Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
  • A01N63/10—Animals; Substances produced thereby or obtained therefrom
  • A01N63/14—Insects

Definitions

• the invention relates to the purification and characterization of an anti-oviposition pheromone, as well as to a chemical composition having the anti-oviposition properties of said pheromone, and to the application of said pheromone and said compound. ⁇ the fight against insect pests.
• Anti-oviposition pheromones are intra-specific chemical signals deposited by certain female insects on and / or in the vicinity of their eggs after laying. The locations thus marked are then rejected by the other females of the same species as nesting sites.
• the other is the anti-oviposition pheromone of the dipteran Rhagoletis cerasi, which is a derivative of palmityl glucopyranoside; the synthesis of the four possible stereoisomers of this derivative has been carried out [ERNST and WAGNER, Helv. Chim. Acta, 22, 165-171, (1989)], in order to determine
• REPLACEMENT SHEET ner which corresponded to the active natural form of the pheromone.
• KLIJNSTRA Entomol. exp.appl., &, 139-146, (1986)
• pesticides essentially synthetic pyrethroids.
• these pyrethrinoids are increasingly suspected of promoting the spread of aphid populations, and of diseases, in particular viral, inoculated by sucking biting insects.
• the Inventor is the first to extract such a substance from the eggs of the corn borer. Analysis of the extract thus obtained revealed that it was a complex mixture, as shown by the chromatographies of Figures 1 and 2.
• the inventor has also managed to select and identify in this mixture, constituents which, surprisingly, have a anti-oviposition activity comparable to that of the total extract.
• the present invention relates to a prepa ⁇ ration of anti-oviposition pheromone, which preparation is characterized in that it is suscep ⁇ tible to be obtained from a methanolic extract of the eggs of the corn borer, and in what it includes a mixture of the methyl esters of palmitoleic, palmitic, linoleic, oleic and stearic acids.
• said preparation comprises: between 10 and 20 parts of methyl palmitoleate, between 10 and 20 parts of methyl palmitate, between 10 and 20 parts of methyl linoleate, between 10 and 20 parts methyl oleate, for 1 to 2 parts of methyl stearate.
• the identification of active components has enabled the formulation of simple compositions reproducing the effect, on the reduction of the laying of the corn borer, of the preparation of anti-oviposition pheromone in accordance with the invention.
• the present invention further relates to an anti-oviposition composition, active in particular on the corn borer, and characterized in that it comprises as active ingredient, at least one alkyl ester in and C-j_4_20 fatty acid *
• an anti-oviposition composition in accordance with the invention, it comprises, as active principle, at least one methyl ester of a fatty acid with a straight chain C- j ⁇ g-
• said methyl ester of fatty acid with a straight chain is chosen from the group which comprises: methyl palmitoleate, methyl palmitate, methyl linoleate, oleate of methyl, methyl stearate, and mixtures thereof.
• an anti-oviposition composition in accordance with the invention comprises: between 10 and 20 parts of methyl palmitoleate, between 10 and 20 parts of methyl palmitate, between 10 and 20 parts of methyl linoleate, between 10 and 20 parts of methyl oleate, for 1 to 2 parts of methyl stearate.
• said composition comprises at least 0.08 g / l of methyl palmitoleate, 0.1 g / l of ⁇ -methyl palmitate, 0.08 g / l of methyl linoleate, 0 , lg / l of methyl oleate, 0.008 g / 1 of methyl stearate
• a composition in accordance with the invention comprises: between 0.3 and 2 g / 1 of methyl palmitoleate, between 0.3 and 2 g / 1 of methyl palmitate, between 0.3 and 2 g / 1 of methyl linoleate, between 0.3 and 2 g / 1 of methyl oleate and between 0.03 and 0.2 g / 1 of methyl stearate.
• an anti-oviposition composition comprising 1.1 g / l of methyl palmitoleate, 1.45 g / l of methyl palmitate, 1.1 g / l of methyl linoleate, 1.3 g / 1 methyl oleate and 0.1 g / 1 methyl stearate, dissolved in a mixture comprising 1 volume of methanol for 9 volumes of water, can be used for the treatment of corn plants under the following conditions: - single application - spraying of the composition on one row of corn on two.
• 1'Invention can be obtained in a simple and inexpensive, from alkyl esters of fatty acids which are readily available commercially. They can therefore be used on a large scale in the treatment of crops.
• the present invention therefore also relates to the application of a composition, or a purified preparation of an anti-oviposition pheromone, as defined above, to the protection of plants against insect pests, particularly European moth. But.
• the subject of the present invention is a process for protecting plants against insect pests, particularly the European corn borer, which process is characterized in that the plants to be treated are brought into contact with a composition or a preparation of pheromone. anti-oviposition according to the invention.
• FIG. 1 represents the profile obtained on a CPWAX58 column.
• FIG. 2 represents the profile obtained on a DB5 column.
• the compounds thus identified by each of these methods were compared, on the basis of their retention time, to standards.
• the dosage of the constituents was obtained by gas chromatography.
• the selected components were identified by infrared spectrometry, coupled with FOURIER transform analysis
• Methyl palmitoleate, methyl palmitate, methyl linoleate, methyl oleate, and methyl stearate are mixed in the proportions indicated in Table I above. The mixture is dissolved in methanol EXAMPLE 4 - COMPARISON OF THE EFFICIENCY OF THE COMPOSITIONS
• Each oviposition substrate has been divided into two zones: one is impregnated with the test solution (egg extract prepared according to the procedure described in Example 1, composition prepared according to the procedure described in Example 3, or solvent alone) at the desired concentration, the other is not impregnated.
• the test solution egg extract prepared according to the procedure described in Example 1, composition prepared according to the procedure described in Example 3, or solvent alone
• the deposit is made using a precision syringe. 90 drops of 2.5 ⁇ l each are deposited on the test area.
• the concentrations used are expressed in opaque / drop equivalent, and are 0.7, 2, and 4.
• the anti-oviposition composition prepared according to the procedure described in Example 3 is used at a concentration of 1.616 g / ml of the mixture of methyl esters of fatty acids, which corresponds to 4 opaque equivalents / drop.
• the treated oviposition supports were placed in the cages. 2-day-old borer females were left in the presence of supports, for 2 consecutive nights, at the end of which the ooplaques deposited on the treated areas, on the one hand, and on the untreated control areas, d on the other hand, were enumerated.
• FIG. 3 represents the results obtained with the preparation of egg extract: on the ordinate, the number of opoplates deposited on the treated area ( as a% of the total number of opoplates deposited on the support), and, on the abscissa, the concentration of the extract in ooplate equivalents / drop.
• Figure 4 shows the comparison of the effectiveness of the preparation of egg extract of and the composition prepared as described in Example 3, on the reduction of the laying of the corn borer.

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• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Zoology (AREA)
• General Health & Medical Sciences (AREA)
• Health & Medical Sciences (AREA)
• Wood Science & Technology (AREA)
• Dentistry (AREA)
• Plant Pathology (AREA)
• Pest Control & Pesticides (AREA)
• Agronomy & Crop Science (AREA)
• Environmental Sciences (AREA)
• Insects & Arthropods (AREA)
• Biotechnology (AREA)
• Microbiology (AREA)
• Virology (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)
• Catching Or Destruction (AREA)

Applications Claiming Priority (2)

Publications (1)

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ID=9399610

Family Applications (1)

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• 1990
  • 1990-08-10 FR FR9010253A patent/FR2665610B1/fr not_active Expired - Fee Related
• 1991
  • 1991-08-02 EP EP91914378A patent/EP0495955A1/de not_active Withdrawn
  • 1991-08-02 CA CA002066589A patent/CA2066589A1/fr not_active Abandoned
  • 1991-08-02 JP JP3513664A patent/JPH05502045A/ja active Pending
  • 1991-08-02 WO PCT/FR1991/000641 patent/WO1992002138A1/fr not_active Application Discontinuation
  • 1991-08-06 IL IL99105A patent/IL99105A0/xx unknown

Non-Patent Citations (1)

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