EP0530186B1 - Porte-objets pour preparations a teneur en eau, par exemple, de sang - Google Patents

Porte-objets pour preparations a teneur en eau, par exemple, de sang Download PDF

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Publication number
EP0530186B1
EP0530186B1 EP90916289A EP90916289A EP0530186B1 EP 0530186 B1 EP0530186 B1 EP 0530186B1 EP 90916289 A EP90916289 A EP 90916289A EP 90916289 A EP90916289 A EP 90916289A EP 0530186 B1 EP0530186 B1 EP 0530186B1
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EP
European Patent Office
Prior art keywords
plate
plates
frame
specimen slide
strip
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EP90916289A
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German (de)
English (en)
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EP0530186A1 (fr
Inventor
Rainer Dylla
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Individual
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Individual
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures

Definitions

  • the invention relates to a slide for wet or moist preparations, consisting of a plate made of preferably transparent material, in particular made of transparent plastic, with a plurality of receptacles for the preparations carried by the surface, a second plate which can be placed on the first plate being provided Surface is designed so that it forms with the surface of the receptacles of the first plate in each case a flat or slightly curved, area-extending gap space with such a gap width that the volume of the preparation essentially fills the volume of the gap space, the slide being a locking device has, which is effective when used for the first time and thereby forms a lock against further use.
  • Slides of the type mentioned are already known from US-A-4721679. It is a plate of box-like construction with numerous depressions arranged at the bottom of the box for receiving test liquid, and another plate which is intended to cover the depressions, this plate having downwardly extending edges which have a flat surface which are equipped with a pressure sensitive adhesive.
  • this adhesive layer firmly connects the cover to the cover Test liquid-carrying box construction and prevents both a lateral displacement of the two plates to each other as well as a vertical movement and prevents the loss of test liquid from the individual recordings or the contamination of the content of one recording by the content of another recording. This also prevents infection of personnel due to leakage of the test liquid.
  • a disadvantage of the prior art is that the protective cover (second plate) which can be placed on one (first) plate is provided with an adhesive layer for the purpose of locking, which firmly connects the two plates to one another after the second plate has been placed on the first plate , so that both a vertical and a lateral movement of the two plates to each other is prevented.
  • a sample carrier for carrying out chemical analyzes which also consists of two plates, which can be placed congruently with one another, the places where increases when collapsing the two plates meet equally strong depressions , which are preferably made of elastic material.
  • the two plates can also be designed as a frame, but there is no provision for a frame to be designed such that the plates can be inserted into it.
  • This elastic material allows the plates to be moved vertically to one another within a certain distance.
  • the publication deals with the fact that the two plates can be formed identically.
  • a sample carrier is known from DD 224 410 A1 (or DE 35 18 047 A1), in which the aim is to enable the two plates making up the sample carrier to be produced as inexpensively as possible.
  • the focus of this document is to design the border of the sample carrier so that it is mutually aligned Such a position cause the elevations and depressions of the opposing plates to be exactly aligned.
  • DD 224 410 A1 reference is made to another publication, namely to DD 107 783, according to which the sample carrier consists of two plates, on which bodies for sealing the sample-bearing areas of the two plates and for their horizontal position and vertical fixation Distance limitation are located, these bodies are made of elastic material in order to be able to move the plates vertically within a certain distance.
  • a disadvantage is the inaccurate alignment due to the grooves and their complicated manufacture.
  • EP 0018435 B1 According to which a horizontal position fixation and vertical distance limitation of two sample plates is carried out by means of a special frame.
  • DD 224 410 A1 in all known technical solutions the sample carrier is formed from two non-identical plates, which according to this DD 224 410 A1 has the disadvantage that two different plates have to be produced, which is particularly the case with an injection molding technique Realization would cause additional costs for the second tool required. Even when using the sample holder in the laboratory, the handling of two different plates has a disadvantageous effect.
  • the material from which the panels are to be made is plexiglass according to DD 224 410 A1.
  • the offset arrangement enables the setting and adherence to a more precise, reproducible sample thickness and the production of optically transparent samples and sample carriers, which enables immediate observation and detection of the reactions between the sample and the test medium as well as their immediate photometric evaluation without the interposition of filter paper, as is the case with older state of the art was necessary.
  • the samples are applied to the designated areas of one plate and the test agents are placed on the remote areas of the other plate, the plates are brought together with their surfaces carrying the reagents and the samples with the test agent by repeated slight Changing the distance between the two plates mixed by hand. The short distance also provides protection against rapid evaporation of the samples.
  • the plates can also be in the form of a film, in which case spacers could then be omitted because the plates are flexible enough so that the areas carrying the samples can be moved against one another.
  • the evaluation can be carried out subjectively by visual observation or objectively with analysis measuring devices or analysis machines.
  • EP 0018435 B1 deals with a device for performing microanalyses and describes in this connection various prior art in detail.
  • the delimitation of the sample areas takes place here by the application of water-repellent substances in such a way that a certain number of circular areas remain free. Mixing is also achieved here by changing the distance between the plates.
  • the plate movement is carried out by a magnet and by springs, the effect of the magnet are opposite.
  • DE 31 07 964 A1 originates from the same inventor as this EP 0018435 B1 and in this respect represents a further development of EP 0018435 B1.
  • Figure 9 of DE 31 07 964 A1 shows frames in order to "microscope" on this sample plate. The frames are essentially used for fixation when optically examining the samples.
  • OE-PS 17 08 98 deals with a shaking frame and which can be regarded as the forerunner of microtiter technology.
  • DD 96 784 describes a method and a device for measuring enzymatic reactions using plates in which a greater number of depressions are provided for receiving test liquids, these areas being separated from one another by grooves in order to prevent the test liquid from overflowing from one test area to the other test area.
  • test methods described which (advantageously) use very small amounts of preparation and test agent and therefore enable a given number of test liquids and quantities of preparation (such as blood) to carry out a significantly higher number of tests than previous test methods, the risk is relatively high that only small amounts of foreign matter can deliberately or unintentionally falsify the test result.
  • residues of previous preparation and test liquids or test materials that could not be removed cleanly enough could falsify the test result of a subsequent test. It is also possible with relatively little effort to change the test result through deliberate manipulation.
  • the object of the invention is to improve the arrangement described above in such a way that it no longer has these disadvantages and, in particular, no test falsifications by test plates already used, which, for. B. were not cleaned sufficiently, results, and the test result can not be changed by manipulation, without this z. B. would be visible to a controller.
  • test materials do not get back into the free environment through washing processes and lead to contamination or even infection there.
  • the locking devices allow a limited movement of the plates perpendicular to the plane of the plate against spring force to change the gap volume, but no complete separation of the plates from each other, and thereby in that the locking devices are formed by latching devices formed by two opposing plates, and in that the plates are inseparably connected to one another by the latching devices.
  • the movement of the plates for generating the mixing process can be generated by pressure against spring force, which spring force can be supplied by means of flexible approaches protruding in one piece from the plate material, for example by means of projections which start from the plate or by, as in the prior art the plate edges the plate levels are kept at a certain distance from each other, which distance can be reduced by pressure on the plates.
  • This pressure can be exerted by an operator's hand, but also by mechanical means.
  • the receptacles can be designed differently, for example by means of column-like projections starting from a substrate.
  • the projections can have been created by making intersecting grooves in a plate. This plate would therefore be made of solid material.
  • the slide is to be designed as a disposable article, which is the aim here, however, it is more economical in terms of material consumption to form the receptacles of bulges pressed out from a base plate made of thin plastic material.
  • These bulges can be a concave, convex or flat surface for the Form the receptacle, while the second plate has correspondingly shaped bulges, which are also pressed out of a plate made of thin plastic material. If the bulges of the first plate are flat, it may be sufficient if the second plate has no bulges, but is a simple flat plate.
  • Such plates which preferably consist of transparent plastic (the one plate also consisting of opalised plastic, which sometimes has microscopic advantages), can also be designed in a simple manner so that the first and / or the second plate in the region of the (respective) receptacle forms lens-like thickening, so that even very small test quantities can be observed so well with the naked eye, for example, that an exact check of the test result is possible without the need to use a microscope.
  • the slide can preferably be designed in such a way that it can be inserted into a (reusable) frame.
  • the slide can then be made from even thinner material because the frame can take on the mechanical loads that occur when handling (e.g. transporting, centrifuging, etc.). This further reduces the material consumption for the (discarded) slide.
  • the frame can then be designed in such a way that it is able to hold several slides at the same time, in which case the slide only has as many exposures as, for example, require a normal blood test.
  • the frame in the form of a snap frame with an upper and a lower part, which two parts can be pivotally connected to one another at one edge by a joint.
  • Folding frames can then be provided for several adjacent receptacles for strip-shaped plates.
  • strip-shaped plates can then again each form a plurality of receiving surfaces arranged next to one another in the longitudinal direction of the strip, for preparation and / or test liquid or ready-applied test agent.
  • a second latching device could also be provided, so that two strip-shaped plates lying one above the other form a latching device on at least two opposite edges, such as their two narrow edges.
  • the one (for example lower) strip-shaped plate could form a flag-like approach on at least one of its narrow sides in order to provide a marking or labeling area, for example for the manufacturer's specification of a permanently applied test agent or for user labeling for specifying patient data or preparation data.
  • This known sequence of processes is improved according to the invention in that the plate (s) are / are arranged in a frame, that after step c) the gap volume is changed cyclically by moving the plates perpendicular to their planes, thereby mixing the preparation and mixing with the test agent, and that the cyclical changing of the gap volume takes place by moving the two frames towards each other.
  • the plates can also be arranged in a frame before step a).
  • the two interlocked panels can be removed from the frame and replaced with new panels.
  • the frame is a snap frame and the new plates can be used to lock the opened frame. Subsequently, sample or test liquids can be applied and then the snap frame can be folded together by locking the plates, whereupon mixing then takes place by moving the frame parts towards one another. Before or after the assessment of the frames, they are then opened again in order to examine and / or to mark and / or to mark and / or to replace the locked panels remaining in the one frame part with new panels.
  • the advantage of a frame is that it is possible to design the two plates so that only as many pictures are available as is usually the case for a test, e.g. B. blood test are necessary, e.g. B. 6, 8, 10 or 12 shots.
  • the recordings in a certain grid, for example in an orthogonal grid (in which the recordings are arranged in rows and columns), or in a polar coordinate grid (in which the recordings are in concentric circles with one another lie).
  • a certain grid for example in an orthogonal grid (in which the recordings are arranged in rows and columns), or in a polar coordinate grid (in which the recordings are in concentric circles with one another lie).
  • Another alternative would be a spiral arrangement.
  • This grid-like arrangement facilitates machine inspection by, for example, successive scanning by means of an automatic device.
  • a slide 10 for wet or moist preparations for. B. chemical or biological preparations, such as blood, for which a blood group determination is to be carried out, consisting of a plate 12 made of plastic, with a plurality of columnar projections 16 extending from the surface 14, each of which forms a receptacle 18 for the preparation, that can be dripped onto the flat surface of the receptacle 18 with conventional devices, for example with pipettes, as is also indicated schematically in FIG. 8.
  • a second plate 20 which can be placed on the first plate 12, the surface 22 of which is designed (namely flat overall here) in such a way that it each has a surface 24 with the surfaces 24 of the receptacles 18, which are also flat (here also flat), areally extending gap space 30 with such a gap width 26 that the volume of the preparation, which is designated 28 in FIG. 8, for example, essentially fills the gap space 30.
  • the (liquid) material is held in the gap space, even if the two plates are now handled in some form, for example placed in an evaluation device. Even when the two plates are held at an angle, the material thus remains in the area of the receiving space 18 and does not flow into the area of other recordings.
  • the embodiment according to FIG. 2 is particularly space-saving because empty spaces lying between the individual receiving surfaces 127 are small.
  • the arrangement is also particularly well suited to enable automatic scanning of various samples, for example in that an optical evaluation device 36 scans the individual recording areas 118 individually in rows or columns, as indicated, for example, by the directional arrows 38.
  • FIG. 9 a side view, for example, again shows plate 112 and plate 20, with a gap width 26 shown here in a greatly exaggerated manner.
  • This gap width can be defined, for example, by projections 42, which are preferably close to the edge of one or both Plates. If you now press the two plates against each other, the distance 26 can be reduced, for example due to the resilience of the plate material, which also means the gap volume the recordings 118 decreases. If you let go again, the distance increases and the gap volume increases.
  • the preparation liquid (possibly including the test liquid) is spread out in the gap space volume and contracted again, spread out again and contracted again, which causes an extraordinarily strong mixing action.
  • Appropriate stop devices for example also in the form of projections 42, can thereby ensure that the change in gap volume complies with a favorable measure, for example it will be favorable if the gap space volume can be increased and / or reduced by approximately a factor of 2 compared to the preparation volume.
  • an arrangement such. B. could serve as a hinge (142. Fig. 13) and the other as a hinged closure, and which are designed so that they allow a substantially parallel movement of the two plates to each other.
  • the configuration is such that the projecting lugs 242 can withdraw into corresponding recesses 243, so that the two plates can come directly to each other in their edge regions, in which position a preferred volume size for the gap volume could be provided.
  • guide devices e.g. pin 443 in FIG. 11
  • connecting devices e.g. connecting devices and the like.
  • ⁇ Conceivable between the two plates, also stackable arrangements, as it is described in more detail in the prior art, so that further explanations are omitted.
  • FIG. 1 and FIG. 2 may consist essentially of solid injection molded plastic, but may also be pourable and grindable from glass
  • FIG. 3 shows an embodiment in which the receptacles 218 are made of a thin plastic material existing base plate 212 pressed bulges are formed. This embodiment saves material and is particularly suitable as a disposable item.
  • the second plate 220 can in turn consist of a flat plate, as in FIG. 1 or FIG. 2, or, as shown in FIG. 3, of a plate which in turn has bulges but is congruent with the bulges of the plate 212, see right side of FIG. 3.
  • Such an embodiment can also be used in reverse, in which the plate 220 forms the receptacle (as a depression) and the plate 212 represents the second plate which forms the gap space 230 when placed on the first plate forms.
  • the construction shown has the advantage that a mutual guidance between the two plates automatically results in that the protruding expressions or bulges of the plate 212 are encompassed by the recessed expressions of the plate 220.
  • FIG. 4 On the left side of FIG. 3, the two plates are shown as solid plates, an embodiment which is also possible and of which a sectional view along lines IV-IV is shown in FIG. 4. 4 (or according to the left half of FIG. 3) has a preparation 28 and a test liquid separated from it dripped onto the receptacle 318 according to FIG. 4, and if necessary also one (or more) further test liquid 48 (see FIG. 8) could be dripped on, or a test preparation already manufactured by the manufacturer, e.g. B. is applied as a gelatin layer.
  • the arrangement according to the invention should (and can also according to the invention) be handled so that two or more than two liquid components are initially separated the receptacle 118 are dripped on, then the second plate is placed, the entire arrangement is brought under the observation device and only then is the mixing process triggered by moving the two plates toward one another and thus the reaction being initiated, the reaction process then being observable with continuous observation.
  • FIG. 5 shows an arrangement in which the gap space 330 is curved in a view similar to FIG. 3, the receiving surface being able to form a concave curvature or a convex curvature depending on the position of the two plates.
  • FIG. 6 shows an arrangement in which the one of the plates, namely the second plate 420, represents an arrangement known per se, which, however, is used here "inappropriately", namely in such a way that the receiving spaces are not used in this way, but rather the usually unused outer surfaces of the floor form the cover for the receptacle 418 for the preparation, that of a
  • the first plate 412 or 512 can be formed, the plate 512 forming a lens-like device in the area of the receptacle 418, by means of material thickening 48.
  • the advantage of this arrangement is that devices that are already commercially available can be used once for regular tests, in which the receiving space 50 is used with its large volume, as previously, or, as shown in FIG.
  • a part of a plate 12 can be seen in FIG. 14, with a projection 16 which builds on it and forms a receptacle 18.
  • a lateral or annular capillary-like depression or gap 56 or 156 arranged near the edge of the receiving surface 24 emanates from this receptacle, which may also have a different shape, but must be arranged in such a way that samples applied to the receiving surface 18 - or test material has the opportunity to penetrate to a small extent into this gap or this depression 56, 156 as a result of capillary action, in such a way that a remainder remains in this gap even when it dries out or is washed out. If the gap is appropriately fine and the capillary action is correspondingly large, this can easily be achieved.
  • the material 16 is preferably transparent, material that has entered the slit 56, even if it is also transparent and colorless, changes the refraction ratios within the slit 56 such that the presence of this material can be seen by the eye or optical devices. This is particularly true when the material adhering in the gap 56 is colored, such as blood, which causes a red marking in this gap 56 as a result of the red blood cells, so that it can be seen immediately that the projection 16 with its receptacle 18, for example, for Blood tests have already been used. With a larger number of such projections 16, as shown for example in FIG. 1, it can therefore be seen which of these several projections have already been used and should not be used again.
  • FIG. 15 shows a lower plate 712, which can be designed similarly to the plate 212 according to FIG. 3, and an upper plate 714, which can be configured quite analogously, that is to say again how the plate 212 according to FIG. 3 can be shaped.
  • the plate as shown in FIG. 15, has five receptacles 718 lying next to one another, each with a flat surface here, locking and / or guiding devices being carried at both ends of this strip-like structure, which on the one hand move the two up and down Allow plates 712, 714 similar to the type described in FIGS. 9 to 13, but at the same time lock the two plates 712, 714 together in such a way that they cannot subsequently be taken apart and used again.
  • These devices 742, 743 can be configured differently on the left and on the right side of the strip-like plate 712 and 714, for example, twice on one side and only one on the other side, as can be seen in FIG. 15 is, or also be designed differently in some other way, so as to ensure that the two plates 712, 714 can, if necessary, be placed one on top of the other in only one orientation.
  • the handling of the strips according to the invention can be simplified even further if the strip or plate, for example comprising several recordings, has another Has area that can be used for identification, for example to identify the patient belonging to the blood sample.
  • FIG. 15 can be seen in an enlarged partial sectional view in FIG. 16, a special embodiment of the locking and guide devices 742 and 743 being shown here:
  • the locking takes place here in that a U-shaped receiving space of plate 712 is provided with protrusions 68, which lie behind recesses 70, which is formed by an arrow-like protrusion 743 of plate 714.
  • This arrow-like projection tapers and can also have an incision 72, so that the penetration of this arrow-like projection 763 into the U-shaped space 742 is made possible.
  • the plates 712 and 714 can still have stiffeners at the edge, for example by flanging 74.
  • FIG. 17 In order to make the material of this disposable arrangement according to FIG. 15 even thinner and thereby reduce the use of material without making handling more difficult, a further embodiment in the form of a frame arrangement is conceivable according to FIG. 17, consisting of a base frame 76 and optionally a cover frame 78, the two frames 76, 78 being able to be connected by means of a hinge 80 if desired, so that the two frames 76, 78 can be opened and closed in a convenient manner.
  • the base frame 76 either forms receiving spaces 82, 84 and 86 for test plate arrangements of different sizes which are of the same design or, as here, also have different designs.
  • a frame 76 according to FIG. 17 without intermediate walls 88 would, for example, be suitable for receiving a test plate 12 according to FIG.
  • the frames 76, 78 are designed in such a way that the optical assessment can take place without hindrance, for which purpose the frames 76, 78 either consist of transparent material themselves again, or there are corresponding openings in the frame base 290 or cover base 94.
  • additional trough-like, elongated receiving spaces 84 can be provided next to one another, for example to accommodate strip-shaped plates or plate combinations, as are shown in FIG. 15. Again, one plate could be accommodated in frame 76, the other plate in frame 78.
  • the bottom 92 could also be designed by means of pin-like projections in such a way that such cups or bowls are nonetheless held in place without intermediate walls 88, while differently designed strip-shaped receptacles, for example the shape of FIG. 15, are also held by such projections 76 so that with a single frame structure according to FIG. 76 optionally large-area recordings, or strip-shaped recordings or individual cup-shaped or cup-shaped recordings can be securely held, while the upper frame 78 then has the matching recording devices, for example an individual part 620 according to FIG. 8, so that the sample 28 obtains the desired flat spread within a capillary space 30.
  • the lens-like device 54 could then also be carried by the frame 78.
  • the strap parts 76, 78 are reusable, even if the receptacles used therein, for example for blood samples, are disposable items according to FIG. 15.
  • FIGS. 18 to 23 show in various views and representations a possible practical embodiment of a slide which can be inserted in a frame.
  • 18A, 18B and 18C show in a top view, a side view and a view from the right a first (lower) strip-shaped plate 812, pressed from approx. 1 mm thick plastic material (such as polyamide), the strip 812 here being a total of 10 Adjacent receptacles 818 of square shape here, which are formed similarly to the embodiment according to FIGS. 15 and 16 by material pushed upwards.
  • the strip 812 has raised edges 875, 877, 879 which surround the receiving surfaces 818 in a box-like manner.
  • Flag-like projections 881, 883 also emanate from the end face edge regions 877 and 879, which on the one hand facilitate handling, and on the other hand can serve as a marking area for patient data, sample data, and also for the dispensing of any test chemicals that may be present. From these edges 877, 879, lugs 885, 887 protrude inwards, with which lugs corresponding lugs 889, 890 of the second (upper) plate or strip shown in FIGS. 19A, 19B and 19C can hook, so that the two strips to lock together in their superimposed position. In the locked position, the receiving surfaces 814 of the strip 812 are aligned with the corresponding receiving surfaces 818 of the strip 814.
  • the strip 818 which also has a box-like stiffening by the edges 891, 893, 895, also this box shape can be inserted into the box formed by the strip 812.
  • approaches 882, 884 are present, which on the one hand serve as alignment and locking means when inserting this strip into the corresponding frame 878 according to FIG. 21A, see the depressions 982 there, but on the other hand also serve for marking purposes in the manner already described can.
  • the strip 814 serves a resiliently attached nose 896 on the two ends of the strip 814, which lies with its surface 897 behind a corresponding surface 997 of a projecting edge 996 of the frame 878 and thereby after the Inserting the strip into the two recesses 982 of the frame 878 and locking the strip by means of these lugs 296 behind the strip 996, the strip is firmly connected to the frame and can no longer fall out, even if, for example, this upper frame is folded down as it is can be seen, for example, in FIG. 22B.
  • the frame consists essentially of solid material, preferably again plastic, which can also be transparent, the construction details being shown in the figures.
  • Strips and frames are each constructed so that they cannot be mixed up, i.e. that is, a bottom strip cannot be placed in the top frame.
  • a depression 1082 is provided in the lower frame, see Figs. 20A-D, which is wide enough to accommodate the corresponding tab-like extension 883 of the lower strip 812, while the corresponding extension 884 of the upper Stripe 814 does not fit here and is therefore immediately recognizable that the wrong strip is inserted. This also applies conversely to the lower frame, in which only the lower strip fits, while the upper strip cannot be inserted here.
  • the lower strip 812 also has a locking projection 898 in order to fix the strip in the lower frame accordingly, for which purpose it has a projecting strip 1096 with a lower contact surface 1097, see FIG. 20B.
  • the lower strip protrusion 898 is shaped differently from the corresponding one Projection 896 of the upper strip, the design being selected such that the holding force in the frame for the lower strip is slightly less than the holding force of the upper strip within the upper frame.
  • the lower strip is placed in the lower frame, the strip being jammed in the frame with the aid of the projections 898 to such an extent that it can no longer fall out of its own accord, and then the upper strip 814 becomes in the upper one
  • the frame is inserted at the corresponding point aligned with the lower strip and clamped by means of the projection 896, it being possible for the frame 876 or 878 to lie flat on the work surface in this insertion position according to FIG. 22a.
  • the samples are then applied to the receptacles 818 of, for example, the lower strip, and a test liquid is applied to the corresponding receptacles on the upper strip, if this does not already contain a factory-applied test substance, whereupon the upper frame is placed on the lower frame ( 22B), as a result of which the two strips with their receptacles 818 come together and interlock and lock with one another with their projections 887 and 890.
  • the sample substance and test substance react with one another and give, for example, a precipitate that is visible through the transparent strip material surface.
  • the upper frame is removed from the lower frame again, for example by opening it again, whereupon the interlocked strips come loose from one of the frames, for example here from the lower frame, so that the interlocked strips remain in the upper frame and either with this to z. B. Control purposes are kept or removed from them and disposed of.
  • FIG. 23A shows a frame consisting of the frame parts 876 and 878 with a joint 880, the frame part 876 at its end carrying the joint 880 merging into an attachment 802 which is U-shaped in cross section and which opens at its open end is directed to the outer surface 803 of the frame part 876 and, with its closed end 804, projects beyond the inner surface 805 receiving the test strips to form a pivot bearing for the other frame part 878.
  • a bearing pin or pin 806 which can be slidably and rotatably received and which is fastened at both ends to a retaining tab 807 or merges into one which in turn laterally adjoins the (upper according to FIG 23B) frame part 878 is fastened, for example screwed by means of screws 808.
  • the bearing pin 806 has a radial depression 809 near each of its two ends (there may be further recesses in between), which is oriented such that it points towards the open end of the U when the frame is closed and towards a pin or a screw 811 is aligned, which in the position shown in FIG.
  • the bearing pin 806 is held in the position shown by a spring element 813, which is present, for example, in the form of a spiral spring placed around the pin 811, which is supported on the one hand on a locking strip 815 for the U-profile, on the other hand on the peripheral surface of the bearing pin 806 or on an intermediate piece slidably placed on this peripheral surface, not shown.
  • a spring element 813 which is present, for example, in the form of a spiral spring placed around the pin 811, which is supported on the one hand on a locking strip 815 for the U-profile, on the other hand on the peripheral surface of the bearing pin 806 or on an intermediate piece slidably placed on this peripheral surface, not shown.
  • This movement is used for mixing, for example, as already described.
  • the movement path of the two plates from one another and from one another is limited, for example in the present case by striking the shaft 806 on the bottom of the U-profile 802 ( Movement of the frame parts away from each other), on the other hand by the provision of stops between the two surfaces 805 and 817 of the frame parts facing each other in the closed position.
  • stops can be formed by the surfaces 805, 817 themselves, or by additionally attached stop projections or tabs 819 on one or both opposite surfaces 805, 817 of the frame parts 876, 878.
  • the construction described allows a stable guidance and alignment of the two frame parts 876, 878 to one another, so that it is ensured that, even in the case of very small receiving areas, the preparation liquid (on e.g. the lower frame part) and the test liquid (on z. B. the upper frame part) is ensured.
  • the design also ensures that a uniform parallel guidance is achieved when the two frame parts move with respect to one another, and that an identical stroke is achieved with all sample strips and thus also with all opposing individual samples, so that the mixing processes are the same for all samples are and z. B. meet a certain standard, so that it is ensured that different results do not result from different mixing intensities, which do not come about due to differences in preparation.
  • the construction described is also designed in such a way that it does not lead to canting when folded, since the compression of the two frame parts in the direction of arrows 823 is only possible when the two frame parts have reached the closed position, as shown in FIG. 23B .
  • misalignment of bore 809 with pin 811 prevents shaft 806 from escaping the U-shaped end of part 802.
  • the arrangement of a frame in which a large number of individual sample strips, each with a larger number of individual samples in turn, can be accommodated, also enables automation which is expedient in many cases.
  • the frame could be placed in a device that accomplishes the mixing process by, for example by magnetic devices or by pneumatic drive, the lifting movement of the two frame parts is forced towards each other, for example at a frequency of two lifting movements per second, so that at a stroke rate of about 50 to 60 for a thorough mix the total treatment time would be about 1/2 minute.
  • a mechanical drive would also enable an even more uniform application of pressure, although an arrangement of compression spring devices 821 in the area of the stops 819, for example, could also remedy this.
  • Such spring devices 821 could, for example, be attached to all four corners of the frame. If their compressive force does not significantly exceed that of the spring 813, so that the influence of the spring 813 is not negligible, such springs could only be arranged at the end facing away from the joint, in particular this applies if their compressive force would correspond approximately to that of the springs 813, so that ultimately a uniform distribution of the back pressure exerted by the springs at the four corners of the frame parts and then the overall springs would result.
  • This uniform pressure at all four corners of the frame facilitates a uniform movement of the frame parts 876, 878 towards one another if this is carried out by hand, whereas in the case of mechanical operation, corresponding devices can be provided in the drive device which automatically do this. So could be by appropriate locking devices (for example, by suction cups or jaws) between z. B. the drive jaws of a vibrator drive and the externally accessible surfaces of the two frame parts 876, 878 can be achieved that they are forcibly moved with a certain stroke and a certain speed, regardless of the presence of the springs 813, 821.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un porte-objets (10) pour préparations à teneur en eau, par exemple des préparations chimiques ou biologiques, telles que des préparations de sang, comprenant une première plaque (12) en une matière transparente de préférence, notamment en matière plastique transparente, présentant plusieurs logements (18) pour les préparations portés par la surface (14), cependant qu'il est prévu une deuxième plaque (20) applicable sur la première plaque (12) et dont la surface (24) est configurée de telle façon qu'elle forme, avec la surface (22) des logements (18) de la première plaque (12), respectivement, une fente plane ou légèrement incurvée (30) s'étendant à la surface et ayant une largeur (26) telle que le volume de la préparation remplisse sensiblement le volume de ladite fente. Pour la caractérisation de l'utilisation en cours, les plaques présentent un dispositif de verrouillage, ou bien l'utilisation de la plaque est signalée par des marquages se formant en cours d'essai. On utilise à cet effet soit des dispositifs de verrouillage, soit des découpes capillaires (56, 156) s'étendant à partir de la surface des logements.

Claims (23)

  1. Porte-objets (10) pour des préparations humides ou mouillées, constitué par une plaque (12) en un matériau transparent, présentant plusieurs logements (18) pour les préparations et supportés par la surface (14), une deuxième plaque (20) étant prévue qui peut être placée sur la première (12) et dont la surface (24) est exécutée de telle manière qu'elle forme, avec la surface (22) des logements (18) de la première plaque une fente (30) plate ou légèrement incurvée et plane de telle manière que le volume de la préparation remplisse le volume de la fente, le porte-objets présentant un dispositif de verrouillage qui devient actif lors de la première utilisation et qui empêche ainsi une autre utilisation, caractérisé en ce que les dispositifs de verrouillage (742, 743) permettent un mouvement limité des plaques perpendiculairement par rapport au plan des plaques (712, 714) contre une force de ressort pour modifier le volume de la fente mais sans permettre une séparation complète des deux plaques (712, 714), en ce que les dispositifs de verrouillage sont formés par deux dispositifs d'encliquetage disposés sur deux plaques opposées (887, 885; 890, 889) et en ce que grâce aux dispositifs d'encliquetage, les plaques sont reliées sans pouvoir les séparer.
  2. Porte-objets selon la revendication 1, caractérisé en ce que la force de ressort est fournie par des embouts (142, 242) flexibles en saillie par rapport à la plaque et formés par le même matériau que les plaques.
  3. Porte-objets selon la revendication 1 ou 2, caractérisé en ce que les plaques (12, 20) peuvent être déplacées (44) perpendiculairement par rapport au plan des plaques de telle manière que le volume de la fente par rapport au volume de la préparation peut être au moins multiplié et/ou divisé par un facteur 2.
  4. Porte-objets selon une quelconque des revendications 1 à 3, caractérisé en ce que les logements (18) sont formés par des parties en saillie (16) en forme de colonne qui partent à partir d'un substrat (12).
  5. Porte-objets selon la revendication 4, caractérisé en ce que les parties en saillie sont formées par des rainures (32, 34) qui se croisent et situées dans la plaque (112).
  6. Porte-objets selon une quelconque des revendications 1 à 3, caractérisé en ce que les logements (118) sont formés par des bosses estampées à partir d'une plaque de base (212) en matériau synthétique.
  7. Porte-objets selon la revendication 6, caractérisé en ce que les bosses forment une surface concave, convexe ou plate pour former le logement et en ce que la deuxième plaque présente des bosses ayant une forme correspondante qui sont également formées par estampage à partir d'une deuxième plaque en matériau synthétique fin.
  8. Porte-objets selon la revendication 7, caractérisé en ce que la première et/ou la deuxième plaque présente un épaississement en forme de lentille (48; 148) au niveau du logement.
  9. Porte-objets selon une quelconque des revendications 1 à 8, caractérisé par un cadre (76, 78) dans lequel on peut placer les plaques (712, 714) de telle manière qu'on peut maintenir et déplacer les plaques l'une par rapport à l'autre à l'aide du cadre.
  10. Porte-objets selon la revendication 9, caractérisé en ce que le cadre est un cadre à charnière avec une partie supérieure et une partie inférieure (878, 876) qui sont reliées d'un côté l'une à l'autre de manière à pouvoir pivoter par une articulation (999) qui permet au moins en position fermée un mouvement limité des parties du cadre l'une par rapport à l'autre dans une direction perpendiculaire par rapport au plan du cadre.
  11. Porte-objets selon la revendication 10, caractérisé en ce que le cadre à charnière (878, 876) présente plusieurs logements (992; 1082) situés les uns à côté des autres pour des plaques (814, 812) en forme de latte.
  12. Porte-objets selon la revendication 11, caractérisé en ce que les plaques (812, 814) en forme de latte présentent plusieurs surfaces de logement (818) disposées les unes à côté des autres dans la direction longitudinale des lattes et destinées à recevoir la préparation et/ou le liquide d'analyse ou contenant un agent d'analyse prêt à l'emploi.
  13. Porte-objets selon la revendication 12, caractérisé en ce que deux plaques (12, 814) opposées en forme de latte sont reliées d'un côté, par exemple le côté étroit, l'une à l'autre à l'aide d'une articulation et en ce qu'elles présentent un dispositif d'encliquetage du côté opposé.
  14. Porte-objets selon la revendication 12, caractérisé en ce que deux plaques (812, 814) opposées en forme de latte présentent en au moins deux côtés opposés, tels que leurs côtés étroits, un dispositif d'encliquetage (887, 885; 890, 889).
  15. Porte-objets selon la revendication 13 ou 14, caractérisé en ce qu'au moins une plaque, par exemple la plaque (812) inférieure en forme de latte, présente en au moins un de ses côtés étroits un embout (883) en forme de drapeau qui forme d'un surface d'inscription ou de marquage, par exemple pour que le fabricant puisse inscrire l'agent d'analyse solide appliqué ou pour que l'utilisateur puisse inscrire le type de préparation ou des données concernant la préparation.
  16. Porte-objets selon la revendication 15, caractérisé en ce que l'autre plaque présente également un embout en forme de drapeau qui s'aligne sur celui de la première plaque et qui recouvre sa zone d'inscription.
  17. Porte-objets selon une quelconque des revendications 1 à 16, caractérisé en ce que le matériau transparent est un matériau synthétique.
  18. Utilisation d'un porte-objets selon une quelconque des revendications 1 à 17 pour l'analyse du sang.
  19. Procédé pour analyser des préparations disposées dans les logements d'un porte-objets selon une quelconque des revendications 1 à 17 en suivant le mode opératoire ci-dessus:
    a) introduction de la préparation dans ou sur le logement ou les logements de la première plaque (12),
    c) placement de la deuxième plaque (20) de manière à former le volume de la fente (30) et aspiration de la préparation et de l'agent d'analyse dans le volume de la fente (30);
    d) observation optique de la préparation à travers le matériau transparent de la première et/ou la deuxième plaque, les deux plaques étant verrouillées l'une avec l'autre de manière inamovible par les dispositifs d'encliquetage situés sur les plaques opposées pendant et après l'étape c) et les plaques (12, 20) étant disposées dans un cadre (76, 78), le volume de l'espace, après l'étape c), étant cycliquement modifié en déplaçant les plaques (12, 20) perpendiculairement par rapport à leurs plans pour provoquer le mélange de la préparation et le mélange avec l'agent d'analyse et la modification cyclique du volume de la fente étant obtenue par le déplacement des deux cadres (76, 78) l'un par rapport à l'autre.
  20. Procédé selon la revendication 19, caractérisé par une étape de procédé b):
    introduction de l'agent d'analyse dans ou sur les logements où se trouve la préparation, près de la préparation ou sur la préparation, dans la première plaque ou dans ou sur les logements correspondants de la deuxième plaque (20) selon l'étape a).
  21. Procédé selon une quelconque des revendications 19 ou 20, caractérisé en ce qu'on place les plaques (12; 20) dans les cadres (76, 78) avant l'étape a).
  22. Procédé selon une quelconque des revendications 19, 20 ou 21, caractérisé en ce qu'après l'étape d), les deux plaques (12, 20) verrouillées sont sorties des cadres (76, 78) et remplacées par des nouvelles plaques.
  23. Procédé selon la revendication 22, caractérisé en ce que le cadre est un cadre à charnière et en ce que les nouvelles plaques peuvent être bloquées dans le cadre ouvert, en ce qu'ensuite les échantillons ou les liquides d'analyse sont introduits et en ce que le cadre à charnière est fermé en verrouillant les plaques, en ce qu'on procède à un mélange en déplaçant les parties du cadre l'une sur l'autre, en ce que le cadre est à nouveau ouvert, avant ou après l'examination, pour examiner et/ou marquer les plaques verrouillées qui se trouvent dans une partie du cadre et/ou pour les remplacer par d'autres.
EP90916289A 1990-05-23 1990-11-12 Porte-objets pour preparations a teneur en eau, par exemple, de sang Expired - Lifetime EP0530186B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE9005871U 1990-05-23
DE9005871 1990-05-23
PCT/EP1990/001892 WO1991017832A1 (fr) 1990-05-23 1990-11-12 Porte-objets pour preparations a teneur en eau, par exemple, de sang

Publications (2)

Publication Number Publication Date
EP0530186A1 EP0530186A1 (fr) 1993-03-10
EP0530186B1 true EP0530186B1 (fr) 1995-08-30

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EP90916289A Expired - Lifetime EP0530186B1 (fr) 1990-05-23 1990-11-12 Porte-objets pour preparations a teneur en eau, par exemple, de sang

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EP (1) EP0530186B1 (fr)
AU (1) AU7042391A (fr)
DE (1) DE59009600D1 (fr)
WO (1) WO1991017832A1 (fr)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE9217130U1 (de) * 1992-12-08 1993-04-08 Astra Chemicals GmbH, 2000 Wedel Vorrichtung zur Durchführung kombinierter Ureasetests von Antrum/Korpus-Biopsien zur Diagnose von Gastrointestinalerkrankungen
DE4405375C2 (de) * 1994-02-19 1996-07-25 Fritz Nerbe Nachfolger Juergen Mikrotiterplatte
US5795748A (en) * 1996-09-26 1998-08-18 Becton Dickinson And Company DNA microwell device and method
DE19827754C1 (de) 1998-06-23 2000-02-10 Graffinity Pharm Design Gmbh Einrichtung für eine nahezu gleichzeitige Synthese einer Vielzahl von Proben
DE29907804U1 (de) * 1998-04-30 1999-10-07 Graffinity Pharmaceutical Design GmbH, 69120 Heidelberg Vorrichtung für den Transport von Flüssigkeiten entlang vorgegebener Leitwege
DE19907011A1 (de) * 1999-02-18 2000-08-31 Deutsches Krebsforsch Fluoreszenzkorrelationsspektroskopievorrichtung und -verfahren, insbesondere zur Mehrfarbenfluoreszenzkorrelationsspektroskopie

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0111762B1 (fr) * 1980-06-20 1987-11-19 Unilever Plc Procédés et appareil pour l'exécution d'essais de liaisons spécifiques
GB2147698B (en) * 1981-11-17 1986-05-08 Unilever Plc Test apparatus for immunoassay
DE3505892A1 (de) * 1985-02-20 1986-08-21 GVB SANIMED Hygiene- und Medizintechnik GmbH, 3070 Nienburg Verschliessbarer behaelter zur aufnahme von laborproben, sondermuell oder dergleichen
US4721679A (en) * 1986-10-23 1988-01-26 Yiu Felix H F Tissue typing tray

Also Published As

Publication number Publication date
WO1991017832A1 (fr) 1991-11-28
EP0530186A1 (fr) 1993-03-10
AU7042391A (en) 1991-12-10
DE59009600D1 (de) 1995-10-05

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