areas were measured. Body lotions were used twice daily (12 hr. intervals) . Duration of the trials was 8 days.

The following are hydration measurements made by the Corneometer Combi:

Product Measurement after Minutes Ap l. 10 20 30 40 60 90 120

Control 107.8 106.3 106.8 107.8 107.7 108.7 107.6 108.2 107.8 0 % 104.5 155.8 118.5 118.4 116.1 115.7 113.2 112.6 111.8 2 % 104.0 152.9 120.0 119.2 115.9 117.1 113.8 113.8 112.8 6 % 103.6 150.4 122.1 118.7 116.1 117.6 116.1 116.3 114.9 trimethyl¬ glycine

Claims

PATENT CLAIMS:

1. A method of reducing the skin irritating properties of a cosmetic composition containing substan- 5 tially no hydrolyzed protein, c h a r a c t e r i z e d by the methanaminium,l-carboxy-N,N,N-trimethyl-, hydro¬ xide, inner salt monohydrate having the chemical struc¬ ture:

0

CH₃ 5 or an anhydride thereof is incorporated into the cosmetic composition in an amount effective to neutralize the skin-irritating properties of the cosmetic composition.

2. The method according to claim 1 0 c h a r a c t e r i z e d by that the methanami¬ nium,l-carboxy-N,N,N-trimethyl-, hydroxide, inner salt is added in concentration of 0,1 - 50%, preferably

0,1 - 15%, percent by weight.

3. A cosmetic composition having improved skin ^ compatibility and moisturizing properties containing substantially no hydrolysed protein and c h a r a c t e ¬ r i z e d by that it comprises a compound having irritating or sensitizing properties when applied to human skin, 0 an effective amount of a quaternary ammonium like compound, methanaminium,l-carboxy-N,N,N-trimethyl-, hydroxide, inner salt monohydrate having the chemical structure:

5 CH₃

I CH₃ N⁺ CH₂ COO- .H₂0

I CH₃

or anhydride thereof.

.

4.,,. The cosmetic composition of claim 3 or 4 c h a r a c t e r i z e d by that it is a skin cleanser, a skin cream, or body shampoo.

5. The cosmetic composition of claim 3 or 4 c h a r a c t e r i z e d by that the said compound is a deodorant.

The cosmetic composition of claim 3, wherein the concentration of methanaminium, l-carboxy-N,N,N-trimethyl-, hydroxide, inner salt monohydrate is from 0.1 to 50, preferably from about 0.1 to 15 percent by weight. The cosmetic composition of claim 3 or 4 c h a r a c t e r i z e d by that it is a cream, oint¬ ment, emulsion, or an aerosol.

6. A topical preparation according to claim 3 and having improved skin compatibility and moisturizing properties c h a r a c t e r i z e d by that it com¬ prises:

(a) one or more cosmetic ingredients selected from the group consisting of an active agent, an emollient, a barrier agent, a healing agent, a humectant, an emulsifier, a preservative, a perfume oil, a coloring agent, a deodorising agent, a surfactant, a solvent and mixtures of the above; and

(b) one or more cosmetic ingredients [may be included in group (a) and] having irritating properties when applied to human skin; and

(c) trimethylglycine or anhydride thereof in an amount effective to neutralize the skin-irritating effects of said cosmetic ingredient.