EP0356367A2 - Milieu liquide pour l'infusion et pour la conservation d'organes - Google Patents
Milieu liquide pour l'infusion et pour la conservation d'organes Download PDFInfo
- Publication number
- EP0356367A2 EP0356367A2 EP89500080A EP89500080A EP0356367A2 EP 0356367 A2 EP0356367 A2 EP 0356367A2 EP 89500080 A EP89500080 A EP 89500080A EP 89500080 A EP89500080 A EP 89500080A EP 0356367 A2 EP0356367 A2 EP 0356367A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- infusion
- preservation
- liquid medium
- organs
- mannitol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Definitions
- the invention relates to a liquid medium for infusion in and preservation of solid organs, such as kidneys, pancreas, livers, hearts and others, being particularly appropriate for infusion in kidney blood vessels and subsequent preservation of kidneys.
- the organ Prior to performing a transplant, the organ is removed from the body of the donor. Where the organ is a kidney, it is preserved in an appropriate medium while the future recipient is being prepared. Finally, the organ is implanted in the recipient.
- infusion means the artificial circulation in the organ of a liquid medium having a composition appropriate for maintaining the functions of the organ.
- the liquid medium entrains the blood contained in the blood vessels, particularly the vessels for microcirculation, thereby avoiding the formation of microclots which would occlude the renal capillaries and would make the organ useless for transplant. This operation lasts for about 10 to 15 minutes.
- the infused organ is then stored in a sterile container at a temperature of about 4 o C such as to be submerged in a liquid having the same composition as the infusing liquid.
- the purpose of infusion and the subsequent cold storage is not only to make the organ viable for transplant, but furthermore to get the organ to work immediately it is transplanted.
- the aim, in the case of kidneys, is to avoid the post-transplant renal insufficiency which, regrettably, is still very frequent in human kidney transplants.
- Post-transplant oliguric renal insufficiency causes difficulties for the clinical management of the patient, obliges the use of dialysis while the oliguria lasts and makes kidney transplants more expensive, at the same time as there is a poorer prognosis for the functional survival of the graft.
- the liquid infusion medium regularly used in kidney transplant units is a solution proposed by Dr Collins, called “Eurocollins', the ionic composition of which imitates that of the intracellular medium and glucose is used to provide it with an osmolarity similar to that of the intra and extracellular medium.
- the "Eurocollins" liquid medium has the drawback that the glucose diffuses through the cellular membranes, causing intracellular oedema, which is one of the most important factors in the onset of the post-transplant renal insufficiency.
- the present invention proposes a liquid medium characterised in that it includes an amount of mannitol lying between 3.4 and 4.6 wt%, preferably between 4.0 and 4.4 wt%.
- the liquid medium comprises also an amount of sodium allopurinol lying between 0.025 and 0.1 wt%.
- mannitol is a hexatomic alcohol having the formula CH2OH-(CHOH)4-CH2OH.
- mannitol over glucose is that it does not diffuse through the cellular membrane, i.e., it is a good impermeabilising agent, whereby it maintains the tonicity of the extracellular medium and prevents the intracellular oedema mentioned above.
- mannitol is a potent scavenger of hydroxyl type free oxygen radicals.
- allopurinol is a potent inhibitor of the enzyme xanthinooxidase, whereby the degradation of the renal uric acid and, therefore, the production of the free oxygen radicals responsible for renal damage during the reinfusion of the organ with blood, particularly if the cold ischaemia exceeds 16 hours duration, is avoided.
- liquid medium containing mannitol but not including sodium allopurinol is useful in common clinical practice with regular cold ischaemia times, while the medium containing sodium allopurinol is used preferably with extended cold ischaemia periods.
- composition according to a centesimal formula of the medium of the invention is as follows: COMPOSITION 1 Mannitol.... 4.19 g Monopotassium phosphate.... 0.205 g Dipotassium phosphate... 0.907 g Potassium chloride.... 0.112 g Sodium bicarbonate.... 0.084 g Water, q.s. ad.... 100 ml
- composition, according to a centesimal formula, of a medium according to the invention containing sodium allopurinol is the following: COMPOSITION 2 Mannitol.... 4.19 g Monopotassium phosphate.... 0.205 g Dipotassium phosphate... 0.907 g Potassium chloride.... 0.112 g Sodium bicarbonate.... 0.084 g Sodium allopurinol.... 0.05 g Water, q.s. ad.... 100 ml
- the invention does not consist of a method of surgical or therapeutical treatment of the human or animal body, since the medium according to the invention has no properties to cure patients.
- the medium according to the invention is not a chemical product, since it is obtained physically, by way of mixtures and solutions.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES8802547 | 1988-08-16 | ||
ES8802547A ES2007994A6 (es) | 1988-08-16 | 1988-08-16 | Medio liquido para perfusion y preservacion de organos. |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0356367A2 true EP0356367A2 (fr) | 1990-02-28 |
EP0356367A3 EP0356367A3 (fr) | 1991-04-17 |
Family
ID=8257764
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19890500080 Withdrawn EP0356367A3 (fr) | 1988-08-16 | 1989-07-12 | Milieu liquide pour l'infusion et pour la conservation d'organes |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP0356367A3 (fr) |
ES (1) | ES2007994A6 (fr) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0664080A1 (fr) * | 1994-01-25 | 1995-07-26 | Kabushiki Kaisha Seitai Kagaku Kenkyusho | Méthode pour la préservation de tissus vivants et perfusat |
WO1996005727A1 (fr) * | 1994-08-18 | 1996-02-29 | The American National Red Cross | Procede de preparation d'organes en vue de leur cryopreservation et de leur transplantation subsequente |
WO1999002034A1 (fr) * | 1997-07-09 | 1999-01-21 | Wayne State University | Solution de lavage et de conservation pour organes de donneurs |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH683485A5 (fr) * | 1990-11-20 | 1994-03-31 | Pasteur Merieux Serums Vacc | Solutions de perfusion, de conservation et de reperfusion d'organes. |
BE1007500A3 (fr) * | 1992-09-18 | 1995-07-18 | Pasteur Merieux Serums Vacc | Solution de perfusion, de conservation et de reperfusion d'organes. |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD128072A1 (de) * | 1976-11-04 | 1977-10-26 | Werner Fuertig | Initialperfusionsloesung |
WO1986000812A1 (fr) * | 1984-07-30 | 1986-02-13 | Pharmacia Ab | Composition medicamenteuse ou medicament sous forme de kit utilises pour la prevention et le traitement de degats causes a des cellules ischemiques, et sa preparation |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE906129A (fr) * | 1986-12-30 | 1987-06-30 | Goffin Vincent | Solution de conservation d'organes. |
-
1988
- 1988-08-16 ES ES8802547A patent/ES2007994A6/es not_active Expired
-
1989
- 1989-07-12 EP EP19890500080 patent/EP0356367A3/fr not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD128072A1 (de) * | 1976-11-04 | 1977-10-26 | Werner Fuertig | Initialperfusionsloesung |
WO1986000812A1 (fr) * | 1984-07-30 | 1986-02-13 | Pharmacia Ab | Composition medicamenteuse ou medicament sous forme de kit utilises pour la prevention et le traitement de degats causes a des cellules ischemiques, et sa preparation |
Non-Patent Citations (2)
Title |
---|
THE LANCET, vol. 1, 12th May 1973, pages 1024-1028; S.A. SACKS et al.: "Canine kidney preservation using a new perfusate" * |
TRANSPLANTATION PROCEEDINGS, SECOND INTERNATIONAL CONGRESS ON CYCLOSPORINE, Washington, DC, 4th - 7th November 1987, vol. XX, no. 3, suppl. 3, pages 239-243, Grune & Stratton, Inc.; M.S. KARIM et al.: "Ameliorating cyclosporine nephrotoxicity with intravenous protective agents" * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5723282A (en) * | 1991-07-08 | 1998-03-03 | The American National Red Cross | Method of preparing organs for vitrification |
US5821045A (en) * | 1991-07-08 | 1998-10-13 | The American National Red Cross | Methods for removal of cryoprotectant from organs prior to transplantation |
US5962214A (en) * | 1991-07-08 | 1999-10-05 | The United States Of America As Represented By The American National Red Cross | Method of preparing tissues and cells for vitrification |
US6187529B1 (en) | 1991-07-08 | 2001-02-13 | The American National Red Cross | Method for preparing organs for transplantation after cryopreservation |
EP0664080A1 (fr) * | 1994-01-25 | 1995-07-26 | Kabushiki Kaisha Seitai Kagaku Kenkyusho | Méthode pour la préservation de tissus vivants et perfusat |
WO1996005727A1 (fr) * | 1994-08-18 | 1996-02-29 | The American National Red Cross | Procede de preparation d'organes en vue de leur cryopreservation et de leur transplantation subsequente |
WO1999002034A1 (fr) * | 1997-07-09 | 1999-01-21 | Wayne State University | Solution de lavage et de conservation pour organes de donneurs |
Also Published As
Publication number | Publication date |
---|---|
ES2007994A6 (es) | 1989-07-01 |
EP0356367A3 (fr) | 1991-04-17 |
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18D | Application deemed to be withdrawn |
Effective date: 19911018 |