EP0449839A1 - Improved bacterial strains for heterologous gene expression - Google Patents

Improved bacterial strains for heterologous gene expression

Info

Publication number
EP0449839A1
EP0449839A1 EP19890911317 EP89911317A EP0449839A1 EP 0449839 A1 EP0449839 A1 EP 0449839A1 EP 19890911317 EP19890911317 EP 19890911317 EP 89911317 A EP89911317 A EP 89911317A EP 0449839 A1 EP0449839 A1 EP 0449839A1
Authority
EP
European Patent Office
Prior art keywords
genes
gene
strain
bacterial
proteolysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19890911317
Other languages
German (de)
English (en)
French (fr)
Inventor
Ina Goldberg
Anthony Joseph Salerno
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Honeywell International Inc
Original Assignee
AlliedSignal Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AlliedSignal Inc filed Critical AlliedSignal Inc
Publication of EP0449839A1 publication Critical patent/EP0449839A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP19890911317 1988-09-30 1989-09-06 Improved bacterial strains for heterologous gene expression Withdrawn EP0449839A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25171488A 1988-09-30 1988-09-30
US251714 1988-09-30

Publications (1)

Publication Number Publication Date
EP0449839A1 true EP0449839A1 (en) 1991-10-09

Family

ID=22953092

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19890911317 Withdrawn EP0449839A1 (en) 1988-09-30 1989-09-06 Improved bacterial strains for heterologous gene expression

Country Status (3)

Country Link
EP (1) EP0449839A1 (ja)
JP (1) JPH04500754A (ja)
WO (1) WO1990003438A1 (ja)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0495638A3 (en) * 1991-01-16 1993-06-02 Schering Corporation Expression of mature pdgf-b in escherichia coli
US5200341A (en) * 1991-10-07 1993-04-06 Monsanto Company Altered gene and E. coli strains and methods useful in enhanced accumulation of proteins
FR2685347A1 (fr) * 1991-12-23 1993-06-25 Univ Pasteur Procede biotechnologique d'obtention et de production microbienne d'oligomeres peptidiques comme substituts de la gelatine.
CN1084889A (zh) * 1993-05-19 1994-04-06 中国科学院上海有机化学研究所 天花粉蛋白合成基因
US6927287B1 (en) 1996-06-03 2005-08-09 United States Surgical Corporation Nucleic acid encoding extracellular matrix protein or fragment thereof
US5821089A (en) * 1996-06-03 1998-10-13 Gruskin; Elliott A. Amino acid modified polypeptides
US6492508B1 (en) 1996-06-03 2002-12-10 United States Surgical Corp. A Division Of Tyco Healthcare Group Nucleic acids encoding extracellular matrix proteins
GB9803424D0 (en) * 1998-02-18 1998-04-15 Pfizer Ltd Performance enhancement
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US20040117874A1 (en) * 2002-10-15 2004-06-17 Jianjun Yang Methods for accumulating translocated proteins
CA2574953A1 (en) 2004-07-26 2006-02-09 Dow Global Technolgies Inc. Process for improved protein expression by strain engineering
CN101688213A (zh) 2007-04-27 2010-03-31 陶氏环球技术公司 用于快速筛选微生物宿主以鉴定某些在表达异源蛋白质方面具有改善的产量和/或质量的菌株的方法
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2068971B (en) * 1980-01-30 1983-06-08 Searle & Co Recombinant dna techniques
SE8505921D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab A method to export gene products to the growth medium of gram negative bacteria
WO1988003533A1 (en) * 1986-11-04 1988-05-19 Syntro Corporation Construction of synthetic dna and its use in large polypeptide synthesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9003438A1 *

Also Published As

Publication number Publication date
WO1990003438A1 (en) 1990-04-05
JPH04500754A (ja) 1992-02-13

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 19910315

AK Designated contracting states

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STAA Information on the status of an ep patent application or granted ep patent

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18W Application withdrawn

Withdrawal date: 19920627